CN104782487A - Detoxification and cell pan rapid propagation method for purple sweet potatoes - Google Patents
Detoxification and cell pan rapid propagation method for purple sweet potatoes Download PDFInfo
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Abstract
The invention discloses a detoxification and cell pan rapid propagation method for purple sweet potatoes and belongs to the technical field of crop variety breeding. The method comprises the following steps: disinfecting stem tips of the purple sweet potatoes; peeling and inoculating meristems of the stem tips of the purple sweet potatoes; culturing the meristems of the stem tips of the purple sweet potatoes; selecting the cell pan and a substrate; transplanting and rapidly propagating a virus-free tube seedling of the purple sweet potatoes and the like. According to the method disclosed by the invention, the formula of a detoxification and regeneration culture medium of purple sweet potatoes is improved, and the method is convenient to operate and great in inoculum size. By using the cell pan, the rapid propagation speed of a virus-free seedling is greatly accelerated. Furthermore, the method is high in propagation coefficient and is not limited by seasons.
Description
Technical field
The present invention relates to the tissue culture detoxicating of a Plants and fast numerous field, be specifically related to a kind of purple sweep potato detoxification and the numerous soon method of cave dish.
Background technology
Purple sweep potato refers to the class sweet potato of potato block yellowish pink in purple, its rich in nutritive value, except the nutritive value with Ordinary Sweet Potatoes, also containing abundant anthocyan.Research shows, cyanine in purple potato block root have very strong antioxidation, active oxygen can be removed, preventing hypertension and arteriosclerosis, improve liver function, reduces gene mutation, suppress the generation of carcinogen, improve the health-care effects such as eyesight, and purple sweet potato anthocyanin also has good heat-resisting light resistance, food, cosmetics, medical in also have broad application prospects.Therefore purple sweep potato also more and more welcomed by the people is planted in recent years.But along with the expansion of purple planting potato area, purple potato is also serious all the more by the harm of virus, and purple potato causes deterioration of variety after infecting virus, and death rate increases, and production declining even has no harvest; The purple potato potato block infecting virus diminishes, quality variation.Purple potato virus disease has become the major reason causing sweet potato kind sexual involution, production declining, only has and carries out the virus-free culture of shoot apical meristem and numerous soon, the yield and quality of the former purple potato kind of guarantee to purple potato seedling of catching an illness.
In recent years, along with the continuous progress of plant tissue culture technique, tissue cultures is utilized also to be widely used on vegetables, flowers and some crops the technology that plant carries out detoxification.But the growth characteristics that different plants has them different, detoxification and concrete grammar numerous are soon also different because of plant, different because of kind.Therefore, purple sweep potato is because of the feature of himself, and virus-free culture and technology numerous soon are also different from other plant, needs researcher constantly to explore and perfect by testing.
Summary of the invention
Quick-breeding method is coiled in the detoxification and the cave that the object of this invention is to provide a kind of purple sweep potato, both can ensure former purple potato variety yield and level of quality, can break again restriction in season, and realization is expanded numerous repeatedly, efficiently.
Object of the present invention is achieved by the following technical programs:
A quick-breeding method is coiled in the detoxification of purple sweep potato and cave, comprises the steps:
1) stem apex sterilization: the stem apex cutting about 2cm from purple sweep potato seedling, put into clean triangular flask after removing all blades, under running water, repeatedly rinse 5 ~ 10min, add two liquid detergents and soak 10min, remove cleaning solution, with distilled water cleaning 4 ~ 5 times.Transferred to by stem apex on superclean bench, with 75% alcohol immersion 30s, by sterile water wash 2 ~ 3 times, then NaClO solution stem apex being placed in 2% soaks 10min, or the HgCl of 0.1%
2soak 5min in (mercuric chloride) solution, use aseptic water washing afterwards 3 times, the stem apex aseptic filter paper after sterilization sucks the moisture on stem apex surface, is placed in sterile petri dish for subsequent use;
2) stripping of shoot apical meristem and inoculation: under sterile stem apex being placed in 20-80 times of stereomicroscope (bitubular anatomical lens), successively spire is removed with dissecting needle, until expose smooth shoot apical meristem, the growing point of band 1 ~ 2 leaf primordium (0.1 ~ 0.3mm) is cut with scalpel, growing point moved in the culture dish that regeneration culture medium is housed with transfer needle and cultivate, about 20 growing points in each culture dish, can be inoculated;
3) cultivation of shoot apical meristem: postvaccinal culture dish is placed on illumination box or organizes on the indoor illumination cultivation frame of training and cultivate.Condition of culture is temperature 25 DEG C, intensity of illumination 2000 ~ 3000lx, light application time 16h/d, general cultivation 20 ~ 30d sees that stem apex obviously extends and in green, when having leaflet to occur, little stem eye is proceeded in vitro cultivating of subculture medium, continued growth also forms root system, then can develop into the plantlet of 4 ~ 5 blades through about 1 month; Detect Regenerated plantlet by serum method, remove the plant of carrying sweet potato viruses.
4) cave dish and matrix is prepared: select vinyon cave dish, be of a size of 54cm × 28cm, specification 50 cave (bore 50mm × 50mm, the dark 50mm in cave) or 72 caves (bore 40mm × 40mm, the dark 45mm in cave) is advisable; Soak 12 hours with 500 times of carbendazols or steep 30 minutes by potassium permanganate 1000 times of immersions, drying after then cave dish being cleaned up with clear water; Fill matrix in being coiled in cave, matrix selects Nutrition Soil, or autogamy (every 100kg loess+high temperature puffing chicken manure 5kg+45% Nitrogen, Phosphorus and Potassium 1kg+50% carbendazim 50g), and water permeable for subsequent use, cave tray bottom is used at the bottom of tray pad.
5) transplanting of detoxification test tube plantlet: carefully intercept the stem section being about about 4cm by the test-tube plantlet of Viral diagnosis in superclean bench with scalpel, each stem section is removed the blade of bottom, only stay 1, upper end expanded leaves, insert in a hole of cave dish, insertion depth about 2cm, the remaining part of test-tube plantlet root still stays continued growth in test tube; Treat that test-tube plantlet grows to about 6cm Shi Ke and again intercepts top stem section transplanting in the dish of cave, repeatedly carry out always.
6) the cave dish being plugged seedling is put on Multilayer culture shelf of greenhouse or booth, enclosed fly net; Control the temperature of greenhouse or booth at 25 ~ 28 DEG C, minimumly lower than 20 DEG C, can not notice that timing is watered to potato seedling.
7) again seedling can be cut when potato seedling grows to about 10cm, the upper half only stays 1 expanded leaves topmost, remove the unnecessary blade in bottom, insert in the cave dish made new advances, second is trapped in continued growth in original cave dish, so carry out continuously cutting the fast-propagation that seedling can realize Sweetpotato Viruses Elimination seedling, at any time for field-transplanting.
The detoxification of aforementioned purple sweep potato and cave are coiled in quick-breeding method, the proportioning of described regeneration culture medium is: MS minimal medium powder 4.4g/L, 6-benzyladenine (6-BA) 1.0 ~ 2.0mg/L, a-naa (NAA) 0.1 ~ 1.0mg/L, sucrose 30g/L, plant gel Gelzan 3g/L; The proportioning of subculture medium is: MS minimal medium powder 4.4g/L, sucrose 30g/L, plant gel Gelzan 3g/L.
Good effect of the present invention is:
1. conventional vaccination mode is all with test tube, often manage and only can inoculate 1 shoot apical meristem, and utilize culture dish to inoculate, more convenient operation (the culture dish degree of depth is more shallow), single ware inoculum concentration large (about 20/ware), culture dish can stack in illumination box or group training room in layering, saves culture space greatly;
2. utilize cave dish method to carry out the fast-propagation of detoxic seedling, easy and simple to handle, reproduction coefficient is high, is not subject to seasonal restrictions.
3. pair purple potato kind of contamination is carried out detoxification and expands numerous, effectively prevents deterioration of variety, keeps kind of property and ensures stable yields and quality.
Embodiment
By following examples, the present invention is described in further detail, but content of the present invention is not limited thereto.
The experimental technique that experimental technique below in embodiment uses, is conventional method if no special instructions; The material used in embodiment below, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1
Select state to examine purple sweep potato kind " peaceful No. 1, purple potato " and carry out detoxification and numerous soon, concrete steps are as follows:
1) stem apex sterilization: mid-April chooses the healthy potato seedling with this kind characteristic feature, robust growth, anosis worm on " peaceful No. 1, purple potato " sweet potato seedbed, the stem apex of clip potato seedling top about 2cm, remove all visible leaves, put into clean triangular flask, repeatedly rinse 5min under running water after, add two liquid detergents and soak 10min, remove cleaning solution, clean 5 times with distilled water again, clean residual cleaning solution.Subsequently stem apex is transferred on superclean bench, surface sterilization is carried out with 75% alcohol immersion 30s, by sterile water wash 2 times, the NaClO solution again stem apex being placed in 2% soak 10min after with aseptic water washing 3 times, clean residual NaClO solution, carry out degree of depth sterilization, the stem apex aseptic filter paper after sterilization sucks the moisture on stem apex surface, is placed in sterile petri dish for subsequent use;
2) stripping of shoot apical meristem and inoculation: under sterile stem apex being placed in 80 times of Stereo microscopes (bitubular anatomical lens), successively spire is removed with dissecting needle, until expose smooth shoot apical meristem, the growing point with 1 ~ 2 leaf primordium (0.1 ~ 0.3mm) is carefully cut with scalpel, with transfer needle growing point moved to and regeneration culture medium (MS minimal medium powder 4.4g/L is housed, 6-benzyladenine 1.0mg/L, a-naa 0.1mg/L, sucrose 30g/L, plant gel Gelzan 3g/L) culture dish in cultivate, 20 growing points can be inoculated in each culture dish,
3) cultivation of shoot apical meristem: postvaccinal culture dish is placed on the indoor illumination cultivation frame of group training and cultivates.Condition of culture is temperature 25 ~ 28 DEG C, intensity of illumination 2000 ~ 3000lx, light application time 16h/d, after cultivating 20d, when seeing that stem apex obviously extends and in green, when having leaflet to occur, little stem eye is proceeded in vitro cultivating of subculture medium (MS minimal medium powder 4.4g/L, sucrose 30g/L, plant gel Gelzan 3g/L), make its continued growth and form root system, a seedling planted by each test tube, can develop into " peaceful No. 1, purple potato " sweet potato plantlet of 4 ~ 5 blades through about 30d;
4) the sick seedling of test-tube plantlet detects: peaceful purple potato No. 1 test-tube plantlet born again will carry out Viral diagnosis in time, and the test-tube plantlet generally growing to 4 ~ 5 leaf blade size can carry out Viral diagnosis.Band poison test-tube plantlet blade having the bright arteries and veins of floral leaf or chlorisis spot is first removed by ocular estimate; The test-tube plantlet serum method detection that ocular estimate is fubaritic, removes the test-tube plantlet carrying Sweet Potato Feathery Mottle Virus, sweet potato cryptovirus, sweet potato class mosaic virus.
5) prepare cave dish and matrix: select to be of a size of 54cm × 28cm, the vinyon cave dish in specification 50 cave, first soaks 12 hours with 500 times of carbendazols before using, and then with clear water, cave dish is cleaned up and is dried; Fill matrix in being coiled in cave, matrix selects Nutrition Soil (every 100kg loess+high temperature puffing chicken manure 5kg+45% Nitrogen, Phosphorus and Potassium 1kg+50% carbendazim 50g), and water permeable for subsequent use, cave tray bottom is used at the bottom of tray pad, prevents moisture from overflowing.
6) transplanting of detoxification test tube plantlet: carefully intercept the stem section being about about 4cm by peaceful purple potato No. 1 test-tube plantlet of Viral diagnosis in superclean bench with scalpel, each stem section is removed the blade of bottom, only stay 1, upper end expanded leaves, insert in a hole of cave dish, insertion depth about 2cm, the remaining part of test-tube plantlet root still stays continued growth in test tube; Treat that test-tube plantlet again grows to about 6cm Shi Ke and again intercepts top stem section transplanting in the dish of cave, repeatedly carry out always.
7) the cave dish being plugged peaceful purple potato No. 1 detoxic seedling is put on Multilayer culture shelf of booth, enclosed fly net; Control the temperature of greenhouse or booth at 25 ~ 28 DEG C, timing waters to potato seedling.
8) again seedling can be cut when potato seedling grows to about 10cm, the upper half only stays 1 ~ 2 expanded leaves topmost, remove the unnecessary blade in bottom, insert in new cave dish, second is trapped in continued growth in original cave dish, so carry out continuously cutting seedling, the Fast-propagation of 8 ~ 16 times can be realized general every month, at any time for field-transplanting.
This kind is spring, summer potato type, adapts to planting range wide, output 30000 ~ 45000kg/hm
2; Than contrast (No. 1, peaceful purple potato before detoxification) volume increase 28.7% after detoxification.
Embodiment 2
Select " peaceful No. 2, purple potato " purple sweep potato kind carry out detoxification and cave dish numerous soon, concrete steps are as follows:
1) stem apex sterilization: mid-April chooses the healthy potato seedling with this kind characteristic feature, robust growth, anosis worm on " peaceful No. 2, purple potato " purple sweep potato seedbed, the stem apex of clip potato seedling top about 2cm, remove all visible leaves, put into clean triangular flask, repeatedly rinse 10min under running water after, add two liquid detergents and soak 10min, remove cleaning solution, clean 5 times with distilled water again, clean residual cleaning solution.Subsequently stem apex is transferred on superclean bench, surface sterilization is carried out with 75% alcohol immersion 30s, by sterile water wash 2 times, the NaClO solution again stem apex being placed in 2% soaks 10min, use aseptic water washing afterwards 3 times, clean residual NaClO solution, carry out degree of depth sterilization, stem apex aseptic filter paper after sterilization sucks the moisture on stem apex surface, is placed in sterile petri dish for subsequent use.
2) stripping of shoot apical meristem and inoculation: under sterile stem apex being placed in 80 times of stereomicroscopes (bitubular anatomical lens), successively spire is removed with dissecting needle, until expose smooth shoot apical meristem, the growing point with 1 ~ 2 leaf primordium (0.1 ~ 0.3mm) is carefully cut with scalpel, with transfer needle growing point moved to and regeneration culture medium (MS minimal medium powder 4.4g/L is housed, 6-benzyladenine 2.0mg/L, a-naa 0.5mg/L, sucrose 30g/L, plant gel Gelzan 3g/L) culture dish in cultivate, about 20 growing points can be inoculated in each culture dish.
3) cultivation of shoot apical meristem: postvaccinal culture dish is placed on illumination box and cultivates.Condition of culture is temperature 25 ~ 28 DEG C, intensity of illumination 2000 ~ 3000lx, light application time 16h/d, after cultivating 30d, when seeing that stem apex obviously extends and in green, when having leaflet to occur, little stem eye is proceeded in vitro cultivating of subculture medium (MS minimal medium powder 4.4g/L, sucrose 30g/L, plant gel Gelzan 3g/L), make its continued growth and form root system, a seedling planted by each test tube, generally can develop into " peaceful No. 2, purple potato " plantlet of 4 ~ 5 blades through about 30d.
4) the sick seedling of test-tube plantlet detects: " peaceful No. 2, purple potato " the purple sweep potato test-tube plantlet born again will carry out Viral diagnosis in time, and the test-tube plantlet generally growing to 4 ~ 5 leaf blade size can carry out Viral diagnosis.Band poison test-tube plantlet blade having the bright arteries and veins of floral leaf or chlorisis spot is first removed by ocular estimate; The test-tube plantlet serum method detection that ocular estimate is fubaritic, removes the test-tube plantlet carrying Sweet Potato Feathery Mottle Virus, sweet potato cryptovirus, sweet potato class mosaic virus.
5) prepare cave dish and matrix: select to be of a size of 54cm × 28cm, the vinyon cave dish in specification 72 cave, first steeps 30 minutes by potassium permanganate 1000 times of immersions before using, and then with clear water, cave dish is cleaned up and is dried; Fill matrix in being coiled in cave, matrix autogamy (every 100kg loess+high temperature puffing chicken manure 5kg+45% Nitrogen, Phosphorus and Potassium 1kg+50% carbendazim 50g), water permeable for subsequent use, cave tray bottom is used at the bottom of tray pad, prevents moisture from overflowing.
6) transplanting of detoxification test tube plantlet: carefully intercept the stem section being about about 4cm by " peaceful No. 2, purple potato " purple sweep potato test-tube plantlet of Viral diagnosis in superclean bench with scalpel, each stem section is removed the blade of bottom, only stay 1, upper end expanded leaves, insert in a hole of cave dish, insertion depth about 2cm, the remaining part of test-tube plantlet root still stays continued growth in test tube; Treat that test-tube plantlet again grows to about 6cm Shi Ke and again intercepts top stem section transplanting in the dish of cave, repeatedly carry out always.
7) the cave dish being plugged peaceful purple potato No. 1 detoxic seedling is put into greenhouse, enclose fly net; Control the temperature in greenhouse at 25 ~ 28 DEG C, timing waters to potato seedling.
8) again seedling can be cut when potato seedling grows to about 10cm, the upper half only stays 1 ~ 2 expanded leaves topmost, remove the unnecessary blade in bottom, insert in new cave dish, second is trapped in continued growth in original cave dish, so carry out continuously cutting seedling, the Fast-propagation of 10 ~ 16 times can be realized general every month, at any time for field-transplanting.
" peaceful No. 2, purple potato " purple sweep potato kind after detoxification is than " peaceful No. 2, purple potato " the purple sweep potato kind volume increase 30.2% before detoxification.
Embodiment 3
Purple sweep potato kind carries out detoxification and cave dish is numerous soon to select " purple No. 1, the potato in Zhejiang ", and concrete steps are as follows:
1) stem apex sterilization: the stem apex cutting about 2cm from " purple No. 1, the potato in Zhejiang " purple sweep potato seedling, put into clean triangular flask after removing all blades, under running water, repeatedly rinse 8min, add two liquid detergents and soak 10min, remove cleaning solution, clean 5 times with distilled water.Stem apex is transferred on superclean bench, with 75% alcohol immersion 30s, by sterile water wash 3 times, then stem apex is placed in the HgCl of 0.1%
2soak 5min in (mercuric chloride) solution, use aseptic water washing afterwards 3 times, the stem apex aseptic filter paper after sterilization sucks the moisture on stem apex surface, is placed in sterile petri dish for subsequent use;
2) stripping of shoot apical meristem and inoculation: under sterile stem apex being placed in 60 times of stereomicroscopes (bitubular anatomical lens), successively spire is removed with dissecting needle, until expose smooth shoot apical meristem, the growing point of band 1 ~ 2 leaf primordium (0.1 ~ 0.3mm) is cut with scalpel, with transfer needle growing point moved to and regeneration culture medium is housed (proportioning is: MS minimal medium powder 4.4g/L, 6-benzyladenine 1.5mg/L, a-naa 1mg/L, sucrose 30g/L, plant gel Gelzan 3g/L; ) culture dish in cultivate, about 20 growing points can be inoculated in each culture dish;
3) cultivation of shoot apical meristem: postvaccinal culture dish is placed on illumination box or organizes on the indoor illumination cultivation frame of training and cultivate.Condition of culture is temperature 25 DEG C, intensity of illumination 2500lx, light application time 16h/d, cultivate and to see that stem apex obviously extends and in green after 25d, (proportioning is: MS minimal medium powder 4.4g/L when having leaflet to occur, little stem eye to be proceeded to subculture medium, sucrose 30g/L, plant gel Gelzan 3g/L) in vitro cultivate, continued growth also forms root system, then can develop into the plantlet of 4 ~ 5 blades through about 1 month; Detect Regenerated plantlet by serum method, remove the plant of carrying sweet potato viruses.
4) cave dish and matrix is prepared: select vinyon cave dish, be of a size of 54cm × 28cm, specification 72 cave (bore 40mm × 40mm, the dark 45mm in cave); Steep 30 minutes by potassium permanganate 1000 times of immersions, dry after then cave dish being cleaned up with clear water; Fill matrix in being coiled in cave, matrix selects Nutrition Soil, or autogamy (every 100kg loess+high temperature puffing chicken manure 5kg+45% Nitrogen, Phosphorus and Potassium 1kg+50% carbendazim 50g), and water permeable for subsequent use, cave tray bottom is used at the bottom of tray pad.
5) transplanting of detoxification test tube plantlet: carefully intercept the stem section being about about 4cm by the test-tube plantlet of Viral diagnosis in superclean bench with scalpel, each stem section is removed the blade of bottom, only stay 1, upper end expanded leaves, insert in a hole of cave dish, insertion depth about 2cm, the remaining part of test-tube plantlet root still stays continued growth in test tube; Treat that test-tube plantlet grows to about 6cm Shi Ke and again intercepts top stem section transplanting in the dish of cave, repeatedly carry out always.
6) the cave dish being plugged seedling is put on Multilayer culture shelf of greenhouse or booth, enclosed fly net; Control the temperature of greenhouse or booth at 25 ~ 28 DEG C, timing waters to potato seedling.
7) again seedling can be cut when potato seedling grows to about 10cm, the upper half only stays 1 expanded leaves topmost, remove the unnecessary blade in bottom, insert in the cave dish made new advances, second is trapped in continued growth in original cave dish, so carry out continuously cutting seedling, the Fast-propagation of 10 ~ 18 times can be realized general every month, at any time for field-transplanting.
" purple No. 1, the potato in Zhejiang " purple sweep potato kind after detoxification is than " Zhejiang purple No. 1, potato " the purple sweep potato kind volume increase 25.5% before detoxification.
Claims (5)
1. a method numerous is soon coiled in purple sweep potato detoxification and cave, it is characterized in that carrying out as follows:
1) purple potato stem apex sterilization: the stem apex cutting about 2cm from purple sweep potato seedling, removes all blades, under running water, repeatedly rinses 5 ~ 10min, add two liquid detergents and soak 10min, remove cleaning solution, with distilled water cleaning 4 ~ 5 times; Stem apex is transferred on superclean bench, with 75% alcohol immersion 30s, by sterile water wash 2 ~ 3 times, the NaClO solution again stem apex being placed in 2% soaks 10min, or soak 5min in the mercuric chloride solution of 0.1%, use aseptic water washing afterwards 3 times, the stem apex aseptic filter paper after sterilization sucks the moisture on stem apex surface, is placed in sterile petri dish for subsequent use;
2) stripping of purple potato shoot apical meristem and inoculation: under sterile stem apex being placed in 20 ~ 80 times of stereomicroscopes, successively spire is removed with dissecting needle, until expose smooth shoot apical meristem, the growing point of the long leaf primordium of band 1 ~ 2 0.1 ~ 0.3mm is cut with scalpel, growing point moved in the culture dish that regeneration culture medium is housed with transfer needle and cultivate, inoculation 20 growing points in each culture dish;
3) cultivation of purple potato shoot apical meristem: postvaccinal culture dish is placed on illumination box or organizes on the indoor illumination cultivation frame of training and cultivate, condition of culture is temperature 25 DEG C, intensity of illumination 2000 ~ 3000lx, light application time 16h/d, cultivate 20 ~ 30d and see that stem apex obviously extends and in green, when having leaflet to occur, little stem eye is proceeded in vitro cultivating of subculture medium, continued growth also forms root system, then can develop into the plantlet of 4 ~ 5 blades through about 1 month; Detect Regenerated plantlet by serum method, remove the plant of carrying sweet potato viruses;
4) prepare cave dish and matrix: select vinyon cave dish, soak 12 hours with 500 times of carbendazols or steep 30 minutes by potassium permanganate 1000 times of immersions, dry after then cave dish being cleaned up with clear water; Fill matrix in being coiled in cave, water permeable for subsequent use, cave tray bottom is used at the bottom of tray pad;
5) transplanting of purple potato detoxification test tube plantlet: the stem section carefully intercepting test-tube plantlet about the 4cm by Viral diagnosis in superclean bench with scalpel, each stem section is removed the blade of bottom, only stay 1, upper end expanded leaves, insert in a hole of cave dish, insertion depth about 2cm, the remaining part of test-tube plantlet root still stays continued growth in test tube; Treat that test-tube plantlet grows to about 6cm Shi Ke and again intercepts top stem section transplanting in the dish of cave, repeatedly carry out always;
6) the cave dish being plugged seedling is put on Multilayer culture shelf of greenhouse or booth, enclosed fly net; Control the temperature of greenhouse or booth at 25 ~ 28 DEG C, timing waters to potato seedling;
7) again seedling can be cut when potato seedling grows to about 10cm, the upper half only stays 1 expanded leaves topmost, remove the unnecessary blade in bottom, insert in new cave dish, second is trapped in continued growth in original cave dish, so carry out continuously cutting the fast-propagation that seedling can realize Sweetpotato Viruses Elimination seedling, at any time for field-transplanting.
2. method numerous is soon coiled in purple sweep potato detoxification according to claim 1 and cave, it is characterized in that, the proportioning of the regeneration culture medium described in step (2) is: MS minimal medium powder 4.4g/L, 6-benzyladenine 1.0 ~ 2.0mg/L, a-naa 0.1 ~ 1.0mg/L, sucrose 30g/L, plant gel Gelzan 3g/L.
3. method numerous is soon coiled in purple sweep potato detoxification according to claim 1 and cave, it is characterized in that, the proportioning of the subculture medium described in step (3) is: MS minimal medium powder 4.4g/L, sucrose 30g/L, plant gel Gelzan 3g/L.
4. method numerous is soon coiled in purple sweep potato detoxification according to claim 1 and cave, it is characterized in that, the cave dish described in step (4) is of a size of 54cm × 28cm, specification 50 cave or 72 caves.
5. method numerous is soon coiled in purple sweep potato detoxification according to claim 1 and cave, and it is characterized in that, the substrate composition described in step (4) is: loess 100kg+ high temperature puffing chicken manure 5kg+45% Nitrogen, Phosphorus and Potassium 1kg+50% carbendazim 50g.
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| CN105706880A (en) * | 2016-03-15 | 2016-06-29 | 宁夏农林科学院 | Purple sweet potato virus-free seedling water culture fast breeding method |
| CN105900838A (en) * | 2016-04-25 | 2016-08-31 | 吉林师范大学 | Purple sweet potato detoxification method |
| CN106386494A (en) * | 2016-09-29 | 2017-02-15 | 紫云自治县紫香源农林科技有限责任公司 | Method for removing toxin and breeding seeds from stem apexes of sweet potatoes |
| CN106386144B (en) * | 2016-10-09 | 2020-03-31 | 江苏建康职业学院 | Cultivation method of traditional Chinese medicine virus-free test-tube plantlet |
| CN106386144A (en) * | 2016-10-09 | 2017-02-15 | 江苏建康职业学院 | Cultivation method of traditional Chinese virus-free test-tube plantlets |
| CN106961984A (en) * | 2017-04-19 | 2017-07-21 | 农业部南京农业机械化研究所 | A kind of bowl seeding cultivating method based on sweet potato vine |
| CN108901841A (en) * | 2018-06-26 | 2018-11-30 | 山东省农业科学院作物研究所 | Sweetpotato Viruses Elimination test tube seedling greenhouse green quick-breeding method |
| CN108901841B (en) * | 2018-06-26 | 2020-01-17 | 山东省农业科学院作物研究所 | Greenhouse green rapid propagation method for sweet potato virus-free test-tube plantlet |
| CN110352852A (en) * | 2019-08-22 | 2019-10-22 | 湖北省农业科学院粮食作物研究所 | A kind of sweet potato propagation method |
| CN114868613A (en) * | 2022-04-02 | 2022-08-09 | 洛阳农林科学院 | Rapid breeding method of detoxified sweet potatoes in one-mu field with one seedling |
| CN115644063A (en) * | 2022-11-11 | 2023-01-31 | 青岛农业大学 | Rapid propagation method of virus-free raw seedling of sweet potato |
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