CN105675864B - A kind of bacterium automatic sorting labelling apparatus based on immunization method - Google Patents
A kind of bacterium automatic sorting labelling apparatus based on immunization method Download PDFInfo
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- CN105675864B CN105675864B CN201610116775.4A CN201610116775A CN105675864B CN 105675864 B CN105675864 B CN 105675864B CN 201610116775 A CN201610116775 A CN 201610116775A CN 105675864 B CN105675864 B CN 105675864B
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- tank
- culture
- culture tank
- liquid pump
- conduit
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
Abstract
The invention discloses a kind of bacterium automatic sorting labelling apparatus based on immunization method, including substrate, the surface of the substrate is respectively equipped with magnetic bead fluid tank and fluorescent material tank, the magnetic bead fluid tank is connected with the first liquid pump by conduit, first liquid pump connects the first culture tank by conduit, the top of first culture tank is provided with the first injection port, and the fluorescent material tank connects the second liquid pump by conduit.The bacterium automatic sorting labelling apparatus based on immunization method, the culture bacterium amount for controlling the first culture tank and the second culture tank to be injected to the 3rd culture tank respectively by controller, quantitatively accurate culture can be realized, magnetic bead purpose bacterium complex is adsorbed by electromagnet, effect is good, and the 3rd culture tank and waste liquid tank, cleaning pipe are directly connected to, are conducive to improving cleaning purification efficiency, the purification used time is few, high financial profit.
Description
Technical field
The present invention relates to biological technical field, specially a kind of bacterium automatic sorting labelling apparatus based on immunization method.
Background technology
With continuing to develop for life science, biology of bacteria and amynologic basis research and clinical disease diagnosis with
Treatment is required for separation and purification of bacterial from the mixing sample containing different bacterium.Existing bacterium separation technology mainly has
Ficoll density gradient centrifugations, centrifugation elution, aqueous two-phase separation etc., these methods are all to utilize bacterium size, density, surface electricity
The feature of the physical properties such as lotus is separated, it is difficult to obtain ideal purity and the rate of recovery.
Cell sorting based on magnetic bead is the cell separation technology developed rapidly in recent years, first on magnetic bead
Labelled antibody molecule, specifically binds itself and cell, then by the effect of external magnetic field, sends out the cell combined with magnetic bead
Raw displacement and the separation for realizing aim cell.The method operation can obtain higher purity.But operated in incubation numerous
Trivial, it is impossible to realize quantitative accurate culture, purification process is longer, and purification efficiency is relatively low, therefore, it is proposed that a kind of based on immune side
The bacterium automatic sorting labelling apparatus of method.
The content of the invention
It is above-mentioned to solve it is an object of the invention to provide a kind of bacterium automatic sorting labelling apparatus based on immunization method
The problem of being proposed in background technology.
To achieve the above object, the present invention provides following technical scheme：A kind of bacterium automatic sorting based on immunization method
Labelling apparatus, including substrate, the surface of the substrate are respectively equipped with magnetic bead fluid tank and fluorescent material tank, the magnetic bead fluid tank
First liquid pump is connected with by conduit, first liquid pump connects the first culture tank, the top of first culture tank by conduit
End is provided with the first injection port, and the fluorescent material tank connects the second liquid pump by conduit, and second liquid pump is connected by conduit
Second culture tank, the top surface of second culture tank is provided with the second injection port, the bottom of first culture tank and the second culture tank
Substrate is run through at end, and the bottom of the first culture tank connects the first magnetic valve by conduit, and first magnetic valve passes through conduit
The 3rd culture tank is connected, the bottom of second culture tank connects the second magnetic valve by conduit, and second magnetic valve passes through
Conduit connects the 3rd culture tank, and the top of the 3rd culture tank is provided with electromagnet, and the bottom of the 3rd culture tank is provided with row
Outlet, and the bottom of the 3rd culture tank connects the 3rd liquid pump and the 4th liquid pump by conduit respectively, the 3rd liquid pump is by leading
Pipe connects purge tank, and the 4th liquid pump is connected in waste liquid tank, the 3rd culture tank, purge tank and waste liquid tank by conduit
Portion side is socketed with fixed cover, and the fixed cover upper surface is fixedly connected by fix bar with the bottom surface of substrate, the electromagnetism
Iron, the first liquid pump, the second liquid pump, the 3rd liquid pump, the 4th liquid pump, the first magnetic valve, the second magnetic valve are with being located at substrate surface
Controller electrical connection.
It is preferred that, the bottom of first culture tank and the second culture tank is infundibulate.
It is preferred that, catheter interior between first culture tank and the first magnetic valve is provided with first flowmeter, and described the
Flow meters electrically connect controller.
It is preferred that, catheter interior between second culture tank and the second magnetic valve is provided with second flowmeter, and described the
Two flowmeters electrically connect controller.
It is preferred that, the 3rd culture tank includes outer tank body and inner tank body, and the inner tank body is located in outer tank body, and outer tank
Space is left between body and inner tank body, spiral heater strip is provided with space, the heater strip electrically connects controller.
Compared with prior art, the beneficial effects of the invention are as follows：The bacterium automatic sorting mark dress based on immunization method
Put, the culture bacterium amount for controlling the first culture tank and the second culture tank to be injected to the 3rd culture tank respectively by controller, Ke Yishi
Now quantitative accurate culture, magnetic bead-purpose bacterium complex is adsorbed by electromagnet, and effect is good, by the 3rd culture tank and waste liquid tank,
Cleaning pipe is directly connected to, and is conducive to improving cleaning purification efficiency, the purification used time is few, high financial profit.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is the 3rd culture tank structural representation of the invention.
In figure：1 substrate, 2 fluorescent material tanks, 3 magnetic bead fluid tanks, 4 first culture tanks, 5 first injection ports, 6 first liquid pumps,
7 second liquid pumps, 8 second injection ports, 9 second culture tanks, 10 first magnetic valves, 11 second magnetic valves, 12 purge tanks, 13 the 3rd liquid
Pump, 14 outlets, 15 the 3rd culture tanks, 16 the 4th liquid pumps, 17 fixed covers, 18 waste liquid tanks, 19 fix bars, 20 heater strips, outside 151
Tank body, 152 inner tank bodys.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1-2 is referred to, the present invention provides a kind of technical scheme：A kind of bacterium automatic sorting mark based on immunization method
Device, including substrate 1, the surface of substrate 1 are respectively equipped with magnetic bead fluid tank 3 and fluorescent material tank 2, and magnetic bead fluid tank 3 is by leading
Pipe is connected with the first liquid pump 6, and the first liquid pump 6 connects the first culture tank 4 by conduit, can be by magnetic bead stream by the first liquid pump 6
Body is injected into the first culture tank 4, and the top of the first culture tank 4 is provided with the first injection port 5, can be injected purpose bacterium and be carried out magnetic
The culture of pearl composite bacterial, fluorescent material tank 2 connects the second liquid pump 7 by conduit, and the second liquid pump 7 is trained by conduit connection second
Tank 9 is supported, fluorescent material can be injected into the second culture tank 9 by the second liquid pump 7, the top surface of the second culture tank 9 is provided with the second note
Loophole 8, can inject the culture that purpose bacterium carries out fluorescent material composite bacterial, the He of the first culture tank 4 into the second culture tank 9
The bottom of second culture tank 9 is infundibulate, and the convenient nutrient solution by culture tank is all discharged, the first culture tank 4 and second
Substrate 1 is run through in the bottom of culture tank 9, and the bottom of the first culture tank 4 connects the first magnetic valve 10, the first culture by conduit
Catheter interior between the magnetic valve 10 of tank 4 and first is provided with first flowmeter, can be electrically connected with accurate flow control, first flowmeter
Connect controller, the first magnetic valve 10 connects the 3rd culture tank 15 by conduit, the bottom of the second culture tank 9 passes through conduit connection the
Two magnetic valves 11, the catheter interior between the second culture tank 9 and the second magnetic valve 11 is provided with second flowmeter, can accurately control
The Culture liquid measure of the discharge of second culture tank 9, second flowmeter electrical connection controller, the second magnetic valve 11 passes through conduit connection the
Three culture tanks 15, the top of the 3rd culture tank 15 is provided with electromagnet, and the bottom of the 3rd culture tank 15 is provided with outlet 14, and the 3rd
The bottom of culture tank 15 connects the 3rd liquid pump 13 and the 4th liquid pump 16 by conduit respectively, and the 3rd liquid pump 13 connects clear by conduit
Cleaning of evaporator 12, the 4th liquid pump 16 connects waste liquid tank 18, the medial side of the 3rd culture tank 15, purge tank 12 and waste liquid tank 18 by conduit
Face is socketed with fixed cover 17, and the upper surface of fixed cover 17 is fixedly connected by fix bar 19 with the bottom surface of substrate 1, electromagnet,
One liquid pump 6, the second liquid pump 7, the 3rd liquid pump 13, the 4th liquid pump 16, the first magnetic valve 10, the second magnetic valve 11 are with being located at substrate
The controller electrical connection on 1 surface, the 3rd culture tank 15 includes outer tank body 151 and inner tank body 152, and inner tank body 152 is located at outer tank body
In 151, and space is left between outer tank body 151 and inner tank body 152, spiral heater strip 20, heater strip are provided with space
20 electrical connection controllers, magnetic bead composite bacterial and fluorescent material composite bacterial are cultivated in the 3rd culture tank 15, pass through heater strip
20 can ensure cultivation temperature, so as to obtain magnetic bead-fluorescent material composite bacterial, purge tank can be extracted by the 3rd liquid pump 13
Cleaning fluid in 12 cleans cultured composite bacterial, and then opening electromagnet by controller adsorbs composite bacterial the 3rd
The top of culture tank 15, cleans the waste liquid formed and is entered by the 4th liquid pump 16 in waste liquid tank 18, process completion is repeated
Purification to composite bacterial, is somebody's turn to do the bacterium automatic sorting labelling apparatus based on immunization method, first is controlled respectively by controller
The culture bacterium amount that the culture tank 9 of culture tank 4 and second is injected to the 3rd culture tank 15, it is possible to achieve quantitative accurate culture, passes through electricity
Magnet adsorption magnetic bead-purpose bacterium complex, effect is good, and the 3rd culture tank 15 and waste liquid tank 18, cleaning pipe 12 are directly connected to,
Be conducive to improving cleaning purification efficiency, the purification used time is few, high financial profit.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of bacterium automatic sorting labelling apparatus based on immunization method, including substrate (1), it is characterised in that：The substrate
(1) surface is respectively equipped with magnetic bead fluid tank (3) and fluorescent material tank (2), and the magnetic bead fluid tank (3) is connected with by conduit
First liquid pump (6), first liquid pump (6) connects the first culture tank (4), the top of first culture tank (4) by conduit
Provided with the first injection port (5), the fluorescent material tank (2) connects the second liquid pump (7) by conduit, and second liquid pump (7) is led to
Cross conduit and connect the second culture tank (9), the top surface of second culture tank (9) is provided with the second injection port (8), first culture
Substrate (1) is run through in the bottom of tank (4) and the second culture tank (9), and the bottom of the first culture tank (4) connects first by conduit
Magnetic valve (10), first magnetic valve (10) connects the 3rd culture tank (15), the bottom of second culture tank (9) by conduit
Portion connects the second magnetic valve (11) by conduit, and second magnetic valve (11) connects the 3rd culture tank (15), institute by conduit
The top for stating the 3rd culture tank (15) is provided with electromagnet, and the bottom of the 3rd culture tank (15) is provided with outlet (14), and the
The bottom of three culture tanks (15) connects the 3rd liquid pump (13) and the 4th liquid pump (16), the 3rd liquid pump (13) by conduit respectively
Purge tank (12) is connected by conduit, the 4th liquid pump (16) connects waste liquid tank (18), the 3rd culture tank by conduit
(15), the middle part side of purge tank (12) and waste liquid tank (18) is socketed with fixed cover (17), fixed cover (17) upper surface
It is fixedly connected by fix bar (19) with the bottom surface of substrate (1), the electromagnet, the first liquid pump (6), the second liquid pump (7), the 3rd
Liquid pump (13), the 4th liquid pump (16), the control of the first magnetic valve (10), the second magnetic valve (11) with being located at substrate (1) surface
Device is electrically connected.
2. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
The bottom for stating the first culture tank (4) and the second culture tank (9) is infundibulate.
3. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State the catheter interior between the first culture tank (4) and the first magnetic valve (10) and be provided with first flowmeter, the first flowmeter electricity
Connect controller.
4. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
State the catheter interior between the second culture tank (9) and the second magnetic valve (11) and be provided with second flowmeter, the second flowmeter electricity
Connect controller.
5. a kind of bacterium automatic sorting labelling apparatus based on immunization method according to claim 1, it is characterised in that：Institute
Stating the 3rd culture tank (15) includes outer tank body (151) and inner tank body (152), and the inner tank body (152) is located at outer tank body (151)
In, and space is left between outer tank body (151) and inner tank body (152), and spiral heater strip (20) is provided with space, it is described
Heater strip (20) electrically connects controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610116775.4A CN105675864B (en) | 2016-02-29 | 2016-02-29 | A kind of bacterium automatic sorting labelling apparatus based on immunization method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610116775.4A CN105675864B (en) | 2016-02-29 | 2016-02-29 | A kind of bacterium automatic sorting labelling apparatus based on immunization method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105675864A CN105675864A (en) | 2016-06-15 |
| CN105675864B true CN105675864B (en) | 2017-11-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610116775.4A Expired - Fee Related CN105675864B (en) | 2016-02-29 | 2016-02-29 | A kind of bacterium automatic sorting labelling apparatus based on immunization method |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108414752A (en) * | 2018-03-28 | 2018-08-17 | 黄河科技学院 | A kind of bacterium automatic sorting labeling method and device based on immunization method |
| DE102019134003A1 (en) | 2019-12-11 | 2021-06-17 | Aixinno Limited | Method and device for culturing biological cells |
| CN113583934A (en) * | 2021-07-30 | 2021-11-02 | 阿谷巴(杭州)生物科技发展有限公司 | Culture medium for rapidly detecting bacteria in cell product and preparation method and application thereof |
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| CN202688239U (en) * | 2012-07-20 | 2013-01-23 | 上海理工大学 | Extraction device for monoclonal antibody preparation |
| WO2014054086A1 (en) * | 2012-10-05 | 2014-04-10 | 株式会社日進製作所 | Fluid purification device |
| CN104946528A (en) * | 2015-07-16 | 2015-09-30 | 中南大学湘雅医院 | Primary hepatic tissue cell separation system and method |
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2016
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999034848A1 (en) * | 1998-01-08 | 1999-07-15 | Nexell Therapeutics Inc. | Method, bag and disposable set for recirculation washing of blood cells |
| CN1263893A (en) * | 1999-02-05 | 2000-08-23 | 上海复旦张江生物医药有限公司 | Renaturation equipment of modified protein |
| CN201686685U (en) * | 2010-05-25 | 2010-12-29 | 时庆 | Automatic filling device for cell culture medium |
| CN102504991A (en) * | 2011-10-11 | 2012-06-20 | 中国人民解放军军事医学科学院卫生装备研究所 | Device and method for non-specific rapid enrichment and purification of bacteria in liquid sample |
| CN202688239U (en) * | 2012-07-20 | 2013-01-23 | 上海理工大学 | Extraction device for monoclonal antibody preparation |
| WO2014054086A1 (en) * | 2012-10-05 | 2014-04-10 | 株式会社日進製作所 | Fluid purification device |
| CN104946528A (en) * | 2015-07-16 | 2015-09-30 | 中南大学湘雅医院 | Primary hepatic tissue cell separation system and method |
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| CN105675864A (en) | 2016-06-15 |
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Effective date of registration: 20210111 Address after: Room 606, 6th floor, comprehensive building, community entrepreneurship Park, Langya Economic Development Zone, Chuzhou City, Anhui Province Patentee after: Anhui xinjiuya Photoelectric Technology Co.,Ltd. Address before: 252000 No.3, Dongguan Street, Dongchangfu District, Liaocheng City, Shandong Province Patentee before: Dong Yujun |
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