CN104026013A - Sterile disinfection device for surfaces of plant tissues - Google Patents
Sterile disinfection device for surfaces of plant tissues Download PDFInfo
- Publication number
- CN104026013A CN104026013A CN201410269159.3A CN201410269159A CN104026013A CN 104026013 A CN104026013 A CN 104026013A CN 201410269159 A CN201410269159 A CN 201410269159A CN 104026013 A CN104026013 A CN 104026013A
- Authority
- CN
- China
- Prior art keywords
- sterilizer
- fungi
- axle
- blade
- hinged
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004659 sterilization and disinfection Methods 0.000 title abstract description 17
- 230000006835 compression Effects 0.000 claims abstract description 14
- 238000007906 compression Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 238000005660 chlorination reaction Methods 0.000 claims description 8
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 8
- 229940033663 thimerosal Drugs 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract 3
- 241000894006 Bacteria Species 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000000739 chaotic effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a sterile disinfection device for surfaces of plant tissues. According to the device, disinfector shafts and a plurality of independent disinfectors are respectively arranged among groove-shaped vertical plates and are hinged with anti-bacterial plate hinging shafts and anti-bacterial plates; sample grooves are formed in the bottom surfaces of the vertical plates and are communicated with liquid injecting holes; fixed compression bars and cutting blades which can move up and down are arranged in the disinfectors; the sample grooves corresponding to the lower parts of the disinfectors are respectively shielded by the independent anti-bacterial plates and are mutually isolated, so that plant samples can be simultaneously fixed in batches, i.e., the unified comprehensive sterilization can be carried out. Thus, the operation processes are reduced; the time is saved; the disinfection effects on the surfaces of the plant samples are improved; and the plant samples can be accurately numbered, so that the disordered sequence is avoided. The small batch treatment and the isolated bacteria control for the plant samples are carried out in the independent disinfectors, so that the secondary pollution caused by long operation time and complicated operations in subsequent separation and culture operations is effectively avoided. As a result, various defects in the existing traditional disinfection effect are solved.
Description
Technical field
The present invention relates to a kind of aseptic chlorination equipment, a kind of plant tissue surface sterile chlorination equipment specifically, be applicable to the plant tissue surface sterile sterilization before the separated cultivation of microorganism in the Plant Tissue Breeding such as microbiology, forest, agronomy, plant tissue, belong to biological technical field.
Background technology
In the separated incubation of Plant Tissue Breeding and microorganism in plant tissue, in order to obtain desirable pure culture, must carry out surface sterilization thoroughly to plant tissue sample processes, the eternal loss of activity of microorganism that its surface is carried, and in follow-up operation, avoid the secondary pollution of plant sample, this is directly to have influence on the separated key of cultivating success or not of microorganism in Plant Tissue Breeding and plant tissue.
Traditional plant tissue surface sterile sterilization method is to adopt the single mode of processing respectively, with tweezers one by one gripping plant sample put into medicining liquid dipping sterilizing, then use sterile water washes clean.Whole operating process is too loaded down with trivial details and consuming time, cause operating efficiency degradation, when especially plant sample is more, gripping plant sample is implemented a large amount of single repetitive operation frequently, inefficiency not only, sterilization effect is unstable, also easily causes sample number chaotic, or cause the serious consequence of secondary pollution, directly affect the success rate of the separated cultivation of microorganism in Plant Tissue Breeding and plant tissue.Still do not have at present a kind of aseptic chlorination equipment can improve the efficiency of plant tissue surface sterile sterilization, can avoid preferably with serious pollution problem again.
Summary of the invention
The object of the invention is to solve the loaded down with trivial details and unsettled problem of sterilization effect of current traditional plant tissue surface aseptic sterilisation methods complicated operation, and a kind of plant tissue surface sterile chlorination equipment is provided.This installs not only a large amount of plant sample to be sterilized of batch process quickly and easily, can also effectively avoid the secondary pollution in operating process, improves sterilization effect.
