CN104221853A - Rapid propagation method capable of inducing hippeastrum bulbs by temperature fluctuation - Google Patents
Rapid propagation method capable of inducing hippeastrum bulbs by temperature fluctuation Download PDFInfo
- Publication number
- CN104221853A CN104221853A CN201410225722.7A CN201410225722A CN104221853A CN 104221853 A CN104221853 A CN 104221853A CN 201410225722 A CN201410225722 A CN 201410225722A CN 104221853 A CN104221853 A CN 104221853A
- Authority
- CN
- China
- Prior art keywords
- bulb
- explant
- illumination
- bulbs
- herb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a rapid propagation method capable of inducing hippeastrum bulbs by temperature fluctuation. The method comprises the following steps: (1) processing bulbs by temperature fluctuation; (2) selecting an explant; (3) starting culture, namely cutting the explant obtained in the step (2) to obtain a square block of 1 square meter, inoculating the square block to a start culture medium, culturing for 15 days till the incision of the explant turns dark red and raised bulbs on a bulb disc are generated, and continuously culturing for 25 days, so that the bulbs are enlarged to come into leaf and grow up seedlings; (4) performing bulb subculture multiplication, and culturing for 25 days to grow single seedlings with bulbs; and (5) performing rooting culture and transplanting, namely transplanting the single seedlings with bulbs cultured in the step (4) into a rooting medium, and transplanting the seedlings rooting within 15 days onto a matrix, thereby culturing to obtain hippeastrum tissue culture seedlings. According to the method provided by the invention, the defects such as slow bulb culture start, low multiplication rate, long rooting period and low transplanting survival rate of conventional culture tissue technology can be overcome, and the aim of growing seedlings in a factory on a large scale is achieved.
Description
Technical field
The present invention relates to the method for quickly breeding of a Plants, especially a kind of quick-breeding method of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb.
Background technology
Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait (Hippeastrum) through-stone garlic amaryllis belongs to perennial evergreen herbaceous plant, and kind series can be used for potted plant, this kind series polyphyll, petal is circular, and Hua great, blooms in early summer, the four seasons all can bloom, and are rare potted plant and flower bed material arranged, expensive.
" Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait somatic embryo occurs and plant regeneration research " that Gong Xue qin 2012 is delivered at " gardening journal " and imperial " foundation of hippeastrum bulb bud inducement and plant regeneration system " delivered at " northwest Botany Gazette " for 2007 of Liu Qun are all do explant material with bulb, though the bulb of some directly can be induced, but there is the problems such as reproduction coefficient is low, the production cycle is long.In the research of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait tissue-culturing rapid propagation, there is at present induction bulb start the deficiencies such as slow, growth rate is low, the cycle of taking root is long and transplant survival is low.
Summary of the invention
Technical problem to be solved by this invention is to provide the quick-breeding method of a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb, bulb induce can be overcome in existing tissue culture technology and starts the shortcomings such as slow, growth rate is low, the cycle of taking root is long and transplant survival is low, reduce workload and reduce production cost, reaching the object of batch production scale seedling raising.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of quick-breeding method of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb, and the method comprises the following steps:
1) bulb intermittent warming: hippeastrum bulb disease-free for stalwartness is first placed in climatic chamber 25 ~ 30 DEG C process every day, then be placed in 7 ~ 10 DEG C of refrigerators and process, for subsequent use after processing 12 ~ 18 days continuously;
2) explant is chosen: by through step 1) hippeastrum bulb of intermittent warming peels off 1 ~ 3 layer of scale of outside, and getting inner scale is explant, sterilization;
3) Primary culture: by step 2) obtain explant cutting 1cm
2on square access Primary culture base, illumination box in temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx is cultivated, from the protruding bulb of the red generation plateau of explant incision colour-darkening after 15d, after continuing to cultivate 25d, bulb increases the seedling that comes into leaves;
4) bulb shoot proliferation: until step 3) seedling of bulb differentiation cultivated is when growing to 3 ~ 5cm, segmentation is transferred on Primary culture base again, under temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx condition, carry out bulb Multiplying culture, after cultivating 25d, grow up to the single seedling with bulb;
5) culture of rootage is transplanted: by step 4) the single seedling with bulb cultivated is transferred in root media, cultivate under temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx condition, take root after 15 days, then transplant in matrix, be namely trained Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait plantlet in vitro.
