CN108935102B - Plant tissue culture and easy-floating explant disinfection device and disinfection method thereof - Google Patents
Plant tissue culture and easy-floating explant disinfection device and disinfection method thereof Download PDFInfo
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- CN108935102B CN108935102B CN201810927735.7A CN201810927735A CN108935102B CN 108935102 B CN108935102 B CN 108935102B CN 201810927735 A CN201810927735 A CN 201810927735A CN 108935102 B CN108935102 B CN 108935102B
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention relates to a plant tissue culture and easily floats explant degassing unit and its sterilization method, including screen cylinder, seal closure, screen cylinder have several mesh, one end opening of screen cylinder, seal closure cover in the opening of screen cylinder; a connecting clamp is arranged on one side of the opening of the screen cylinder, the sealing cover is hinged to the connecting clamp, an adjustable handle is fixed on the connecting clamp, and the adjustable handle is perpendicular to the opening of the screen cylinder; the adjustable handle is provided with a sliding chute, an adjustable button is arranged in the sliding chute, and the adjustable button slides up and down along the sliding chute; the sealing cover is characterized by further comprising a connecting handle, one end of the connecting handle is fixedly connected with the adjustable button, the other end of the connecting handle is arranged on the upper portion of the sealing cover, and one end of the connecting handle, which is fixedly connected with the adjustable button, is higher than the other end of the connecting handle. By the method, the explants are ensured to be completely contacted with the disinfectant, and can be fully dehydrated after the disinfection is finished, so that the problem of difficult dehydration of the tiny explants is solved, the completeness of the explants is ensured, and the disinfection efficiency of the explants is improved.
Description
Technical Field
The invention relates to a disinfection device and a disinfection method for an explant easy to float in plant tissue culture, belongs to the field of plant tissue culture, and is suitable for the disinfection and dehydration process of the explant and the disinfection and dehydration process of a microminiature explant and an explant easy to float.
Background
The plant tissue culture is a technology developed on the basis of cell totipotency, and the main operation process is that explants of plant organ tissues or cells and the like are separated from donor plants, sterilized, inoculated to a proper culture medium, dedifferentiated and differentiated under artificially created aseptic conditions, and developed into complete plants. The explant used in the explant disinfection process mainly comprises roots, stems, leaves, flowers, buds, fruits, seeds and the like of plants.
The explants collected from nature or indoors are all provided with various fungi, bacteria, viruses and other microorganisms, and if the explants are not disinfected, the cultured materials in the tissue culture process are polluted and the normal growth of the tissue culture materials is influenced, so the disinfection treatment of the explants is the basic premise and guarantee of the success of tissue culture. In the disinfection process, the completeness of the explant needs to be guaranteed and secondary pollution needs to be avoided on the premise of thorough disinfection. In the disinfection process, a plurality of explants are easy to float, the incomplete disinfection is easy to occur, and the possibility of secondary pollution caused by inconvenient dehydration is easy to occur for the disinfection of the microminiature explants.
Disclosure of Invention
The invention aims to solve the problems, provides a disinfection device for an explant easy to float in plant tissue culture and a disinfection method thereof, is applied to the explant disinfection process, is a simple and convenient operation method to improve the disinfection efficiency, and can open and close a screen cylinder to reduce the damage of the explant in the disinfection process.
The invention aims to realize the disinfection device for the easily floating explants for plant tissue culture, which is characterized by comprising a screen cylinder and a sealing cover, wherein a plurality of screen holes are arranged on the screen cylinder; a connecting clamp is arranged on one side of the opening of the screen cylinder, the sealing cover is hinged to the connecting clamp, an adjustable handle is fixed on the connecting clamp, and the adjustable handle is perpendicular to the opening of the screen cylinder;
the adjustable handle is provided with a sliding chute, an adjustable button is arranged in the sliding chute, and the adjustable button slides up and down along the sliding chute;
the sealing cover is also provided with a connecting handle, one end of the connecting handle is fixedly connected with the adjustable button, the other end of the connecting handle is arranged on the upper part of the sealing cover, and the end of the connecting handle fixedly connected with the adjustable button is higher than the other end of the connecting handle; the adjustable button slides up and down along the sliding chute to drive one end of the connecting handle which is not fixedly connected with the adjustable button to extrude the sealing cover or release the extrusion on the sealing cover; when the sealing cover is extruded by one end of the connecting handle which is not fixedly connected with the adjustable button, the sealing cover tightly covers the opening of the screen cylinder; when the end of the connecting handle which is not fixedly connected with the adjustable button releases the extrusion to the sealing cover, the sealing cover can be opened from the opening of the screen cylinder.
