CN210765348U - Large-scale umbilical cord mesenchymal stem cell liquid conversion and liquid conversion system - Google Patents
Large-scale umbilical cord mesenchymal stem cell liquid conversion and liquid conversion system Download PDFInfo
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- CN210765348U CN210765348U CN201921672390.1U CN201921672390U CN210765348U CN 210765348 U CN210765348 U CN 210765348U CN 201921672390 U CN201921672390 U CN 201921672390U CN 210765348 U CN210765348 U CN 210765348U
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- 239000007788 liquid Substances 0.000 title claims abstract description 62
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 35
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 title description 7
- 238000004113 cell culture Methods 0.000 claims abstract description 47
- 238000012546 transfer Methods 0.000 claims abstract description 22
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 15
- 239000012530 fluid Substances 0.000 claims abstract description 11
- 238000007789 sealing Methods 0.000 claims description 28
- 210000004027 cell Anatomy 0.000 claims description 18
- 238000000034 method Methods 0.000 abstract description 12
- 230000008569 process Effects 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 7
- 238000001802 infusion Methods 0.000 abstract description 5
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 230000008859 change Effects 0.000 description 12
- 230000001502 supplementing effect Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 4
- 210000004504 adult stem cell Anatomy 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
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- 230000000249 desinfective effect Effects 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 210000003981 ectoderm Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
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Abstract
The utility model belongs to the technical field of cell culture technology and specifically relates to a large-scale liquid-changing system for converting umbilical cord mesenchymal stem cells into liquid, the whole set of device of the utility model can be completely placed in a biosafety cabinet for operation, and can be sterilized except a peristaltic pump and a cell culture box, thereby preserving the aseptic requirements of the cell culture process; the solution bottle and the cell culture box are both externally connected with an air filter, so that the pressure balance in the whole operation process can be maintained, and the cell culture box cannot be damaged; corresponding rotational speed can be controlled through the liquid pumped in by the peristaltic pump, can reach fine at the uniform velocity effect to the liquid transfer in-process, also improved the fluid infusion efficiency simultaneously, shorten the fluid infusion time, play the primary role in the enlarged scale preparation process of mesenchymal stem cells from T175 blake bottle to cell culture box, this system connection is convenient, easily clean disinfection, operation process also can guarantee the aseptic of cell culture, is the important auxiliary device of cell culture box scale preparation mesenchymal stem cells.
Description
Technical Field
The utility model discloses cell culture technical field specifically is a scale umbilical cord mesenchymal stem cell changes liquid and trades liquid system.
Background
Mesenchymal Stem Cells (MSCs) are important members of the stem cell family, originating from early-developing mesoderm and ectoderm, and were first found in bone marrow, and are a group of adult stem cells with high self-renewal capacity and multipotentiality. With the continuous and intensive research on mesenchymal stem cells, the cell line has the advantages of immunoregulation, cell factor secretion, convenient material taking and the like, shows wider and wider application prospects in the fields of autoimmune diseases, regenerative medicine and the like, and becomes an ideal seed cell for cell therapy. At present, the adult stem cell is the most clinically applied and researched after the hematopoietic stem cell, and is also the most mature adult stem cell. The abundance of MSCs is very low, and in-vitro amplification culture is required to achieve the magnitude order of clinical application. Although research on MSCs has been carried out for a long time, the culture process is based on experimental research, and the shift from experimental research to large-scale production of MSCs requires establishment of standards or specifications for clinical MSCs culture according to the requirements of clinical application. In the large-scale preparation of the mesenchymal stem cells, a cell factory and a microcarrier are mainly adopted for amplification culture at present, and the cell factory is the most main large-scale culture carrier at present in the aspects of cost factors, simplicity and convenience in operation and stability of cell culture quality. Because the opening of the cell factory is small, the convenience of liquid supplement and liquid replacement and the prevention and treatment of pollution are one of the most concerned technical indexes of many users.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a scale umbilical cord mesenchymal stem cell changes liquid system to solve the present fluid infusion that proposes in the above-mentioned background art and cause the problem of pollution when changing the liquid.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a scale umbilical cord mesenchymal stem cell changes liquid system that changes liquid, includes solution bottle, peristaltic pump and cell culture case, the bottleneck department of solution bottle is provided with the sealing plug, two internal thread holes have been seted up on the sealing plug, and one of them internal thread downthehole in-connection has the breather pipe, a tip connection that solution bottle was kept away from to the breather pipe has first air cleaner, another internal thread downthehole in-connection has a commentaries on classics liquid pipe, change liquid union coupling in the pump head of peristaltic pump, change the liquid pipe jack of having seted up on the pump head, change liquid pipe and change liquid pipe jack grafting cooperation, cell culture case are provided with input tube and output tube, change the liquid pipe and pass and change liquid pipe jack and be connected in cell culture case's input tube, be connected with second air cleaner on cell culture case's.
Preferably, the joint of the vent pipe, the liquid transfer pipe and the sealing plug is provided with an external thread pipe, and the external thread pipe is matched with the internal thread hole in size and is in threaded connection with the internal thread hole.
Preferably, the top of the external thread pipe is provided with a limiting disc.
Preferably, the sealing plug is matched with the bottle mouth of the solution bottle in size in an inserting mode, and the sealing plug and the bottle mouth are matched in an inserting mode.
Preferably, a limiting cap is arranged at the top of the sealing plug, and the sealing plug and the limiting cap are coaxially arranged.
Preferably, the cell culture chamber employs a multi-layered cell factory.
Preferably, the first air filter and the vent pipe are of an integrally formed structure, and the second air filter and the output pipe of the cell culture box are of an integrally formed structure.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the whole set of device of the large-scale umbilical cord mesenchymal stem cell liquid-transferring and liquid-changing system can be completely placed in a biological safety cabinet for operation, and sterilization treatment can be performed except for a peristaltic pump and a cell culture box, so that the aseptic requirement of the cell culture process is saved; the solution bottle and the cell culture box are both externally connected with an air filter, so that the pressure balance in the whole operation process can be maintained, and the cell culture box cannot be damaged; the peristaltic pump pumps liquid to control corresponding rotating speed, so that a good uniform speed effect can be achieved in the liquid transfer process, the liquid supplementing efficiency is improved, and the liquid supplementing time is shortened. This fluid infusion trades liquid system plays the primary role in the enlarged scale preparation process of cell culture case from T175 blake bottle to mesenchymal stem cell, and this system connection is convenient, and easily cleaning and disinfecting, operation process also can guarantee the aseptic of cell culture, is the important auxiliary device of cell culture case scale preparation mesenchymal stem cell. And is easy to popularize and apply.
2. The joint of breather pipe and commentaries on classics liquid pipe and sealing plug all is provided with the external screw thread pipe among this scale umbilical cord mesenchymal stem cells liquid conversion system, and external screw thread pipe and the size looks adaptation in internal thread hole and both threaded connection conveniently install and dismantle, dismantle after the use and practice thrift the space, and the top of external screw thread pipe is provided with spacing dish, carries on spacingly, improves sealed effect.
3. The cell culture box in the large-scale umbilical cord mesenchymal stem cell liquid conversion system adopts a multi-layer cell factory, so that liquid is convenient to fill and empty, and the cell culture box can be used after being detached without any other accessories. First air cleaner is the integrated into one piece structure with the breather pipe, and second air cleaner is the integrated into one piece structure with the output tube of cell culture case, improves the stability of structure.
4. The sealing plug in this scale umbilical cord mesenchymal stem cell changes liquid system and the cooperation of pegging graft of the size looks adaptation of the bottleneck of solution bottle and both conveniently installs and dismantles. The top of sealing plug is provided with spacing cap, and the sealing plug sets up with spacing cap is coaxial, and the spacing cap of setting can improve sealed effect, conveniently takes out the sealing plug simultaneously.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic view of the decomposition structure of the solution bottle of the present invention;
fig. 3 is a schematic structural view of the vent pipe of the present invention.
In the figure: 1. a solution bottle; 11. a breather pipe; 111. an externally threaded tube; 112. a limiting disc; 12. a first air filter; 13. a liquid transferring pipe; 14. a bottle mouth; 15. a sealing plug; 16. a limiting cap; 17. an internally threaded bore; 2. a peristaltic pump; 21. a pump head; 22. a liquid transfer pipe jack; 3. a cell incubator; 31. a second air filter; 32. an input tube; 33. and (5) outputting the product.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Referring to fig. 1-3, the present invention provides the following technical solutions:
a scale umbilical cord mesenchymal stem cell liquid transferring and changing system is shown in figures 1 and 2 and comprises a solution bottle 1, a peristaltic pump 2 and a cell culture box 3, wherein a sealing plug 15 is arranged at a bottle opening 14 of the solution bottle 1, two internal thread holes 17 are formed in the sealing plug 15, one of them internal thread hole 17 in-connection has breather pipe 11, a tip connection that solution bottle 1 was kept away from to breather pipe 11 has first air cleaner 12, another internal thread hole 17 in-connection has liquid pipe 13 of changeing, change liquid pipe 13 and connect in the pump head 21 of peristaltic pump 2, pump head 21 is last to have seted up and to change liquid pipe jack 22, change liquid pipe 13 and change the cooperation of pegging graft of liquid pipe jack 22, cell incubator 3 is provided with input tube 32 and output tube 33, change liquid pipe 13 and pass and change liquid pipe jack 22 and be connected in on cell incubator 3's input tube 32, be connected with second air cleaner 31 on cell incubator 3's the output tube 33.
Furthermore, as shown in fig. 3, the joints of the vent pipe 11, the liquid transfer pipe 13 and the sealing plug 15 are provided with external threaded pipes 111, the external threaded pipes 111 are matched with the internal threaded holes 17 in size and are in threaded connection with the internal threaded holes 17, so that the mounting and dismounting are convenient, the dismounting after use is performed, the space is saved, and the top of the external threaded pipes 111 is provided with a limiting disc 112 for limiting, so that the sealing effect is improved.
It is worth mentioning that the sealing plug 15 is matched with the bottle mouth 14 of the solution bottle 1 in size and the sealing plug and the bottle mouth are in plug-in fit, so that the installation and the disassembly are convenient. The top of sealing plug 15 is provided with spacing cap 16, and sealing plug 15 and the coaxial setting of spacing cap 16, the spacing cap 16 that sets up can improve sealed effect, conveniently takes out sealing plug 15 simultaneously.
In addition, the cell culture box 3 adopts a multi-layer cell factory, so that liquid is convenient to fill and drain, and the cell culture box can be used after being disassembled without any other accessories. The first air filter 12 and the air pipe 11 are integrally formed, and the second air filter 31 and the output pipe 33 of the cell incubator 3 are integrally formed, so that the structural stability is improved.
The capacity of solution bottle 1 among the scale umbilical cord mesenchymal stem cell conversion liquid system of this embodiment is 5L, once can the volume contain stem cell's complete culture solution 4L, satisfy 2 cell culture case 3's culture solution requirement, 3 culture solution use amount 2L of every cell culture case, the external first air cleaner 12 of breather pipe 11, its effect is 2 pump income culture solution in-process at peristaltic pump, the filtration that the outer air passes through air cleaner maintains solution bottle pressure balance, the pump head 21 of the middle access peristaltic pump 2 of commentaries on classics liquid pipe 13, control rotational speed can be at the uniform velocity with containing in the umbilical cord mesenchymal stem cell culture solution pump goes into cell culture case 3. The other interface of the cell culture box 3 is connected with a sterile second air filter 31 through a connector, and the sterile second air filter is used for maintaining the pressure balance in the cell factory in the processes of pumping culture solution, supplementing solution and replacing solution, and preventing the cell factory from being damaged due to overlarge pressure; or continuously producing CO during cell culture due to cell growth and metabolism2The pH concentration and pressure balance during the culture were maintained by the filter.
