CN210085472U - Culture dish - Google Patents
Culture dish Download PDFInfo
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- CN210085472U CN210085472U CN201920408460.6U CN201920408460U CN210085472U CN 210085472 U CN210085472 U CN 210085472U CN 201920408460 U CN201920408460 U CN 201920408460U CN 210085472 U CN210085472 U CN 210085472U
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Abstract
The utility model relates to a culture dish, including the culture dish body the inside culture plate that is used for cell or microorganism to cultivate that sets up of culture dish body, the clearance is left with the bottom plate of culture dish body to the culture plate, culture plate one end is connected with culture dish body lateral wall, and the other end leaves the clearance with culture dish body lateral wall, leaves gapped culture plate tip with culture dish body lateral wall and upwards extends and form overflow baffle, culture dish body upper end is provided with the opening, bonds the ventilated membrane that can open at the opening part, the ventilated membrane is with culture dish body upper end opening closing cap still be equipped with the filling opening that is used for the liquid feeding on the culture dish body and be used for moving the liquid mouth that moves the liquid, the filling opening is used for filling liquid to the culture dish top, move the liquid mouth and be used for moving out liquid from the culture dish below. Compared with the prior art, the utility model discloses the culture dish structure sets up simply, and is with low costs, but mass production facilitates the use, can avoid cross infection, practices thrift the cultivation cost.
Description
Technical Field
The utility model relates to a culture dish.
Background
The traditional disease treatment depends on three large pillars of chemical small molecule drugs, biological drugs/products and medical equipment, and the three treatment modes depend on different technologies, production and service modes, and provide corresponding products and services required by large pharmaceutical companies, biotechnology companies and medical equipment companies respectively.
Cell therapy, which is receiving increasing attention as a revolutionary treatment modality in the medical health field, is considered to be the fourth major support for disease treatment. Cell therapy is mainly used to treat various diseases by restoring or building the functions of cells or organs of a patient after living cells are injected into a human body as a carrier. Cell therapy is a novel therapeutic technique, encompassing: 1) permanent cell replacement therapy, 2) tissue engineering (structural and functional cell populations to treat human organ dysfunction), 3) transient cell therapy to disrupt or reduce the progression of natural disease, 4) immunoregulatory cell therapy, cell therapy to protect cells and tissues at risk, 5) gene therapy (via cell delivery vehicles) and 6) cell carcinoid vaccines.
Using new technology and different types of cells, such as T cells, hematopoietic stem cells, mesenchymal stem cells, skeletal muscle stem cells, lymphocytes, pancreatic islet cellsCell and dendritic cell, cell therapy can be used for treating various diseases such as cancer, autoimmune diseases and infectious diseases, repairing spinal cord injury, reconstructing damaged cartilage in joints, improving immune system and treating neurological disorders, etc. In view of the excellent effects of the prior studies, many pharmaceutical companies and investors invest large amounts of money for the development and commercialization of cell therapy-type products. About 20 cell therapy drugs have been approved on the market, including those approved in 2018(autologous adipose stem cell therapy for the treatment of complex perianal fistulas-one of the most disabling complications of Crohn's disease), and approved in 2017
And
(autologous CAR-T cells are used to treat diffuse B-cell lymphoma, primary mediastinal B-cell lymphoma, and B-cell acute lymphocytic leukemia currently over 500 cell therapy therapies are in the clinical development phase and over 1,000 cell therapy therapies are in the preclinical evaluation phase.
Unlike traditional drug production, both the raw materials and the end products of cell therapy are living human cells. Cells grow in an artificial environment outside a human body, the production process is complex, the cells are very sensitive to environmental changes, and a plurality of expensive biological reagents and consumables are required to be added. Meanwhile, strict GMP standards are required to be followed in the production process, so that the investment of early-stage plants and equipment is abnormally high. Cell growth is a slow process, and each batch of samples must independently go through the entire manufacturing process, so that the equipment use turnover rate is low, and the production efficiency is seriously affected. Meanwhile, most manufacturing processes of currently approved cell therapy products on the market are highly manual. The requirement of manual operation on operators is high, the problems of recruitment of personnel and reduction of operation stability in preparation meeting are solved, the product quality risk is improved due to the fact that manual operation inevitably causes operation errors, the possibility of cell product pollution (people are the largest pollution source) is greatly increased due to the manual operation, and the principle of consistency after preparation is improved due to the manual operation is difficult to meet.
