CN101238795A - Artificial breeding method for spathiphyllum floribundum - Google Patents
Artificial breeding method for spathiphyllum floribundum Download PDFInfo
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- CN101238795A CN101238795A CNA2008100707337A CN200810070733A CN101238795A CN 101238795 A CN101238795 A CN 101238795A CN A2008100707337 A CNA2008100707337 A CN A2008100707337A CN 200810070733 A CN200810070733 A CN 200810070733A CN 101238795 A CN101238795 A CN 101238795A
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Abstract
The invention provides an artificial propagation method of Spathiphylium Floribundum, which not only can maintian high propagation efficiency but also can keep high consistence and feature stability of plants. It includes five steps such as female parent pretreatment, explants selection, original variety production, original variety domestication, multiplication, rooting and transplantation. Rhizome and lateral bud are used as explants, and after disinfection they are inoculated onto a certain medium for inducing callus and adventitious buds, then a complete plant is formed, after induction and multiplication, the breeding speed is accelerated, the evenness is high, a large amount of obtained ramets characteristics of the clonal progeny of original good single plant or block mass are identical.
Description
Technical field
The present invention relates to a kind of plant artificial propagation techniques field, be specifically related to a kind of Spathiphyllum kochii artificial breeding method.
Background technology
Spathiphyllum kochii has another name called the white palm, is Araeceae Spathiphyllum kochii platymiscium, originates in the torrid areas in South America, and Spathiphyllum kochii leaf look delicate and pretty, and spathe is pure white, is that important sight flower is seen the leaf pot plant, also is suitable for view simultaneously and arranges.Later 1980s, China just began to introduce a fine variety, and developed in south rapidly.The production development of Spathiphyllum kochii and breeding of new variety are in the Europe starting early, Men Fanwen company, Belgian moral Meyer--De Luke company as Holland, Milestone (Milestone) agricultural corporation of the U.S. etc. is arranged in recent years, all is to come supply the market with industrialization production Spathiphyllum kochii, release new varieties.At present, the breed breeding of Spathiphyllum kochii all is by traditional hybridization mode, adopts different parent's hybridization of introducing a fine variety ground, different cultivars, obtain hybrid seed,, filter out the fine individual plant that is fit to market prospects by the matrix sowing, set up clone through tissue culture technique, thereby form new kind.The propagation method commonly used of known Spathiphyllum kochii has three kinds: the one, and division propagation, its shortcoming is that reproduction speed is slow; The 2nd, seed propagation, but ripening rate is low under field conditions (factors) because of Spathiphyllum kochii, and percentage of seedgermination is low, and poor through the taro seedling regularity of planting seed.
Summary of the invention
The purpose of this invention is to provide a kind of Spathiphyllum kochii artificial breeding method who can breed fast and can keep the height uniformity and the stability of plant forms feature.
Technical scheme that the present invention adopts is such: a kind of Spathiphyllum kochii artificial breeding method comprises the steps:
1) maternal preliminary treatment: the whole strain from basin of Spathiphyllum kochii maternal plant is taken out, remove root system, clean up the acquisition rhizome after divesting blade;
2) explant collection and original seed are made: the rhizome exocuticle root eye on rhizome of pruning is eliminated clean, remove the rhizome top vane and keep growing point, the length of whole rhizome is got 10～20mm, uses rinsed with sterile water 3～5 times, each 3～5 minutes after rhizome is carried out disinfection;
3) original seed is induced: the original seed that step 2 is made is inoculated in the 6-BA that the MS improved culture medium is added 3～5mg/1,0.5 the NAA of～1mg/1, the sucrose of 15～40g/1 and the agar of 6～8g/1, and at 26 ± 3 ℃, cultivate under the condition of intensity of illumination 300～5001x, illumination every day 12～14 hours, cultivate after 4～5 weeks when terminal bud extraction length during greater than 20mm, remove that top vane is left and taken 8～10mm base portion and to cuing open growing point, cut into if basal growth goes out a plurality of budlets 1～3 bud/, original seed after dissecing or group are inoculated in the 6-BA that the MS improved culture medium is added 3～5mg/1,0.5 the NAA of～1mg/1, the sucrose of 15～40g/1 and the agar of 6～8g/1, and at 26 ± 3 ℃, condition following every day of the illumination of intensity of illumination 1500～20001x 12～14 hours is cultivated and 4～5 weeks was obtained to grow thickly sorite;
4) propagation expands numerous: the sorite that grows thickly that will produce cuts into 3～5 buds/agglomerate, when bud on the agglomerate tall and big during in 25mm, from removing top from the 20mm of bastem portion, then each agglomerate is inoculated in the sucrose of NAA, 15～40g/1 of 6-BA, 0.5～1mg/1 of the additional 3～5mg/1 of MS improved culture medium and the agar of 6～8g/1, and, cultivate and 4～6 weeks obtained more to grow thickly sorite 26 ± 3 ℃, condition following every day of the illumination of intensity of illumination 1500～20001x 12～14 hours;
5) strengthening seedling and rooting: the sorite that grows thickly that step 4 is made be divided into 2～4 buds/, be inoculated in the MS improvement strong seedling culture base of agar of the sucrose of active carbon, 15～40g/1 of additional 0.5～1mg/1 and 6～8g/1, and 26 ± 3 ℃, condition following every day of the illumination of intensity of illumination 1500～25001x 12～14 hours, cultivate the seedling group that obtains to have 5～10 buds 6～8 weeks, high 15～the 30mm of bud, seedling group is cut into the individual plant or the agglomerate of 1～3 bud, can obtain the strain of finished product Spathiphyllum kochii tissue cultivating seedling.
Above-mentioned Spathiphyllum kochii artificial breeding method, the disinfectant that adopts in the described step 2 is that concentration is 0.1% mercuric chloride solution.
Above-mentioned Spathiphyllum kochii artificial breeding method, maternal plant 7-10 days before from basin, taking out in the described step 1, the fertilising of not watering is slightly wilted the blade of maternal plant.
By adopting aforementioned artificial breeding method, the invention has the beneficial effects as follows that the stem apex by adopting rhizome and lateral bud is an explant, after sterilization, be inoculated into and induce callus and indefinite bud on the certain culture base, and form complete plant, by induce, breed expand numerous and strengthening seedling and rooting after, it is bred speed and accelerates greatly, plant regularity height, and a large amount of former choiceness seedling that is obtained is consistent with the maternal character performance.
Embodiment
The notification number of on November 1st, 2006 bulletin is that the disclosed step of breeding of the disclosed oncidiumLuridum high-quality of CN1282409C seed method for quickly breeding comprises: draw materials, bud is grown thickly in the sterilization of explant, bennet induces, stem apex is grown thickly bud induces, from seven steps such as the propagation of sprouting, strong plantlets and rootage cultivation, test-tube seedling transplantings, read over disclosed correlation step of this specification and the condition of culture that is adopted, nutrient solution etc. and help to understand better the whole step of breeding of the present invention, enumerate a specific embodiment below also to describe in detail:
A kind of Spathiphyllum kochii (being named as " dream of sunlight ") artificial breeding method may further comprise the steps:
1) maternal preliminary treatment: Spathiphyllum kochii maternal plant to be bred fertilising 7～10 days that stop to water, the maternal plant blade is slightly wilted, maternal plant whole strain from basin is taken out, slice off its root system, divest blade, clean up the rhizome of acquisition with running water with sharp sword.
2) explant collection and original seed are made: the rhizome exocuticle of pruning, the root eye eliminates totally on rhizome, remove top vane and keep growing point, the length of controlling whole rhizome between 10～20mm as 15mm, carrying out the sterilization of branch bottle according to the rhizome diameter, promptly is 0.1% mercuric chloride (HgC1 with concentration
2) carried out disinfection 25～35 minutes, use rinsed with sterile water again 3～5 times, each 3～5 minutes, the rhizome that rinsing is good cuts the cross section variable color partly can make original seed.
3) original seed is induced: the original seed that step 2 is made is inoculated in the 6-BA that the MS improved culture medium is added 3～5mg/1,0.5 the NAA of～1mg/1, the sucrose of 30g/1 and the agar of 6～8g/1, and at 26 ± 1 ℃, cultivate under the condition of intensity of illumination 300～5001x, illumination every day 12～14 hours, cultivate after 4～5 weeks when terminal bud extraction length during greater than 20mm, remove that top vane is left and taken 8～10mm base portion and to cuing open growing point (being split into two halves) but it can not be dissectd, cut into if basal growth goes out a plurality of budlets 1～3 bud/, original seed after dissecing or group are inoculated in the 6-BA that the MS improved culture medium is added 3～5mg/1,0.5 the NAA of～1mg/1, the sucrose of 30g/1 and the agar of 6～8g/1, and at 26 ± 1 ℃, condition following every day of the illumination of intensity of illumination 1500～20001x 12～14 hours, cultivate and 4～5 weeks obtained to grow thickly sorite, each sorite that grows thickly has 10～15 buds of growing thickly, and the height of the bud of growing thickly is greater than 25mm.
4) propagation expands numerous: the sorite that grows thickly that will produce cuts into 3～5 buds/agglomerate, when bud on the agglomerate tall and big during in 25mm, from removing top from the 20mm of bastem portion, then each agglomerate is inoculated in the sucrose of NAA, 30g/1 of 6-BA, 0.5～1mg/1 of the additional 3～5mg/1 of MS improved culture medium and the agar of 6～8g/1, and, cultivate and 4～6 weeks obtained more to grow thickly sorite 26 ± 1 ℃, condition following every day of the illumination of intensity of illumination 1500～20001x 12～14 hours.
5) strengthening seedling and rooting: the sorite that grows thickly that step 4 is made be divided into 2～4 buds/, be inoculated in the MS improvement strong seedling culture base of agar of the sucrose of active carbon, 30g/1 of additional 0.5～1mg/1 and 6～8g/1, and 26 ± 1 ℃, condition following every day of the illumination of intensity of illumination 1500～25001x 12～14 hours, cultivate the seedling group that obtains to have 5～10 buds 6～8 weeks, high 15～the 30mm of bud, by 1～3 bud/, each is more than the main bud high 25mm of group and have 2 standards of launching leaf fully seedling group is cut, and can obtain the strain of finished product Spathiphyllum kochii seedling.
" dream of the sunlight " Spathiphyllum kochii that obtains by previous embodiment is to be female parent with Spathiphyllum ' Chris2# ' (Jesus 2#) Spathiphyllum kochii, Spathiphyllum ' Rhapsody ' Robertson Spathiphyllum kochii is made male parent, adopt the conventional hybridization method, the new varieties of cultivating through seed selection for many years.The strain shape of " dream of sunlight " Spathiphyllum kochii is middle-size and small-size, plant height is between two parents, leaf color ratio female parent is more dark green, and white spathe size is better than female parent, maternal plant petal length of a film 10.5-12.5cm, wide 6.0-7.5cm, the clone semi-finished product long 11.0cm of strain bract of blooming, wide 4.7cm, bennet is strong, uprightly, highly moderate.
In the foregoing description, described MS improved culture medium, 6-BA, NAA etc. belong to material known in plant artificial breeding field, and each material and reagent can be buied from disclosed market, and the above-mentioned CN1282409C of Chinese patent formerly also relates to corresponding material.And each additional composition of cultivating can change in span separately relatively, even certain span surpasses claim institute restricted portion, it can realize the object of the invention equally, and it is less better relatively just to breed time or effect.
Claims (3)
1, a kind of Spathiphyllum kochii artificial breeding method is characterized in that comprising the steps:
1) maternal preliminary treatment: the whole strain from basin of Spathiphyllum kochii maternal plant is taken out, remove root system, clean up the acquisition rhizome after divesting blade;
2) explant collection and original seed are made: the rhizome exocuticle root eye on rhizome of pruning is eliminated clean, remove the rhizome top vane and keep growing point, the length of whole rhizome is got 10～20mm, uses rinsed with sterile water 3～5 times, each 3～5 minutes after rhizome is carried out disinfection;
3) original seed is induced: the original seed that step 2 is made is inoculated in the 6-BA that the MS improved culture medium is added 3～5mg/1,0.5 the NAA of～1mg/1, the sucrose of 15～40g/1 and the agar of 6～8g/1, and at 26 ± 3 ℃, cultivate under the condition of intensity of illumination 300～5001x, illumination every day 12～14 hours, cultivate after 4～5 weeks when terminal bud extraction length during greater than 20mm, remove that top vane is left and taken 8～10mm base portion and to cuing open growing point, cut into if basal growth goes out a plurality of budlets 1～3 bud/, original seed after dissecing or group are inoculated in the 6-BA that the MS improved culture medium is added 3～5mg/1,0.5 the NAA of～1mg/1, the sucrose of 15～40g/1 and the agar of 6～8g/1, and at 26 ± 3 ℃, condition following every day of the illumination of intensity of illumination 1500～20001x 12～14 hours is cultivated and 4～5 weeks was obtained to grow thickly sorite;
4) propagation expands numerous: the sorite that grows thickly that will produce cuts into 3～5 buds/agglomerate, when bud on the agglomerate tall and big during in 25mm, from removing top from the 20mm of bastem portion, then each agglomerate is inoculated in the sucrose of NAA, 15～40g/1 of 6-BA, 0.5～1mg/1 of the additional 3～5mg/1 of MS improved culture medium and the agar of 6～8g/1, and, cultivate and 4～6 weeks obtained more to grow thickly sorite 26 ± 3 ℃, condition following every day of the illumination of intensity of illumination 1500～20001x 12～14 hours;
5) strengthening seedling and rooting: the sorite that grows thickly that step 4 is made be divided into 2～4 buds/, be inoculated in the MS improvement strong seedling culture base of agar of the sucrose of active carbon, 15～40g/1 of additional 0.5～1mg/1 and 6～8g/1, and 26 ± 3 ℃, condition following every day of the illumination of intensity of illumination 1500～25001x 12～14 hours, cultivate the seedling group that obtains to have 5～10 buds 6～8 weeks, high 15～the 30mm of bud, seedling group is cut into the individual plant or the agglomerate of 1～3 bud, can obtain the strain of finished product Spathiphyllum kochii tissue cultivating seedling.
2, Spathiphyllum kochii artificial breeding method according to claim 1 is characterized in that: the disinfectant that adopts in the described step 2 is that concentration is 0.1% mercuric chloride solution.
3, Spathiphyllum kochii artificial breeding method according to claim 1 and 2 is characterized in that: maternal plant 7-10 days before taking out from basin in the described step 1, the fertilising of not watering is slightly wilted the blade of maternal plant.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326510A (en) * | 2011-08-23 | 2012-01-25 | 泉州市泉美生物科技有限公司 | Method for artificially breeding spathiphyllum |
| CN102388805A (en) * | 2011-09-09 | 2012-03-28 | 广东省农业科学院花卉研究所 | Spathiphyllum somatic embryo inducing and plant regenerating method |
| CN103749174A (en) * | 2013-12-31 | 2014-04-30 | 泉州市泉美生物科技有限公司 | Method for regulating flowering time of peace lily |
| CN104145820A (en) * | 2014-08-18 | 2014-11-19 | 安徽省农业科学院园艺研究所 | Tissue culture and rapid propagation method of taro with red buds |
| CN105028154A (en) * | 2015-06-29 | 2015-11-11 | 梁海林 | Soilless cultivation method for spathiphyllum |
| CN106106181A (en) * | 2016-08-08 | 2016-11-16 | 吴子平 | A kind of tissue culture and rapid propagation method of Spathiphyllum kochii |
| CN108834888A (en) * | 2018-05-25 | 2018-11-20 | 广东省农业科学院作物研究所 | A kind of proliferated culture medium and its proliferation strong seedling culture method of the white palm |
| CN109329055A (en) * | 2018-10-18 | 2019-02-15 | 广东海洋大学 | A kind of raising Spathiphyllum kochii adventitious bud proliferation rate reduces the culture medium of albefaction ratio again |
| CN109526748A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of Spathiphyllum kochii inflorescence method for tissue culture |
| CN109526747A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of white palm leaf tissue cultural method |
| CN109526746A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of Spathiphyllum kochii petiole method for tissue culture |
| CN110199872A (en) * | 2019-05-14 | 2019-09-06 | 广州花卉研究中心 | A kind of fast numerous method of the white palm Shoot-tip Culture of short disinfecting time |
| CN110199873A (en) * | 2019-05-14 | 2019-09-06 | 广州花卉研究中心 | A kind of fast numerous method of the white palm Shoot-tip Culture of low hormonal readiness |
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| CN102326510A (en) * | 2011-08-23 | 2012-01-25 | 泉州市泉美生物科技有限公司 | Method for artificially breeding spathiphyllum |
| CN102388805A (en) * | 2011-09-09 | 2012-03-28 | 广东省农业科学院花卉研究所 | Spathiphyllum somatic embryo inducing and plant regenerating method |
| CN102388805B (en) * | 2011-09-09 | 2013-05-01 | 广东省农业科学院花卉研究所 | Spathiphyllum somatic embryo inducing and plant regenerating method |
| CN103749174A (en) * | 2013-12-31 | 2014-04-30 | 泉州市泉美生物科技有限公司 | Method for regulating flowering time of peace lily |
| CN103749174B (en) * | 2013-12-31 | 2015-09-23 | 泉州市泉美生物科技有限公司 | Spathiphyllum kochii florescence control method |
| CN104145820A (en) * | 2014-08-18 | 2014-11-19 | 安徽省农业科学院园艺研究所 | Tissue culture and rapid propagation method of taro with red buds |
| CN105028154A (en) * | 2015-06-29 | 2015-11-11 | 梁海林 | Soilless cultivation method for spathiphyllum |
| CN105028154B (en) * | 2015-06-29 | 2017-09-22 | 梁海林 | A kind of white palm soilless culture method |
| CN106106181A (en) * | 2016-08-08 | 2016-11-16 | 吴子平 | A kind of tissue culture and rapid propagation method of Spathiphyllum kochii |
| CN108834888B (en) * | 2018-05-25 | 2022-03-25 | 广东省农业科学院作物研究所 | Proliferation culture medium of anthurium andraeanum and proliferation strong seedling culture method thereof |
| CN108834888A (en) * | 2018-05-25 | 2018-11-20 | 广东省农业科学院作物研究所 | A kind of proliferated culture medium and its proliferation strong seedling culture method of the white palm |
| CN109329055A (en) * | 2018-10-18 | 2019-02-15 | 广东海洋大学 | A kind of raising Spathiphyllum kochii adventitious bud proliferation rate reduces the culture medium of albefaction ratio again |
| CN109526747A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of white palm leaf tissue cultural method |
| CN109526746A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of Spathiphyllum kochii petiole method for tissue culture |
| CN109526748B (en) * | 2018-12-29 | 2022-02-18 | 贵州大学 | Tissue culture method for anthurium andraeanum inflorescence |
| CN109526748A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of Spathiphyllum kochii inflorescence method for tissue culture |
| CN109526746B (en) * | 2018-12-29 | 2022-07-05 | 贵州大学 | Tissue culture method for petioles of hairyvein agrimony |
| CN109526747B (en) * | 2018-12-29 | 2022-07-05 | 贵州大学 | Tissue culture method for spathiphyllum leaf |
| CN110199872A (en) * | 2019-05-14 | 2019-09-06 | 广州花卉研究中心 | A kind of fast numerous method of the white palm Shoot-tip Culture of short disinfecting time |
| CN110199873A (en) * | 2019-05-14 | 2019-09-06 | 广州花卉研究中心 | A kind of fast numerous method of the white palm Shoot-tip Culture of low hormonal readiness |
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