CN105706880A - Purple sweet potato virus-free seedling water culture fast breeding method - Google Patents

Purple sweet potato virus-free seedling water culture fast breeding method Download PDF

Info

Publication number
CN105706880A
CN105706880A CN201610144339.8A CN201610144339A CN105706880A CN 105706880 A CN105706880 A CN 105706880A CN 201610144339 A CN201610144339 A CN 201610144339A CN 105706880 A CN105706880 A CN 105706880A
Authority
CN
China
Prior art keywords
seedling
seedlings
root
sweet potato
nutritional solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610144339.8A
Other languages
Chinese (zh)
Inventor
朱金霞
张源沛
郑国保
孔德杰
李苗
曲继松
张丽娟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ningxia Academy of Agriculture and Forestry Sciences
Original Assignee
Ningxia Academy of Agriculture and Forestry Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ningxia Academy of Agriculture and Forestry Sciences filed Critical Ningxia Academy of Agriculture and Forestry Sciences
Priority to CN201610144339.8A priority Critical patent/CN105706880A/en
Publication of CN105706880A publication Critical patent/CN105706880A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Abstract

The invention provides a purple sweet potato virus-free seedling water culture fast breeding method, and belongs to the field of crop seedling culture. The method comprises the following steps of 1, performing sterilization: shearing sweet potato virus-free seedling stem tops with the length of 2.0cm to 3.0cm; fixing the sweet potato virus-free seedling stem tops into foam plate holes by sponge or cotton; floating the sweet potato virus-free seedling stem tops in a 0.1-percent carbendazim solution for soaking the roots for 30min; then, performing flushing for 3 to 5 times; 2, performing rooting: putting seedlings sterilized in the first step into a water culture seedling culture disc for culturing 5 to 7 days through the still standing floating in a rooting nutrition solution until the water roots grow to 1.0cm to 3.0cm; 3, performing culture: culturing the purple sweet potato seedlings with the water roots in the second step in a strong seedling nutrition liquid for 12 to 15d in a nutrition solution; using the seedlings as mother seedlings when 4 to 5 leaves grow; 4, shearing stem tops with the length being 2.0cm to 3.0cm from the mother seedlings cultured in the third step, and performing sterilization, rooting and culturing; 5, shearing the stem tips on the other seedlings obtained in the third step for 8 to 10 times and then using the mother seedlings as seed seedlings to be transplanted. When the method provided by the invention is used for breeding the purple sweet potato virus-free seedlings, the production cost is low; the quality is high.

Description

A kind of method that Rhizoma Steudnerae Henryanae detoxic seedling water planting is numerous soon
Technical field
The invention belongs to crops seedling-raising technique field, particularly the water planting quick-breeding method of a kind of Rhizoma Steudnerae Henryanae detoxic seedling.
Background technology
The modes of reproduction of current Rhizoma Steudnerae Henryanae detoxic seedling mainly has two kinds, and a kind of is that the branch that detoxification original silkworm egg potato grows carries out cottage propagation, and this method repoductive time is long, and survival rate is low, it is easy to bacteria infection, causes that the production cost of original silkworm egg is high;Another kind is to breed in Rhizoma Steudnerae Henryanae detoxification test tube plantlet culture medium based on MS, and this method reproduction speed ratio is very fast, and survival rate is higher, but production environment requires height, and experimental condition is harsh, and culture medium cost is high, and then causes that the production cost of original silkworm egg is high.
Patent Office of the People's Republic of China discloses " a kind of method that purple sweep potato and hole tray are numerous soon " publication number 104782487A on July 22nd, 2015, the method sterilisation step is: cut 2cm footpath point from purple sweep potato seedling, remove blade, tap water rinses 5-10 minute repeatedly, add a little cleanser essence to soak 10 minutes, clean 4-5 time with distilled water, footpath point is moved on superclean bench, by 75% soak with ethanol 30 seconds, by sterile water wash 2-3 time, again footpath point is placed in the NaCl0 solution of 2% and soaks 10 minutes, or again by sterile water wash after the mercuric chloride solution of 0.1% soaks 5 minutes, footpath point aseptic filter paper after sterilization sucks surface moisture, put in sterile petri dish standby;The footpath merismatic stripping of point of the method with inoculation operation is: be placed in by the footpath point of sterilization under the microscope of 20-80 times, spire is gradually removed with dissecting needle, until exposing smooth footpath point separate living tissue, cut the growing point with the long phyllopodium of 1-2 0.1-0.3 millimeter with dissecting knife;With Inoculating needle, growing point is moved to equipped with cultivation in the culture dish of regeneration culture medium, 20 growing points of inoculation in each culture dish;The cultivation operation of the method is: postvaccinal culture dish is placed on the illumination cultivation frame of illumination box or group training indoor, condition of culture is temperature 25 degree, intensity of illumination 2000 ~ 3000lx, light application time 16h/d, cultivate 20-30d see the aobvious growth of footpath JIANMING and present green, being proceeded to by path bud when having lobule to occur in the test tube of subculture medium and cultivate, continued growth also forms root system, then through the plantlet that can develop into 4 ~ 5 blades for about month;The method also includes hole tray and substrate preparation, detoxicating cuvette transplantation of seedlings, warmhouse booth are cultivated, cut the operations such as Seedling, and operation is loaded down with trivial details, cost is high.
Summary of the invention
The purpose of the present invention is for the weak point of the propagation method of existing Rhizoma Steudnerae Henryanae detoxic seedling, it is provided that the water planting quick-breeding method of a kind of fast Rhizoma Steudnerae Henryanae detoxic seedling that numerous operation is simple, production cost is low.
The technical scheme is that and be achieved in that:
1, sterilization: cut the stem apex of the long Rhizoma Steudnerae Henryanae detoxic seedling of 2.0cm~3.0cm, be fixed in the hole of cystosepiment with sponge or cotton mass, float in the carbendazim solution of 1 ‰, after soaking root 30min, rinses 3 ~ 5 times with clear water;
2, take root: by Rhizoma Steudnerae Henryanae stem apex sterilized for root, put into water planting seedlings nursing plate stands to swim in and take root in nutritional solution, cultivate 5~7d when temperature 22 DEG C~26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%~75%, complete to take root;
3, cultivate: the nutritional solution of taking root in water planting groove is replaced by nutritional solution in strong sprout, being stood by the Seedling of taking root of step (1) swims in nutritional solution in strong sprout, cultivate 12~15d when temperature 22 DEG C~26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%~75%, grow up to after 4~5 leaves as female Seedling;
4, female Seedling step (3) cultivated is cut the stem apex of 2.0cm~3.0cm length, carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate, female Seedling is done again when Seedling grows to 4~5 leaves, cut stem apex again, then carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate;
5, after female Seedling of step (4) is cut stem apex 8~10 times, plant height 5cm~8cm, the thick 5mm~8mm of stem, there is the vigorous Seedling of 7~9 leaves, root system as kind of a transplantation of seedlings, produce original silkworm egg Seedling and potato seed.
There is advantages that
1, the inventive method produces under open environment, and production environment is less demanding;
2, the nutritional solution cost that the present invention uses is low;
3, operation of the present invention is simple, convenient operation;
4, week is short, survival rate is high to use the inventive method to produce;
5, the present invention can greatly reduce the cost producing Rhizoma Steudnerae Henryanae detoxic seedling on the whole;
6, using the Rhizoma Steudnerae Henryanae tissue cultural seedlings of free of the inventive method breeding, root system is vigorous, the thick plant height of stem, transplanting survival rate high, knot potato is many.
Detailed description of the invention
The specific embodiment of the present invention is as follows:
Step 1: sterilization, cuts the stem apex of the long Rhizoma Steudnerae Henryanae detoxic seedling of 2.0cm~3.0cm, is fixed in the hole of cystosepiment with sponge or cotton mass, float in the carbendazim solution of 1 ‰, after soaking root 30min, rinses 3 ~ 5 times with clear water.
Step 2: root culture, the seeding row spacing of cuttage is 5cm × 6cm, putting into stand to swim in water planting seedlings nursing plate takes root in nutritional solution, take root the deep 5cm~6cm of nutritional solution liquid, 5~7d is cultivated when temperature 22 DEG C~26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%~75%, completing to take root, the formula of nutritional solution of taking root is: (NH4)2SO40.0~20/L;KNO30.0~2.5/L;K2SO40.0~2.5/L;MgSO40.0~15.0/L;KH2PO40.0 ~ 3.0/L;Ca (NO3)20.0 ~ 2.0/L;FeSO40.0 ~ 1.0/L;Na2-EDTA0.0 ~ 1.0/L;H3BO30.0 ~ 3.0/L;MnCl20.0 ~ 0.5/L;ZnSO40.0 ~ 0.03/L;CuSO40.0 ~ 0.02/L;Na2MoO40.0~0.02㎎/L。
Nutritional solution of taking root adopts sterilized water preparation.
Step 3: strong seedling culture, nutritional solution of taking root in water planting groove is replaced by nutritional solution in strong sprout, strong sprout the deep 5cm~6cm of nutritional solution liquid, being stood by the Seedling of step 2 swims in nutritional solution in strong sprout, cultivate 12~15d when temperature 22 DEG C~26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%~75%, grow up to after 4~5 leaves as female Seedling.Strong sprout nutritional solution formula:
(NH4)2SO450~150/L;KNO320~80/L;K2SO410~50/L;MgSO450~150/L;KH2PO410 ~ 40/L;Ca (NO3)240 ~ 80/L;FeSO45 ~ 15/L;Na2-EDTA5 ~ 15/L;H3BO32.0 ~ 10.0/L;MnCl21.0 ~ 5.0/L;ZnSO40.10 ~ 0.50/L;
CuSO40.05 ~ 0.20/L;Na2MoO40.05 ~ 0.50/L.Strong sprout, nutritional solution adopted sterilized water preparation.
Step 4: cut stem apex on female Seedling of cultivation, carry out disinfection by the mode of step 1, step 2, take root and cultivate, does again female Seedling when Seedling grows to 4~5 leaves, then on cut stem apex, then carry out disinfection by the mode of step 1, step 2 sum, take root and cultivate.
Step 5:: after mother's Seedling is cut stem apex 8~10 times, plant height 5cm~8cmm, the thick 5mm~8mm of stem, there is the vigorous Seedling of 7~9 leaves, root system as kind of a transplantation of seedlings, from cystosepiment, uproop transplanting in net canopy, produce original silkworm egg potato or seedling.

