CN110115228A - A kind of monoploid young shoot doubles the device and method of processing - Google Patents
A kind of monoploid young shoot doubles the device and method of processing Download PDFInfo
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- CN110115228A CN110115228A CN201910206200.5A CN201910206200A CN110115228A CN 110115228 A CN110115228 A CN 110115228A CN 201910206200 A CN201910206200 A CN 201910206200A CN 110115228 A CN110115228 A CN 110115228A
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- monoploid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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Abstract
The invention belongs to agricultural technology fields, the device of processing is doubled more particularly to a kind of monoploid young shoot, the shell of first spray head is provided with including medicine container and top, first spray head is connected to by drug liquid tube with medicine container, it is provided with the first booster pump on the pipeline of drug liquid tube, layering frame is provided in shell, the bottom of shell is provided with hydrops bucket, hydrops bucket is connected with return pipe, and return pipe is connected to medicine container；Monoploid young shoot of the invention doubles the apparatus structure advantages of simple of processing, when carrying out chemicals treatment to sprout, medical fluid flows through drug liquid tube and is sprayed in sprout from first spray head again, hydrops bucket is fallen under medical fluid, medicine container is back to through return pipe again, formation circulates processing mode, the method for doubling processing using the monoploid young shoot of the device is easy to operate, avoid the problems such as static direct phytotoxicity for impregnating sprout generation of medical fluid is serious, survival rate is low, and medical fluid can be saved, it reduces environmental pollution, agricultural technology field can be widely used in.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of monoploid young shoot doubles the device and method of processing.
Background technique
Homozygous inbred lines can be quickly obtained using haploid breeding, i.e. DH (Doubled haploid, DH) system accelerates
Breeding process has become the main method of modern breeding.It obtains enough monoploid and doubles to obtain the base that DH system is DH breeding
This link.Monoploid Natural doubling rate is generally lower, takes artificial treatment to improve and doubles rate to meet breeding and correlative study
It needs.Artificial treatment is more various using reagent and the method doubled, including the chemical reagent such as colchicine, herbicide, uses
The methods of method for soaking seed, leaching root method, leaching sprout method, injection method, drop heart method, wherein leaching sprout method is using relatively broad.Existing processing
Method usually uses medical fluid to impregnate entire sprout, and not only bud top absorbs medicament, and sprout root also absorbs medicament, cause sprout by
Phytotoxicity is serious, and sprout survival rate is low, or deformity, male and female are uncoordinated etc., and need dose very big, and environmental pollution is serious.
Summary of the invention
In order to solve the above problems existing in the present technology, the present invention provides the dresses that a kind of monoploid young shoot doubles processing
Set, for the method for doubling of the prior art generate plant phytotoxicity is serious, survival rate is low and lopsided and situations such as need dose big
And research and develop, simple and reasonable, effectively increase sprout doubles rate and survival rate, and reduces medical fluid usage amount, reduces
Environmental pollution.
The present invention implements by the following technical programs:
A kind of monoploid young shoot doubles the device of processing, including shell and medicine container, is provided at the top of the shell
First spray head, the first spray head are connected to by drug liquid tube with the medicine container, and the is provided on the pipeline of the drug liquid tube
One booster pump.
The layering frame for placing sprout is provided in the shell, the bottom of the shell is provided with the hydrops of taper
Bucket, the hydrops bucket are connected with return pipe, and the return pipe is connected to the medicine container.
Further, the device that monoploid young shoot of the invention doubles processing further includes fresh water container, the top of the shell
Portion is provided with second spray head, and the second spray head is connected to the fresh water container by water pipe, is arranged on the pipeline of the water pipe
There is the second booster pump.
Further, it is also connected with below the hydrops bucket and is provided with drain pipe, be provided on the pipeline of the return pipe
For controlling the first valve of the return pipe on-off, second for controlling the drain pipe on-off is provided on the drain pipe
Valve.
Further, the first spray head and the second spray head are atomizer.
Further, the layering frame includes multiple object placement nets being distributed along the vertical direction, the neighbouring glove
Net is staggered.
Further, the action pane for being put into or taking out sprout, the operation are provided on the side wall of the shell
It is provided with cabinet door at window, is additionally provided with sealing strip between the cabinet door and the shell.
Further, the device that monoploid young shoot of the invention doubles processing further includes rack, and the shell, medicament hold
Device, fresh water container, the first booster pump and the second booster pump are arranged in the rack, and the bottom of the rack is provided with rolling
Wheel.
Further, the hydrops bucket is located at the top of the medicine container and the fresh water container.
The present invention also provides a kind of method that monoploid young shoot doubles processing, this method includes using above-mentioned monoploid
Young shoot doubles the device of processing, specifically includes the following steps:
S1: the impurity in cleaning sprout；
S2: the sprout after pretreatment cleaning is put on the layering frame；
S3: the first pressurization pump work, makes the medicine liquid spray in medicine container carry out medical fluid processing in sprout；
S4: treated that sprout passes through water cleaning treatment again for medical fluid.
Further, the step S3 Chinese medicine liquid processing time is 8h~12h.
Further, the water cleaning treatment time is 0.5h~1h in the step S4.
Based on above technical scheme, technical effect of the invention are as follows:
Monoploid young shoot of the invention doubles the apparatus structure advantages of simple of processing, when carrying out chemicals treatment to sprout,
Under the pressurization of the first booster pump, the medical fluid in medicine container flows through drug liquid tube and is sprayed in sprout from first spray head again,
Hydrops bucket is fallen under medical fluid, then is back to medicine container through return pipe, realizes the recycling of medical fluid；And use the list of the device
The method that times body young shoot doubles processing is easy to operate, both avoided medical fluid directly impregnates the phytotoxicity of sprout generation seriously, survival rate
The problems such as low, and medical fluid can be made to be recycled, medical fluid is saved, environmental pollution is reduced.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the structural schematic diagram for the device that the monoploid young shoot of the embodiment of the present invention 1 doubles processing；
Fig. 2 is the structural schematic diagram for the device that the monoploid young shoot of the embodiment of the present invention 2 doubles processing；
Fig. 3 is the structural schematic diagram for the device that the monoploid young shoot of the embodiment of the present invention 3 doubles processing；
In figure:
1- shell, 101- cabinet door；2- medicine container；3- first spray head；4- drug liquid tube；The first booster pump of 5-；6- is layered frame,
601- object placement net；7- hydrops bucket；8- return pipe；9- fresh water container；10- second spray head；11- water pipe；The second booster pump of 12-；13-
Drain pipe；The first valve of 14-；The second valve of 15-；16- rack；17- idler wheel.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
Embodiment 1:
As shown in Figure 1, present embodiments providing the device that a kind of monoploid young shoot doubles processing, the monoploid young shoot adds
The device of processing includes shell 1 and medicine container 2 again, and the top of the shell 1 is provided with first spray head 3, the first spray head 3
It is connected to by drug liquid tube 4 with the medicine container 2, the first booster pump 5 is provided on the pipeline of the drug liquid tube 4.
The layering frame 6 for placing sprout is provided in the shell 1, the bottom of the shell 1 is provided with the product of taper
Liquid bucket 7, the hydrops bucket 7 are connected with return pipe 8, and the return pipe 8 is connected to the medicine container 2.
The monoploid young shoot of the present embodiment doubles the device of processing when specifically used, and processing is provided in medicine container 2
Medicament, such as colchicine or herbicide chemical reagent, sprout is put on the layering frame 6 in shell 1, needs to carry out sprout
When doubling processing, the first booster pump 5 is opened, so that the medicament in medicine container 2 is flowed through drug liquid tube 4 and is sprayed again through first spray head 3, made
Medicament is attached in the sprout in shell 1.
The monoploid young shoot of the present embodiment doubles the device of processing in specific setting, and hydrops bucket 7 holds higher than the medicament
Device 2 enables in the medicament natural back flow to medicine container 2 in hydrops bucket 7.
As further improvement of this embodiment, the first spray head 3 be atomizer, it is ensured that sprinkling it is uniform.
As further improvement of this embodiment, the layering frame 6 includes multiple object placement nets being distributed along the vertical direction
601, the neighbouring object placement net 601 is staggered, and about 601 object placement net is staggered conducive in the sprout attachment of lower layer
Medicament, preventing upper layer sprout from blocking makes only a small number of lower layer's sprouts adhere to upper medicament.
As further improvement of this embodiment, it is provided on the side wall of the shell 1 for being put into or taking out sprout
Action pane is provided with cabinet door 101 at the action pane, when needing to be put into or takes out sprout, opens cabinet door 101 and operates and is
Can, and when needing to carry out sprout processing sprinkling medicament, then need to close cabinet door 101, as preferably selecting, the cabinet door with
Sealing strip is additionally provided between the shell.
As further improvement of this embodiment, the internal diameter of the return pipe 8 is greater than the internal diameter of the drug liquid tube 4, avoids
Medicament excessively contains in hydrops bucket 7, guarantee medicine container 2 there is medicament to be sprayed always, realize medicament sprinkling and recycling
Circulation.
Embodiment 2:
The present embodiment is that further improvement on the basis of above-described embodiment 1 is especially adopted for the present invention is better achieved
With following structures:
The device that the monoploid young shoot of the present embodiment doubles processing further includes fresh water container 9, the top setting of the shell 1
There is second spray head 10, the second spray head 10 is connected to the fresh water container 9 by water pipe 11, is set on the pipeline of the water pipe 11
It is equipped with the second booster pump 12.
The lower section of the hydrops bucket 7, which is also connected with, is provided with drain pipe 13, is provided on the pipeline of the return pipe 8 for controlling
The first valve 14 of 8 on-off of return pipe is made, for controlling 13 on-off of drain pipe is provided on the drain pipe 13
Two valves 15.
The monoploid young shoot of the present embodiment doubles the device of processing when specifically used, point sprout being put into shell 1
In layer frame 6, when needing to handle sprout, the first booster pump 5 is opened, closes the second booster pump 12, opens the first valve 14, is closed
The second valve 15 is closed, so that the medicament in medicine container 2 is flowed through drug liquid tube 4 and is sprayed again through first spray head 3, medicament is made to be attached to shell 1
In interior sprout, monoploid sprout forms amphiploid；
After chemicals treatment, the first booster pump 5 is closed, opens the second booster pump 12, closes the first valve 14, opens the
Two valves 15 make the aqueous cleaning in fresh water container 9 flow through water pipe 11 and spray again through second spray head 10, to medicament remaining in sprout
It is cleaned, the waste liquid for handling generation is discharged through drain pipe 13.
As further improvement of this embodiment, the first spray head 3 and the second spray head 10 are atomizer.
Embodiment 3:
The present embodiment is that further improvement on the basis of above-described embodiment 2 is especially adopted for the present invention is better achieved
With following structures:
The device that the monoploid young shoot of the present embodiment doubles processing further includes rack 16, the shell 1, medicine container 2, clear
Water container 9, the first booster pump 5 and the second booster pump 12 are arranged in the rack 16, and the bottom of the rack 16 is provided with
Idler wheel 17, moves integrally whole device.
As further improvement of this embodiment, the hydrops bucket 7 is located at the medicine container 2 and the fresh water container 9
Top, can make in the medical fluid natural back flow to medicine container 2 of hydrops bucket 7 in this way.
Embodiment 4:
A kind of method that monoploid young shoot doubles processing is present embodiments provided, this method uses above-described embodiment 1 or 2 institutes
The monoploid young shoot stated doubles the device of processing, specifically includes the following steps:
S1: the impurity in cleaning sprout；
S2: the sprout after pretreatment cleaning is put on the layering frame 6；
The work of S3: the first booster pump 5, makes the medicine liquid spray in medicine container 2 carry out medical fluid processing in sprout；
S4: treated that sprout passes through water cleaning treatment again for medical fluid.
Specifically, it in above-mentioned steps S1, is pre-processed using clear water cleaning sprout, removes the impurity in sprout；
In above-mentioned steps S4:
It, can be by the sprout after drug-treated from shell 1 if doubling the device of processing using the monoploid young shoot of embodiment 1
Remaining medicament in sprout is cleaned with clear water again after interior taking-up；
If doubling the device of processing using the monoploid young shoot of embodiment 2, water can be passed through by the second booster pump 12
Sprout is cleaned in the sprinkling of second spray head 10.
As further improvement of this embodiment, the step S3 Chinese medicine liquid processing time is 8h~12h.
As further improvement of this embodiment, the water cleaning treatment time is 0.5h~1h in the step S4.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.
Claims (10)
1. the device that a kind of monoploid young shoot doubles processing, it is characterised in that: including shell (1) and medicine container (2), the shell
It is provided at the top of body (1) first spray head (3), the first spray head (3) is connected by drug liquid tube (4) and the medicine container (2)
It is logical, the first booster pump (5) are provided on the pipeline of the drug liquid tube (4)；
The layering frame (6) for placing sprout is provided in the shell (1), the bottom of the shell (1) is provided with taper
Hydrops bucket (7), the hydrops bucket (7) are connected with return pipe (8), and the return pipe (8) and the medicine container (2) are even
It is logical.
2. the device that monoploid young shoot according to claim 1 doubles processing, it is characterised in that: further include fresh water container
(9), it is provided with second spray head (10) at the top of the shell (1), the second spray head (10) passes through water pipe (11) and described clear
Water container (9) is connected to, and is provided with the second booster pump (12) on the pipeline of the water pipe (11)；
It is also connected with and is provided with drain pipe (13) below the hydrops bucket (7), be provided with and be used on the pipeline of the return pipe (8)
First valve (14) of the return pipe (8) on-off is controlled, is provided on the drain pipe (13) for controlling the drain pipe
(13) the second valve (15) of on-off.
3. the device that monoploid young shoot according to claim 2 doubles processing, it is characterised in that: the first spray head (3)
It is atomizer with the second spray head (10).
4. the device that monoploid young shoot according to claim 1 doubles processing, it is characterised in that: layering frame (6) packet
Multiple object placement nets (601) being distributed along the vertical direction are included, the neighbouring object placement net (601) is staggered.
5. the device that monoploid young shoot according to claim 1 doubles processing, it is characterised in that: the side of the shell (1)
It is provided with the action pane for being put into or taking out sprout on wall, cabinet door (101), the cabinet door are provided at the action pane
(101) sealing strip is additionally provided between the shell (1).
6. doubling the device of processing according to any monoploid young shoot of claim 2~5, it is characterised in that: further include machine
Frame (16), the shell (1), medicine container (2), fresh water container (9), the first booster pump (5) and the second booster pump (12) are all provided with
It sets on the rack (16), the bottom of the rack (16) is provided with idler wheel (17).
7. the device that monoploid young shoot according to claim 6 doubles processing, it is characterised in that: hydrops bucket (7) position
In the top of the medicine container (2) and the fresh water container (9).
8. a kind of method that monoploid young shoot doubles processing, it is characterised in that: this method is any described using claim 1~7
Monoploid young shoot double processing device, comprising the following steps:
S1: the impurity in cleaning sprout；
S2: the sprout after pretreatment cleaning is put on the layering frame (6)；
S3: the first booster pump (5) work, makes the medicine liquid spray in medicine container (2) carry out medical fluid processing in sprout；
S4: treated that sprout passes through water cleaning treatment again for medical fluid.
9. the method that monoploid young shoot according to claim 8 doubles processing, it is characterised in that: the step S3 Chinese medicine liquid
The processing time is 8h~12h.
10. the method that monoploid young shoot according to claim 8 doubles processing, it is characterised in that: water in the step S4
The cleaning treatment time is 0.5h~1h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910206200.5A CN110115228A (en) | 2019-03-18 | 2019-03-18 | A kind of monoploid young shoot doubles the device and method of processing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910206200.5A CN110115228A (en) | 2019-03-18 | 2019-03-18 | A kind of monoploid young shoot doubles the device and method of processing |
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| Publication Number | Publication Date |
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| CN110115228A true CN110115228A (en) | 2019-08-13 |
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| CN201910206200.5A Pending CN110115228A (en) | 2019-03-18 | 2019-03-18 | A kind of monoploid young shoot doubles the device and method of processing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112056213A (en) * | 2020-09-27 | 2020-12-11 | 山西省农业科学院玉米研究所 | Corn haploid bud seedling chemical doubling complete equipment and doubling method |
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