CN116369208A - Tissue culture and rapid propagation method for hippeastrum henryi and bulb pretreatment device thereof - Google Patents
Tissue culture and rapid propagation method for hippeastrum henryi and bulb pretreatment device thereof Download PDFInfo
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- CN116369208A CN116369208A CN202310665251.0A CN202310665251A CN116369208A CN 116369208 A CN116369208 A CN 116369208A CN 202310665251 A CN202310665251 A CN 202310665251A CN 116369208 A CN116369208 A CN 116369208A
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- bulb
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- vermilion
- treatment
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- 238000000034 method Methods 0.000 title claims abstract description 29
- 241001466280 Hippeastrum evansiae Species 0.000 title abstract 2
- 230000007246 mechanism Effects 0.000 claims abstract description 68
- 239000001963 growth medium Substances 0.000 claims abstract description 39
- 230000008569 process Effects 0.000 claims abstract description 16
- 230000000694 effects Effects 0.000 claims abstract description 5
- 241000234473 Hippeastrum Species 0.000 claims description 23
- 238000009413 insulation Methods 0.000 claims description 18
- 238000005507 spraying Methods 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 239000012535 impurity Substances 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 229910052956 cinnabar Inorganic materials 0.000 claims 1
- 238000005192 partition Methods 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 239000002609 medium Substances 0.000 description 2
- 241000234270 Amaryllidaceae Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a tissue culture and rapid propagation method for hippeastrum and a bulb pretreatment device thereof, which comprises a pretreatment box body, wherein the internal space of the pretreatment box body is divided into an upper constant temperature treatment area and a lower culture medium treatment area by a baffle mechanism, a placement mechanism is arranged in the middle area of the pretreatment box body in the constant temperature treatment area, an adjusting mechanism is arranged on the inner top surface of the pretreatment box body, and a temperature control module is arranged on the outer side of the adjusting mechanism; a culture medium placing groove is movably connected in the culture medium treatment area of the pretreatment box body. According to the invention, the placing mechanism is arranged in the pretreatment box body, and the vermilion bulbs are sequentially clamped in the placing plate on the support plate when in use, the vermilion bulbs can be clamped by utilizing the V-shaped spring piece in the chuck on the placing plate, so that the stable clamping effect of the vermilion bulbs is finished, meanwhile, the worm motor is started to drive the worm to rotate, and the placing plate can be driven to rotate in the rotating process of the worm, so that the vermilion bulbs can be uniformly heated in the constant temperature treatment through the placing plate.
Description
Technical Field
The invention belongs to the technical field of tissue culture and rapid propagation of hippeastrum, and particularly relates to a tissue culture and rapid propagation method of hippeastrum and a bulb pretreatment device thereof.
Background
The perennial herb of Amaryllidaceae, bulb is nearly spherical and has a grape branch; she Xianlu, band-shaped; the flower stem is hollow, slightly flat, powdery, cylindrical, long and round in split, and has a sharp top end, red color and a purplish color; the flower silk is red, and the anther is in a long round shape; capsule spherical shape; summer in the flowering phase. The hippeastrum is native to mexico, south america and Argentina, and is widely cultivated in various countries; is often grown under forests, lawns, hills or penumbra as ground cover plants. Light preference, slightly negative resistance, dry preference, cool climate, no cold resistance, rainwater and direct sunlight, suitability for growing in sandy soil rich in humus and good in drainage, and no water accumulation; the propagation mode generally adopts sowing propagation or ball-separated propagation.
The existing method for preparing the vermilion bulbs by using the vermilion bulbs as the explant material can directly induce a certain number of bulbs, but in the pretreatment process of the vermilion bulbs, the operation is complex, a corresponding pretreatment device is lacked, and the tissue culture and rapid propagation efficiency of the vermilion bulbs is affected.
Based on the method, a tissue culture and rapid propagation method of hippeastrum and a bulb pretreatment device thereof are provided.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a tissue culture and rapid propagation method for hippeastrum and a bulb pretreatment device thereof for solving the problems in the background art.
In order to solve the technical problems, the invention adopts the following technical scheme: a bulb pretreatment device for tissue culture and rapid propagation of hippeastrum crenata comprises a pretreatment box body, wherein the internal space of the pretreatment box body is divided into an upper constant temperature treatment area and a lower culture medium treatment area by a baffle mechanism;
the device comprises a pretreatment box body, wherein a pretreatment box body is arranged in the middle area of the constant temperature treatment area, the pretreatment box body is provided with a placement mechanism in the middle area of the constant temperature treatment area, the placement mechanism comprises a support plate, placement discs, chucks, worms and an outer plate, the support plate is a rectangular plate with high middle and low two sides, the support plate is slidably connected in the middle of the pretreatment box body, the support plate is rotatably connected with a plurality of placement discs, the chucks are arranged on the placement discs and are used for completing the clamping placement effect of pretreated vermilion bulbs, the outer sides of the placement discs are provided with gears, the inside of the support plate is rotatably connected with the worms, the worms are respectively meshed with the outer sides of the plurality of gears, the support plate is fixedly provided with a worm motor, and the worm motor is used for driving the rotation of the worms;
an adjusting mechanism is arranged on the inner top surface of the pretreatment box body, and a temperature control module is arranged on the outer side of the adjusting mechanism;
the culture medium treatment area of the pretreatment box body is movably connected with a culture medium placing groove, and a culture medium for pretreatment is placed in the culture medium placing groove.
Further, guiding mechanism includes rectangle chamber, lead screw, removes frame, slide, first cylinder and second cylinder, the rectangle chamber is fixed on the interior top surface of pretreatment box the operating window has been seted up to the bottom surface in rectangle chamber, one side rotation in the rectangle chamber is connected with the lead screw, the lead screw motor that is used for driving lead screw pivoted is still installed to the rectangle intracavity, lead screw outside threaded connection has removes the frame, one side is fixed with the gag lever post in the rectangle frame, the opposite side sliding connection of removing the frame is outside the gag lever post, sliding connection has the slide in the middle of removing the frame, the bottom of slide extends to operating window's outside, be fixed with the first cylinder that is used for driving the slide to slide on removing the frame, slide bottom is fixed with the second cylinder, the telescopic shaft bottom of second cylinder is fixed with bulb outside processing mechanism.
Further, bulb outside processing mechanism includes base, processing ring, drive gear and cutter head, the base is fixed in the second cylinder bottom, processing ring rotates to be connected in the base, still rotate in the base and be connected with drive gear, drive gear is through setting up the micro-motor drive in the base, the outside of processing ring is provided with the tooth, the meshing is connected between the tooth in the outside of drive gear and processing ring.
Further, a cutter head is also mounted on the bottom side of the processing ring.
Further, the chuck is a net-shaped chuck, and a V-shaped spring piece is arranged in the chuck.
Further, the side wall of the pretreatment box body is also provided with spraying holes with the same number as the placing discs.
Further, the baffle mechanism includes heat insulating board, hinge and pneumatic cylinder, the heat insulating board is provided with two, and the sum of two heat insulating board cross sectional areas is the same with pretreatment box inner chamber cross sectional area, two the heat insulating board passes through the hinge rotation to be connected with same altitude department in the pretreatment box, every heat insulating board top department still rotates and is connected with two pneumatic cylinders, the other end of pneumatic cylinder rotates to be connected on the lateral wall of pretreatment box.
Further, the heat insulation plate is also provided with a diversion trench.
Further, the side end of the pretreatment box body, which is close to the upper part of the baffle plate mechanism, is also provided with an impurity cleaning port.