Further, step 1) in, the time that hippeastrum bulb is placed in climatic chamber every day is 12 hours, and the time being placed in refrigerator is 12 hours.
Further, step 2) sterilization method be: first explant is placed in mass concentration be 5% washing powder solution soak 10min, be placed in running water 1h again, first with the alcohol-pickled 30s that mass concentration is 75% on superclean bench, again with 0.1% mercuric chloride sterilization 25min, after aseptic water washing 5 times, explant is placed on aseptic filter paper and blots excessive moisture, the explant after being sterilized.
Further, step 3) and step 4) Primary culture base used is: MS minimal medium+6-BA2.0mg/L+NAA2.0mg/L+ lactoalbumin hydrolysate 0.1g/L+ sucrose 30g/L+ agar powder 6g/L.
Further, step 5) root media used is: 1/2MS minimal medium+IBA0.5mg/L; Matrix used is: ash: mass ratio humous is 1:2.
The quick-breeding method of a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb provided by the invention, beneficial effect is as follows:
1, to overcome in existing tissue culture technology bulb induce by intermittent warming hippeastrum bulb explant and start the shortcomings such as slow, growth rate is low, the cycle of taking root is long and transplant survival is low, reduce workload and reduce production cost, reaching the object of batch production scale seedling raising;
2, hippeastrum bulb becomes large, startup is fast, growth rate is fast, the cycle of taking root is short and survive transplanting survival rate advantages of higher significantly too conventional treatment.Intermittent warming and conventional treatment result as shown in table 1 below:
Table 1: alternating temperature and conventional treatment form contrast (average data contrasts)
From in upper table: |
Bulb mean fresh through intermittent warming reaches 1.47g/, and increase 0.91g/ than conventional treatment mean fresh, gaining effect is remarkable.
Bulb shifts to an earlier date 5d by intermittent warming than conventional treatment start-up time, substantially reduces hippeastrum bulb induction time.
It is fast that bulb starts growth rate by intermittent warming than conventional treatment, shortens 10d than conventional speeds.
Bulb is shorter than the conventional treatment root induction cycle by intermittent warming, shortens 5d than conventional induction time.
Bulb by intermittent warming than conventional treatment transplanting survival rate mean height 23%.
Reduce workload and reduce production cost, reaching the object of batch production scale seedling raising.
Embodiment
Embodiment one
A quick-breeding method for Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb, the method comprises the following steps:
1) bulb intermittent warming: hippeastrum bulb disease-free for stalwartness is first placed in climatic chamber 25 DEG C process every day, then be placed in 7 DEG C of refrigerators and process, process is for subsequent use after 12 days continuously;
2) explant is chosen: by through step 1) hippeastrum bulb of intermittent warming peels off 1 ~ 3 layer of scale of outside, and getting inner scale is explant, sterilization;
3) Primary culture: by step 2) obtain explant cutting 1cm
2on square access Primary culture base, illumination box in temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx is cultivated, from the protruding bulb of the red generation plateau of explant incision colour-darkening after 15d, after continuing to cultivate 25d, bulb increases the seedling that comes into leaves;
4) bulb shoot proliferation: until step 3) seedling of bulb differentiation cultivated is when growing to 3 ~ 5cm, segmentation is transferred on Primary culture base again, under temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx condition, carry out bulb Multiplying culture, after cultivating 25d, grow up to the single seedling with bulb;
5) culture of rootage is transplanted: by step 4) the single seedling with bulb cultivated is transferred in root media, cultivate under temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx condition, take root after 15 days, then transplant in matrix, be namely trained Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait plantlet in vitro.
Step 1) in, the time that hippeastrum bulb is placed in climatic chamber every day is 12 hours, and the time being placed in refrigerator is 12 hours.
Step 2) sterilization method be: first explant is placed in mass concentration be 5% washing powder solution soak 10min, be placed in running water 1h again, first with the alcohol-pickled 30s that mass concentration is 75% on superclean bench, again with 0.1% mercuric chloride sterilization 25min, after aseptic water washing 5 times, explant is placed on aseptic filter paper and blots excessive moisture, the explant after being sterilized.
Step 3) and step 4) Primary culture base used is: MS minimal medium+6-BA2.0mg/L+NAA2.0mg/L+ lactoalbumin hydrolysate 0.1g/L+ sucrose 30g/L+ agar powder 6g/L.
Step 5) root media used is: 1/2MS minimal medium+IBA0.5mg/L; Matrix used is: ash: mass ratio humous is 1:2.
