CN103750847B - The collection container of mononuclearcell and Platelet-rich plasm - Google Patents
The collection container of mononuclearcell and Platelet-rich plasm Download PDFInfo
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- CN103750847B CN103750847B CN201410015216.5A CN201410015216A CN103750847B CN 103750847 B CN103750847 B CN 103750847B CN 201410015216 A CN201410015216 A CN 201410015216A CN 103750847 B CN103750847 B CN 103750847B
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- platelet
- outer tube
- interior pipe
- collection container
- mononuclearcell
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Abstract
The present invention is a kind of blood collection container, the particularly collection container of a kind of mononuclearcell and Platelet-rich plasm, comprise outer tube and interior pipe, support member is provided with in outer tube, interior pipe is located in outer tube by support member, outer tube wall and outer wall of inner tube close contact, but not locked, should keep easily interior pipe being extracted state; From bottom to top preset cell separation liquid, separation gel and anticoagulant successively in interior pipe, the tube wall above anticoagulant inlays hole plastics paste layer; Cover outer tube and interior pipe with plug after being evacuated in pipe simultaneously; Label on outer tube.Good effect of the present invention is as follows: 1, blood collector is divided into inner and outer pipes structure, has both been conducive to vacuum and has kept, be conducive to again interior pipe and operate separately.2, inner pipe wall inlays hole plastics paste layer, inner and outer pipes both can have been made to be communicated with and be all in vacuum state, be conducive to again the closed collection of centrifugal rear mononuclearcell and Platelet-rich plasm, reduce and infect probability.
Description
Technical field
The present invention is a kind of blood collection container, particularly the collection container of a kind of mononuclearcell and Platelet-rich plasm.
Background technology
Along with the development of laboratory medicine and Biotherapeutics technology, the various factors in the lymphocyte in mononuclear cell (CPT) and Platelet-rich plasm (PRP), except for except scientific research and Clinical Laboratory, also for various Biotherapeutics.
Mononuclearcell can be used for biological immune treatment by steps such as induction, activation and quantity amplifications under ultra clean environment.General method is extracted lymphocyte and antigen presenting cell, through In vitro culture, copy, activate, the process such as differentiation, its quantity and activity are largely increased, and then feed back in patient body, play antiviral and antitumor action.
Fester recovery and skin transplantation etc. that Platelet-rich plasm causes for osseous tissue recovery, burn treating, chronic ulcer and diabetes, play very effectively facilitation; In beauty treatment, PRP carries color for wrinkle reduction, Firm, remove eye pouch, alleviates cicatrix, changes pigmentation etc., also plays an important role.
So the extraction quality of mononuclearcell and Platelet-rich plasm, it is the key factor affecting its function and effect.
At present, although employ Special vacuum blood taking tube to gather mononuclearcell and Platelet-rich plasm, but no matter be the extraction of mononuclearcell or the extraction of Platelet-rich plasm, all present open-sky technique, the sample of collection is easy to contaminated, easily activate platelet simultaneously, finally cause unsatisfactory curative effect and cause untoward reaction because polluting, affect Biotherapeutics effect.
Summary of the invention
The object of the invention is collection container in order to overcome current CPT and PRP when extracting sample easily contaminated, platelet be easily activated and affect the defect of Biotherapeutics effect, invent a kind of open-sky technique that reduces and avoid the mononuclearcell of sample contaminated raising Biotherapeutics effect and the collection container of Platelet-rich plasm, this container is mainly used in the extraction of mononuclearcell and Platelet-rich plasm.
The object of the invention is to realize as follows: the collection container of described mononuclearcell and Platelet-rich plasm, comprise outer tube and interior pipe, support member is provided with in outer tube, interior pipe is located in outer tube by support member, outer tube wall and outer wall of inner tube close contact, but not locked, should keep easily interior pipe being extracted state; From bottom to top preset cell separation liquid, separation gel and anticoagulant successively in interior pipe, the tube wall above anticoagulant inlays hole plastics paste layer; Cover outer tube and interior pipe with plug after being evacuated in pipe simultaneously; Label on outer tube.
Described anticoagulant can be sprayed on the inner tube wall of more than separation gel.
The bottom of described outer tube and interior pipe can be round bottom also can be conical bottom.
Described outer tube adopts glass or plastic production, and described plastics comprise polyethylene terephthalate (PET), PEN (PEN) or polybutylene terephthalate (PBT) (PBT).
Described interior pipe adopts glass or plastic production, and described plastics comprise PET (PET), polypropylene (PP), polystyrene (PS), polyvinylchloride, acetal resin (POM), polyamide (PA), Merlon (PC) or acrylonitrile-butadiene-styrene (ABS) (ABS)
Described hole plastics paste layer adopts polypropylene (PP), polyethylene (PE) or polyvinylidene fluoride to make.
Described cell separation liquid selects monocyte separation medium, lymphocyte separation medium, Po1ymorphPrep separating medium or leukocyte separating medium.
Described separation gel selects the blood of proportion between 1.077-1.091 (serum) separation gel or the separation gel of resistance to irradiation.
Described anticoagulant selects heparinate, edta salt, sodium citrate salt, ACD liquid, CPD Precerving liquid, sodium citrate buffer solution or anticoagulant for storage of whole blood.
Described plug 8 adopts halogenated butyl rubber to make; Described plug 8 can be replaced aluminum plastic film.
Good effect of the present invention is as follows: 1, blood collector is divided into inner and outer pipes structure, has both been conducive to vacuum and has kept, be conducive to again interior pipe and operate separately.2, inner pipe wall inlays hole plastics paste layer, inner and outer pipes both can have been made to be communicated with and be all in vacuum state, be conducive to again the closed collection of centrifugal rear mononuclearcell and Platelet-rich plasm, reduce and infect probability.
Accompanying drawing explanation
Fig. 1 is structure chart of the present invention
Fig. 2 is state diagram in pipe after blood drawing
Fig. 3 is state diagram in centrifugal rear pipe
In figure: pipe 3 support member in 1 outer tube 2
4 cell separation liquid 5 separation gel 6 anticoagulant
7 hole plastics paste layer 8 plug 9 labels
10 blood 11 erythrocyte 12 mononuclearcells
13 Platelet-rich plasm 14 platelet-poor plasmas
Detailed description of the invention
As shown in Figure 1, the collection container of described mononuclearcell and Platelet-rich plasm, the collection container of described mononuclearcell and Platelet-rich plasm, comprise outer tube 1 and interior pipe 2, be provided with support member 3 in outer tube 1, interior pipe 2 is located in outer tube 1 by support member 3, outer tube wall and outer wall of inner tube close contact, but not locked, should keep easily interior pipe being extracted state; From bottom to top preset cell separation liquid 4, separation gel 5 and anticoagulant 6 successively in interior pipe, the tube wall above anticoagulant 6 inlays hole plastics paste layer 7; Cover outer tube 1 and interior pipe 2 with plug 8 after being evacuated in pipe simultaneously; Label 9 on outer tube 1.
Described anticoagulant 6 can be sprayed on interior pipe 2 inwall of separation gel more than 5.
The bottom of described outer tube 1 and interior pipe 2 can be round bottom also can be conical bottom.
Described outer tube 1 adopts glass or plastic production, and described plastics comprise polyethylene terephthalate (PET), PEN (PEN) or polybutylene terephthalate (PBT) (PBT).
Described interior pipe 2 adopts glass or plastic production, and described plastics comprise PET (PET), polypropylene (PP), polystyrene (PS), polyvinylchloride, acetal resin (POM), polyamide (PA), Merlon (PC) or acrylonitrile-butadiene-styrene (ABS) (ABS)
Described hole plastics paste layer 7 adopts polypropylene (PP), polyethylene (PE) or polyvinylidene fluoride to make.
Described cell separation liquid 4 selects monocyte separation medium, lymphocyte separation medium, PolymorphPrep separating medium or leukocyte separating medium.
Described separation gel 5 selects the blood of proportion between 1.077-1.091 (serum) separation gel or the separation gel of resistance to irradiation.
Described anticoagulant 4 selects heparinate, edta salt, sodium citrate salt, ACD liquid, CPD Precerving liquid, sodium citrate buffer solution or anticoagulant for storage of whole blood.
Described plug 8 adopts halogenated butyl rubber to make; Described plug 8 can be replaced aluminum plastic film.
When using this collection container to take a blood sample, first by blood taking needle, blood 10 is communicated with pipe in collection container 2, owing to being prefixed vacuum, so blood 10 constantly enters in interior pipe 2, till reaching preset suction amount.At this moment anticoagulant 6 is dissolved in blood 10 and plays blood coagulation resisting function (see Fig. 2).Gathering can be centrifugal immediately after blood, can extract centrifugal by interior pipe, also can inner and outer pipes centrifugal together.After centrifugal, because erythrocyte 11 proportion is larger, sink at the bottom of interior pipe pipe by centrifugal action, originally be positioned at cell separation liquid 4 bottom pipe due to rate of specific gravity be about 1.077 (being less than erythrocyte and separation gel proportion) moved to above separation gel 5, separation gel 5 proportion is greater than 1.077 and is less than 1.091 and between separating medium 4 and erythrocyte 11, mononuclearcell 12 proportion is less than 1.077 and be positioned at above separating medium 4 and form cloud cellular layer, Platelet-rich plasm 13 is positioned at mononuclearcell layer 12 top and has part and mononuclearcell ply, platelet-poor plasma 14 is then positioned at the top.At this moment, the position of mononuclearcell layer 12 and Platelet-rich plasm 13 just and the hole plastics paste layer 7 (see Fig. 3) in the same plane inlayed of inner pipe wall, hole plastics paste layer has significant hydrophobicity, when drawing mononuclearcell 12 or Platelet-rich plasm 13, directly can draw with injection pinprick open-work gap plastics paste layer 7, avoid and open plug and draw mononuclearcell or Platelet-rich plasm and the pollution that brings.
Claims (9)
1. a collection container for mononuclearcell and Platelet-rich plasm, is characterized in that: comprise outer tube and interior pipe, be provided with support member in outer tube, interior pipe is located in outer tube by support member, outer tube wall and outer wall of inner tube close contact, but not locked, should keep easily interior pipe being extracted state; From bottom to top preset cell separation liquid, separation gel and anticoagulant successively in interior pipe, the tube wall above anticoagulant inlays hole plastics paste layer; Cover outer tube and interior pipe with plug after being evacuated in pipe simultaneously; Label on outer tube.
2. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: described anticoagulant is sprayed on the inner tube wall of more than separation gel.
3. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: described outer tube adopts glass or plastic production.
4. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: described interior pipe adopts glass or plastic production.
5. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: described hole plastics paste layer adopts polypropylene, polyethylene or polyvinylidene fluoride to make.
6. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: described cell separation liquid selects monocyte separation medium, lymphocyte separation medium, PolymorphPrep separating medium or leukocyte separating medium.
7. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: the blood separating colloid that described separation gel selects proportion between 1.077-1.091 or the separation gel of resistance to irradiation.
8. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: described anticoagulant selects heparinate, edta salt, sodium citrate salt, ACD liquid, CPD Precerving liquid, sodium citrate buffer solution or anticoagulant for storage of whole blood.
9. the collection container of mononuclearcell according to claim 1 and Platelet-rich plasm, is characterized in that: described plug adopts halogenated butyl rubber to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410015216.5A CN103750847B (en) | 2014-01-10 | 2014-01-10 | The collection container of mononuclearcell and Platelet-rich plasm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410015216.5A CN103750847B (en) | 2014-01-10 | 2014-01-10 | The collection container of mononuclearcell and Platelet-rich plasm |
Publications (2)
| Publication Number | Publication Date |
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| CN103750847A CN103750847A (en) | 2014-04-30 |
| CN103750847B true CN103750847B (en) | 2015-09-30 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112016023475B1 (en) * | 2014-04-09 | 2022-05-31 | Greiner Bio-One Gmbh | RECEPTION UNIT TO RECEIVE BLOOD |
| CN105708478A (en) * | 2015-04-20 | 2016-06-29 | 李彦清 | platelet-rich plasma fused hyaluronic acid (PRP & HA) blood collection and preparation tube |
| CN104799869A (en) * | 2015-04-27 | 2015-07-29 | 广东体必康生物科技有限公司 | Vacuum blood collection tube for separating peripheral blood mononuclear cells |
| CN106139290B (en) * | 2016-08-01 | 2018-11-16 | 娄域峰 | Integrated autologous platelet rich plasma preparation suit |
| CN107760593A (en) * | 2016-08-19 | 2018-03-06 | 付士明 | The evacuated blood collection tube of storage and transport circle nucleic acid sample |
| CN107773251A (en) * | 2016-08-25 | 2018-03-09 | 付士明 | Closed extraction PRP evacuated blood collection tubes and its supporting apparatus |
| CN114098726A (en) * | 2021-10-25 | 2022-03-01 | 四川绵阳四0四医院 | Infant sample blood collection tube suitable for automatic instrument detection and manufacturing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH01202417A (en) * | 1988-02-08 | 1989-08-15 | Sato Kasei Kogyosho:Kk | Manufacture of blood-collecting pipe |
| US6911315B2 (en) * | 1997-11-24 | 2005-06-28 | David L. Rimm | Method for the detection, identification, enumeration and confirmation of virally infected cells and other epitopically defined cells in whole blood |
| CN202430219U (en) * | 2011-11-23 | 2012-09-12 | 付士明 | Vacuum blood collection tube for mononuclear cell |
| CN202430220U (en) * | 2011-12-05 | 2012-09-12 | 付士明 | Vacuum blood collecting tube for preplaced Percoll separating solution |
| CN202891950U (en) * | 2012-07-16 | 2013-04-24 | 付士明 | Vacuum blood collection tube for automatically extracting blood serum or blood plasma |
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