A tissue culture and rapid propagation method of hippeastrum based on a bulb pretreatment device for tissue culture and rapid propagation of hippeastrum comprises the following steps:
s1, selecting healthy vermilion bulbs, sequentially clamping the healthy vermilion bulbs in a placing disc on a support plate, clamping the vermilion bulbs by utilizing a V-shaped spring piece in a chuck on the placing disc, and clamping the support plate at the side end of a pretreatment box body by utilizing an outer plate;
s2, starting a temperature control module to perform constant temperature treatment at different temperature stages on a constant temperature treatment area in the pretreatment box body, starting a worm motor to drive a worm to rotate, driving a placing plate to rotate in the rotation process of the worm, and finishing uniform heating of the vermilion bulb during constant temperature treatment through the placing plate;
s3, after the constant temperature treatment is finished, starting an adjusting mechanism, driving a screw rod to rotate by using a screw rod motor, so that a movable frame can be driven to move in a rectangular frame, simultaneously, the first cylinder and the second cylinder can be used for cooperatively matching to finish the movement of the bulb outside treatment mechanism in the bulb outside space, and in the moving and adjusting process of the bulb outside treatment mechanism, the micro motor is used for driving a driving gear to rotate so as to drive a treatment ring to move, so that scales outside the vermilion bulbs can be stripped by the treatment ring, the bulbs on the inner sides are left as explants, and meanwhile, a disinfection solution is sprayed onto the explants by using spraying holes to carry out disinfection treatment on the explants;
s4, the action of pulling up the heat insulation plate is completed by utilizing the contraction of the hydraulic cylinder, the constant temperature treatment area and the culture medium treatment area in the pretreatment box body are communicated, meanwhile, impurities falling onto the baffle plate mechanism are pushed to two sides, then the adjusting mechanism and the bulb outer treatment mechanism are started again, the cutting tool bit arranged under the treatment ring is driven to selectively cut in the outer area of the explant, the cut square blocks directly and naturally fall onto the culture medium in the culture medium placing groove, and the culture medium is cultivated by matching with illumination, so that the pretreatment is completed;
s5, culturing until the bulb grows to grow into seedlings, moving the seedlings to an external culture medium, and performing later rooting culture to obtain vermilion tissue culture seedlings, thereby completing the vermilion tissue culture rapid propagation process.
Compared with the prior art, the invention has the following advantages:
according to the invention, the placing mechanism is arranged in the pretreatment box body, and the vermilion bulbs are sequentially clamped in the placing plate on the support plate when in use, the vermilion bulbs can be clamped by utilizing the V-shaped spring piece in the chuck on the placing plate, so that the stable clamping effect of the vermilion bulbs is finished, meanwhile, the worm motor is started to drive the worm to rotate, and the placing plate can be driven to rotate in the rotating process of the worm, so that the vermilion bulbs can be uniformly heated in the constant temperature treatment through the placing plate.
According to the invention, the screw motor of the adjusting mechanism is arranged in the pretreatment box body to drive the screw to rotate, so that the moving frame can be driven to move in the rectangular frame, meanwhile, the first cylinder and the second cylinder are matched in a cooperative manner, so that the bulb outside treatment mechanism can move in the bulb outside space, in the moving and adjusting process of the bulb outside treatment mechanism, the micro motor is used for driving the driving gear to rotate, so that the treatment ring can be driven to move, scales outside the vermilion bulbs can be stripped through the treatment ring, the bulbs on the inner sides are left as explants, and meanwhile, the disinfection solution is sprayed onto the explants through the spraying holes, so that the disinfection treatment of the explants is convenient and practical.
According to the invention, the baffle mechanism is arranged in the middle of the pretreatment box body, the hydraulic cylinder of the baffle mechanism can be contracted to finish the action of pulling up the heat insulation plate, the constant temperature treatment area inside the pretreatment box body is communicated with the culture medium treatment area, the cutting tool bit arranged below the treatment ring can be utilized to selectively cut in the outer area of the explant, and the cut square blocks directly and naturally fall onto the culture medium in the culture medium placing groove to be matched with illumination for culture of the culture medium, so that the pretreatment is finished.
Drawings
FIG. 1 is a cross-sectional view of the overall structure of the present invention;
FIG. 2 is a cross-sectional view of the placement mechanism of the present invention;
FIG. 3 is a schematic view of the structure of the adjusting mechanism of the present invention;
FIG. 4 is a schematic view of the structure of the baffle mechanism of the present invention;
FIG. 5 is a cross-sectional view of the bulb outer treatment mechanism of the present invention.
Reference numerals illustrate:
1-a treatment box body; 11-a constant temperature treatment zone; 12-a medium treatment zone; 2-a baffle mechanism; 21-a heat insulation plate; 22-hinges; 23-a hydraulic cylinder; 24-diversion trenches; 3-a placement mechanism; 31-supporting plates; 32-placing a tray; 33-a chuck; 34-gear; a 35-V-shaped spring piece; 36-worm; 37-worm motor; 38-an outer plate; 4-an adjusting mechanism; 41-rectangular cavity; 42-an operation window; 43-screw rod; 44-lead screw motor; 45-moving rack; 46-a limit rod; 47-slide; 48-a first cylinder; 49-a second cylinder; 5-bulb outer treatment mechanism; 51-a base; 52-a processing ring; 53-drive gear; 54-cutting heads; 6-spraying holes; 7-Medium placement tank.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
the baffle mechanism 2 comprises two heat insulation plates 21, hinges 22 and hydraulic cylinders 23, wherein the two heat insulation plates 21 are arranged, the sum of the cross-sectional areas of the two heat insulation plates 21 is the same as the cross-sectional area of the inner cavity of the pretreatment box body 1, the two heat insulation plates 21 are rotationally connected to the same height in the pretreatment box body 1 through the hinges, each part above the heat insulation plate 21 is rotationally connected with two hydraulic cylinders 23, and the other ends of the hydraulic cylinders 23 are rotationally connected to the side wall of the pretreatment box body 1.
The heat insulation plate 21 is also provided with a diversion trench 24, so that impurities in the constant temperature treatment area 11 can be prevented from flowing into the culture medium treatment area 12, and separation of the constant temperature treatment area 11 and the culture medium treatment area 12 is completed.
The side end of the pretreatment box body 1 is also provided with an impurity cleaning port close to the upper part of the baffle plate mechanism 2.
The pretreatment box body 1 is provided with a placement mechanism 3 in the middle area in the constant temperature treatment area 11, the placement mechanism 3 comprises a support plate 31, a placement disc 32, a chuck 33, a worm 36 and an outer plate 38, and the support plate 31 is a rectangular plate with high middle and low two sides, so that impurities on the support plate 31 can fall naturally.
The support plate 31 is slidably connected in the middle of the pretreatment box body 1, a plurality of placing discs 32 are rotatably connected to the support plate 31, chucks 33 are arranged on the placing discs 32, the chucks 33 are net-shaped chucks, V-shaped spring pieces 35 are arranged in the chucks 33, and the chucks 33 are used for completing clamping and placing functions of the pretreated vermilion bulbs.
The outside of placing the dish 32 is provided with gear 34, still rotate in the extension board 31 and be connected with worm 36, the outside of worm 36 and a plurality of gears 34 is the meshing respectively and is connected, still be fixed with worm motor 37 on the extension board 31, worm motor 37 is used for driving the rotation of worm 36.
An outer plate 38 is fixed on the outer side of the support plate 31, and the outer plate 38 is clamped at the side end of the pretreatment box body 1, so that the placing mechanism 3 can be taken and used conveniently.
The utility model discloses a pretreatment box, including pretreatment box 1, including the top surface, pretreatment box 1, the top surface is provided with guiding mechanism 4, guiding mechanism 4 includes rectangle chamber 41, lead screw 43, removes frame 45, slide 47, first cylinder 48 and second cylinder 49, rectangle chamber 41 is fixed on the top surface in pretreatment box 1 the operation window 42 has been seted up to the bottom surface in rectangle chamber 41, one side rotates in rectangle chamber 41 and is connected with lead screw 43, still install in rectangle chamber 41 and be used for driving lead screw 43 pivoted lead screw motor 44, lead screw 43 outside threaded connection has removes frame 45, one side is fixed with gag lever post 46 in the rectangle frame 41, the opposite side sliding connection of removing frame 45 is outside gag lever post 46, sliding connection has slide 47 in the middle of removing frame 45, the bottom of slide 47 extends to the outside of operation window 42, be fixed with on the removal frame 45 and be used for driving slide 47 gliding first cylinder 48, the slide 47 bottom is fixed with second cylinder 49, the flexible axle bottom end of second cylinder 49 is fixed with scale outside processing mechanism 5.
