CN107258538A - A kind of apple rootstock SH38 rapid propagation methods - Google Patents
A kind of apple rootstock SH38 rapid propagation methods Download PDFInfo
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- CN107258538A CN107258538A CN201710529147.3A CN201710529147A CN107258538A CN 107258538 A CN107258538 A CN 107258538A CN 201710529147 A CN201710529147 A CN 201710529147A CN 107258538 A CN107258538 A CN 107258538A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention belongs to apple nursery plant cultivation technology field, a kind of apple rootstock SH38 rapid propagation methods are disclosed, by using culture medium Initial culture, squamous subculture using MS+6 BA 0.6mg/L+IBA 0.1mg/L processing, culture of rootage and then acclimatization and transplantses with pure sawdust using root media 1/2MS+IBA1.2mg/L+ epiphysin 0.03mg/L of the culture medium for MS+6 BA0.6mg/L+IBA0.1mg/L+PVP 500mg/L；Realize apple rootstock quickly to breed, not only ensure that higher survival rate, and remain the good characteristic of stock, the development and further expansion for Apple Industry provide easily method and approach.The present invention sets up a kind of apple rootstock SH38 rapid propagation methods, and virus-free nursery stock, significant, wide market are downgraded for production high-quality.
Description
Technical field
The invention belongs to apple nursery plant cultivation technology field, more particularly to a kind of apple rootstock SH38 rapid propagation methods.
Background technology
SH systems are to be passed through by Research Inst. for fruit Tree, Agricultural Academy of Shanxi Prov. using cross breeding method (state's light × Henan Malus spectabilis)
16 years (1978-1993) research seed selection is crossed to form.Identification, wherein SH40, SH6, SH1 tri- anvils number are observed through system for many years
Integral economic trait has exceeded the Apples Dwarf Stocks such as M, MM, P system of foreign countries, and 13, the whole nation, province's examination Extend culture phene is dashed forward
Go out, stably.At present, the apple production pattern of China by traditional poorly efficient closing orchard pattern progressively to be changed into the short anvil of modernization close
Plant intensive efficient production cultivation mode and made the country prosperous by the Apple Industry that is changed into of apple production big country of the world progressively.Apple nursery stock
The basis of apple production cultivation, the quality of nursery stock directly affect the growth of apple tree, bloom, result and resistance,
Resistance against diseases and life-span.After nursery stock is infected by virus, the normal cell propagation of tree body will be destroyed, serious
Words can influence the normal physiological mechanism such as growth potential, fruit quality and yield of fruit tree, and this turns into the hidden of Chinese apple industry development
Suffer from.One period is the critical period that China's apple updates on a large scale from now on, how to be provided in terms of nursery stock and supports and protect
Barrier becomes the problem of being worth paying much attention to for one.
In summary, the problem of prior art is present be：
The poor Dwarf Stocks For Apple Trees SH systems of the adaptability of acclimatization and transplantses later stage dwarfing stock 1. (such as SH38, SH40) Root Distribution
Shallow, this requires preferable fertilizer and water condition.
2. transplanting behind crop field, the easy undergrowth early growths of Dwarf Stocks For Apple Trees SH38 are excessively weak, are difficult to form yield, as a result
Flower and fruit thinning is not in place afterwards, biennial bearing easily occurs, thus can not show the advantage of dwarfing rootstock dense planting.
3. it is high to set up Dwarf Stocks For Apple Trees tissue-cultured seedling rapid propagation system time length, cost, cost increases, and increases building well throwing
Money, influences whether the application of some orchard workers.
The content of the invention
The problem of existing for prior art, the invention provides a kind of apple rootstock SH38 rapid propagation methods.
The present invention is achieved in that a kind of apple rootstock SH38 rapid propagation methods, and the apple rootstock SH38 is quick
Propagation method is by using the culture medium Initial culture that culture medium is MS+6-BA0.6mg/L+IBA0.1mg/L+PVP500mg/L；
The squamous subculture handled using MS+6-BA0.6mg/L+IBA0.1mg/L；Taken off using root media 1/2MS+IBA1.2mg/L+
Melanocyte 0.03mg/L culture of rootage；Then realize that apple rootstock is quickly bred with the acclimatization and transplantses of pure sawdust.
