CN107347608A - Virus-free seed potato and seedling water planting integration high power mating system - Google Patents
Virus-free seed potato and seedling water planting integration high power mating system Download PDFInfo
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- CN107347608A CN107347608A CN201710724029.8A CN201710724029A CN107347608A CN 107347608 A CN107347608 A CN 107347608A CN 201710724029 A CN201710724029 A CN 201710724029A CN 107347608 A CN107347608 A CN 107347608A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The present invention relates to virus-free seed potato and seedling water planting integration high power mating system, by the multiplication technique of water planting detoxic seedling and the integration of seedling potato seed water planting production technology, optimize nutrient solution ion concentration simultaneously, supplementary lighting sources light quality, hormone prescription and cutting seedlings number etc., the method stability and security of the present invention is good, operation and daily management are easy, safeguard low with production cost, and water planting potato plant seedling vigorous index can be significantly improved, improve the yield and quality of seedling and micro potato, expand plant breeding multiple, shorten the production cycle of micro potato, cultivation to seed potato and seedling is significant.
Description
Technical field
The invention belongs to cultivation technique without soil field, and in particular to virus-free seed potato and seedling water planting integration high power
Mating system.
Background technology
Potato is Solanaceae Solanum tuberous plant, is the important cereal crops in the whole world.But potato is planted for many years to be infected
The viral degeneration underproduction, average underproduction 30-50%.The major technique that whole world scientist solves to degenerate at present is by stem apex detoxification
Numerous seedling, then breeds micro potato and further breeds original seed and be used.Its virus-free seed potato mode of reproduction mainly has base
The modes such as matter cultivation, mist training, water planting and test tube cultivation.Wherein virus-free seed potato water planting raising technology, there is the numerous of seedling potato
Ability height is educated, operation sequence more simplifies.Tissue culture test tube seedling can be greatly reduced and breed quantity, save tissue culture floor space, there is provided
High quality seedling, micro potato breeding rate is greatly improved, improves micro potato quality.The technology can be at all potato detoxicating centers
Implement, there is the greatly market demand, have a extensive future.
Existing virus-free seed potato water planting production technology carries out numerous seedling and production of seed stock using " seedling potato point is numerous " mode,
I.e. early stage carries out cutting seedlings to test tube seedling using ciltivating process and cultivates rejuvenation, is passed into disuse after its female Miao Duoci cutting seedlings, causes to waste.
The strong sprout of cultivation is transplanted to the progress water planting potato seed of production seedbed and bred by the later stage, more transplantings links.Meanwhile though existing method
Water culture of potato production of seed stock needs are so disclosure satisfy that, but can not be that stolon, stem tuber occur when virus-free seed potato produces
The optimal growing environment with offer during expanding.Therefore it is unfavorable for stolon early to occur, mostly occur；It is unfavorable for potato tubers just
Often expand；Can not solve later stage tuber growth and root system nutrition absorbs the contradiction being co-located in nutrient solution；It can not reduce or subtract
The algae such as few green alga grow；Can not solve the too high too low problem of solution temperature.Therefore, there is an urgent need to from facility, nutrition
Liquid, environment, cutting seedlings technology and basic seedling variety introduction relation etc. carry out going deep into research and development, form a set of potato
The new method that virus-free seed potato and seedling water planting high power are bred.
The content of the invention
In view of this, it is an object of the invention to provide one kind in view of this, the present invention provides a kind of potato detoxicating kind
Potato and seedling water planting high power mating system, i.e., by detoxification test tube plantlet under the conditions of water planting continuous cutting seedlings take root expand numerous (transplanting), and
The growth of its female seedling stolon and stem tuber is induced by rational nutrient solution and light source afterwards, is realized " seedling potato is with numerous ".
To reach above-mentioned purpose, the present invention provides following technical scheme：
1st, virus-free seed potato and seedling water planting integration high power mating system, comprise the following steps：
1) by tissue cultural seedlings of free after hardening, be colonized in water planting breeding device take root, rejuvenation；
2) the 5cm stem sections grown in upper end are cut after seedling grows up to 10cm or so, remove after stem section radical leaves
50mg·L-120min is soaked in IBA solution, is then colonized in water planting breeding device；
3) by the Rooted Cuttings culture of field planting to 10~15cm of plant height, the thick 2~4mm of stem, tied during more than 5 big blades of band
Potato cultivates seedling；Condition of culture is：Solution temperature is 18 ± 2 DEG C, pH value 5.8~6.0；Environment temperature is controlled at 18~22 DEG C,
300 μm of ol m of intensity of illumination-2s-1, light application time 11h/ days；
4) after being colonized 20 days, the side shoot that Rooted Cuttings are grown is cut into downwards 5cm by top, cut after radical leaves in 50mgL- 120min is soaked in IBA solution, is then colonized in water planting breeding device, then cultivates seedling by step 3) culture for knot potato；
5) remove after being colonized 40 days and stalk is transferred into 2-3 section after plant base portion old leaf, nutrient solution is replaced by and ties the potato phase
Nutrient solution, it is more than 2g to potato wedge length to quality and obtains potato seed.
