CN204888280U - Plant tissue culture explant degassing unit - Google Patents
Plant tissue culture explant degassing unit Download PDFInfo
- Publication number
- CN204888280U CN204888280U CN201520608485.2U CN201520608485U CN204888280U CN 204888280 U CN204888280 U CN 204888280U CN 201520608485 U CN201520608485 U CN 201520608485U CN 204888280 U CN204888280 U CN 204888280U
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- CN
- China
- Prior art keywords
- disinfection
- sterilizing
- bottle
- explant
- filter screen
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Abstract
The utility model discloses a plant tissue culture explant degassing unit, including disinfection bottle, a disinfection section of thick bamboo, filter screen and upper cover, in disinfection bottle and a disinfection section of thick bamboo on the middle part respectively fixed mounting have a filter screen, the lower extreme and the disinfection bottle open end thread sealing of a disinfection section of thick bamboo are connected and are linked together with the disinfection bottle, the upper cover lock is in the upper end of disinfection tin, is close to bottom surface department in the lower part of disinfecting bottle and is equipped with the leakage fluid dram, installs a tap on the leakage fluid dram. The utility model discloses simple structure, convenient operation. Through setting up a disinfection bottle and a disinfection section of thick bamboo, can disinfect to the explant of difference simultaneously, work load in the time of reducing the experiment improves work efficiency, during the disinfection, different explant materials is placed can carry out follow -up disinfection operation on the filter screen, and the loss of explant material is not worried to the accessible tap antiseptic solution of draining in disinfection back, again can be conveniently takes out the explant from the filter screen, brings very big facility for experiment operation.
Description
Technical field
The utility model relates to Plant Tissue Breeding equipment technical field, especially a kind of Plant Tissue Breeding Explant sterilizing device.
Background technology
Plant Tissue Breeding (planttissueculture), refer under aseptic and manually operated environmental condition, utilize suitable medium, in vitro plant organ, tissue, cell and protoplast are cultivated, make the technology of its regenerative cell or whole plant.Cultivate also known as Vitro Plant.
The key link in Plant Tissue Breeding to the sterilization of explant, traditional sterilization method is sterilized to explant with the conical flask of sterilizing or jam jar, the whole process of sterilization will use 70% alcohol, raw mercury, the sterilization such as clorox agent treated, finally also just can reach good sterilization effect with the flushing that a large amount of sterile waters is repeated multiple times, although this sterilization method can meet the basic need of existing explant sterilization, but also, there are the following problems: 1, traditional sterilization method process is very cumbersome, also SC is needed during operation, otherwise be easy to pollute or material is together poured out with sterilizing reagent and caused waste.If 2 need to sterilize to different explants in an experiment simultaneously, prepare multiple sterilizing bottle, process is more loaded down with trivial details, and workload is larger.
Summary of the invention
The purpose of this utility model is the deficiency overcoming above-mentioned prior art, provides a kind of structure simple, easy to operate and can simultaneously to the Plant Tissue Breeding Explant sterilizing device that different explants carries out disinfection.
To achieve these goals, the technical solution adopted in the utility model is: a kind of Plant Tissue Breeding Explant sterilizing device, it is characterized in that: comprise sterilizing bottle, Sterilizing cylinder, filter screen and upper cover, in sterilizing bottle and Sterilizing cylinder, middle part is installed with a filter screen respectively, the lower end of Sterilizing cylinder is connected with sterilizing bottle openend thread seal and is connected with sterilizing bottle, upper cover is fastened on the upper end of Sterilizing cylinder, be provided with leakage fluid dram in the bottom of sterilizing bottle close to bottom surface place, leakage fluid dram be provided with a tap.
Further, in described sterilizing bottle and Sterilizing cylinder, middle part is fixed with installation draw-in groove respectively, described filter screen is fixedly connected on draw-in groove.
Further, described sterilizing bottle, Sterilizing cylinder and upper cover are made by polypropylene plastics.
The beneficial effects of the utility model: structure is simple, easy to operate.By arranging sterilizing bottle and Sterilizing cylinder, can carry out disinfection to different explants simultaneously, workload when testing can be reduced, increase work efficiency; During sterilization, different explant materials is placed on filter screen can carry out follow-up sterilizing operation, to drain thimerosal, do not worry the loss of explant material after sterilization by tap, conveniently can take out explant from filter screen again, bring very large facility to experimental implementation.
During use, also by increasing the quantity of Sterilizing cylinder, and adopting the filter screen of different pore size, making the scope of application of the present utility model wider.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is described in further detail.
Fig. 1 is structural representation of the present utility model.
Embodiment
As shown in Figure 1, a kind of Plant Tissue Breeding Explant sterilizing device of the utility model, comprises sterilizing bottle 1, Sterilizing cylinder 2, filter screen 3 and upper cover 4.In sterilizing bottle 1 and Sterilizing cylinder 2, middle part is installed with a filter screen 3 respectively, the lower end of Sterilizing cylinder 2 is connected with the openend thread seal of sterilizing bottle 1, is connected after connecting between Sterilizing cylinder 2 with sterilizing bottle 1.Upper cover 4 is fastened on the upper end of Sterilizing cylinder 2, is provided with leakage fluid dram in the bottom of sterilizing bottle 1 close to bottom surface place, leakage fluid dram is provided with a tap 5.By the design that thread seal connects, sterilizing bottle 1 and Sterilizing cylinder 2 can be nested together in use, form a whole.
In the present embodiment, above-mentioned sterilizing bottle 1 and the interior middle part of Sterilizing cylinder 2 are fixed with installation draw-in groove 6 respectively, and above-mentioned filter screen 3 is fixedly connected on draw-in groove 6.
Above-mentioned sterilizing bottle 1, Sterilizing cylinder 2 and upper cover 4 are made by polypropylene plastics.
Certainly, above-mentioned Sterilizing cylinder 2 also can be provided with multiple, and in each Sterilizing cylinder, all correspondence is provided with a filter screen 3, and during use, adjacent two Sterilizing cylinders 2 head and the tail thread seal connects, and is applicable to the sterilization of multiple different explants.
Use flow process of the present utility model is: first carry out autoclave sterilization to all parts be installed together, be placed on the clean bench after ultraviolet-sterilization after sterilizing.Secondly, open upper cover 4 and sterilizing bottle 1 and Sterilizing cylinder 2 are separated, multiple explants to be sterilized are placed on the filter screen 3 of sterilizing bottle 1 and Sterilizing cylinder 2 simultaneously, be assembled into again together after completing, inject thimerosal or sterile water wash liquid from Sterilizing cylinder 2 upper end, cover upper cover 4, explant is carried out disinfection or cleans, after terminal disinfection terminates, open the tap 5 be arranged on bottom sterilizing bottle 1, waste liquid of sterilizing is discharged.
In whole disinfecting process, filter screen 3 plays buffer action, the discharge of tap 5 convenient sterilizing waste liquid, does not worry again that explant together flows out with sterilization waste liquid and causes waste simultaneously; After waste liquid is discharged, then sterilizing bottle 1 and Sterilizing cylinder 2 are separated, explant can be taken out from filter screen 3.Whole disinfecting process is easy to operate, brings very large facility to experimenter.
Finally should be noted that; above content is only in order to illustrate the technical solution of the utility model; but not the restriction to the utility model protection domain; the simple modification that those of ordinary skill in the art carries out the technical solution of the utility model or equivalently to replace, does not all depart from essence and the scope of technical solutions of the utility model.
Claims (3)
1. a Plant Tissue Breeding Explant sterilizing device, it is characterized in that: comprise sterilizing bottle, Sterilizing cylinder, filter screen and upper cover, in sterilizing bottle and Sterilizing cylinder, middle part is installed with a filter screen respectively, the lower end of Sterilizing cylinder is connected with sterilizing bottle openend thread seal and is connected with sterilizing bottle, upper cover is fastened on the upper end of Sterilizing cylinder, be provided with leakage fluid dram in the bottom of sterilizing bottle close to bottom surface place, leakage fluid dram be provided with a tap.
2. Plant Tissue Breeding Explant sterilizing device according to claim 1, is characterized in that: in described sterilizing bottle and Sterilizing cylinder, middle part is fixed with installation draw-in groove respectively, described filter screen is fixedly connected on draw-in groove.
3. Plant Tissue Breeding Explant sterilizing device according to claim 1 and 2, is characterised in that: described sterilizing bottle, Sterilizing cylinder and upper cover are made by polypropylene plastics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520608485.2U CN204888280U (en) | 2015-08-13 | 2015-08-13 | Plant tissue culture explant degassing unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520608485.2U CN204888280U (en) | 2015-08-13 | 2015-08-13 | Plant tissue culture explant degassing unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204888280U true CN204888280U (en) | 2015-12-23 |
Family
ID=54910520
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520608485.2U Expired - Fee Related CN204888280U (en) | 2015-08-13 | 2015-08-13 | Plant tissue culture explant degassing unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204888280U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106888976A (en) * | 2017-04-01 | 2017-06-27 | 中国农业科学院草原研究所 | Plant Tissue Breeding Explant sterilizing device |
| CN107347640A (en) * | 2017-07-10 | 2017-11-17 | 扬州大学 | The chlorination equipment and method of a kind of plant tissue culture course |
| CN107455260A (en) * | 2017-09-13 | 2017-12-12 | 西南大学 | Xylophyta explant stem section sterilizer |
| CN108096610A (en) * | 2018-03-06 | 2018-06-01 | 王意 | A kind of chlorination equipment and its application method of the experiment appliance for being used to handle tainting virus |
| CN108668895A (en) * | 2018-04-24 | 2018-10-19 | 贵州大学 | A kind of tissue cultures explant flusher and its application method |
| CN108935102A (en) * | 2018-08-15 | 2018-12-07 | 扬州大学 | A kind of Plant Tissue Breeding easily floats Explant sterilizing device and its sterilization method |
-
2015
- 2015-08-13 CN CN201520608485.2U patent/CN204888280U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106888976A (en) * | 2017-04-01 | 2017-06-27 | 中国农业科学院草原研究所 | Plant Tissue Breeding Explant sterilizing device |
| CN107347640A (en) * | 2017-07-10 | 2017-11-17 | 扬州大学 | The chlorination equipment and method of a kind of plant tissue culture course |
| CN107455260A (en) * | 2017-09-13 | 2017-12-12 | 西南大学 | Xylophyta explant stem section sterilizer |
| CN108096610A (en) * | 2018-03-06 | 2018-06-01 | 王意 | A kind of chlorination equipment and its application method of the experiment appliance for being used to handle tainting virus |
| CN108668895A (en) * | 2018-04-24 | 2018-10-19 | 贵州大学 | A kind of tissue cultures explant flusher and its application method |
| CN108935102A (en) * | 2018-08-15 | 2018-12-07 | 扬州大学 | A kind of Plant Tissue Breeding easily floats Explant sterilizing device and its sterilization method |
| CN108935102B (en) * | 2018-08-15 | 2022-03-04 | 扬州大学 | Plant tissue culture and easy-floating explant disinfection device and disinfection method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151223 Termination date: 20160813 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |