CN206547542U - A kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch - Google Patents
A kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch Download PDFInfo
- Publication number
- CN206547542U CN206547542U CN201720077928.9U CN201720077928U CN206547542U CN 206547542 U CN206547542 U CN 206547542U CN 201720077928 U CN201720077928 U CN 201720077928U CN 206547542 U CN206547542 U CN 206547542U
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- CN
- China
- Prior art keywords
- sterilizing
- sterilization cylinder
- fixed mount
- cylinder
- folder
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch, belong to plant tissue culture technique.The device includes sterilization cylinder, fixed mount, magnetic stir bar, sterilizing folder, filter screen, leakage fluid dram and upper lid, wherein, it is upper to cover the upper end for being fastened on sterilization cylinder, fixed mount is placed in sterilization cylinder with magnetic stir bar, sterilizing folder is fixed on fixed mount by knot that can be undone by a pull, it is provided with the bottom of sterilizing bottle on leakage fluid dram, leakage fluid dram and a controlled valve is installed.By setting sterilizing bottle and sterilizing fixed mount and multiple sterilizings to press from both sides, different explants can be fixed by filter screen and steel bar on sterilizing folder, carry out bulk sterile, during sterilizing, sterilization cylinder is placed on use for laboratory magnetic stirring apparatus, start motor, the rotation of built-in stirrer forms vortex, drive sterilized solution and explant in sterilization cylinder, sterilized solution is fully contacted with vegetable material, without rocking sterilization tank manually, measured so as to reduce person works during experiment, operating efficiency is improved, and reduces harmfulness sterilized solution and human contact.
Description
Technical field
The utility model is related to a kind of Plant Tissue Breeding bactericidal unit, specifically, is related to a kind of plant explant
Semi-automatic bactericidal unit in batches.Belong to the field of plant tissue culture technique in agricultural field.
Background technology
In Plant Tissue Breeding, it is routine operation that surface sterilizing processing is carried out to plant tissue explant.It is typically to adopt
With sterilized solution immersion sterilization method, i.e., inserting explant needs repeatedly in sterilization container, sterilization process by sterilized solution or sterile
Water carries out sterilizing vibration to explant and washing is poured out.There is following defect in traditional sterilization method：（1）, conventional sterilization methods
Process is complicated, because explant such as seed, blade, stem section are of different sizes, when changing and cleaning remaining sterilized solution, smaller explant
Easily pour out and drop with liquid, cause experiment material to waste.Operating personnel are easy when being gripped with aseptic nipper or stopping explant
Cause secondary pollution and mechanical damage, experimental period extension, pollution rate rise efficiency reduction.（2）, in order that bactericidal agent in container
The effect for reaching complete sterilizing is fully contacted with explant, vibration sterilization container certain time by hand is needed, adds workload, drop
Low efficiency, extension operating personnel and the time of contact of poisonous bactericidal agent.Carry out vibration sterilization container by hand and repeatedly topple over to go out
During bacterium solution, some volatile and toxic liquids（Such as sodium hypochlorite, mercuric chloride）Work is easily revealed or directly contacts in process
Make personnel, there is potential safety hazard.（3）Different explants can not simultaneously be sterilized in same experiment, multiple sterilizings need to be prepared and held
Device is sterilized separately, and increases workload.（4）Due to can not fully remove explant surface moisture in time, vegetable material is easily caused dirty
Dye and browning.
Utility model patent on Explant surface sterilizing device now（CN2041191321U;CN203814333U;
CN203043041U;CN203040347U;CN202232459U;CN102018969B;CN201453700U;
CN204579400U etc.）, existing needs operating personnel to carry out manual handle（Such as CN20488280U）, or motor device need to be added, tie
Structure is complicated（Such as CN204579400U）, and the various present treatment of high-volume can not be carried out（Such as CN204762668U）Etc. defect.
The content of the invention
For prior art drawbacks described above, the purpose of this utility model be provide one kind semi-automatic can handle, structure compared with
Simply, the sterilizing of tissue cultures explant and dehydration device that high-volume multisample is operated are facilitated.
To achieve the above object, the utility model provides following technical scheme：
A kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch, including sterilization cylinder, fixed mount, magnetic agitation
Son, sterilizing folder, filter screen, leakage fluid dram and upper lid, upper lid are fastened on the upper end of sterilization cylinder, and fixed mount is placed in sterilizing with magnetic stir bar
In cylinder, sterilizing folder is fixed on fixed mount by knot that can be undone by a pull, is provided with the bottom of sterilization cylinder close at bottom surface on leakage fluid dram, leakage fluid dram
One controlled valve is installed.