The sealing cover is provided with an annular groove on one side facing the opening of the screen drum, and when the sealing cover covers the opening of the screen drum, the top drum wall of the screen drum at the opening of the screen drum is arranged in the annular groove, so that the sealing cover and the opening of the screen drum form an airtight structure, and explants are prevented from flowing out.
A plurality of water permeable holes are distributed on the sealing cover, and the size of each water permeable hole is 40 meshes so as to keep water flow smooth and prevent explants from flowing out.
The spout runs through adjustable handle opposite side from adjustable handle one side.
The nut is screwed on the adjustable knob and is arranged outside the chute on the adjustable handle, when the nut is screwed along the adjustable knob towards the direction of the adjustable handle, the nut is tightly attached to the adjustable handle, and the adjustable knob is fixed in the chute on the adjustable handle; when the nut is screwed along the adjustable button in the direction back to the adjustable handle, the nut is not attached to the adjustable handle, and the adjustable button can slide up and down along the sliding groove on the adjustable handle.
The mesh on the screen cylinder is smaller than the size of the explant.
A method for disinfecting explants by using a plant tissue culture floatable explant disinfection device is characterized by comprising the following steps:
step 1), putting the explant to be disinfected into a disinfection device, firstly adjusting an adjustable button on an adjustable handle to the highest position of a chute in the chute of the adjustable handle, opening a sealing cover, and putting the explant to be disinfected into a screen cylinder from an opening of the screen cylinder; then the sealing cover is closed, the adjustable button is adjusted to the lowest position of the sliding chute of the adjustable handle, the adjustable button is fixed at the lowest position of the sliding chute of the adjustable handle, one end of the connecting handle, which is not fixedly connected with the adjustable button, extrudes the sealing cover, the sealing cover tightly covers the opening of the screen cylinder, the sealing cover is tightly combined with the screen cylinder, and explants in the screen cylinder are prevented from flowing out; putting the explants to be disinfected into a disinfection device;
step 2), placing a first beaker, a second beaker, a third beaker, a fourth beaker, a fifth beaker and a sixth beaker on a clean bench, pouring 70-75% of alcohol into the first beaker for standby, pouring sterile water into the second beaker for standby, pouring 1% of sodium hypochlorite solution into the third beaker for standby, and pouring sterile water into the fourth beaker, the fifth beaker and the sixth beaker for standby; placing the disinfection device which is placed with the explants to be disinfected in the step 1) under running water for washing for 1-2 hours, and then placing the disinfection device on a superclean workbench for standby;
step 3), placing the disinfection device on a superclean workbench, placing the disinfection device in a first beaker on the superclean workbench, submerging the disinfection device in 70-75% alcohol in the first beaker, stirring and soaking for 10-20s, and taking out the disinfection device, wherein in the stirring and soaking process of the disinfection device, alcohol enters a screen cylinder from a plurality of screen holes on the screen cylinder, so that explants in the screen cylinder are stirred and soaked in the alcohol;
then the sterilizing device is transferred into a second beaker, and is stirred and soaked after being submerged in the sterile water in the second beaker, and in the stirring and soaking process of the sterilizing device, the sterile water in the second beaker enters the screen cylinder from a plurality of screen holes on the screen cylinder, so that the explants in the screen cylinder are stirred and soaked in the sterile water in the second beaker;
then, transferring the disinfection device into a third beaker, immersing the disinfection device in a 1% sodium hypochlorite solution in the third beaker, stirring and soaking for 10-20min, and taking out the disinfection device, wherein in the stirring and soaking process of the disinfection device, the 1% sodium hypochlorite solution in the third beaker enters the screen cylinder from a plurality of screen holes on the screen cylinder, so that the explants in the screen cylinder are stirred and soaked in the 1% sodium hypochlorite solution in the third beaker;
then the sterilizing device is sequentially transferred into a fourth beaker, the fourth beaker is immersed in sterile water and then stirred and soaked, a fifth beaker is immersed in sterile water and then stirred and soaked, and the sixth beaker is immersed in sterile water and then stirred and soaked;
step 4), draining water from the disinfection device treated in the step 5), wiping the surface of the disinfection device with sterile filter paper, adjusting the adjustable button to the highest position of the chute in the chute of the adjustable handle, opening the sealing cover, and taking out the explant to a culture dish containing the sterile filter paper with tweezers;
and 5) absorbing water in a culture dish of sterile filter paper to obtain the sterilized explant.