Preferably, step 1) is：Tissue cultural seedlings of free is taken off after film in 18~25 DEG C of lower refining seedlings 2-3 days, then by seedling from tissue culture
Remove old root along seedling base portion after being taken out in bottle and clean, by seedling lower end in 20mgL-1After soaking 20min in IBA solution, with treasure
In the water planting breeding device of water planting groove of the pearl cotton pipe parcel insertion containing clear water, change nutrient solution into after cultivating 1-2 days and tissue-cultured seedling is entered
Row is taken root, rejuvenation, and cultivating process regulation Depth Nutrient Solution is 2~4cm, and solution temperature is controlled at 25 ± 2 DEG C.
Preferably, also include after step 4), every 10 days, the side shoot that Rooted Cuttings are grown is cut into downwards 5cm by top, it is a collection of
Repetition is cut 2 times, is then cut after radical leaves in 50mgL-120min is soaked in IBA solution, is then colonized in water planting breeding device
In, then cultivate seedling by step 3) culture for knot potato.
It is preferred that step 3) in, field planting controlled the Depth Nutrient Solution to be 2-4cm before 20 days, and water planting breeding device is away from bottom level
5cm, nutrient solution circulation 5min, suspends 20min；After 20 days, adjustment cultivation bed bottom slope is 2~3 °, makes water inlet slightly higher
In delivery port, flowing liquid level keeps 1cm or so, nutrient solution circulation 3min, suspends 15min；Nutrient solution thoroughly changes one in every 30 days
It is secondary；Water planting breeding device bottom level is adjusted to 6~7cm after 40 days, nutrient solution is thoroughly changed once for every 20 days.
It is furthermore preferred that the nutrient solution each component concentration is as follows：KNO3 1900.00mg·L-1、NH4NO3
1410.00mg·L-1、KH2PO4 170.00mg·L-1、MgSO4·7H2O370.00mg·L-1、Ca(NO3)2·4H2O
705.00mg·L-1、H3BO3 6.20mg·L-1、MnSO4·4H2O 22.3mg·L-1、ZnSO4·7H2O 8.60mg·L-1、
Na2MoO4·2H2O 0.25mg·L-1、CuSO4·5H2O 0.025mg·L-1、CoCl2·6H2O 0.025mg·L-1、Na2·
EDTA 37.25mg·L-1、FeSO4·7H2O27.85mg·L-1。
It is furthermore preferred that the knot potato phase nutrient solution each component concentration is as follows：KNO3 1425.00mg·L-1、NH4NO3
1057.50mg·L-1、KH2PO4 127.50mg·L-1、MgSO4·7H2O 277.50mg·L-1、Ca(NO3)2·4H2O
528.75mg·L-1、H3BO3 6.20mg·L-1、MnSO4·4H2O 22.3mg·L-1、ZnSO4·7H2O 8.60mg·L-1、
Na2MoO4·2H2O 0.25mg·L-1、CuSO4·5H2O 0.025mg·L-1、CoCl2·6H2O 0.025mg·L-1、Na2·
EDTA 37.25mg·L-1、FeSO4·7H2O 27.85mg·L-1。
Preferably, in step 3), the illumination uses red blue light 5:1 LED is as light source.
Preferably, in step 4), 20mgL is sprayed within 10 days before each cutting seedlings-1GA and 0.5mgL-16-BA mixing swashs
Element.
