CN111226795B - Sterilization device for floating samples - Google Patents
Sterilization device for floating samples Download PDFInfo
- Publication number
- CN111226795B CN111226795B CN202010151969.4A CN202010151969A CN111226795B CN 111226795 B CN111226795 B CN 111226795B CN 202010151969 A CN202010151969 A CN 202010151969A CN 111226795 B CN111226795 B CN 111226795B
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- Prior art keywords
- steel wire
- baffle
- elastic steel
- floating
- bottle
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 38
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 31
- 241000209490 Nymphaea Species 0.000 claims abstract description 24
- 235000016791 Nymphaea odorata subsp odorata Nutrition 0.000 claims abstract description 24
- 229910000831 Steel Inorganic materials 0.000 claims abstract description 22
- 239000010959 steel Substances 0.000 claims abstract description 22
- 239000011148 porous material Substances 0.000 claims abstract description 6
- 239000011521 glass Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 206010052428 Wound Diseases 0.000 description 5
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 241000209477 Nymphaeaceae Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005489 elastic deformation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a sterilization device for a floating sample, which comprises a tissue culture bottle, a bottle cap, a connecting pipe, a fixing pipe, a baffle, a fixture block and an elastic steel wire. Wherein the bottle cap is in threaded connection with the bottle mouth of the tissue culture bottle; the upper end of the connecting pipe is fixedly connected with the bottle cap, and a plurality of clamping groove holes are formed in the connecting pipe along the length direction; the upper end of the fixed pipe is inserted into the connecting pipe, and a pore channel is arranged on the fixed pipe along the radial direction; the baffle is provided with a plurality of through holes, and the lower end of the fixed pipe is fixedly connected with the baffle; the clamping block is arranged in the pore channel in a sliding manner, and the diameter of the clamping groove hole is not smaller than that of the pore channel; one end of the elastic steel wire is inserted into the clamping block, and the other end of the elastic steel wire is abutted against the inner wall of the fixed pipe. The height of the baffle is adjusted by changing the position of the clamping groove hole where the clamping block is located, so that the water lily leaves are always soaked below the liquid level of the sterilization solution, the waste of the sterilization agent is reduced, the environmental pollution is reduced, and the detachable connection mode is adopted, so that the water lily leaves are convenient to clean and store.
Description
Technical Field
The invention relates to the technical field of plant tissue culture, in particular to a sterilization device for a floating sample.
Background
Nymphaeaceae (Nymphaeaceae) Nymphaeaceae (Nymphaeacalin.) plants are perennial root herbaceous plants, and can be widely applied to the fields of landscape architecture, medicine, food, daily chemical industry and the like due to higher ornamental, edible and medicinal values. With the opening and circulation of international flower markets, new varieties and varieties of the tropical water lily are continuously introduced into China in recent years, and good variety breeding and large-scale planting of the tropical water lily are successively carried out in provinces and cities such as Hainan province, fujian province, guangzhou province, suzhou province, hangzhou province, qingdao province and the like, so that the water lily industrialization shows an expanding development trend.
In the field cultivation of water lily, a plant division or sowing propagation mode is usually adopted, but the plant division propagation consumes long time, the number of obtained asexual propagation new plants is limited, and the natural asexual propagation capacity of part of varieties is weak; in addition, depending on the sowing and propagation mode of the seeds, the obtained plants have large quality difference and are not beneficial to standardized and normalized production.
The application of aseptic seedlings has become an important component of agricultural standardized and large-scale production. The large-scale and standardized development of the water lily industry can be effectively promoted by developing the aseptic seedling research of the water lily excellent varieties. The explants such as leaves, tubers and the like of the water lily contain phenolic substances, the cut wounds of the water lily are easy to brown when the water lily is exposed in the air, the explants such as leaves and the like are easy to float out of the surface of a sterilization solution in the sterilization process of the explants, and the wounds of the explants floating on the surface are browned due to contact with the air, so that the induction rate of subsequent aseptic seedlings is reduced.
Therefore, how to ensure that the water lily leaves are completely soaked in the solution in the sterilization process and reduce the time of exposing the wound of the explant to the air is a technical problem to be solved urgently by the technical personnel in the field.
