KR20000046082A - Method for producing potato saplings grown up from stem cutting for growing in liquid nutrition medium and culture broth compositions(cheju b liquid medium) - Google Patents

Abstract

PURPOSE: A method for producing the potato saplings grown up from a stem cutting for growing in a liquid nutrition medium and culture broth compositions(Cheju B liquid medium) are provided for growing a large amount of potatoes without contamination and lowering the cost. CONSTITUTION: A culture broth composition(Cheju B liquid medium) for producing the potato saplings grown up from a stem cutting for growing in a liquid nutrition medium consists of KNO3, Ca(NO3)2-4H2O, NH4H2PO4, MgSO4-7H2O, Fe-EDTA, H3BO3, ZnSO4-4H2O, MnSO4-4H2O, CuSO4-5H2O, and (NH4)6Mo7O24. The method for growing potatoes in a liquid nutrition medium comprises the steps of: producing the potato saplings grown up from a stem cutting, examining the presence of virus, transferring 3 to 4 cm virus-free potato saplings into the soil with supplying with only water for 5 days; supplying the potato saplings with the liquid nutrition medium of the invention after 5 days of transportation; collecting the grown potato saplings after 10 days; stem cutting the potato saplings; transferring the sapling cuts and remained stem saplings into the the soil and supplying with only water for 5 days; supplying the potato saplings with the liquid nutrition medium after 5 days of transportation; and collecting the grown potato saplings after 10 to 15 days of second transportation.

Description

Potato stem pruning seed production method for nutrient cultivation and culture solution composition (Jeju sap)

The present invention relates to a method for producing potato stem pruning seedlings for nutrient cultivation, and more particularly, a method for producing potato stem pruning seedlings capable of rapidly growing in large quantities without contamination by transferring potato stalks produced by tissue culture to normal soil. It relates to a culture solution composition.

Nutrient cultivation is advantageous in that it is possible to continuously cultivate crops for a long time in the same place as compared to soil cultivation, does not need to replace the soil in the facility, and can achieve a constant yield every time without knowing the weather. In particular, because of the good working environment, it is possible to reduce the labor of agricultural machinery work, weeding, etc., it is possible to automate environmental management, and to increase the use of land or facility space, mass production of fresh and clean crops throughout the year. Moreover, crops produced by nutrient cultivation can be sold at a higher price because of the image of higher quality and sanitation than crops produced by soil cultivation.

Thus, even in the case of potatoes have been accumulated during the spray bottle or culture medium nutrient cultivation technology, proliferation techniques for securing a large amount of seedlings for this has been continuously researched and developed.

Conventionally, as a seedling production method for nutrient cultivation of potatoes, potato stems have been used as a tissue culture in flasks or culture bottles, or artificial seed potatoes and nutrient cultivation sows (about 1 g) have been used.

However, potato seedling production method by tissue culture requires a lot of tissue culture equipment in order to multiply in large quantities, and it is difficult to multiply at a time due to bottle contamination in management, for example, about three months to produce 10,000 seedlings. It takes

In addition, when the artificial seed potato and nutrient cultivation sows are used by germination, the size is small and weak in the environment during direct cultivation, so that the initial growth is poor, it is difficult to secure the same size seedlings at one time, and the production cost is high.

Thus, the inventors of the present invention, while exploring a method for securing a large amount of seedlings in a short time, in view of the limited number of times to grow the potato stem obtained by tissue culture in a flask or a culture bottle, We studied how to transfer them to soil. However, in the case of potatoes using the underground, seedling production in the top soil has a problem that is wiped out by decay. Because of this, potato seedling production has been largely dependent on tissue culture. However, the present inventors can significantly reduce the contamination caused by decay by supplying the nutrient solution of the specific composition developed by the inventors after rooting in the soil, and shows good results in the growth situation of the obtained primary seedlings, Based on this, the present invention has been completed.

