CN106508644A - Sweet potato virus-free breeding method - Google Patents
Sweet potato virus-free breeding method Download PDFInfo
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- CN106508644A CN106508644A CN201610963786.6A CN201610963786A CN106508644A CN 106508644 A CN106508644 A CN 106508644A CN 201610963786 A CN201610963786 A CN 201610963786A CN 106508644 A CN106508644 A CN 106508644A
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- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The invention discloses a sweet potato virus-free breeding method, which comprises the original-original spice seedling breeding, particularly comprises steps of washing a virus-free bottle sprout and adding a medium, then colonizing the sprout on a water planting colonization plate; covering a transparent water-retaining film; and culturing the sprout with tap water; adding 1/2 of a first nutrient solution for culturing continuously when the sprout grows new white root; cutting off the shoot tip at the upper portion of the seedling when the seedling grows long for original-original specie production; supplementing 1/2 of the first nutrient solution, and cutting off the shoot tip at the upper portion of the lateral bud when the lateral bud grows long for original-original spice production. <{EN2}>According to the method, the tissue culture plants are bred by water planting first, and the survival rate can be up to 100%; the sprouts can be cut once every 7 days; the sprouts can be cut by 5-6 times in one colonization; 1 sprout can breed about 20 sprouts within two months; the factory production of the virus-free sprout can be realized completely to realize vigorous seedling and rapid propagation.
Description
Technical field
The invention belongs to crop breeding field, is related to the propagation method of potato seed.
Background technology
Rhizoma Dioscoreae esculentae (I pomoeas batatas Lam.) it is the important grain of China, feedstuff and insutrial crop, have
The advantages of nutritious, yield stability is good, yield is high, plays an important role in social economy.China's Rhizoma Dioscoreae esculentae cultivated area is occupied
First place in the world, total output account for more than the 80% of global total output.Rhizoma Dioscoreae esculentae is mainly bred by vines, potato wedges and rice shoot, production
Middle susceptible viral infects, the symptom such as cause leaf-shrinkage, plant short and small, causes quality to deteriorate, and yield is reduced year by year.Rhizoma Dioscoreae esculentae field
Plant viral infection rate causes the production loss of 25%-57%, China's year to put down in 30%-60%, serious reachable 80%-90% every year
Up to 29.4%, year loss is up to more than 4,000,000,000 yuan for the underproduction.
Key component of the Rhizoma Dioscoreae esculentae as modern agriculture, in people's lives, shared proportion is increasing.But peasant
, mostly based on reserving seed for planting certainly, kind sexual involution is increasingly severe for sweet potato planting, in particular with spreading for NEW TYPE OF COMPOSITE virus SPVD,
Caused yield reduction, the quality decline etc. of degenerating becomes clear day by day, it is necessary to carries out rejuvenation to its kind of property by detoxification, could recover
Its high yield and high-quality.
In prior art, the cultivation of general detoxic seedling and application program are as follows：
1. regeneration plant is obtained with Shoot Tip Culture,
2. Jing Viral diagnosis determine the detoxic seedling of Virusfree,
3. original silkworm egg potato is produced using detoxic seedling,
4. next year original silkworm egg potato accelerating germination obtains original seed seedling,
5. original seed seedling plant land for growing field crops breeding stock ground obtains original seed potato seed,
6. original seed potato seed can be planted as using for production commodity potato, i.e. the sprout plant land for growing field crops of original seed potato seed accelerating germination, the Rhizoma Dioscoreae esculentae of production
Potato wedges can be used as commodity potato；Also potato seed use is may continue as, but infects the probability of virus increasing, cause under yield and quality
Drop.
In process of the present invention is realized, inventor has found that at least there are the following problems in prior art：
1st, roc straight cutting breeding coefficient is very low, and survival rate is low.Tissue cultured seedling is directly planted on net canopy soil, because its Seedling is little, grown
Gesture is weak, cuts within 15-20 days once, and survival rate is 80% or so.
2nd, in soil, knot potato is slow and seed tuber size is uneven for detoxic seedling cuttage.
Term is explained：
The potato seed planted out in disease-free net canopy with original silkworm egg Seedling, as original silkworm egg.The Seedling brought out using original silkworm egg, Jing it is fast it is numerous after
Plant the potato seed produced in the disease-free seed field of summer potato, as original seed.
The content of the invention
In consideration of it, present invention aim at providing a kind of quick, efficiently breeding Sweetpotato Viruses Elimination potato seed method.
Inventor is by long-term exploration and trial, and multiple experiment and effort, constantly reforms and innovates, is solution
Above technical problem, the technical scheme that the present invention is provided are to provide a kind of Sweetpotato Viruses Elimination potato seed propagation method, including original silkworm egg kind
Seedling breeds step, specific as follows：
a）Water planting field planting plate is colonized in after detoxification bottle seedling is cleaned culture medium, transparent water-retaining film is covered, is trained with tap water
Support；
b）Treat that seedling grows 0.4-0.6cm white new roots, 1/2 first nutritional solution of addition continues culture；
c）Seedling length is treated to 15-20cm, seedling top 9-11cm stem apexs are cut, is produced for original silkworm egg；
d）1/2 first nutritional solution is supplemented, and lateral bud length is treated to 15-20cm, is cut lateral bud top 9-11cm stem apexs, for original silkworm egg
Production；Repeat this step 4-5 time.
According to an embodiment of Sweetpotato Viruses Elimination potato seed propagation method of the present invention, also including original silkworm egg production stage, tool
Body is as follows：
e）Careless carbon-coating is laid in net canopy；By the shoot tip plant on turf, bottom water is poured；
f）Pour and apply the second nutritional solution, promote expanding for feeding root, by beating the prosperous length of climing control stem and leaf in growth course；
g）Harvesting potato kind, as original silkworm egg.
According to a preferred implementation of Sweetpotato Viruses Elimination potato seed propagation method of the present invention, on the field planting plate, array is arranged
There is field planting hole, aperture is 0.5cm, and pitch of holes is 5*5cm.
According to a specific embodiment of Sweetpotato Viruses Elimination potato seed propagation method of the present invention, the transparent water-retaining film is transparent
Plastic sheeting.
According to a further embodiment of Sweetpotato Viruses Elimination potato seed propagation method of the present invention, first nutritional solution is
Following compound is dissolved in water to make：NH4NO3、KNO3、Ca(NO3)2、KH2PO4、MgSO4.7H2O。
According to one of Sweetpotato Viruses Elimination potato seed propagation method of the present invention preferred embodiment, it is each in first nutritional solution
Compound and its content are：1268g/kg NH4NO3、1795g/kg KNO3、492g/kg Ca(NO3)2、170g/kg KH2PO4、
370g/kg MgSO4.7H2O。
According to one of Sweetpotato Viruses Elimination potato seed propagation method of the present invention preferred embodiment, the careless carbon layers having thicknesses are
20-25cm；The density of cuttage is 10*15cm.
According to a further embodiment of Sweetpotato Viruses Elimination potato seed propagation method of the present invention, second nutritional solution is
Following compound is dissolved in water to make：NH4NO3、KNO3、CaCl2·H2O、KH2PO4、MgSO4.7H2O。
According to one of Sweetpotato Viruses Elimination potato seed propagation method of the present invention preferred embodiment, it is each in second nutritional solution
Compound and its content are：1650g/kg NH4NO3、1389g/kg KNO3、440g/kg CaCl2·H2O、170g/kg
KH2PO4、370g/kg MgSO4.7H2O。
According to a preferred embodiment of Sweetpotato Viruses Elimination potato seed propagation method of the present invention, the concrete steps of the method are such as
Under：
a）Water planting field planting plate is colonized in after detoxification bottle seedling is cleaned culture medium, transparent water-retaining film is covered, is cultivated with tap water
5-7 days；
b）Treat that seedling grows 0.4-0.6cm white new roots, 1/2 first nutritional solution of addition continues culture；First nutritional solution
In, the ratio of the amount of the material of N, P, K is 55.47:1.25:19.02, NH4 +/NO3 -Material amount ratio be 0.40；
c）After culture 7-10 days, seedling length cuts seedling top 9-11cm stem apexs to 15-20cm, produces for original silkworm egg；
d）1/2 first nutritional solution is supplemented, and is continued culture 7-10 days, lateral bud length is treated to 15-20cm, cut lateral bud top 9-11cm
Stem apex, produces for original silkworm egg；Repeat this step 4-5 time；
e）The thick careless carbon-coatings of 20-25cm are laid in net canopy；By step c）With step d）In the shoot tip plant cut in turf
On, cutting density is 10*15cm, pours bottom water；
f）Pour and apply the second nutritional solution, promote expanding for feeding root, by beating the prosperous length of climing control stem and leaf in growth course；Described
In two nutritional solutions, the ratio of the amount of the material of N, P, K is 55.00:1.25:15.00, NH4 +/NO3 -The ratio of amount of material be
0.60；
g）Harvesting potato kind, as original silkworm egg.