The cell therapy industry needs to find an economical, efficient, large-scale, stable, high-quality cell production model to be able to successfully commercialize. In order to meet the requirement of automatic cell culture, a novel culture dish for cell culture is needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a culture dish for overcoming the defects of the prior art.
The purpose of the utility model can be realized through the following technical scheme:
the utility model provides a culture dish, includes the culture dish body the inside culture plate that is used for cell or microorganism to cultivate that sets up of culture dish body, the clearance is left with the bottom plate of culture dish body to the culture plate, culture plate one end is connected with culture dish body lateral wall, and the other end leaves the clearance with culture dish body lateral wall, leaves gapped culture plate tip with culture dish body lateral wall and upwards extends formation overflow baffle, culture dish body upper end is provided with the opening, bonds the ventilated membrane that can open at the opening part, the ventilated membrane is with culture dish body upper end opening closing cap still be equipped with the filling opening that is used for the liquid feeding on the culture dish body and move the liquid mouth with being used for moving the liquid, the filling opening is used for to the culture dish top filling liquid, move the liquid mouth and be used for shifting out liquid from the culture dish below.
The utility model discloses an among them preferred embodiment, the filling opening all sets up with move the liquid mouth the upper end of culture dish body, the filling opening is located the culture plate top, move the liquid mouth and be located the top in clearance between culture plate and the culture dish body lateral wall, adopt top application of sample mode setting promptly.
The utility model discloses an among them preferred embodiment, the filling opening is the rubber buffer mouth with moving the liquid mouth, and when the liquid feeding or move the liquid, inside liquid feeding syringe needle or liquid moving needle can pass the filling opening or move the liquid mouth and get into the culture dish body, when not inserting liquid feeding syringe needle or moving the liquid syringe needle, the filling opening with move the liquid mouth and guarantee not intercommunicate between culture dish body inside and the outside.
In one preferred embodiment of the present invention, the overflow barrier is located below the air permeable membrane. Namely, after the ventilated membrane is opened, the cell sheet cultured on the culture plate is conveniently taken down.
In one preferred embodiment of the present invention, the culture plate is parallel to the bottom plate of the culture dish body.
In one preferred embodiment of the present invention, the culture dish body is a cube or a rectangular structure, the culture plate is a square or a rectangular structure, three sides of the culture plate are connected to the side wall of the culture dish body, and the other side of the culture plate is spaced from the side wall of the culture dish body and extends upward to form the overflow baffle. The upper part of the culture plate is used as a separation layer for culturing cells or microorganisms.
In one preferred embodiment of the present invention, the overflow baffle is parallel to the side wall of the culture dish body.
The utility model discloses an in one of them preferred embodiment, the filling opening is seted up respectively with moving the liquid mouth the diagonal department of culture dish body upper end, the ventilated membrane is located the filling opening and moves between the liquid mouth.
In one of the preferred embodiments of the present invention, the upper end of the culture dish body is provided with an opening, a step-like structure is formed at the opening, and the breathable film is adhered to the step-like structure.
In one preferred embodiment of the present invention, the edge of the breathable film is provided with a guiding label for conveniently opening the breathable film. The guide label can also be a suspended breathable silicon membrane, namely the guide label is not bonded with the culture dish body, and the whole breathable membrane can be conveniently torn by hand after the culture is finished.
In one preferred embodiment of the present invention, the gas permeable membrane is a silica gel gas permeable membrane, and the gas permeable membrane allows gas including oxygen, nitrogen and carbon dioxide to pass through, and viruses, bacteria and water vapor cannot pass through, thereby preventing bacteria and virus aerosol from entering.
In one preferred embodiment of the present invention, the culture dish body is made of polystyrene.
The utility model provides a culture dish is particularly useful for the cell treatment with must take off the culture that is used for the cell piece of treatment in whole. For example, the skin epidermal cell sheet can be used for treating skin problems such as burn and vitiligo, and the myocardial cell sheet can be used for treating myocardial tissue necrosis.