The invention has reasonable structure, easy production and manufacture and convenient use, and the specific technical scheme of the invention is as follows: a plant tissue culture and easy-floating explant disinfection device comprises a screen cylinder and a sealing cover, wherein a plurality of screen holes are formed in the screen cylinder, one end of the screen cylinder is opened, and the sealing cover covers the opening of the screen cylinder; a connecting clamp is arranged on one side of an opening of the screen cylinder, the sealing cover is hinged to the connecting clamp, an adjustable handle is fixed on the connecting clamp, and the adjustable handle is perpendicular to the opening of the screen cylinder; the adjustable handle is provided with a chute, an adjustable button is arranged in the chute, and the adjustable button slides up and down along the chute; one end of the connecting handle is fixedly connected with the adjustable button, the other end of the connecting handle is arranged on the upper portion of the sealing cover, and one end of the connecting handle fixedly connected with the adjustable button is higher than the other end of the connecting handle.
When the adjustable button is used, the adjustable button slides up and down along the sliding chute to drive one end of the connecting handle which is not fixedly connected with the adjustable button to extrude the sealing cover or release the extrusion on the sealing cover; when the sealing cover is extruded by one end of the connecting handle which is not fixedly connected with the adjustable button, the sealing cover tightly covers the opening of the screen cylinder; when the end of the connecting handle which is not fixedly connected with the adjustable button releases the extrusion to the sealing cover, the sealing cover can be opened from the opening of the screen cylinder.
The whole device is made of stainless steel, and is not easy to damage in the disinfection process. Furthermore, a groove is arranged below the sealing cover and can be tightly combined with the screen cylinder. Furthermore, the mesh size of the screen cylinder can select screen cylinders with different meshes for explant disinfection according to the size of the explant, so that the highest disinfection efficiency is ensured. The sealing cover, the adjustable handle and the screen cylinder are connected through a connecting clamp.
Compared with the prior art, the disinfection device for the plant tissue culture explant easy to float has the following advantages:
simple structure, convenient operation, through the connecting handle not with adjustable button fixed connection's one end extrusion seal closure, the seal closure tightly covers in the opening of a screen cloth section of thick bamboo, can make a screen cloth section of thick bamboo form airtight space, prevents that microminiature and easy showy explant from flowing out, guarantees explant complete contact antiseptic solution, can fully dewater after the disinfection, avoids small explant dehydration difficulty problem again to guarantee the integrality of explant and improve explant disinfection efficiency.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic top view of a screen cylinder according to the present invention.
Fig. 3 is a schematic view of the bottom view of the sealing cap of the present invention.
Fig. 4 is a schematic structural view of an adjustable handle according to the present invention.
Fig. 5 is a schematic view of a screen cylinder structure according to the present invention.
Fig. 6 is a schematic top view of the closure of the present invention.
In the figure: 1 adjustable button, 2 adjustable handle, 3 joint card, 4 connecting handles, 5 seal closures, 6 screen mesh section of thick bamboo, 7 sieve meshes, 8 holes of permeating water.
Detailed Description
The invention is further described with reference to the accompanying drawings and the description thereof.
A plant tissue culture explant disinfection device easy to float comprises a screen drum 6 and a sealing cover 5, wherein a plurality of screen holes 7 are formed in the screen drum 6, one end of the screen drum 6 is opened, and the sealing cover 5 covers the opening of the screen drum 6; the opening one side of a screen cloth section of thick bamboo 6 sets up joint card 3, and 5 hinged joint of closing cap are on joint card 3, and are fixed with adjustable handle 2 on the joint card 3, and adjustable handle 2 is perpendicular with the opening of a screen cloth section of thick bamboo 6.
A sliding groove is arranged on the adjustable handle 2, an adjustable button 1 is arranged in the sliding groove, and the adjustable button 1 slides up and down along the sliding groove; a connecting handle 4 is further arranged, one end of the connecting handle 4 is fixedly connected with the adjustable button 1, the other end of the connecting handle 4 is arranged on the upper part of the sealing cover 5, and the end of the connecting handle 4 fixedly connected with the adjustable button 1 is higher than the other end of the connecting handle 4; the adjustable button 1 slides up and down along the sliding chute to drive one end of the connecting handle 4 which is not fixedly connected with the adjustable button 1 to extrude the sealing cover 5 or release the extrusion on the sealing cover 5; when one end of the connecting handle 4, which is not fixedly connected with the adjustable button 1, extrudes the sealing cover 5, the sealing cover 5 tightly covers the opening of the screen cylinder 6; when the end of the connecting handle 4 not fixedly connected with the adjustable button 1 releases the extrusion to the sealing cover 5, the sealing cover 5 can be opened from the opening of the screen cylinder 6.
Set up the annular groove on the face of seal closure 5 towards screen drum 6 opening, when seal closure 5 lid was in screen drum 6 opening, screen drum top section of thick bamboo wall at screen drum 6 opening part all arranged in the annular groove for seal closure 5 and screen drum 6 mouth formation are airtight, prevent that the explant from flowing out. A plurality of water permeable holes 8 are distributed on the sealing cover 5, and the size of each water permeable hole is 40 meshes so as to keep the water flow smooth and prevent the explant from flowing out. The spout runs through adjustable handle 2 opposite side from adjustable handle 2 one side.
A nut is screwed on the adjustable knob 1 and is arranged outside a chute on the adjustable handle 2, when the nut is screwed along the adjustable knob 1 towards the direction of the adjustable handle 2, the nut is tightly attached to the adjustable handle 2, and the adjustable knob 1 is fixed in the chute on the adjustable handle 2; when the nut is screwed along the direction of the adjustable knob 1 back to the adjustable handle 2, the nut is not attached to the adjustable handle 2, and the adjustable knob 1 can slide up and down along the sliding groove on the adjustable handle 2.