Embodiment two
A quick-breeding method for Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb, the method comprises the following steps:
1) bulb intermittent warming: hippeastrum bulb disease-free for stalwartness is first placed in climatic chamber 30 DEG C process every day, then be placed in 10 DEG C of refrigerators and process, process is for subsequent use after 18 days continuously;
2) explant is chosen: by through step 1) hippeastrum bulb of intermittent warming peels off 1 ~ 3 layer of scale of outside, and getting inner scale is explant, sterilization;
3) Primary culture: by step 2) obtain explant cutting 1cm
2on square access Primary culture base, illumination box in temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx is cultivated, from the protruding bulb of the red generation plateau of explant incision colour-darkening after 15d, after continuing to cultivate 25d, bulb increases the seedling that comes into leaves;
4) bulb shoot proliferation: until step 3) seedling of bulb differentiation cultivated is when growing to 3 ~ 5cm, segmentation is transferred on Primary culture base again, under the condition of temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx, carry out bulb Multiplying culture, after cultivating 25d, grow up to the single seedling with bulb;
5) culture of rootage is transplanted: by step 4) the single seedling with bulb cultivated is transferred in root media, (cultivate under the condition of temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx, take root after 15 days, then transplant in matrix, be namely trained Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait plantlet in vitro.
Step 1) in, the time that hippeastrum bulb is placed in climatic chamber every day is 12 hours, and the time being placed in refrigerator is 12 hours.
Step 2) sterilization method be: first explant is placed in mass concentration be 5% washing powder solution soak 10min, be placed in running water 1h again, first with the alcohol-pickled 30s that mass concentration is 75% on superclean bench, again with 0.1% mercuric chloride sterilization 25min, after aseptic water washing 5 times, explant is placed on aseptic filter paper and blots excessive moisture, the explant after being sterilized.
Step 3) and step 4) Primary culture base used is: MS minimal medium+6-BA2.0mg/L+NAA2.0mg/L+ lactoalbumin hydrolysate 0.1g/L+ sucrose 30g/L+ agar powder 6g/L.
Step 5) root media used is: 1/2MS minimal medium+IBA0.5mg/L; Matrix used is: ash: mass ratio humous is 1:2.
Embodiment three
A quick-breeding method for Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb, the method comprises the following steps:
1) bulb intermittent warming: hippeastrum bulb disease-free for stalwartness is first placed in climatic chamber 28 DEG C process every day, then be placed in 8 DEG C of refrigerators and process, process is for subsequent use after 15 days continuously;
2) explant is chosen: by through step 1) hippeastrum bulb of intermittent warming peels off 1 ~ 3 layer of scale of outside, and getting inner scale is explant, sterilization;
3) Primary culture: by step 2) obtain explant cutting 1cm
2on square access Primary culture base, illumination box in temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx is cultivated, from the protruding bulb of the red generation plateau of explant incision colour-darkening after 15d, after continuing to cultivate 25d, bulb increases the seedling that comes into leaves;
4) bulb shoot proliferation: until step 3) seedling of bulb differentiation cultivated is when growing to 3 ~ 5cm, segmentation is transferred on Primary culture base again, under the condition of temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx, carry out bulb Multiplying culture, after cultivating 25d, grow up to the single seedling with bulb;
5) culture of rootage is transplanted: by step 4) the single seedling with bulb cultivated is transferred in root media, cultivate under the condition of temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx, take root after 15 days, then transplant in matrix, be namely trained Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait plantlet in vitro.
Step 1) in, the time that hippeastrum bulb is placed in climatic chamber every day is 12 hours, and the time being placed in refrigerator is 12 hours.
Step 2) sterilization method be: first explant is placed in mass concentration be 5% washing powder solution soak 10min, be placed in running water 1h again, first with the alcohol-pickled 30s that mass concentration is 75% on superclean bench, again with 0.1% mercuric chloride sterilization 25min, after aseptic water washing 5 times, explant is placed on aseptic filter paper and blots excessive moisture, the explant after being sterilized.
Step 3) and step 4) Primary culture base used is: MS minimal medium+6-BA2.0mg/L+NAA2.0mg/L+ lactoalbumin hydrolysate 0.1g/L+ sucrose 30g/L+ agar powder 6g/L.
Step 5) root media used is: 1/2MS minimal medium+IBA0.5mg/L; Matrix used is: ash: mass ratio humous is 1:2.
Claims (6)
1. a quick-breeding method for Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb, is characterized in that the method comprises the following steps:
1) bulb intermittent warming: hippeastrum bulb disease-free for stalwartness is first placed in climatic chamber 25 ~ 30 DEG C process every day, then be placed in 7 ~ 10 DEG C of refrigerators and process, for subsequent use after processing 12 ~ 18 days continuously;
2) choose explant: 1 ~ 3 layer of scale hippeastrum bulb through step 1) intermittent warming being peelled off outside, getting inner scale is explant, sterilization;
3) Primary culture: by step 2) obtain explant cutting 1cm
2on square access Primary culture base, illumination box in temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx is cultivated, from the protruding bulb of the red generation plateau of explant incision colour-darkening after 15d, after continuing to cultivate 25d, bulb increases the seedling that comes into leaves;
4) bulb shoot proliferation: when the seedling of the bulb differentiation that step 3) is cultivated grows to 3 ~ 5cm, segmentation is transferred on Primary culture base again, under temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx condition, carry out bulb Multiplying culture, after cultivating 25d, grow up to the single seedling with bulb;
5) culture of rootage is transplanted: be transferred in root media by the single seedling with bulb that step 4) is cultivated, cultivate under temperature 25 ± 1 DEG C, humidity 75 ± 2%, illumination 16h/d, intensity of illumination 1800 ~ 2000lx condition, take root after 15 days, then transplant in matrix, be namely trained Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait plantlet in vitro.
2. the method for a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb according to claim 1, it is characterized in that: in step 1), the time that hippeastrum bulb is placed in climatic chamber every day is 12 hours, and the time being placed in refrigerator is 12 hours.
3. the method for a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb according to claim 1, it is characterized in that step 2) sterilization method be: first explant is placed in mass concentration be 5% washing powder solution soak 10min, be placed in running water 1h again, first with the alcohol-pickled 30s that mass concentration is 75% on superclean bench, again with 0.1% mercuric chloride sterilization 25min, after aseptic water washing 5 times, explant is placed on aseptic filter paper and blots excessive moisture, the explant after being sterilized.
4. the method for a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb according to claim 1, is characterized in that step 3) and step 4) Primary culture base used are: MS minimal medium+6-BA2.0 mg/L+NAA2.0 mg/L+ lactoalbumin hydrolysate 0.1g/L+sucrose 30g/L+agar powder 6g/L.
5. the method for a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb according to claim 1, is characterized in that step 5) root media used is: 1/2MS minimal medium+IBA0.5 mg/L.
6. the method for a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb according to claim 1, is characterized in that step 5) matrix used is: ash: mass ratio humous is 1:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410225722.7A CN104221853B (en) | 2014-05-26 | 2014-05-26 | A kind of quick-breeding method of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410225722.7A CN104221853B (en) | 2014-05-26 | 2014-05-26 | A kind of quick-breeding method of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104221853A true CN104221853A (en) | 2014-12-24 |
| CN104221853B CN104221853B (en) | 2016-03-23 |
Family
ID=52211475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410225722.7A Active CN104221853B (en) | 2014-05-26 | 2014-05-26 | A kind of quick-breeding method of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104221853B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686330A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for hippeastrum vittatum |
| CN107318655A (en) * | 2017-08-11 | 2017-11-07 | 云南青谷生物科技有限公司 | A kind of bulbus fritillariae cirrhosae seedling fostering method |
| CN109220791A (en) * | 2018-09-12 | 2019-01-18 | 上海市农业科学院 | A kind of tissue culture method using bulb breeding Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait |
| CN110741901A (en) * | 2019-11-13 | 2020-02-04 | 云南省农业科学院花卉研究所 | Cultivation method of heavy valve hippeastrum rutilum |