Further, the apple rootstock SH38 rapid propagation methods comprise the following steps：
Step one, using apple rootstock SH38 as material, the elite stand of growth selection stalwartness no disease and pests harm takes life in 1 year March
Branch, places and water planting is carried out in 26 DEG C of greenhouse, and water planting is that every liter of water contains 5g sucrose, changes a water every 5d and cuts old cut
Mouthful；
Step 2, cleans 3min with supersonic wave cleaning machine, then with aseptic water washing 3-5 times, the tender shoots handled well is inoculated with
Adjoin to polyethylene and cultivated in pyrrolidone 500mg/L culture medium, the additional 30g sucrose of every liter of culture medium, 7g agar；
Step 3, MS+6-BA0.6mg/L+IBA0.1mg/L, illumination 2000lux are inoculated into by the aseptic explant of acquisition
Culture medium in carry out squamous subculture；
Step 4, the robust growth that selection squamous subculture is obtained, the highly plant more than 2.0cm, growth time are 25 days
Bud, be inoculated in 1/2MS+IBA1.2mg/L+ epiphysin 0.03mg/L root medias and carry out culture of rootage；
Step 5, after tissue-cultured seedling root length reaches 2cm, moves on to outdoor and shelters from heat or light hardening 10-12 days, then by culture vessel
Bottleneck is opened, and corkage hardening is carried out under natural light after 2-4 days, rooted seedling is taken out from culture medium, it is more than 0.1% to be put into concentration
5min is soaked in bacterium spirit solution, and the unnecessary culture medium in root is cleaned up, is finally rinsed 2-3 times with clear water, then with pure
In the nutritive cube that sawdust is transplanted to.
Further, in the step one：When the bud pumping on the annotinous branch of water planting is 1.5-2.0cm, clip is tender
Bud, removes expansion blade, 6-8h is rinsed under running water, with 75% ethanol postincubation 30S, mercuric chloride processing 7min.
Further, in the step 2：It is placed under the conditions of illumination 16h/d, illumination 2000lux, 26 DEG C and is cultivated.
Further, in the step 3：SH38 optimal culture is processed as M5 processing, every liter of additional 30g sucrose, 7g fine jades
Fat.
Further, in the step 5：The way to manage of transplanted seedling is to be managed rooted seedling under shade net after transplanting
Reason, uses concentration to be sprayed for 0.1% carbendazim solution in first week, and second week is sprayed once with clear water daily, keeps matrix
The ventilative offspring pumping to be generated of moistening goes out i.e. extensible shade net after sprouting.
Another object of the present invention is to provide a kind of apple bred using the apple rootstock SH38 rapid propagation methods
Fruit stock SH38.
Advantages of the present invention and good effect are：It is MS+6-BA0.6mg/L+IBA0.1mg/L+ by using culture medium
PVP 500mg/L culture medium Initial culture, using MS+6-BA 0.6mg/L+IBA 0.1mg/L handle squamous subculture, adopt
With root media 1/2MS+IBA1.2mg/L+ epiphysins 0.03mg/L culture of rootage and then the acclimatization and transplantses with pure sawdust；
Realize apple rootstock quickly to breed, not only ensure that higher survival rate, and remain the good characteristic of stock, be
The development of Apple Industry and further expansion provide easily method and approach.It is fast that the present invention sets up a kind of apple rootstock SH38
The method of speed breeding, virus-free nursery stock, significant, wide market are downgraded for production high-quality.
The present invention compared with prior art has advantages below：
(1) epiphysin and the proportionings of auxin IBA suitably, effectively facilitate tissue culture seedling rooting, adapt to grown in field in the future.
(2) tissue-cultured seedling of health is provided for crop field, then coordinates corresponding administrative skill, dwarfing rootstock is at utmost played close
The advantage of plant.
(3) when this decimetre sets up primary, sterilized using 75% alcohol disinfecting 30S+0.1% mercuric chloride, it is ensured that explant
Survival rate, reduces pollution rate, melting brown rate, it is to avoid explant is set up in repetition, is that a whole set of rapid propagation system reduces time and cost.
Brief description of the drawings
Fig. 1 is apple rootstock SH38 rapid propagation method flow charts provided in an embodiment of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in figure 1, apple rootstock SH38 rapid propagation methods provided in an embodiment of the present invention comprise the following steps：
S101：Using apple rootstock SH38 as material, the elite stand of growth selection stalwartness no disease and pests harm takes 1 year raw branch March
Bar, places and water planting is carried out in 26 DEG C of greenhouse, and water planting is that every liter of water contains 5g sucrose, changes a water every 5d and cuts old cut
Mouthful, when the bud pumping on the annotinous branch of water planting is 1.5-2.0cm, clip tender shoots removes expansion blade, under running water
6-8h is rinsed, with 75% ethanol postincubation 30S, mercuric chloride processing 7min；
S102：Clean 3min with supersonic wave cleaning machine, then with aseptic water washing 3-5 times, the tender shoots handled well is inoculated into
Polyethylene adjoins to be cultivated in pyrrolidone 500mg/L culture medium, and then the additional 30g sucrose of every liter of culture medium, 7g agar placed
Cultivated under the conditions of illumination 16h/d, illumination 2000lux, 26 DEG C；
S103：The aseptic explant of acquisition is inoculated into MS+6-BA0.6mg/L+IBA0.1mg/L, illumination 2000lux's
Squamous subculture is carried out in culture medium, SH38 optimal culture is processed as M5 processing, i.e., every liter additional 30g sucrose, 7g agar；
S104：The robust growth for selecting squamous subculture to obtain, the highly plant more than 2.0cm, growth time are 25 days
Bud, is inoculated in 1/2MS+IBA1.2mg/L+ epiphysin 0.03mg/L root medias and carries out culture of rootage, every liter plus 30g
Sucrose, 7g agar；
S105：After tissue-cultured seedling root length reaches 2cm, move on to outdoor and shelter from heat or light hardening 10-12 days, then by culture vessel bottle
Mouth is opened, and corkage hardening is carried out under natural light after 2-4 days, rooted seedling is taken out from culture medium, concentration is put into for bacterium more than 0.1%
5min is soaked in clever solution, and the unnecessary culture medium in root is cleaned up, is finally rinsed 2-3 times with clear water, then with pure saw
In the nutritive cube that end is transplanted to.
In step S105, rooted seedling is is managed by the way to manage of transplanted seedling under shade net after transplanting, and first
Zhou Caiyong concentration is that 0.1% carbendazim solution is sprayed, and second week is sprayed once with clear water daily, keeps matrix moistening ventilative
Offspring pumping to be generated goes out i.e. extensible shade net after sprouting.
Apple rootstock SH38 rapid propagation methods provided in an embodiment of the present invention specifically include following steps：
Step one, Initial culture
Apple rootstock SH38 is material, and the elite stand of growth selection stalwartness no disease and pests harm takes 1 year raw branch March, placed
Water planting (every liter of water contains 5g sucrose) is carried out in 26 DEG C of greenhouse, a water is changed every 5d and cuts old clip, when the one of water planting
When bud pumping on year life branch is 1.5-2.0cm, clip tender shoots removes expansion blade, 6-8h is rinsed under running water, use
75% ethanol postincubation 8s, 0.1% mercuric chloride processing 7min, after the processing that carries out disinfection, cleans 3min with supersonic wave cleaning machine, then with nothing
Bacterium water rinse 3-5 time, by the tender shoots handled well be inoculated into MS+6-BA0.6mg/L+IBA0.1mg/L+ various concentrations handle (0,
0.3rd, 0.5,0.7,1.0g/L) polyethylene adjoin and cultivated in pyrrolidone (pvp) culture medium, the additional 30g of every liter of culture medium
Sucrose, 8g agar, each triangular flask connects 3 buds, often handles 15 bottles, is then placed within illumination 16h/d, illumination 2000lux, 26 DEG C
Under the conditions of cultivated, pollution rate, melting brown rate, survival rate are investigated after 14d respectively, the SH38 Initial cultures drawn are optimum
Culture medium is MS+6-BA0.6mg/L+IBA0.1mg/L+PVP500mg/L (sucrose 30g/L, agar 7g/L)；
Step 2, squamous subculture
By the aseptic explant of acquisition be inoculated into containing carried out in different 6-BA, agar, hormone, the MS culture mediums of illumination after
It is commissioned to train foster, every liter of additional 30g sucrose, 8g agar, 3 plants every bottle, each 10 bottles of processing is repeated 3 times, SH38 optimal culture processing
For M5 processing, i.e. MS+6-BA0.6mg/L+IBA0.1mg/L (agar 7g/L, illumination 2000lux)；
Step 3, culture of rootage
The robust growth for selecting squamous subculture to obtain, the highly plant more than 1.5cm, growth time are bud of 25 days,
Be inoculated in 1/2MS+ various concentrations IBA processing (0,0.6,0.8,1.2,1.4mg/L) culture of rootage in carry out culture of rootage, often
Bottle inoculation 3, each 10 bottles of processing, is repeated 3 times, and obtains the optimum root medias of SH38 for 1/2MS+IBA1.2mg/L+
Epiphysin 0.03mg/L (sucrose 30g/L, agar 7g/L)；
Step 4, acclimatization and transplantses
After tissue-cultured seedling root length reaches 2cm, move on to outdoor and shelter from heat or light hardening 10-12 days or so, then by culture vessel bottle
Mouth is opened, and corkage hardening is carried out under natural light after 2-4 days, rooted seedling is taken out from culture medium, concentration is put into for bacterium more than 0.1%
5min is soaked in clever solution, and the unnecessary culture medium in root is cleaned up, is finally rinsed 2-3 times, is then transplanted to clear water
The processing of capacity identical different substrates (vermiculite, pure sawdust, field soil, the 3/5 field soil+vermiculite of top 2/5,3/5 field soil+2/5
Perlite) nutritive cube in, the way to manage of transplanted seedling uses concentration in first week for rooted seedling is managed under shade net
Sprayed for 0.1% carbendazim solution, second week is sprayed once with water daily, keep matrix moistening ventilative.In system after 14 days
Acclimatization and transplantses survival rate is counted, is drawn best with pure sawdust acclimatization and transplantses survival rate.
The application effect of the present invention is described further with reference to experiment and data.
1st, the influence that the various concentrations PVP of reference table 1 processing grows to explant；
The influence that the various concentrations PVP of table 1 processing grows to explant
| PVP concentration | SH38 |
| Pollution rate/% | Melting brown rate/% | Survival rate/% | |
| 0 | 79.1±2.35a | 14.34±1.25bc | 19.34±1.34b |
| 300mg/L | 74.34±2.25a | 14.45±1.01bc | 17.35±1.12c |
| 500mg/L | 62.83±2.13c | 11.14±1.23c | 35.45±1.10a |
| 700mg/L | 81.05±2.44b | 20.56±1.02a | 16.45±1.13b |
| 1.0mg/L | 84.06±2.81a | 17.24±0.87b | 14.53±0.81c |
Note represents difference not significantly (p with columns same letter>0.05), different letters represent significant difference (p<0.05).
Data are Mean ± SE (average value ± standard error)；Similarly hereinafter.
2nd, difference 6-BA concentration handles the influence to SH38 squamous subcultures：
Influence of the difference 6-BA concentration of table 2 to SH38 squamous subcultures
| Minimal medium | 6-BA(mg/L) | IBA(mg/L) | Expand numerous coefficient |
| MS | 0 | 0.1 | 1.22±0.02c |
| MS | 0.3 | 0.1 | 1.06±0.03c |
| MS | 0.4 | 0.1 | 2.37±0.34b |
| MS | 0.5 | 0.1 | 5.01±0.08a |
| MS | 0.6 | 0.1 | 5.24±0.11a |
3rd, the influence that difference IBA concentration processing SH38 is taken root
The influence that the different work concentration processing of table 3 are taken root
4th, the influence that different hardening matrix grow to rooted seedling：
The influence that the different cultivation matrix processing of table 4 grow to transplanted seedling
| Matrix treatments | SH38 transplanting survival rates |
| Vermiculite | 72.50±4.20a |
| Pure sawdust | 82.00±4.33a |
| Field soil | 68.50±3.42b |
| 3/5 field+2/5 vermiculite of soil | 67.00±2.33a |
| 3/5 field+2/5 perlite of soil | 71.00±3.45bc |
5th, the screening of apple rootstock SH38 proliferated culture mediums
By SH38 tissue culture plant inoculations in the culture medium containing different 6-BA concentration hormones, (sucrose concentration 30g/L, agar is dense
Spend 7g/L, pH5.8) in, the numerous coefficient of expansion under various concentrations hormone, vitrifying degree and growing state are observed, 30 are each handled
Bottle, 3 plants every bottle.
6th, influence of the agar concentration to SH38 Vitrifications：
The result of the test of observational technique 2., according to general performances such as the numerous coefficients of the expansion of tissue-cultured seedling, select it is relatively good after
Control, influence of the research agar concentration to Vitrification are used as culture medium.SH38 tissue culture plant inoculation is contained into difference
Agar concentration (5.0,6.0,7.0g/L), coefficient of differentiation, vitrifying degree and the life of tissue-cultured seedling under observation various concentrations agar
Long situation, each 30 bottles, 3 plants every bottle of processing.
7th, influences of the IBA to SH38 Vitrifications：
The result of the test of observational technique 3., according to general performances such as the numerous coefficients of the expansion of tissue-cultured seedling, select it is relatively suitable after
Compared for culture medium, and with this, research 6-BA and various concentrations IBA matches somebody with somebody the influence for comparing vitrifying degree.Observe tissue-cultured seedling
Coefficient of differentiation, vitrifying degree and growing state, each 30 bottles, 3 plants every bottle of processing.
8th, result and analysis：
8.1st, the screening of apple rootstock SH38 proliferated culture mediums：
As shown in table 5, it is SH38 tissue culture seedling and propagating coefficients in MS+6-BA0.6mg/L+IBA0.1mg/L that M5, which handles culture medium,
Up to 3.64, glass rate is 31%.The vitrifying degree of each processing is directly higher without significant difference, but all.To expand
Numerous coefficient screens for index, then it is preferable proliferated culture medium that M5, which handles culture medium,.
The proliferated culture medium the selection result of table 5 is counted
| Numbering | Culture medium | IBA(mg/L) | 6-BA(mg/L) | Expand numerous coefficient | Vitrifying degree |
| M1 | MS | 0 | 0 | 0 | 0 |
| M2 | MS | 0.1 | 0.3 | 2.78 | 0.31 |
| M3 | MS | 0.1 | 0.4 | 3.1 | 0.34 |
| M4 | MS | 0.1 | 0.5 | 3.42 | 0.37 |
| M5 | MS | 0.1 | 0.6 | 3.64 | 0.31 |
8.2nd, influence of the agar concentration to SH38 Vitrifications
As shown in table 6, drawn using handling culture medium M5 as control, improve agar concentration has certain to Vitrification
Inhibitory action, and improve the numerous coefficient of expansion to a certain extent, but no significant difference difference.Consider, M5 processing cultures
Base is better than other processing, and it is 3.64 that it, which expands numerous coefficient, and glass rate is 31%.
Influence of the agar concentration of table 6 to SH38 tissue-cultured seedling
| M1 | MS | 0 | 0 | 0 | 0 |
| M2 | MS | 0.1 | 0.3 | 2.78 | 0.31 |
| M3 | MS | 0.1 | 0.4 | 3.1 | 0.34 |
| M4 | MS | 0.1 | 0.5 | 3.42 | 0.37 |
| M5 | MS | 0.1 | 0.6 | 3.64 | 0.31 |
8.3rd, influences of the IBA to SH38 Vitrifications
Culture medium is handled as control using M12, influence of the different IBA concentration to SH38 Vitrifications is studied, such as the institute of table 7
Show, a considerable influence can be had to expanding numerous coefficient by improving IBA concentration, processing M7, M9, M10 is improved with compareing that there were significant differences
IBA concentration can significantly reduce vitrifying degree, but expand numerous coefficient and but declined, and consider M7 for more excellent culture medium
Influences of the IBA of table 7 to Vitrification
| Numbering | 6-BA(mg/L) | IBA(mg/L) | Expand numerous coefficient | Vitrifying degree |
| M7 | 0.6 | 0.1 | 3.8 | 0.302 |
| M9 | 0.6 | 0.3 | 3.61 | 0.301 |
| M10 | 0.6 | 0.5 | 3.53 | 0.235 |
| M11 | 0.6 | 0.7 | 3.43 | 0.231 |
| M12 | 0.6 | 0.9 | 3.42 | 0.229 |
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (7)
1. a kind of apple rootstock SH38 rapid propagation methods, it is characterised in that the apple rootstock SH38 rapid propagation methods lead to
Cross the culture medium Initial culture for using culture medium for MS+6-BA0.6mg/L+IBA0.1mg/L+PVP500mg/L；Using MS+6-
The squamous subculture of BA0.6mg/L+IBA0.1mg/L processing；Using root media 1/2MS+IBA1.2mg/L+ epiphysins
0.03mg/L culture of rootage；Then realize that apple rootstock is quickly bred with the acclimatization and transplantses of pure sawdust.
2. apple rootstock SH38 rapid propagation methods as claimed in claim 1, it is characterised in that the apple rootstock SH38 is fast
Fast propagation method comprises the following steps：
Step one, using apple rootstock SH38 as material, the elite stand of growth selection stalwartness no disease and pests harm takes 1 year raw branch March
Bar, places and water planting is carried out in 26 DEG C of greenhouse, and water planting is that every liter of water contains 5g sucrose, changes a water every 5d and cuts old cut
Mouthful；
Step 2, cleans 3min with supersonic wave cleaning machine, then with aseptic water washing 3-5 times, the tender shoots handled well is inoculated into poly-
Ethene adjoins to be cultivated in pyrrolidone 500mg/L culture medium, the additional 30g sucrose of every liter of culture medium, 7g agar；
Step 3, MS+6-BA0.6mg/L+IBA0.1mg/L, illumination 2000lux training are inoculated into by the aseptic explant of acquisition
Support in base and carry out squamous subculture；
Step 4, the robust growth that selection squamous subculture is obtained, the highly plant more than 2.0cm, growth time are the bud of 25 days
Son, is inoculated in 1/2MS+IBA1.2mg/L+ epiphysin 0.03mg/L root medias and carries out culture of rootage；
Step 5, after tissue-cultured seedling root length reaches 2cm, moves on to outdoor and shelters from heat or light hardening 10-12 days, then by culture vessel bottleneck
Open, corkage hardening is carried out under natural light after 2-4 days, rooted seedling is taken out from culture medium, concentration is put into for 0.1% carbendazim
5min is soaked in solution, and the unnecessary culture medium in root is cleaned up, is finally rinsed 2-3 times with clear water, then uses pure sawdust
In the nutritive cube being transplanted to.
3. apple rootstock SH38 rapid propagation methods as claimed in claim 2, it is characterised in that in the step one：Work as water
When bud pumping on the annotinous branch of training is 1.5-2.0cm, clip tender shoots removes expansion blade, 6- is rinsed under running water
8h, with 75% ethanol postincubation 30S, mercuric chloride processing 7min.
4. apple rootstock SH38 rapid propagation methods as claimed in claim 2, it is characterised in that in the step 2：Place
Cultivated under the conditions of illumination 16h/d, illumination 2000lux, 26 DEG C.
5. apple rootstock SH38 rapid propagation methods as claimed in claim 2, it is characterised in that in the step 3：SH38
Optimal culture be processed as M5 processing, every liter of additional 30g sucrose, 7g agar.
6. apple rootstock SH38 rapid propagation methods as claimed in claim 2, it is characterised in that in the step 5：Transplant
The way to manage of seedling uses concentration molten for 0.1% carbendazim in first week to be managed rooted seedling under shade net after transplanting
Liquid is sprayed, and second week is sprayed once with clear water daily, keeps the ventilative offspring pumping to be generated of matrix moistening to go out after sprouting i.e.
Extensible shade net.
7. a kind of usage right requires the apple rootstock of apple rootstock SH38 rapid propagation methods breeding described in 1~6 any one
SH38。
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| CN104839017A (en) * | 2015-04-14 | 2015-08-19 | 西北大学 | Application of melatonin in promotion of rooting and root development of gynura divaricata |
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