Compared with prior art, a technical scheme in above-mentioned technical proposal has the advantage that：
A) tissue cultured seedling is first passed through water planting and is bred by the inventive method, survival rate can reach 100%, and 7 days can cutting seedlings once,
Once field planting can be with cutting seedlings 5-6 time, and 1 Seedling can breed out 20 Seedlings or so in two months, fully achieve the factorial praluction of detoxic seedling,
Reach strong sprout and fast numerous purpose.
B) original silkworm egg mating system of the present invention, results original silkworm egg, potato wedges size 50-100 gram by 30 days or so, individual plant can
3-6 original silkworm egg, cutting seedling knot potato morning are harvested, and it is uniform in size to tie potato.
C) Rhizoma Dioscoreae esculentae original silkworm egg seeling industry can only be carried out on super-clean bench indoors under routine, it is desirable to higher, and grow compared with
Slowly, 20 days or so 1 Seedlings can only breed 4-5 Seedlings, and Seedling is weak, be difficult to survive.The improved original silkworm egg Seedling tradition side of breeding of the present invention
Formula, low cost, instant effect, the original silkworm egg Seedling bred out are sturdy, easily survive for numerous Seedling again or original silkworm egg production.
D) cuttage of original silkworm egg Seedling is carried out into original silkworm egg production in soil under usual manner, the time is long, it usually needs 150 days very
To longer, slowly effect, inefficiency is bred.Rhizoma Dioscoreae esculentae original silkworm egg of the present invention is bred using Soilless Culture Methods, from plantation to
Results only need 30-50 days, and what is greatly improved breeds efficiency, the popularization, the update of improved seeds, solution to quickening new varieties
The certainly dip-dye of SPVD and similar virus provides effective approach, accelerates the process of Sweet Potato Industry development.
Specific embodiment
Illustrate with reference to specific embodiment.
To make purpose, technical scheme and the advantage of embodiment of the present invention clearer, below in conjunction with present invention enforcement
Mode, is clearly and completely described to the technical scheme in embodiment of the present invention, it is clear that described embodiment is
The a part of embodiment of the present invention, rather than the embodiment of whole.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment obtained under the premise of creative work is not made by art personnel, belongs to the model of present invention protection
Enclose.
It is in prior art, as follows the step of the breeding method of general detoxic seedling：
Regeneration plant is obtained with Shoot Tip Culture, Jing Viral diagnosis determine the detoxic seedling of Virusfree, it is former using detoxic seedling production
Potato seed, next year original silkworm egg potato accelerating germination obtain original seed seedling, and original seed seedling plant land for growing field crops breeding stock ground obtains original seed potato seed.Original seed potato seed
Can plant as using for production commodity potato, i.e. the sprout plant land for growing field crops of original seed potato seed accelerating germination, the Rhizoma Dioscoreae esculentae potato wedges of production can be used as commodity
Potato；Also potato seed use is may continue as, but infects the probability of virus increasing, cause yield and quality to decline.
The present invention is improved to the method that the mode of reproduction and original silkworm egg of Rhizoma Dioscoreae esculentae original silkworm egg seedling are produced, inventor
In addition to being improved to the mode that tradition is bred, also the nutritional solution without soil breeding is improved, is promoted original silkworm egg kind
The health of Seedling and original silkworm egg is bred.
Sweetpotato Viruses Elimination potato seed propagation method of the present invention, including two key steps.First step is that original silkworm egg seedling is numerous
Step is educated, second step is original silkworm egg production stage.Under the support of other prior arts, the two steps can be independent
Implement.For example, the step of those skilled in the art can individually implement original silkworm egg seedling breeding, and original silkworm egg is adopted the step of production
It is replaced with prior art.
Sweetpotato Viruses Elimination potato seed propagation method described by the present embodiment, by above-mentioned two step, i.e. original silkworm egg seedling breeding
Step and original silkworm egg production stage are combined together and are described, and comprise the following steps that.Wherein, step a）To d）For original silkworm egg kind
Seedling breeds step, step e）-g）For original silkworm egg production stage.
a）Water planting field planting plate is colonized in after detoxification bottle seedling is cleaned culture medium, transparent water-retaining film is covered, is carried out with tap water
Culture 5-7 days.The a diameter of 0.5cm of field planting hole on field planting plate, pitch of holes is 5*5cm.Field planting hole aperture and pitch-row on field planting plate
Size Selection be preferably selecting and recommending for the present invention, certain those skilled in the art can be carried out without creation on this basis
The expansion of property.
b）After originally Aquaponic 5-7 days, more than 70% seedling grows 0.5cm white new roots, adds 1/2 first nutrition
Liquid continues culture；In first nutritional solution, the ratio of the amount of the material of N, P, K is 55.47:1.25:19.02, NH4 +/NO3 -'s
The ratio of the amount of material is 0.40.First nutrient solution prescription is as shown in table 1.
1 first nutrient solution prescription of table
c）After culture 7-10 days, seedling length cuts seedling top 9-11cm stem apexs to 15-20cm, produces for original silkworm egg.
d）1/2 first nutritional solution is supplemented, and is continued culture 7-10 days, lateral bud length is treated to 15-20cm, cut lateral bud top 9-
11cm stem apexs, produce for original silkworm egg；Repeat this step 4-5 time.It is object into potato 007 to the west of inventor, has carried out contrast real
Test, correction data is shown in Table 2.
2 west of table is contrasted into the different numerous Seedling modes of 007 tissue cultured seedling of potato
From table 2 it can be seen that the present invention is compared with the conventional method, survival rate reaches 100%, to the limit.The cutting seedlings cycle is big
Width shortens, and can increase cutting seedlings number of times, further lift numerous Seedling total amount in same time.The present invention is once colonized and can cut altogether
Seedling 5-6 time, 1 plant of planting stock can breed out 20 Seedlings or so in two months, fully achieve the factorial praluction of detoxic seedling.
e）The thick careless carbon-coatings of 20-25cm are laid in net canopy；By step c）With step d）In the shoot tip plant cut in grass
On charcoal, cutting density is 10*15cm, pours bottom water.The present embodiment Rhizoma Dioscoreae esculentae original silkworm egg Soilless Culture Methods are bred, and potato wedges is big
Little uniform, harvesting potato seed is convenient, quick, it is to avoid potato seed surface damage, it is to avoid broken potato, rotten potato.Using turf, favorably
In ensureing that the fast robust growth of potato, potato skin are smooth, without lateral root.
Turf, is the product in formation process of bog, is formed at the Quaternary Period, by the residuum of limnocryptophyte, in the suspicion of many water
Under the conditions of gas, it is impossible to decompose accumulation completely and form.The plant residue that thoroughly do not decomposed containing large quantity of moisture and, humus with
And a part of mineral.More than 30%, quality is soft to be easy to fragmentary to the content of organic matter, and proportion 0.7-1.05 is more in brown or black
Color, with flammable and inspiratory.
f）Cuttage is poured after 15 days and applies the second nutritional solution, is promoted expanding for feeding root, is applied weekly once, is led in growth course
Cross and beat the prosperous length of climing control stem and leaf.In second nutritional solution, the ratio of the amount of the material of N, P, K is 55.00:1.25:15.00, NH4 +/NO3 -Material amount ratio be 0.60.The formula of the second nutritional solution is shown in Table 3.
3 second nutrient solution prescription of table
Can harvest within 50 days or so, potato wedges size 50-100 gram, individual plant can harvest 3-6 original silkworm egg.
g）Harvesting potato kind, as original silkworm egg.
Nontoxic original silkworm egg robust growth that the inventive method is obtained, no disease and pests harm, potato skin be smooth, without lateral root, Virus-free Sweetpotato
With germinating good, land for growing field crops growing way it is strong, knot potato is early, potato wedges expand soon, knot potato concentrate, neatly, potato skin color is vivid, commodity
Well, the advantages of disease resistance is strong, volume increase the effect of protective yield are notable.
Rhizoma Dioscoreae esculentae original silkworm egg seeling industry can only be carried out on super-clean bench indoors under routine, it is desirable to higher, and growth is relatively delayed
Slowly, 20 days or so 1 Seedlings can only breed 4-5 Seedlings, and Seedling is weak, be difficult to survive.The improved original silkworm egg Seedling of the present invention is bred, low cost,
Instant effect, the original silkworm egg Seedling bred out are sturdy, easily survive for numerous Seedling again or original silkworm egg production.
The cuttage of original silkworm egg Seedling is carried out into original silkworm egg production in soil under usual manner, the time is long, it usually needs 150 days even
Longer, slowly effect breeds inefficiency.Rhizoma Dioscoreae esculentae original silkworm egg Soilless Culture Methods of the present invention are bred, and only harvest from planting to
50 days or so are needed, what is greatly improved breeds efficiency, the popularization, the update of improved seeds, solution to quickening new varieties
The dip-dye of SPVD and similar virus provides effective approach, accelerates the process of Sweet Potato Industry development.
The above is only the preferred embodiment of the present invention, it is noted that it is right that above-mentioned preferred implementation is not construed as
The restriction of the present invention, protection scope of the present invention should be defined by claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made, these change
Enter and retouch also to should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of Sweetpotato Viruses Elimination potato seed propagation method, it is characterised in that including original silkworm egg seedling breeding step, specific as follows：
Water planting field planting plate is colonized in after detoxification bottle seedling is cleaned culture medium, transparent water-retaining film is covered, is cultivated with tap water；
Treat that seedling grows 0.4-0.6cm white new roots, 1/2 first nutritional solution of addition continues culture；
Seedling length is treated to 15-20cm, seedling top 9-11cm stem apexs are cut, is produced for original silkworm egg；
1/2 first nutritional solution is supplemented, lateral bud length is treated to 15-20cm, is cut lateral bud top 9-11cm stem apexs, give birth to for original silkworm egg
Produce；Repeat this step 4-5 time.
2. Sweetpotato Viruses Elimination potato seed propagation method according to claim 1, it is characterised in that also walk including original silkworm egg production
Suddenly, it is specific as follows：
Careless carbon-coating is laid in net canopy；By the shoot tip plant on turf, bottom water is poured；
Pour and apply the second nutritional solution, promote expanding for feeding root, by beating the prosperous length of climing control stem and leaf in growth course；
Harvesting potato kind, as original silkworm egg.
3. Sweetpotato Viruses Elimination potato seed propagation method according to claim 1, it is characterised in that array is arranged on the field planting plate
There is field planting hole, aperture is 0.5cm, and pitch of holes is 5*5cm.
4. Sweetpotato Viruses Elimination potato seed propagation method according to claim 1, it is characterised in that the transparent water-retaining film is transparent
Plastic sheeting.
