CN113455390A - High-automatic plant tissue culture explant disinfection induction method and equipment - Google Patents
High-automatic plant tissue culture explant disinfection induction method and equipment Download PDFInfo
- Publication number
- CN113455390A CN113455390A CN202110649676.3A CN202110649676A CN113455390A CN 113455390 A CN113455390 A CN 113455390A CN 202110649676 A CN202110649676 A CN 202110649676A CN 113455390 A CN113455390 A CN 113455390A
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- China
- Prior art keywords
- fixedly connected
- motor
- tissue culture
- support
- plant tissue
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 71
- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 54
- 230000006698 induction Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000005520 cutting process Methods 0.000 claims abstract description 31
- 238000011081 inoculation Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 41
- 230000001954 sterilising effect Effects 0.000 claims description 34
- XTEGARKTQYYJKE-UHFFFAOYSA-N chloric acid Chemical compound OCl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-N 0.000 claims description 23
- 229940005991 chloric acid Drugs 0.000 claims description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 19
- 238000001816 cooling Methods 0.000 claims description 19
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 15
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 14
- 229920000742 Cotton Polymers 0.000 claims description 13
- 238000003825 pressing Methods 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 230000011218 segmentation Effects 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 abstract description 14
- 239000008223 sterile water Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/003—Cutting apparatus specially adapted for tissue culture
Abstract
The invention relates to the technical field of plant seedling culture, in particular to a high-automatic disinfection induction method and equipment for explants for plant tissue culture, which comprises a bracket, wherein the left end of the bracket is fixedly connected with a controller, the inner side of the bottom end of the bracket is fixedly connected with a disinfection device, the left side of the disinfection device is provided with a cutting device, the lower side of the cutting device is provided with a transplanting device, the transplanting device is fixedly connected with the bracket, the left end of the bracket is fixedly connected with a fourth motor, the tail end of a main shaft of the fourth motor is fixedly connected with a second adjusting screw rod, the second adjusting screw rod is rotatably connected with the bracket, the outer side of the second adjusting screw rod is spirally connected with a second sliding block, the second sliding block is slidably connected with the bracket, and the disinfection of tissue culture seedlings of plants can be completed through the arranged disinfection device, the cutting device, the transplanting device, a clamp and an ultraviolet disinfection lamp, The cutting and inoculation integrated treatment does not need separate and independent treatment, thereby improving the working efficiency and reducing the working strength.
Description
Technical Field
The invention relates to the technical field of plant seedling culture, in particular to a high-automatic disinfection induction method and equipment for a plant tissue culture explant.
Background
The tissue culture of plants is called in vitro culture, and refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from plant bodies, and inoculating the isolated tissues, organs or cells, protoplasts, etc. to a culture medium containing various nutrients and plant hormones under aseptic conditions for culture to obtain regenerated whole plants or produce other products having economic value.
In some plant tissue culture processes, a series of operations are needed to ensure the survival rate of plant tissue culture, when the plant tissue culture is carried out, the plant needs to be cleaned and cut, the plant tissue culture is placed in a culture dish after the plant tissue culture is cut, and the conventional plant tissue culture explants are separately and independently carried out, so that the labor intensity of workers is increased, and the efficiency of the plant tissue culture is reduced.
Disclosure of Invention
The invention aims to provide a high-automatic plant tissue culture explant disinfection induction method and equipment, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a high-automatic plant tissue culture explant disinfection induction method and equipment comprise a support, wherein a controller is fixedly connected to the left end of the support, a disinfection device is fixedly connected to the inner side of the bottom end of the support, a cutting device is arranged on the left side of the disinfection device, a transplanting device is arranged on the lower side of the cutting device and is fixedly connected with the support, a fourth motor is fixedly connected to the left end of the support, a second adjusting screw is fixedly connected to the tail end of a main shaft of the fourth motor and is rotatably connected with the support, a second sliding block is spirally connected to the outer side of the second adjusting screw and is slidably connected with the support, a fourth electric telescopic rod is fixedly connected to the bottom end of the second sliding block, a fifth motor is fixedly connected to the bottom end of the fourth electric telescopic rod, and a connecting seat is fixedly connected to the tail end of the main shaft of the fifth motor, the ultraviolet disinfection lamp comprises a connecting seat, a first adjusting screw rod, a third slider, a vertical rod, a clamp and an ultraviolet disinfection lamp, wherein the first adjusting screw rod is connected with the connecting seat in a screwed mode, the first adjusting screw rod is fixedly connected with the tail end of a main shaft of the sixth motor, the first adjusting screw rod is rotatably connected with the connecting seat, the third slider is spirally connected with the outer side of the first adjusting screw rod, the vertical rod is fixedly connected with the bottom end of the third slider, and the ultraviolet disinfection lamp is fixedly connected with the two sides of the clamp.
Preferably, the disinfection device comprises a water storage tank, a cooling pipe, a storage rack, an alcohol barrel, a sodium hypochlorite barrel, a connecting pipe, a solenoid valve, a connecting plate, a water pump, a liquid outlet pipe, a fixed box, a slide rail, a first slide block, a first motor, an oil seal, a rotary disc, a sealing ring, a guide wheel, a support plate, a cam, a second motor, a connecting cylinder, a spring, a limiting block, an end cover, a connecting shaft and a disinfection box, wherein one end of the support is fixedly connected with the water storage tank, the inner side of the water storage tank is fixedly connected with the cooling pipe, the top end of the water storage tank is fixedly connected with the storage rack, the inner side of the storage rack is provided with the alcohol barrel and the sodium hypochlorite barrel, the cooling pipe, the alcohol barrel and the sodium hypochlorite barrel are all communicated with the connecting pipe through pipelines, the solenoid valves are arranged at outlets of the cooling pipe, the alcohol barrel and the sodium hypochlorite barrel, and the right end of the water storage tank is fixedly connected with the connecting plate, the top fixedly connected with water pump of connecting plate, the exit end intercommunication of water pump has the drain pipe, the exit end of drain pipe is provided with the disinfect box.
Preferably, one end of the bracket is fixedly connected with a fixed box, the inner side of the top end of the fixed box is fixedly connected with a slide rail, the outer side of the slide rail is slidably connected with a first slide block, the top end of the first slide block is fixedly connected with a disinfection box, the inner side of the right end of the fixed box is fixedly connected with a support plate, the rear end of the support plate is fixedly connected with a second motor, the tail end of a main shaft of the second motor is fixedly connected with a cam, one side of the cam is provided with a guide wheel which is fixedly connected with the disinfection box, the inner side of the left end of the fixed box is fixedly connected with a connecting cylinder, the inner side of the connecting cylinder is fixedly connected with a spring, the other end of the spring is fixedly connected with a limiting block, the limiting block is slidably connected with the connecting cylinder, the right end of the limiting block is fixedly connected with a connecting shaft which is fixedly connected with the disinfection box, and the outer side of the connecting shaft is slidably connected with an end cover, and the end cover is fixedly connected with the connecting cylinder.
Preferably, the first motor of bottom fixedly connected with of disinfect box, the terminal fixedly connected with carousel of main shaft of first motor, and the carousel rotates with the disinfect box to be connected, the outside fixedly connected with sealing ring of carousel, and the sealing ring rotates with the disinfect box to be connected, the transmission shaft outside of first motor rotates and is connected with the oil blanket, and oil blanket and disinfect box fixed connection.
Preferably, the cutting device comprises a belt conveyor, a first chloric acid brush, a second electric telescopic rod, a connecting frame, sterilized cotton, a third motor, a cutter, an infrared induction lamp, a third electric telescopic rod, a pressing block, a seventh motor, a rotary drum, an eighth motor, a lead screw, a movable block, a side plate, a placing frame, a clamping plate and a fourth electric telescopic rod, wherein one end of the support is fixedly connected with the belt conveyor, one end of the support is fixedly connected with the seventh motor, the tail end of a main shaft of the seventh motor is fixedly connected with the rotary drum, the inner side of the rotary drum is fixedly connected with the eighth motor, the tail end of a main shaft of the eighth motor is fixedly connected with the lead screw, the outer side of the lead screw is spirally connected with the movable block, one end of the movable block is fixedly connected with the side plate, one end of the side plate is fixedly connected with the placing frame, and the inner side of the placing frame is fixedly connected with the fifth electric telescopic rod, and the other end of the fifth electric telescopic rod is fixedly connected with a clamping plate.
Preferably, one end of the support is fixedly connected with a third electric telescopic rod, and the other end of the third electric telescopic rod is fixedly connected with a pressing block.
Preferably, the bottom of band conveyer is provided with the brush of chloric acid for the first time, and chloric acid for the first time brush and support fixed connection, the bottom of rotary drum is provided with the brush of chloric acid for the second time, and chloric acid for the second time brush and support fixed connection.
Preferably, the one end fixedly connected with second electric telescopic handle of support, the other end fixedly connected with carriage of second electric telescopic handle, the one end fixedly connected with third motor of carriage, the terminal fixedly connected with cutter of main shaft of third motor, the both sides of cutter are provided with the aseptic cotton, and aseptic cotton and carriage fixed connection, the bottom fixedly connected with infrared induction lamp of carriage.
Preferably, the transplanting device comprises a support column, a rotary table, a ninth motor, a press switch, a disc, a lug and an inoculation bottle, the ninth motor is fixedly connected with the inner side of the top end of the support column, the rotary table is fixedly connected with the end of the main shaft on the upper side of the ninth motor, the disc is fixedly connected with the end of the main shaft on the lower side of the ninth motor, the lug is fixedly connected with the outer side of the disc, a groove used for inserting a culture dish is formed in the inner side of the rotary table, and the number of the grooves in the rotary table is the same as the number of the lugs in the disc.
Preferably, the step one: firstly, disinfecting the plant tissue culture by a disinfection device;
step two: performing segmented cutting on the disinfected plant tissue culture by using a cutting device;
step three: and (4) inoculating the plant tissue culture after the segmentation and cutting through a transplanting device.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the integrated treatment of disinfection, cutting and inoculation of the plant tissue culture seedlings can be completed through the disinfection device, the cutting device, the transplanting device, the third slide block, the vertical rod, the clamp and the ultraviolet disinfection lamp, and the separate and independent treatment is not needed, so that the working efficiency is improved, and the working intensity is reduced.
2. According to the invention, through the arrangement of the water storage tank, the cooling pipe, the alcohol barrel, the sodium hypochlorite barrel, the water pump, the sliding rail, the first sliding block, the rotating disc, the cam, the spring, the connecting shaft and the disinfection box, the plant tissue culture seedlings can be disinfected through the disinfection box, the disinfection box can vibrate under the action of the cam during disinfection, the disinfection is carried out while vibrating, the disinfection efficiency and the disinfection effect are improved, manual vibration disinfection is not needed, and the labor intensity of workers is reduced.
3. According to the invention, through the belt conveyor, the first chloric acid brush, the second chloric acid brush, the sterilized cotton, the pressing block, the cutter, the infrared induction lamp, the seventh motor, the rotary drum, the moving block and the clamping plate, the plant tissue culture can be cut through the cutter, the cut plant tissue culture can be inserted into the inner side of the inoculation bottle under the action of the clamping plate, and meanwhile, the pressing block can ensure that the plant tissue culture cannot deviate in the cutting process.
4. According to the invention, through the arranged supporting columns, the rotating disc, the press switch, the disc, the convex blocks and the inoculation bottle, the cut plant rhizomes can continue to move rightwards under the action of the sliding plate, the transplanting device works, the fourth motor rotates to drive the rotating disc and the disc to rotate synchronously, when the convex blocks on the disc press the press switch, the fourth motor stops rotating, the culture dish on the rotating disc can be over against the right end of the plant rhizomes, and the plant rhizomes can enter the inner side of the culture dish after continuing to move rightwards, so that the transplanting of the plant rhizomes is realized.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is an enlarged view of the mounting structure of the inside of the fixing box according to the present invention;
FIG. 3 is an enlarged view of the installation structure of the cutting device of the present invention;
FIG. 4 is an enlarged view of the mounting structure of the cutter according to the present invention;
FIG. 5 is an enlarged view of the mounting structure of the clamping plate of the present invention;
FIG. 6 is a schematic top view of the mounting structure of the cleat of the present invention;
FIG. 7 is a schematic view of the structure of the cutter according to the present invention;
FIG. 8 is a schematic view of the structure at A of FIG. 3 according to the present invention.
In the figure: 1-disinfection device, 101-water storage tank, 102-cooling pipe, 103-storage rack, 104-alcohol barrel, 105-sodium hypochlorite barrel, 106-connecting pipe, 107-electromagnetic valve, 108-connecting plate, 109-water pump, 110-liquid outlet pipe, 111-fixing box, 112-sliding rail, 113-first sliding block, 114-first motor, 115-oil seal, 116-rotating disc, 117-sealing ring, 118-guide wheel, 119-supporting plate, 120-cam, 121-second motor, 122-connecting cylinder, 123-spring, 124-limiting block, 125-end cover, 126-connecting shaft, 127-disinfection box, 2-cutting device, 201-belt conveyer, 202-first chloric acid brush, 203-second chloric acid brush, 204-a second electric telescopic rod, 205-a connecting frame, 206-sterilized cotton, 207-a third motor, 208-a cutter, 209-an infrared induction lamp, 210-a third electric telescopic rod, 211-a pressing block, 212-a seventh motor, 213-a revolving drum, 214-an eighth motor, 215-a lead screw, 216-a moving block, 217-a side plate, 218-a placing frame, 219-a clamping plate, 220-a fifth electric telescopic rod, 3-a transplanting device, 301-a supporting column, 302-a rotating disc, 303-a ninth motor, 304-a press switch, 305-a disc, 306-a lug, 307-an inoculation bottle, 4-a bracket, 5-a controller, 6-a fourth motor, 7-a second sliding block, 8-a fourth electric telescopic rod, 9-a fifth motor, 10-a connecting seat, 11-a sixth motor, 12-a first adjusting screw, 13-a third sliding block, 14-a vertical rod, 15-a clamp, 16-an ultraviolet disinfection lamp and 17-a second adjusting screw.
Detailed Description
Referring to fig. 1-8, the present invention provides a technical solution:
a high-automatic plant tissue culture explant disinfection induction method and equipment comprise a support 4, wherein the left end of the support 4 is fixedly connected with a controller 5, the inner side of the bottom end of the support 4 is fixedly connected with a disinfection device 1, the left side of the disinfection device 1 is provided with a cutting device 2, the lower side of the cutting device 2 is provided with a transplanting device 3, the transplanting device 3 is fixedly connected with the support 4, the left end of the support 4 is fixedly connected with a fourth motor 11, the tail end of a main shaft of the fourth motor 11 is fixedly connected with a second adjusting screw 17, the second adjusting screw 17 is rotatably connected with the support 4, the outer side of the second adjusting screw 17 is spirally connected with a second sliding block 7, the second sliding block 7 is slidably connected with the support 4, the bottom end of the second sliding block 7 is fixedly connected with a fourth electric telescopic rod 8, the bottom end of the fourth electric telescopic rod 8 is fixedly connected with a fifth motor 9, and the tail end of the main shaft of the fifth motor 9 is fixedly connected with a connecting seat 10, the inboard fixedly connected with sixth motor 11 of right-hand member of connecting seat 10, the terminal fixedly connected with first adjusting screw 12 of main shaft of sixth motor 11, and first adjusting screw 12 rotates with connecting seat 10 to be connected, the outside screwed connection of first adjusting screw 12 has third slider 13, the bottom fixedly connected with vertical pole 14 of third slider 13, the bottom fixedly connected with anchor clamps 15 of vertical pole 14, the equal fixedly connected with ultraviolet ray disinfection lamp 16 in both sides of anchor clamps 15.
The disinfection device 1 comprises a water storage tank 101, a cooling pipe 102, a storage rack 103, an alcohol barrel 104, a sodium hypochlorite barrel 105, a connecting pipe 106, an electromagnetic valve 107, a connecting plate 108, a water pump 109, a liquid outlet pipe 110, a fixed box 111, a slide rail 112, a first slide block 113, a first motor 114, an oil seal 115, a rotary disc 116, a sealing ring 117, a guide wheel 118, a supporting plate 119, a cam 120, a second motor 121, a connecting cylinder 122, a spring 123, a limiting block 124, an end cover 125, a connecting shaft 126 and a disinfection box 127, wherein one end of a support 4 is fixedly connected with the water storage tank 101, the inner side of the water storage tank 101 is fixedly connected with the cooling pipe 102, the top end of the water storage tank 101 is fixedly connected with the storage rack 103, the inner side of the storage rack 103 is provided with the alcohol barrel 104 and the sodium hypochlorite barrel 105, the cooling pipe 102, the alcohol barrel 104 and the sodium hypochlorite barrel 105 are all communicated with the connecting pipe 106 through pipelines, the outlets of the cooling pipe 102, the alcohol barrel 104 and the sodium hypochlorite barrel 105 are all provided with the electromagnetic valve 107, a connecting plate 108 is fixedly connected to the right end of the water storage tank 101, a water pump 109 is fixedly connected to the top end of the connecting plate 108, the outlet end of the water pump 109 is communicated with a liquid outlet pipe 110, a disinfection box 127 is arranged at the outlet end of the liquid outlet pipe 110, a fixing box 111 is fixedly connected to one end of the support 4, a slide rail 112 is fixedly connected to the inner side of the top end of the fixing box 111, a first slide block 113 is slidably connected to the outer side of the slide rail 112, the disinfection box 127 is fixedly connected to the top end of the first slide block 113, a support plate 119 is fixedly connected to the inner side of the right end of the fixing box 111, a second motor 121 is fixedly connected to the rear end of the support plate 119, a cam 120 is fixedly connected to the end of a main shaft of the second motor 121, a guide wheel 118 is arranged on one side of the cam 120, the guide wheel 118 is fixedly connected to the disinfection box 127, a connecting cylinder 122 is fixedly connected to the inner side of the left end of the fixing box 111, a spring 123 is fixedly connected to the spring 123, and a limit block 124 is fixedly connected to the other end of the spring 123, the cutting device 2 comprises a belt conveyor 201, a first chloric acid brush 202, a second chloric acid brush 203, a second electric telescopic rod 204, a connecting frame 205, sterilized cotton 206, a third motor 207, a cutter 208, an infrared induction lamp 209, a connecting shaft 126, a sterilizing box 127, an end cover 125, a sealing ring 117 and an oil seal 115, wherein the outer side of a transmission shaft of the first motor 114 is rotatably connected with the outer side of the connecting shaft 126, the connecting shaft 126 is fixedly connected with the right end of the limiting block 124, the connecting shaft 126 is fixedly connected with the sterilizing box 127, the outer side of the connecting shaft 126 is slidably connected with the end cover 125, the end cover 125 is fixedly connected with the connecting cylinder 122, the bottom end of the sterilizing box 127 is fixedly connected with the first motor 114, the tail end of the main shaft of the first motor 114 is fixedly connected with the rotary table 116, the rotary table 116 is rotatably connected with the sterilizing box 127, the oil seal 115 is fixedly connected with the sterilizing box 127, the cutting device 2 comprises a belt conveyor 201, a first chloric acid brush 202, a second chloric acid brush 203, a second electric telescopic rod 204, a connecting frame 205, sterilized cotton 206, a third motor 207, a cutter 208, an infrared induction lamp 209, a sterilizing lamp, The device comprises a third electric telescopic rod 210, a pressing block 211, a seventh motor 212, a rotary drum 213, an eighth motor 214, a lead screw 215, a moving block 216, a side plate 217, a placing frame 218, a clamping plate 219 and a fourth electric telescopic rod 220, wherein one end of the support 4 is fixedly connected with a belt conveyor 201, one end of the support 4 is fixedly connected with the seventh motor 212, the tail end of a main shaft of the seventh motor 212 is fixedly connected with the rotary drum 213, the inner side of the rotary drum 213 is fixedly connected with the eighth motor 214, the tail end of the main shaft of the eighth motor 214 is fixedly connected with the lead screw 215, the outer side of the lead screw 215 is spirally connected with the moving block 216, one end of the moving block 216 is fixedly connected with the side plate 217, one end of the side plate 217 is fixedly connected with the placing frame 218, the inner side of the placing frame 218 is fixedly connected with the fifth electric telescopic rod 220, and the other end of the fifth electric telescopic rod 220 is fixedly connected with the clamping plate 219, one end of the support 4 is fixedly connected with a third electric telescopic rod 210, the other end of the third electric telescopic rod 210 is fixedly connected with a pressing block 211, the bottom end of the belt conveyor 201 is provided with a first chloric acid brush 202, the first chloric acid brush 202 is fixedly connected with the support 4, the belt conveyor 201 can be disinfected through the first chloric acid brush 202, the bottom end of the rotary drum 213 is provided with a second chloric acid brush 203, the second chloric acid brush 203 is fixedly connected with the support 4, the disinfection of a clamping plate 219 on the inner side of the rotary drum 203 can be realized through the second chloric acid brush 203, one end of the support 4 is fixedly connected with a second electric telescopic rod 204, the other end of the second electric telescopic rod 204 is fixedly connected with a connecting frame 205, one end of the connecting frame 205 is fixedly connected with a third motor 207, and the tail end of a main shaft of the third motor 207 is fixedly connected with a cutter 208, the both sides of cutter 208 are provided with aseptic cotton 206, and aseptic cotton 206 and connection frame 205 fixed connection, the bottom fixedly connected with infrared induction lamp 209 of connection frame 205, transplanting device 3 includes support column 301, carousel 302, ninth motor 303, press switch 304, disc 305, lug 306 and inoculation bottle 307, the inboard fixedly connected with ninth motor 303 in top of support column 301, the terminal fixedly connected with carousel 302 of main shaft of ninth motor 303 upside, the terminal fixedly connected with disc 305 of main shaft of ninth motor 303 downside, the outside fixedly connected with lug 306 of disc 305, the recess that is used for inserting the culture dish is seted up to the inboard of carousel 302, the recess quantity on the carousel 302 is the same with the lug quantity of 306 on the disc 305.
The method comprises the following steps:
the method comprises the following steps: firstly, disinfecting the plant tissue culture through a disinfection device (1);
step two: the disinfected plant tissue culture is cut in sections through a cutting device (2);
step three: and (4) inoculating the plant tissue culture after the segmentation and cutting through a transplanting device (3).
The working process is as follows: before the invention is used, power is supplied by an external power supply, a cooling pipe 102 is communicated with an external autoclave, the inner side of the right end of a sterilizing box 127 is externally connected with a drain pipe, the outer side of the drain pipe is communicated with a water pump, a worker firstly places plant tissue culture seedlings on the inner side of the sterilizing box 127, then controls an electromagnetic valve communicated with an alcohol barrel 104 to be opened by a controller 5, closes the rest electromagnetic valves, sprays 75% alcohol on the inner side of the alcohol barrel 104 into the inner side of the sterilizing box 127 through a connecting pipe 106 and a liquid outlet pipe 110 by a water pump 109, soaks for 30S, meanwhile, a second motor 121 pushes the sterilizing box 127 through a cam 120 and a guide wheel 118, and the sterilizing box 127 can shake back and forth under the action of a spring 123, so that the 75% alcohol on the inner side of the sterilizing box 127 can be sterilized while shaking, and the 75% alcohol on the inner side of the sterilizing box 127 is discharged out of the sterilizing box through the external drain pipe and the water pump 127 after the sterilization is finished, then closing an electromagnetic valve 107 communicated with an alcohol barrel 104, opening the electromagnetic valve 107 communicated with a cooling pipe 102, enabling the sterile water inside the autoclave to pass through the cooling pipe 102, cooling the sterile water through the cooling pipe 102, enabling the sterile water to flow to the inner side of a sterilizing box 127 under the action of a water pump 109, enabling the sterile water to sink plant tissue culture seedlings, enabling a first motor 114 to drive a rotary disc 116 to rotate clockwise on the inner side of the sterilizing box 127, enabling the sterile water inside the sterilizing box 127 to rotate clockwise under the action of the rotary disc 116, discharging the sterile water out of the sterilizing box 127 through an external drain pipe and a water suction pump after the washing is finished, then performing secondary sterilization on the plant tissue culture seedlings by using 2% sodium hypochlorite solution according to the above mode, performing sterilization while shaking, flushing three times by using sterile water after the sterilization is finished, and then discharging the sterile water from the inner side of the sterilizing box 127, then, a plant tissue culture seedling is independently placed into the cutting cylinder 202 for cutting through the clamp 15, a worker controls the fourth motor 6 to drive the second adjusting screw 17 to spirally rotate on the inner side of the second slide block 7 through the control cabinet 5, so that the second slide block 7 drives the clamp 15 and the ultraviolet disinfection lamp 16 to be positioned on the plant tissue culture seedling through the fourth electric telescopic rod 8, the connecting seat 10, the first adjusting screw 12 and the vertical rod 14, then the plant tissue culture seedling is clamped through the clamp 15, meanwhile, the ultraviolet disinfection lamp 16 can disinfect the plant tissue culture seedling, the plant tissue culture is placed on the belt conveyor transfer 201 through the clamp 15, the plant tissue culture is conveyed to the lower side of the cutter 208 through the belt conveyor 201, meanwhile, the infrared induction lamp 209 senses a node of the plant tissue culture, and then the third electric telescopic rod 210 is controlled by the controller 5 to drive the pressing block 211 to press the plant tissue culture, ensuring that the plant tissue culture does not deviate in the cutting process, simultaneously driving the cutter 208 to rotate by the third motor 207, driving the cutter 208 to rotate by the second electric telescopic rod 204 through the connecting frame 205 and the third motor 207, driving the cutter 208 to cut the plant tissue culture after the cutting is finished, continuously rotating the cutter 208 by the cutting cotton 206, sterilizing the cutter 208 by the cut plant tissue culture, dropping the cut plant tissue culture between the clamping plates 219, driving the clamping plates 219 by the fifth electric telescopic rod 220 to clamp the plant tissue culture, driving the tap 215 to spirally rotate at the inner side of the moving block 216 by the eighth motor 214, driving the moving block 216 to work in the direction of the inoculation bottle 307 by the side plates 217, the placing frame 218, the fifth electric telescopic rod 220 and the clamping plates 219, enabling the transplanting device 3 to work at the moment, inserting the turntable 302 on the transplanting device 3 into a culture dish, driving the ninth motor 303 to work, driving the turntable 302 and the disc 305 to synchronously rotate by the ninth motor 303, after the bump 306 on the disc 305 presses the press switch 304, the fourth motor 303 stops rotating, at this time, the culture dish on the turntable 302 is just opposite to the right end of the plant rhizome, and the plant rhizome can continue to move rightwards to enter the inner side of the culture dish, so that the transplantation of the plant rhizome is realized.
The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts of the present invention. The foregoing is only a preferred embodiment of the present invention, and it should be noted that there are objectively infinite specific structures due to the limited character expressions, and it will be apparent to those skilled in the art that a plurality of modifications, decorations or changes may be made without departing from the principle of the present invention, and the technical features described above may be combined in a suitable manner; such modifications, variations, combinations, or adaptations of the invention using its spirit and scope, as defined by the claims, may be directed to other uses and embodiments.
Claims (10)
1. A high automatic plant tissue culture explant disinfection induction apparatus, includes support (4), its characterized in that: the left end of the support (4) is fixedly connected with a controller (5), the bottom inner side of the support (4) is fixedly connected with a disinfection device (1), the left side of the disinfection device (1) is provided with a cutting device (2), the lower side of the cutting device (2) is provided with a transplanting device (3), the transplanting device (3) is fixedly connected with the support (4), the left end of the support (4) is fixedly connected with a fourth motor (11), the tail end of a main shaft of the fourth motor (11) is fixedly connected with a second adjusting screw (17), the second adjusting screw (17) is rotatably connected with the support (4), the outer side of the second adjusting screw (17) is spirally connected with a second sliding block (7), the second sliding block (7) is slidably connected with the support (4), the bottom end of the second sliding block (7) is fixedly connected with a fourth electric telescopic rod (8), the utility model discloses a portable electric sterilizing lamp, including the bottom fixedly connected with fifth motor (9) of fourth electric telescopic handle (8), the terminal fixedly connected with connecting seat (10) of main shaft of fifth motor (9), the inboard fixedly connected with sixth motor (11) of right-hand member of connecting seat (10), the terminal fixedly connected with first adjusting screw (12) of main shaft of sixth motor (11), and first adjusting screw (12) rotate with connecting seat (10) and be connected, the outside screwed connection of first adjusting screw (12) has third slider (13), the bottom fixedly connected with vertical pole (14) of third slider (13), the bottom fixedly connected with anchor clamps (15) of vertical pole (14), the equal fixedly connected with ultraviolet ray disinfection lamp (16) in both sides of anchor clamps (15).
2. The highly automated plant tissue culture explant sterilization induction apparatus of claim 1, wherein: the disinfection device (1) comprises a water storage tank (101), a cooling pipe (102), a storage rack (103), an alcohol barrel (104), a sodium hypochlorite barrel (105), a connecting pipe (106), an electromagnetic valve (107), a connecting plate (108), a water pump (109), a liquid outlet pipe (110), a fixed box (111), a sliding rail (112), a first sliding block (113), a first motor (114), an oil seal (115), a rotating disc (116), a sealing ring (117), a guide wheel (118), a supporting plate (119), a cam (120), a second motor (121), a connecting cylinder (122), a spring (123), a limiting block (124), an end cover (125), a connecting shaft (126) and a disinfection box (127), wherein one end of the support (4) is fixedly connected with the water storage tank (101), the cooling pipe (102) is fixedly connected to the inner side of the water storage tank (101), the storage rack (103) is fixedly connected to the top end of the water storage tank (101), the inboard of storage rack (103) is provided with alcohol bucket (104) and sodium hypochlorite bucket (105), cooling tube (102), alcohol bucket (104) and sodium hypochlorite bucket (105) all communicate through pipeline and connecting pipe (106), the exit of cooling tube (102), alcohol bucket (104) and sodium hypochlorite bucket (105) all is provided with solenoid valve (107), the right-hand member fixedly connected with connecting plate (108) of storage water tank (101), the top fixedly connected with water pump (109) of connecting plate (108), the exit end intercommunication of water pump (109) has drain pipe (110), the exit end of drain pipe (110) is provided with disinfect box (127).
3. The highly automated plant tissue culture explant sterilization induction apparatus of claim 1, wherein: one end of the support (4) is fixedly connected with a fixed box (111), the inner side of the top end of the fixed box (111) is fixedly connected with a slide rail (112), the outer side of the slide rail (112) is connected with a first slide block (113) in a sliding manner, the top end of the first slide block (113) is fixedly connected with a disinfection box (127), the inner side of the right end of the fixed box (111) is fixedly connected with a support plate (119), the rear end of the support plate (119) is fixedly connected with a second motor (121), the tail end of a main shaft of the second motor (121) is fixedly connected with a cam (120), one side of the cam (120) is provided with a guide wheel (118), the guide wheel (118) is fixedly connected with the disinfection box (127), the inner side of the left end of the fixed box (111) is fixedly connected with a connecting cylinder (122), the inner side of the connecting cylinder (122) is fixedly connected with a spring (123), and the other end of the spring (123) is fixedly connected with a limit block (124), and stopper (124) and connecting cylinder (122) sliding connection, the right-hand member fixedly connected with connecting axle (126) of stopper (124), and connecting axle (126) and disinfect box (127) fixed connection, the outside sliding connection of connecting axle (126) has end cover (125), and end cover (125) and connecting cylinder (122) fixed connection.
4. The highly automated plant tissue culture explant sterilization induction apparatus of claim 2, wherein: the bottom end fixedly connected with first motor (114) of disinfect box (127), the terminal fixedly connected with carousel (116) of main shaft of first motor (114), and carousel (116) rotate with disinfect box (127) and be connected, the outside fixedly connected with sealing ring (117) of carousel (116), and sealing ring (117) rotate with disinfect box (127) and be connected, the transmission shaft outside of first motor (114) rotates and is connected with oil blanket (115), and oil blanket (115) and disinfect box (127) fixed connection.
5. The highly automated plant tissue culture explant sterilization induction apparatus of claim 1, wherein: the cutting device (2) comprises a belt conveyor (201), a first chloric brush (202), a second chloric brush (203), a second electric telescopic rod (204), a connecting frame (205), sterilized cotton (206), a third motor (207), a cutter (208), an infrared induction lamp (209), a third electric telescopic rod (210), a pressing block (211), a seventh motor (212), a rotary drum (213), an eighth motor (214), a screw rod (215), a moving block (216), a side plate (217), a placing frame (218), a clamping plate (219) and a fourth electric telescopic rod (220), wherein the belt conveyor (201) is fixedly connected with one end of the support (4), the seventh motor (212) is fixedly connected with one end of the support (4), the rotary drum (213) is fixedly connected with the tail end of a main shaft of the seventh motor (212), and an eighth motor (214) is fixedly connected with the inner side of the rotary drum (213), the tail end of a main shaft of the eighth motor (214) is fixedly connected with a lead screw (215), the outer side of the lead screw (215) is spirally connected with a moving block (216), one end of the moving block (216) is fixedly connected with a side plate (217), one end of the side plate (217) is fixedly connected with a placing frame (218), the inner side of the placing frame (218) is fixedly connected with a fifth electric telescopic rod (220), and the other end of the fifth electric telescopic rod (220) is fixedly connected with a clamping plate (219).
6. The highly automated plant tissue culture explant sterilization induction apparatus of claim 5, wherein: one end fixedly connected with third electric telescopic handle (210) of support (4), the other end fixedly connected with briquetting (211) of third electric telescopic handle (210).
7. The highly automated plant tissue culture explant sterilization induction apparatus of claim 6, wherein: the bottom of belt conveyor (201) is provided with chloric acid brush (202) for the first time, and chloric acid brush (202) for the first time and support (4) fixed connection, the bottom of rotary drum (213) is provided with chloric acid brush (203) for the second time, and chloric acid brush (203) for the second time and support (4) fixed connection.
8. The highly automated plant tissue culture explant sterilization induction apparatus of claim 5, wherein: the one end fixedly connected with second electric telescopic handle (204) of support (4), the other end fixedly connected with link (205) of second electric telescopic handle (204), the one end fixedly connected with third motor (207) of link (205), the terminal fixedly connected with cutter (208) of main shaft of third motor (207), the both sides of cutter (208) are provided with aseptic cotton (206), and aseptic cotton (206) and link (205) fixed connection, the bottom fixedly connected with infrared induction lamp (209) of link (205).
9. The highly automated plant tissue culture explant sterilization induction apparatus of claim 1, wherein: transplanting device (3) are including support column (301), carousel (302), ninth motor (303), press switch (304), disc (305), lug (306) and inoculation bottle (307), the inboard fixedly connected with ninth motor (303) in top of support column (301), the terminal fixedly connected with carousel of main shaft (302) upside of ninth motor (303), the terminal fixedly connected with disc of main shaft (305) of ninth motor (303) downside, the outside fixedly connected with lug (306) of disc (305), the recess that is used for inserting the culture dish is seted up to the inboard of carousel (302), recess quantity on carousel (302) is the same with lug (306) quantity on disc (305).
10. The highly automated plant tissue culture explant sterilization induction method of claim 1, wherein:
the method comprises the following steps:
the method comprises the following steps: firstly, disinfecting the plant tissue culture through a disinfection device (1);
step two: the disinfected plant tissue culture is cut in sections through a cutting device (2);
step three: and (4) inoculating the plant tissue culture after the segmentation and cutting through a transplanting device (3).
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Cited By (1)
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| CN115281088A (en) * | 2022-07-28 | 2022-11-04 | 华南农业大学 | Plant tissue culture system |
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