CN116210590A - Potato plant tissue culture seedling cutting propagation transfer device and transfer method thereof - Google Patents
Potato plant tissue culture seedling cutting propagation transfer device and transfer method thereof Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000012546 transfer Methods 0.000 title claims abstract description 30
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 28
- 238000005520 cutting process Methods 0.000 title claims abstract description 25
- 238000004161 plant tissue culture Methods 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 136
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention discloses a potato plant tissue culture seedling cutting propagation transfer device and a transfer method thereof, wherein the transfer device comprises a movable frame; the bottom of the movable frame is provided with a travelling mechanism; the movable frame is provided with a water tank; the water tank is internally divided into a clear water cavity, a medicament cavity and a waste water cavity by a partition plate group; the movable frame is also provided with a cleaning soaking tank; the cleaning soaking tank is connected with the water tank; tissue culture seedlings are placed in the cleaning soaking tank at intervals; the cleaning soaking tank is used for cleaning tissue culture seedling roots when clear water flows in the cleaning soaking tank; the cleaning soaking tank is used for soaking the tissue culture seedling roots when the cleaning soaking tank contains a medicament; the bottom of the cleaning soaking tank is connected with a drain pipe; the drain pipe is provided with a timing water pump. The cleaning process and the soaking process can be automatically completed in the moving process of the moving frame, and operators only need to operate seedling placement, seedling taking and seedling planting, so that the time for tissue culture and breeding is greatly shortened.
Description
Technical Field
The invention relates to the technical field of potato crop breeding and cultivation, in particular to a potato plant tissue culture seedling cutting and propagation transfer device and a transfer method thereof.
Background
Sweet potatoes (Ipomoea batatas) are also called sweet potatoes, sweet potatoes and sweet potatoes, are annual or perennial herbs of the genus sweet potatoes of the family Convolvulaceae, are rich in proteins, starch, pectin, cellulose and various minerals, are important crops for grains, feeds and industrial raw materials, and are arranged in the seventh position in total production in the world grain production. The sweet potato is drought-resistant, barren-resistant, high in biological yield, and is one of important renewable energy raw materials except eating, the current prevention and treatment of the sweet potato virus disease generally adopts stem tip meristems to be stripped, and virus-free seedlings are obtained after in vitro culture to remove viruses, and the virus-free seed potato is cultivated, propagated and applied. The sweet potato seedlings after virus removal recover the seed character, the growth vigor is enhanced, the yield is improved, the quality is changed, the yield increasing effect is obvious, the yield increasing amplitude can reach more than 80 percent, and the method has better application prospect in production.
The sweet potato detoxified seedling can grow into a complete plant after 30 days on a proper rapid propagation culture medium, and then seedling hardening and transplanting can be carried out. During transplanting, the tissue culture seedlings are taken out of the seedling hardening culture flask by forceps, residual agar at the root is washed off by clean water, soaked for 10-15min by formaldehyde solution, and then transplanted onto a culture medium consisting of vermiculite, perlite, coarse sand, furnace ash, chaff, sawdust and the like. Because the tissue culture seedlings are easy to damage, the existing operation mode is that the tissue culture seedlings are manually taken out and then washed by clean water, then the washed tissue culture seedlings are soaked in formaldehyde solution, and the tissue culture seedlings are placed in a cart to be moved into a greenhouse for transplanting after the soaking is finished; such an operation has the following problems: when the number of the tissue culture seedlings is too large, a great deal of time and labor are wasted when the tissue culture seedlings are washed by running water in sequence, and the tissue culture seedlings are easy to wash and not thorough; in addition, after the tissue culture seedlings are soaked in formaldehyde solution, the tissue culture seedlings need to be completely taken out and separated from the formaldehyde solution in time, otherwise, the soaking time is too long, so that the cells are easy to lose water to cause root necrosis; the tissue culture seedlings after the soaking are cultivated to the culture bed in sequence by manpower, and the process needs a certain time, and the survival rate of the tissue culture seedlings is affected due to the fact that the tissue culture seedlings are easy to damage due to improper management and storage in the process of waiting for cultivation.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a potato plant tissue culture seedling cutting propagation transfer device and a transfer method thereof.
The aim of the invention is achieved by the following technical scheme: a potato plant tissue culture seedling cutting propagation transfer device comprises a movable frame; the bottom of the movable frame is provided with a travelling mechanism; the movable frame is provided with a water tank; the water tank is internally divided into a clear water cavity, a medicament cavity and a waste water cavity by a partition plate group; the movable frame is also provided with a cleaning soaking tank; the cleaning soaking tank is connected with the water tank; tissue culture seedlings are placed in the cleaning soaking tank at intervals; the cleaning soaking tank is used for cleaning tissue culture seedling roots when clear water flows in the cleaning soaking tank; the cleaning soaking tank is used for soaking the tissue culture seedling roots when the cleaning soaking tank contains a medicament; the bottom of the cleaning soaking tank is connected with a drain pipe; the drain pipe is provided with a timing water pump;
further, one end of the bottom of the cleaning soaking tank is provided with a water inlet pipe; the drain pipe is arranged at the other end of the bottom of the cleaning soaking tank; a placing disc is movably arranged in the cleaning soaking tank; seedling inserting holes are formed in the placing plate at intervals;
further, a filter screen is arranged between the placing plate and the bottom of the cleaning soaking tank;
further, a groove is formed in one end face of the cleaning soaking tank; the groove is hinged with a sealing door; the sealing door is provided with a waterproof rubber ring;
further, sliding rails are arranged on two side walls of the cleaning soaking tank at intervals; the two sides of the placing plate are provided with supporting rails corresponding to the sliding rails;
further, a plurality of layers of cleaning soaking tanks are arranged on the movable frame at intervals; the water inlet pipe is connected to the clean water cavity through a main water pipe; the drain pipe is connected to the waste water cavity through a main drain pipe; the main water pipe is connected with a medicament pipe through a tee joint; the medicament tube is connected to the medicament lumen;
further, a first electromagnetic valve is arranged on the water inlet pipe; the drain pipe is provided with a second electromagnetic valve; a first liquid pump is arranged on the main water pipe; a second liquid pump is arranged on the medicament tube;
the potato plant tissue culture seedling cutting propagation transfer method adopts the potato plant tissue culture seedling cutting propagation transfer device;
the further technical proposal is that the method comprises the following steps:
1) The tissue culture seedlings are inserted into the seedling inserting holes on the placing tray, and the placing tray is placed into the cleaning soaking tank;
2) When the movable frame moves to the transplanting greenhouse, a first liquid pump on the main water pipe, a first electromagnetic valve on the water inlet pipe, a second electromagnetic valve on the drain pipe and a timing water pump are opened, clear water is pumped out from a clear water cavity in the water tank, the clear water flows in the cleaning soaking tank, and agar remained at the root of the tissue culture seedling is cleaned;
3) After the cleaning is finished, the first liquid pump, the second electromagnetic valve and the timing water pump are closed; a second liquid pump on the medicament tube is started, formaldehyde solution is pumped out from the medicament cavity until the formaldehyde solution submerges the root of the tissue culture seedling, the timing function of the timing water pump is started, and the pumping time is set;
4) And after the set time is reached, the formaldehyde solution in the cleaning soaking tank is pumped out by the timing water pump, the placing discs in the cleaning soaking tank are sequentially taken out, and transplanting is started.
The invention has the following advantages:
1. the water tank and the cleaning soaking tank are arranged on the movable frame, and clean water flows in the cleaning soaking tank, so that the roots of a plurality of tissue culture seedlings in the cleaning soaking tank are washed, the waste time of manual running water washing is reduced, the cleaning effect is improved by controlling the cleaning flow rate and time, and the problem that manual cleaning is not thorough is avoided;
2. after the cleaning is finished, formaldehyde solution is sent into the cleaning soaking tank from the water tank, so that the roots of the tissue culture seedlings are soaked in the formaldehyde solution for sterilization, the soaking time is controlled through the drain pipe and the timing water pump, and the formaldehyde solution is timely pumped out after the soaking time, so that water loss caused by the soaking exceeding time is avoided;
3. after formaldehyde is extracted, the tissue culture seedlings in the cleaning and soaking tank can be continuously placed in the cleaning and soaking tank for cultivation, excessive moisture can be drained simultaneously, additional management is not needed to be taken out, and the tissue culture seedlings can be sequentially transplanted at the moment, so that the tissue culture seedlings waiting for transplanting are prevented from being damaged due to improper management;
4. the cleaning process and the soaking process can be automatically completed in the moving process of the moving frame, and operators only need to operate seedling placement, seedling taking and seedling planting, so that the time for tissue culture and breeding is greatly shortened.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic structural view of the present invention in a use state.
Fig. 3 is a schematic structural view of the cleaning soaking tank.
Fig. 4 is a schematic view of the structure of the cleaning soaking tank in a use state.
Fig. 5 is a schematic view of the structure of the cleaning soaking tank in another use state.
Fig. 6 is a schematic side view of a cleaning soaking tank.
Fig. 7 is a schematic view of a structure of the placement tray.
Fig. 8 is a schematic top view of the water tank.
In the figure: 1. a moving rack; 2. a walking mechanism; 3. a water tank; 4. a clear water cavity; 5. a medicament chamber; 6. a waste water chamber; 7. cleaning a soaking tank; 8. tissue culture seedlings; 9. a drain pipe; 10. a timing water pump; 11. a water inlet pipe; 12. placing a tray; 13. inserting seedling holes; 14. a filter screen; 15. a groove; 16. sealing the door; 17. a slide rail; 18. a support rail; 19. a main water pipe; 20. a main drain pipe; 21. a first electromagnetic valve; 22. a second electromagnetic valve; 23. a first liquid pump; 24. and a second liquid pump.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without collision.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, or are directions or positional relationships conventionally understood by those skilled in the art, are merely for convenience of describing the present invention and for simplifying the description, and are not to indicate or imply that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1: as shown in fig. 1 to 8, a potato plant tissue culture seedling 8 cutting propagation transfer device comprises a movable frame 1; the bottom of the movable frame 1 is provided with a travelling mechanism 2; the movable frame 1 is provided with a water tank 3; the water tank 3 is internally divided into a clear water cavity 4, a medicament cavity 5 and a waste water cavity 6 by a partition board group; the movable frame 1 is also provided with a cleaning soaking tank 7; the cleaning soaking tank 7 is connected with the water tank 3; tissue culture seedlings 8 are placed in the cleaning soaking tank 7 at intervals; the cleaning soaking tank 7 is used for cleaning 8 tissue culture seedlings when clear water flows in the cleaning soaking tank; the cleaning soaking tank 7 is used for soaking 8 tissue culture seedlings when the cleaning soaking tank contains a medicament; the bottom of the cleaning soaking tank 7 is connected with a drain pipe 9; the drain pipe 9 is provided with a timing water pump 10. In the embodiment, the movable frame 1 is made of a material with certain mechanical strength, and any reasonable material can be selected according to the requirement; in the embodiment, the travelling mechanism 2 adopts a conventional structure, such as a universal wheel or a travelling wheel driven to move by a motor; in the embodiment, the partition board group in the water tank 3 comprises vertical partition boards, the interior of the water tank 3 is divided into a water inlet area and a water outlet area, and the water inlet area is the waste water cavity 6; an L-shaped partition plate is arranged in the water outlet area to divide the water outlet area into a clear water cavity 4 and a medicament cavity 5; the medicament cavity 5 and the clean water cavity 4 can be connected through a pipeline, and a pump and a check valve are arranged on the pipeline, so that clean water in the clean water cavity 4 is pumped into the medicament cavity 5, and medicament in the medicament cavity 5 is diluted. The bottom of the waste water cavity 6 is provided with a water outlet, the top of the clean water cavity 4 is provided with a water supplementing port, and the top of the medicament cavity 5 is provided with a medicament adding port.
The water tank 3 and the cleaning soaking tank 7 are arranged on the movable frame 1, and clean water flows in the cleaning soaking tank 7, so that the roots of a plurality of tissue culture seedlings 8 in the cleaning soaking tank 7 are washed, the time wasted by manual running water washing is reduced, the cleaning effect is improved by controlling the cleaning flow rate and time, and the problem that manual cleaning is not thorough is avoided; after the cleaning is finished, formaldehyde solution is sent into the cleaning soaking tank 7 from the water tank 3, so that the root of the tissue culture seedling 8 is soaked in the formaldehyde solution for sterilization, the soaking time is controlled through the drain pipe 9 and the timing water pump 10, and after the soaking time, the formaldehyde solution is timely pumped out, so that water loss caused by soaking exceeding time is avoided; after formaldehyde is extracted, the tissue culture seedlings 8 in the cleaning and soaking tank 7 can be continuously placed in the cleaning and soaking tank for cultivation, meanwhile, excessive moisture can be drained, additional management is not needed to be taken out, at the moment, the tissue culture seedlings 8 can be sequentially transplanted, and damage of the tissue culture seedlings 8 waiting for transplanting due to improper management is avoided; the cleaning process and the soaking process can be automatically completed in the moving process of the moving frame 1, and operators only need to operate seedling placement, seedling taking and seedling planting, so that the time for tissue culture and breeding is greatly shortened.
A water inlet pipe 11 is arranged at one end of the bottom of the cleaning soaking tank 7; the drain pipe 9 is arranged at the other end of the bottom of the cleaning soaking tank 7; a placing disc 12 is movably arranged in the cleaning soaking tank 7; seedling inserting holes 13 are formed in the placing plate 12 at intervals; in the embodiment, the water inlet pipe 11 is beneficial to introducing clean water or formaldehyde solution into the cleaning and soaking tank 7, the water outlet pipe 9 is normally opened during cleaning, so that the clean water can flow in the cleaning and soaking tank 7, the water outlet pipe 9 is closed during soaking the root of the tissue culture seedling 8, the water outlet pipe 9 is used for discharging water after the soaking is finished, and the root of the tissue culture seedling 8 is timely separated from the formaldehyde solution; the placing tray 12 is erected in the cleaning and soaking tank 7, the seedling inserting holes 13 on the placing tray 12 are used for penetrating through one tissue culture seedling 8, so that the root is suspended in the cleaning and soaking tank 7 to facilitate cleaning and soaking, and after cleaning and soaking are completed, the tissue culture seedling 8 is erected in the cleaning and soaking tank 7 through the placing tray 12 to facilitate draining residual formaldehyde solution, and damage to the seedling and the root caused by mutual extrusion is avoided, so that the survival rate of the tissue culture seedling 8 is improved.
A filter screen 14 is arranged between the placing plate 12 and the bottom of the cleaning soaking tank 7; the filter screen 14 is arranged between the placing plate 12 and the cleaning soaking tank 7, so that fixed impurities can be prevented from being carried out of the drain pipe 9, the drain pipe 9 is prevented from being blocked, and the normal work of the second electromagnetic valve 22 and the timing water pump 10 on the drain pipe 9 is prevented from being influenced.
A groove 15 is formed in one end face of the cleaning soaking tank 7; the groove 15 is hinged with a sealing door 16; a waterproof rubber ring is arranged on the sealing door 16; in the embodiment, after the groove 15 is arranged on the end face of the cleaning soaking tank 7, the placing tray 12 is conveniently pulled out of the groove 15 during transplanting, and the tissue culture seedlings 8 are prevented from being taken out; the sealing door 16 is arranged on the groove 15, and the sealing door 16 is closed during cleaning and soaking, so that the cleaning and soaking tank 7 is prevented from leaking water.
Slide rails 17 are arranged on the two side walls of the cleaning soaking tank 7 at intervals; the two sides of the placing plate 12 are provided with supporting rails corresponding to the sliding rails 17; the sliding rail 17 and the supporting rail enable the placing groove to be drawn out from the groove 15, and tissue culture seedlings 8 can be conveniently taken out from the cleaning soaking groove 7.
A plurality of layers of cleaning soaking tanks 7 are arranged on the movable frame 1 at intervals; the water inlet pipe 11 is connected to the clean water cavity 4 through a main water pipe 19; the drain pipe 9 is connected to the waste water chamber 6 through a main drain pipe 20; the main water pipe 19 is connected with a medicament pipe through a tee joint; the medicament tube is connected to the medicament lumen 5; in this embodiment, the cleaning and soaking tanks 7 may be provided with multiple layers, the main water pipe 19 sends the clean water or formaldehyde solution into each layer of cleaning and soaking tank 7 through the water inlet pipe 11, and the drain pipe 9 sends the waste water into the main drain pipe 20 and then discharges into the waste water cavity 6.
The water inlet pipe 11 is provided with a first electromagnetic valve 21; a second electromagnetic valve 22 is arranged on the drain pipe 9; the main water pipe 19 is provided with a first liquid pump 23; a second liquid pump 24 is provided on the medicament tube. In this embodiment, the timing water pump 10, the first electromagnetic valve 21, the second electromagnetic valve 22, the first liquid pump 23 and the second liquid pump 24 can be connected to a conventional numerical control system, such as a PLC numerical control system, for simple numerical control coordination.
Example 2: a method for transferring the tissue culture seedling 8 of potato plants, wherein the apparatus for transferring the tissue culture seedling 8 of potato plants in the embodiment 1 is provided;
the method comprises the following steps:
1) The tissue culture seedlings 8 are inserted into the seedling inserting holes 13 on the placing tray 12, and the placing tray 12 is placed into the cleaning soaking tank 7;
2) When the movable frame 1 moves to a transplanting greenhouse, a first liquid pump 23 on a main water pipe 19, a first electromagnetic valve 21 on a water inlet pipe 11, a second electromagnetic valve 22 on a water outlet pipe 9 and a timing water pump 10 are opened, clear water is pumped out from a clear water cavity 4 in the water tank 3, the clear water flows in the cleaning soaking tank 7, and agar remained at the root of the tissue culture seedling 8 is cleaned;
3) After the cleaning is finished, the first liquid pump 23, the second electromagnetic valve 22 and the timing water pump 10 are closed; a second liquid pump 24 on the medicament tube is started, formaldehyde solution is pumped out from the medicament cavity 5 until the formaldehyde solution submerges the root of the tissue culture seedling 8, the timing function of the timing water pump 10 is started, and the water pumping time is set;
4) After the set time is reached, the timing water pump 10 pumps out formaldehyde solution in the cleaning soaking tank 7, and the placing plates 12 in the cleaning soaking tank 7 are sequentially taken out to start transplanting.
In the embodiment, the flow rate is controlled to be 0.45-0.65m/s when the clean water is cleaned; the cleaning time of the clean water is 10-15min; the concentration of the formaldehyde solution is 0.1%, and the soaking time of the formaldehyde solution is 8-12min.
Example 3: the influence of different potato plant tissue culture seedling 8 cutting propagation transfer methods on the survival rate of the potato tissue culture seedling 8.
Selecting potato blocks with smooth surfaces, uniform sizes, no plant diseases and insect pests and no damage, culturing stem tip meristem to obtain detoxified seedlings, hardening the seedlings, and transferring.
Experiment group 1: manually washing the tissue culture seedlings 8 with tap water for 10-15min, soaking 50 groups of tissue culture seedlings in formaldehyde solution for 10min, taking out after soaking, and immediately transplanting.
Experiment group 2: the potato plant tissue culture seedling 8 cutting propagation transfer device of the embodiment 1 and the method of the embodiment 2 are adopted, and the flow rate is controlled to be 0.45m/s when clean water is used for cleaning; the clean water cleaning time is 10min; the concentration of the formaldehyde solution is 0.1%, and the soaking time of the formaldehyde solution is 8min.
Experiment group 3: the potato plant tissue culture seedling 8 cutting propagation transfer device of the embodiment 1 and the method of the embodiment 2 are adopted, and the flow rate is controlled to be 0.65m/s when clean water is used for cleaning; the clean water cleaning time is 15min; the concentration of the formaldehyde solution is 0.1%, and the soaking time of the formaldehyde solution is 12min.
Experiment group 4: the potato plant tissue culture seedling 8 cutting propagation transfer device of the embodiment 1 and the method of the embodiment 2 are adopted, and the flow rate is controlled to be 0.50m/s when clean water is used for cleaning; the clean water cleaning time is 12min; the concentration of the formaldehyde solution is 0.1%, and the soaking time of the formaldehyde solution is 10min.
Experimental group 5: the potato plant tissue culture seedling 8 cutting propagation transfer device of the embodiment 1 and the method of the embodiment 2 are adopted, and the flow rate is controlled to be 0.50m/s when clean water is used for cleaning; the clean water cleaning time is 12min; the concentration of the formaldehyde solution is 0.1%, and the soaking time of the formaldehyde solution is 30min.
The survival results for experimental groups 1-5 are shown in table 1 below.
TABLE 1 influence of different methods for cutting propagation and transfer of tissue culture seedlings 8 of potato plants on survival rate of tissue culture seedlings 8 of potato plants
| Experimental group | Experiment group 1 | Experiment group 2 | Experiment group 3 | Experiment group 4 | Experiment group 5 |
| Survival rate | 85% | 94% | 95% | 98% | 67% |
Although the present invention has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present invention.
Claims (9)
1. A potato plant tissue culture seedling cutting propagation transfer device comprises a movable frame; the bottom of the movable frame is provided with a travelling mechanism; the method is characterized in that: the movable frame is provided with a water tank; the water tank is internally divided into a clear water cavity, a medicament cavity and a waste water cavity by a partition plate group; the movable frame is also provided with a cleaning soaking tank; the cleaning soaking tank is connected with the water tank; tissue culture seedlings are placed in the cleaning soaking tank at intervals; the cleaning soaking tank is used for cleaning tissue culture seedling roots when clear water flows in the cleaning soaking tank; the cleaning soaking tank is used for soaking the tissue culture seedling roots when the cleaning soaking tank contains a medicament; the bottom of the cleaning soaking tank is connected with a drain pipe; the drain pipe is provided with a timing water pump.
2. The potato plant tissue culture seedling cutting propagation transfer device according to claim 1, wherein: a water inlet pipe is arranged at one end of the bottom of the cleaning soaking tank; the drain pipe is arranged at the other end of the bottom of the cleaning soaking tank; a placing disc is movably arranged in the cleaning soaking tank; seedling inserting holes are formed in the placing plate at intervals.
3. The potato plant tissue culture seedling cutting propagation transfer device according to claim 2, wherein: a filter screen is arranged between the placing plate and the bottom of the cleaning soaking tank.
4. The potato plant tissue culture seedling cutting propagation transfer device according to claim 2, wherein: a groove is formed in one end face of the cleaning soaking tank; the groove is hinged with a sealing door; the sealing door is provided with a waterproof rubber ring.
5. The potato plant tissue culture seedling cutting propagation transfer device of claim 4, wherein: slide rails are arranged on the two side walls of the cleaning soaking tank at intervals; the two sides of the placing plate are provided with supporting rails corresponding to the sliding rails.
6. The potato plant tissue culture seedling cutting propagation transfer device according to claim 1, wherein: a plurality of layers of cleaning soaking tanks are arranged on the movable frame at intervals; the water inlet pipe is connected to the clean water cavity through a main water pipe; the drain pipe is connected to the waste water cavity through a main drain pipe; the main water pipe is connected with a medicament pipe through a tee joint; the medicament tube is connected to the medicament lumen.
7. The potato plant tissue culture seedling cutting propagation transfer device of claim 6, wherein: the water inlet pipe is provided with a first electromagnetic valve; the drain pipe is provided with a second electromagnetic valve; a first liquid pump is arranged on the main water pipe; the medicament tube is provided with a second liquid pump.
8. A potato plant tissue culture seedling cutting propagation transfer method is characterized in that: a potato plant tissue culture seedling cutting propagation transfer device as claimed in any one of claims 1-8.
9. The method for cutting, propagating and transferring potato plant tissue culture seedlings according to claim 8, wherein the method comprises the following steps: the method comprises the following steps:
1) The tissue culture seedlings are inserted into the seedling inserting holes on the placing tray, and the placing tray is placed into the cleaning soaking tank;
2) When the movable frame moves to the transplanting greenhouse, a first liquid pump on the main water pipe, a first electromagnetic valve on the water inlet pipe, a second electromagnetic valve on the drain pipe and a timing water pump are opened, clear water is pumped out from a clear water cavity in the water tank, the clear water flows in the cleaning soaking tank, and agar remained at the root of the tissue culture seedling is cleaned;
3) After the cleaning is finished, the first liquid pump, the second electromagnetic valve and the timing water pump are closed; a second liquid pump on the medicament tube is started, formaldehyde solution is pumped out from the medicament cavity until the formaldehyde solution submerges the root of the tissue culture seedling, the timing function of the timing water pump is started, and the pumping time is set;
4) And after the set time is reached, the formaldehyde solution in the cleaning soaking tank is pumped out by the timing water pump, the placing discs in the cleaning soaking tank are sequentially taken out, and transplanting is started.
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| CN202310177420.6A CN116210590A (en) | 2023-02-28 | 2023-02-28 | Potato plant tissue culture seedling cutting propagation transfer device and transfer method thereof |
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| CN116369208A (en) * | 2023-06-07 | 2023-07-04 | 包头市农牧科学技术研究所 | Tissue culture and rapid propagation method for hippeastrum henryi and bulb pretreatment device thereof |
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| CN114946658A (en) * | 2022-06-06 | 2022-08-30 | 贵州省生物技术研究所(贵州省生物技术重点实验室、贵州省马铃薯研究所、贵州省食品加工研究所) | Disinfection and sterilization method for rapid tissue culture of sweet potatoes |
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| CN209861754U (en) * | 2019-03-18 | 2019-12-31 | 南京晶薯生物科技有限公司 | Sweet potato seedling culture device capable of improving transplanting survival rate |
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| CN113455319A (en) * | 2021-06-21 | 2021-10-01 | 百色学院 | Transplanting method of cassava tissue culture contaminated seedlings |
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