CN213172391U

CN213172391U - A inhale waste liquid device for cell culture

Info

Publication number: CN213172391U
Application number: CN202021479036.XU
Authority: CN
Inventors: 董俊一
Original assignee: Taicang Zexin Biotechnology Co Ltd
Current assignee: Taicang Zexin Biotechnology Co Ltd
Priority date: 2020-07-24
Filing date: 2020-07-24
Publication date: 2021-05-11
Anticipated expiration: 2030-07-24

Abstract

The utility model provides a inhale waste liquid device for cell culture, includes waste liquid bottle, waste liquid buffer bottle, aseptic connecting pipe, elbow pipette, negative pressure suction pump, bears the seat, the waste liquid bottle passes through aseptic connecting pipe with the waste liquid buffer bottle and is connected, the elbow pipette passes through aseptic connecting pipe and connects on the waste liquid bottle, the negative pressure suction pump passes through aseptic connecting pipe and connects on the waste liquid buffer bottle, waste liquid buffer bottle and negative pressure suction pump establish and bear on the seat. Simple structure, reasonable, the flexible use is convenient, easily washs, buffering part and anti-backflow part in addition, both can the protection device, can reduce cell culture's pollution again, and the device removes in a flexible way, and it is more convenient to use.

Description

A inhale waste liquid device for cell culture

Technical Field

The utility model belongs to the technical field of cell culture, specifically, relate to a inhale waste liquid device for cell culture.

Background

Cell culture refers to all in vitro cultures, and means that tissues are taken out of a living organism and incubated under specific in vivo conditions such as a simulated physiological environment in vivo to survive and grow. The growth of cells requires a nutrient environment and the nutrient medium used to maintain cell growth is called the culture medium. The culture medium can be divided into a liquid culture medium and a solid culture medium according to its physical state. The liquid culture medium is used for large-scale industrial production and research work of basic theories such as physiological metabolism and the like.

At the cell culture liquid medium culture in-process, need collect the waste liquid that the cell culture in-process produced, cell culture waste liquid collection device among the prior art is taking place the phenomenon that the waste liquid blockked up easily with the leading-in waste liquid case in-process of waste liquid, simultaneously, it is inconvenient to wash the waste liquid case, leads to waste liquid incasement wall to pile up a large amount of dirt for a long time easily, influences the use of waste liquid case, consequently, it is very necessary to design a cell culture waste liquid collector who facilitates the use.

SUMMERY OF THE UTILITY MODEL

Utility model purpose: in order to overcome the defects, the utility model aims to provide a waste liquid suction device for cell culture.

The technical scheme is as follows: the utility model provides a inhale waste liquid device for cell culture, include waste liquid bottle, waste liquid buffer bottle, aseptic connecting pipe, elbow pipette, negative pressure suction pump, bear the seat, the waste liquid bottle passes through aseptic connecting pipe with the waste liquid buffer bottle and is connected, the elbow pipette passes through aseptic connecting pipe and connects on the waste liquid bottle, the negative pressure suction pump passes through aseptic connecting pipe and connects on the waste liquid buffer bottle, waste liquid buffer bottle and negative pressure suction pump are established on bearing the seat. A inhale waste liquid device for cell culture, through the negative pressure suction pump waste liquid bottle, form the negative pressure in the waste liquid buffer bottle, suction from the blake bottle to the waste liquid through aseptic connecting pipe and elbow pipette, the use of waste liquid buffer bottle can have certain buffering to the waste liquid of inspiration, avoid the waste liquid to enter into in the negative pressure suction pump, can prolong the life of negative pressure suction pump, the waste liquid bottle makes the waste liquid device of inhaling to wash easily with the design of waste liquid bottle and waste liquid buffer bottle, the use of elbow pipette is changed and is inhaled the waste liquid completely from the blake bottle, do not influence the growth of cell, the design bears the seat and for make full use of space, make the device compacter, can also make the device conveniently remove simultaneously, it is more convenient to use.

Furthermore, according to the waste liquid suction device for cell culture, the overflow valve is arranged in the waste liquid buffer bottle and connected to the sterile connecting pipe connected with the negative pressure suction pump. The design of the overflow valve prevents the waste liquid from entering the negative pressure suction pump and damaging the negative pressure suction pump.

Furthermore, in the above waste liquid suction device for cell culture, the sterile connecting pipe, which connects the waste liquid buffer bottle and the negative pressure suction pump, is provided with a gas filter head. The design of the gas filter head prevents the negative pressure suction pump from polluting bacteria, waste liquid bottles and waste liquid buffer bottles and further polluting cultured cells.

Further, in the above waste liquid suction device for cell culture, the waste liquid bottle is provided with a first bottle stopper, and the bottle stopper is provided with a first connection hole; and a second bottle plug is arranged on the waste liquid buffer bottle, and a second connecting hole is formed in the second bottle plug. The design of bottle plug makes things convenient for the washing of waste liquid bottle and waste liquid buffer bottle, makes things convenient for aseptic connecting pipe to couple together elbow pipette and negative pressure suction pump through the connecting hole on the bottle plug simultaneously, forms a confined negative pressure environment.

Furthermore, in the above waste liquid suction device for cell culture, the aseptic connecting tube connecting the waste liquid bottle and the elbow pipette is provided with a one-way valve. The design of check valve prevents that the waste liquid in the elbow pipette from flowing backwards to the blake bottle, reduces the pollution risk.

Further, foretell a inhale waste liquid device for cell culture, the elbow pipette includes pipette body, elbow, the elbow is connected to pipette body front end. The elbow design convenient to use of elbow pipette is nimble, can reach diversified operation, absorbs the waste liquid more thoroughly from the blake bottle, reduces the residue of waste liquid, and the remaining of waste liquid can influence the growth of cell.

Further, foretell a inhale waste liquid device for cell culture, the pipette body includes thick pipe portion and tubule portion, thick pipe portion connects the tubule portion, the elbow is connected to the tubule portion. The design of thick pipe portion and tubule portion, the vacuum suction pump suction pressure can be reduced in thick pipe portion, also be difficult for producing the jam, and the pressure of elbow can be reduced to the tubule portion, reduces the pollution risk.

Furthermore, above-mentioned be used for waste liquid device that inhales of cell culture, the contained angle between tubule portion and elbow is the ℃. The elbow can be easier to enter the culture bottle to absorb the waste liquid.

Further, foretell a inhale waste liquid device for cell culture, the waste liquid bottle is equipped with first liquid outlet, be equipped with the second liquid outlet on the waste liquid buffer bottle, be equipped with first valve on the first liquid outlet, be equipped with the second valve on the second liquid outlet. The design of the liquid outlet and the valve can facilitate the timely removal of the waste liquid from the waste liquid bottle, and prevent the waste liquid from overflowing in the waste liquid buffer bottle and the waste liquid bottle to cause machine pollution and damage.

Furthermore, according to the waste liquid suction device for cell culture, the lower end of the bearing seat is provided with the self-locking universal wheel. The design of the self-locking universal wheel can lead the waste liquid suction device to move freely, and the use is convenient.

Above-mentioned technical scheme can find out, the utility model discloses following beneficial effect has: a inhale waste liquid device for cell culture, simple structure, it is reasonable, high durability and convenient use, low application cost, good adaptability, the combined use of waste liquid buffer bottle and overflow valve has prevented in the waste liquid enters into the vacuum aspiration pump in this device, damage the vacuum aspiration pump, and simultaneously, the design of check valve prevents that the waste liquid from flowing backwards and entering into the blake bottle, cause the pollution to the cell of cultivateing, the design of waste liquid bottle and waste liquid buffer bottle also makes and inhales the waste liquid device and washs easily, the convenient to use of elbow pipette is nimble, waste liquid to in the blake bottle absorbs more thoroughly, do not influence the growth of cell, the design make full use of space of bearing seat, make the device compacter, can also make the device conveniently remove simultaneously, it is more convenient to use.

Drawings

FIG. 1 is a schematic structural diagram of a waste liquid suction device for cell culture according to the present invention.

In the figure: the device comprises a waste liquid bottle 1, a waste liquid buffer bottle 2, an aseptic connecting pipe 3, a pipette with 4 elbows, a negative pressure suction pump 5, a bearing seat 6, a first bottle plug 11, a first connecting hole 12, a one-way valve 13, a first liquid outlet 14, a first valve 15, a second bottle plug 21, a second connecting hole 22, an overflow valve 23, a gas filter head 24, a second liquid outlet 25, a second valve 26, a pipette body 41, an elbow 42, a thick pipe part 43, a thin pipe part 44 and a self-locking universal wheel 61.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, unless otherwise specified, "a plurality" means two or more unless explicitly defined otherwise.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Examples

The waste liquid sucking device for cell culture shown in fig. 1 comprises a waste liquid bottle 1, a waste liquid buffer bottle 2, an aseptic connecting pipe 3, an elbow pipette 4, a negative pressure suction pump 5 and a bearing seat 6, wherein the waste liquid bottle 1 is connected with the waste liquid buffer bottle 2 through the aseptic connecting pipe 3, the elbow pipette 4 is connected to the waste liquid bottle 1 through the aseptic connecting pipe 3, the negative pressure suction pump 5 is connected to the waste liquid buffer bottle 2 through the aseptic connecting pipe 3, the waste liquid bottle 1, the waste liquid buffer bottle 2 and the negative pressure suction pump 5 are arranged on the bearing seat 6, and the lower end of the bearing seat 6 is provided with a self-locking universal wheel 61.

In addition, an overflow valve 23 is arranged in the waste liquid buffer bottle 2, and the overflow valve 23 is connected to the sterile connecting pipe 3 connected with the negative pressure suction pump 5.

