CN104839017A - Application of melatonin in promotion of rooting and root development of gynura divaricata - Google Patents
Application of melatonin in promotion of rooting and root development of gynura divaricata Download PDFInfo
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- CN104839017A CN104839017A CN201510176067.5A CN201510176067A CN104839017A CN 104839017 A CN104839017 A CN 104839017A CN 201510176067 A CN201510176067 A CN 201510176067A CN 104839017 A CN104839017 A CN 104839017A
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- epiphysin
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Abstract
The invention discloses an application of melatonin in promotion of rooting and root development of gynura divaricata; well-growing gynura divaricata having a certain length is cut off and then inserted into a melatonin rooting solution with a certain concentration, and the melatonin rooting solution has roles in promoting rooting and root development, so as to overcome a problem that a root cutting rooting period of a gynura divaricata matrix is longer, an obstruct that roots produced in soil cannot be directly used for water culture and a problem that seedling exercising is needed and the seedling exercising death rate is high in a rapid breeding method. Preparation is fast, the method is simple and practical, and melatonin is convenient for mass preparation, applicable in mass culture and remarkable in acting effect; an obtained plant not only can be used as a seedling provided for use in traditional production, but also can be intuitively used for subsequent development of water culture products of gynura divaricata, so that scaling and factorization production are achieved, the land occupation area is saved, the plant quality is standardized, and important application value and practical value are achieved.
Description
Technical field
The invention belongs to novel technical field of cultivation, being specifically related to epiphysin for promoting Radix et Rhizoma Gynurae divaricatae to take root and the application of root development.
Background technology
Radix et Rhizoma Gynurae divaricatae, have another name called golden pheasant hair grass, angelica keiskei koidz, Gynura divaricata etc., mainly be distributed in SOUTHERN CHINA, the areas such as area, the west and south and Taiwan, Radix et Rhizoma Gynurae divaricatae medicinal function is powerful, its root, stem, Ye Junke is used as medicine, root taste is sweet, cool, there is clearing heat and cooling blood, effect of scattered silt detumescence, mainly treating bronchitis, pulmonary tuberculosis, uterine bleeding, carbuncle swells, scald, traumatic injury, knife wound is hemorrhage, in recent years, many compositions in Radix et Rhizoma Gynurae divaricatae are developed research gradually, its medicinal function is subject to people's attention, according to the literature, Flavonoid substances contained by Radix et Rhizoma Gynurae divaricatae has huge medicinal effects, its root, stem and leaf water extract effectively can reduce hypertension, extract has significant protective effect to the major organs damage caused hypertension, and can the symptom such as diabetes-alleviating.The evergreen upright herbaceous plant of Radix et Rhizoma Gynurae divaricatae system composite family Gynura Cass, high 30 ~ 80cm, upright stalk, stem is green, base portion is slightly partially purple, single leaf alternate, meat ellipse or obovate leaf, natural rooting rate is lower, take root a little few, side root is few, and the cycle of taking root is long, there is no relevant research report of taking root at present both at home and abroad.Epiphysin, be melatonin, it is a class indoles tryptamines pigment, find in the pineal gland of animal the earliest, participate in vertebrate Physiological effect, as circadian rhythm and photoperiodic reaction, be usually used in the circadian rhythm disturbances adjusted because jet lag or other dyssomniases cause, improving water flood, treatment neurasthenia etc.Simultaneously, the existence of epiphysin is have also discovered in plant, the growth of epiphysin to plant has regulating action, can by promoting that cells ex vivo expands the effect reaching growth promoting effects, stress resistance of plant can be improved, in addition, research shows, epiphysin has the effect of the regeneration promoting side root and adventive root, epiphysin synthetic gene mainly imports in plant corpus and studies its rooting efficiency by previous research, although can promote that plant corpus is taken root by improving endogenous melatonin content, but its method complexity is not easy to operate, import success rate lower, be not suitable for actual operation in enormous quantities.
Summary of the invention
For defect of the prior art and deficiency; the present invention is shown by a large amount of experimental studies; execute the hestening rooting of the effect epiphysin aqueous solution has to(for) Radix et Rhizoma Gynurae divaricatae outward, be applicable to the standardization of Radix et Rhizoma Gynurae divaricatae, large-scale production, also can be directly used in the product extracting its secondary metabolite.
For achieving the above object, the technical scheme that the present invention takes is:
Epiphysin is for promoting Radix et Rhizoma Gynurae divaricatae to take root and the application of root development.
Concrete, epiphysin solution is for promoting Radix et Rhizoma Gynurae divaricatae to take root and root development, and the concentration of the described epiphysin aqueous solution is 50 ~ 200 μm of ol/L.
More specifically, taking root the time of taking root for shortening Radix et Rhizoma Gynurae divaricatae of described promotion Radix et Rhizoma Gynurae divaricatae, described promotion Radix et Rhizoma Gynurae divaricatae root development is the number that increase Radix et Rhizoma Gynurae divaricatae is taken root.
Concrete again, the time that described Radix et Rhizoma Gynurae divaricatae is taken root is 10 ~ 14 days, and the number that described Radix et Rhizoma Gynurae divaricatae is taken root is 11 ~ 18/stem section.
In addition, epiphysin is for promoting Radix et Rhizoma Gynurae divaricatae to take root and the using method of root development comprises:
At temperature 18 ~ 25 DEG C, relative moisture 55% ~ 70%, intensity of illumination 40 μm of olm
-2s
-1and under the condition that replaces of 16h light irradiation time and 8h lucifuge duration, the position of taking root of the annual semi-lignified branch of Radix et Rhizoma Gynurae divaricatae is immersed in the epiphysin aqueous solution that lucifuge places.
Concrete, the concentration of the described epiphysin aqueous solution is 50 ~ 200 μm of ol/L.
Compared with prior art, the invention has the advantages that:
(1) different to promote it to take root from previously passed plant corpus that epiphysin synthetic gene imported, the present invention by realizing taking root to the dipping of the branch of Radix et Rhizoma Gynurae divaricatae by the aqueous solution of epiphysin in the process of plant cultivation, it is more convenient that additional mode realizes this method, success rate is high, is suitable for large batch of practical operation;
(2) simultaneously, the present invention also finds that the aqueous solution of epiphysin is not all have facilitation to the taking root of branch of all plants, experimental result in embodiment four can be found out, not obvious for the effect that tomorrow, the base of leaf section epiphysin aqueous solution was taken root to it;
(3) Be very effective of the present invention, compounding method is simple, more more efficient and convenient than the preparation of traditional Radix et Rhizoma Gynurae divaricatae taking root liquid, be applicable to prepare in enormous quantities, easy to use, once preparation can be used for cultivating Radix et Rhizoma Gynurae divaricatae in enormous quantities, and cost is low and safe and reliable.
Accompanying drawing explanation
Fig. 1 for contrast stem section (left side) and in culture of rootage liquid process stem section (right side) of 100 μm of ol/L in the take root situation of cultivation after 12 days;
Fig. 2 for contrast stem section (left side) and 100 μm of ol/L culture of rootage liquid process stem section (right side) cultivation after 16 days root grow and lateral root growth situation;
Fig. 3 is that the stem section processed in the culture of rootage liquid of 100 μm of ol/L is directly transplanted in cultivation for 14 days afterwards, survival rate 100%;
Below in conjunction with specification drawings and specific embodiments, the present invention is illustrated.
Embodiment
Because epiphysin is water insoluble, after first dissolving with absolute methanol, be modulated into finite concentration with water again.Use the Radix et Rhizoma Gynurae divaricatae in cultivation one to a year and a half strain age, choose to the youthful and the elderly and have the stem section of 5 ~ 8cm of 6 leaves, cut from its inclined downward, make its unrooted, be inserted in the container containing epiphysin solution, container external application tinfoil wraps up, light decomposes to avoid epiphysin to see, being placed on relative moisture controls 55% ~ 70%, and temperature controls at 18 ~ 25 DEG C, and intensity of illumination is 40 μm of olm
-2s
-1environment under cultivate (16h illumination/8h dark).Variable concentrations epiphysin solution is different on its impact of taking root, and specifically in conjunction with following instance, the present invention is described in detail.
Embodiment 1:
Root-inducing promoter is the aqueous solution containing epiphysin.The concentration of epiphysin in water is 50 μm of ol/L, take 0.058g epiphysin, get appropriate absolute methanol fully to dissolve, 500ml is adjusted to afterwards with water, mix, select Radix et Rhizoma Gynurae divaricatae healthy growth, without damage by disease and insect annual semi-lignified branch, clip is about the stem section that 5cm contains 6 ~ 8 leaves, by its running water 15 ~ 30min, after draining away the water, branch inferior end notch is immersed in sterilization 20min in the carbendazim of 0.1%, be inserted in after taking-up drains away the water in epiphysin solution, under guaranteeing that otch gos deep into liquid level, blake bottle keeps lucifuge state.Being placed in temperature controls at 18 ~ 25 DEG C, relative moisture 55% ~ 70%, and intensity of illumination is 40 μm of olm
-2s
-1, cultivate under the condition of 16h illumination/8h dark.Change the epiphysin aqueous solution after 7 days once, start to take root after 12 days, take root several more, average 11/stem section, and side root is comparatively in great numbers.
Embodiment 2:
Root-inducing promoter is the aqueous solution containing epiphysin.The concentration of epiphysin in water is 100 μm of ol/L, take 0.0116g epiphysin, get appropriate absolute methanol fully to dissolve, 500ml is adjusted to afterwards with water, mix, select Radix et Rhizoma Gynurae divaricatae healthy growth, without damage by disease and insect annual semi-lignified branch, clip is about the stem section that 7cm contains 6 ~ 8 leaves, by its running water 15 ~ 30min, after draining away the water, branch inferior end notch is immersed in sterilization 20min in the carbendazim of 0.1%, be inserted in after taking-up drains away the water in epiphysin solution, under guaranteeing that otch gos deep into liquid level, blake bottle keeps lucifuge state.Being placed in temperature controls at 18 ~ 25 DEG C, relative moisture 55% ~ 70%, and intensity of illumination is 40 μm of olm
-2s
-1, cultivate under the condition of 16h illumination/8h dark.Change the epiphysin aqueous solution after 7 days once, start to take root after 10 days, number of taking root is many, average 18/stem section, and side root is many.
Embodiment 3:
Root-inducing promoter is the aqueous solution containing epiphysin.The concentration of epiphysin in water is 200 μm of ol/L, take 0.023g epiphysin, get appropriate absolute methanol alcohol fully to dissolve, 500ml is adjusted to afterwards with water, mix, select Radix et Rhizoma Gynurae divaricatae healthy growth, without damage by disease and insect annual semi-lignified branch, clip is about the stem section that 7cm contains 6 ~ 8 leaves, by its running water 15 ~ 30min, after draining away the water, branch inferior end notch is immersed in sterilization 20min in the carbendazim of 0.1%, be inserted in after taking-up drains away the water in epiphysin solution, under guaranteeing that otch gos deep into liquid level, blake bottle keeps lucifuge state.Being placed in temperature controls at 18 ~ 25 DEG C, relative moisture 55% ~ 70%, and intensity of illumination is 40 μm of olm
-2s
-1, cultivate under the condition of 16h illumination/8h dark.Change the epiphysin aqueous solution after 7 days once, start to take root after 14 days, take root several more, average 12/stem section, and root is thick, side root is more.
Embodiment 4:
Root-inducing promoter is the aqueous solution containing epiphysin.The concentration of epiphysin in water is 50 μm of ol/L, take 0.058g epiphysin, get appropriate absolute methanol fully to dissolve, 500ml is adjusted to afterwards with water, mix, select the healthy growth of medicinal plant Angelica keiskei (Carrot family angelica (Angelica keiskeiKoidzumi)), without the annotinous branch of damage by disease and insect, clip is about the stem section that 5cm contains 6 ~ 8 leaves, by its running water 15 ~ 30min, after draining away the water, branch inferior end notch is immersed in sterilization 20min in the carbendazim of 0.1%, be inserted in epiphysin solution after taking-up drains away the water, under guaranteeing that otch gos deep into liquid level, blake bottle keeps lucifuge state.Being placed in temperature controls at 18 ~ 25 DEG C, relative moisture 55% ~ 70%, and intensity of illumination is 40 μm of olm
-2s
-1, cultivate under the condition of 16h illumination/8h dark.Change the epiphysin aqueous solution after 7 days once, start to take root, and base of leaf section tomorrow of cultivating in water was taken root after 22 days after 21 days, both quantity of taking root and growing state are substantially identical, illustrate that the epiphysin aqueous solution is taken root without obvious facilitation to base of leaf section water planting tomorrow.
Claims (5)
1. epiphysin is for promoting Radix et Rhizoma Gynurae divaricatae to take root and the application of root development.
2. apply as claimed in claim 1, it is characterized in that, the epiphysin aqueous solution is for promoting Radix et Rhizoma Gynurae divaricatae to take root and root development, and the concentration of the described epiphysin aqueous solution is 50 ~ 200 μm of ol/L.
3. apply as claimed in claim 1 or 2, it is characterized in that, the time that described Radix et Rhizoma Gynurae divaricatae is taken root is 10 ~ 14 days, and the number that described Radix et Rhizoma Gynurae divaricatae is taken root is 11 ~ 18/stem section.
4. apply as claimed in claim 3, it is characterized in that, epiphysin is for promoting Radix et Rhizoma Gynurae divaricatae to take root and the using method of root development comprises:
At temperature 18 ~ 25 DEG C, relative moisture 55% ~ 70%, intensity of illumination 40 μm of olm
-2s
-1and under the condition that replaces of 16h light irradiation time and 8h lucifuge duration, the position of taking root of the annual semi-lignified branch of Radix et Rhizoma Gynurae divaricatae is immersed in the epiphysin aqueous solution that lucifuge places.
5. the application as described in claim 1,2 or 4, is characterized in that, the concentration of the described epiphysin aqueous solution is 50 ~ 200 μm of ol/L.
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Cited By (10)
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| CN107087542A (en) * | 2017-05-16 | 2017-08-25 | 西北农林科技大学 | A kind of apple rootstock B9 rapid propagation methods |
| CN107135946A (en) * | 2017-05-16 | 2017-09-08 | 西北农林科技大学 | A kind of apple rootstock Mac9 rapid propagation methods |
| CN107258538A (en) * | 2017-07-01 | 2017-10-20 | 西北农林科技大学 | A kind of apple rootstock SH38 rapid propagation methods |
| CN107278893A (en) * | 2017-07-01 | 2017-10-24 | 西北农林科技大学 | A kind of apple rootstock SH40 rapid propagation methods |
| CN110506517A (en) * | 2019-07-10 | 2019-11-29 | 广西壮族自治区林业科学研究院 | The method of Southern Pine cuttage seeding hayashishita scale breeding |
| CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
| CN111034616A (en) * | 2019-12-25 | 2020-04-21 | 甘肃农业大学 | Broccoli hairy root high sulforaphane glycoside and sulforaphane synthesis release culture method |
| CN111201956A (en) * | 2020-01-16 | 2020-05-29 | 内蒙古大学 | Application of melatonin in purple perilla cultivation |
| CN114009231A (en) * | 2021-11-23 | 2022-02-08 | 石河子大学 | Fast hard branch cutting seedling raising method for punica granatum with soft seeds |
| CN116267090A (en) * | 2023-05-22 | 2023-06-23 | 云南泽润三七种植有限公司 | Method for improving germination rate of pseudo-ginseng seeds |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107135946A (en) * | 2017-05-16 | 2017-09-08 | 西北农林科技大学 | A kind of apple rootstock Mac9 rapid propagation methods |
| CN107087542A (en) * | 2017-05-16 | 2017-08-25 | 西北农林科技大学 | A kind of apple rootstock B9 rapid propagation methods |
| CN107258538A (en) * | 2017-07-01 | 2017-10-20 | 西北农林科技大学 | A kind of apple rootstock SH38 rapid propagation methods |
| CN107278893A (en) * | 2017-07-01 | 2017-10-24 | 西北农林科技大学 | A kind of apple rootstock SH40 rapid propagation methods |
| CN110506517A (en) * | 2019-07-10 | 2019-11-29 | 广西壮族自治区林业科学研究院 | The method of Southern Pine cuttage seeding hayashishita scale breeding |
| CN110679364B (en) * | 2019-09-27 | 2022-03-25 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
| CN110679364A (en) * | 2019-09-27 | 2020-01-14 | 昆明理工大学 | Method for improving leaf disease resistance of panax notoginseng and application of method |
| CN111034616A (en) * | 2019-12-25 | 2020-04-21 | 甘肃农业大学 | Broccoli hairy root high sulforaphane glycoside and sulforaphane synthesis release culture method |
| CN111034616B (en) * | 2019-12-25 | 2022-05-06 | 甘肃农业大学 | Broccoli hairy root high sulforaphane glycoside and sulforaphane synthesis release culture method |
| CN111201956B (en) * | 2020-01-16 | 2022-01-04 | 内蒙古大学 | Application of melatonin in purple perilla cultivation |
| CN111201956A (en) * | 2020-01-16 | 2020-05-29 | 内蒙古大学 | Application of melatonin in purple perilla cultivation |
| CN114009231A (en) * | 2021-11-23 | 2022-02-08 | 石河子大学 | Fast hard branch cutting seedling raising method for punica granatum with soft seeds |
| CN116267090A (en) * | 2023-05-22 | 2023-06-23 | 云南泽润三七种植有限公司 | Method for improving germination rate of pseudo-ginseng seeds |
| CN116267090B (en) * | 2023-05-22 | 2023-08-01 | 云南泽润三七种植有限公司 | Method for improving germination rate of pseudo-ginseng seeds |
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