Disclosure of Invention
The invention aims to provide a sterilization device for a floating sample, which is used for solving the technical problems in the prior art, ensuring that water lily leaves are completely soaked in a solution in the sterilization process and reducing the time of exposing wounds of explants to the air.
In order to achieve the purpose, the invention provides the following scheme:
the invention discloses a sterilization device for a floating sample, which comprises:
the tissue culture bottle is used for containing water lily leaves and a sterilization solution;
the bottle cap is in threaded connection with the bottle mouth of the tissue culture bottle;
the upper end of the connecting pipe is fixedly connected with the bottle cap, and a plurality of clamping groove holes are formed in the connecting pipe along the length direction;
the upper end of the fixed pipe is inserted into the connecting pipe, and a pore channel is arranged on the fixed pipe along the radial direction;
the baffle plate is provided with a plurality of through holes, and the lower end of the fixed pipe is fixedly connected with the baffle plate;
the clamping block is arranged in the duct in a sliding mode, and the diameter of the clamping groove hole is not smaller than that of the duct;
the elastic steel wire, the one end of elastic steel wire inserts in the fixture block, the other end of elastic steel wire with the inner wall of fixed pipe offsets, the elastic steel wire is located the outside part of fixture block wholly is the U-shaped structure.
Preferably, the lower surface of the bottle cap has a threaded hole, and the upper end of the connecting pipe is in threaded connection with the threaded hole.
Preferably, the lower end of the fixing tube is threadedly coupled to the baffle plate.
Preferably, the diameter of the elastic steel wire is 0.8mm.
Preferably, the diameter of the through hole is 1mm.
Preferably, the tissue culture bottle is made of transparent glass.
Preferably, the bottle cap, the connecting pipe, the fixing pipe, the baffle and the fixture block are made of plastic materials.
Preferably, the diameter of the snap slot hole is equal to the diameter of the duct.
Preferably, one end of the fixture block, which is positioned in the fixing tube, is provided with a limiting flange.
Compared with the prior art, the invention has the following technical effects:
the water lily leaves are always soaked below the liquid level of the sterilization solution by changing the position of the clamping groove hole where the clamping block is located and adjusting the height of the baffle, so that the water lily leaves are convenient to use a proper amount of sterilization agent in the sterilization process of the water lily leaves, the waste of the sterilization agent is reduced on one hand, most sterilization agents are toxic reagents on the other hand, the dosage can be reduced, and the environmental pollution is reduced. Through adopting the mode of can dismantling the connection, conveniently wash and deposit.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view showing the overall structure of a sterilization apparatus for floating samples according to the present embodiment;
FIG. 2 is a schematic view of a part of the structure of the sterilization apparatus for floating samples in this embodiment
FIG. 3 is a schematic view of the connection between the fixture block and the elastic steel wire;
description of reference numerals: 1, tissue culture bottle; 2, bottle cap; 3, connecting pipes; 4 fixing the tube; 5, a baffle plate; 6, clamping blocks; 7, an elastic steel wire; 8 slotted hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a sterilization device for a floating sample, which is used for solving the technical problems in the prior art, ensuring that water lily leaves are completely soaked in a solution in the sterilization process and reducing the time of exposing wounds of explants to the air.
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description thereof.
As shown in fig. 1-3, the present embodiment provides a sterilization apparatus for floating samples, which includes a tissue culture bottle 1, a bottle cap 2, a connecting tube 3, a fixing tube 4, a baffle 5, a fixture block 6, and an elastic steel wire 7.
