CN201686685U - Automatic filling device for cell culture medium - Google Patents
Automatic filling device for cell culture medium Download PDFInfo
- Publication number
- CN201686685U CN201686685U CN 201020201647 CN201020201647U CN201686685U CN 201686685 U CN201686685 U CN 201686685U CN 201020201647 CN201020201647 CN 201020201647 CN 201020201647 U CN201020201647 U CN 201020201647U CN 201686685 U CN201686685 U CN 201686685U
- Authority
- CN
- China
- Prior art keywords
- substratum
- culture medium
- storage bottle
- filling device
- automatic filling
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The utility model provides an automatic filling device for a cell culture medium, comprising a gas tank, a culture medium storage bottle and a culture medium flow controlling system. The automatic filling device is characterized in that the gas tank is communicated with the culture medium storage bottle by a gas conduit; a pressure-reducing valve and a first electromagnetic valve are sequentially arranged on the gas conduit along the flowing direction of the gas; the culture medium storage bottle is provided with a culture medium conduit that is communicated with the culture medium storage bottle and used for discharging the culture medium; a second electromagnetic valve and a flow meter are arranged on the culture medium conduit along the flowing direction of the culture medium; and a filter is arranged at the outlet of the culture medium conduit. The automatic filling device comprises the gas tank for storing the pressure source, the culture medium storage bottle for temporarily storing the culture medium and the culture medium flow controlling system, reduces the working intensity of the operator, and also improves the culture medium filling efficiency.
Description
Technical field
The utility model relates to a kind of cell culture medium automatic filling device, in particular, relates in particular to a kind of nitrogen that utilizes as pressure source, the cell culture medium automatic filling device that is provided with under meter and can carries out continuous filtration.
Background technology
Entered since 21st century, the vital role of cell therapy in clinical treatment is bright and clear day by day, and stem cell particularly is because of it has the desirable seed cell that self, multidirectional differentiation potential more are regarded as cell therapy and organizational project.For example mesenchymal stem cells MSCs and umbilical cord mesenchymal stem cells can be used for treating autoimmune disorder and regenerative medicine, yet, source of human stem cell is limited, cultivate complicated operation, growing environment changes slightly will influence culture effect, and Application of stem cells is to carry out infusion according to patient's kg body weight, needs a large amount of amplification cultivation just can reach quantitative requirement.In the practical application of cell cultures,,, make the filling of substratum become a heavy work owing to now also do not have the higher adding set of automatization for a large amount of Tissue Culture Flasks substratum of annotating respectively is a routine job.Existing electronic suction assisting device all needs the suction pipe cell cultures bottleneck of coming in and going out repeatedly, and the substratum of annotating stage by stage must increase the microbial contamination probability.Though can also adopt needle-based filter filling substratum to reduce opportunities for contamination, when a large amount of culturing cell, it is too tired that push-and-pull syringe bolt can make operator's hand repeatedly.On the market a kind of auto-programming liquid changing device that cell cultures can be provided is arranged now, what it can be used for culture dish moves liquid certainly, but this device not only costs an arm and a leg, and the handiness deficiency, can not adapt to various kinds of cell culturing bottle and middle-size and small-size laboratory.
Summary of the invention
The utility model is in order to overcome the shortcoming of above-mentioned technical problem, provides a kind of nitrogen that utilizes as pressure source, the cell culture medium automatic filling device that is provided with under meter and can carries out continuous filtration.
Cell culture medium automatic filling device of the present utility model, comprise gas tank, substratum storage bottle and medium flow amount control system, its special feature is: described gas tank is connected with the substratum storage bottle by gas duct, and described gas duct upper edge gas flow direction is disposed with the reducing valve and first magnetic valve; Described substratum storage bottle is provided with the culture media conduit that substratum is discharged in coupled logical being used to, and this culture media conduit is disposed with second magnetic valve, under meter along the flow direction of substratum, is provided with strainer in the exit of culture media conduit; Described medium flow amount control system comprises first magnetic valve, second magnetic valve and the pilot circuit that is electrically connected with first magnetic valve and second magnetic valve.Gas tank is used to deposit the higher gas of pressure, and the substratum storage bottle is used for temporary substratum, in the process of using, should guarantee that the gas in the gas tank can not exert an influence to the substratum in the substratum storage bottle; Reducing valve is used for the gas of the gas duct of flowing through is carried out step-down, and first magnetic valve is used for the on off state of pilot-gas conduit; Culture media conduit is used for the cell culture medium of substratum storage bottle is transported in Tissue Culture Flask or other vessel, second magnetic valve on the culture media conduit is used to control the break-make of culture media conduit, under meter is used for the amount of effusive substratum is shown, so that operator quantitatively add substratum; Strainer is used to realize the filtration to substratum.Pilot circuit is controlled first magnetic valve and second magnetic valve, and first magnetic valve and second magnetic valve are in closing condition under cold situation, and promptly gas duct and culture media conduit are in off condition.
Gas in the cell culture medium automatic filling device of the present utility model, described gas tank is nitrogen, and described gas tank is provided with the gas tank valve that can carry out opening and closing to gas tank.Adopt nitrogen as pressure source, the one because the chemical property of nitrogen is very stable, substantially not with the substratum storage bottle in substratum react, the 2nd because the nitrogen low price obtains easily.
Cell culture medium automatic filling device of the present utility model also is provided with the substratum that is used for to substratum storage bottle filling substratum and adds pipe on the described substratum storage bottle, the top that this substratum adds pipe is provided with the interpolation tube valve.Because the substratum storage bottle just is used for keeping in substratum, in the process of using, the substratum in the substratum storage bottle need add or be replaced with other kind, can add pipe by substratum and realize; Be arranged on substratum and add the sterile state that the interpolation tube valve of managing the top can guarantee substratum.
Cell culture medium automatic filling device of the present utility model, described medium flow amount control system also comprises the push-switch that is used to control the pilot circuit break-make.First magnetic valve and second magnetic valve are controlled via pilot circuit by push-switch, made operator's both hands free, not only reduced labour intensity, and be convenient to change the Tissue Culture Flask operation.
Cell culture medium automatic filling device of the present utility model, the aperture of described strainer are 0.22 μ m, and the culture media conduit of strainer between the culture media conduit outlet is provided with the semisphere cover of fungi-proofing effect.In the process of Tissue Culture Flask the inside filling substratum, the semisphere cover be positioned at the Tissue Culture Flask bottleneck around, can effectively prevent the pollution of mushroom, the sterile state of useful maintenance substratum storage bottle to culturing bottle.
Cell culture medium automatic filling device of the present utility model, described flow is counted digital under meter.The benefit that adopts digital under meter is to be convenient to the control that the operator carries out flow observation and flow, and the amount that can guarantee to add substratum also can be increased work efficiency.
Cell culture medium automatic filling device of the present utility model, the outlet of described gas duct is positioned at the top of substratum storage bottle; The inlet of described culture media conduit is positioned at the bottom of substratum storage bottle.Substratum in the substratum storage bottle is to discharge by the increase of substratum storage bottle upper gas volume, and the bottom that the inlet of culture media conduit is arranged at the substratum storage bottle can increase the output of substratum.
The beneficial effects of the utility model are: (1) the utility model is provided with the gas tank in pressure store source, the substratum storage bottle and the medium flow amount control system of temporary substratum, not only reduced operator's working strength, but also the efficient of very high filling substratum can be carried out the substratum controllable screening for cell and tissue engineering product at short notice; (2) by strainer is set on culture media conduit, realized annotating and filtering and finish at the same time, not only improve working efficiency, and reduced algorithm, reduced the contaminated probability of substratum; (3) structure of the present utility model and control method are simple, and be with low cost and be easy to make.
Description of drawings
Fig. 1 is a theory structure synoptic diagram of the present utility model;
Among the figure: 1 gas tank, 2 gas tank valves, 3 reducing valve, 4 first magnetic valves, 5 gas ducts, 6 substratum storage bottle, 7 storage bottle sealing covers, 8 culture media conduit, 9 substratum add pipe, 10 add tube valve, 11 second magnetic valves, 12 under meters, 13 strainers, 14 semisphere covers, 15 pilot circuits, 16 push-switchs, 17 Tissue Culture Flasks.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing and embodiment.
