CN211999764U

CN211999764U - Cell culture bottle

Info

Publication number: CN211999764U
Application number: CN202020351730.7U
Authority: CN
Inventors: 束健
Original assignee: Nanjing Youyiyou Biotechnology Co ltd
Current assignee: Nanjing Youyiyou Biotechnology Co ltd
Priority date: 2020-03-19
Filing date: 2020-03-19
Publication date: 2020-11-24
Anticipated expiration: 2030-03-19

Abstract

The utility model provides a cell culture bottle relates to cell culture consumptive material field, the bottle comprises a bottle body, bottleneck and bottle lid, the inside of first passageway and second passageway is inlayed respectively and is had first sealing strip and second sealing strip, the below of first sealing strip and second sealing strip is equipped with first guide rail frame and second guide rail frame respectively, the both ends inside wall of bottle is connected respectively to the one end of first guide rail frame and second guide rail frame, the other end of first guide rail frame and second guide rail frame is equipped with first draw-in groove and second draw-in groove respectively in the horizontal direction, the bottom of bottle is equipped with cell integration mechanism, the both ends inside wall of bottle is connected to cell integration mechanism's both ends lateral wall, the material of cell integration mechanism's bottom is medical silica gel, the mobilizable inside of installing at the bottle of cell integration mechanism, can scrape the bottom of bottle and the cell of lateral wall down. The utility model discloses do not influence cell activity, can reduce the experiment cost, easy operation.

Description

Cell culture bottle

Technical Field

The utility model belongs to cell culture consumptive material field, concretely relates to cell culture bottle.

Background

In vitro culture of cells has become an essential element in life research and practice, and the types of cells cultured are numerous, ranging from viruses to bacteria and fungi, from human cells to animal cells and plant cells. Some cells and tissues can grow in suspension, but a significant proportion of mammalian cells require surface adherence. Therefore, in addition to good transparency and non-toxicity and sterility, the culture flask used for providing the in vitro culture environment of the cells needs to be subjected to surface modification treatment so as to be capable of adhering to the wall, dividing and growing. The method is divided into three categories according to the requirement of the cells on the adherence of the product: the general type is suitable for suspension culture of cells and tissues; the standard type has good cell adherence performance and is suitable for adherence growth of cells; the special surface contains nitrogen-containing functional groups, and can promote the adherence, growth and differentiation of certain special cells (such as tumor cells). For example, chinese patent publication No. cn201410088501.x includes: a culture chamber within a bottle, the bottle comprising a flat bottom wall, a flat top wall spaced apart from the bottom wall, and a side wall bridging the distance between the edges of the bottom wall and the top wall, the bottom wall having a growth surface on an upper side; an opening in the region of the side wall adjoining the flat top wall at a distance from the bottom wall; and a hollow cylindrical neck connected to the edge of the opening and oriented at an acute angle with respect to the growth surface so that a serological pipette, a scraper, a catheter or a longitudinally elongated device for adding or extracting material can be placed from the outside through the neck to the rear corner of the growth surface, and a cell culture bottle of chinese patent publication No. CN201621460980.4 comprising a cell culture bottle body provided with a screw hole and a suction cup provided with a rod body provided with an external thread matching the screw hole, the rod body being screwed into the screw hole.

When the existing cell culture bottle takes out cells growing in the cell culture bottle, adherent cells are separated from the surface of the cell culture bottle by cell digestive juice to achieve the purpose of cell separation, and when the cell state is relatively poor or the cells are not easy to passage and grow, the cell digestive juice can damage the cells to reduce the activity, and the experiment cost is high.

Therefore, it is urgently needed to provide a cell culture bottle which does not affect the cell activity, can reduce the experiment cost and is simple to operate.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a cell culture bottle aiming at the defects of the prior cell culture bottle.

The utility model provides a following technical scheme:

the utility model provides a cell culture bottle, includes bottle, bottleneck and bottle lid, the both ends inside wall of bottle is equipped with first passageway and second passageway respectively at length direction, first passageway with the inside of second passageway is inlayed respectively and is had first sealing strip and second sealing strip, first sealing strip with the below of second sealing strip is equipped with first guide rail frame and second guide rail frame respectively, first guide rail frame with the one end of second guide rail frame is connected respectively the both ends inside wall of bottle, first guide rail frame with the other end of second guide rail frame is equipped with first draw-in groove and second draw-in groove respectively at the horizontal direction.

The bottom of the bottle body is provided with a cell integration mechanism which is movably arranged in the bottle body and can scrape cells at the bottom and the side wall of the bottle body, the upper surface of the top of the cell integration mechanism is connected with the lower surfaces of a first connecting rod and a second connecting rod, the tops of the first connecting rod and the second connecting rod are respectively provided with a first through hole and a second through hole in the horizontal direction, a first sterile inserted rod penetrates through the first channel and the first through hole and is connected with the first connecting rod, a second sterile inserted rod penetrates through the second channel and the second through hole and is connected with the second connecting rod, the outer side walls of the first connecting rod and the second connecting rod are respectively connected with one end of a third connecting rod and one end of a fourth connecting rod, and the other end of the third connecting rod and the fourth connecting rod are respectively connected with the rotating shaft of a first pulley and the rotating shaft of a second pulley through a first connecting frame and a second connecting frame, the first clamping groove and the second clamping groove can accommodate the first pulley and the second pulley to slide respectively.

Preferably, the outer side wall of the first sealing strip and the outer side wall of the second sealing strip are respectively connected with the inner side wall of the first channel and the inner side wall of the second channel, and one end of the first sealing strip and one end of the second sealing strip are respectively connected with a first pulling strip and a second pulling strip outside the bottle body. The outer side walls of the two ends of the cell integration mechanism are connected with the inner side walls of the two ends of the bottle body, and the bottom of the cell integration mechanism is made of medical silica gel.

Furthermore, the first clamping groove and the second clamping groove are symmetrically arranged by taking the central axis of the bottle body in the vertical direction as a symmetry axis, and the first connecting rod and the second connecting rod are symmetrically arranged by taking the central axis of the bottle body in the vertical direction as a symmetry axis.

The utility model has the advantages that:

(1) the cell integrating mechanism is used for scraping the adherent cells from the bottom and the side wall of the bottle body, so that the use of cell digestive juice is avoided, the activity of the cells is not influenced, and the experiment cost is reduced.

(2) The first brace and the second brace are pulled in sequence, the first sealing strip and the second sealing strip are taken out from the first channel and the second channel, the first sterile inserted link is inserted into the first through hole in a penetrating mode through the first channel, the second sterile inserted link is inserted into the second through hole in a penetrating mode through the second channel, the first sterile inserted link and the second sterile inserted link are pulled horizontally, the first pulley and the second pulley slide in the first clamping groove and the second clamping groove, the cell integration mechanism is pulled to the near end of the bottle cap from the far end of the bottle cap, and the operation is simple.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic view of the structure of the bottle body of the present invention;

fig. 2 is a front view of the present invention;

FIG. 3 is a right side view of the cell integration mechanism of the present invention.

Labeled as: 1. a bottle body; 2. a bottleneck; 3. a bottle cap; 4. a first channel; 5. a first seal strip; 6. a second seal strip; 7. a first brace; 8. a second brace; 9. a first guide rail frame; 10. a second guide rail frame; 11. a cell integration mechanism; 12. a first connecting rod; 13. a second connecting rod; 14. a first through hole; 15. a first sterile insertion rod; 16. a second sterile plunger; 17. a third connecting rod; 18. a fourth connecting rod; 19. a first connection frame; 20. a second connection frame; 21. a first pulley; 22. a second pulley; 23. a second card slot; 24. medical silica gel.

Detailed Description

As shown in the figure, a cell culture bottle comprises a bottle body 1, a bottle neck 2 and a bottle cap 3, wherein the inner side walls of the two ends of the bottle body 1 are respectively provided with a first channel 4 and a second channel in the length direction, a first sealing strip 5 and a second sealing strip 6 are respectively embedded in the first channel 4 and the second channel, the outer side walls of the first sealing strip 5 and the second sealing strip 6 are respectively connected with the inner side walls of the first channel 4 and the second channel, and one end of the first sealing strip 5 and one end of the second sealing strip 6 are respectively connected with a first brace 7 and a second brace 8 outside the bottle body 1.

The lower parts of the first sealing strip 5 and the second sealing strip 6 are respectively provided with a first guide rail frame 9 and a second guide rail frame 10, one ends of the first guide rail frame 9 and the second guide rail frame 10 are respectively connected with the inner side walls at two ends of the bottle body 1, the other ends of the first guide rail frame 9 and the second guide rail frame 10 are respectively provided with a first clamping groove and a second clamping groove 23 in the horizontal direction, the first clamping groove and the second clamping groove 23 are symmetrically arranged by taking the central axis of the bottle body 1 in the vertical direction as a symmetry axis, the bottom of the bottle body 1 is provided with a cell integration mechanism 11, the outer side walls at two ends of the cell integration mechanism 11 are connected with the inner side walls at two ends of the bottle body 1, the bottom of the cell integration mechanism 11 is made of medical silica gel 24, the cell integration mechanism 11 is movably arranged in the bottle body 1, and cells at the bottom and.

The upper surface at the top of the cell integration mechanism 11 is connected with the lower surfaces of a first connecting rod 12 and a second connecting rod 13, the top of the first connecting rod 12 and the top of the second connecting rod 13 are respectively provided with a first through hole 14 and a second through hole in the horizontal direction, a first sterile insertion rod 15 penetrates through a first channel 4 and the first through hole 14 to be connected with the first connecting rod 12, a second sterile insertion rod 16 penetrates through the second channel and the second through hole to be connected with the second connecting rod 13, and the first connecting rod 12 and the second connecting rod 13 are symmetrically arranged by taking the central axis of the bottle body 1 in the vertical direction as the symmetry axis. The outer side walls of the first connecting rod 12 and the second connecting rod 13 are respectively connected with one ends of the third connecting rod 17 and the fourth connecting rod 18, the other ends of the third connecting rod 17 and the fourth connecting rod 18 are respectively connected with the rotating shaft of the first pulley 21 and the rotating shaft of the second pulley 22 through the first connecting frame 19 and the second connecting frame 20, and the first clamping groove and the second clamping groove 23 can respectively accommodate the first pulley 21 and the second pulley 22 to slide.

The utility model discloses a use-way does: unscrewing the bottle cap 3, injecting cells and culture solution into the bottle body 1 through the bottle mouth and the bottle neck 2, closing the bottle cap 3, putting the bottle cap into an incubator for culture, taking the cells out after the cells grow adherently for a proper time, sequentially pulling the first pull strip 7 and the second pull strip 8, taking the first sealing strip 5 and the second sealing strip 6 out of the first channel 4 and the second channel, inserting the first sterile insertion rod 15 into the first through hole 14 through the first channel 4, inserting the second sterile insertion rod 16 into the second through hole through the second channel, horizontally pulling the first sterile insertion rod 15 and the second sterile insertion rod 16, sliding the first pulley 21 and the second pulley 22 in the first clamping groove and the second clamping groove 23, pulling the cell integration mechanism 11 from the far end of the bottle cap 3 to the near end of the bottle cap 3, scraping the cells at the position where the bottom and the side wall of the bottle body 1 contact the cell integration mechanism 11 into the culture solution, the bottle cap 3 is opened to suck the mixed liquid, and then the cells can be fully sucked out.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell culture bottle comprises a bottle body, a bottle neck and a bottle cap and is characterized in that inner side walls at two ends of the bottle body are respectively provided with a first channel and a second channel in the length direction, a first sealing strip and a second sealing strip are respectively embedded in the first channel and the second channel, a first guide rail frame and a second guide rail frame are respectively arranged below the first sealing strip and the second sealing strip, one end of the first guide rail frame and one end of the second guide rail frame are respectively connected with the inner side walls at two ends of the bottle body, the other end of the first guide rail frame and the other end of the second guide rail frame are respectively provided with a first clamping groove and a second clamping groove in the horizontal direction, a cell integration mechanism is arranged at the bottom of the bottle body and movably arranged in the bottle body and can scrape cells at the bottom and the side walls of the bottle body, the upper surface at the top of the cell integration mechanism is connected with the lower surfaces of a first connecting rod and a second connecting rod, the top of the first connecting rod and the top of the second connecting rod are respectively provided with a first through hole and a second through hole in the horizontal direction, a first sterile inserted rod penetrates through the first channel and the first through hole is connected with the first connecting rod, a second sterile inserted rod penetrates through the second channel and the second through hole is connected with the second connecting rod, the first connecting rod and the outer side wall of the second connecting rod are respectively connected with one end of a third connecting rod and one end of a fourth connecting rod, the third connecting rod and the other end of the fourth connecting rod are respectively connected with the rotating shaft of the first pulley and the rotating shaft of the second pulley through a first connecting frame and a second connecting frame, and the first clamping groove and the second clamping groove can respectively accommodate the first pulley and the second pulley to slide.

2. The cell culture flask according to claim 1, wherein the outer side wall of the first sealing strip and the outer side wall of the second sealing strip are connected to the inner side wall of the first channel and the inner side wall of the second channel, respectively, and the first sealing strip and the second sealing strip are connected at one end to the outside of the flask body by a first pulling strip and a second pulling strip, respectively.

3. The cell culture flask of claim 2, wherein the outer sidewalls of the two ends of the cell integration mechanism are connected to the inner sidewalls of the two ends of the flask body, and the bottom of the cell integration mechanism is made of medical silica gel.

4. The cell culture flask according to claim 1, wherein the first and second engaging grooves are symmetrically disposed about a vertical central axis of the flask, and the first and second connecting rods are symmetrically disposed about the vertical central axis of the flask.