It is used as the further scheme of the utility model：There is knot that can be undone by a pull at sterilizing folder two ends, can be fixed on fixed mount；Sterilizing is folded with
Carry the fixed strip of filter screen, can fixing different types explant.
It is used as the further scheme of the utility model：Fixed mount is cylinder type, and size is that can be put into sterilization cylinder, upper and lower ends
Shelf is pressed from both sides provided with multiple respective sockets with fixed sterilizing.
It is used as the further scheme of the utility model：Stirrer built in sterilization cylinder, it is normal that the bore of sterilization cylinder should be less than laboratory
Use magnetic stirring apparatus.The rotation of built-in stirrer forms vortex under electromagnetic force, drives sterilized solution and explant in sterilization cylinder
Body, makes sterilized solution fully be contacted with vegetable material.
It is used as the further scheme of the utility model：Sterilization cylinder bottom sets filter screen, and the bottom of sterilization cylinder is provided with close at bottom surface
One controlled valve is installed, can be drained sterilized solution by controlled valve on leakage fluid dram, leakage fluid dram.
It is used as the further scheme of the utility model：The top of sterilization cylinder is provided with openable closure, and big I is put into peace
The fixed mount of explant is filled, can also be pressed from both sides at the end of sterilizing by upper lid according to the sterilizing that sterilizing duration takes out fixed explant respectively.
Compared with the prior art, the beneficial effects of the utility model are：(1), can long agitation, without rocking and going out manually
Bacterium tank, so as to reduce the workload of experimenter, improves operating efficiency, reduces sterilized solution and human contact.(2) after, sterilizing i.e.
Can be drained sterilized solution by controlled valve, also aseptically can take out fixed explant in batches according to sterilizing duration by upper lid
Sterilizing folder, without worry explant material loss.(3) different explant sterilization times, can be controlled respectively.(4), this practicality
New structure is simple, easy to operate, can handle different types of explant simultaneously in batches, easy to utilize.
Brief description of the drawings
The utility model is further illustrated with reference to the accompanying drawings and examples.
Fig. 1 is the utility model structural representation.
Fig. 2 is the metal clip profile for fixing plant sample.
Fig. 3 is schematic flow diagram under the utility model use state.
In Fig. 1：1. covered on the magnetic stir bar 4. of 2. fixed mount of the sterilization cylinder 3. sterilizing folder leakage fluid dram 7. of 5. filter screen 6..
In Fig. 2：The fixed strip of clip main body 10. 8. knot that can be undone by a pull 9. sterilizes.
Embodiment
In Fig. 1, the top of sterilization cylinder 1 is provided with openable closure 7, and big I is put into fixed mount 2, at the end of sterilizing
Also the sterilizing folder 4 of fixed explant can be taken out respectively according to sterilizing duration by upper lid 7.Fixed mount 2 is cylinder type, and size is to put
Enter sterilization cylinder 1, the shelf of upper and lower ends presss from both sides 4 provided with multiple respective sockets with fixed sterilizing.Magnetic stir bar 3 built in sterilization cylinder,
The big I of sterilization cylinder is placed on use for laboratory magnetic stirring apparatus.The rotation of built-in stirrer forms vortex under electromagnetic force,
Sterilized solution and explant in sterilization cylinder are driven, sterilized solution is fully contacted with vegetable material.Sterilizing folder is fixed on by knot that can be undone by a pull 8
Determine on frame, filter screen 5 is set in sterilization cylinder bottom, the bottom of sterilization cylinder, which is provided with close at bottom surface on leakage fluid dram 6, leakage fluid dram, is provided with one
Controlled valve, can be drained sterilized solution by controlled valve.
In fig. 2, there is knot that can be undone by a pull 8 at 4 two ends of sterilizing folder, can be fixed on fixed mount 2；Sterilizing folder carries the fixed strip of filter screen
10, different types of explant can be fixed on 9 on sterilizing clip main body.
Use flow such as Fig. 3 of the present utility model：A. plant sample is put into the filter screen that sterilizing folder 4 is fixed first（As planted
Son）Or a ' is directly anchored to by fixed strip 10 in the main body 9 of sterilizing, such as branch, spike of rice；B. by the sterilizing with plant sample
Folder 4 is fixed on fixed mount 2 by knot that can be undone by a pull 8 again；C. the fixed mount 2 with sterilizing folder will be assembled and sterilization cylinder 1 is put into by upper lid 7
In, add sterilized solution and cover tightly lid；D. the sterilization cylinder of central in-house magnetic stir bar 3 is placed in use for laboratory magnetic stirring apparatus
On, motor is started, the rotation of built-in stirrer forms vortex under electromagnetic force, drives sterilized solution and explant in sterilization cylinder
Body, makes sterilized solution fully be contacted with vegetable material；When e. changing sterilized solution or sterilized water every time, whole sterilization cylinder is put into ultra-clean
Cover, drained sterilized liquid close to the leakage fluid dram 6 at bottom surface by the bottom of sterilization cylinder in workbench, opening, and in aseptic condition
Sterilized solution or sterilized water are added by upper lid 7 down；F. at the end of sterilizing overall process, after surplus liquid is drained only, by sterile
Operation is taken out fixed mount by upper lid；G. sterilizing folder is split on fixed mount, regard sterilizing folder as an entirety or sample
By sterilizing folder untie after blotted on aseptic filter paper or sterile-cloth after can carry out following inoculation and operate；F ' in sterilization process,
Also subsequent operation can be carried out according to single sterilizing folder is taken out by upper lid；The plant sample inadvertently fallen off in f " sterilization process can
Retained by the filter screen 5 of sterilization cylinder bottom, can at the end of sterilizing by sterile working by it is upper lid take out.During, this implementation
All parts of example had carried out autoclave sterilization processing in advance；2. step e, f, f ', f ", g lead on superclean bench
Cross sterile working progress.
Claims (5)
1. a kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch, including sterilization cylinder（1）, fixed mount（2）, magnetic force stirs
Mix son（3）, sterilizing folder（4）, filter screen（5）, leakage fluid dram（6）, upper lid（7）, it is characterised in that：Upper lid（7）It is fastened on sterilization cylinder（1）
Upper end, fixed mount（2）With magnetic stir bar（3）It is placed in sterilization cylinder（1）In, the sterilizing folder of fixed explant（4）Pass through knot that can be undone by a pull
It is fixed on fixed mount（2）On, in sterilization cylinder（1）Bottom close at bottom surface be provided with leakage fluid dram（6）, leakage fluid dram（6）On be provided with
One controlled valve.
2. bactericidal unit according to claim 1, it is characterised in that：Upper lid（7）It is fastened on sterilization cylinder（1）Upper end, under
Portion is provided with filter screen close at bottom surface（5）And leakage fluid dram（6）, leakage fluid dram（6）On a controlled valve, sterilization cylinder are installed（1）Top
Covered in portion（7）Provided with openable closure.
3. bactericidal unit according to claim 1, it is characterised in that：Sterilization cylinder（1）Built-in magnetic stir bar（3）, sterilizing
Cylinder（1）Bore should be less than placing sterilization cylinder（1）Magnetic stirring apparatus bore.
4. bactericidal unit according to claim 1, it is characterised in that：Fixed mount（2）For cylinder type, size is gone out for that can be put into
Bacterium cylinder（1）, the shelf of upper and lower ends is sterilized provided with multiple respective sockets with fixation to be pressed from both sides（4）.
5. bactericidal unit according to claim 1, it is characterised in that：Sterilizing folder（4）There is knot that can be undone by a pull at two ends（8）, with fixed mount
（2）It is flexibly connected, sterilizing folder（4）With filter screen（5）There is fixed strip（10）Connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720077928.9U CN206547542U (en) | 2017-01-22 | 2017-01-22 | A kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720077928.9U CN206547542U (en) | 2017-01-22 | 2017-01-22 | A kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206547542U true CN206547542U (en) | 2017-10-13 |
Family
ID=60358453
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720077928.9U Expired - Fee Related CN206547542U (en) | 2017-01-22 | 2017-01-22 | A kind of semi-automatic bactericidal unit of Plant Tissue Breeding explant batch |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206547542U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111226795A (en) * | 2020-03-06 | 2020-06-05 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
-
2017
- 2017-01-22 CN CN201720077928.9U patent/CN206547542U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111226795A (en) * | 2020-03-06 | 2020-06-05 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
| CN111226795B (en) * | 2020-03-06 | 2022-12-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171013 Termination date: 20190122 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |