CN112056213A - Corn haploid bud seedling chemical doubling complete equipment and doubling method - Google Patents
Corn haploid bud seedling chemical doubling complete equipment and doubling method Download PDFInfo
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- CN112056213A CN112056213A CN202011032681.1A CN202011032681A CN112056213A CN 112056213 A CN112056213 A CN 112056213A CN 202011032681 A CN202011032681 A CN 202011032681A CN 112056213 A CN112056213 A CN 112056213A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
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Abstract
The invention relates to a corn haploid sprout chemical doubling complete equipment, which comprises an automatic corn haploid seed germination device, an automatic corn haploid sprout doubling treatment device and an automatic corn haploid sprout cleaning device, and compared with the prior art, the invention has the advantages and positive effects that: the corn haploid seed germination, colchicine liquid medicine double soaking treatment and bud seedling cleaning links are all automated, the corn haploid seed germination link is improved to improve the germination amount and the germination rate, the bud seedling damage rate is reduced, the colchicine liquid medicine soaking treatment link is improved to increase the amount of the bud seedlings treated by unit colchicine liquid medicine, the risk of contact poisoning of a medicament is avoided, the improvement of the bud seedling cleaning link can also avoid the risk of contact poisoning of the medicament, and a PLC (programmable logic controller) used in the invention can realize remote operation and monitoring of equipment.
Description
Technical Field
The invention relates to the technical field of agricultural breeding, in particular to complete equipment and a doubling method for chemically doubling corn haploid sprouts.
Background
The basic process of the existing corn haploid sprout method chemical doubling technology is as follows: 1. germinating in artificial light incubator or germinating room; 2. manually cutting off the coleoptile tip when the bud grows to 2-3 cm; 3. the cut corn haploid buds are soaked in colchicine solution for 10 hours, then washed, and finally planted into a plug tray or directly planted into a field. There are major problems: the whole process needs manual operation, the operation flow is not easy to control, and the failure is easy. The risk of poisoning is present during the soaking and bud seedling cleaning with colchicine, and the colchicine reagent is expensive, and the solution after soaking can not be reused.
The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.
Disclosure of Invention
The invention aims to solve the problems and provide a complete set of corn haploid bud chemical doubling equipment and a doubling method.
In order to achieve the purpose, the invention adopts the technical scheme that: a corn haploid bud seedling chemical doubling complete equipment comprises a corn haploid seed automatic germination device, a corn haploid bud seedling automatic doubling treatment device and a corn haploid bud seedling automatic cleaning device;
the automatic germination device for the corn haploid seeds comprises a germination cabin, a water leakage port I, two frames, a plurality of germination accelerating boxes, a plurality of germination boxes, two spraying pipes, a heating pipe I, a heating pipe II, a PLC (programmable logic controller) and a water tank I, wherein the germination accelerating cabin and the germination cabin are separated by a partition plate, the two frames are vertically installed and fixed in the germination accelerating cabin and the germination cabin respectively, the germination accelerating boxes are placed on the frames in the germination accelerating cabin, the germination boxes are placed on the frames in the germination cabin, the two spraying pipes are long and short and are arranged right above the frames side by side, the longer spraying pipe extends into the germination cabin and is used for spraying water to the germination boxes below, the shorter spraying pipe is arranged in the germination cabin and is used for spraying water to the germination boxes below, the heating pipe I is arranged in the germination accelerating cabin, and the heating pipe II is arranged in the germination cabin;
the automatic doubling treatment device for the corn haploid sprouts comprises a doubling treatment cabin, liquid medicine tanks, a spraying device, a supporting frame, a doubling treatment box, a liquid medicine circulating pipeline, a PLC (programmable logic controller) II and a liquid medicine circulating pump, wherein the spraying device is used for spraying colchicine liquid medicine, the supporting frame is arranged below the spraying device and is fixed in the doubling treatment cabin through screws, the doubling treatment boxes are placed on the supporting frame and are arranged in multiple layers, the liquid medicine tanks are arranged below the supporting frame, and the liquid medicine tanks are used for containing the colchicine liquid medicine; the liquid medicine circulating pump is connected with the PLC, the liquid medicine circulating pipeline comprises a liquid guide pipe and a liquid medicine box liquid outlet pipe, the liquid medicine box liquid outlet pipe is connected between the liquid medicine box and the liquid medicine circulating pump, and the liquid guide pipe is connected between the spraying device and the liquid medicine circulating pump;
corn haploid bud seedling self-cleaning device is including wasing cabin, hourglass mouth two, support frame, a plurality of washing case, spray thrower, PLC controller three and water tank two, and hourglass mouth two sets up in wasing the cabin bottom, and the support frame is installed and is fixed in wasing the under-deck vertically, has placed on the support frame and has washd the case, it is used for holding the bud after colchicine liquid medicine soaks to wash the case, and the spray thrower sets up directly over the support frame.
Further, the bottom of the automatic corn haploid seed germination device is provided with a moving wheel.
Furthermore, a light-transmitting plate is arranged on the top of the germination accelerating cabin and the top of the germination accelerating cabin, and a plurality of air holes are formed in the light-transmitting plate.
Furthermore, a temperature sensor and a humidity sensor which are respectively in communication connection with the PLC are arranged in the germination accelerating cabin and the germination cabin.
Furthermore, a heat exchange fan is arranged in the germination accelerating cabin and connected with the PLC.
Further, the first water tank is arranged in the germination accelerating cabin and located below the frame, a first limiting ball, a first water pump and a first heating pipe are arranged in the first water tank, one end of a water inlet pipe extends into the first water tank, the other end of the water inlet pipe is connected with an external water source, the bottom of the first water tank is conical and is provided with a water drainage port pipe, a water drainage valve is installed on the water drainage port pipe, the opening and the closing of the water drainage valve are controlled by a PLC (programmable logic controller), the water pump is connected with a spray pipe through a first water outlet pipe, the water pump is connected with the first PLC, and the limiting ball is arranged.
Further, set up the scavenging pump in the water tank two, in a water intake pipe one end stretched into the water tank two, the external water source was connected to the other end, two bottoms of water tank were the toper and were equipped with the outlet, installation drain valve on the outlet, the scavenging pump passes through outlet pipe two and is connected with the spray thrower, the scavenging pump links to each other with PLC controller three-phase.
The method for chemically doubling the corn haploid by the complete set of chemical doubling equipment for the corn haploid sprouts comprises the following steps:
1) cleaning corn haploid seeds, uniformly placing the corn haploid seeds in a germination accelerating box, culturing for 2 days, heating by a heating pipe to keep the temperature in the germination accelerating cabin at 25 ℃, and spraying and supplementing water by a spraying pipe in time; transferring the germinated haploid seeds from the germination accelerating box to a germination box for continuous germination for 2-3 days after 2 days, heating by a second heating pipe to keep the temperature in the germination cabin at 25 ℃, and timely spraying and supplementing water through a spray pipe;
2) when the corn haploid sprouts grow to 3-5cm, the corn haploid sprouts are taken out of the germination box gently, the coleoptile tips of the corn haploid sprouts are cut off manually, and true leaves and growing points are not damaged, so that the corn haploid sprouts with the coleoptile tips cut off are obtained;
3) placing the corn haploid sprouts with the top ends of the coleoptiles cut off in a doubling treatment box, pumping colchicine liquid medicine into the doubling treatment box through a liquid medicine circulating pump, spraying the liquid medicine out through a spray head of a spraying device, and spraying the doubling treatment box;
4) taking the buds subjected to doubling treatment out of the doubling treatment box, putting the buds into a cleaning cabin in a cleaning box, and spraying clear water through a sprayer to clean liquid medicine on the buds;
5) and transplanting the cleaned plumule into a plug tray in time, placing the plug tray in a greenhouse for culturing until 4-5 leaves are planted in the field, and directly transplanting the cleaned plumule into the field.
Compared with the prior art, the invention has the advantages and positive effects that: the corn haploid seed germination, colchicine liquid medicine double soaking treatment and bud seedling cleaning links are all automated, the corn haploid seed germination link is improved to improve the germination amount and the germination rate, the bud seedling damage rate is reduced, the colchicine liquid medicine soaking treatment link is improved to increase the amount of the bud seedlings treated by unit colchicine liquid medicine, the risk of contact poisoning of a medicament is avoided, the improvement of the bud seedling cleaning link can also avoid the risk of contact poisoning of the medicament, and a PLC (programmable logic controller) used in the invention can realize remote operation and monitoring of equipment.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic structural diagram of a corn haploid sprout chemical doubling plant according to a preferred embodiment of the present invention;
fig. 2 is a schematic structural diagram of an automatic corn haploid seed germination device in fig. 1;
FIG. 3 is a schematic structural diagram of an automatic corn haploid sprout doubling device in FIG. 1;
FIG. 4 is a schematic structural diagram of an automatic cleaning device for corn haploid sprouts in FIG. 1;
in the figure: an automatic corn haploid seed germination device 1; a germination accelerating cabin 111; a germination chamber 112; a first water leakage port 12; a frame 13; a support portion 131; a germination accelerating box 141; a germination box 142; a shower pipe 15; a water jet 151; a second heating pipe 162; a fixing member 163; a PLC controller I17; a first water tank 18; a limit ball 181; a water pump 182; heating a first 183; a water inlet pipe 184; an electric valve 185; a first water outlet pipe 186; a drain port tube 187; a drain valve 188; a drain pipe 189; a temperature sensor 191; a humidity sensor 192; a partition 101; a moving wheel 116; a light-transmitting plate 113; air holes 114; a heat exchange fan 115;
a corn haploid sprout automatic doubling processing device 2; a doubling treatment cabin 21; a liquid medicine tank 22; a liquid outlet 221; a spray device 23; a spray head 231; a support frame 24; a doubling treatment tank 25; a liquid guide tube 261; a liquid outlet pipe 262 of the liquid medicine box; a second PLC controller 27; a liquid medicine circulation pump 28; a moving wheel 29;
a corn haploid bud seedling automatic cleaning device 3; a cleaning compartment 31; a second water leakage port 32; a support frame 33; a cleaning tank 34; a sprayer 35; water spray holes 351; a third PLC controller 36; a second water tank 37; a purge pump 372; an inlet conduit 374; the flow valve 375; a second water outlet pipe 376; a water discharge port 377; a drain valve 378; water outlet pipe 379; the wheel 38 is moved.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.
As shown in fig. 1 to 4, the corn haploid sprout chemical doubling complete equipment according to a preferred embodiment of the present invention includes an automatic corn haploid seed germination device 1, an automatic corn haploid sprout doubling processing device 2, and an automatic corn haploid sprout cleaning device 3.
The automatic corn haploid seed germination device 1 comprises a germination accelerating cabin 111, a germination cabin 112, a water leakage port I12, two frames 13, a plurality of germination accelerating boxes 141, a plurality of germination accelerating boxes 142, two spray pipes 15, a heating pipe I183, a heating pipe II 162, a PLC (programmable logic controller) I17 and a water tank I18. The germination accelerating cabin 111 and the germination cabin 112 are separated by a partition plate 101. The shell of the automatic corn haploid seed germination device 1 is made of stainless steel, and the size of the shell is as follows: length 1.65 m, width 0.8 m, height 1.7 m. Automatic device 1 that sprouts of maize haploid seed bottom is equipped with removes wheel 116, removes wheel 116 through setting up and is convenient for remove automatic device 1 that sprouts of maize haploid seed.
The seed growth and development need stable temperature and higher humidity, and in order to realize the mechanized production of the seeds, the germination accelerating cabin 111 mainly provides a cultivation environment for the corn haploid seeds, and the germination cabin 112 mainly provides a cultivation environment for the sprouts. A light-transmitting plate 113 is arranged on the top of the germination accelerating cabin 111 and the germination cabin 112, and a plurality of air holes 114 are arranged on the light-transmitting plate 113.
The first PLC 17 is arranged on the outer side wall of the germination cabin 112. PLC controller 17 can carry out intelligent control to automatic device 1 that sprouts of maize haploid seed, improves the intellectuality of machine that sprouts, specifically speaking, PLC controller 17 can be regulated and control the temperature and the humidity in germinating cabin 111 and germinating cabin 112, can also regulate and control the time of drenching water, the interval of drenching water. When the water spraying time or the water spraying interval is set on the control panel by an operator, after the preset time is reached, the PLC 17 can make a corresponding response, and the water in the water tank 18 is sucked to the spray pipes 15 above the germination accelerating tanks 141 through the water pumps 182 to perform water spraying operation.
The two frames 13 are vertically fixed in the germination accelerating compartment 111 and the germination accelerating compartment 112, and a plurality of supporting parts 131 are arranged on each frame 13 at intervals, and the supporting parts 131 are arranged in multiple layers. The frame 13 in the germination accelerating cabin 111 is arranged above the first water tank 18, the germination accelerating boxes 141 are placed on the supporting parts 131 on the frame 13 in the germination accelerating cabin 111, and the germination boxes 142 are placed on the supporting parts 131 on the frame 13 in the germination accelerating cabin 112. The germination accelerating box 141 is used for containing corn haploid seeds, so that the corn haploid seeds can grow and develop in the germination accelerating box 141. The germination box 142 is used for holding sprouts so that the sprouts can grow in the germination box 142.
The two shower pipes 15 are arranged in parallel right above the frame 13. Each spray pipe 15 is provided with a plurality of fine water spray ports 151, and water flows down from the water spray ports 151 of the spray pipes 15, so that the water distribution efficiency is remarkably improved, and the water spraying distribution uniformity is also improved. The spraying pipe 15 sprays water, and the time and frequency of the water spraying are controlled by the PLC I17, so that the automatic water spraying function is realized. The longer spray pipes 15 extend into the germination cabin 112 and are used for spraying water to the corn haploid sprouts in the germination boxes 142 at the lower layers; the shorter spray pipes 15 extend into the germination accelerating cabin 111 and are used for spraying water to the corn haploid seeds in the germination accelerating boxes 141 at the lower layers.
The second heating pipe 162 is arranged in the germination cabin 112 through a fixing part 163 and is located at the bottom of the frame 13, and the second heating pipe 162 is connected with a power supply through an electric wire. The second heating pipe 162 is used for heating the germination cabin 112 to provide the temperature required by the growth of the sprouts, and the heating time and the heating temperature of the second heating pipe 162 are controlled by the first PLC 17.
And a temperature sensor 191 and a humidity sensor 192 which are respectively in communication connection with the first PLC 17 are arranged in the germination accelerating cabin 111 and the germination accelerating cabin 112. When the temperature in the germination accelerating cabin 111 or the germination cabin 112 exceeds or is lower than the optimal temperature required by the growth of seeds, the temperature sensor 191 sends a signal to the first PLC controller 17, and the first PLC controller 17 controls the heating temperature of the first heating pipe 183 or the second heating pipe 162. Similarly, when the humidity inside the germination accelerating compartment 111 or the germination compartment 112 is lower than the optimal humidity required for cultivating seeds, the humidity sensor 192 sends a signal to the first PLC controller 17, and the first PLC controller 17 controls the spray pipe 15 to spray water to the germination accelerating compartment 141 or the germination compartment 142. On the contrary, when the humidity inside the germination accelerating compartment 111 or the germination compartment 112 is higher than the optimal humidity required for cultivating seeds, the humidity sensor 192 sends a signal to the first PLC controller 17, and the first PLC controller 17 controls the spraying pipe 15 to stop the water spraying operation.
The first water leakage port 12 is arranged at the bottom of the germination cabin 112 and is used for being connected with a water drainage pipeline to drain water to a sewer.
A heat transfer fan 115 sets up in vernalization cabin 111, and in heat transfer fan 115's wind direction blew into vernalization cabin 111 and germination cabin 112 for outside-in, heat transfer fan 115 is connected with PLC controller 17, through opening, closing and the amount of wind of PLC controller 17 control heat transfer fan 115.
The first water tank 18 is arranged in the germination accelerating cabin 111 and located below the frame 13, and a limiting ball 181, a stainless steel water pump 182 and a first heating pipe 183 are arranged in the first water tank 18. One end of a water inlet pipe 184 extends into the first water tank 18, and the other end is connected with an external water source. An electric valve 185 is installed on the water inlet pipe 184, and the opening and the closing of the electric valve 185 are controlled by a PLC one 17. The first PLC controller 17 controls the water inlet pipe 184 to supply water to the first water tank 18 or stop supplying water by controlling the opening or closing of the electric valve 185. The bottom of the first water tank 18 is tapered and is provided with a drain pipe 187, a drain valve 188 is mounted on the drain pipe 187, the opening and closing of the drain valve 188 is controlled by the first PLC 17, and the drain pipe 187 is connected with a drain pipe 189 to discharge water to a sewer.
The water pump 182 is connected with the spray pipe 15 through the first water outlet pipe 186, and the water pump 182 is used for pumping the water in the first water tank 18 upwards into the spray pipe 15 so as to enable the water to be sprayed out from the water spray opening 151 on the spray pipe 15. The water pump 182 is connected with a first PLC 17, and the first PLC 17 controls the water spraying of the spraying pipe 15 by periodically controlling the on and off of the water pump 182. The first heating pipe 183 is connected with a power supply through an electric wire, the first heating pipe 183 heats water in the first water tank 18 to provide the temperature required by germination of the corn haploid seeds, and the time and the heating temperature of the first heating pipe 183 for heating the water are controlled by the first PLC 17.
The limiting ball 181 is a hollow ball body and is arranged at the water inlet of the water inlet pipe 184, the limiting ball 181 is lowered and raised under the action of the liquid level in the first water tank 18, after the spraying pipe 15 performs spraying operation, the water level in the first water tank 18 is lowered, then the limiting ball 181 is lowered, at this time, the water inlet pipe 184 is connected with a tap water pipeline, water is added into the first water tank 18, and the limiting ball 181 is also raised continuously along with the rise of the water level in the first water tank 18. Along with the continuous rising of the limiting ball 181, when the water level is increased to the highest water level line, the limiting ball 181 blocks the water inlet of the water inlet pipe 184, and the water inlet is stopped.
The automatic corn haploid sprout seedling doubling device 2 comprises a doubling treatment cabin 21, a liquid medicine tank 22, a spraying device 23, a supporting frame 24, a doubling treatment tank 25, a liquid medicine circulating pipeline, a PLC (programmable logic controller) II 27 and a liquid medicine circulating pump 28.
The shell of the doubling processing cabin 21 is made of stainless steel, and the shell size of the doubling processing cabin 21 is as follows: the length is 0.6 m, the width is 0.8 m, the height is 1.7 m, and a plurality of air holes are arranged on the side wall of the doubling processing cabin 21. The bottom of the doubling treatment cabin 21 is provided with a moving wheel 29, and the automatic doubling treatment device 2 for the corn haploid sprouts is convenient to move by arranging the moving wheel 29. The top of the doubling processing chamber 21 is provided with a spraying device 23, the spraying device 23 comprises a plurality of spray heads 231, and the spraying device 23 is used for spraying colchicine liquid medicine.
A supporting frame 24 is arranged below the spraying device 23, the supporting frame 24 is fixed in the doubling treatment cabin 21 through screws, a doubling treatment box 25 is placed on the supporting frame 24, and the doubling treatment box 25 is arranged in a multilayer mode.
The liquid medicine circulating pump 28 is arranged on the outer side wall of the doubling processing chamber 21, the liquid medicine circulating pump 28 is connected with the second PLC 27, and the second PLC 27 controls the spraying device 23 to spray liquid medicine by periodically controlling the liquid medicine circulating pump 28 to be turned on and off. The liquid medicine circulation pipeline comprises a liquid guide pipe 261 and a liquid medicine box liquid outlet pipe 262, the liquid medicine box liquid outlet pipe 262 is connected between the liquid medicine box 22 and the liquid medicine circulation pump 28, and the liquid guide pipe 261 is connected between the spraying device 23 and the liquid medicine circulation pump 28.
The working principle of the automatic corn haploid sprout doubling device 2 is as follows: the liquid medicine is pumped in by the liquid medicine circulating pump 28, reaches the spray head 231 of the spraying device 23 through the liquid guide pipe 261, is sprayed out by the spray head 231 of the spraying device 23, is sprayed to the doubling treatment box 25, and a part of the liquid medicine falls into the bottom of the liquid medicine box 22 through the doubling treatment box 25 to be collected and circulates to the spraying device 23 through the liquid medicine circulating pipeline, and the liquid medicine is circulated in a reciprocating way.
Corn haploid sprout automatic cleaning device 3 includes cleaning tank 31, two 32 of mouth of a river that leak, support frame 33, a plurality of washing case 34, shower 35, PLC controller three 36 and water tank two 37.
The outer shell of the cleaning cabin 31 is made of stainless steel, and the size of the outer shell of the cleaning cabin 31 is as follows: length 1.0 meter, width 0.8 meter, height 1.7 meter. The top of the cleaning cabin 31 is provided with a plurality of air holes, and the second water leakage port 32 is arranged at the bottom of the cleaning cabin 31 and is used for being connected with a drainage pipeline to discharge water to a sewer. The outer wall of the bottom of the cleaning cabin 31 is provided with a moving wheel 38, and the automatic cleaning device 3 for the corn haploid sprouts is convenient to move by arranging the moving wheel 38.
The support frame 33 is vertically installed and fixed in the cleaning cabin 31, the cleaning box 34 is placed on the support frame 33, the cleaning box 34 is arranged in a multilayer mode, and the cleaning box 34 is used for containing the sprouts soaked by colchicine liquid medicine.
The sprayer 35 is arranged right above the support frame 33, the plurality of small water spraying holes 351 are formed in the sprayer 35, water flows down from the water spraying holes 351 of the sprayer 35, the water distribution efficiency is obviously improved, and the uniformity of water spraying distribution is also improved. The sprayer 35 sprays water, and the time and frequency of the water spraying are controlled by the PLC III 36, so that the automatic water spraying function is realized.
A stainless steel cleaning pump 372 is arranged in the second water tank 37, one end of a water inlet pipeline 374 extends into the second water tank 37, and the other end of the water inlet pipeline is connected with an external water source. The flow valve 375 is installed on the water inlet pipeline 374, and the opening and the closing of the flow valve 375 are controlled by the PLC III 36. The third PLC 36 controls the water inlet 374 to supply water to the second water tank 37 or stop supplying water by controlling the flow valve 375 to be opened or closed. The bottom of the second water tank 37 is conical and is provided with a water outlet 377, a drain valve 378 is installed on the water outlet 377, the opening and the closing of the drain valve 378 are controlled by a third PLC controller 36, and the water outlet 377 is connected with a water outlet pipe 379 to discharge water to a sewer.
The cleaning pump 372 is connected with the sprayer 35 through a second water outlet pipe 376, and the cleaning pump 372 is used for pumping the water in the second water tank 37 upwards into the sprayer 35 so as to enable the water to be sprayed out from the water spraying holes 351 in the sprayer 35. The cleaning pump 372 is connected with the third PLC 36, and the third PLC 36 controls the sprayer 35 to spray water by periodically controlling the cleaning pump 372 to be turned on and off.
The third PLC 36 is arranged on the outer side wall of the cleaning cabin 31, and the third PLC 36 can regulate and control cleaning time and cleaning time interval. When the cleaning time or the cleaning time interval is set on the control panel by an operator, after the preset time is reached, the PLC three 36 can make a corresponding response, and the water in the water tank two 37 is sucked to the sprayer 35 above each layer of cleaning tank 34 through the cleaning pump 372 to perform the cleaning operation.
The method for chemically doubling the corn haploid by using the device comprises the following steps:
1) cleaning corn haploid seeds, uniformly placing the corn haploid seeds in a germination accelerating box 141, culturing for 2 days, heating by a first heating pipe 183 to keep the temperature in the germination accelerating cabin 111 at 25 ℃, and spraying and supplementing water by a spraying pipe 15 in time; transferring the germinated haploid seeds from the germination accelerating box 141 to the germination box 142 for continuous germination for 2-3 days after 2 days, heating the second heating pipe 162 to keep the temperature in the germination cabin 112 at 25 ℃, and spraying and supplementing water through the spraying pipe 15 in time;
2) when the corn haploid sprouts grow to 3-5cm, the corn haploid sprouts are taken out of the germination box 142 gently, the coleoptile tips of the corn haploid sprouts are cut off manually, and true leaves and growing points are not damaged, so that the corn haploid sprouts with the coleoptile tips cut off are obtained;
3) placing the corn haploid sprouts with the top ends of the coleoptiles cut off in a doubling treatment box 25, pumping colchicine liquid medicine into the doubling treatment box through a liquid medicine circulating pump 28, spraying the liquid medicine out through a spray head 231 of a spraying device 23, and spraying the doubling treatment box 25;
4) taking the buds subjected to doubling treatment out of the doubling treatment box 25, putting the buds into a cleaning cabin 31 in a cleaning box 34, and spraying clear water through a sprayer 35 to clean liquid medicine on the buds;
5) and transplanting the cleaned plumule into a plug tray in time, placing the plug tray in a greenhouse for culturing until 4-5 leaves are planted in the field, and directly transplanting the cleaned plumule into the field.
The above descriptions of the embodiments of the present invention, which are not related to the above description, are well known in the art, and may be implemented by referring to the well-known technologies.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (8)
1. The utility model provides a maize haploid bud seedling chemical doubling complete sets which characterized in that: the automatic corn haploid seed germinating device comprises a corn haploid seed automatic germinating device, a corn haploid bud seedling automatic doubling treatment device and a corn haploid bud seedling automatic cleaning device;
the automatic germination device for the corn haploid seeds comprises a germination cabin, a water leakage port I, two frames, a plurality of germination accelerating boxes, a plurality of germination boxes, two spraying pipes, a heating pipe I, a heating pipe II, a PLC (programmable logic controller) and a water tank I, wherein the germination accelerating cabin and the germination cabin are separated by a partition plate, the two frames are vertically installed and fixed in the germination accelerating cabin and the germination cabin respectively, the germination accelerating boxes are placed on the frames in the germination accelerating cabin, the germination boxes are placed on the frames in the germination cabin, the two spraying pipes are long and short and are arranged right above the frames side by side, the longer spraying pipe extends into the germination cabin and is used for spraying water to the germination boxes below, the shorter spraying pipe is arranged in the germination cabin and is used for spraying water to the germination boxes below, the heating pipe I is arranged in the germination accelerating cabin, and the heating pipe II is arranged in the germination cabin;
the automatic doubling treatment device for the corn haploid sprouts comprises a doubling treatment cabin, liquid medicine tanks, a spraying device, a supporting frame, a doubling treatment box, a liquid medicine circulating pipeline, a PLC (programmable logic controller) II and a liquid medicine circulating pump, wherein the spraying device is used for spraying colchicine liquid medicine, the supporting frame is arranged below the spraying device and is fixed in the doubling treatment cabin through screws, the doubling treatment boxes are placed on the supporting frame and are arranged in multiple layers, the liquid medicine tanks are arranged below the supporting frame, and the liquid medicine tanks are used for containing the colchicine liquid medicine; the liquid medicine circulating pump is connected with the PLC, the liquid medicine circulating pipeline comprises a liquid guide pipe and a liquid medicine box liquid outlet pipe, the liquid medicine box liquid outlet pipe is connected between the liquid medicine box and the liquid medicine circulating pump, and the liquid guide pipe is connected between the spraying device and the liquid medicine circulating pump;
corn haploid bud seedling self-cleaning device is including wasing cabin, hourglass mouth two, support frame, a plurality of washing case, spray thrower, PLC controller three and water tank two, and hourglass mouth two sets up in wasing the cabin bottom, and the support frame is installed and is fixed in wasing the under-deck vertically, has placed on the support frame and has washd the case, it is used for holding the bud after colchicine liquid medicine soaks to wash the case, and the spray thrower sets up directly over the support frame.
2. The corn haploid sprout chemical doubling kit of claim 1, wherein: the bottom of the automatic corn haploid seed germination device is provided with a movable wheel.
3. The corn haploid sprout chemical doubling kit of claim 1, wherein: a light-transmitting plate is arranged on the germination accelerating cabin and the top of the germination accelerating cabin, and a plurality of air holes are arranged on the light-transmitting plate.
4. The corn haploid sprout chemical doubling kit of claim 1, wherein: and the germination accelerating cabin and the germination cabin are internally provided with a temperature sensor and a humidity sensor which are respectively in communication connection with the PLC controller.
5. The corn haploid sprout chemical doubling kit of claim 1, wherein: a heat exchange fan is arranged in the germination accelerating cabin and connected with the PLC.
6. The corn haploid sprout chemical doubling kit of claim 1, wherein: the first water tank is arranged in the germination accelerating cabin and located below the frame, a first limiting ball, a first water pump and a first heating pipe are arranged in the first water tank, one end of a water inlet pipe stretches into the first water tank, the other end of the water tank is connected with an external water source, the bottom of the first water tank is conical and is provided with a water drainage port pipe, a water drainage valve is installed on the water drainage port pipe, the opening and the closing of the water drainage valve are controlled by a PLC (programmable logic controller), the water pump is connected with a spray pipe through a first water outlet pipe, the water pump is connected with the first PLC, and the limiting ball is.
7. The corn haploid sprout chemical doubling kit of claim 1, wherein: the cleaning pump is arranged in the second water tank, one end of a water inlet pipeline extends into the second water tank, the other end of the water inlet pipeline is connected with an external water source, the bottom of the second water tank is conical and is provided with a water outlet, a drain valve is installed on the water outlet, the cleaning pump is connected with the sprayer through a second water outlet pipe, and the cleaning pump is connected with the three phases of the PLC.
8. A method for chemically doubling corn haploids by means of the corn haploid sprout chemical doubling plant of any one of claims 1 to 7, characterized in that: the method comprises the following steps:
1) cleaning corn haploid seeds, uniformly placing the corn haploid seeds in a germination accelerating box, culturing for 2 days, heating by a heating pipe to keep the temperature in the germination accelerating cabin at 25 ℃, and spraying and supplementing water by a spraying pipe in time; transferring the germinated haploid seeds from the germination accelerating box to a germination box for continuous germination for 2-3 days after 2 days, heating by a second heating pipe to keep the temperature in the germination cabin at 25 ℃, and timely spraying and supplementing water through a spray pipe;
2) when the corn haploid sprouts grow to 3-5cm, the corn haploid sprouts are taken out of the germination box gently, the coleoptile tips of the corn haploid sprouts are cut off manually, and true leaves and growing points are not damaged, so that the corn haploid sprouts with the coleoptile tips cut off are obtained;
3) placing the corn haploid sprouts with the top ends of the coleoptiles cut off in a doubling treatment box, pumping colchicine liquid medicine into the doubling treatment box through a liquid medicine circulating pump, spraying the liquid medicine out through a spray head of a spraying device, and spraying the doubling treatment box;
4) taking the buds subjected to doubling treatment out of the doubling treatment box, putting the buds into a cleaning cabin in a cleaning box, and spraying clear water through a sprayer to clean liquid medicine on the buds;
5) and transplanting the cleaned plumule into a plug tray in time, placing the plug tray in a greenhouse for culturing until 4-5 leaves are planted in the field, and directly transplanting the cleaned plumule into the field.
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