The bulb outside treatment mechanism 5 comprises a base 51, a treatment ring 52 and a driving gear 53, wherein the base 51 is fixed at the bottom end of the second air cylinder 49, the treatment ring 52 is rotationally connected in the base 51, the driving gear 53 is driven by a micro motor arranged in the base 51, teeth are arranged on the outer side of the treatment ring 52, and the driving gear 53 is in meshed connection with the teeth on the outer side of the treatment ring 52.
The bottom side of the treatment ring 52 is also provided with a cutting head 54, which cutting head 54 is used for cutting the desired part of the vermilion bulb.
The outside of the adjusting mechanism 4 is provided with a temperature control module, the temperature control module is the existing temperature control equipment, and the staged constant temperature treatment process of different temperatures in the pretreatment box body 1 can be completed through the temperature control module.
A culture medium placing groove 7 is movably connected in a culture medium treatment area 12 of the pretreatment box body 1, and a culture medium for pretreatment is placed in the culture medium placing groove 7.
The side wall of the pretreatment box body 1 is also provided with spraying holes 6 with the same quantity as the placing plates 32, and the spraying holes 6 are used for spraying disinfection operation of disinfectant.
Example 2, a method for tissue culture and rapid propagation of hippeastrum based on the bulb pretreatment device for tissue culture and rapid propagation of hippeastrum in example 1, comprises the following steps:
s1, selecting healthy vermilion bulbs, sequentially clamping the healthy vermilion bulbs in a placing disc 32 on a support plate 31, clamping the vermilion bulbs by utilizing a V-shaped spring piece 34 in a chuck 33 on the placing disc 32, and then clamping the support plate 31 at the side end of a pretreatment box body 1 by utilizing an outer plate 38;
s2, starting a temperature control module to perform constant temperature treatment of different temperature stages on the constant temperature treatment area 11 in the pretreatment box body 1, starting a worm motor 37 to drive a worm 36 to rotate, and driving a placing plate 32 to rotate in the rotation process of the worm 36, wherein the placing plate 32 can be used for completing uniform heating of the vermilion bulbs during constant temperature treatment;
s3, after the constant temperature treatment is finished, starting an adjusting mechanism 4, driving a screw 43 to rotate by using a screw motor 44, so that a movable frame 45 can be driven to move in a rectangular frame 41, simultaneously, the first air cylinder 48 and the second air cylinder 49 can be used for cooperatively matching to finish the movement of the bulb outside treatment mechanism 5 in a bulb outside space, and in the process of moving and adjusting the bulb outside treatment mechanism 5, a miniature motor is used for driving a driving gear 53 to rotate so as to drive a treatment ring 52 to move, so that scales outside the vermilion bulbs can be stripped by the treatment ring 52, the bulbs on the inner sides are left as explants, and meanwhile, a disinfection solution is sprayed onto the explants by using a spraying hole 6 to carry out disinfection treatment of the explants;
s4, the hydraulic cylinder 23 is used for shrinking to finish the pulling-up effect of the heat insulation plate 21, the constant temperature treatment area 11 and the culture medium treatment area 12 in the pretreatment box body 1 are communicated, impurities falling onto the baffle plate mechanism 2 are pushed to two sides at the same time, then the adjusting mechanism 4 and the bulb outside treatment mechanism 5 are started again, the cutter head 54 arranged under the treatment ring 52 is driven to selectively cut in the outer area of the explant, the cut square blocks directly and naturally fall onto the culture medium in the culture medium placing groove 7, and the culture medium is cultivated by matching with illumination, so that the pretreatment is finished;
s5, culturing until the bulb grows to grow into seedlings, moving the seedlings to an external culture medium, and performing later rooting culture to obtain vermilion tissue culture seedlings, thereby completing the vermilion tissue culture rapid propagation process.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The bulb pretreatment device for tissue culture and rapid propagation of hippeastrum crenata is characterized in that: the pretreatment device comprises a pretreatment box body (1), wherein the internal space of the pretreatment box body (1) is divided into an upper constant temperature treatment area (11) and a lower culture medium treatment area (12) by a partition plate mechanism (2);