When the plant tissue culture floatable explant sterilization device is used for explant sterilization, the method comprises the following steps:
step 1), putting the explant to be disinfected into a disinfection device, firstly adjusting an adjustable button 1 on an adjustable handle 2 to the highest position of a chute in the chute of the adjustable handle 2, opening a sealing cover 5, and putting the explant to be disinfected into a screen cylinder 6 from an opening of the screen cylinder 6; then, the sealing cover 5 is closed, the adjustable button 1 is adjusted to the lowest position of the sliding chute of the adjustable handle 2, the adjustable button 1 is fixed at the lowest position of the sliding chute of the adjustable handle 2, one end of the connecting handle 4, which is not fixedly connected with the adjustable button 1, extrudes the sealing cover 5, the sealing cover 5 tightly covers the opening of the screen cylinder 6, the sealing cover 5 is tightly combined with the screen cylinder 6, the screen holes 7 on the screen cylinder 6 are smaller than the size of explants, and the explants in the screen cylinder 6 are prevented from flowing out; putting the explants to be disinfected into a disinfection device;
step 2), placing a first beaker, a second beaker, a third beaker, a fourth beaker, a fifth beaker and a sixth beaker on a clean bench, pouring 70-75% of alcohol into the first beaker for standby, pouring sterile water into the second beaker for standby, pouring 1% of sodium hypochlorite solution into the third beaker for standby, and pouring sterile water into the fourth beaker, the fifth beaker and the sixth beaker for standby; placing the disinfection device which is placed with the explants to be disinfected in the step 1) under running water for washing for 1-2 hours, and then placing the disinfection device on a superclean workbench for standby;
step 3), placing the disinfection device on a superclean workbench, placing the disinfection device in a first beaker on the superclean workbench, submerging the disinfection device in 70-75% alcohol in the first beaker, stirring and soaking for 10-20s, and taking out the disinfection device, wherein in the stirring and soaking process of the disinfection device, alcohol enters a screen cylinder 6 from a plurality of screen holes 7 on the screen cylinder 6, so that explants in the screen cylinder 6 are stirred and soaked in the alcohol;
then the sterilizing device is transferred into a second beaker, and is soaked in the sterile water in the second beaker in a stirring manner, and in the stirring and soaking process of the sterilizing device, the sterile water in the second beaker enters the screen cylinder 6 from the plurality of screen holes 7 on the screen cylinder 6, so that the explants in the screen cylinder 6 are soaked in the sterile water in the second beaker in a stirring manner;
then, the disinfection device is transferred into a third beaker, the disinfection device is submerged in a 1% sodium hypochlorite solution in the third beaker, stirred and soaked for 10-20min and then taken out, and in the stirring and soaking process of the disinfection device, the 1% sodium hypochlorite solution in the third beaker enters the screen cylinder 6 from the plurality of screen holes 7 on the screen cylinder 6, so that explants in the screen cylinder 6 are stirred and soaked in the 1% sodium hypochlorite solution in the third beaker;
then the sterilizing device is sequentially transferred into a fourth beaker, the fourth beaker is immersed in sterile water and then stirred and soaked, a fifth beaker is immersed in sterile water and then stirred and soaked, and a sixth beaker is immersed in sterile water and then stirred and soaked, when the sterilizing device is arranged in the fourth beaker, the fifth beaker and the sixth beaker, the sterile water in the fourth beaker, the fifth beaker and the sixth beaker enters a screen cylinder 6 from a plurality of screen holes 7 on the screen cylinder 6, so that explants in the screen cylinder 6 are respectively and sequentially immersed in the sterile water in the fourth beaker, stirred and soaked, the sterile water in the fifth beaker is immersed and then stirred and soaked, and the sterile water in the sixth beaker is immersed and then stirred and soaked, and the sterilizing device is cleaned by using sterile water;
step 4), draining water from the disinfection device treated in the step 5), wiping the surface of the disinfection device with sterile filter paper, adjusting the adjustable button 1 to the highest position of the sliding chute in the sliding chute of the adjustable handle 2, opening the sealing cover 5, and taking out the explant to a culture dish containing the sterile filter paper with tweezers;
and 5) absorbing water in a culture dish of sterile filter paper to obtain the sterilized explant. Because the sterilizing device is small in size, a plurality of sterilizing effects can be achieved in a 1000ml beaker at one time, so that the sterilizing efficiency is improved.
In addition, because the disinfection device mainly disinfects microminiature explants, the size of the disinfection device is small, a plurality of disinfection devices filled with explants can be put into a big beaker for disinfection during disinfection, the disinfection efficiency is improved, and a plurality of disinfection effects can be achieved at one time.
The invention has simple structure and convenient operation, can prevent the loss of explants in the disinfection process and overcome the problems of difficult dehydration of the explants and the like.
Claims (3)
1. A plant tissue culture explant disinfection device easy to float is characterized by comprising a screen drum (6) and a sealing cover (5), wherein a plurality of screen holes (7) are formed in the screen drum (6), one end of the screen drum (6) is opened, and the sealing cover (5) covers the opening of the screen drum (6); a connecting clamp (3) is arranged on one side of an opening of the screen cylinder (6), the sealing cover (5) is hinged to the connecting clamp (3), an adjustable handle (2) is fixed on the connecting clamp (3), and the adjustable handle (2) is perpendicular to the opening of the screen cylinder (6);
the adjustable handle (2) is provided with a sliding chute, an adjustable button (1) is arranged in the sliding chute, and the adjustable button (1) slides up and down along the sliding chute;
the sealing cap is also provided with a connecting handle (4), one end of the connecting handle (4) is fixedly connected with the adjustable button (1), the other end of the connecting handle (4) is arranged at the upper part of the sealing cap (5), and one end of the connecting handle (4) fixedly connected with the adjustable button (1) is higher than the other end of the connecting handle (4); the adjustable button (1) slides up and down along the sliding chute to drive one end of the connecting handle (4) which is not fixedly connected with the adjustable button (1) to extrude the sealing cover (5) or release the extrusion on the sealing cover (5); when one end of the connecting handle (4), which is not fixedly connected with the adjustable button (1), extrudes the sealing cover (5), the sealing cover (5) tightly covers the opening of the screen cylinder (6); when one end of the connecting handle (4), which is not fixedly connected with the adjustable button (1), releases to press the sealing cover (5), the sealing cover (5) can be opened from the opening of the screen cylinder (6);
an annular groove is formed in one surface, facing the opening of the screen cylinder (6), of the sealing cover (5), when the sealing cover (5) covers the opening of the screen cylinder (6), the cylinder wall of the top of the screen cylinder at the opening of the screen cylinder (6) is arranged in the annular groove, so that the sealing cover (5) and the opening of the screen cylinder (6) are sealed, and explants are prevented from flowing out;
a nut is screwed on the adjustable button (1), the nut is arranged outside a sliding groove on the adjustable handle (2), when the nut is screwed along the adjustable button (1) towards the direction of the adjustable handle (2), the nut is tightly attached to the adjustable handle (2), and the adjustable button (1) is fixed in the sliding groove on the adjustable handle (2); when the nut is screwed along the adjustable knob (1) in the direction back to the adjustable handle (2), the nut is not attached to the adjustable handle (2), and the adjustable knob (1) can slide up and down along a sliding groove on the adjustable handle (2);
the sliding chute penetrates through the other side of the adjustable handle (2) from one side of the adjustable handle (2); a plurality of water permeable holes (8) are distributed on the sealing cover (5), and the size of each water permeable hole is 40 meshes so as to keep water flow smooth and prevent explants from flowing out.
2. A plant tissue culture floatable explant sterilization device according to claim 1, wherein said screen cylinder (6) has screen holes (7) smaller than the size of the explant.
3. The method for disinfecting explants by using the device for disinfecting the plant tissue culture floatable explants according to any one of the claims 1 to 2, which is characterized by comprising the following steps:
step 1), putting explants to be disinfected into a disinfection device, firstly adjusting an adjustable button (1) on an adjustable handle (2) to the highest position of a chute in the chute of the adjustable handle (2), opening a sealing cover (5), and putting the explants to be disinfected into a screen cylinder (6) from an opening of the screen cylinder (6); then closing the sealing cover (5), adjusting the adjustable button (1) to the lowest position of the sliding chute of the adjustable handle (2), fixing the adjustable button (1) at the lowest position of the sliding chute of the adjustable handle (2), extruding the sealing cover (5) by one end of the connecting handle (4) which is not fixedly connected with the adjustable button (1), and tightly covering the opening of the screen cylinder (6) with the sealing cover (5) so that the sealing cover (5) is tightly combined with the screen cylinder (6) to prevent explants in the screen cylinder (6) from flowing out; putting the explants to be disinfected into a disinfection device;
step 2), placing a first beaker, a second beaker, a third beaker, a fourth beaker, a fifth beaker and a sixth beaker on a clean bench, pouring 70-75% of alcohol into the first beaker for standby, pouring sterile water into the second beaker for standby, pouring 1% of sodium hypochlorite solution into the third beaker for standby, and pouring sterile water into the fourth beaker, the fifth beaker and the sixth beaker for standby; placing the disinfection device which is placed with the explants to be disinfected in the step 1) under running water for washing for 1-2 hours, and then placing the disinfection device on a superclean workbench for standby;
step 3), placing the disinfection device in a first beaker on a super-clean workbench, submerging the disinfection device in 70-75% alcohol in the first beaker, stirring and soaking for 10-20s, and taking out the disinfection device, wherein in the stirring and soaking process of the disinfection device, the alcohol enters a screen cylinder (6) from a plurality of screen holes (7) on the screen cylinder (6), so that explants in the screen cylinder (6) are stirred and soaked in the alcohol;
then the sterilizing device is transferred into a second beaker, and is stirred and soaked after being submerged in the sterile water in the second beaker, and in the stirring and soaking process of the sterilizing device, the sterile water in the second beaker enters the screen cylinder (6) from a plurality of screen holes (7) on the screen cylinder (6), so that the explants in the screen cylinder (6) are stirred and soaked in the sterile water in the second beaker;
then, the disinfection device is transferred into a third beaker, the third beaker is immersed in a 1% sodium hypochlorite solution and then stirred and soaked for 10-20min, and then the third beaker is taken out, in the stirring and soaking process of the disinfection device, the 1% sodium hypochlorite solution in the third beaker enters the screen cylinder (6) from a plurality of screen holes (7) on the screen cylinder (6), so that explants in the screen cylinder (6) are stirred and soaked in the 1% sodium hypochlorite solution in the third beaker;
then the sterilizing device is sequentially transferred into a fourth beaker, the fourth beaker is immersed in sterile water and then stirred and soaked, a fifth beaker is immersed in sterile water and then stirred and soaked, and the sixth beaker is immersed in sterile water and then stirred and soaked, when the sterilizing device is arranged in the fourth beaker, the fifth beaker and the sixth beaker, the sterile water in the fourth beaker, the fifth beaker and the sixth beaker enters a screen cylinder (6) from a plurality of screen holes (7) on the screen cylinder (6), so that explants in the screen cylinder (6) are respectively and sequentially immersed in the sterile water in the fourth beaker and then stirred and soaked, the sterile water in the fifth beaker is immersed and then stirred and soaked, and the sterile water in the sixth beaker is then stirred and soaked, so that the sterilizing device is cleaned by using the sterile water;
step 4), draining water of the disinfection device treated in the step 5), wiping the surface of the disinfection device with sterile filter paper, adjusting the adjustable button (1) to the highest position of the sliding chute in the sliding chute of the adjustable handle (2), opening the sealing cover (5), and taking out the explant to a culture dish containing the sterile filter paper with tweezers;
and 5) absorbing water in a culture dish of sterile filter paper to obtain the sterilized explant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810927735.7A CN108935102B (en) | 2018-08-15 | 2018-08-15 | Plant tissue culture and easy-floating explant disinfection device and disinfection method thereof |
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| CN201810927735.7A CN108935102B (en) | 2018-08-15 | 2018-08-15 | Plant tissue culture and easy-floating explant disinfection device and disinfection method thereof |
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| CN108935102A CN108935102A (en) | 2018-12-07 |
| CN108935102B true CN108935102B (en) | 2022-03-04 |
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| CN109349112A (en) * | 2018-11-29 | 2019-02-19 | 山东省果树研究所 | A kind of explant locellus disinfection lost circulation prevention device and digital display time control speed regulation cyclotron oscillation device and its application |
| CN111226795B (en) * | 2020-03-06 | 2022-12-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
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| JP3492684B2 (en) * | 1992-04-24 | 2004-02-03 | シー ジュニア ミッテル，ジョゼフ | Lid device |
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