Wherein, the tissue culture bottle 1 is used for containing the water lily leaves and a sterilization solution and is used as a container for sterilizing the water lily leaves. The sterilizing solution may be selected as desired, for example, a 0.1% mercuric chloride solution. The bottle cap 2 is connected with the bottle mouth of the tissue culture bottle 1 in a threaded manner. The upper end of the connecting pipe 3 is fixedly connected with the bottle cap 2, and a plurality of clamping groove holes 8 are formed in the connecting pipe 3 along the length direction. The upper end of the fixed pipe 4 is inserted into the connecting pipe 3, and a pore channel is arranged on the fixed pipe 4 along the radial direction. Baffle 5 is used for suppressing the water lily blade of its below, is equipped with a plurality of through-holes on the baffle 5, supplies the sterilization solution to flow through. The lower end of the fixed pipe 4 is fixedly connected with the baffle 5, and the height of the baffle 5 can be changed by adjusting the height of the fixed pipe 4, so that the water lily leaves are always soaked below the liquid level of the sterilization solution. The fixture block 6 is arranged in the duct in a sliding mode, and the diameter of the fixture groove hole 8 is not smaller than that of the duct, so that the fixture block 6 can freely pass through the fixture groove hole 8. One end of the elastic steel wire 7 is inserted into the fixture block 6, the other end of the elastic steel wire 7 is abutted against the inner wall of the fixed tube 4, and the part of the elastic steel wire 7, which is positioned outside the fixture block 6, is integrally in a U-shaped structure.
During the use, through inwards pressing fixture block 6, elastic steel wire 7 elastic deformation makes in fixture block 6 contracts into connecting pipe 3 through a draw-in groove hole 8, change connecting pipe 3 and fixed pipe 4 axial relative position after, can make fixture block 6 stretch out from another draw-in groove hole 8 under elastic steel wire 7's promotion to make connecting pipe 3 and fixed pipe 4 relatively fixed once more, and changed bottle lid 2, connecting pipe 3, fixed pipe 4, baffle 5, the length of this overall structure of fixture block 6 and elastic steel wire 7. Therefore, the height of the baffle 5 can be adjusted by changing the position of the clamping groove hole 8 of the clamping block 6, so that the water lily leaves are always soaked below the liquid level of the sterilization solution.
The fixed connection mode of bottle lid 2 and connecting pipe 3 has the multiple, and in this embodiment, the lower surface of bottle lid 2 has the screw hole, the upper end and the screw hole threaded connection of connecting pipe 3. Similarly, the fixing tube 4 and the baffle 5 are fixedly connected in various ways, and in this embodiment, the lower end of the fixing tube 4 is screwed with the baffle 5. Through adopting this kind of detachable connection mode of threaded connection, convenient to detach accomodates, its simple structure, convenient operation.
Specifically, in the present embodiment, the diameter of the elastic steel wire 7 is 0.8mm, and the diameter of the through hole is 1mm, and those skilled in the art can select other dimensions as needed.
In order to facilitate observation of the inside of the tissue culture bottle 1, the tissue culture bottle 1 is made of transparent glass in the embodiment.
In this embodiment, bottle lid 2, connecting pipe 3, fixed pipe 4, baffle 5 and fixture block 6 are the plastics material, and its manufacturing cost is lower, and weight is lighter to corrosion resistance is stronger.
In this embodiment, the diameter of the slot hole 8 is preferably equal to the diameter of the hole, so that the connecting tube 3 and the fixing tube 4 are more firmly clamped by the clamping block 6.
In order to prevent the clamping block 6 from coming off the fixing tube 4, a limiting flange is disposed at one end of the clamping block 6 located in the fixing tube 4.
The principle and the implementation mode of the present invention are explained by applying specific examples in the present specification, and the above descriptions of the examples are only used to help understanding the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (9)
1. A sterilization apparatus for floating a sample, comprising:
the tissue culture bottle is used for containing the water lily leaves and a sterilization solution;
the bottle cap is in threaded connection with a bottle opening of the tissue culture bottle;
the upper end of the connecting pipe is fixedly connected with the bottle cap, and a plurality of clamping groove holes are formed in the connecting pipe along the length direction;
the upper end of the fixed pipe is inserted into the connecting pipe, and a pore channel is arranged on the fixed pipe along the radial direction;
the baffle plate is provided with a plurality of through holes, and the lower end of the fixed pipe is fixedly connected with the baffle plate;
the clamping block is arranged in the duct in a sliding mode, and the diameter of the clamping groove hole is not smaller than that of the duct;
one end of the elastic steel wire is inserted into the clamping block, the other end of the elastic steel wire is abutted against the inner wall of the fixing pipe, and the part, located outside the clamping block, of the elastic steel wire is integrally in a U-shaped structure.
2. The apparatus for sterilizing floating samples according to claim 1, wherein the lower surface of the bottle cap has a screw hole, and the upper end of the connection pipe is screw-coupled to the screw hole.
3. The apparatus for sterilizing a floating sample according to claim 1, wherein a lower end of the fixing tube is screw-coupled to the baffle.
4. The apparatus for sterilizing a floating sample according to claim 1, wherein said elastic steel wire has a diameter of 0.8mm.
5. The sterilization apparatus for floating samples according to claim 1, wherein the diameter of the through hole is 1mm.
6. The sterilization apparatus for the floating sample according to claim 1, wherein the tissue culture bottle is made of transparent glass.
7. The apparatus of claim 1, wherein the cap, the connecting tube, the fixing tube, the baffle, and the fixture block are made of plastic.
8. The apparatus for sterilizing a floating sample according to claim 1, wherein a diameter of said slot hole is equal to a diameter of said tunnel.
9. The apparatus for sterilizing a floating sample according to claim 1, wherein the fixture block has a limit rib at an end thereof located in the fixing tube.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202010151969.4A CN111226795B (en) | 2020-03-06 | 2020-03-06 | Sterilization device for floating samples |
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CN202010151969.4A CN111226795B (en) | 2020-03-06 | 2020-03-06 | Sterilization device for floating samples |
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CN111226795A CN111226795A (en) | 2020-06-05 |
CN111226795B true CN111226795B (en) | 2022-12-30 |
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CN106857242A (en) * | 2015-12-11 | 2017-06-20 | 天津市华泰兰园种养殖专业合作社 | A kind of sterilization dehydration device of tissue culture explant |
CN206547542U (en) * | 2017-01-22 | 2017-10-13 | 上海市农业生物基因中心 | A kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch |
CN108935102A (en) * | 2018-08-15 | 2018-12-07 | 扬州大学 | A kind of Plant Tissue Breeding easily floats Explant sterilizing device and its sterilization method |
CN208286098U (en) * | 2018-06-04 | 2018-12-28 | 四川农业大学 | Explant sterilizing device |
CN208821412U (en) * | 2018-09-18 | 2019-05-07 | 四川农业大学 | A kind of corn tissue's culture seed disinfection device |
CN110437977A (en) * | 2019-08-10 | 2019-11-12 | 河南绽豫科技有限公司 | A kind of universal probiotic's culture device of adjustable-angle |
Family Cites Families (1)
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US20160278588A1 (en) * | 2015-03-24 | 2016-09-29 | James W. Caldwell | Bathing aid travel and drying device |
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CN202197645U (en) * | 2011-09-06 | 2012-04-25 | 广西亚热带作物研究所试验站 | Multifunctional inoculation scoop for plant tissue culture |
CN202285519U (en) * | 2011-09-30 | 2012-07-04 | 中国农业科学院棉花研究所 | Plant small seed sterilization device |
CN104026013A (en) * | 2014-06-17 | 2014-09-10 | 西北农林科技大学 | Sterile disinfection device for surfaces of plant tissues |
CN104904604A (en) * | 2015-07-10 | 2015-09-16 | 四川农业大学 | Disinfection and dewatering device for tissue culture of explants |
CN106857242A (en) * | 2015-12-11 | 2017-06-20 | 天津市华泰兰园种养殖专业合作社 | A kind of sterilization dehydration device of tissue culture explant |
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CN110437977A (en) * | 2019-08-10 | 2019-11-12 | 河南绽豫科技有限公司 | A kind of universal probiotic's culture device of adjustable-angle |
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Application publication date: 20200605 Assignee: Nanning Youguo Biotechnology Co.,Ltd. Assignor: GUANGXI SUBTROPICAL CROPS Research Institute (GUANGXI SUBTROPICAL AGRICULTURAL PRODUCTS PROCESSING RESEARCH INSTITUTE) Contract record no.: X2023980046016 Denomination of invention: A sterilization device for floating samples Granted publication date: 20221230 License type: Common License Record date: 20231108 |
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