CN105706880A - Purple sweet potato virus-free seedling water culture fast breeding method - Google Patents
Purple sweet potato virus-free seedling water culture fast breeding method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention provides a purple sweet potato virus-free seedling water culture fast breeding method, and belongs to the field of crop seedling culture. The method comprises the following steps of 1, performing sterilization: shearing sweet potato virus-free seedling stem tops with the length of 2.0cm to 3.0cm; fixing the sweet potato virus-free seedling stem tops into foam plate holes by sponge or cotton; floating the sweet potato virus-free seedling stem tops in a 0.1-percent carbendazim solution for soaking the roots for 30min; then, performing flushing for 3 to 5 times; 2, performing rooting: putting seedlings sterilized in the first step into a water culture seedling culture disc for culturing 5 to 7 days through the still standing floating in a rooting nutrition solution until the water roots grow to 1.0cm to 3.0cm; 3, performing culture: culturing the purple sweet potato seedlings with the water roots in the second step in a strong seedling nutrition liquid for 12 to 15d in a nutrition solution; using the seedlings as mother seedlings when 4 to 5 leaves grow; 4, shearing stem tops with the length being 2.0cm to 3.0cm from the mother seedlings cultured in the third step, and performing sterilization, rooting and culturing; 5, shearing the stem tips on the other seedlings obtained in the third step for 8 to 10 times and then using the mother seedlings as seed seedlings to be transplanted. When the method provided by the invention is used for breeding the purple sweet potato virus-free seedlings, the production cost is low; the quality is high.
Description
Technical field
The invention belongs to crops seedling-raising technique field, particularly the water planting quick-breeding method of a kind of Rhizoma Steudnerae Henryanae detoxic seedling.
Background technology
The modes of reproduction of current Rhizoma Steudnerae Henryanae detoxic seedling mainly has two kinds, and a kind of is that the branch that detoxification original silkworm egg potato grows carries out cottage propagation, and this method repoductive time is long, and survival rate is low, it is easy to bacteria infection, causes that the production cost of original silkworm egg is high；Another kind is to breed in Rhizoma Steudnerae Henryanae detoxification test tube plantlet culture medium based on MS, and this method reproduction speed ratio is very fast, and survival rate is higher, but production environment requires height, and experimental condition is harsh, and culture medium cost is high, and then causes that the production cost of original silkworm egg is high.
Patent Office of the People's Republic of China discloses " a kind of method that purple sweep potato and hole tray are numerous soon " publication number 104782487A on July 22nd, 2015, the method sterilisation step is: cut 2cm footpath point from purple sweep potato seedling, remove blade, tap water rinses 5-10 minute repeatedly, add a little cleanser essence to soak 10 minutes, clean 4-5 time with distilled water, footpath point is moved on superclean bench, by 75% soak with ethanol 30 seconds, by sterile water wash 2-3 time, again footpath point is placed in the NaCl0 solution of 2% and soaks 10 minutes, or again by sterile water wash after the mercuric chloride solution of 0.1% soaks 5 minutes, footpath point aseptic filter paper after sterilization sucks surface moisture, put in sterile petri dish standby；The footpath merismatic stripping of point of the method with inoculation operation is: be placed in by the footpath point of sterilization under the microscope of 20-80 times, spire is gradually removed with dissecting needle, until exposing smooth footpath point separate living tissue, cut the growing point with the long phyllopodium of 1-2 0.1-0.3 millimeter with dissecting knife；With Inoculating needle, growing point is moved to equipped with cultivation in the culture dish of regeneration culture medium, 20 growing points of inoculation in each culture dish；The cultivation operation of the method is: postvaccinal culture dish is placed on the illumination cultivation frame of illumination box or group training indoor, condition of culture is temperature 25 degree, intensity of illumination 2000 ~ 3000lx, light application time 16h/d, cultivate 20-30d see the aobvious growth of footpath JIANMING and present green, being proceeded to by path bud when having lobule to occur in the test tube of subculture medium and cultivate, continued growth also forms root system, then through the plantlet that can develop into 4 ~ 5 blades for about month；The method also includes hole tray and substrate preparation, detoxicating cuvette transplantation of seedlings, warmhouse booth are cultivated, cut the operations such as Seedling, and operation is loaded down with trivial details, cost is high.
Summary of the invention
The purpose of the present invention is for the weak point of the propagation method of existing Rhizoma Steudnerae Henryanae detoxic seedling, it is provided that the water planting quick-breeding method of a kind of fast Rhizoma Steudnerae Henryanae detoxic seedling that numerous operation is simple, production cost is low.