The beneficial effects of the present invention are：Virus-free seed potato and seedling water planting integration high power mating system, with showing
There is technology to compare advantage specific as follows：
1) height, return duct open height, cultivation bed horizontal angle and the nutrient solution that cultivation plate is can adjust using solution culture facilities are followed
Ring time, seedling stage can ensure that seedling fully contacts with nutrient solution；The growth of root system, stolon and stem tuber can be increased after well developed root system
Space and the contact area with air, nutrient solution hold-up in groove is reduced, accelerate nutrient solution circulation, it is high greatly to alleviate the underground space
Wet hypoxemia and tuber growth space constraints；
2) cultivation plate field planting hole is designed as " convex " shape field planting hole and retainer plate, adds and is placed in internode number under dark surrounds
Amount, be advantageous to multiple stolon, solve cultivation plate folder potato, stop the problems such as crawling Stem nematode, and greatly simplify cultivation flow, carry
High system effectiveness；
3) increase screen pack, tuber growth is absorbed with root system nutrition and is spatially separating, reduce tuber growth ambient humidity, subtract
Few stem tuber hole skin quantity, improves stem tuber storage quality；
4) by optimizing solution temperature, nutrient solution ion concentration, light filling light quality, hormone and cutting the means such as branch to coordinate
The growth of above and below ground, seedling stage regulation and control improve cuttage seeding yield, and rear period regulation improves micro potato yield；
5) by nutrient solution and temperature control in greenhouse, the target uninterruptedly produced in the micro potato anniversary is realized；
6) liquid ultraviolet bactericidal lamp is installed and breeding device thickeies lucifuge and improved, water biography germ is solved and algae bio grows
The problem of；
7) cultivation system structure is simple, and cost and energy consumption are low, and administrative skill is less demanding, and seedling potato reproductive efficiency is high, can week
Year production, utilization rate of equipment and installations is high, and scale, factorial praluction can be achieved.
Therefore seed potato and seedling can be cultivated simultaneously using the method for the present invention, improve the production of seedling and micro potato
The quality of amount, expand plant breeding times, shorten the production period of micro potato；Operation sequence more simplifies simultaneously, is additionally favorable for reducing
Disease occurs during potato seed is stored, and improves potato seed quality, and the cultivation to seed potato and seedling is significant.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below and carried out
Explanation：
Fig. 1 is potato virus-free plantlet nutrient film hydroponic system sketch.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.
The solution culture facilities that the embodiment of the present invention uses are as follows：Water planting groove is formed by welded steel, and the wide high standard of flute length has
Two kinds of specification examinations of 370cm × 120cm × 20cm and 750cm × 120cm × 20cm, trench bottom and side laying water-proof sheet material, groove
Interior laying plastic leakage-proof film, cultivates the tilt adjustable section of trench bottom and horizontal plane, and cultivation slot structure is as shown in Figure 1.Wherein 1 is
Water planting production breeding device 1,2 is that liquid holding box body 2,3 is that liftable screen pack 3,4 is that nutrient solution supply pipe 4,5 is that delivery port 5,6 is
Waterproof ultraviolet lamp 6,7 is that temperature adjustment evaporator 7,8 is that float switch 8,9 is time controller water pump, and 10 be nutrition liquid pool,
21 be plastic sheeting, and 22 be cuboid tank tower, and 41 be nutrient solution supplying duct, outlet pipe 71, water inlet pipe 72, temperature inductor 73
With pure titanium evaporator 74.It is specifically shown in Publication No. CN205284507U Chinese patent.
Embodiment 1, virus-free seed potato and seedling water planting integration high power mating system
1) take off for examination tissue cultural seedlings of free after covering in room temperature (18~25 DEG C) lower refining seedling, take seedling from tissue culture bottle after 2-3 days
Remove old root after going out and clean, by seedling lower end in 20mgL-1Educated after 20min is soaked in IBA solution with the parcel insertion of pearl cotton pipe
In seedling device, clear water changes nutrient solution cultivation into water-cultured seedling bed after cultivating 1-2 days.Tissue-cultured seedling is taken root, rejuvenation, now adjusted
Return duct open height is saved, makes Depth Nutrient Solution in 2~4cm, base portion is fully contacted nutrient solution, solution temperature control exists
25±2℃；Shown in the following Tables 1 and 2 of nursery nutrient solution prescription, nutrient solution is prepared with deionized water entirely, and HNO is used after preparing3With
NaOH adjusts pH value 5.8~6.0, and nutrient solution exposed part covers thick cystosepiment, nutrient solution circulation 5min, suspends 30min.Nutrition
Liquid is thoroughly changed once for every 20 days.Nutrient solution ultraviolet germicidal is opened during nursery.
Table 1, nutrient solution and nursery nutrient solution prescription
Table 2, nutrient solution and nursery nutrition liquor trace element and molysite formula
Autumn and winter nursery need to carry out daily more than light filling 12h to Water culture seedling, and ensure warm indoor temperature at 25 DEG C, with
Promote cuttage root-taking and prevent seedling knot potato.
