CN216571831U - Plant tissue fully-immersed liquid treatment device - Google Patents

Abstract

The utility model provides a plant tissue full-immersion type liquid treatment device which comprises a treatment cylinder, an upper cover, a first filter screen, a second filter screen and a liquid discharge port, wherein the treatment cylinder is open, the upper cover is buckled on the upper opening of the cylinder, the first filter screen and the second filter screen are sequentially embedded in the treatment cylinder from top to bottom, and the liquid discharge port is arranged at the lower part of treatment. The beneficial effects of the utility model are: 1. a vacuum-pumping device is added at the upper part, so that the liquid is easier to permeate through the pressure reduction operation, and the liquid is easier to discharge through the pressure increase operation; 2. the complex structure of the original fixing clamp reinforcing fixed cylinder is simplified, and the first filter screen is replaced, so that a mold is manufactured, the cleaning after operation and use is simpler and more convenient, and the popularization and the application are convenient.

Description

Plant tissue fully-immersed liquid treatment device

Technical Field

The utility model relates to a plant tissue full-immersion type liquid treatment device, belonging to the plant biotechnology in the field of agriculture and forestry.

Background

In plant biotechnology, it is often necessary to react the plant with the treatment liquid for a sufficient period of time in contact therewith and then to remove the treatment liquid as cleanly as possible in order to reduce the growth effect of residual substances on the plant tissue. This operation is required for the following cases: (1) in the tissue culture, the plant is sterilized and disinfected by disinfectants such as alcohol, mercuric chloride, sodium hypochlorite and the like; (2) carrying out transgenosis or doubling treatment on the plant tissue culture, wherein agrobacterium liquid or colchicine and other biochemical substances are required; (3) in cryopreservation, plant tissues to be cryopreserved are loaded and dehydrated with vitrification protection agents. In these operations, direct liquid immersion is usually used, i.e., the plant tissue is placed in a container, directly contacted with the liquid, and after a certain period of treatment, the liquid is manually poured out. This mode of operation has the following drawbacks: (1) when the residual liquid is replaced and cleaned, the small plant tissues are easy to fall off along with the liquid, so that the experimental material is wasted. Operators are easy to cause secondary pollution and mechanical damage when clamping or blocking explants by using sterile tweezers, the experimental time is prolonged, the pollution rate is increased, and the efficiency is reduced; (2) the container is manually oscillated and the liquid needs a certain time when poured, so that the workload is increased, the efficiency is reduced, and the liquid containing toxic substances, such as mercury bichloride or colchicine, is easy to leak or directly contact with operators in the oscillating or removing process, so that potential safety hazards exist; (3) because some plant tissues are loose and porous, the surfaces of the plant tissues are rough or have fine hairs, and the internal air bubbles and the surface tension ensure that liquid is not easy to enter the tissues or gaps and can not fully contact the plant tissues, the treatment effect is influenced; (4) because the liquid on the surface of the plant tissue cannot be removed timely and fully, the plant material is easy to be polluted and browned.

Related patents mainly focus on explant surface disinfection devices (CN 204191321U; CN 203814333U; CN 203043041U; CN 203040347U; CN 202232459U; CN 102018969B; CN 201453700U; CN204579400U and the like), and have the defects that an operator needs to carry out manual treatment (such as CN20488280U) or needs to additionally arrange motor equipment, the structure is complex (such as CN204579400U), large-batch multi-sample treatment (such as CN204762668U) cannot be carried out, and the like.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned defects of the prior art, the present invention aims to provide a plant tissue fully-immersed liquid treatment device which can perform semi-automatic treatment, has a simple structure, can perform full treatment, and is convenient to operate.

The utility model is realized by the following technical scheme:

the utility model provides a plant tissue full-immersion liquid treatment device which comprises a treatment cylinder, an upper cover, a first filter screen, a second filter screen and a liquid discharge port, wherein the treatment cylinder is open, the upper cover is buckled on the upper opening of the treatment cylinder, the first filter screen and the second filter screen are sequentially embedded in the treatment cylinder from top to bottom, and the liquid discharge port is arranged at the lower part of the treatment cylinder.

Preferably, the first filter screen is funnel-shaped.

Preferably, the second screen is a plane.

Preferably, the top of the upper cover is provided with a vacuum pumping hole.

Preferably, the bottom of the treatment cylinder is also provided with a magnetic stirrer. The treatment cylinder is sized to fit on a magnetic stirrer used in a laboratory. The rotation of the built-in stirrer forms vortex under the action of electromagnetic force, and drives the treatment solution in the filter screen and the explant to fully contact the treatment solution with the plant material.

Compared with the prior art, the utility model has the following beneficial effects:

1. arranging a composite structure of a first filter screen, a disinfection cylinder and a lower stirrer, and placing a plant tissue sample to be treated in the first filter screen when in use; during treatment, the first filter screen filled with plant tissues is placed in the treatment cylinder, is immersed in treatment liquid and is placed on a magnetic stirrer for a laboratory, a motor is started, the rotation of a built-in stirrer forms vortex to drive the treatment liquid and the plant tissues in the treatment cylinder and the first filter screen, so that the materials can be conveniently treated in batches, the operation is simplified, and the contact of toxic liquid with workers is reduced;

2. a vacuumizing device is added at the upper part, vacuumizing, pressure reducing and infiltration assisting treatment is carried out through a vacuum pumping hole of the upper cover, so that the treatment liquid is fully contacted with the plant material, the liquid can be discharged through a faucet after the treatment, or the liquid is accelerated to be discharged through air inlet and pressurization of the vacuum pumping hole, the workload of experimenters and the contact of toxic liquid with personnel are reduced, and the working efficiency is improved;

3. the complex structure of the original fixing clamp reinforcing fixed cylinder is simplified, and the first filter screen is replaced, so that the die is manufactured, the operation and the cleaning after the use are simpler and more convenient, and the popularization and the application are convenient.

Drawings

Other features, objects and advantages of the utility model will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

FIG. 1 is a schematic structural view of the present invention;

in the figure: 1. a treatment cylinder; 2. a first filter screen; 3. a magnetic stirrer 4, a vacuum pumping hole 5 and a second filter screen; 6. a liquid discharge port; 7. and (7) covering.

Detailed Description

The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the utility model, but are not intended to limit the utility model in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the utility model. All falling within the scope of the present invention.

The utility model provides a plant tissue full-immersion liquid treatment device, which is structurally shown in figure 1 and comprises a treatment cylinder 1, an upper cover 7, a first filter screen 2, a second filter screen 5 and a liquid discharge port 6, wherein the treatment cylinder 1 is open, the upper cover 7 is buckled at the upper opening of the treatment cylinder 1, the first filter screen 2 and the second filter screen 5 are sequentially embedded in the treatment cylinder 1 from top to bottom, and the liquid discharge port 6 is arranged at the lower part of the treatment cylinder.

The first filter screen 2 is funnel-shaped.

The second filter 5 is planar.

The top of the upper cover 7 is provided with a vacuum pumping hole 4.

The bottom of the treatment cylinder 1 is also provided with a magnetic stirrer 3. The treatment cylinder is sized to fit on a magnetic stirrer used in a laboratory. The rotation of the built-in stirrer forms vortex under the action of electromagnetic force, and drives the treatment solution in the filter screen and the explant to fully contact the treatment solution with the plant material.

Specifically, the top of the treatment cylinder 1 is provided with an openable sealing cover 7, the funnel-shaped first filter screen 2 can be placed in the size of the sealing cover, and explants can be respectively taken out by the upper cover 7 according to the toxic time when the treatment is finished. Handle 1 built-in magnetic stirrers 3 in a section of thick bamboo, on second filter screen 5 can be arranged in to first filter screen 2, be magnetic stirrers 3 down, whole device can arrange the laboratory in during the processing on magnetic stirrers, and the rotation of built-in stirrers forms the vortex under the electromagnetic force effect, drives and handles interior processing liquid and plant material of a section of thick bamboo, makes the processing liquid fully contact with plant material. The lower part of the treatment cylinder is provided with a second filter screen 5, the lower part of the treatment cylinder is provided with a liquid outlet 6 close to the bottom surface, a water tap can be arranged on the liquid outlet, liquid can be discharged through the water tap, the upper cover 7 is provided with a vacuum pumping port 4, the vacuum pumping can be carried out when the liquid submerges tissues to reduce the pressure so that the liquid can easily permeate the tissues, and the air can be admitted through the pumping port (4) to carry out pressurization operation when the liquid is discharged so as to accelerate the discharge of the liquid.

The application process of the utility model comprises the following steps:

a. firstly, putting a plant sample into a first filter screen 2;

b. putting the first filter screen 2 with the plant sample into the treatment cylinder 1 through the upper cover 7, adding treatment liquid and tightly covering the upper cover;

c. placing a treatment cylinder with a magnetic stirrer 3 arranged in the center on a magnetic stirrer for a laboratory, starting a motor, forming vortex by the rotation of the built-in stirrer under the action of electromagnetic force, and driving treatment liquid and plant tissues in the treatment cylinder to fully contact the liquid and the plant tissues;

d. the vacuum air pump is connected with the vacuum air pumping port 4 on the upper cover 7 to perform air pumping, pressure reduction and infiltration assisting treatment;

e. when the liquid is replaced each time, the whole treatment cylinder is placed in a super-clean workbench, the upper cover is opened, the treatment liquid is discharged through a liquid outlet 6 at the lower part of the treatment cylinder close to the bottom surface, and the treatment liquid or the sterile water is added through the upper cover 7 under the aseptic condition;

f. when the whole treatment process is finished, after the redundant liquid is drained, the first filter screen 2 is taken out through the upper cover 7 through aseptic operation;

g. the plant tissue is removed from the first screen 2 and may be subsequently processed after blotting on sterile filter paper or cloth.

(Note: 1. all parts have been previously autoclaved; 2. Steps a, e., f, g are performed by aseptic manipulation on a clean bench.

The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the utility model.

Claims (5)

1. The utility model provides a plant tissue soaks formula liquid treatment device entirely, its characterized in that, is including handling a section of thick bamboo, upper cover, first filter screen, second filter screen and leakage fluid dram, it is uncovered form to handle a section of thick bamboo, the upper cover lock is in the last mouth of handling a section of thick bamboo, first filter screen and second filter screen from top to bottom inlay in proper order and establish in handling a section of thick bamboo, the leakage fluid dram sets up in the lower part of handling a section of thick bamboo.

2. The plant tissue flooded-type liquid treatment apparatus of claim 1, wherein the first screen is funnel-shaped.

3. The plant tissue flooded-type liquid treatment apparatus as claimed in claim 1, wherein the second screen is planar.

4. The plant tissue total immersion liquid processing apparatus as claimed in claim 1, wherein a vacuum suction port is provided on a top portion of said upper cover.

5. The apparatus according to claim 1, wherein a magnetic stirrer is further provided at the bottom of the treatment tank.

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