The technical scheme is that and be achieved in that:
1, sterilization: cut the stem apex of the long Rhizoma Steudnerae Henryanae detoxic seedling of 2.0cm～3.0cm, be fixed in the hole of cystosepiment with sponge or cotton mass, float in the carbendazim solution of 1 ‰, after soaking root 30min, rinses 3 ~ 5 times with clear water；
2, take root: by Rhizoma Steudnerae Henryanae stem apex sterilized for root, put into water planting seedlings nursing plate stands to swim in and take root in nutritional solution, cultivate 5～7d when temperature 22 DEG C～26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%～75%, complete to take root；
3, cultivate: the nutritional solution of taking root in water planting groove is replaced by nutritional solution in strong sprout, being stood by the Seedling of taking root of step (1) swims in nutritional solution in strong sprout, cultivate 12～15d when temperature 22 DEG C～26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%～75%, grow up to after 4～5 leaves as female Seedling；
4, female Seedling step (3) cultivated is cut the stem apex of 2.0cm～3.0cm length, carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate, female Seedling is done again when Seedling grows to 4～5 leaves, cut stem apex again, then carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate；
5, after female Seedling of step (4) is cut stem apex 8～10 times, plant height 5cm～8cm, the thick 5mm～8mm of stem, there is the vigorous Seedling of 7～9 leaves, root system as kind of a transplantation of seedlings, produce original silkworm egg Seedling and potato seed.
There is advantages that
1, the inventive method produces under open environment, and production environment is less demanding；
2, the nutritional solution cost that the present invention uses is low；
3, operation of the present invention is simple, convenient operation；
4, week is short, survival rate is high to use the inventive method to produce；
5, the present invention can greatly reduce the cost producing Rhizoma Steudnerae Henryanae detoxic seedling on the whole；
6, using the Rhizoma Steudnerae Henryanae tissue cultural seedlings of free of the inventive method breeding, root system is vigorous, the thick plant height of stem, transplanting survival rate high, knot potato is many.
Detailed description of the invention
The specific embodiment of the present invention is as follows:
Step 1: sterilization, cuts the stem apex of the long Rhizoma Steudnerae Henryanae detoxic seedling of 2.0cm～3.0cm, is fixed in the hole of cystosepiment with sponge or cotton mass, float in the carbendazim solution of 1 ‰, after soaking root 30min, rinses 3 ~ 5 times with clear water.
Step 2: root culture, the seeding row spacing of cuttage is 5cm × 6cm, putting into stand to swim in water planting seedlings nursing plate takes root in nutritional solution, take root the deep 5cm～6cm of nutritional solution liquid, 5～7d is cultivated when temperature 22 DEG C～26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%～75%, completing to take root, the formula of nutritional solution of taking root is: (NH4)2SO40.0～20/L；KNO30.0～2.5/L；K2SO40.0～2.5/L；MgSO40.0～15.0/L；KH2PO40.0 ~ 3.0/L；Ca (NO3)20.0 ~ 2.0/L；FeSO40.0 ~ 1.0/L；Na2-EDTA0.0 ~ 1.0/L；H3BO30.0 ~ 3.0/L；MnCl20.0 ~ 0.5/L；ZnSO40.0 ~ 0.03/L；CuSO40.0 ~ 0.02/L；Na2MoO40.0~0.02㎎/L。
Nutritional solution of taking root adopts sterilized water preparation.
Step 3: strong seedling culture, nutritional solution of taking root in water planting groove is replaced by nutritional solution in strong sprout, strong sprout the deep 5cm～6cm of nutritional solution liquid, being stood by the Seedling of step 2 swims in nutritional solution in strong sprout, cultivate 12～15d when temperature 22 DEG C～26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%～75%, grow up to after 4～5 leaves as female Seedling.Strong sprout nutritional solution formula:
(NH4)2SO450～150/L；KNO320～80/L；K2SO410～50/L；MgSO450～150/L；KH2PO410 ~ 40/L；Ca (NO3)240 ~ 80/L；FeSO45 ~ 15/L；Na2-EDTA5 ~ 15/L；H3BO32.0 ~ 10.0/L；MnCl21.0 ~ 5.0/L；ZnSO40.10 ~ 0.50/L；
CuSO40.05 ~ 0.20/L；Na2MoO40.05 ~ 0.50/L.Strong sprout, nutritional solution adopted sterilized water preparation.
Step 4: cut stem apex on female Seedling of cultivation, carry out disinfection by the mode of step 1, step 2, take root and cultivate, does again female Seedling when Seedling grows to 4～5 leaves, then on cut stem apex, then carry out disinfection by the mode of step 1, step 2 sum, take root and cultivate.
Step 5:: after mother's Seedling is cut stem apex 8～10 times, plant height 5cm～8cmm, the thick 5mm～8mm of stem, there is the vigorous Seedling of 7～9 leaves, root system as kind of a transplantation of seedlings, from cystosepiment, uproop transplanting in net canopy, produce original silkworm egg potato or seedling.
Claims (3)
1. the method that Rhizoma Steudnerae Henryanae detoxic seedling water planting is numerous soon, including sterilizing, take root, the step such as cultivation, is characterized in that:
(1) sterilization: cut the stem apex of the long Rhizoma Steudnerae Henryanae detoxic seedling of 2.0cm～3.0cm, be fixed in the hole of cystosepiment with sponge or cotton mass, float in the carbendazim solution of 1 ‰, after soaking root 30min, rinses 3 ~ 5 times with clear water；
(2) take root: by Rhizoma Steudnerae Henryanae stem apex sterilized for root, put into water planting seedlings nursing plate stands to swim in and take root in nutritional solution, cultivate 5～7d when temperature 22 DEG C～26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%～75%, complete to take root；
(3) cultivate: the nutritional solution of taking root in water planting groove is replaced by nutritional solution in strong sprout, being stood by the Seedling of taking root of step (1) swims in nutritional solution in strong sprout, cultivate 12～15d when temperature 22 DEG C～26 DEG C, intensity of illumination are 7200lx, illumination 12h/d, humidity 70%～75%, grow up to after 4～5 leaves as female Seedling；
(4) female Seedling step (3) cultivated is cut the stem apex of 2.0cm～3.0cm length, carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate, female Seedling is done again when Seedling grows to 4～5 leaves, cut stem apex again, then carry out disinfection by the mode of step (1), step (2) and step (3), take root and cultivate；
(5) after female Seedling of step (4) is cut stem apex 8～10 times, plant height 5cm～8cm, the thick 5mm～8mm of stem, there is the vigorous Seedling of 7～9 leaves, root system as kind of a transplantation of seedlings, produce original silkworm egg Seedling and potato seed.
2. Rhizoma Steudnerae Henryanae detoxic seedling water planting quick-breeding method according to claim 1, is characterized in that the formula of described nutritional solution of taking root is:
(NH4)2SO40.0～20/L；KNO30.0～2.5/L；K2SO40.0～2.5/L；MgSO40.0～15.0/L；KH2PO40.0 ~ 3.0/L；Ca (NO3)20.0 ~ 2.0/L；FeSO40.0 ~ 1.0/L；Na2-EDTA0.0 ~ 1.0/L；H3BO30.0 ~ 3.0/L；MnCl20.0 ~ 0.5/L；ZnSO40.0 ~ 0.03/L；CuSO40.0 ~ 0.02/L；Na2MoO40.0 ~ 0.02/L, nutritional solution of taking root adopts sterilized water preparation.
3. Rhizoma Steudnerae Henryanae detoxic seedling water planting quick-breeding method according to claim 1, is characterized in that the formula of nutritional solution in described strong sprout is:
(NH4)2SO450～150/L；KNO320～80/L；K2SO410～50/L；MgSO450～150/L；KH2PO410 ~ 40/L；Ca (NO3)240 ~ 80/L；FeSO45 ~ 15/L；Na2-EDTA5 ~ 15/L；H3BO32.0 ~ 10.0/L；MnCl21.0 ~ 5.0/L；ZnSO40.10 ~ 0.50/L；CuSO40.05 ~ 0.20/L；Na2MoO40.05 ~ 0.50/L, strong sprout, nutritional solution adopted sterilized water preparation.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106105776A (en) * | 2016-08-20 | 2016-11-16 | 刘刚 | A kind of Pleurotus geesteranus soilless culture method |
| CN106258880A (en) * | 2016-08-08 | 2017-01-04 | 湖南农业大学 | The floating cuttage of fruit of a kind of band is cultivated small potted plant and is born fruit the method for auspicious fruit |
| CN106358658A (en) * | 2016-08-26 | 2017-02-01 | 颍上县凯旋食品有限公司 | Highly-efficient seedling-raising method for purple sweet potato |
| CN106508644A (en) * | 2016-10-27 | 2017-03-22 | 南充市农业科学院 | Sweet potato virus-free breeding method |
| CN107347608A (en) * | 2017-08-22 | 2017-11-17 | 西南大学 | Virus-free seed potato and seedling water planting integration high power mating system |
| CN107624583A (en) * | 2017-10-20 | 2018-01-26 | 山西省农业科学院玉米研究所 | The method of the organic Sweet Potato For High. yield Production cultivation in hills drought-hit area |
| CN115812598A (en) * | 2022-12-07 | 2023-03-21 | 江苏丰收大地种业发展有限公司 | Liquid culture method for sweet potato detoxified tissue culture seedlings by taking sponge as support |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258880A (en) * | 2016-08-08 | 2017-01-04 | 湖南农业大学 | The floating cuttage of fruit of a kind of band is cultivated small potted plant and is born fruit the method for auspicious fruit |
| CN106105776A (en) * | 2016-08-20 | 2016-11-16 | 刘刚 | A kind of Pleurotus geesteranus soilless culture method |
| CN106358658A (en) * | 2016-08-26 | 2017-02-01 | 颍上县凯旋食品有限公司 | Highly-efficient seedling-raising method for purple sweet potato |
| CN106508644A (en) * | 2016-10-27 | 2017-03-22 | 南充市农业科学院 | Sweet potato virus-free breeding method |
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| CN107347608A (en) * | 2017-08-22 | 2017-11-17 | 西南大学 | Virus-free seed potato and seedling water planting integration high power mating system |
| CN107624583A (en) * | 2017-10-20 | 2018-01-26 | 山西省农业科学院玉米研究所 | The method of the organic Sweet Potato For High. yield Production cultivation in hills drought-hit area |
| CN115812598A (en) * | 2022-12-07 | 2023-03-21 | 江苏丰收大地种业发展有限公司 | Liquid culture method for sweet potato detoxified tissue culture seedlings by taking sponge as support |
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