As shown in Figure 1, cell culture medium automatic filling device of the present utility model comprises that gas tank 1, gas tank valve 2, reducing valve 3, first magnetic valve 4, gas duct 5, substratum storage bottle 6, storage bottle sealing cover 7, culture media conduit 8, substratum add pipe 9, add tube valve 10, second magnetic valve 11, under meter 12, strainer 13, semisphere cover 14, pilot circuit 15, push-switch 16, and the Tissue Culture Flask 17 shown in the figure is for holding the device of substratum.Gas in the gas tank 1 is nitrogen, gas tank valve 2 on the gas tank 1 can close and open gas tank 1, the output port of gas tank 1 is connected with the input port of gas duct 5, so that be implemented in when gas tank valve 2, reducing valve 3 and first magnetic valve 4 all are in open mode, gas tank 1 is a connected state with substratum storage bottle 6; Gas duct 5 upper edge gas flow directions are disposed with the reducing valve 3 and first magnetic valve 4, reducing valve 3 is used for the gas of gas tank 1 is reduced pressure, first magnetic valve 4 is realized the control of gas duct 5 break-makes, first magnetic valve 4 is electrically connected with pilot circuit 15, and first magnetic valve 4 is off condition under situation about not powering on.The output port of first magnetic valve 4 can be connected by withstand voltage flexible pipe with substratum storage bottle 6.
The flow direction of culture media conduit 8 upper edge substratum is disposed with second magnetic valve 11, under meter 12 and strainer 13, also is provided with semisphere cover 14 below strainer 13.By on-off control to second magnetic valve 11, can realize that substratum adds the conducting of pipe 9 and ends, second magnetic valve 11 is off condition under situation about not powering on.Under meter 12 is digital under meter, and the benefit that digital under meter is set is to be convenient to the quantity that the operator reads effusive substratum, is convenient to the amount of the substratum that added is controlled.The aperture of strainer 13 is 0.22 μ m, has realized the filtering function to substratum, and the semisphere cover 14 that is arranged on the strainer below can effectively prevent mushroom contamination of cells culturing bottle 17, has effectively guaranteed the sterile state of Tissue Culture Flask 17 bottlenecks.
First magnetic valve 4, second magnetic valve 11, pilot circuit 15, push-switch 16 have been formed the medium flow amount control system, the pilot circuit 15 and first magnetic valve 4 and second magnetic valve 11 are electrically connected, 16 pairs of pilot circuits 15 of push-switch are controlled, and then control the conducting and the off condition of first magnetic valve 4 and second magnetic valve 11.
The utility model is at first opened and is added tube valve 10 in the process of using, and substratum is added pipe 9 by substratum be filled in the substratum storage bottle 6, closes and adds tube valve 10; Open the reducing valve 3 on gas tank valve 2 and the gas duct 5 then successively; Then Tissue Culture Flask 17 is placed the below of semisphere cover 14, depress push-switch 16, first magnetic valve 4 and second magnetic valve 11 are opened simultaneously, make nitrogen be pressed into substratum storage bottle 6, medium flow in the substratum storage bottle 6 is flowed in the Tissue Culture Flask 17 after under meter 12 and the process filtration of strainer 13, the operator observes the volume of the substratum that injects Tissue Culture Flask 17 by under meter 12, when reaching the volume of regulation, lift push-switch 16, first magnetic valve 4 and second magnetic valve 11 are closed simultaneously, remove the Tissue Culture Flask 17 and the sealing of the substratum of annotating, prepare in next Tissue Culture Flask, to annotate substratum.Before the operation earlier with ultraviolet lamp to the Working environment sterilization and disinfection, and add pipe 9 filling sterile distilled water in substratum storage bottle 6 by substratum and wash whole piping system to disappear.
Claims (7)
1. cell culture medium automatic filling device, comprise gas tank (1), substratum storage bottle (6) and medium flow amount control system, it is characterized in that: described gas tank (1) is connected with substratum storage bottle (6) by gas duct (5), and described gas duct (5) upper edge gas flow direction is disposed with reducing valve (3) and first magnetic valve (4); Described substratum storage bottle (6) is provided with the culture media conduit (8) that substratum is discharged in coupled logical being used to, this culture media conduit (8) is disposed with second magnetic valve (11), under meter (12) along the flow direction of substratum, is provided with strainer (13) in the exit of culture media conduit (8); Described medium flow amount control system comprises first magnetic valve (4), second magnetic valve (11) and the pilot circuit (15) that is electrically connected with first magnetic valve and second magnetic valve.
2. cell culture medium automatic filling device according to claim 1 is characterized in that: the gas in the described gas tank (1) is nitrogen, and described gas tank (1) is provided with the gas tank valve (2) that can carry out opening and closing to gas tank (1).
3. cell culture medium automatic filling device according to claim 1 and 2, it is characterized in that: also be provided with the substratum that is used for to substratum storage bottle (6) filling substratum on the described substratum storage bottle (6) and add pipe (9), the top of this substratum interpolation pipe (9) is provided with adds tube valve (10).
4. cell culture medium automatic filling device according to claim 1 and 2 is characterized in that: described medium flow amount control system also comprises the push-switch (16) that is used to control pilot circuit (15) break-make.
5. cell culture medium automatic filling device according to claim 1 and 2, it is characterized in that: the aperture of described strainer (13) is 0.22 μ m, and the culture media conduit (8) of strainer (13) between culture media conduit (8) outlet is provided with the semisphere cover (14) of fungi-proofing effect.
6. cell culture medium automatic filling device according to claim 1 and 2 is characterized in that: described under meter (12) is digital under meter.
7. cell culture medium automatic filling device according to claim 1 and 2 is characterized in that: the outlet of described gas duct is positioned at the top of substratum storage bottle (6); The inlet of described culture media conduit (8) is positioned at the bottom of substratum storage bottle (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020201647 CN201686685U (en) | 2010-05-25 | 2010-05-25 | Automatic filling device for cell culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020201647 CN201686685U (en) | 2010-05-25 | 2010-05-25 | Automatic filling device for cell culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201686685U true CN201686685U (en) | 2010-12-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN 201020201647 Expired - Fee Related CN201686685U (en) | 2010-05-25 | 2010-05-25 | Automatic filling device for cell culture medium |
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| CN (1) | CN201686685U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN103828636A (en) * | 2012-11-20 | 2014-06-04 | 绿盈国际花卉有限公司 | Filling device of plant culture medium and operation method of filling device |
| CN105392876A (en) * | 2013-06-24 | 2016-03-09 | 威尔逊沃夫制造公司 | Closed system device and methods for gas permeable cell culture process |
| CN105675864A (en) * | 2016-02-29 | 2016-06-15 | 董玉俊 | Automatic bacteria sorting and marking device based on immune method |
| CN111433344A (en) * | 2017-11-30 | 2020-07-17 | 康宁股份有限公司 | Method and apparatus for aseptic dry transfer |
-
2010
- 2010-05-25 CN CN 201020201647 patent/CN201686685U/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN102174395B (en) * | 2011-01-30 | 2013-08-21 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN103828636A (en) * | 2012-11-20 | 2014-06-04 | 绿盈国际花卉有限公司 | Filling device of plant culture medium and operation method of filling device |
| CN103828636B (en) * | 2012-11-20 | 2016-01-27 | 绿盈国际花卉有限公司 | The device for filling of plant culture and method of operating thereof |
| EP3004320A4 (en) * | 2013-06-24 | 2017-05-17 | Wilson Wolf Manufacturing Corporation | Closed system device and methods for gas permeable cell culture process |
| CN105392876A (en) * | 2013-06-24 | 2016-03-09 | 威尔逊沃夫制造公司 | Closed system device and methods for gas permeable cell culture process |
| US9840692B2 (en) | 2013-06-24 | 2017-12-12 | Wilson Wolf Manufacturing | Closed system device and methods for gas permeable cell culture process |
| CN105392876B (en) * | 2013-06-24 | 2019-07-23 | 威尔逊沃夫制造公司 | Closed system apparatus and method for gas permeability cell cultivation process |
| CN110564612A (en) * | 2013-06-24 | 2019-12-13 | 威尔逊沃夫制造公司 | closed system devices and methods for gas permeable cell culture processes |
| US11155778B2 (en) | 2013-06-24 | 2021-10-26 | Wilson Wolf Manufacturing | Closed system device and methods for gas permeable cell culture process |
| US11873474B2 (en) | 2013-06-24 | 2024-01-16 | Wilson Wolf Manufacturing | Closed system device and methods for gas permeable cell culture process |
| CN105675864A (en) * | 2016-02-29 | 2016-06-15 | 董玉俊 | Automatic bacteria sorting and marking device based on immune method |
| CN105675864B (en) * | 2016-02-29 | 2017-11-07 | 董玉俊 | A kind of bacterium automatic sorting labelling apparatus based on immunization method |
| CN111433344A (en) * | 2017-11-30 | 2020-07-17 | 康宁股份有限公司 | Method and apparatus for aseptic dry transfer |
| US11655440B2 (en) | 2017-11-30 | 2023-05-23 | Corning Incorporated | Methods and devices for aseptic dry transfer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101229 Termination date: 20120525 |