2) the 5cm stem sections grown in upper end are cut after seedling grows up to 10cm or so, remove after the big blade of stem section base portion
50mg·L-120min is soaked in IBA solution, stem section cuttage is entered into repeat step A in breeding device after being wrapped up with pearl cotton pipe, is repeated
Step B2 times；
3) the shearing seedling of rejuvenation is cultivated under the conditions of 25 ± 2 DEG C and can obtain within 10 days 10~15cm of plant height, stem thick 2~
4mm, the qualified knot potato cultivation seedling of more than the 5 big blade of band；Solution temperature is adjusted to 18 ± 2 DEG C, pH value 5.8~6.0；Environment
For temperature control at 18~22 DEG C, supplementary lighting sources selection light quality is red blue light 5:1 LED, intensity of illumination (photosynthetically active radiation,
PAR)300μmol m-2s-1, light application time 11h/ days, production period is interior to open ultraviolet germicidal lamp progress nutrient solution sterilization.
Red blue light 5:The advantages of 1 LED light quality, is protruded compared with conventional high-tension sodium vapor lamp energy saving, and light efficiency is excellent, promotes plant strain growth hair
Educate, plant strong sprout coefficient, leaf area, photosynthetic rate, improve into potato rate, micro potato yield etc..
Nutrient solution prescription is knot potato phase nutrient solution prescription, and as shown in table 3 below and table 4, nutrient solution ion is reduced after tying the potato phase
Concentration particularly N content to promote to tie potato, with deionized water prepared by all nutrient solutions.
Table 3, knot potato phase nutrient solution prescription
Table 4, knot potato phase nutrition liquor trace element and molysite formula
4) sprayed after being colonized 10 days every 10 days once by spraying GA 20mgL-1+6-BA 0.5mg·L-1Composition mixes
Hormone is closed, in triplicate；5cm is cut into as cuttage seeding in long branch top after 20 days, cuttage seeding is trained after removing the big blade of base portion
Support to cultivate seedling, cut once every 10 days afterwards, it is repeatable to cut 2~3 times and carry out numerous seedling；Not cutting seedlings coordinates again sprays 10mgL- 1GA or GA20mgL-1+6-BA 0.5mg·L-1Aqua is remarkably improved micro potato yield, under micro potato yield is notable after cutting seedlings
Drop；Cutting seedlings sprays GA 20mgL in 10 days before coordinating each cutting seedlings 3 times-1+6-BA 0.5mg·L-1Aqua, shear cuttage seeding number
Amount and overall seedling potato breeding rate highest.
The present embodiment is preferable, and field planting controlled Depth Nutrient Solution before 20 days be 2-4cm, and water planting breeding device is away from bottom level
5cm, nutrient solution circulation 5min, suspends 20min；After 20 days, adjustment cultivation bed bottom slope is 2~3 °, makes water inlet slightly higher
In delivery port, flowing liquid level keeps 1cm or so, nutrient solution circulation 3min, suspends 15min；Nutrient solution thoroughly changes one in every 30 days
It is secondary；Water planting breeding device bottom level is adjusted to 6~7cm after 40 days, nutrient solution is thoroughly changed once for every 20 days.
Remove in order to allow cutting seedlings to complete time relief normal plants, at 40 days and stalk is transferred 3 after plant base portion old leaf
Section, and nutrient solution is changed as knot potato formula, quality can continuously be plucked by, which starting within 60 days, is more than 2g qualified micro potato, but should reduce
Number is plucked, between reducing when under ground portion is shown in light.
In order to study influence of the different factors to growth and development of plants, photosynthetic rate and micro potato yield, Different Nutrition is used
Liquid ion concentration, different LED light fillings light qualities, the processing of GA and 6-BA hormotones, cultivation seedling branch cutting seedlings number processing, statistics are planted
Strain is grown, photosynthetic rate and final micro potato yield, as a result as shown in table 5~7.
The influence that table 5, regulation measure grow when being colonized 20 days to water planting potato plant
Note："-" represents that the data are not surveyed, and lowercase letter is different in nature in 5% horizontal significance difference, similarly hereinafter
The influence of table 6, regulation measure to water planting horse bell plant chlorophyll content and photosynthetic rate
Table 7, regulation measure finally shear the influence of cuttage seeding and micro potato reproductive effect to water planting Ma Ling
The cultivation management advantage of the present invention is mainly reflected in it can be seen from table 5~7：
1st, by carrying out dynamic regulation, vegetative growth phase ion concentration to water planting potato plant nutrient solution ion concentration
4248.51mg·L-1Nutrient solution prescription plant cauline leaf can be accelerated to grow, expand leaf area, chlorophyll content and photosynthetic rate,
Improve strong sprout coefficient (table 5,6).It is 3186.38mgL to tie potato phase ion concentration-1Nutrient solution prescription can remarkably promote micro potato
Formed, improve micro potato yield (table 7).
2nd, light quality 5:The LED light filling lower seedling stage of 1 red blue light can downgrade water planting plant, and increase leaf area, stem are thick and strong
Seedling coefficient, photosynthetic rate and chlorophyll content (table 5,6) are improved, micro potato unit area quantity has reached 1955 m-2, it is single
Position area amount has reached 5622gm-2, yield reached optimum level (table 7).
3rd, as known from Table 7, should be according to production mesh under the hormotone and cutting seedlings number Duplex treatment of GA, 6-BA composition
Reasonable selection cutting seedlings number is marked, the not cutting seedlings if based on being produced with micro potato, sprays GA20mgL-1+6-BA 0.5mg·L-1
Micro potato yield is remarkably improved, cutting seedlings will cause micro potato yield to be remarkably decreased.If pursuing maximum breeding rate, cutting seedlings 3
GA 20mgL are sprayed before the secondary each cutting seedlings of cooperation within 10 days-1+6-BA 0.5mg·L-1Aqua, shear cuttage seeding quantity and totality
Seedling potato breeding rate highest.
The method of the present invention is trained to the Yield comparison of micro potato with substrate culture and mist, as a result as represented shown in 8.
Table 8, seed potato and seedling water planting integration high power raising technology and substrate culture and mist training micro potato yield
Compare
Note：Single-plant propagation multiple=individual plant stem tuber number+individual plant shearing cuttage seeding number.Individual plant stem tuber number only refers to quality >=2g's
Micro potato quantity
As shown in Table 8, system water planting micro potato unit area quantity and yield of the present invention are all remarkably higher than substrate culture, and
The dosage of detoxic seedling can be greatly reduced compared with substrate culture；Compared with mist trains micro potato yield, unit area of the present invention is miniature
Potato quantity has met or exceeded the level of mist training.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (8)
1. virus-free seed potato and seedling water planting integration high power mating system, it is characterised in that comprise the following steps：
1) by tissue cultural seedlings of free after hardening, be colonized in water planting breeding device take root, rejuvenation；
2) the 5cm stem sections grown in upper end are cut after seedling grows up to 10cm or so, removed after stem section radical leaves in 50mgL-120min is soaked in IBA solution, is then colonized in the water planting breeding device of sky；
3) by the Rooted Cuttings culture of field planting to 10~15cm of plant height, the thick 2~4mm of stem, knot potato is obtained during more than 5 big blades of band and is planted
Train seedling；Condition of culture is：Solution temperature is 18 ± 2 DEG C, pH value 5.8~6.0；Environment temperature control is at 18~22 DEG C, illumination
300 μm of ol m of intensity-2s-1, light application time 11h/ days；
4) after being colonized 20 days, the side shoot that Rooted Cuttings are grown is cut into downwards 5cm by top, cut after radical leaves in 50mgL-1IBA
20min is soaked in solution, is then colonized in the water planting breeding device of sky, then cultivates seedling by step 3) culture for knot potato；
5) remove after being colonized 40 days and stalk is transferred into 2-3 section after plant base portion old leaf, nutrient solution is replaced by and ties the nutrition of potato phase
Liquid, it is more than 2g to potato wedge length to quality and obtains potato seed.
2. virus-free seed potato and seedling water planting integration high power mating system according to claim 1, it is characterised in that：
Step 1) is：Tissue cultural seedlings of free is taken off after film in 18~25 DEG C of lower refining seedlings 2-3 days, along seedling base after then seedling is taken out from tissue culture bottle
Portion removes old root and cleaned, by seedling lower end in 20mgL-1After soaking 20min in IBA solution, it is colonized in pearl cotton pipe parcel
In the breeding device of water planting groove containing clear water, cultivate 1-2 days after change into nutrient solution tissue-cultured seedling is taken root, rejuvenation, cultivated
Journey regulation Depth Nutrient Solution is 2~4cm, and solution temperature is controlled at 25 ± 2 DEG C.
3. virus-free seed potato and seedling water planting integration high power mating system according to claim 1, it is characterised in that：
Also include after step 4), every 10 days, the side shoot of Rooted Cuttings length is cut into downwards 5cm by top, a collection of repetition cuts 2 times, then
Cut after radical leaves in 50mgL-120min is soaked in IBA solution, is then colonized in water planting breeding device, then by step
3) culture cultivates seedling for knot potato.
