CN203575282U - Automatic sterilizer for tissue culture - Google Patents

Automatic sterilizer for tissue culture Download PDF

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Publication number
CN203575282U
CN203575282U CN201320626504.5U CN201320626504U CN203575282U CN 203575282 U CN203575282 U CN 203575282U CN 201320626504 U CN201320626504 U CN 201320626504U CN 203575282 U CN203575282 U CN 203575282U
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CN
China
Prior art keywords
sterilization
liquid
magnetic valve
automatic sterilizer
thimerosal storage
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201320626504.5U
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Chinese (zh)
Inventor
张琳
夏宜平
沈柏春
支建磊
佘琳芳
魏建芬
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HANGZHOU LANDSCAPING Inc
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HANGZHOU LANDSCAPING Inc
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Priority to CN201320626504.5U priority Critical patent/CN203575282U/en
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Abstract

The utility model relates to an automatic sterilizer for tissue culture. Operators need to shake sterilization canisters with hands to enable sterilization liquid to come into full contact with plant materials in traditional manual sterilization modes and such sterilization modes have low efficiency, the sterilization liquid comes into contact with experimenters and workers, thus being not beneficial for labor protection, and the manual operation process easily causes secondary pollution. The automatic sterilizer is characterized by comprising a plurality of sterilization liquid storage canisters, wherein each sterilization liquid storage canister is connected to a same explant sterilization liquid canister by a pipeline; a liquid conveying solenoid valve is arranged on each pipeline; the explant sterilization liquid canister is connected with a liquid drain pipe; a waste liquid drain solenoid valve is arranged on the liquid drain pipe; switches of the liquid conveying solenoid valve and the waste liquid drain solenoid valve are controlled by a chip; the chip is externally connected with a control panel. The automatic sterilizer achieves automatic control of different sterilization stage time, isolates experimental workers from the sterilization liquid, and is simple and safe to operate, and the work efficiency is effectively improved.

Description

A kind of tissue is cultivated automatic sterilizer
Technical field
The utility model has related to a kind of agriculture field and the applicable machine of laboratory plant tissue culture fast breeding technique, has particularly related to a kind of tissue and has cultivated automatic sterilizer.
Background technology
In agriculture field and laboratory experiment system, tissue culture quick breeding system and technology can rapid, high volume the numerous good plant of expansion system, production group training seedling or as other systems of laboratory as the basis of genetic conversion system, its crucial step is just generation tissue cultivation, from field material to tissue-culture container seedling, impact is just explant sterilization for the important step of organizing culture success ratio, fully effectively sterilization just can be avoided the bacterium under condition of tissue culture, fungal contamination, and Traditional Man disinfection efficiency is low, easily secondary pollution, how to realize sterilization automation, reducing labour cost is technical bottleneck.
The thimerosal that just culture is used mainly comprises mercuric chloride, clorox, alcohol, needs to control the disinfecting time of each thimerosal.If conventional lily bulb disinfectant program is 75% alcohol disinfecting 30s, 2%NaClO or mercuric chloride are processed 6-15min, aseptic water washing 5~6 times (each 30s), and each sterilization needs to change fresh fertilized liquid, does not stop to rock.Traditional artificial sterilization needs operating personnel manually to rock disinfection tank so that thimerosal fully contacts with vegetable material, and relies on stopwatch simultaneously and regularly change fresh fertilized liquid.This sterilization pattern efficiency is low, thimerosal easily contacts with experiment and staff, be unfavorable for labour protection, and manual operation process easily causes secondary pollution.
Summary of the invention
The utility model, for above-mentioned defect, provides a kind of tissue to cultivate automatic sterilizer, realizes the different sterilisation stage time of automatically controlling, easy-to-operate, and efficiency is high.
For this reason, the utility model is taked following technical scheme, a kind of tissue is cultivated automatic sterilizer, it is characterized in that comprising a plurality of thimerosal storage tanks, each thimerosal storage tank is connected to same explant disinfectant solution tank by pipeline respectively, and transfusion magnetic valve is all set on each pipeline, described explant disinfectant solution tank connected drainage pipe, removal waste fluid magnetic valve is set on discharging tube, and described transfusion magnetic valve and the switch of removal waste fluid magnetic valve be by chip controls, the external control panel of chip.User can set amount and the every kind of soak time that thimerosal enters explant disinfectant solution tank that sterilised liq flows into explant disinfectant solution tank by control panel.The control parameter that control panel is set user is input in control chip, control chip calculates the folding time of each magnetic valve, sends chronologically control instruction, thereby realizes explant automation sterilization, save experimenter's working time and working strength, increase work efficiency.
The upper cover sealing of described thimerosal storage tank, the sidepiece of thimerosal storage tank also arranges an air pressure balance magnetic valve, described air pressure balance magnetic valve and transfusion magnetic valve synchro switch.The sealing of thimerosal storage tank can effectively prevent that toxic gas from leaking, and increases processing safety, air pressure balance magnetic valve is set and can keeps tank internal gas pressure consistent with external atmosphere pressure.
Described thimerosal storage tank is three, stores respectively mercury, alcohol and distilled water in it, and three groups of magnetic valves on three described thimerosal storage tanks are sequentially opened by chip controls or be closed.Sequentially to inputting the object that different liquids reaches sterilization in explant disinfectant solution tank, automatically realize as required, save experimenter's working time and working strength.
The external waste liquid bottle of described discharging tube.
The utility model has been realized the different sterilisation stage time of automatic control, isolation experiment staff and thimerosal, and safety simple to operate, has effectively promoted operating efficiency.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model
Embodiment
Below in conjunction with specific embodiment, further set forth the utility model.
A kind of tissue is as shown in Figure 1 cultivated automatic sterilizer, comprise three thimerosal storage tanks 2, each thimerosal storage tank is connected to same explant disinfectant solution tank 5 by conduit 4 respectively, transfusion magnetic valve 3 is all set on each pipeline 4, explant disinfectant solution tank 5, by the external waste liquid bottle 8 of discharging tube 7, arranges removal waste fluid magnetic valve 6 on discharging tube 7.The upper cover sealing of each thimerosal storage tank, the sidepiece of thimerosal storage tank 2 also arranges an air pressure balance magnetic valve 1.Three interior mercury, alcohol and distilled water stored respectively of thimerosal storage tank 2.Transfusion magnetic valve 3, air pressure balance magnetic valve 1 and removal waste fluid magnetic valve 6 switch by chip controls, the external control panel of chip.Air pressure balance magnetic valve 1 and transfusion magnetic valve 3 synchro switches.Three groups of magnetic valves on three thimerosal storage tanks 2 are sequentially opened by chip controls or are closed.
The course of work is as follows: when needs thimerosal flows in explant disinfection tank, transfusion magnetic valve and air pressure balance magnetic valve are opened simultaneously, make thimerosal flow via a conduit explant disinfectant solution tank.The magnetic valve of mercury, alcohol, three fluid reservoirs of distilled water is sequentially opened, thimerosal flows in explant disinfectant solution tank, soak the some time, removal waste fluid magnetic valve is opened, discharge discarded thimerosal, then removal waste fluid closed electromagnetic valve, another kind of thimerosal starts to flow in explant disinfection tank explant sterilization simultaneously, and job step is the same similar thereafter.
The opening and closing of seven magnetic valves, are to control by control chip, and control chip has clocking capability, thereby can set the time that magnetic valve opens and closes.Thimerosal flows into the amount in disinfection tank, can set by control time that magnetic valve and air pressure balance magnetic valve open of infusing.And the time of medicining liquid dipping explant can set by the infuse length in the time interval that magnetic valve, air pressure balance magnetic valve and removal waste fluid magnetic valve open of control.
Equipment has control panel outward, and user can set three kinds of liquid by control panel and flow into the amount of explant and the time of three kinds of soaked with liquid explants.The control parameter that control panel is set user is input in control chip, control chip calculates the folding time of each magnetic valve, sends chronologically control instruction, thereby realizes explant automation sterilization, save experimenter's working time and working strength, increase work efficiency.
Should be understood that these embodiment are only not used in restriction scope of the present utility model for the utility model is described.In addition should be understood that those skilled in the art can make various changes or modifications the utility model after having read the content of the utility model instruction, these equivalent form of values fall within the application's appended claims limited range equally.

Claims (4)

1. a tissue is cultivated automatic sterilizer, it is characterized in that comprising a plurality of thimerosal storage tanks, each thimerosal storage tank is connected to same explant disinfectant solution tank by pipeline respectively, transfusion magnetic valve is all set on each pipeline, described explant disinfectant solution tank connected drainage pipe, removal waste fluid magnetic valve is set on discharging tube, and described transfusion magnetic valve and the switch of removal waste fluid magnetic valve be by chip controls, the external control panel of chip.
2. a kind of tissue according to claim 1 is cultivated automatic sterilizer, it is characterized in that the upper cover sealing of described thimerosal storage tank, the sidepiece of thimerosal storage tank also arranges an air pressure balance magnetic valve, described air pressure balance magnetic valve and transfusion magnetic valve synchro switch.
3. a kind of tissue according to claim 2 is cultivated automatic sterilizer, it is characterized in that described thimerosal storage tank is three, in it, store respectively mercury, alcohol and distilled water, three groups of magnetic valves on three described thimerosal storage tanks are sequentially opened by chip controls or are closed.
4. according to a kind of tissue described in claim 1 or 3, cultivate automatic sterilizer, it is characterized in that the external waste liquid bottle of described discharging tube.
CN201320626504.5U 2013-10-11 2013-10-11 Automatic sterilizer for tissue culture Expired - Fee Related CN203575282U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201320626504.5U CN203575282U (en) 2013-10-11 2013-10-11 Automatic sterilizer for tissue culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201320626504.5U CN203575282U (en) 2013-10-11 2013-10-11 Automatic sterilizer for tissue culture

Publications (1)

Publication Number Publication Date
CN203575282U true CN203575282U (en) 2014-05-07

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CN201320626504.5U Expired - Fee Related CN203575282U (en) 2013-10-11 2013-10-11 Automatic sterilizer for tissue culture

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112569723A (en) * 2020-12-09 2021-03-30 武汉市中卫寰宇医疗系统工程有限公司 Full-automatic medical treatment clean system
CN113455390A (en) * 2021-06-10 2021-10-01 江西环境工程职业学院 High-automatic plant tissue culture explant disinfection induction method and equipment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112569723A (en) * 2020-12-09 2021-03-30 武汉市中卫寰宇医疗系统工程有限公司 Full-automatic medical treatment clean system
CN113455390A (en) * 2021-06-10 2021-10-01 江西环境工程职业学院 High-automatic plant tissue culture explant disinfection induction method and equipment

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140507

Termination date: 20191011

CF01 Termination of patent right due to non-payment of annual fee