the pretreatment box body (1) is provided with a placement mechanism (3) in the middle area in the constant temperature treatment area (11), the placement mechanism (3) comprises a support plate (31), a placement disc (32), a chuck (33), a worm (36) and an outer plate (38), the support plate (31) is a rectangular plate with high middle and low two sides, the support plate (31) is slidably connected in the middle of the pretreatment box body (1), a plurality of placement discs (32) are rotatably connected on the support plate (31), a chuck (33) is arranged on the placement disc (32), the chuck (33) is used for completing the clamping placement effect of the pretreatment of the vermilion bulbs, a gear (34) is arranged on the outer side of the placement disc (32), the worm (36) is rotatably connected in the support plate (31), the worm (36) is respectively meshed and connected with the outer sides of the plurality of gears (34), a worm motor (37) is fixedly arranged on the support plate (31), the worm motor (37) is used for driving the rotation of the worm (36), and the outer plate (38) is fixedly connected with the outer plate (1) at the outer plate (38);
an adjusting mechanism (4) is arranged on the inner top surface of the pretreatment box body (1), and a temperature control module is arranged on the outer side of the adjusting mechanism (4);
a culture medium placing groove (7) is movably connected in a culture medium treatment area (12) of the pretreatment box body (1), and a culture medium for pretreatment is placed in the culture medium placing groove (7).
2. The bulb pretreatment device for tissue culture and rapid propagation of hippeastrum of claim 1, wherein the adjustment mechanism (4) comprises a rectangular cavity (41), a screw rod (43), a movable frame (45), a sliding seat (47), a first air cylinder (48) and a second air cylinder (49), the rectangular cavity (41) is fixed on the inner top surface of the pretreatment box body (1), an operation window (42) is formed in the bottom surface of the rectangular cavity (41), the screw rod (43) is rotatably connected to one side in the rectangular cavity (41), a screw rod motor (44) for driving the screw rod (43) to rotate is further installed in the rectangular cavity (41), a movable frame (45) is in threaded connection with the outer side of the screw rod (43), a limit rod (46) is fixed to one side in the rectangular frame (41), the other side of the movable frame (45) is in sliding connection with the limit rod (46), the sliding seat (47) is in sliding connection with the middle of the movable frame (45), the bottom end of the sliding seat (47) extends to the outer side of the operation window (42), the movable frame (45) is also provided with a first air cylinder (47) for driving the bottom end of the sliding seat (47), and a bulb outside treatment mechanism (5) is fixed at the bottom end of the telescopic shaft of the second air cylinder (49).
3. The bulb pretreatment device for tissue culture and rapid propagation of hippeastrum of claim 2, wherein the bulb outside treatment mechanism (5) comprises a base (51), a treatment ring (52) and a driving gear (53), the base (51) is fixed at the bottom end of the second cylinder (49), the treatment ring (52) is rotationally connected in the base (51), the driving gear (53) is driven by a micro motor arranged in the base (51), teeth are arranged on the outer side of the treatment ring (52), and the driving gear (53) is in meshed connection with the teeth on the outer side of the treatment ring (52).
4. A bulb pretreatment device for tissue culture and rapid propagation of hippeastrum henryi as claimed in claim 3, characterized in that a cutter head (54) is further mounted on the bottom side of the treatment ring (52).
5. Bulb pretreatment device for tissue culture and rapid propagation of hippeastrum henryi according to claim 1, characterized in that the chuck (33) is a net-shaped chuck, and a V-shaped spring piece (35) is arranged in the chuck (33).
6. Bulb pretreatment device for tissue culture and rapid propagation of hippeastrum of vermilion according to claim 1, characterized in that the side wall of the pretreatment box body (1) is also provided with spray holes (6) the same as the placing plates (32).
7. The bulb pretreatment device for tissue culture and rapid propagation of hippeastrum of vermilion according to claim 1, wherein the baffle mechanism (2) comprises two heat insulation boards (21), a hinge (22) and a hydraulic cylinder (23), the two heat insulation boards (21) are arranged, the sum of the cross-sectional areas of the two heat insulation boards (21) is identical to the cross-sectional area of the inner cavity of the pretreatment box body (1), the two heat insulation boards (21) are rotationally connected at the same height in the pretreatment box body (1) through the hinge, two hydraulic cylinders (23) are rotationally connected to the upper portion of each heat insulation board (21), and the other end of each hydraulic cylinder (23) is rotationally connected to the side wall of the pretreatment box body (1).
8. The bulb pretreatment device for tissue culture and rapid propagation of hippeastrum crenata according to claim 7, wherein the heat insulation plate (21) is further provided with a diversion trench (24).
9. The bulb pretreatment device for tissue culture and rapid propagation of hippeastrum of cinnabar as claimed in claim 7, wherein an impurity cleaning port is further arranged at the side end of the pretreatment box body (1) above the baffle mechanism (2).
10. The tissue culture and rapid propagation method of the hippeastrum based on the bulb pretreatment device for tissue culture and rapid propagation of the hippeastrum is characterized by comprising the following steps of:
s1, selecting healthy vermilion bulbs, sequentially clamping the healthy vermilion bulbs in a placing disc (32) on a support plate (31), clamping the vermilion bulbs by using V-shaped spring pieces (35) in a chuck (33) on the placing disc (32), and then clamping the support plate (31) at the side end of a pretreatment box body (1) by using an outer plate (38);
s2, starting a temperature control module to perform constant temperature treatment on a constant temperature treatment area (11) in the pretreatment box body (1) at different temperature stages, starting a worm motor (37) to drive a worm (36) to rotate, and driving a placing disc (32) to rotate in the rotating process of the worm (36), wherein the placing disc (32) can be used for completing uniform heating of the vermilion bulbs during constant temperature treatment;
s3, after the constant temperature treatment is finished, starting an adjusting mechanism (4), driving a screw (43) to rotate by using a screw motor (44), driving a movable frame (45) to move in a rectangular frame (41), simultaneously completing the movement of a bulb outside treatment mechanism (5) in a bulb outside space by using the cooperation of a first air cylinder (48) and a second air cylinder (49), driving a driving gear (53) to rotate by using a micro motor in the movement adjusting process of the bulb outside treatment mechanism (5), driving a treatment ring (52) to move, thereby stripping scales outside the vermilion bulbs by using the treatment ring (52), leaving the bulbs on the inner sides as explants, and spraying a disinfection solution onto the explants by using a spraying hole (6) to carry out disinfection treatment of the explants;
s4, the action of pulling up the heat insulation plate (21) is completed by means of shrinkage of the hydraulic cylinder (23), the constant temperature treatment area (11) and the culture medium treatment area (12) in the pretreatment box body (1) are communicated, meanwhile, impurities falling onto the baffle mechanism (2) are pushed to two sides, then the adjusting mechanism (4) and the bulb outside treatment mechanism (5) are started again, the cutting tool bit (54) arranged below the treatment ring (52) is driven to selectively cut in the outer area of the explant, and the cut square blocks directly and naturally fall into the culture medium in the culture medium placing groove (7) to be matched with illumination for culture of the culture medium, so that pretreatment is completed;
