CN102210266A - Culture medium for culturing lilium pumilum tissues - Google Patents
Culture medium for culturing lilium pumilum tissues Download PDFInfo
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- 241001634090 Lilium pumilum Species 0.000 title claims abstract description 12
- 239000001963 growth medium Substances 0.000 title claims abstract description 11
- 238000012258 culturing Methods 0.000 title abstract 3
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 23
- 230000035755 proliferation Effects 0.000 claims abstract description 5
- 239000002609 medium Substances 0.000 claims description 46
- 241001359444 Lilium tenuifolium Species 0.000 claims description 39
- 230000001939 inductive effect Effects 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 239000006870 ms-medium Substances 0.000 claims 2
- 239000005556 hormone Substances 0.000 abstract description 9
- 229940088597 hormone Drugs 0.000 abstract description 9
- 230000012010 growth Effects 0.000 abstract description 7
- 238000011161 development Methods 0.000 abstract description 3
- 230000006698 induction Effects 0.000 abstract description 2
- 241000131317 Capitulum Species 0.000 abstract 1
- 235000021048 nutrient requirements Nutrition 0.000 abstract 1
- 239000012883 rooting culture medium Substances 0.000 abstract 1
- 241000234435 Lilium Species 0.000 description 12
- 238000009395 breeding Methods 0.000 description 12
- 230000001488 breeding effect Effects 0.000 description 12
- 238000012360 testing method Methods 0.000 description 9
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 8
- 230000004069 differentiation Effects 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 244000223014 Syzygium aromaticum Species 0.000 description 7
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 7
- 238000011081 inoculation Methods 0.000 description 6
- 241001656752 Lilium philadelphicum Species 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 230000003716 rejuvenation Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000001784 detoxification Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000206575 Chondrus crispus Species 0.000 description 1
- 244000061408 Eugenia caryophyllata Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 241001315001 Lilium concolor Species 0.000 description 1
- 244000210789 Lilium lancifolium Species 0.000 description 1
- OTCCIMWXFLJLIA-BYPYZUCNSA-N N-acetyl-L-aspartic acid Chemical compound CC(=O)N[C@H](C(O)=O)CC(O)=O OTCCIMWXFLJLIA-BYPYZUCNSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000034373 developmental growth involved in morphogenesis Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000012879 subculture medium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a culture medium for culturing lilium pumilum tissues. The best culture mediums for culturing the lilium pumilum tissues at different stages are screened; the proportion of various hormone in the culture medium is determined; and the culture mediums prepared according to the formula can meet the nutrient requirement and growth and development of the lilium pumilum at each stage. The culture mediums comprise a first generation induction culture mediums consisting of MS, 1.0 mg/L of 6-BA and 0.1 mg/L of NAA, a subgeneration proliferation culture medium consisting of MS, 0.2 mg/L of 6-BA and 0.01 mg/L of NAA, a rooting culture medium consisting of 1/2 MS and 0.5 mg/L of IBA and a capitulum culture medium consisting of 1/2 MS and 0.5 mg/L of IBA and 2 percent of sugar.
Description
Technical field
The invention belongs to plant biotechnology field, specifically, relate to the medium in each stage in the tissue culture of a kind of Lilium tenuifolium (Lilium pumilum).The present invention filters out the optimal medium that is used for Lilium tenuifolium tissue culture different times, has determined the proportioning of each hormone in the medium, can satisfy the nutritional need in each period of Lilium tenuifolium and grows according to the medium of formulated of the present invention.
Background technology
Lilium tenuifolium (Lilium pumilum) has another name called morningstar lily flower, is the perennial bulb class of Liliaceae lilium flowers.Lilium tenuifolium is grown in arid, semiarid zone, indomitable vitality is arranged, can survive the winter smoothly in the open air, also can normal growth under few at rainwater, as not have pouring situation, performance and attractive in appearance on can both reach the requirement of greening, can afforest in drought resisting and be used in the view and promote.But owing to from the threat of the mankind or non-human two aspects, make its population fail to breed in a large number under field conditions (factors), quantity is few and exist the nature degenerate problem.The way that solves is exactly the tissue culture that starts Lilium tenuifolium, and this is the effective ways of breeding fast and detoxification rejuvenation.
The tissue culture of lily starts from the 1950's, from nineteen fifty-seven Robb carries out the tissue culture achieving success first with lily bud scale after, lily in-vitro propagate technology is increasingly mature, and the development of aspects such as the quick breeding of improved seeds, rearing new variety and germ plasm resource preservation is very fast.Up to now, the lily kind of tissue culture success both at home and abroad and kind have surpassed hundreds of, but what be more common in report is to be used for the cultivar that fresh cut-flowers is produced, and be less to the Study on tissue culture of the wild lily of China.Sum up the data in literature over nearly 20 years, the only wild lily tissue culture success of kind surplus in the of ten is about caning be counted on one's fingers especially of Lilium tenuifolium.Under laboratory condition, the growth of control Lilium tenuifolium and breeding are one needs practice to go the new problem of groping and inquiring into.Prior art can reference data few, all must settle one by one by test, key will solve explant and draw materials position, period; The selection of each cultivation period medium; Condition of culture and Cheng Miaohou fast breeding technique etc.Wild resource with China's abundant carries out the kind innovation, use diversified breeding technique, on the basis of paying attention to the conventional hybridization breeding, carry out breeding of new variety in conjunction with the modern biotechnology means, carrying out the batch production exploitation of kind of ball with the detoxication and tissue culture seedling as the yielding ability original seed simultaneously, is the developing direction of lily tissue culture.The artificial propagation of Lilium tenuifolium is still mainly adopted to divide and is planted clove, seeding method and scale cuttage, breeds limited amount and very easily causes disease to infect and quality deterioration.But can be used for separating the degeneration problem at present and upgrade the tissue culture method breeding research of rejuvenation less, therefore carrying out the Lilium tenuifolium Study on tissue culture in a deep going way has become researcher and has demanded one of work of carrying out urgently.
The tissue culture method breeding is drawn materials conveniently; be not subjected to the restriction of natural conditions; can constantly breed in a large number; it is the most effectual way of Lilium tenuifolium introducing and planting, quick breeding, detoxification rejuvenation and rearing new variety; can obtain a large amount of seedlings in a short time; solved the less problem of provenance in the cultivation, for Lilium tenuifolium research and scale, industrialization development utilization from now on provides theoretical foundation.Domestic and international many scholars also study the tissue culture of lily at present, be applied at present in the lily production, but what be more common in report is that cultivar is used for fresh cut-flowers production, medium and method also are not suitable for Lilium tenuifolium, the present invention has systematically studied the optimal medium of different phase in the training of Lilium tenuifolium group, and this medium is also cultivated lily applicable to wild tiger lily lily and part.
Summary of the invention
The complete procedure of tissue culture generally is divided into several sport technique segments such as domestication transplanting of formulating culture scheme, explant selection and processing, inoculation, initial culture, successive transfer culture, strong sprout and culture of rootage, test-tube plantlet.Tissue culture is under isolated culture condition, and the histocyte of different plants and kindred plant different parts has only and satisfied their specific (special) requirements separately the nutritional requirement difference, could grow better.And grasp culture medium preparation and screening technique are to obtain one of successful key link of group training.
The objective of the invention is to filter out the optimal medium that is used for Lilium tenuifolium tissue culture different times, determined the proportioning of each hormone in the medium, can satisfy the nutritional need in each period of Lilium tenuifolium and grow according to the medium of formulated of the present invention.
The present invention has formulated embodiment on the basis of having carried out information search, gather and having analyzed, comprehensive, multi-faceted organize the training experiment, and the present invention has screened from first generation and induced → medium of subculture increment → culture of rootage → different times such as balling cultivation.Make first generation, breed, take root and the cultivation in balling each period reaches optimum efficiency.
Embodiment of the present invention comprise the optimal medium of each breeding time in the whole tissue culture procedures of Lilium tenuifolium, they draw through careful, repeated tests, are that the needs according to Lilium tenuifolium each breeding time have added dissimilar plant growth substances and different proportionings on the basis of minimal medium.
In embodiments of the invention, a kind of Lilium tenuifolium (Lilium pumilum) required medium of each stage of tissue culture procedures is provided, it is characterized in that this medium is by just forming for inducing culture, proliferated culture medium, root media, balling medium.These Lilium tenuifolium groups are trained each stage optimal medium and are filtered out through comparative test.
One embodiment of the present invention has provided the first for inducing culture of a kind of Lilium tenuifolium (Lilium pumilum): MS+6-BA 1mg/L+NAA 0.1mg/L+Vc 5mg/L.By discovering, the medium of no hormone is very little to the inducing action of clove, and hormone commonly used has NAA, 6-BA etc.Wherein NAA and IAA help the formation of root; And 6-BA is indispensable composition for inducing of bud, but during the 6-BA excessive concentration, though the clove bulbous protrusion that differentiates is many, but it is very thin and delicate, final is vitrified invalid seedling, NAA/6-BA influences the differentiation degree of root in the initial culture or bud, is MS+6-BA 1.0mg/L+NAA 0.1mg/L+Vc 5mg/L for the prescription of inducing culture just thereby filter out best.
One embodiment of the present invention has provided the proliferated culture medium of a kind of Lilium tenuifolium (Lilium pumilum): MS+6-BA 0.2mg/L+NAA 0.01mg/L.By discovering, the 6-BA of same high concentration has inhibitory action to lily bulb propagation, and excessive concentration can suppress the growth of bud, along with increasing of 6-BA concentration, when 6-BA concentration surpasses 2.0mg/L, grows thickly and downgrades obviously, easily produces the vitrifying seedling.The optimum multiplication medium prescription that filters out is MS+6-BA 0.2mg/L+NAA0.01mg/L.
One embodiment of the present invention has provided a kind of Lilium tenuifolium (Lilium pumilum) root media: 1/2MS+IBA 0.5mg/L.From 20 days result of the test of culture of rootage, low salt concn is favourable to the growth of wild lily root, and the prescription of suitable medium of taking root is 1/2MS+IBA 0.5mg/L.
One embodiment of the present invention has provided a kind of Lilium tenuifolium (Lilium pumilum) balling medium: 1/2MS+IBA 0.5mg/L+ white sugar 2%.By discovering, no matter Lilium tenuifolium is at MS or in the 1/2MS medium, the mass/volume degree of edible white sugar increases, balling all there is facilitation, it is even more ideal to compare 1/2MS+IBA 0.5mg/L+ white sugar 2%, balling quantity is many, and bulb is bigger, transplants the emergence rate best results.
In embodiment of the present invention, in scope as well known to those skilled in the art, NAA, 6-BA, IBA, V
cBe respectively methyl, 6-benzyl aminopurine, indolebutyric acid, ascorbic abbreviation.
In the whole tissue culture procedures of the Lilium tenuifolium that the present invention selects the optimal medium of each breeding time can fully satisfy each period of Lilium tenuifolium nutritional need and grow; be to guarantee the successful implementation of Lilium tenuifolium tissue culture; reach key and core that procedure is produced, for the foundation and the large-scale production of Lilium tenuifolium regenerating system provides strong support.
Embodiment
The material of embodiment 1. Lilium tenuifoliums and screening statistical method
The Lilium tenuifolium of experiment usefulness is in picking up from Daqunshan Mountains, suburb nearby, Huhehaote City, and the bulb that collects is planted test nursery in our company, replenishes according to test progress, different times sampling and sampling at any time.Explant is the bulb of Lilium tenuifolium.
With MS is minimal medium, makes curing agent with the carragheen of 3.5g/L, and 3% edible white sugar place of sucrose replaces distilled water as carbon source with running water, and the basic element of cell division and the growth hormone that add different proportionings respectively carry out the screening of medium.
The screening that embodiment 2. Lilium tenuifolium groups are trained each stage optimal medium
1, the selection of inductive differentiation medium
The concentration of 6-BA is established 0.2mg/L, 0.5mg/L, five concentration gradients of 1.0mg/L, 2.0mg/L, the concentration of NAA is established 0.1mg/L, 0.2mg/L, four concentration gradients of 0.3mg/L, 0.5mg/L, carry out different assembly, every bottle graft a slice bulb during inoculation, observe scale on different medium indefinite bud a situation arises.
As seen from Table 1,7 kinds of hormone combinations all can be induced lily bud scale differentiation clove, but the differentiation rate difference of each combination, No. 1 MS+6-BA 0.1mg/L+NAA 0.1mg/L is that Lilium tenuifolium scale medium is best inducing culture, inductivity height not only, and the newly-increased bud number of each explant is many, and differentiation rate is the highest, and its differentiation rate can reach 60.0%.
Concrete statistical formula is:
The explant sum of survival rate=viable explant number/inoculation) * 100%
Just for bud induction rate=(the explant sum of the explant number/inoculation of sprouting) * 100%
Shoot proliferation multiple=(sum of the bud number of the growing thickly/access individual plant bud that induces) * 100%
Rooting rate=(the explant sum of the explant number/inoculation of differentiation root) * 100%
Clove inductivity=(the explant sum of explant number/inoculation of differentiation clove) * 100%
Table 1 hormone concentration and combination thereof are to the influence of Lilium tenuifolium scale adventitious bud inducing
2, the selection of shoot proliferation medium
To induce the indefinite bud that differentiates to be cut into individual plant, be inoculated on the proliferated culture medium, 6-BA establishes 0.2mg/L, 0.5mg/L, four concentration gradients of 1.0mg/L, 2.0mg/L, NAA establishes 0.01mg/L, 0.1mg/L, four concentration gradients of 0.2mg/L, 0.3mg/L totally seven kinds of medium, 4 individual plants of every bottle graft, the differential growth situation of observed and recorded regenerated adventitious bud, the rate of increase of bud under the statistics different hormone combinations condition after 30 days.
Behind inducing culture, the 2.0-4.0cm budlet that will produce in inducing culture moves into (referring to table 2) in the proliferated culture medium respectively, and the bastem portion of 15 days rear section materials begins observed and recorded, statistical results after 30 days when the bud clump occurring.Observation shows that the 6-BA of high concentration has inhibitory action to lily bulb propagation, and excessive concentration can suppress the growth of bud, along with increasing of 6-BA concentration, when 6-BA concentration surpasses 2.0mg/L, grows thickly and downgrades obviously, easily produces the vitrifying seedling.The optimum multiplication medium prescription that filters out is MS+6-BA0.2mg/L+NAA0.01mg/L.
Table 2 hormone concentration and combination thereof are to the influence of Lilium tenuifolium adventitious bud proliferation
3, the selection of root media
With the seedling preferably of growing way in the subculture medium, be divided into individual plant and change in the root media, every bottle graft is gone into 4 strains, and the situation of taking root of seedling was added up rooting rate after 20 days on the different medium of observed and recorded.
From 20 days result of the test of culture of rootage, two medium all have inducing action to the formation of root, and wherein No. 2 root media rooting efficiencies are better, and rooting rate reaches 88.89%.The base portion of taking root can increase the clove of band root newly, on average can reach 3-4.As seen low salt concn is favourable to the growth of wild lily root, and suitable medium of taking root is 1/2MS+IBA0.5mg/L (table 3).
The influence that table 3 mineral salt are taken root to seedling
4, the take root selection of balling medium
Based on the basis of root media, the bud of growing thickly of propagation is inoculated into edible white sugar content brings up on the medium of 40-50g/L, observe the situation of inducing of test tube clove, did once statistics in 30 days.
Our test shows no matter Lilium tenuifolium is to cultivate at MS or at 1/2MS to concentrate, and sugared content increases to 2%, balling is all had facilitation, and it is even more ideal to compare 1/2MS+IBA 0.5mg/L+ white sugar 2%, and balling quantity is many, bulb is bigger, transplants the emergence rate best results.
Above embodiment purpose is for specifying content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, modification or replacement to the inventive method, step or condition are done all belong to scope of the present invention.
Claims (5)
1. Lilium tenuifolium (Lilium pumilum) required medium of each stage of tissue culture procedures is characterized in that, this medium by just for inducing culture, the shoot proliferation medium, root media, the balling medium is formed.
2. first for inducing culture described in the claim 1, it consists of and contains 1.0mg/L 6-BA, 0.1mg/L NAA, the MS medium of 5mg/LVc.
3. the proliferated culture medium described in the claim 1, it consists of and contains 0.2mg/L 6-BA, the MS medium of 0.01mg/LNAA.
4. the root media described in the claim 1, it consists of the 1/2MS medium that contains 0.5mg/L IBA.
5. the balling medium described in the claim 1, it consists of and contains 0.5mg/L IBA, the 1/2MS medium of additional 2% white sugar.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222427A (en) * | 2013-05-08 | 2013-07-31 | 四川农业大学 | Method for efficiently inducing Luding lily test tube bulbels |
| CN104604676A (en) * | 2014-12-30 | 2015-05-13 | 内蒙古和信园蒙草抗旱绿化股份有限公司 | Culture media for tissue culture of red lily |
| CN113317206A (en) * | 2021-07-19 | 2021-08-31 | 中国科学院昆明植物研究所 | Culture medium combination for tissue culture and rapid propagation of lilium martagon, tissue culture and rapid propagation method and application |
-
2011
- 2011-03-24 CN CN 201110071332 patent/CN102210266B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 《东北林业大学学报》 20100531 葛蓓孛，等 细叶百合组织培养植株再生 第38卷, 第5期 * |
| 《安徽农业大学学报》 20010428 傅玉兰，等 影响百合试管鳞茎增殖因素的研究 第28卷, 第2期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222427A (en) * | 2013-05-08 | 2013-07-31 | 四川农业大学 | Method for efficiently inducing Luding lily test tube bulbels |
| CN104604676A (en) * | 2014-12-30 | 2015-05-13 | 内蒙古和信园蒙草抗旱绿化股份有限公司 | Culture media for tissue culture of red lily |
| CN113317206A (en) * | 2021-07-19 | 2021-08-31 | 中国科学院昆明植物研究所 | Culture medium combination for tissue culture and rapid propagation of lilium martagon, tissue culture and rapid propagation method and application |
| CN113317206B (en) * | 2021-07-19 | 2021-11-05 | 中国科学院昆明植物研究所 | Culture medium combination for tissue culture and rapid propagation of lilium martagon, tissue culture and rapid propagation method and application |
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Address after: 011517 the Inner Mongolia Autonomous Region Hohhot city Shengle Economic Zone Shengle five Street South Patentee after: INNER MONGOLIA MONGOLIAN GRASS ECOLOGICAL ENVIRONMENT (GROUP) Ltd. Address before: 010030 University of the Inner Mongolia Autonomous Region Hohhot City No. 71 West Yindu building B block 3 layer Patentee before: INNER MONGOLIA HEXINYUAN MONSOD DROUGHT-RESISTANCE GREENING Co.,Ltd. |
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Effective date of registration: 20180806 Address after: 011517 the Inner Mongolia Autonomous Region, Hohhot, Helingeer County Sheng Le economic park five street south of Sheng le Co-patentee after: INNER MONGOLIA MENGCAO GRASS AND SEED INDUSTRY Co.,Ltd. Patentee after: INNER MONGOLIA MONGOLIAN GRASS ECOLOGICAL ENVIRONMENT (GROUP) Ltd. Address before: 011517 Sheng Le economic Park, Hohhot, the Inner Mongolia Autonomous Region, south of five street, Sheng le. Patentee before: INNER MONGOLIA MONGOLIAN GRASS ECOLOGICAL ENVIRONMENT (GROUP) Ltd. |
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Denomination of invention: Culture medium for culturing lilium pumilum tissues Effective date of registration: 20200107 Granted publication date: 20130417 Pledgee: Inner Mongolia Hohhot Jingu Rural Commercial Bank Co.,Ltd. Zhaowuda sub branch Pledgor: INNER MONGOLIA MONGOLIAN GRASS ECOLOGICAL ENVIRONMENT (GROUP) Ltd. Registration number: Y2020150000002 |
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Address after: 011517 the Inner Mongolia Autonomous Region, Hohhot, Helingeer County Sheng Le economic park five street south of Sheng le Patentee after: Mengcao ecological environment (Group) Co.,Ltd. Country or region after: China Patentee after: Inner Mongolia Mengcao Grass Industry Technology Co.,Ltd. Address before: 011517 the Inner Mongolia Autonomous Region, Hohhot, Helingeer County Sheng Le economic park five street south of Sheng le Patentee before: INNER MONGOLIA MONGOLIAN GRASS ECOLOGICAL ENVIRONMENT (GROUP) Ltd. Country or region before: China Patentee before: INNER MONGOLIA MENGCAO GRASS AND SEED INDUSTRY Co.,Ltd. |
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