And a gas filter head 24 is arranged on the sterile connecting pipe 3 which connects the waste liquid buffer bottle 2 and the negative pressure suction pump 5.

In addition, a first bottle stopper is arranged on the waste liquid bottle 1, and a first connecting hole is formed in the first bottle stopper; and a second bottle plug is arranged on the waste liquid buffer bottle 2, and a second connecting hole is formed in the second bottle plug.

And a one-way valve 13 is arranged on the sterile connecting pipe 3 which connects the waste liquid bottle 1 and the elbow pipette 4.

Wherein, elbow pipette 4 includes pipette body, elbow 42 is connected to the pipette body front end. The pipette body includes a thick tube part 43 and a thin tube part 44, the thick tube part 43 being connected to the thin tube part 44, and the thin tube part 44 being connected to the elbow 42. The angle between the narrow tube portion 44 and the elbow 42 is 45 ℃.

Meanwhile, the waste liquid bottle 1 is provided with a first liquid outlet 14, the waste liquid buffer bottle 2 is provided with a second liquid outlet, the first liquid outlet 14 is provided with a first valve 15, and the second liquid outlet is provided with a second valve.

The working principle is as follows: when absorbing the waste liquid to the blake bottle, waste liquid bottle 1 and waste liquid buffer bottle 2 are at first plugged with first bottle plug and second bottle plug respectively tightly, each waste liquid bottle 1, waste liquid buffer bottle 2, aseptic connecting pipe 3, elbow pipette 4 and negative pressure suction pump 5 each part are connected well to reuse aseptic connecting pipe 3, and inspect the gas tightness of inhaling the waste liquid device, start negative pressure suction pump 5, put elbow pipette 4 into blake bottle 7 again, absorb the waste liquid in the blake bottle, when finishing absorbing the waste liquid, withdraw elbow pipette 4 from blake bottle 7, close negative pressure suction pump 5 again, later flow the waste liquid in waste liquid bottle 1, waste liquid buffer bottle 2 through the liquid outlet, inhale the waste liquid device again and wash, the sterilization.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications can be made without departing from the principles of the present invention, and these modifications should also be regarded as the protection scope of the present invention.

Claims

1. A inhale waste liquid device for cell culture which characterized in that: including waste liquid bottle (1), waste liquid buffer bottle (2), aseptic connecting pipe (3), elbow pipette (4), vacuum aspiration pump (5), bear seat (6), waste liquid bottle (1) is connected through aseptic connecting pipe (3) with waste liquid buffer bottle (2), elbow pipette (4) are connected on waste liquid bottle (1) through aseptic connecting pipe (3), vacuum aspiration pump (5) are connected on waste liquid buffer bottle (2) through aseptic connecting pipe (3), waste liquid bottle (1), waste liquid buffer bottle (2) and vacuum aspiration pump (5) are established on bearing seat (6).

2. The waste liquid suction apparatus for cell culture according to claim 1, wherein: an overflow valve (23) is arranged in the waste liquid buffer bottle (2), and the overflow valve (23) is connected to a sterile connecting pipe (3) connected with the negative pressure suction pump (5).

3. The waste liquid suction apparatus for cell culture according to claim 2, wherein: and a gas filter head (24) is arranged on the sterile connecting pipe (3) which connects the waste liquid buffer bottle (2) and the negative pressure suction pump (5).

4. The waste liquid suction apparatus for cell culture according to claim 1, wherein: the waste liquid bottle (1) is provided with a first bottle stopper, and the first bottle stopper is provided with a first connecting hole; and a second bottle plug is arranged on the waste liquid buffer bottle (2), and a second connecting hole is formed in the second bottle plug.

5. The waste liquid suction apparatus for cell culture according to claim 1, wherein: the aseptic connecting pipe (3) connected with the waste liquid bottle (1) and the elbow pipette (4) is provided with a one-way valve (13).

6. The waste liquid suction apparatus for cell culture according to claim 1, wherein: the elbow pipette (4) comprises a pipette body and an elbow (42), and the front end of the pipette body is connected with the elbow (42).

7. The waste liquid suction device for cell culture according to claim 6, wherein: the pipette body (41) comprises a thick pipe part (43) and a thin pipe part (44), wherein the thick pipe part (43) is connected with the thin pipe part (44), and the thin pipe part (44) is connected with an elbow (42).

8. The waste liquid suction apparatus for cell culture according to claim 7, wherein: the included angle between the thin pipe part (44) and the elbow (42) is 45 ℃.

9. The waste liquid suction apparatus for cell culture according to claim 3, wherein: waste liquid bottle (1) is equipped with first liquid outlet (14), be equipped with the second liquid outlet on waste liquid buffer bottle (2), be equipped with first valve (15) on first liquid outlet (14), be equipped with the second valve on the second liquid outlet.

10. The waste liquid suction apparatus for cell culture according to claim 3, wherein: the lower end of the bearing seat (6) is provided with a self-locking universal wheel (61).