s5, culturing until the bulb grows to grow into seedlings, moving the seedlings to an external culture medium, and performing later rooting culture to obtain vermilion tissue culture seedlings, thereby completing the vermilion tissue culture rapid propagation process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310665251.0A CN116369208B (en) | 2023-06-07 | 2023-06-07 | Tissue culture and rapid propagation method for hippeastrum henryi and bulb pretreatment device thereof |
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002273693A (en) * | 2001-03-19 | 2002-09-25 | Japan Tobacco Inc | Bulb cutting machine |
| CN104221853A (en) * | 2014-05-26 | 2014-12-24 | 中国长江三峡集团公司 | Rapid propagation method capable of inducing hippeastrum bulbs by temperature fluctuation |
| CN106106174A (en) * | 2016-07-29 | 2016-11-16 | 广东圣茵花卉园艺有限公司 | A kind of propagation method of hippeastrum bulb section |
| CN109220791A (en) * | 2018-09-12 | 2019-01-18 | 上海市农业科学院 | A kind of tissue culture method using bulb breeding Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait |
| CN110447537A (en) * | 2019-08-23 | 2019-11-15 | 江苏省中国科学院植物研究所 | A kind of tissue culture method obtaining regeneration plant using hippeastrum bulb disk as explant |
| CN111713207A (en) * | 2020-07-14 | 2020-09-29 | 杭州植物园(杭州西湖园林科学研究院) | Automatic cutting device for lycoris radiata bulb cuttage |
| CN217136353U (en) * | 2022-05-05 | 2022-08-09 | 湖南九穗农业有限公司 | Strawberry is grown seedlings with detoxification treatment facility |
| CN217318168U (en) * | 2022-04-12 | 2022-08-30 | 邢台市农业科学研究院 | Quick slicer of hippeastrum flake |
| CN217336807U (en) * | 2022-06-01 | 2022-09-02 | 邢台市农业科学研究院 | Automatic watering device for hippeastrum rutilum planting |
| CN217336369U (en) * | 2022-06-09 | 2022-09-02 | 邢台市农业科学研究院 | Seed ball disinfection device for hippeastrum rutilum planting |
| CN116210590A (en) * | 2023-02-28 | 2023-06-06 | 河南德道农业科技有限公司 | Potato plant tissue culture seedling cutting propagation transfer device and transfer method thereof |
-
2023
- 2023-06-07 CN CN202310665251.0A patent/CN116369208B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002273693A (en) * | 2001-03-19 | 2002-09-25 | Japan Tobacco Inc | Bulb cutting machine |
| CN104221853A (en) * | 2014-05-26 | 2014-12-24 | 中国长江三峡集团公司 | Rapid propagation method capable of inducing hippeastrum bulbs by temperature fluctuation |
| CN106106174A (en) * | 2016-07-29 | 2016-11-16 | 广东圣茵花卉园艺有限公司 | A kind of propagation method of hippeastrum bulb section |
| CN109220791A (en) * | 2018-09-12 | 2019-01-18 | 上海市农业科学院 | A kind of tissue culture method using bulb breeding Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait |
| CN110447537A (en) * | 2019-08-23 | 2019-11-15 | 江苏省中国科学院植物研究所 | A kind of tissue culture method obtaining regeneration plant using hippeastrum bulb disk as explant |
| CN111713207A (en) * | 2020-07-14 | 2020-09-29 | 杭州植物园(杭州西湖园林科学研究院) | Automatic cutting device for lycoris radiata bulb cuttage |
| CN217318168U (en) * | 2022-04-12 | 2022-08-30 | 邢台市农业科学研究院 | Quick slicer of hippeastrum flake |
| CN217136353U (en) * | 2022-05-05 | 2022-08-09 | 湖南九穗农业有限公司 | Strawberry is grown seedlings with detoxification treatment facility |
| CN217336807U (en) * | 2022-06-01 | 2022-09-02 | 邢台市农业科学研究院 | Automatic watering device for hippeastrum rutilum planting |
| CN217336369U (en) * | 2022-06-09 | 2022-09-02 | 邢台市农业科学研究院 | Seed ball disinfection device for hippeastrum rutilum planting |
| CN116210590A (en) * | 2023-02-28 | 2023-06-06 | 河南德道农业科技有限公司 | Potato plant tissue culture seedling cutting propagation transfer device and transfer method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 邹水平;郭翔;袁嘉铭;吴小业;: "朱顶红组培苗小鳞茎切割诱导新植株技术研究", 湖南农业科学, no. 04 * |
| 邹水平;郭翔;袁嘉铭;吴小业;: "朱顶红组培苗小鳞茎切割诱导新植株技术研究", 湖南农业科学, no. 04, 27 April 2018 (2018-04-27), pages 5 - 7 * |
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