CN109197590A - A kind of sweet potato detoxication and tissue culture strong sprout method - Google Patents
A kind of sweet potato detoxication and tissue culture strong sprout method Download PDFInfo
- Publication number
- CN109197590A CN109197590A CN201811242428.1A CN201811242428A CN109197590A CN 109197590 A CN109197590 A CN 109197590A CN 201811242428 A CN201811242428 A CN 201811242428A CN 109197590 A CN109197590 A CN 109197590A
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- strong
- culture
- sweet potato
- seedling
- strong sprout
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The present invention relates to a kind of sweet potato detoxication and tissue culture strong sprout methods, specifically includes the following steps: managing after domestication, (6) transplanting before (1) detoxification treatment, (2) strong seedling culture, (3) increase illumination, (4) culture of rootage, (5) bottle outlet.The invention has the advantages that: a kind of sweet potato detoxication and tissue culture strong sprout method is provided, easy to operate, replicability is strong, promotes sweet potato bottle seedling stem thickness strong, and leaf is enlarged afforested area, and rooted seedling is healthy and strong, improves sweet potato bottle seedling quality, to improve the survival rate of sweet potato bottle seedling, improves Ipomoea batatas production.
Description
Technical field
The present invention relates to sweet potato planting technology field, in particular to a kind of sweet potato detoxication and tissue culture strong sprout methods.
Background technique
Sweet potato also known as sweet potato, Convolvulaceae Dioscorea wind herbaceous species, and underground stem tuber top branch ends enlarge into ovoid
Stem tuber, crust is faint yellow, smooth, and sweet potato belongs to the short day crop of light, and property happiness temperature can not resist cold, more drought-enduring, is mainly distributed on
On the south 40 ° of north latitude.The sweet potato bottle seedling speed of growth is fast, and breeding is fast, but sweet potato, with the increase of plantation algebra, virus infection causes
It will appear degradation phenomena.
Summary of the invention
The purpose of the present invention is to provide a kind of sweet potato detoxication and tissue culture strong sprout method, the kind of selection is Yunnan purple sweet potato, with
Solve the problems mentioned above in the background art.
In order to solve the above technical problems, technical solution provided by the invention are as follows: a kind of sweet potato detoxication and tissue culture strong sprout method, tool
Body the following steps are included:
(1) detoxification treatment: the healthy and strong disease-free sweet potato of choosing takes 1-5 centimetres of stem apex to sterilize on superclean bench as parent,
Stem apex removing is carried out with dissecting needle under anatomical lens after aseptic process, the shoot tip meristem of 0.2~0.4mm is cut, is seeded in 1/
On the solid medium of 2MS, NAA0.1mg/L, it is sent between culture and air-dries, it is desirable that 25-30 DEG C of temperature, daily 12h, 4000~
Cultivated under the illumination of 5000 luxs, generate plantlet, wait buds long to 3-5 centimetres of detection virus, transfer after detection is virus-free into
Enter fast numerous stage culture, detects confirmation without virus, so that it may which the MS for tapping into BA concentration 1mg/L, NAA concentration 0.1mg/L is numerous fastly
Culture medium progress is fast numerous, and bud is cut one by one when sprout length to 3 centimetres of sizes and is divided into list by the long budding clump of one month or so stem apex
Strain, cuts upper blade, is inoculated into the MS proliferated culture medium of BA concentration 1mg/L, NAA concentration 0.1mg/L and continues to be proliferated, and every bottle
Put 5 buds, every 15-25 days or so 1 generations of proliferation；
(2) strong seedling culture: culture medium gradually reduces hormone concentration, and proliferation seedling is inoculated into strong seedling culture when 6-8 generation breeds
It is cultivated in base；
(3) increase illumination: increasing illumination during strong sprout, illumination increases to 6000 luxs from 4000 luxs；
(4) culture of rootage: strong sprout, which is bred, is followed by root media 1/2MS, NAA0.1mg/L, in AC content 0.1%, chooses
Selecting sturdy young plant to be connected to root media is that it is promoted to take root, and weak young plant continues to be connected to strong sprout in strong seedling culture base；
(5) it tames: the sweet potato detoxic seedling after taking root being transplanted in greenhouse, hardening 15-20 days, temperature 18-28 before bottle outlet
Degree, illumination are no more than 8000 luxs, can cleaning and sterilizing plantation finishing scouring seedling 3~4 days after opening after 20 days；
(6) it is managed after transplanting: being uniformly mixed with vermiculite, perlite, the dregs of a decoction, the turf that ratio is 1:1:1:3, be layered on 1.2
On the wide seedbed of rice, 30 centimetres of seedbed interval is sprinkled profoundly water, then after being sterilized with fungicide, plants ipomoea batatas seedling, curing temperature 23-30 degree,
The lux humidity 60-90%, illumination 5000-8000, gradually increases after one month less than 10000 luxs.
As a preferred embodiment, the strong seedling culture base is 1/2MS culture medium, cycocel content 0.2mg/L, NAA
Content 0.05mg/L.
As a preferred embodiment, the root media is 1/2MS culture medium, NAA content 0.1mg/L, AC content
0.1%.
The invention has the advantages that: a kind of sweet potato detoxication and tissue culture strong sprout method is provided, easy to operate, replicability is strong, promotes
Sweet potato bottle seedling stem thickness is strong, and leaf is enlarged afforested area, and rooted seedling is healthy and strong, improves sweet potato bottle seedling quality, so that the survival rate of sweet potato bottle seedling is improved,
Improve Ipomoea batatas production.
Specific embodiment
Illustrate the present invention with specific embodiment below, is not limitation of the present invention.
Embodiment 1
A kind of sweet potato detoxication and tissue culture strong sprout method, specifically includes the following steps:
(1) detoxification treatment: the healthy and strong disease-free sweet potato of choosing takes 1 centimetre of stem apex to sterilize on superclean bench, nothing as parent
Stem apex removing is carried out with dissecting needle under anatomical lens after bacterium processing, the shoot tip meristem of 0.2mm is cut, is seeded in 1/2MS,
On the solid medium of NAA0.1mg/L, it is sent between culture and air-dries, it is desirable that 25-30 DEG C of temperature, daily 12h, 4000 luxs
It is cultivated under illumination, generates plantlet, wait long to 3-5 centimetres detection virus of buds, detected virus-free rear switching and enter fast numerous stage training
It supports, detected confirmation without virus, so that it may which the MS fast breeding culture medium progress for tapping into BA concentration 1mg/L, NAA concentration 0.1mg/L is fast
Numerous, bud is cut one by one when sprout length to 3 centimetres of sizes and is divided into single plant, cuts top by the long budding clump of one month or so stem apex
Blade is inoculated into the MS proliferated culture medium of BA concentration 1mg/L, NAA concentration 0.1mg/L and continues to be proliferated, and every bottle puts 5 buds, often
15 days or so 1 generations of proliferation；
(2) strong seedling culture: culture medium gradually reduces hormone concentration, and proliferation seedling is inoculated into strong seedling culture base when 6 generations bred
Middle culture；
(3) increase illumination: increasing illumination during strong sprout, illumination increases to 6000 luxs from 4000 luxs；
(4) culture of rootage: strong sprout, which is bred, is followed by root media 1/2MS, NAA0.1mg/L, in AC content 0.1%, chooses
Selecting sturdy young plant to be connected to root media is that it is promoted to take root, and weak young plant continues to be connected to strong sprout in strong seedling culture base；
(5) it is tamed before bottle outlet: the sweet potato detoxic seedling after taking root is transplanted in greenhouse, hardening 15 days, 18 degree of temperature, illumination
It, can cleaning and sterilizing plantation finishing scouring seedling 3 days after being opened after 20 days no more than 8000 luxs；
(6) it is managed after transplanting: being uniformly mixed with vermiculite, perlite, the dregs of a decoction, the turf that ratio is 1:1:1:3, be layered on 1.2
On the wide seedbed of rice, 30 centimetres of seedbed interval is sprinkled profoundly water, then after being sterilized with fungicide, plants ipomoea batatas seedling, 23 degree of curing temperature, wet
Degree 60%, 5000 lux of illumination is gradually increased after one month less than 10000 luxs.
Embodiment 2
A kind of sweet potato detoxication and tissue culture strong sprout method, specifically includes the following steps:
(1) detoxification treatment: the healthy and strong disease-free sweet potato of choosing takes 3 centimetres of stem apexs to sterilize on superclean bench, nothing as parent
Stem apex removing is carried out with dissecting needle under anatomical lens after bacterium processing, the shoot tip meristem of 0.3mm is cut, is seeded in 1/2MS,
On the solid medium of NAA0.1mg/L, it is sent between culture and air-dries, it is desirable that 25-30 DEG C of temperature, daily 12h, 4000 luxs
It is cultivated under illumination, generates plantlet, wait long to 3-5 centimetres detection virus of buds, detected virus-free rear switching and enter fast numerous stage training
It supports, detected confirmation without virus, so that it may which the MS fast breeding culture medium progress for tapping into BA concentration 1mg/L, NAA concentration 0.1mg/L is fast
Numerous, bud is cut one by one when sprout length to 3 centimetres of sizes and is divided into single plant, cuts top by the long budding clump of one month or so stem apex
Blade is inoculated into the MS proliferated culture medium of BA concentration 1mg/L, NAA concentration 0.1mg/L and continues to be proliferated, and every bottle puts 5 buds, often
25 days or so 1 generations of proliferation；
(2) strong seedling culture: culture medium gradually reduces hormone concentration, and proliferation seedling is inoculated into strong seedling culture base when 7 generations bred
Middle culture；
(3) increase illumination: increasing illumination during strong sprout, illumination increases to 6000 luxs from 4000 luxs；
(4) culture of rootage: strong sprout, which is bred, is followed by root media 1/2MS, NAA0.1mg/L, in AC content 0.1%, chooses
Selecting sturdy young plant to be connected to root media is that it is promoted to take root, and weak young plant continues to be connected to strong sprout in strong seedling culture base；
(5) it is tamed before bottle outlet: the sweet potato detoxic seedling after taking root is transplanted in greenhouse, hardening 18 days, 24 degree of temperature, illumination
It, can cleaning and sterilizing plantation finishing scouring seedling 4 days after being opened after 20 days no more than 8000 luxs；
(6) it is managed after transplanting: being uniformly mixed with vermiculite, perlite, the dregs of a decoction, the turf that ratio is 1:1:1:3, be layered on 1.2
On the wide seedbed of rice, 30 centimetres of seedbed interval is sprinkled profoundly water, then after being sterilized with fungicide, plants ipomoea batatas seedling, 28 degree of curing temperature, wet
Degree 80%, 7000 lux of illumination is gradually increased after one month less than 10000 luxs.
Embodiment 3
A kind of sweet potato detoxication and tissue culture strong sprout method, specifically includes the following steps:
(1) detoxification treatment: the healthy and strong disease-free sweet potato of choosing takes 5 centimetres of stem apexs to sterilize on superclean bench, nothing as parent
Stem apex removing is carried out with dissecting needle under anatomical lens after bacterium processing, the shoot tip meristem of 0.4mm is cut, is seeded in 1/2MS,
On the solid medium of NAA0.1mg/L, it is sent between culture and air-dries, it is desirable that 25-30 DEG C of temperature, daily 12h, 5000 luxs
It is cultivated under illumination, generates plantlet, wait long to 3-5 centimetres detection virus of buds, detected virus-free rear switching and enter fast numerous stage training
It supports, detected confirmation without virus, so that it may which the MS fast breeding culture medium progress for tapping into BA concentration 1mg/L, NAA concentration 0.1mg/L is fast
Numerous, bud is cut one by one when sprout length to 3 centimetres of sizes and is divided into single plant, cuts top by the long budding clump of one month or so stem apex
Blade is inoculated into the MS proliferated culture medium of BA concentration 1mg/L, NAA concentration 0.1mg/L and continues to be proliferated, and every bottle puts 5 buds, often
25 days or so 1 generations of proliferation；
(2) strong seedling culture: culture medium gradually reduces hormone concentration, and proliferation seedling is inoculated into strong seedling culture base when 8 generations bred
Middle culture；
(3) increase illumination: increasing illumination during strong sprout, illumination increases to 6000 luxs from 4000 luxs；
(4) culture of rootage: strong sprout, which is bred, is followed by root media 1/2MS, NAA0.1mg/L, in AC content 0.1%, chooses
Selecting sturdy young plant to be connected to root media is that it is promoted to take root, and weak young plant continues to be connected to strong sprout in strong seedling culture base；
(5) it is tamed before bottle outlet: the sweet potato detoxic seedling after taking root is transplanted in greenhouse, hardening, 20 days, temperature, 28 degree, light
It, can cleaning and sterilizing plantation finishing scouring seedling 4 days after being opened after 20 days according to 8000 luxs are no more than；
(6) it is managed after transplanting: being uniformly mixed with vermiculite, perlite, the dregs of a decoction, the turf that ratio is 1:1:1:3, be layered on 1.2
On the wide seedbed of rice, 30 centimetres of seedbed interval is sprinkled profoundly water, then after being sterilized with fungicide, plants ipomoea batatas seedling, curing temperature, 30 degree are wet
Degree, 90%, illumination, 8000 luxs are gradually increased after one month less than 10000 luxs.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (3)
1. a kind of sweet potato detoxication and tissue culture strong sprout method, which is characterized in that specifically includes the following steps:
(1) detoxification treatment: the healthy and strong disease-free sweet potato of choosing takes 1-5 centimetres of stem apex to sterilize on superclean bench as parent, sterile
Stem apex removing is carried out with dissecting needle under anatomical lens after processing, the shoot tip meristem of 0.2~0.4mm is cut, is seeded in 1/2MS,
On the solid medium of NAA0.1mg/L, it is sent between cultivating and air-dries, it is desirable that 25-30 DEG C of temperature, daily 12h, 4000~5000 Le
Cultivated under gram this illumination, generate plantlet, wait long to 3-5 centimetres detection virus of buds, detect it is virus-free after switching enter it is numerous fastly
Stage culture detected confirmation without virus, so that it may tap into the MS fast breeding culture medium of BA concentration 1mg/L, NAA concentration 0.1mg/L
Progress is fast numerous, and bud is cut one by one when sprout length to 3 centimetres of sizes and be divided into single plant, cut by the long budding clump of one month or so stem apex
Upper blade is gone, is inoculated into the MS proliferated culture medium of BA concentration 1mg/L, NAA concentration 0.1mg/L and continues to be proliferated, every bottle puts 5
Bud, every 15-25 days or so 1 generations of proliferation；
(2) strong seedling culture: culture medium gradually reduces hormone concentration, and 6-8 generation is inoculated into proliferation seedling in strong seedling culture base when breeding
Culture；
(3) increase illumination: increasing illumination during strong sprout, illumination increases to 6000 luxs from 4000 luxs；
(4) culture of rootage: strong sprout, which is bred, is followed by root media 1/2MS, NAA0.1mg/L, in AC content 0.1%, selects thick
It is that it is promoted to take root that strong sprout, which is connected to root media, and weak young plant continues to be connected to strong sprout in strong seedling culture base；
(5) it tames: the sweet potato detoxic seedling after taking root being transplanted in greenhouse, hardening 15-20 days before bottle outlet, temperature 18-28 degree, light
It, can cleaning and sterilizing plantation finishing scouring seedling 3~4 days after being opened after 20 days according to 8000 luxs are no more than；
(6) it is managed after transplanting: being uniformly mixed, be layered on 1.2 meters wide with vermiculite, perlite, the dregs of a decoction, the turf that ratio is 1:1:1:3
On seedbed, 30 centimetres of seedbed interval is sprinkled profoundly water, then after being sterilized with fungicide, plants ipomoea batatas seedling, curing temperature 23-30 degree, humidity
The lux 60-90%, illumination 5000-8000, gradually increases after one month less than 10000 luxs.
2. a kind of sweet potato detoxication and tissue culture strong sprout method according to claim 1, it is characterised in that: the strong seedling culture base
For 1/2MS culture medium, cycocel content 0.2mg/L, NAA content 0.05mg/L.
3. a kind of sweet potato detoxication and tissue culture strong sprout method according to claim 1, it is characterised in that: the root media
It is 1/2MS culture medium, NAA content 0.1mg/L, AC content 0.1%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113728921A (en) * | 2021-08-10 | 2021-12-03 | 云南省农业科学院花卉研究所 | Tissue culture propagation method for Chimonanthus praecox |
| CN115812598A (en) * | 2022-12-07 | 2023-03-21 | 江苏丰收大地种业发展有限公司 | Liquid culture method for sweet potato detoxified tissue culture seedlings by taking sponge as support |
| CN117652254A (en) * | 2023-12-04 | 2024-03-08 | 湖北薯芋产业技术研究院有限公司 | Detoxification preservation method of sweet potato seeds |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05260869A (en) * | 1992-02-10 | 1993-10-12 | Iseki & Co Ltd | Adventitious root of sweet potato, its inducing method and its culture method |
| JP2006141274A (en) * | 2004-11-19 | 2006-06-08 | Oita General Service Kk | Method for culturing shoot apex |
| CN101584301A (en) * | 2009-06-05 | 2009-11-25 | 海南省农业科学院粮食作物研究所 | The method of culturing detoxified seedling by sweet potato stem tip |
| CN101611697A (en) * | 2009-07-13 | 2009-12-30 | 周玉玲 | Sweet potato merchant 19 detoxifying fast breeding technique and cultivation material |
| CN107455262A (en) * | 2017-09-26 | 2017-12-12 | 牛力立 | A kind of plant modification method of detoxification red heart" sweet potato |
-
2018
- 2018-10-24 CN CN201811242428.1A patent/CN109197590A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05260869A (en) * | 1992-02-10 | 1993-10-12 | Iseki & Co Ltd | Adventitious root of sweet potato, its inducing method and its culture method |
| JP2006141274A (en) * | 2004-11-19 | 2006-06-08 | Oita General Service Kk | Method for culturing shoot apex |
| CN101584301A (en) * | 2009-06-05 | 2009-11-25 | 海南省农业科学院粮食作物研究所 | The method of culturing detoxified seedling by sweet potato stem tip |
| CN101611697A (en) * | 2009-07-13 | 2009-12-30 | 周玉玲 | Sweet potato merchant 19 detoxifying fast breeding technique and cultivation material |
| CN107455262A (en) * | 2017-09-26 | 2017-12-12 | 牛力立 | A kind of plant modification method of detoxification red heart" sweet potato |
Non-Patent Citations (6)
| Title |
|---|
| MASEKESA RT ET AL.: "Effect of BAP, NAA and GA3, either alone or in combination, on meristem culture and plantlet establishment in sweet potato (cv Brondal)", 《AFRICAN JOURNAL OF FOOD, AGRICULTURE, NUTRITION AND DEVELOPMENT》 * |
| Q.C.WANG ET AL.: "Elimination of two viruses which interact synergistically from sweetpotato by shoot tip culture and cryotherapy", 《JOURNAL OF VIROLOGICAL METHODS》 * |
| 朱红彩 等: "河南省脱毒甘薯茎尖培养与快繁技术", 《农业科技通讯》 * |
| 林丛发 等: "紫甘薯福宁紫3号茎尖离体培养及种薯生产", 《农业科技通讯》 * |
| 王玉英 等: "《植物组织培养技术手册》", 31 March 2006, 金盾出版社 * |
| 胡颂平 等: "《植物细胞组织培养技术》", 31 August 2014, 中国农业大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113728921A (en) * | 2021-08-10 | 2021-12-03 | 云南省农业科学院花卉研究所 | Tissue culture propagation method for Chimonanthus praecox |
| CN113728921B (en) * | 2021-08-10 | 2023-07-28 | 云南省农业科学院花卉研究所 | Chimonanthus praecox tissue culture propagation method |
| CN115812598A (en) * | 2022-12-07 | 2023-03-21 | 江苏丰收大地种业发展有限公司 | Liquid culture method for sweet potato detoxified tissue culture seedlings by taking sponge as support |
| CN117652254A (en) * | 2023-12-04 | 2024-03-08 | 湖北薯芋产业技术研究院有限公司 | Detoxification preservation method of sweet potato seeds |
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