5. Sweetpotato Viruses Elimination potato seed propagation method according to claim 1, it is characterised in that first nutritional solution is in water
In dissolve in following compound and make：NH4NO3、KNO3、Ca(NO3)2、KH2PO4、MgSO4.7H2O。
6. Sweetpotato Viruses Elimination potato seed propagation method according to claim 6, it is characterised in that respectively change in first nutritional solution
Compound and its content are：1268g/kg NH4NO3、1795g/kg KNO3、492g/kg Ca(NO3)2、170g/kg KH2PO4、
370g/kg MgSO4.7H2O。
7. Sweetpotato Viruses Elimination potato seed propagation method according to claim 2, it is characterised in that the careless carbon layers having thicknesses are 20-
25cm；The density of cuttage is 10*15cm.
8. Sweetpotato Viruses Elimination potato seed propagation method according to claim 2, it is characterised in that second nutritional solution is in water
In dissolve in following compound and make：NH4NO3、KNO3、CaCl2·H2O、KH2PO4、MgSO4.7H2O。
9. Sweetpotato Viruses Elimination potato seed propagation method according to claim 2, it is characterised in that respectively change in second nutritional solution
Compound and its content are：1650g/kg NH4NO3、1389g/kg KNO3、440g/kg CaCl2·H2O、170g/kg KH2PO4、
370g/kg MgSO4.7H2O。
10. Sweetpotato Viruses Elimination potato seed propagation method according to claim 2, it is characterised in that the concrete steps of the method are such as
Under：
a）Water planting field planting plate is colonized in after detoxification bottle seedling is cleaned culture medium, transparent water-retaining film is covered, is cultivated with tap water
5-7 days；
b）Treat that seedling grows 0.4-0.6cm white new roots, 1/2 first nutritional solution of addition continues culture；First nutritional solution
In, the ratio of the amount of the material of N, P, K is 55.47:1.25:19.02, NH4 +/NO3 -Material amount ratio be 0.40；
c）After culture 7-10 days, seedling length cuts seedling top 9-11cm stem apexs to 15-20cm, produces for original silkworm egg；
d）1/2 first nutritional solution is supplemented, and is continued culture 7-10 days, lateral bud length is treated to 15-20cm, cut lateral bud top 9-11cm
Stem apex, produces for original silkworm egg；Repeat this step 4-5 time；
e）The thick careless carbon-coatings of 20-25cm are laid in net canopy；By step c）With step d）In the shoot tip plant cut in turf
On, cutting density is 10*15cm, pours bottom water；
f）Pour and apply the second nutritional solution, promote expanding for feeding root, by beating the prosperous length of climing control stem and leaf in growth course；Described
In one nutritional solution, the ratio of the amount of the material of N, P, K is 55.00:1.25:15.00, NH4 +/NO3 -The ratio of amount of material be
0.60；
g）Harvesting potato kind, as original silkworm egg.
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Cited By (8)
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| CN107347638A (en) * | 2017-06-27 | 2017-11-17 | 福建百卉花艺有限公司 | A kind of ciltivating process of succulent |
| CN107347608A (en) * | 2017-08-22 | 2017-11-17 | 西南大学 | Virus-free seed potato and seedling water planting integration high power mating system |
| CN107593302A (en) * | 2017-10-10 | 2018-01-19 | 平顶山市农业科学院 | A kind of method accelerated New variety of leek and cultivate process |
| CN109329027A (en) * | 2018-12-03 | 2019-02-15 | 湖南省作物研究所 | A kind of efficient propagation method of sweet potato |
| CN109757231A (en) * | 2018-12-24 | 2019-05-17 | 江苏省农业科学院 | A kind of method that Chinese yam idioplasm resource is quickly bred and saved |
| CN110622863A (en) * | 2019-10-18 | 2019-12-31 | 福建农林大学 | Method for improving transplanting survival rate of sweet potato virus-free tissue culture seedlings |
| WO2021240354A1 (en) * | 2020-05-26 | 2021-12-02 | Pieters Joseph & Luc Bv | Propagating material for sweet potato |
| CN114051927A (en) * | 2021-11-09 | 2022-02-18 | 延安市农业科学研究所 | Simplified breeding method for virus-free sweet potato seedlings |
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