In addition, as seedling production is possible in the soil, stem pruning can be applied and it can be continuously doubled in about 10 days, which can reduce production costs by 75% compared to tissue culture seedlings. It was.

It is therefore an object of the present invention to provide a nutrient solution composition which enables seedling production on topsoil without contamination.

Another object of the present invention is to provide a method for producing potato stem pruning seedlings capable of rapidly growing in large quantities without contamination.

1 is a photograph comparing the production of seedling stem by potato stem pruning seedling and tissue culture in the soil according to the present invention.

Figure 2a is a picture showing the seedling harvesting scene, seedlings cut the central stem, when using a surgical knife at this time.

2b is a photograph showing the folding of the collected seedlings.

Figure 2c is a photograph showing the rooting situation of the seedlings on the 5th and 15th day after the folding.

The nutrient solution composition (Jeju B liquid) for producing potato stem bark seedlings according to the present invention is characterized by including the following composition:

division Content (mg / l) Large amount of element KNO ₃ NH ₄ H ₂ PO ₄ Ca (NO ₃ ) ₂ 4H ₂ OMgSO ₄ 7H ₂ O 40578475250 Trace elements Fe-EDTAH ₃ BO ₃ MnSO ₄ 4H ₂ OZnSO ₄ 7H ₂ OCuSO ₄ 5H ₂ O (NH ₄ ) ₆ Mo ₇ O ₂₄ 20320.220.050.02

When explaining the production method of the stem bark seedling using the nutrient solution composition according to the present invention in more detail is characterized by including the following steps:

(1) production by tissue culture and virus assaying, harvesting seedlings around 3 to 4 cm free of viruses and transferring them to the top soil for 5 days, supplying only water and cultivating them without supplying nutrient solution;

(2) cultivating while supplying the nutrient solution of the present invention from day 5 after transfer to the soil;

(3) harvesting the seedlings grown for about 10 days after transfer to the topsoil;

(4) folding the collected seedlings;

(5) transferring the crushed seedlings and the remaining central stem seedlings to the top soil of (1) and cultivating them for 5 days without supplying nutrient solution;

(6) cultivating while supplying the nutrient solution of the present invention from day 5 after transfer to the soil; And

(7) harvesting the seedlings grown for 10-15 days after transfer to the top soil.

In the stem bark seedling production method described above, vermiculite is used as the top soil; Pearlite; sand; Mixed clay of perlite and vermiculite; Combination soil with sand and vermiculite; And it is possible to use preferably selected from the group consisting of a mixture of sand and peat moss, most preferably it is to use vermiculite.

Cultivation in the topsoil can be preferably carried out in an environment of temperature: about 24 ° C, work: about 16 hours, brightness: about 1500 lux, humidity: 50 to 60%.

The nutrient solution is transferred to the top soil and planted in the bottom irrigation from day 5. If the nutrient supply is made earlier than 5 days, the seedlings are decayed and annihilated.

After cultivating for about 10 days after planting on top soil, for example, the stem length (length) and diameter (diameter) reach an average of 9.1 cm and 2.0 mm (see Fig. 2c). It is preferable to carry out. In addition, stem cutting can produce a seedling having a good stem diameter of at least 95% of the stem diameter of the first seedling, and can be twisted up to about six times in this criterion. That is, in the production method, steps (3) to (6) may be repeated up to about six times.

In the production of stem pruning seedlings according to the present invention, the harvesting of the seedlings must be carried out by cutting the central stem using a surgical knife {see FIG. See FIG. 2B}. The use of scissors in the harvesting of seedlings is undesirable because it will rot into cut cotton.

Production method of stem pruning seedlings according to the present invention can be continuously doubled in about 10 days after transfer to the top soil can secure a large amount of seedlings in a short time, to reduce the production cost about 75% compared to tissue culture seedlings Can be.

In addition, the potato stem bark seedling provided by the present invention was increased 44.8 per week in the cultivation solution, 143 ~ 159% more than the cultivation of cultivation seedlings or artificial seed seed of the seed cultivation, 349 pieces per pyeong in culture medium Seedlings were increased by 142% compared with the culture medium nutrient solution, and the superiority was confirmed.

Tables 1 to 4 below show the results of checking the rooting rate and growth of stem stem seedlings.

Rooting Rate by Potato Stem Bark Seed Day 5 (Before Feeding) Vermiculite Pearlite sand Pearlite + Vermiculite Sand + Vermiculite Sand + Feet Moss 64.3% 46.4% 10.7% 35.7% 7.1% 0%

Root situation of potato stem cutting seedlings on the 10th, 15th day Culture Sato Day 10 Day 15 Root Count () Longest root length (cm) Root Count () Longest root length (cm) Nutrient Solution of the Invention Vermiculite Pearl Light Sand Pearl Light + Vermiculite Sand + Vermiculite Sand + Pit Moss 10.79.310.010.38.54.5 6.24.45.84.83.22.3 11.913.112.712.511.78.2 8.08.06.410.87.85.6 Average 8.9 4.5 11.7 7.8 Hyonex 1,000x Vermiculite Pearl Light Sand Pearl Light + Vermiculite Sand + Vermiculite Sand + Pit Moss 9.68.07.79.58.24.4 5.35.03.13.84.21.6 11.213.110.612.312.38.6 7.09.04.37.75.43.6 Average 7.9 3.8 11.4 6.2

Potato Stem Barrel Growth Day 10 Culture Sato Length (cm) Diameter (mm) Leaves () Leaf area (㎠) Live weight (mg / week) In building (mg / week) Nutrient solution of the present invention Vermiculite Pearl Light Sand Pearl Light + Vermiculite Sand + Vermiculite Sand + Pit Moss 9.79.19.39.08.78.9 2.02.11.92.12.12.0 4.14.04.24.34.23.9 15.718.09.615.011.711.0 569.3703.3671.3735.3640.0562.0 32.742.742.047.344.736.7 Average 9.1 2.0 4.1 13.5 646.9 41.0 Hyonex 1,000x Vermiculite Pearl Light Sand Pearl Light + Vermiculite Sand + Vermiculite Sand + Pit Moss 10.38.99.89.28.68.5 1.91.91.62.01.91.7 4.54.54.24.23.84.1 17.313.111.715.611.313.2 666.0578.0414.0675.3552.0433.3 40.740.727.344.737.330.0 Average 9.2 1.8 4.2 13.7 553.1 36.8

Second growth situation of 10 days after the 1st harvest Culture Sato Length (cm) Diameter (mm) Leaves () Nutrient Solution of the Invention Vermiculite Pearl Light Sand Pearl Light + Vermiculite Sand + Vermiculite Sand + Pit Moss 8.09.39.38.18.89.2 1.31.51.21.51.41.6 4.04.74.84.64.54.4 Average 8.8 1.4 4.5 Hyonex 1,000x Vermiculite Pearl Light Sand Pearl Light + Vermiculite Sand + Vermiculite Sand + Pit Moss 7.07.48.07.29.58.3 1.01.51.01.41.61.3 4.54.64.55.05.14.5 Average 7.8 1.3 4.7

As can be seen from the above results, the nutrient solution composition of the present invention has a good result in both rooting condition and above-ground growth situation compared to the Hyphenex 1000 fold solution, and it can be seen that the nutrient solution composition of the present invention is suitable as a potato stem cutting medium. .

Tables 5 and 6 below show the results of cultivating the potato stem bark seedlings provided by the present invention with a spraying mirror and a medium diameter nutrient solution, and are harvested at 70 days after the establishment.

Potato Spray Glasses Nutrient Cultivation Seedling types Tuber (dog / week) Tuber weight (g / week) % Distribution of tuber size Less than 1g 1-4g 5 ~ 14g 15-29 g More than 30g Stem 44.8 293.6 17.4 12.6 6.4 6.2 2.2 Tissue culture 31.4 213.8 10.4 7.0 7.2 6.6 0.2 Nutrient Cultivation Book 25.2 199.2 5.0 8.0 6.0 5.8 0.4 Artificial seed potatoes 28.2 101.8 15.6 6.0 4.2 2.0 0.4 Spray nutrient solution cultivation: Planting distance 30 × 20㎝

Potato culture solution for cultivation of nutrient solution Seedling types Total (pcs / 3.3㎡) Tuber size distribution (g) Average weight (g) Less than 5g 5 ~ 9g 10-19 g 20-29 g 30-49 g More than 50g Stem 349 154 102 52 25 10 6 9.5 Tissue culture 246 110 67 48 19 2 - 7.4 Culture medium culture nutrient solution: Planting distance 15 × 15㎝

As can be seen from the above results, potato stem bark seedlings provided by the present invention was increased to 44.8 per week in the nutrient solution cultivation results, 143 ~ 159% increase than the cultivation of cultivation seedlings or spray seed nutrient solution of artificial seed potatoes, At 349 pyeong, it was increased by 142% than when cultured cultured cultivation of tissue seedlings, the superiority was confirmed.

By supplying the nutrient solution of the specific composition provided by the present invention, it is possible to produce seedlings in the soil without contamination, and by applying the stem pruning method, the seedlings can be continuously doubled in about 10 days, thereby providing a large amount of seedlings in a short time. It can be secured, potato stem pruning seedling provided by the present invention can obtain a more than about 142% increase in the effect of cultivation cultivation seedlings or artificial seed potatoes.

Claims (8)

A nutrient solution composition for producing potato stem bark seedlings, the nutrient solution composition comprising the following composition: division Content (mg / l) Large amount of element KNO ₃ NH ₄ H ₂ PO ₄ Ca (NO ₃ ) ₂ 4H ₂ OMgSO ₄ 7H ₂ O 40578475250 Trace elements Fe-EDTAH ₃ BO ₃ MnSO ₄ 4H ₂ OZnSO ₄ 7H ₂ OCuSO ₄ 5H ₂ O (NH ₄ ) ₆ Mo ₇ O ₂₄ 20320.220.050.02 A method for producing potato stem bark seedlings, the production method comprising the following steps: (1) production by tissue culture and virus assaying, harvesting seedlings around 3 to 4 cm free of viruses and transferring them to the top soil for 5 days, supplying only water and cultivating them without supplying nutrient solution; (2) cultivating while supplying the nutrient solution according to claim 1 from day 5 after transfer to the soil; (3) harvesting the seedlings grown for about 10 days after transfer to the topsoil; (4) folding the collected seedlings; (5) transferring the crushed seedlings and the remaining central stem seedlings to the top soil of (1) and cultivating them for 5 days without supplying nutrient solution; (6) cultivating while supplying the nutrient solution of the present invention from day 5 after transfer to the soil; And (7) harvesting the seedlings grown for 10-15 days after transfer to the top soil. The method of claim 2, wherein the topsoil is vermiculite; Pearlite; sand; Mixed clay of perlite and vermiculite; Combination soil with sand and vermiculite; And a mixture of sand and pit moss. The method according to claim 2, wherein the cultivation on the top soil is carried out in an environment of temperature: about 24 ° C, length: about 16 hours, brightness: about 1500 lux, humidity: 50 to 60%. The method according to claim 2, wherein the supply of nutrient solution is carried out by bottom irrigation from the fifth day after planting in the soil. The method of claim 2, wherein steps (3) to (6) are repeated up to about six times. The method of claim 2, wherein the harvesting of the seedlings in the above steps (1), (3) and (7) is performed by cutting the central stem with a surgical knife. 3. Method according to claim 2, characterized in that the folding of the seedlings in step (4) is carried out using a knife or scissors.