| CN116369208A (en) * | 2023-06-07 | 2023-07-04 | 包头市农牧科学技术研究所 | Tissue culture and rapid propagation method for hippeastrum henryi and bulb pretreatment device thereof |
-
2014
- 2014-05-26 CN CN201410225722.7A patent/CN104221853B/en active Active
Non-Patent Citations (4)
| Title |
|---|
| 刘群龙等: "朱顶红鳞茎芽诱导及植株再生体系的建立", 《西北植物学报》, vol. 27, no. 12, 31 December 2007 (2007-12-31), pages 2551 - 2554 * |
| 张亚玲等: "朱顶红组织培养最佳繁殖途径的研究", 《中国园艺学会第七届青年学术讨论会论文集》, 1 July 2006 (2006-07-01), pages 628 - 636 * |
| 沈苗苗等: "朱顶红组织培养研究进展", 《黑龙江农业科学》, no. 10, 31 December 2011 (2011-12-31), pages 135 - 138 * |
| 郑一强等: "东方百合试管鳞茎形成条件优化", 《中国农学通报》, vol. 27, no. 6, 31 December 2011 (2011-12-31), pages 90 - 94 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686330A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for hippeastrum vittatum |
| CN107318655A (en) * | 2017-08-11 | 2017-11-07 | 云南青谷生物科技有限公司 | A kind of bulbus fritillariae cirrhosae seedling fostering method |
| CN109220791A (en) * | 2018-09-12 | 2019-01-18 | 上海市农业科学院 | A kind of tissue culture method using bulb breeding Hipeastrum vittalum (L Her.) Herb.- Amaryllisvittata Ait |
| CN109220791B (en) * | 2018-09-12 | 2021-10-08 | 上海市农业科学院 | Tissue culture method for breeding hippeastrum rutilum by using bulbs |
| CN110741901A (en) * | 2019-11-13 | 2020-02-04 | 云南省农业科学院花卉研究所 | Cultivation method of heavy valve hippeastrum rutilum |
| CN110741901B (en) * | 2019-11-13 | 2021-09-28 | 云南省农业科学院花卉研究所 | Cultivation method of heavy valve hippeastrum rutilum |
| CN116369208A (en) * | 2023-06-07 | 2023-07-04 | 包头市农牧科学技术研究所 | Tissue culture and rapid propagation method for hippeastrum henryi and bulb pretreatment device thereof |
| CN116369208B (en) * | 2023-06-07 | 2024-04-26 | 包头市农牧科学技术研究所 | Tissue culture and rapid propagation method for hippeastrum henryi and bulb pretreatment device thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104221853B (en) | 2016-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103371100B (en) | Tissue culture and rapid propagation method of nobile-type dendrobium seedlings | |
| CN106171998B (en) | A method of induction Cremastra appendiculata protocorm stem eye cluster proliferation | |
| CN103931497B (en) | A kind of method improving dragon fruit plantlet in vitro planting percent | |
| CN104221853B (en) | A kind of quick-breeding method of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait intermittent warming induction bulb | |
| CN102499080B (en) | Plant fast propagating method using fagopyrum tataricum leaf stalks as explants | |
| CN103202229B (en) | Tissue culturing and rapid propagating method for chloranthy florida var. plena | |
| CN108901856B (en) | method for efficient somatic embryogenesis and plant regeneration of camellia plants | |
| CN103444552A (en) | Method for inducing eggplant anther to regenerate haplobiont | |
| CN104041412A (en) | Rapid propagation method for tissue culture of Guizhou hemiboea cavaleriei | |
| CN103651137A (en) | Rhynchostylis protocorm rapid breeding method | |
| CN101836587B (en) | Method for obtaining eustoma regeneration plant by anther culture | |
| CN103004604B (en) | Breeding method for vanda | |
| CN102907326B (en) | Tissue culture propagation method for Medicagao Sativa L. | |
| CN103190344A (en) | Tissue culture method of fargesii | |
| CN105475129A (en) | Tissue-culture rapid propagation method for arundina graminifolia | |
| CN102668985B (en) | Rapid reproduction method for angelica keiskei koidzumi | |
| CN102860257B (en) | Houttuynia cordata aerial stem tissue culture rapid propagation method | |
| CN100394845C (en) | In-bottle production method of detoxified small seed ball of east lily | |
| CN103947548A (en) | Method for establishing agapanthus high-frequency regeneration system | |
| CN114027182A (en) | Tissue culture propagation method for dolichos succulent plants in crassulaceae echeveria | |
| CN103651141A (en) | Factory-like rapid propagation method for test-tube seedlings of dendranthema morifolium | |
| CN102792889B (en) | Chuanminshen violaceum tissue culture rapid propagation technology | |
| CN102138527B (en) | Method for culturing tissue culture seedlings of glabrous greenbrier rhizome | |
| CN103609444A (en) | Tissue culture method for hemerocallis sempervirens araki | |
| CN104026021B (en) | Tulip tissue culture and rapid propagation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DD01 | Delivery of document by public notice |
Addressee: China Three Gorges Corporation patent contact Document name: Notification to Make Rectification |
|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |