CN112841039A - Integrated plant tissue culture device - Google Patents
Integrated plant tissue culture device Download PDFInfo
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- CN112841039A CN112841039A CN202110394127.6A CN202110394127A CN112841039A CN 112841039 A CN112841039 A CN 112841039A CN 202110394127 A CN202110394127 A CN 202110394127A CN 112841039 A CN112841039 A CN 112841039A
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- tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses an integrated plant tissue culture device in the technical field of plant tissue culture devices, which comprises a tissue culture bottle and a washing bottle, wherein one side of the tissue culture bottle is transversely connected with a convex pipe, the middle of the bottom of the convex pipe is vertically connected with a cleaning pipe, the top end of the washing bottle is connected with a hose, and the other end of the hose is connected with the top wall of the convex pipe in a penetrating way; the invention can make the plant breed and produce non-toxic plant without the limitation of growing season, and shorten the culture period of the plant; the integrated plant tissue culture device can simply perform tissue culture tests in a bacteria-containing environment, and avoids complex and complicated operation steps; the problem that bacteria are easily brought to a culture medium carelessly in the inoculation process to cause the growth of the bacteria is solved; the environment is cultivated to intelligent control group, reduces artifical contact, keeps the environment clean.
Description
Technical Field
The invention relates to the technical field of plant tissue cultivators, in particular to an integrated plant tissue culture device.
Background
Plant tissue culture, namely a plant sterile culture technology, also called in vitro culture, is a disciplinary which utilizes isolated organs (such as roots, stems, leaves, stem tips, flowers, fruits and the like), tissues (such as cambium, epidermis, cortex, medulla cells, endosperm and the like) or cells (such as megaspore, microspore, somatic cells and the like) and protoplast of a plant body to induce callus, adventitious buds and adventitious roots under the artificial conditions of sterility, proper artificial culture medium, temperature and the like according to the theory that plant cells have totipotency. The plant tissue culture technology occupies small space and is not limited by regions and seasons. Although the virus diseases of crops are a serious problem in current agricultural production, a large number of virus-free seedlings can be obtained by using cells in a meristematic region for tissue culture. The culture period of the tissue culture plant is short, mass propagation can be carried out in a short time, the tissue culture plant is used for saving endangered plants, the problem that some plants have few or no seeds is solved, no variation exists, and all genetic characteristics of the original parent can be maintained. The tissue culture process is convenient to manage, the industrial production and the automatic control are utilized, the investment is low, and the economic benefit is high.
Plant tissue culture has high requirements on sterile environment, the complicated culture steps require preparation of a large amount of equipment and medicines, and finally, the operation flow is complex, special training is not available, and an experienced incubator is difficult to succeed. That is, in order to culture plant tissues, a large amount of equipment and space are required, and therefore, the operation is only suitable for batch production in a large laboratory.
Based on the design, the invention designs the integrated plant tissue culture device which adopts an integrated full-sealed design, then the prepared culture medium is loaded into the culture device, the culture device is sealed, and the sterilization and disinfection are carried out fully to provide a sterile environment, the culture device can simply carry out tissue culture test in the sterile environment, and complicated operation steps are avoided.
Disclosure of Invention
The present invention is directed to an integrated plant tissue culture device to solve the above-mentioned problems of the background art.
In order to achieve the purpose, the invention provides the following technical scheme: the integrated plant tissue culture device comprises a tissue culture bottle and a washing bottle, wherein one side of the tissue culture bottle is transversely connected with a convex tube, the middle of the bottom of the convex tube is vertically connected with a cleaning tube, the top end of the washing bottle is connected with a hose, and the other end of the hose penetrates through the top wall of the convex tube;
the top of the tissue culture bottle is connected with a breathable cover, one side, far away from the convex tube, of the tissue culture bottle is provided with a side wall opening, the outer side of the side wall opening is hermetically connected with a first plastic film, tweezers are arranged on the inner side of the tissue culture bottle, the tweezers handle ends of the tweezers are connected with the first plastic film in a penetrating mode and extend out, and the penetrating connection position of the tweezers and the first plastic film is subjected to sealing treatment;
the left end of the convex tube is provided with an opening, the outer side of the opening is connected with a second plastic film in a sealing mode, the inner side of the convex tube is provided with a material pushing rod, the front side of the top of the convex tube is provided with a feeding hole, a silica gel plug is arranged on the feeding hole, the left side of the top surface of the convex tube is vertically provided with an injector, and the bottom of the injector penetrates through the top wall of the convex tube in a sealing mode;
the center of scavenge pipe is vertical to be provided with the lifting rod, the roof of protruding pipe is run through in the top activity of lifting rod, the bottom of lifting rod is provided with the screen cloth, the through connection department of lifting rod and protruding pipe is through sealed processing, the bottom middle part of scavenge pipe is provided with the drain pipe, be provided with the check valve on the drain pipe.
Preferably, sterile water is filled in the washing bottle, and the washing bottle is a plastic soft bottle.
Preferably, the injector adopts a medical injection needle cylinder, disinfectant is filled in the injector, a guide pipe is arranged at the bottom of a water outlet of the injector, and the bottom end of the guide pipe faces the center of the cleaning pipe.
Preferably, the plastic sleeve is connected to the outer side of the penetrating connection position of the lifting rod and the convex pipe in a sealing mode, the lifting rod is located in the plastic sleeve, and the top of the plastic sleeve is bonded to the upper portion of the lifting rod in a sealing mode.
Preferably, one end of the material pushing rod, which is positioned at the inner side of the convex pipe, is fixedly connected with a material shovel.
Preferably, a water stop clamp is arranged on the drain pipe.
Preferably, group banks up the bottle with earth and still be equipped with temperature sensor, photometer, temperature regulator and LED vegetation lamp, the equal electric connection singlechip of temperature sensor, photometer, temperature regulator and LED vegetation lamp, group banks up the bottle outside with earth and still be connected with CO2 gas cylinder through the trachea, CO2 gas cylinder gas outlet end is equipped with the electromagnetism pneumatic valve, electromagnetism pneumatic valve electric connection singlechip.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention can make the plant breed and produce non-toxic plant without the limitation of growing season, and shorten the culture period of the plant;
2. the integrated plant tissue culture device can simply perform tissue culture tests in a bacteria-containing environment, and avoids complex and complicated operation steps;
3. the problem that bacteria are easily brought to a culture medium carelessly in the inoculation process to cause the growth of the bacteria is solved; 4. the environment is cultivated to intelligent control group, reduces artifical contact, keeps the environment clean.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a three-dimensional schematic view of a first perspective of the present invention;
FIG. 2 is a second perspective three-dimensional view of the present invention;
FIG. 3 is a three-dimensional schematic view of a third perspective of the present invention;
FIG. 4 is a cross-sectional view of a first perspective of the present invention;
FIG. 5 is a cross-sectional structural view from a second perspective of the present invention;
FIG. 6 is a schematic cross-sectional view of a portion of the present invention;
FIG. 7 is an electrical block diagram of the present invention;
FIG. 8 is a flow chart of the method of the present invention.
1-tissue culture bottle, 2-washing bottle, 3-convex tube, 4-washing tube, 5-soft tube, 6-ventilating cover, 7-first plastic film, 8-tweezers, 9-second plastic film, 10-material pushing rod, 11-silica gel plug, 12-water discharging tube, 13-pulling rod, 14-screen, 15-injector and 16-one-way valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 8, the present invention provides a technical solution: the integrated plant tissue culture device comprises a tissue culture bottle 1 and a washing bottle 2, wherein one side of the tissue culture bottle 1 is transversely connected with a convex tube 3, the middle of the bottom of the convex tube 3 is vertically connected with a cleaning tube 4, the top end of the washing bottle 2 is connected with a hose 5, and the other end of the hose 5 is connected with the top wall of the convex tube 3 in a penetrating manner;
the top of the tissue culture bottle 1 is connected with a breathable cover 6, one side, away from the convex tube 3, of the tissue culture bottle 1 is provided with a side wall opening, the outer side of the side wall opening is hermetically connected with a first plastic film 7, the inner side of the tissue culture bottle 1 is provided with a pair of tweezers 8, the tweezers handle ends of the tweezers 8 are connected with the first plastic film 7 in a penetrating mode and extend outwards, and the penetrating connection position of the tweezers 8 and the first plastic film 7 is subjected to sealing treatment;
the left end of the convex tube 3 is provided with an opening, the outer side of the opening is connected with a second plastic film 9 in a sealing mode, the inner side of the convex tube 3 is provided with a material pushing rod 10, the front side of the top of the convex tube 3 is provided with a feeding hole, a silica gel plug 11 is arranged on the feeding hole, the left side of the top surface of the convex tube 3 is vertically provided with an injector 15, and the bottom of the injector 15 penetrates through the top wall of the convex tube 3 in;
the vertical lifting rod 13 that is provided with in the center of scavenge pipe 4, the roof of protruding pipe 3 is run through in the top activity of lifting rod 13, and the bottom of lifting rod 13 is provided with screen cloth 14, and the through connection department of lifting rod 13 and protruding pipe 3 is through sealed processing, and the bottom middle part of scavenge pipe 4 is provided with drain pipe 12, is provided with check valve 16 on the drain pipe 12.
Furthermore, sterile water is filled in the washing bottle 2, and the washing bottle 2 is a plastic soft bottle.
Further, the injector 15 adopts a medical injection needle cylinder, disinfectant is filled in the injector 15, a guide pipe is arranged at the bottom of a water outlet of the injector 15, and the bottom end of the guide pipe faces the center of the cleaning pipe 4.
Furthermore, the outer side of the penetrating connection part of the lifting rod 13 and the convex pipe 3 is hermetically connected with a plastic sleeve, the lifting rod 13 is positioned in the plastic sleeve, and the top of the plastic sleeve is hermetically bonded with the upper part of the lifting rod 13.
Furthermore, a material shovel is fixedly connected to one end of the material pushing rod 10, which is located inside the convex pipe 3.
Furthermore, a water stop clip is provided on the drain pipe 12.
Further, the tissue culture bottle 1 is also provided with a temperature sensor, a photometer, a temperature regulator and an LED plant growth lamp, wherein the temperature sensor, the photometer, the temperature regulator and the LED plant growth lamp are all electrically connected with the single chip microcomputer, the outer side of the tissue culture bottle is also connected with a CO2 gas cylinder through a gas pipe, an electromagnetic gas valve is arranged at the gas outlet end of the CO2 gas cylinder, and the electromagnetic gas valve is electrically connected with the single chip microcomputer; according to the measured environmental value, the temperature, the humidity and the illumination in the tissue culture bottle are automatically controlled according to the temperature, the humidity and the light requirement data measured by various plants for tissue culture. In addition, the tissue culture bottle also carries a CO2 gas bottle, and the tissue culture bottle is ventilated regularly to supplement a carbon source for the growth of tissue culture seedlings.
The device comprises the following steps:
1. primary disinfection of explant outside culture device
In use, the plants are first sampled and the explants are washed clean with tap water and disinfected with 75% alcohol for 8-30 seconds, then rinsed 2 times with tap water, then the material is immersed in a 0.1% solution of HgCl2, shaken gently, disinfected for 5-12 minutes, and then rinsed 4-5 times with tap water. Finally soaking the mixture in 100ml of disinfectant for 30-60 minutes;
2. injecting the explant into a culture device, but avoiding contact with the culture medium
Then, opening a silica gel plug on the left side of the integrated plant tissue culture device, and placing the plant sample on a stainless steel mesh screen on the left side through a feed port of the silica gel plug by using forceps to prevent the explant subjected to preliminary disinfection from directly contacting a culture medium;
3. secondary disinfection of explants in the left closed space
The key step is to lightly push the pressing rod of the upper needle cylinder, inject the disinfectant into the mesh screen, disinfect the sample, open the water stop valve after disinfection, and discharge the used disinfectant through the one-way valve. Then pinching the bottle body with the left side filled with sterile water, injecting the sterile water onto the mesh screen, repeatedly washing the sample tissue, and discharging waste liquid through a water outlet at the lower part; repeating the steps for multiple times, and fully performing secondary disinfection on the explants;
4. transferring the explant to a right tissue culture bottle by using a feeding rod for inoculation
The explant after the secondary disinfection is pushed into a tissue culture bottle by pushing the feeding rod, and then the right tweezers are operated to fully insert the explant into a culture medium to be contacted with the culture medium. Finally, the culture is completed.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above disclosure of further embodiments of the invention is intended only to facilitate the explanation of the invention. Further examples are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (7)
1. The integrated plant tissue culture device is characterized by comprising a tissue culture bottle (1) and a washing bottle (2), wherein one side of the tissue culture bottle (1) is transversely connected with a convex tube (3), the middle of the bottom of the convex tube (3) is vertically connected with a cleaning tube (4), the top end of the washing bottle (2) is connected with a hose (5), and the other end of the hose (5) is connected with the top wall of the convex tube (3) in a penetrating manner;
the top of the tissue culture bottle (1) is connected with a breathable cover (6), one side, away from the convex tube (3), of the tissue culture bottle (1) is provided with a side wall opening, the outer side of the side wall opening is connected with a first plastic film (7) in a sealing mode, tweezers (8) are arranged on the inner side of the tissue culture bottle (1), the tweezers handle ends of the tweezers (8) are connected with the first plastic film (7) in a penetrating mode and extend outwards, and the penetrating connection position of the tweezers (8) and the first plastic film (7) is subjected to sealing treatment;
the left end of the convex tube (3) is provided with an opening, the outer side of the opening is connected with a second plastic film (9) in a sealing mode, a material pushing rod (10) is arranged on the inner side of the convex tube (3), a feeding hole is formed in the front side of the top of the convex tube (3), a silica gel plug (11) is arranged on the feeding hole, an injector (15) is vertically arranged on the left side of the top surface of the convex tube (3), and the bottom of the injector (15) penetrates through the top wall of the convex tube (3) in a sealing mode;
the center of scavenge pipe (4) is vertical to be provided with and to carry pull rod (13), the roof of protruding pipe (3) is run through in the top activity of carrying pull rod (13), the bottom of carrying pull rod (13) is provided with screen cloth (14), the through connection department of carrying pull rod (13) and protruding pipe (3) is through sealed processing, the bottom middle part of scavenge pipe (4) is provided with drain pipe (12), be provided with check valve (16) on drain pipe (12).
2. The integrated plant tissue culture incubator of claim 1, wherein: sterile water is filled in the washing bottle (2), and the washing bottle (2) is a plastic soft bottle.
3. The integrated plant tissue culture incubator of claim 1, wherein: the medical syringe is adopted as the injector (15), disinfectant is filled in the injector (15), a guide pipe is arranged at the bottom of a water outlet of the injector (15), and the bottom end of the guide pipe faces the center of the cleaning pipe (4).
4. The integrated plant tissue culture incubator of claim 1, wherein: the outer side of the penetrating connection part of the lifting rod (13) and the convex pipe (3) is hermetically connected with a plastic sleeve, the lifting rod (13) is positioned in the plastic sleeve, and the top of the plastic sleeve is hermetically bonded with the upper part of the lifting rod (13).
5. The integrated plant tissue culture incubator of claim 1, wherein: and one end of the pushing rod (10) positioned at the inner side of the convex pipe (3) is fixedly connected with a material shovel.
6. The integrated plant tissue culture incubator of claim 1, wherein: and a water stop clamp is arranged on the drain pipe (12).
7. The integrated plant tissue culture incubator of claim 1, wherein: group banks up bottle (1) with earth and still be equipped with temperature sensor, photometer, temperature regulator and LED vegetation lamp, the equal electric connection singlechip of temperature sensor, photometer, temperature regulator and LED vegetation lamp, group banks up the bottle outside with earth and still be connected with CO2 gas cylinder through the trachea, CO2 gas cylinder gas outlet end is equipped with the electromagnetism pneumatic valve, electromagnetism pneumatic valve electric connection singlechip.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110394127.6A CN112841039A (en) | 2021-04-13 | 2021-04-13 | Integrated plant tissue culture device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110394127.6A CN112841039A (en) | 2021-04-13 | 2021-04-13 | Integrated plant tissue culture device |
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| CN112841039A true CN112841039A (en) | 2021-05-28 |
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| CN202110394127.6A Pending CN112841039A (en) | 2021-04-13 | 2021-04-13 | Integrated plant tissue culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113455390A (en) * | 2021-06-10 | 2021-10-01 | 江西环境工程职业学院 | High-automatic plant tissue culture explant disinfection induction method and equipment |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207443862U (en) * | 2017-09-04 | 2018-06-05 | 东北林业大学 | A kind of multi-functional tissue culture bottle |
| CN211241206U (en) * | 2019-09-05 | 2020-08-14 | 淮北师范大学 | Integration plant tissue culture device |
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2021
- 2021-04-13 CN CN202110394127.6A patent/CN112841039A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207443862U (en) * | 2017-09-04 | 2018-06-05 | 东北林业大学 | A kind of multi-functional tissue culture bottle |
| CN211241206U (en) * | 2019-09-05 | 2020-08-14 | 淮北师范大学 | Integration plant tissue culture device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113455390A (en) * | 2021-06-10 | 2021-10-01 | 江西环境工程职业学院 | High-automatic plant tissue culture explant disinfection induction method and equipment |
| CN113455390B (en) * | 2021-06-10 | 2024-06-11 | 江西环境工程职业学院 | High-automatic plant tissue culture explant disinfection induction method and equipment |
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Application publication date: 20210528 |