Claims (3)

1. the method that Rhizoma Steudnerae Henryanae detoxic seedling water planting is numerous soon, including sterilizing, take root, the step such as cultivation, is characterized in that:
(1) sterilization: cut the stem apex of the long Rhizoma Steudnerae Henryanae detoxic seedling of 2.0cm~3.0cm, be fixed in the hole of cystosepiment with sponge or cotton mass, float in the carbendazim solution of 1 ‰, after soaking root 30min, rinses 3 ~ 5 times with clear water;
(2) take root: by Rhizoma Steudnerae Henryanae stem apex sterilized for root, put into water planting seedlings nursing plate stands to swim in and take root in nutritional solution, cultivate 5~7d when temperature 22 DEG C~26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%~75%, complete to take root;
(3) cultivate: the nutritional solution of taking root in water planting groove is replaced by nutritional solution in strong sprout, being stood by the Seedling of taking root of step (1) swims in nutritional solution in strong sprout, cultivate 12~15d when temperature 22 DEG C~26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%~75%, grow up to after 4~5 leaves as female Seedling;
(4) female Seedling step (3) cultivated is cut the stem apex of 2.0cm~3.0cm length, carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate, female Seedling is done again when Seedling grows to 4~5 leaves, cut stem apex again, then carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate;
(5) after female Seedling of step (4) is cut stem apex 8~10 times, plant height 5cm~8cm, the thick 5mm~8mm of stem, there is the vigorous Seedling of 7~9 leaves, root system as kind of a transplantation of seedlings, produce original silkworm egg Seedling and potato seed.
2. Rhizoma Steudnerae Henryanae detoxic seedling water planting quick-breeding method according to claim 1, is characterized in that the formula of described nutritional solution of taking root is:
(NH4)2SO40.0~20/L;KNO30.0~2.5/L;K2SO40.0~2.5/L;MgSO40.0~15.0/L;KH2PO40.0 ~ 3.0/L;Ca (NO3)20.0 ~ 2.0/L;FeSO40.0 ~ 1.0/L;Na2-EDTA0.0 ~ 1.0/L;H3BO30.0 ~ 3.0/L;MnCl20.0 ~ 0.5/L;ZnSO40.0 ~ 0.03/L;CuSO40.0 ~ 0.02/L;Na2MoO40.0 ~ 0.02/L, nutritional solution of taking root adopts sterilized water preparation.
3. Rhizoma Steudnerae Henryanae detoxic seedling water planting quick-breeding method according to claim 1, is characterized in that the formula of nutritional solution in described strong sprout is:
(NH4)2SO450~150/L;KNO320~80/L;K2SO410~50/L;MgSO450~150/L;KH2PO410 ~ 40/L;Ca (NO3)240 ~ 80/L;FeSO45 ~ 15/L;Na2-EDTA5 ~ 15/L;H3BO32.0 ~ 10.0/L;MnCl21.0 ~ 5.0/L;ZnSO40.10 ~ 0.50/L;CuSO40.05 ~ 0.20/L;Na2MoO40.05 ~ 0.50/L, strong sprout, nutritional solution adopted sterilized water preparation.
CN201610144339.8A 2016-03-15 2016-03-15 Purple sweet potato virus-free seedling water culture fast breeding method Pending CN105706880A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610144339.8A CN105706880A (en) 2016-03-15 2016-03-15 Purple sweet potato virus-free seedling water culture fast breeding method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610144339.8A CN105706880A (en) 2016-03-15 2016-03-15 Purple sweet potato virus-free seedling water culture fast breeding method

Publications (1)

Publication Number Publication Date
CN105706880A true CN105706880A (en) 2016-06-29

Family

ID=56157773

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610144339.8A Pending CN105706880A (en) 2016-03-15 2016-03-15 Purple sweet potato virus-free seedling water culture fast breeding method

Country Status (1)

Country Link
CN (1) CN105706880A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106105776A (en) * 2016-08-20 2016-11-16 刘刚 A kind of Pleurotus geesteranus soilless culture method
CN106258880A (en) * 2016-08-08 2017-01-04 湖南农业大学 The floating cuttage of fruit of a kind of band is cultivated small potted plant and is born fruit the method for auspicious fruit
CN106358658A (en) * 2016-08-26 2017-02-01 颍上县凯旋食品有限公司 Highly-efficient seedling-raising method for purple sweet potato
CN106508644A (en) * 2016-10-27 2017-03-22 南充市农业科学院 Sweet potato virus-free breeding method
CN107347608A (en) * 2017-08-22 2017-11-17 西南大学 Virus-free seed potato and seedling water planting integration high power mating system
CN107624583A (en) * 2017-10-20 2018-01-26 山西省农业科学院玉米研究所 The method of the organic Sweet Potato For High. yield Production cultivation in hills drought-hit area

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20000046082A (en) * 1998-12-31 2000-07-25 한동휴 Method for producing potato saplings grown up from stem cutting for growing in liquid nutrition medium and culture broth compositions(cheju b liquid medium)
KR100570833B1 (en) * 2005-10-31 2006-04-13 괴산군 Mass-production method for seedling of seed potato
CN103563748A (en) * 2013-11-01 2014-02-12 四川省植物工程研究院 Method for culturing strong seedling of potato virus-free tube seedling
CN103787722A (en) * 2014-02-14 2014-05-14 徐州市农业科学院 Sweet potato water culture nutrient solution formula for winter greenhouse stem tip vegetables and usage method thereof,
CN103960134A (en) * 2014-06-03 2014-08-06 恩施土家族苗族自治州农业科学院 Method for producing sweet potato detoxified seedlings in water culture manner
CN104211462A (en) * 2013-05-30 2014-12-17 天津职业技术师范大学 Cabbage water planting nutrient solution
CN104211486A (en) * 2013-05-30 2014-12-17 天津职业技术师范大学 Capsicum water planting nutrient solution
CN104304031A (en) * 2014-11-05 2015-01-28 丽江伯符农业科技发展有限公司 Aquaculture rapid propagation method for potato virus-free tissue culture plantlets
CN104782487A (en) * 2015-04-28 2015-07-22 江苏省农业科学院 Detoxification and cell pan rapid propagation method for purple sweet potatoes

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20000046082A (en) * 1998-12-31 2000-07-25 한동휴 Method for producing potato saplings grown up from stem cutting for growing in liquid nutrition medium and culture broth compositions(cheju b liquid medium)
KR100570833B1 (en) * 2005-10-31 2006-04-13 괴산군 Mass-production method for seedling of seed potato
CN104211462A (en) * 2013-05-30 2014-12-17 天津职业技术师范大学 Cabbage water planting nutrient solution
CN104211486A (en) * 2013-05-30 2014-12-17 天津职业技术师范大学 Capsicum water planting nutrient solution
CN103563748A (en) * 2013-11-01 2014-02-12 四川省植物工程研究院 Method for culturing strong seedling of potato virus-free tube seedling
CN103787722A (en) * 2014-02-14 2014-05-14 徐州市农业科学院 Sweet potato water culture nutrient solution formula for winter greenhouse stem tip vegetables and usage method thereof,
CN103960134A (en) * 2014-06-03 2014-08-06 恩施土家族苗族自治州农业科学院 Method for producing sweet potato detoxified seedlings in water culture manner
CN104304031A (en) * 2014-11-05 2015-01-28 丽江伯符农业科技发展有限公司 Aquaculture rapid propagation method for potato virus-free tissue culture plantlets
CN104782487A (en) * 2015-04-28 2015-07-22 江苏省农业科学院 Detoxification and cell pan rapid propagation method for purple sweet potatoes

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106258880A (en) * 2016-08-08 2017-01-04 湖南农业大学 The floating cuttage of fruit of a kind of band is cultivated small potted plant and is born fruit the method for auspicious fruit
CN106105776A (en) * 2016-08-20 2016-11-16 刘刚 A kind of Pleurotus geesteranus soilless culture method
CN106358658A (en) * 2016-08-26 2017-02-01 颍上县凯旋食品有限公司 Highly-efficient seedling-raising method for purple sweet potato
CN106508644A (en) * 2016-10-27 2017-03-22 南充市农业科学院 Sweet potato virus-free breeding method
CN106508644B (en) * 2016-10-27 2020-05-15 南充市农业科学院 Sweet potato virus-free seed potato propagation method
CN107347608A (en) * 2017-08-22 2017-11-17 西南大学 Virus-free seed potato and seedling water planting integration high power mating system
CN107624583A (en) * 2017-10-20 2018-01-26 山西省农业科学院玉米研究所 The method of the organic Sweet Potato For High. yield Production cultivation in hills drought-hit area

Similar Documents

Publication Publication Date Title
CN105706880A (en) Purple sweet potato virus-free seedling water culture fast breeding method
CN104429971B (en) Tissue culture seedling-raising method of millettia fordii dunn
CN102422816A (en) Method for rapidly culturing shoot tips in vitro
CN101116424B (en) Highly effective lily bulblet inducement culture method
CN104585036B (en) The tissue culture and rapid propagation method of the fiery axillalry bud on Malus spectabilis plateau, a kind of North America
CN103651141B (en) The method that Bo chrysanthemum batch production test tube seedling is the most numerous
CN106718883A (en) A kind of Yunnan Chinese catalpa and the miniature engrafting method of test tube seedling of Chinese catalpa
CN103704130A (en) Chinese orchid and cymbidium hybridum hybrid seedling raising method
CN100391333C (en) Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum
CN110402818B (en) Tissue culture and rapid propagation seedling raising method for mature embryos of high-quality Chinese chestnuts
CN106665357A (en) Method for establishing lycoris regeneration system
CN107371880A (en) A kind of apple rootstock tissue culturing fast seedling-cultivating method
CN103125393B (en) Aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am
CN106613960A (en) Rapid propagation method of paphiopedilum helenae callus regeneration system
CN103155869A (en) Sweet cherry rootstock Colt tissue culture method
CN104604683A (en) Rapid propagation method for ottelia acuminata seedlings by tissue culture
CN103168692B (en) Salix saposhnikovii tissue culture method
CN107155882A (en) A kind of medicinal bletilla striata aseptic seeding fast seedling-cultivating method
CN106465680B (en) Rapid celery tissue culture system
CN102232359B (en) In-vitro rapid propagation method of double-petal Jasminum sambac
CN103704140A (en) Method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants
CN101595843A (en) Kuh-seng tissue culture and method for quickly breeding
CN104585040A (en) Method for quickly propagating germchit of giantleaf ardisia rhizome through bud plumular axis
CN104322370A (en) Bitter gourd in vitro rapid propagation method
CN109042330A (en) A kind of method for tissue culture of spindle tree

Legal Events

Date Code Title Description
PB01 Publication
C06 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160629

WD01 Invention patent application deemed withdrawn after publication