4. virus-free seed potato and seedling water planting integration high power mating system according to claim 1, it is characterised in that：
In step 3), field planting controlled Depth Nutrient Solution before 20 days be 2-4cm, and field planting water planting breeding device follows away from bottom level 5cm, nutrient solution
Ring 5min, suspend 20min；After 20 days, adjustment cultivation bed bottom slope is 2~3 °, water inlet is higher than delivery port, flow fluid
Face keeps 1cm or so, nutrient solution circulation 3min, suspends 15min；Nutrient solution is thoroughly changed once for every 30 days；By water planting after 40 days
Breeding device bottom level is adjusted to 6~7cm, and nutrient solution is thoroughly changed once for every 20 days.
5. according to any one of Claims 1 to 4 virus-free seed potato and seedling water planting integration high power mating system, its
It is characterised by：The nutrient solution each component concentration is as follows：KNO3 1900.00mg·L-1、NH4NO3 1410.00mg·L-1、
KH2PO4170.00mg·L-1、MgSO4·7H2O370.00mg·L-1、Ca(NO3)2·4H2O 705.00mg·L-1、H3BO3
6.20mg·L-1、MnSO4·4H2O 22.3mg·L-1、ZnSO4·7H2O 8.60mg·L-1、Na2MoO4·2H2O
0.25mg·L-1、CuSO4·5H2O 0.025mg·L-1、CoCl2·6H2O 0.025mg·L-1、Na2·EDTA 37.25mg·
L-1、FeSO4·7H2O27.85mg·L-1。
6. according to any one of Claims 1 to 4 virus-free seed potato and seedling water planting integration high power mating system, its
It is characterised by：The knot potato phase nutrient solution each component concentration is as follows：KNO3 1425.00mg·L-1、NH4NO3 1057.50mg·
L-1、KH2PO4 127.50mg·L-1、MgSO4·7H2O 277.50mg·L-1、Ca(NO3)2·4H2O 528.75mg·L-1、
H3BO36.20mg·L-1、MnSO4·4H2O 22.3mg·L-1、ZnSO4·7H2O 8.60mg·L-1、Na2MoO4·2H2O
0.25mg·L-1、CuSO4·5H2O 0.025mg·L-1、CoCl2·6H2O 0.025mg·L-1、Na2·EDTA 37.25mg·
L-1、FeSO4·7H2O 27.85mg·L-1。
7. virus-free seed potato and seedling water planting integration high power mating system according to claim 1, it is characterised in that：
In step 3), the illumination uses red blue light 5:1 LED is as light source.
8. virus-free seed potato and seedling water planting integration high power mating system according to claim 1, it is characterised in that：
In step 4), 20mgL is sprayed within 10 days before each cutting seedlings-1GA and 0.5mgL-16-BA hormotones.
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| CN110367105A (en) * | 2019-08-22 | 2019-10-25 | 六盘水师范学院 | Root leek nutrient film water planting integrated production method |
| CN110574673A (en) * | 2019-09-29 | 2019-12-17 | 曾超凡 | Automatic water planting equipment of cuttage nursery stock |
| CN110612907A (en) * | 2019-11-12 | 2019-12-27 | 甘肃农业大学 | Potato seed culture system of detoxication of potato |
| CN111528023A (en) * | 2020-06-03 | 2020-08-14 | 昭通市农业科学院 | Potato seedling planting method |
| CN113728920A (en) * | 2021-08-03 | 2021-12-03 | 福建省亚热带植物研究所 | Device for plant detoxification, seedling transplanting and rooting culture |
| CN114467751A (en) * | 2022-02-17 | 2022-05-13 | 延边大学 | Device for producing detoxified potato mini-potatoes in vermiculite matrix |
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| CN114747475A (en) * | 2022-06-06 | 2022-07-15 | 湖南省棉花科学研究所 | Water culture device and method for sweet potato seedling propagation |
| CN114982617A (en) * | 2022-06-27 | 2022-09-02 | 武汉兰多生物科技有限公司 | Water culture seedling hardening method for improving transplanting survival rate of Arundo donax tissue culture seedlings |
| CN116114583A (en) * | 2022-11-18 | 2023-05-16 | 定西马铃薯研究所(普通合伙) | Light simplified open breeding method for potato virus-free seedlings |
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Application publication date: 20171117 |