The whole set of device of the large-scale umbilical cord mesenchymal stem cell liquid-transferring system can be completely placed in a biosafety cabinet for operation, and can be sterilized except for a peristaltic pump and a cell culture box 3, so that the aseptic requirement of the cell culture process is saved; the solution bottle 1 and the cell culture box 3 are both externally connected with an air filter, so that the pressure balance in the whole operation process can be maintained, and the cell culture box 3 cannot be damaged; corresponding rotating speed can be controlled by pumping liquid into the peristaltic pump 2, a good uniform action can be achieved in the liquid transfer process, the liquid supplementing efficiency is improved, and the liquid supplementing time is shortened. This fluid infusion trades liquid system plays the primary role in the scale preparation process of enlargiing from T175 blake bottle to cell culture case 3 to mesenchymal stem cell, and this system connection is convenient, easily clean disinfection, and operation process also can guarantee the aseptic of cell culture, is the important auxiliary device of 3 scale preparation mesenchymal stem cells of cell culture case. And is easy to popularize and apply.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. A scale umbilical cord mesenchymal stem cell liquid transfer and liquid transfer system is characterized in that: the device comprises a solution bottle (1), a peristaltic pump (2) and a cell culture box (3), wherein a sealing plug (15) is arranged at a bottle opening (14) of the solution bottle (1), two internal threaded holes (17) are formed in the sealing plug (15), an air pipe (11) is connected in one internal threaded hole (17), one end, far away from the solution bottle (1), of the air pipe (11) is connected with a first air filter (12), a liquid transfer pipe (13) is connected in the other internal threaded hole (17), the liquid transfer pipe (13) is connected to a pump head (21) of the peristaltic pump (2), a liquid transfer pipe jack (22) is formed in the pump head (21), the liquid transfer pipe (13) is in inserting fit with the liquid transfer pipe jack (22), the cell culture box (3) is provided with an input pipe (32) and an output pipe (33), and the liquid transfer pipe (13) penetrates through the liquid transfer pipe jack (22) and is connected to the input pipe (32) of the cell culture box (3, and a second air filter (31) is connected to an output pipe (33) of the cell culture box (3).
2. The scaled umbilical cord mesenchymal stem cell fluid transfer system of claim 1, wherein: the joint of the vent pipe (11) and the liquid transfer pipe (13) and the sealing plug (15) is provided with an external threaded pipe (111), and the external threaded pipe (111) is matched with the size of the internal threaded hole (17) and is in threaded connection with the internal threaded hole.
3. The scaled umbilical cord mesenchymal stem cell fluid transfer system of claim 2, wherein: the top of the external thread pipe (111) is provided with a limiting disc (112).
4. The scaled umbilical cord mesenchymal stem cell fluid transfer system of claim 1, wherein: the sealing plug (15) is matched with the bottle mouth (14) of the solution bottle (1) in a size-adaptive manner in an inserting manner.
5. The scaled umbilical cord mesenchymal stem cell fluid transfer system of claim 1, wherein: the top of the sealing plug (15) is provided with a limiting cap (16), and the sealing plug (15) and the limiting cap (16) are coaxially arranged.
6. The scaled umbilical cord mesenchymal stem cell fluid transfer system of claim 1, wherein: the cell incubator (3) employs a multi-layered cell factory.
7. The scaled umbilical cord mesenchymal stem cell fluid transfer system of claim 1, wherein: the first air filter (12) and the air pipe (11) are of an integrated structure, and the second air filter (31) and the output pipe (33) of the cell culture box (3) are of an integrated structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921672390.1U CN210765348U (en) | 2019-10-08 | 2019-10-08 | Large-scale umbilical cord mesenchymal stem cell liquid conversion and liquid conversion system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921672390.1U CN210765348U (en) | 2019-10-08 | 2019-10-08 | Large-scale umbilical cord mesenchymal stem cell liquid conversion and liquid conversion system |
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| CN210765348U true CN210765348U (en) | 2020-06-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112574961A (en) * | 2020-12-28 | 2021-03-30 | 兆丰华生物科技(南京)有限公司 | Preparation method of cell vaccine antigen |
| CN114317395A (en) * | 2021-12-31 | 2022-04-12 | 中国肉类食品综合研究中心 | Adherent cell large-scale culture process |
-
2019
- 2019-10-08 CN CN201921672390.1U patent/CN210765348U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112574961A (en) * | 2020-12-28 | 2021-03-30 | 兆丰华生物科技(南京)有限公司 | Preparation method of cell vaccine antigen |
| CN114317395A (en) * | 2021-12-31 | 2022-04-12 | 中国肉类食品综合研究中心 | Adherent cell large-scale culture process |
| CN114317395B (en) * | 2021-12-31 | 2024-04-02 | 中国肉类食品综合研究中心 | Large-scale culture process for adherent cells |
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Effective date of registration: 20240705 Address after: No.245, Renmin East Road, Panlong District, Kunming, Yunnan 650031 Patentee after: Kunming Yan'an Hospital (Kunming Cadre Sanatorium) Country or region after: China Address before: No. 2404, building 2, Tanhua landscape garden, No. 1, Jingtai street, Panlong District, Kunming, Yunnan 650000 Patentee before: Meng Mingyao Country or region before: China |
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