The whole culture of the cell sheet is carried out by using the culture dish provided by the utility model as follows:
1. culturing: the cells are placed on a culture plate for culture, wherein gas including oxygen, nitrogen and carbon dioxide can penetrate through the breathable film and can not pass through viruses, bacteria and water vapor, and then bacteria and virus aerosol can be prevented from entering.
2. Adding a culture medium: the disposable needle is used and inserted into the liquid adding port to add liquid, and in the liquid adding process, the waste culture solution enters the bottom plate of the culture dish through the overflow baffle plate, and the waste culture medium of the culture dish is discharged through overflow.
3. Removing the waste culture medium: the disposable needle is inserted into the liquid transfer port, and the waste liquid below the culture plate is removed.
4. Uncovering the ventilated membrane, and harvesting cell sheets: the gas permeable membrane is uncovered, and then the cell sheet on the culture plate is removed in a whole piece.
The utility model provides a culture dish generally uses as disposable article when using.
The utility model provides a culture dish still is fit for cooperation automatic culture and trades liquid equipment and uses. The automation of cell production has the following advantages: 1) the problems of uncertainty and preparation risks caused by human factors, such as different operation habits, operation errors and unstable operation, pollution risks, low efficiency and reduced product stability caused by the uncertainty and the preparation risks can be solved. 2) The method is beneficial to rapid replication and popularization, and the bottleneck of product consistency and stability of each preparation center after preparation is expanded is solved by standardized operation of an automatic system. 3) Effectively improves the yield, thereby reducing the preparation cost of the cell therapy product, leading the cell therapy to be used by more people and having great social benefit. 4) The teaching aid does not depend on a hand-held teaching mode of a person, but depends on the technical propagation of an automatic integrated system, and is favorable for protecting intellectual property rights. Specifically, the automatic culture system solves the problems of randomness of operation time of a manual process, strict requirements on a culture schedule, artificial misjudgment or misunderstanding, personnel difference, increase of pollution risks caused by personnel flow and the like, and becomes a new direction for development of cell therapy products.
Compared with the prior art, the utility model discloses the main advantage of culture dish embodies in following aspect:
1. the design of the openable breathable film is as follows: the culture space is totally closed during culture, only gas including oxygen and carbon dioxide can penetrate through the culture space, and viruses, bacteria and water vapor cannot penetrate through the culture space. The design enables cells of different patients to be cultured in the same incubator, avoids cross infection and saves culture cost.
2. The utility model discloses the design of culture dish overflow makes the liquid feeding separately with moving the liquid, does not have cross infection. The overflow ensures the liquid level height of the added culture medium, and meanwhile, the overflowed liquid plays a role in adjusting the humidity of the closed culture space.
3. The utility model discloses the culture dish structure sets up simply, and is with low costs, but mass production facilitates the use.
Drawings
FIG. 1 is a schematic front view, half-sectional view, of a culture dish in example 1;
FIG. 2 is a schematic top view of the culture dish according to example 1;
FIG. 3 is a schematic view showing the structure of a culture dish in use according to example 1;
FIG. 4 is a flowchart showing the overall culture process of a cell sheet in the culture dish in example 1.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
Examples
A culture dish, referring to FIGS. 1-3, comprises a culture dish body 1, a culture plate 2 for culturing cells or microorganisms is arranged inside the culture dish body 1, a gap is reserved between the culture plate 2 and the bottom plate of the culture dish body 1, one end of the culture plate 2 is connected with the side wall of the culture dish body 1, a gap is reserved between the other end of the culture plate 2 and the side wall of the culture dish body 1, the end part of the culture plate 2 which has a gap with the side wall of the culture dish body 1 extends upwards to form an overflow baffle plate 21, the upper end of the culture dish body 1 is provided with an opening, a liftable air-permeable membrane 3 is bonded at the opening, the air-permeable membrane 3 covers the opening at the upper end of the culture dish body 1, the culture dish body 1 is also provided with a liquid adding port 11 for adding liquid and a liquid transferring port 12 for transferring liquid, the filling port 11 is used for filling liquid above the culture plate 2, and the liquid transferring port 12 is used for transferring liquid from below the culture plate 2.
In this embodiment, filling opening 11 and transfer port 12 are all seted up the upper end of culture dish body 1, filling opening 11 is located 2 tops of culture plate, transfer port 12 is located the top in clearance between culture plate 2 and the culture dish body 1 lateral wall, adopts top application of sample mode setting promptly.
In this embodiment, filling opening 11 is the rubber buffer mouth with move liquid mouth 12, and when the liquid feeding or move the liquid, filling opening 11 or move liquid syringe needle 5 can pass through filling opening 11 or move liquid mouth 12 and get into culture dish body 1 inside, when not inserting the filling opening syringe needle or moving the liquid syringe needle, filling opening 11 and move liquid mouth 12 guarantee not intercommunicate between culture dish body 1 inside and the outside.
In this embodiment, the overflow baffle 21 is located below the gas permeable membrane 3. Namely, after the ventilated membrane is opened, the cell sheet 4 cultured on the culture plate is conveniently taken down.
In this embodiment, the culture plate 2 is parallel to the bottom plate of the culture dish body 1.
In this embodiment, the culture dish body 1 is square or cuboid structure, the culture plate 2 is square or rectangle structure, trilateral all being connected with culture dish body 1 lateral wall of culture plate 2, the another side leaves the clearance with culture dish body 1 lateral wall and upwards extends and form overflow baffle 21. The upper part of the culture plate 2 is used as a separation layer for culturing cells or microorganisms.
In this embodiment, the overflow baffle 21 is parallel to the side wall of the culture dish body 1.
In this embodiment, the filling opening 11 and the liquid transfer opening 12 are respectively arranged at the diagonal line of the upper end of the culture dish body 1, and the air-permeable membrane 3 is positioned between the filling opening 11 and the liquid transfer opening 12.
In this embodiment, the upper end of the culture dish body 1 is provided with an opening, a step-shaped structure is formed at the opening, and the breathable film 3 is bonded at the step-shaped structure.
In this embodiment, the edge of the air permeable membrane 3 is provided with a guiding label 31 for conveniently opening the air permeable membrane 3. The guiding label 31 can also be a suspended breathable silicon membrane, namely the part is not bonded with the culture dish body, and the whole breathable membrane can be conveniently torn by hand after the culture is finished.
In this embodiment, the gas permeable membrane 3 is a silica gel gas permeable membrane, and the gas permeable membrane 3 allows gas including oxygen, nitrogen and carbon dioxide to pass through, so that viruses, bacteria and water vapor cannot pass through, thereby preventing bacteria and virus aerosol from entering.
In this embodiment, the culture dish body 1 is made of polystyrene.
The culture dish provided in this embodiment is particularly suitable for culturing a cell sheet for cell therapy which must be peeled off in its entirety. For example, the skin epidermal cell sheet can be used for treating skin problems such as burn and vitiligo, and the myocardial cell sheet can be used for treating myocardial tissue necrosis.
Referring to fig. 4, the procedure for performing bulk culture of cell sheets using the culture dish provided in the present example was as follows:
1. culturing: the cells are placed on a culture plate for culture, wherein gas including oxygen, nitrogen and carbon dioxide can penetrate through the breathable film and can not pass through viruses, bacteria and water vapor, and then bacteria and virus aerosol can be prevented from entering.
2. Adding a culture medium: the disposable needle is used and inserted into the liquid adding port to add liquid, and in the liquid adding process, the waste culture solution enters the bottom plate of the culture dish through the overflow baffle plate, and the waste culture medium of the culture dish is discharged through overflow.
3. Removing the waste culture medium: the disposable needle is inserted into the liquid transfer port, and the waste liquid below the culture plate is removed.
4. Uncovering the ventilated membrane, and harvesting cell sheets: the gas permeable membrane is uncovered, and then the cell sheet on the culture plate is removed in a whole piece.
The petri dish provided in the embodiment is generally used as a disposable product when being used.
The culture dish provided in this embodiment is also suitable for being used with automatic culture solution changing equipment.
The embodiments described above are intended to facilitate the understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention according to the disclosure of the present invention.
Claims (10)
1. A culture dish comprises a culture dish body (1) and is characterized in that a culture plate (2) used for culturing cells or microorganisms is arranged inside the culture dish body (1), a gap is reserved between the culture plate (2) and a bottom plate of the culture dish body (1), one end of the culture plate (2) is connected with the side wall of the culture dish body (1), the other end of the culture plate is reserved with the side wall of the culture dish body (1) and is upwards extended to form an overflow baffle (21) at the end part of the culture plate (2) which is reserved with the gap on the side wall of the culture dish body (1),
an opening is arranged at the upper end of the culture dish body (1), a liftable breathable film (3) is bonded at the opening, the breathable film (3) seals the opening at the upper end of the culture dish body (1),
the culture dish body (1) is also provided with a liquid adding port (11) for adding liquid and a liquid transferring port (12) for transferring liquid,
the liquid filling port (11) is used for filling liquid above the culture plate (2), and the liquid transferring port (12) is used for transferring the liquid from the lower part of the culture plate (2).
2. A culture dish according to claim 1, wherein the filling opening (11) and the pipetting opening (12) are both open at the upper end of the culture dish body (1),
the liquid adding port (11) is positioned above the culture plate (2), and the liquid transferring port (12) is positioned above a gap between the culture plate (2) and the side wall of the culture dish body (1).
3. The culture dish of claim 1, wherein the liquid feeding port (11) and the liquid transferring port (12) are rubber plug ports, and when liquid is fed or transferred, a liquid feeding needle or a liquid transferring needle can penetrate through the liquid feeding port (11) or the liquid transferring port (12) to enter the interior of the culture dish body (1), and when the liquid feeding needle or the liquid transferring needle is not inserted, the liquid feeding port (11) and the liquid transferring port (12) ensure that the interior and the exterior of the culture dish body (1) are not communicated with each other.
4. A culture dish according to claim 1, wherein the overflow baffle (21) is located below the gas permeable membrane (3).
5. Dish according to claim 1, characterized in that the plate (2) is parallel to the bottom plate of the dish body (1).
6. The culture dish according to claim 1, wherein the culture dish body (1) is a cube or a cuboid structure, the culture plate (2) is a square or a rectangle structure, three sides of the culture plate (2) are connected with the side wall of the culture dish body (1), and the other side of the culture plate is separated from the side wall of the culture dish body (1) by a gap and extends upwards to form an overflow baffle (21).
7. A culture dish according to claim 1 or 5, wherein the overflow baffle (21) is parallel to the side wall of the culture dish body (1).
8. A culture dish according to claim 6, wherein the filling opening (11) and the pipetting opening (12) are respectively arranged at the diagonal of the upper end of the culture dish body (1), and the air-permeable membrane (3) is positioned between the filling opening (11) and the pipetting opening (12).
9. A culture dish according to claim 1, wherein the culture dish body (1) is provided with an opening at the upper end, a step-like structure is formed at the opening, and the gas permeable membrane (3) is adhered at the step-like structure.
10. A culture dish according to claim 1, characterized in that the edge of the gas permeable membrane (3) is provided with a guide label (31) facilitating the lifting of the gas permeable membrane (3) in its entirety.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920408460.6U CN210085472U (en) | 2019-03-28 | 2019-03-28 | Culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920408460.6U CN210085472U (en) | 2019-03-28 | 2019-03-28 | Culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210085472U true CN210085472U (en) | 2020-02-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920408460.6U Active CN210085472U (en) | 2019-03-28 | 2019-03-28 | Culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210085472U (en) |
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2019
- 2019-03-28 CN CN201920408460.6U patent/CN210085472U/en active Active
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Legal Events
| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210723 Address after: Room 2031-2033, building 201, Chunfang Road, Pudong New Area, China Patentee after: SHANGHAI SHANGRUI BIOLOGICAL PHARMACEUTICAL Co.,Ltd. Patentee after: SHANGHAI REMED BIOTECHNOLOGY Co.,Ltd. Address before: Room 2031-2033, building 201, Chunfang Road, Pudong New Area, China Patentee before: SHANGHAI SHANGRUI BIOLOGICAL PHARMACEUTICAL Co.,Ltd. Patentee before: SHANGHAI REMED BIOTECHNOLOGY Co.,Ltd. Patentee before: HEFEI XINRUI BIOTECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right |