CN115812598A - Liquid culture method for sweet potato detoxified tissue culture seedlings by taking sponge as support - Google Patents
Liquid culture method for sweet potato detoxified tissue culture seedlings by taking sponge as support Download PDFInfo
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- CN115812598A CN115812598A CN202211567534.3A CN202211567534A CN115812598A CN 115812598 A CN115812598 A CN 115812598A CN 202211567534 A CN202211567534 A CN 202211567534A CN 115812598 A CN115812598 A CN 115812598A
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Abstract
The invention discloses a liquid culture method of a sweet potato detoxified tissue culture seedling by taking sponge as a support, which comprises the following steps: s1, sponge processing: processing sponge into sponge blocks, and forming holes matched with the diameter of the stem sections of the sweet potato virus-free tissue culture seedlings in the middle of the sponge blocks; s2, sweet potato virus-free tissue culture seedling micro-cuttage: when the sweet potato detoxified tissue culture seedlings are subjected to micro cuttage, the stem sections of the sweet potato detoxified tissue culture seedlings are inserted into the sponge blocks, and liquid proliferation nutrient solution or rooting nutrient solution is poured into the sponge blocks for proliferation culture or rooting culture. By taking the perforated sponge as a support, the cost of the culture medium is greatly reduced, the production process is reduced, and the tissue culture propagation coefficient and the production efficiency are improved; the nutrient solution is adopted for micro-cuttage liquid culture, so that the use of agar is saved, the cost of the culture medium is greatly reduced, meanwhile, due to the good liquidity of the nutrient solution, the nutrient components fully flow, so that the sweet potato virus-free tissue culture seedlings can more easily and fully absorb nutrients, the growth is better, and the use amount of the culture medium is also reduced.
Description
Technical Field
The invention belongs to the technical field of agricultural planting, and particularly relates to a liquid culture method for a sweet potato detoxified tissue culture seedling by taking sponge as a support.
Background
Sweet potatoes are one of four large food crops in China, the perennial planting area is 7-8 million mu, the yield is over hundred million tons, the sweet potatoes account for 60 percent of the planting area all over the world, and the yield is 80 percent. Because sweet potatoes have the reputation of longevity foods and anticancer foods, people have greater and greater requirements on high-quality sweet potatoes, and the planting benefits of farmers are better and better.
Sweet potatoes are annual or perennial plants of the Convolvulaceae family, are mainly subjected to generation alternation through asexual propagation, and are infected by more than 10 viruses in the growing process, so that the yield and the quality are reduced year by year, and the variety is seriously degraded. By stripping the growing points of the sweet potato varieties for tissue culture, plants which are not infected by viruses can be obtained, and the purposes of purification and rejuvenation of the varieties are achieved. Therefore, the method for culturing the stem tip meristem and massively propagating the virus-free seedlings becomes the best method for producing the virus-free sweet potato seedlings at present.
The detoxification of sweet potatoes comprises the steps of firstly culturing meristematic cells which are not infected by viruses from a stem tip growing point, extracting the whole RNA of the sweet potatoes when the seedlings grow into test tube tissue culture seedlings, carrying out virus detection by using a PCR method, selecting tissue culture seedlings which are identified to be free of viruses for rapid propagation, then carrying out rooting culture on the tissue culture seedlings, then placing the rooted tissue culture seedlings into a greenhouse for field planting to a substrate for domestication, and finally selling the domesticated plug seedlings to a seedling breeding special household for producing the detoxified sweet potatoes.
At present, the rapid propagation and rooting culture of detoxified sweet potato tissue culture seedlings adopts an agar-containing propagation culture medium and a rooting culture medium, a solid culture medium formed by agar is used as a support of a plant for micro-cuttage propagation or rooting, namely, a test-tube seedling is cut into stem sections with 1-2 leaves and directly inserted into the culture medium, nutrients in the culture medium are used for the plant to grow or root, and when the plant grows to about 10cm, micro-cuttage is repeated, so that the purpose of rapid propagation or rooting is realized.
However, the above method has two problems: 1. agar has high cost, which accounts for 60 percent of the cost of the whole culture medium; 2. when the micro-cuttage method is adopted for rapid propagation, after the stem sections taken out of the upper part are subjected to micro-cuttage, because the nutrition around the root system of the plant in the solid culture medium (agar culture medium) is basically exhausted, the old seedlings (old roots) are difficult to sprout again, so that the old seedlings are eliminated, and the propagation coefficient is relatively low; 3. when the rooted seedlings are acclimatized and fixedly planted to the matrix, the agar culture medium is washed off, the seedlings can be planted in the matrix for acclimatization, otherwise, the culture medium can cause the growth of mould to cause the death of tissue culture seedlings, and therefore, the acclimatization step consumes a large amount of labor.
Therefore, a new liquid culture method for the detoxified tissue culture seedlings of sweet potatoes is needed.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a liquid culture method of sweet potato detoxified tissue culture seedlings by taking sponge as a support, which reduces the cost of a culture medium, reduces the production process and improves the tissue culture propagation coefficient and the production efficiency.
In order to solve the technical problems, the invention adopts the technical scheme that: the liquid culture method of the sweet potato detoxified tissue culture seedling with sponge as a support comprises the following steps:
s1, sponge processing: processing sponge into sponge blocks, and forming holes matched with the diameter of the stem sections of the sweet potato virus-free tissue culture seedlings in the middle of the sponge blocks;
s2, liquid micro-cuttage culture of the sweet potato virus-free tissue culture seedlings: when the sweet potato detoxified tissue culture seedlings are subjected to micro cuttage, the stem sections of the sweet potato detoxified tissue culture seedlings are inserted into the sponge blocks, and liquid proliferation nutrient solution or rooting nutrient solution is poured into the sponge blocks for proliferation culture or rooting culture.
According to the invention, the perforated sponge is used as a support to carry out detoxified sweet potato tissue culture seedling nutrient solution enrichment culture and rooting culture, so that the cost of a culture medium is greatly reduced, the production process is reduced, and the tissue culture propagation coefficient and the production efficiency are improved; the sponge has the functions of water absorption, moisture retention and ventilation, and the sponge is used as a support for micro-cuttage propagation and rooting culture of tissue culture seedlings, so that required nutrients, moisture and air can be supplied to the tissue culture seedlings uninterruptedly, the supporting effect of an agar solid culture medium is replaced, the nutrient solution can be supplemented, the continuous culture of old seedlings is realized, and the propagation coefficient is improved; the nutrient solution is adopted for micro-cuttage liquid culture, so that the use of agar is saved, the cost of the culture medium is greatly reduced, and meanwhile, as the fluidity of the nutrient solution is good, the MS concentration in the nutrient solution is reduced by half to obtain the same culture effect, the comprehensive cost of the culture medium is reduced by more than 65 percent (energy and manual calculation); the liquid culture method has the advantages that the operation flow of agar dissolution is omitted, the working efficiency is improved, the nutrition is more uniform, the nutrients are sufficiently flowed, so that sweet potato virus-free tissue culture seedlings can more easily and sufficiently absorb the nutrients, the growth is better, and the using amount of the culture medium is correspondingly reduced.
Preferably, in the step S2, performing micro-cuttage propagation culture, when the sweet potato tissue culture seedling grows to about 10cm high and 5-6 leaves, cutting the sweet potato tissue culture seedling according to 1-2 leaves/stem segment, and inserting the sweet potato tissue culture seedling into the holes of 4-5 new sponge squares for propagation culture, wherein the stem segment length is 1.5-2 cm;
and adding new liquid proliferation nutrient solution into the culture bottle of the old seedling, and growing and propagating the old seedling again after obtaining the new nutrient solution.
Preferably, in the step S2, when the micro cutting propagation culture is performed, the method includes the following steps:
s2-1: the liquid proliferation nutrient solution comprises 1/2MS, 6-BA0.1mg/L and 3% of cane sugar, the pH value is 5.8, and agar powder is not added; adding 30mL of liquid proliferation nutrient solution into a culture bottle, placing 6-8 sponge squares punched in the step S1 into the culture bottle, and inoculating seedlings after high-temperature sterilization;
s2-2: inoculating, cutting the stem section of the sweet potato virus-free tissue culture seedling into 1-3cm in length, and inserting the stem section with 1-2 leaves into the sponge square hole, wherein 6-8 plants can be inserted into each bottle;
s2-3: culturing at 24-26 deg.C under illumination of 2000lx for 14h/d; in the culture period of 30 days, when the plant grows to be 10cm high and more than 5-6 leaves, the micro-cuttage culture can be repeatedly carried out;
s2-4: micro-cuttage, repeating the step S2-2, cutting the plant into stem sections with the length of 1-3cm and 1-2 leaves, reinserting the stem sections into sponge square holes, and putting the sponge square holes into a culture bottle of liquid proliferation nutrient solution for proliferation culture;
s2-5: and (4) performing proliferation culture on the old seedlings, and adding a new liquid proliferation nutrient solution into a culture bottle of the original mother seedlings to grow again for proliferation culture.
By adopting the technical scheme, the propagation coefficient of the detoxified sweet potato tissue culture seedling can be increased by more than 20%.
Preferably, in the step S2, rooting culture of the detoxified tissue culture micro-cutting seedlings of the sweet potatoes is carried out, liquid rooting nutrient solution is changed into rooting culture in a culture bottle, the rooting seedlings are taken out together with sponge blocks after the detoxified sweet potato tissue culture seedlings take roots and are directly implanted into a matrix, after 1-week heat preservation, moisture preservation and light-proof acclimatization culture, the tissue culture seedlings grow new roots in the matrix to be alive, and after 2-3 weeks growth, the detoxified sweet potato tissue culture acclimatization seedlings can be sold as original seeds.
Because the sponge is used as a support, a solid culture medium carried by the root system of the tissue culture seedling does not need to be cleaned, the domestication operation can be completed by directly rinsing with clear water (500 times of carbendazim solution) and then putting in a substrate, the yield of operators is improved to 2600 plants from 2000 plants domesticated per person per day, and the efficiency is improved by more than 25%; because the sponge has the functions of moisture preservation and ventilation, the survival rate of the tissue culture rooted seedlings during domestication is improved to a certain extent, and the sponge blocks do not influence the later growth of the sweet potato seedlings.
Preferably, in the step S2, when the micro-cutting rooting culture is performed, the method includes the following steps:
s2-1: the liquid rooting nutrient solution comprises 1/2MS and 3% of sucrose, the pH value is 5.8, and agar powder is not added; adding 30mL of liquid rooting nutrient solution into a culture bottle, placing 8-10 sponge blocks punched in the step S1 into the culture bottle, and inoculating seedlings after high-temperature sterilization;
s2-2: inoculating, namely inserting cut stem sections of the sweet potato virus-free tissue culture seedlings into sponge square holes, wherein 8-10 plants can be inserted into each bottle;
s2-3: culturing at 26-28 deg.C under 3000lx for 12/d; culturing for 20-25 days, and acclimating when the root system grows out of the sponge square;
s2-4: and (3) performing rooting culture on the old seedlings, and after the propagation and micro-cuttage culture of the mother seedlings, replacing the mother seedlings in a culture bottle with liquid rooting nutrient solution to perform rooting culture, wherein the old seedlings are used for rooting, so that the number of the rooted seedlings is increased.
Preferably, in the step S2-3, the domestication of the rooted seedlings to the seedlings specifically comprises the following steps:
(1) Soaking a 50-hole plug tray in 0.1% potassium permanganate solution for 30 minutes for later use;
(2) Preparing a domestication substrate: mixing turf and perlite according to a volume ratio of 3;
(3) Rinsing the rooted tissue culture seedling once in 500 times diluted carbendazim solution, and then planting the sponge square with the tissue culture seedling into a matrix;
(4) Building a small arched shed, and covering a film and a sunshade net;
(5) Controlling the temperature to be 20-30 ℃ on the fourth day, and gradually ventilating and exposing; after one week, the film can be taken off for normal management.
Due to the change of ecological environments such as climate and the like and the high-strength replanting state of land, the plant diseases and insect pests of crops become more serious day by day, and the sweet potato virus-free tissue culture seedlings achieve the purpose of purifying and rejuvenating varieties due to virus harm removed by the sweet potato virus-free tissue culture seedlings, so that the sweet potato virus-free tissue culture seedlings show good disease resistance, high yield and high quality, and the market demand on the tissue culture seedlings is increasingly expanded. By improving the tissue culture method, the production efficiency of the virus-free tissue culture can be greatly improved, the cost is reduced, and a technical basis is provided for further industrialization of virus-free seedlings; the method can be used for producing the detoxified tissue culture seedlings of the sweet potatoes and can also be used for reference by other crops with the tissue culture seedlings subjected to micro-cutting propagation and rooting.
The invention has the following advantages: 1. the cost is greatly reduced: besides the cost of the sponge is increased (0.002 yuan per seedling), the culture medium cost can be reduced by 80%, the labor cost is reduced by 25%, the total cost is reduced by more than 65% (including energy and labor), and about 0.07 yuan per seedling; 2. the efficiency is improved: the operation of agar dissolution is omitted, and the efficiency of inoculation and rooting domestication is improved; 3. greatly improving the yield of the proliferated seedlings and the rooted seedlings: because the old seedlings can be added with nutrient solution for culture, the yield of the propagated seedlings and the rooted seedlings can be improved by more than 20 percent. Therefore, the popularization and application of the invention can be a great promotion to the industrialization of the sweet potato virus-free tissue culture seedlings.
Drawings
FIG. 1 is a schematic diagram of a sponge structure in the liquid culture method of sweet potato detoxified tissue culture seedlings with sponge as a support according to the present invention;
FIG. 2 is a structural diagram of rooting culture of detoxified sweet potato tissue culture seedlings in the liquid culture method of detoxified sweet potato tissue culture seedlings with sponge as a support.
Detailed Description
The following embodiments of the present invention are described in detail with reference to the accompanying drawings, and the following embodiments are only used to more clearly illustrate the technical solutions of the present invention, but not to limit the scope of the present invention.
The liquid culture method of the sweet potato detoxified tissue culture seedling with the sponge as the support in the embodiment of the invention comprises the following steps:
s1, sponge processing: processing sponge into sponge blocks, forming holes matched with the diameter of the stem section of the sweet potato virus-free tissue culture seedling in the middle of the sponge blocks, and punching a hole of 2mm in the middle of the sponge block with the square of 1cm as shown in figure 1;
s2, carrying out liquid micro-cuttage culture on the detoxified tissue culture seedlings of sweet potatoes: when the sweet potato detoxified tissue culture seedlings are subjected to micro cuttage, the stem sections of the sweet potato detoxified tissue culture seedlings are inserted into the sponge blocks, and liquid proliferation nutrient solution or rooting nutrient solution is poured into the sponge blocks for proliferation culture or rooting culture.
In the step S2, performing micro-cuttage propagation culture, cutting the sweet potato tissue culture seedlings according to 1-2 leaves/stem section when the sweet potato tissue culture seedlings grow to be about 10cm high and 5-6 leaves, and inserting the cut sweet potato tissue culture seedlings into 4-5 new sponge square block holes for propagation culture, wherein the stem section length is 1.5-2 cm;
and adding new liquid proliferation nutrient solution into the culture bottle of the old seedling, and growing and propagating the old seedling again after obtaining the new nutrient solution.
In the step S2, when the micro cutting propagation culture is performed, the method includes the following steps:
s2-1: the liquid proliferation nutrient solution comprises 1/2MS, 0.1 mg/L6-BA and 3% sucrose, the pH value is 5.8, and agar powder is not added; adding 30mL of liquid proliferation nutrient solution into a culture bottle, placing 6-8 sponge squares punched in the step S1 into the culture bottle, and inoculating seedlings after high-temperature sterilization;
s2-2: inoculating, cutting the stem section of the sweet potato virus-free tissue culture seedling into 1-3cm in length, and inserting the stem section with 1-2 leaves into the sponge square hole, wherein 6-8 plants can be inserted into each bottle;
s2-3: culturing at 24-26 deg.C under illumination of 2000lx for 14h/d; in the culture period of 30 days, when the plant grows to be 10cm high and more than 5-6 leaves, the micro-cuttage culture can be repeatedly carried out;
s2-4: micro-cuttage, namely repeating the step S2-2, cutting the plant into stem sections with the length of 1-3cm and 1-2 leaves, re-inserting the stem sections into the sponge holes, and putting the stem sections into a culture bottle of liquid proliferation nutrient solution for proliferation culture;
s2-5: and (4) performing proliferation culture on the old seedlings, and adding a new liquid proliferation nutrient solution into a culture bottle of the original mother seedlings to grow again for proliferation culture.
In addition, in the step S2, as shown in fig. 2, the sweet potato detoxification tissue culture micro-cutting seedling is subjected to rooting culture, the culture flask is changed into a liquid rooting nutrient solution to be subjected to rooting culture, after the detoxified sweet potato tissue culture seedling roots, the rooting seedling is taken out together with a sponge block and directly implanted into a matrix, after 1 week of heat preservation, moisture preservation and light-resistant domestication culture, the tissue culture seedling grows to form a new root in the matrix, and after 2-3 weeks of growth, the detoxified sweet potato tissue culture domestication seedling can be sold as an original seed;
in the step S2, when the micro-cutting rooting culture is performed, the method includes the following steps:
s2-1: the liquid rooting nutrient solution comprises 1/2MS and 3% of sucrose, the pH value is 5.8, and agar powder is not added; adding 30mL of liquid rooting nutrient solution into a culture bottle, placing 8-10 sponge blocks punched in the step S1 into the culture bottle, and inoculating seedlings after high-temperature sterilization;
s2-2: inoculating, namely inserting cut stem sections of the sweet potato virus-free tissue culture seedlings into sponge square holes, wherein 8-10 plants can be inserted into each bottle;
s2-3: culturing at 26-28 deg.C under 3000lx for 12h/d; culturing for 20-25 days, and acclimating when the root system grows out of the sponge square;
s2-4: and (3) performing rooting culture on the old seedlings, and after the propagation and micro-cuttage culture of the mother seedlings, replacing the mother seedlings in a culture bottle with liquid rooting nutrient solution to perform rooting culture, wherein the old seedlings are used for rooting, so that the number of the rooted seedlings is increased.
In the step S2-3, the domestication of the rooted seedlings into seedlings specifically comprises the following steps:
(1) Soaking a 50-hole plug tray in 0.1% potassium permanganate solution for 30 minutes for later use;
(2) Preparing a domestication substrate: mixing turf and perlite according to a volume ratio of 3;
(3) Rinsing the rooted tissue culture seedling in 500 times diluted carbendazim solution once, and then planting the sponge square with the tissue culture seedling into a matrix;
(4) Building a small arched shed, and covering a film and a sunshade net;
(5) Controlling the temperature to be 20-30 ℃ on the fourth day, and gradually ventilating and exposing; after one week, the film can be lifted for normal management.
According to the liquid culture method for the sweet potato detoxification tissue culture seedling with the sponge as the support, because liquid culture is adopted, the use of agar is saved, the cost is reduced by 60%, and the preparation flow of the culture medium is simplified; because the liquid culture medium has good fluidity and nutrients can be fully absorbed, the amount of the added culture solution can be saved by 30 percent, and the concentration of the culture solution can be reduced by 50 percent (compared with a solid culture medium).
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and such changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A liquid culture method of sweet potato detoxified tissue culture seedlings by taking sponge as a support is characterized by comprising the following steps:
s1, sponge processing: processing sponge into sponge blocks, and forming holes matched with the diameter of the stem sections of the sweet potato virus-free tissue culture seedlings in the middle of the sponge blocks;
s2, carrying out liquid micro-cuttage culture on the detoxified tissue culture seedlings of sweet potatoes: when the sweet potato detoxified tissue culture seedlings are subjected to micro cuttage, the stem sections of the sweet potato detoxified tissue culture seedlings are inserted into the sponge blocks, and liquid proliferation nutrient solution or rooting nutrient solution is poured into the sponge blocks for proliferation culture or rooting culture.
2. The liquid culture method for sweet potato detoxified tissue culture seedlings with sponges as supports of claim 1, wherein in the step S2, micro-cuttage propagation culture is performed, when sweet potato tissue culture seedlings grow to 8-12cm high and 5-6 leaves, the sweet potato tissue culture seedlings are cut according to 1-2 leaves/stem segment, the stem segment is 1.5-2cm long, and each segment is inserted into the hole of 1 new sponge square for propagation culture; and adding new liquid proliferation nutrient solution into the culture bottle of the old seedling, and growing and propagating the old seedling again after obtaining the new nutrient solution.
3. The liquid culture method for the detoxified tissue culture seedlings of sweet potatoes with sponges as supports according to claim 2, wherein the micro-cuttage propagation culture in the step S2 comprises the following steps:
s2-1: the liquid proliferation nutrient solution comprises 1/2MS, 6-BA0.1mg/L and 3% of cane sugar, the pH value is 5.8, and agar powder is not added; adding 30mL of liquid proliferation nutrient solution into a culture bottle, placing 6-8 sponge squares punched in the step S1 into the culture bottle, and inoculating seedlings after high-temperature sterilization;
s2-2: inoculating, cutting the stem section of the sweet potato virus-free tissue culture seedling into 1-3cm long, and inserting the stem section with 1-2 leaves into the sponge square hole;
s2-3: culturing at 24-26 deg.C under illumination of 2000lx for 14h/d; in the culture period of 30 days, when the plant grows to be 8-12cm high and more than 5-6 leaves, the micro-cuttage culture can be repeatedly carried out;
s2-4: micro-cuttage, namely repeating the step S2-2, cutting the plants into stem segments with the length of more than 2cm and 1-2 leaves, re-inserting the stem segments into the sponge square holes, and putting the stem segments into a culture bottle of liquid proliferation nutrient solution for proliferation culture;
s2-5: and (4) performing proliferation culture on the old seedlings, and adding a new liquid proliferation nutrient solution into a culture bottle of the original mother seedlings to grow again for proliferation culture.
4. The liquid culture method of detoxified tissue culture seedlings of sweet potatoes by using sponges as supports according to claim 1, characterized in that in step S2, rooting culture of detoxified tissue culture micro-cutting seedlings of sweet potatoes is carried out, the culture bottle is changed into liquid rooting nutrient solution, namely, the rooting culture is changed, after the detoxified tissue culture seedlings take roots, the rooting seedlings are taken out together with sponge blocks and directly planted into a substrate, after 1 week of heat preservation, moisture preservation and light-resistant domestication culture, the tissue culture seedlings grow new roots in the substrate and then survive, after 2-3 weeks of growth, the detoxified tissue culture domesticated seedlings of sweet potatoes can be sold as original seeds.
5. The liquid culture method for the detoxified tissue culture seedlings of sweet potatoes with sponges as supports of claim 4, wherein the step S2 of rooting and nutrition of tissue culture micro-cutting seedlings comprises the following steps:
s2-1: the liquid rooting nutrient solution comprises 1/2MS and 3% of sucrose, the pH value is 5.8, and agar powder is not added; adding 30mL of liquid rooting nutrient solution into a culture bottle, placing 8-10 sponge blocks punched in the step S1 into the culture bottle, and inoculating seedlings after high-temperature sterilization;
s2-2: inoculating, namely inserting cut stem sections of the sweet potato virus-free tissue culture seedlings into sponge square holes, wherein 8-10 plants can be inserted into each bottle;
s2-3: culturing at 26-28 deg.C under 3000lx for 12h/d; culturing for 20-25 days, and acclimating when the root system grows out of the sponge square;
s2-4: and (3) performing rooting culture on the old seedlings, and after the propagation and micro-cuttage culture of the mother seedlings, replacing the mother seedlings in a culture bottle with liquid rooting nutrient solution to perform rooting culture, wherein the old seedlings are used for rooting, so that the number of the rooted seedlings is increased.
6. The liquid culture method for the detoxified tissue culture seedlings of sweet potatoes by using a sponge as a support according to claim 5, wherein the step S2-3 is that the domestication of the rooted seedlings to the seedlings specifically comprises the following steps:
(1) Soaking a 50-hole plug tray in 0.1% potassium permanganate solution for 30 minutes for later use;
(2) Preparing a domestication substrate: mixing turf and perlite according to a volume ratio of 3;
(3) Rinsing the rooted tissue culture seedling in 500 times diluted carbendazim solution once, and then planting the sponge square with the tissue culture seedling into a matrix;
(4) Building a small arched shed, and covering a film and a sunshade net;
(5) Controlling the temperature at 20-30 deg.C, and gradually ventilating and exposing; after one week, the film can be lifted for normal management.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706880A (en) * | 2016-03-15 | 2016-06-29 | 宁夏农林科学院 | Purple sweet potato virus-free seedling water culture fast breeding method |
| CN108935108A (en) * | 2018-09-29 | 2018-12-07 | 河南云帮农业科技有限公司 | A kind of sweet potato tissue-cultured seedling detoxification tissue culture method |
| CN109197590A (en) * | 2018-10-24 | 2019-01-15 | 河南云帮农业科技有限公司 | A kind of sweet potato detoxication and tissue culture strong sprout method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105706880A (en) * | 2016-03-15 | 2016-06-29 | 宁夏农林科学院 | Purple sweet potato virus-free seedling water culture fast breeding method |
| CN108935108A (en) * | 2018-09-29 | 2018-12-07 | 河南云帮农业科技有限公司 | A kind of sweet potato tissue-cultured seedling detoxification tissue culture method |
| CN109197590A (en) * | 2018-10-24 | 2019-01-15 | 河南云帮农业科技有限公司 | A kind of sweet potato detoxication and tissue culture strong sprout method |
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