CN209890623U - Bioreactor aeration membrane effluent collecting device - Google Patents
Bioreactor aeration membrane effluent collecting device Download PDFInfo
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- CN209890623U CN209890623U CN201822014477.1U CN201822014477U CN209890623U CN 209890623 U CN209890623 U CN 209890623U CN 201822014477 U CN201822014477 U CN 201822014477U CN 209890623 U CN209890623 U CN 209890623U
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Abstract
The utility model relates to a bioreactor aeration membrane effluent collecting device, which comprises a bioreactor, a first liquid storage bottle, a first air filter, a second liquid storage bottle and a second air filter, wherein the top of the first liquid storage bottle is provided with an inlet, an air outlet and a water outlet, the first air filter is connected with the air outlet, and the aeration membrane outlet of the bioreactor is communicated with the inlet of the first liquid storage bottle; the water outlet is internally provided with a second pipeline, one end of the second pipeline extends to the bottom of the first liquid storage bottle, the top of the second liquid storage bottle is provided with an inlet and an outlet, the other end of the second pipeline is connected with a heat sealing pipe, the heat sealing pipe is connected with a third pipeline, the third pipeline is connected with the inlet of the second liquid storage bottle, and a second air filter is connected with the outlet of the second liquid storage bottle. The utility model can discharge the moisture in the ventilation membrane in production, avoid the blockage of the filter, prolong the service life of the air filter and reduce the replacement times; meanwhile, the contamination risk of cells in the culture process of the bioreactor can be reduced, so that the operation is safer.
Description
Technical Field
The utility model relates to a bioreactor technical field especially relates to bioreactor ventilates membrane and goes out water collection device.
Background
In the bioreactor, water is generated in the process of online sterilization and culture due to the characteristics of the used bubble-free aeration membrane, and the water is brought into an air filter at the outlet of the aeration membrane of the bioreactor when gas is introduced, so that the first air filter at the outlet is blocked, but if the water is directly discharged from a water outlet of the first air filter, the risk of bacterial contamination exists.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a bioreactor ventilates membrane and goes out water collection device can collect the play water of ventilating membrane exit, problem that can safe effectual processing air filter intake.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
bioreactor ventilates membrane play water collection device, including bioreactor, first stock solution bottle and first air filter, first stock solution bottle top is equipped with entry and gas outlet, first air filter is connected with the gas outlet, bioreactor's the membrane export of ventilating is through the entry intercommunication of first pipeline with first stock solution bottle.
Furthermore, a water outlet is arranged at the top of the first liquid storage bottle.
Further preferably, a second pipeline is arranged in the water outlet, and one end of the second pipeline extends to the bottom of the first liquid storage bottle.
Furthermore, the bioreactor aeration membrane effluent collecting device further comprises a second liquid storage bottle and a second air filter, an inlet and an outlet are formed in the top of the second liquid storage bottle, the other end of the second pipeline is connected with one end of a heat sealing pipe, the other end of the heat sealing pipe is connected with one end of a third pipeline, the other end of the third pipeline is connected with the inlet of the second liquid storage bottle, and the second air filter is connected with the outlet of the second liquid storage bottle.
Further, the first air filter is connected with the air outlet through a fourth pipeline, and the first pipeline, the second pipeline, the third pipeline and the fourth pipeline are silicone tubes.
Further, the bioreactor aeration membrane effluent collecting device further comprises a water stop clamp, and the water stop clamp clamps the second pipeline or the fourth pipeline. Or the water stop clamp clamps the third pipeline or the fourth pipeline.
Preferably, the second air filter is a butterfly air filter and the first air filter is a bladder air filter.
Preferably, the length of the heat-sealed tube is 15 cm.
Preferably, the volumes of the first liquid storage bottle and the second liquid storage bottle are both 1L, and the volume of the bioreactor is 30L.
Compared with the prior art, the utility model discloses following beneficial effect has:
the utility model can discharge the moisture in the ventilation membrane in production, avoid the blockage of the filter, prolong the service life of the air filter and reduce the replacement times; meanwhile, the contamination risk of cells in the culture process of the bioreactor can be reduced, so that the operation is safer.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
in the figure: 1-bioreactor, 2-first liquid storage bottle, 3-first air filter, 4-first pipeline, 5-second liquid storage bottle, 6-second air filter, 7-heat sealing pipe, 8-second pipeline, 9-third pipeline and 10-fourth pipeline.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the utility model discloses a bioreactor aeration membrane goes out water collection device, including bioreactor 1, first stock solution bottle 2, first air cleaner 3, second stock solution bottle 5 and second air cleaner 6, first stock solution bottle 2 top is equipped with entry, gas outlet and outlet, and first air cleaner 3 is connected with the gas outlet through fourth pipeline 10, and bioreactor 1's aeration membrane export is through the entry intercommunication of first pipeline 4 with first stock solution bottle 2.
A second pipeline 8 is arranged in the water outlet of the first liquid storage bottle 2, and one end of the second pipeline 8 extends to the bottom of the first liquid storage bottle 2. The top of the second liquid storage bottle 5 is provided with an inlet and an outlet, the other end of the second pipeline 8 is connected with one end of a heat sealing pipe 7, the other end of the heat sealing pipe 7 is connected with one end of a third pipeline 9, the other end of the third pipeline 9 is connected with the inlet of the second liquid storage bottle 5, and the second air filter 6 is connected with the outlet of the second liquid storage bottle 5 through a silicone tube. Preferably, the second air filter 6 is a butterfly air filter and the first air filter 3 is a bladder air filter.
The first pipeline 4, the second pipeline 8, the third pipeline 9 and the fourth pipeline 10 are silicone tubes. The length of heat-seal pipe 7 to and the volume of first stock solution bottle 2 and second stock solution bottle 5 sets up as required, and the length of heat-seal pipe 7 is 15cm in this embodiment, and the volume of first stock solution bottle 2 and second stock solution bottle 5 is 1L, and the volume of bioreactor 1 is 30L.
When the water in the first liquid storage bottle 2 is not discharged, the second pipeline 8 or the third pipeline 9 is clamped by a water stop clamp or a hemostatic forceps, at the moment, the water from the vent membrane outlet of the bioreactor 1 is collected in the first liquid storage bottle 2, and the gas is discharged after being filtered by the first air filter 3; when water in the first liquid storage bottle 2 is discharged, the water stop clamp or the hemostatic forceps on the second pipeline 8 or the third pipeline 9 are loosened, the fourth pipeline 10 is clamped by the water stop clamp or the hemostatic forceps, and the pressure in the first liquid storage bottle 2 is gradually increased due to the fact that gas is choked in the first liquid storage bottle 2, so that the water in the first liquid storage bottle 2 is discharged into the second liquid storage bottle 5 through the second pipeline 8, the heat sealing pipe 7 and the third pipeline 9. When the water in the second liquid storage bottle 5 is full, the heat sealing pipe 7 can be cut off by using a pipe sealing machine, and the water in the second liquid storage bottle 5 is transferred in the biological safety cabinet, so that the hidden trouble caused by the water outlet in the ventilation film is eliminated.
The utility model discloses simple structure, low cost, area is little, suits with the field layout of existing equipment. Meanwhile, the water in the ventilation membrane in production can be discharged, the filter is prevented from being blocked, the service life of the air filter is prolonged, and the replacement times are reduced; and the contamination risk of the cells in the culture process of the bioreactor can be reduced, so that the operation is safer.
Of course, the present invention can be embodied in many other forms without departing from the spirit or essential attributes thereof, and it should be understood that various changes and modifications can be made by one skilled in the art without departing from the spirit or essential attributes thereof, and that such changes and modifications are intended to be included within the scope of the appended claims.
Claims (10)
1. The bioreactor aeration membrane effluent collecting device is characterized by comprising a bioreactor, a first liquid storage bottle and a first air filter, wherein the top of the first liquid storage bottle is provided with an inlet and an air outlet, the first air filter is connected with the air outlet, and the aeration membrane outlet of the bioreactor is communicated with the inlet of the first liquid storage bottle through a first pipeline.
2. The bioreactor aeration membrane effluent collection device of claim 1, wherein: the top of the first liquid storage bottle is provided with a water outlet.
3. The bioreactor aeration membrane effluent collection device of claim 2, wherein: a second pipeline is arranged in the water outlet, and one end of the second pipeline extends to the bottom of the first liquid storage bottle.
4. The bioreactor aeration membrane effluent collection device of claim 3, wherein: the liquid storage device is characterized by further comprising a second liquid storage bottle and a second air filter, an inlet and an outlet are formed in the top of the second liquid storage bottle, the other end of the second pipeline is connected with one end of a heat sealing pipe, the other end of the heat sealing pipe is connected with one end of a third pipeline, the other end of the third pipeline is connected with the inlet of the second liquid storage bottle, and the second air filter is connected with the outlet of the second liquid storage bottle.
5. The bioreactor aeration membrane effluent collection device of claim 4, wherein: the first air filter is connected with the air outlet through a fourth pipeline, and the first pipeline, the second pipeline, the third pipeline and the fourth pipeline are silicone tubes.
6. The bioreactor aeration membrane effluent collection device of claim 5, wherein: still include the stagnant water clamp, the stagnant water clamp is cliied second pipeline or fourth pipeline.
7. The bioreactor aeration membrane effluent collection device of claim 5, wherein: still include the stagnant water clamp, the stagnant water clamp is cliied third pipeline or fourth pipeline.
8. The bioreactor aeration membrane effluent collection device of claim 4, wherein: the second air filter is a butterfly air filter, and the first air filter is a bag air filter.
9. The bioreactor aeration membrane effluent collection device of claim 4, wherein: the length of the heat-seal tube was 15 cm.
10. The bioreactor aeration membrane effluent collection device of claim 4, wherein: the volume of first stock solution bottle and second stock solution bottle is 1L, and bioreactor's volume is 30L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822014477.1U CN209890623U (en) | 2018-12-03 | 2018-12-03 | Bioreactor aeration membrane effluent collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822014477.1U CN209890623U (en) | 2018-12-03 | 2018-12-03 | Bioreactor aeration membrane effluent collecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209890623U true CN209890623U (en) | 2020-01-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822014477.1U Active CN209890623U (en) | 2018-12-03 | 2018-12-03 | Bioreactor aeration membrane effluent collecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209890623U (en) |
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2018
- 2018-12-03 CN CN201822014477.1U patent/CN209890623U/en active Active
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