The technical scheme that realizes the object of the invention is as follows:
On the vertical facade at grooved bottom board two ends, be respectively equipped with sterilizer track, sterilizer axle is hinged between the sterilizer track at two ends, sterilizer axle two ends are respectively provided with adjusting nut, several sterilizers are connected on sterilizer axle, fungi-proofing plate hinge is also hinged between the vertical facade in two ends, several fungi-proofing plates are hinged on fungi-proofing plate hinge, position corresponding with sterilizer on grooved bottom board bottom surface is respectively equipped with sample slot, in it, be marked with thimerosal, liquid injection hole is positioned at the position of grooved bottom board edge and close sample slot, between each sample slot and between sample slot and liquid injection hole, mutually connect, in sterilizer, be provided with compression column with fixed movable up and down and cutting blade, cutting blade cover is connected on the blade sliding axle that the right and left is provided with blade spring, by spring tension, cutting blade is expressed to the upper end of blade sliding axle, the top of cutting blade extends to sterilizer and connects blade handle outward, compression column with fixed cover is connected on the depression bar sliding axle that the right and left is provided with Pressure-bar spring, by spring tension, compression column with fixed is expressed to the lower end of depression bar sliding axle, sample slot corresponding to each sterilizer and bottom all has independent fungi-proofing plate to cover, and isolate with adjacent overlay area.
The present invention can mass simultaneous fixing plant sample, the comprehensive sterilizing of unifying, has not only improved the effect of plant sample surface sterilization, has saved a large amount of time, can also unify mark to plant sample, accurately numbering, avoids order chaotic.In this external independently sterilizer, plant sample is carried out a small amount of batch processing and isolates fungi-proofing, the secondary pollution phenomenon that can also effectively avoid long due to the operating time in follow-up separation and cultivation operate, frequent operation causes, has solved the various drawbacks that exist in current traditional sterilizing methods effectively.
Accompanying drawing explanation
Accompanying drawing 1 is that the master of the embodiment of the present invention looks part section structural representation.
Accompanying drawing 2 is plan structure schematic diagrames of the embodiment of the present invention.
Accompanying drawing 3 is right TV structure schematic diagrames of the embodiment of the present invention.
Accompanying drawing 4 are embodiment of the present invention accompanying drawings 3 along A-A to generalized section.
Accompanying drawing 5 are embodiment of the present invention accompanying drawings 3 along B-B to generalized section.
Description of reference numerals is as follows:
Grooved bottom board 1, sterilizer 2, sterilizer track 3, fungi-proofing plate 4, fungi-proofing plate hinge 5, sterilizer axle 6, adjusting nut 7, compression column with fixed 8, depression bar sliding axle 9, Pressure-bar spring 10, cutting blade 11, blade handle 12, blade sliding axle 13, blade spring 14, liquid injection hole 15, sample slot 16, thimerosal 17, plant sample 18.
Embodiment
Below in conjunction with embodiment and accompanying drawing, technical scheme of the present invention is described in further detail:
On the vertical facade at grooved bottom board (1) two ends, be respectively equipped with sterilizer track (3), sterilizer axle (6) is hinged between the sterilizer track (3) at two ends, sterilizer axle (6) two ends are respectively provided with adjusting nut (7), three sterilizers (2) are connected on sterilizer axle (6), fungi-proofing plate hinge (5) is also hinged between the vertical facade in two ends, upper three the fungi-proofing plates (4) that are hinged of fungi-proofing plate hinge (5), position corresponding with sterilizer (2) on grooved bottom board (1) bottom surface is respectively equipped with sample slot (16), in it, be marked with thimerosal (17), liquid injection hole (15) is positioned at the position of grooved bottom board (1) edge and close sample slot (16), between each sample slot (16) and between sample slot (16) and liquid injection hole (15), mutually connect, in sterilizer (2), be provided with compression column with fixed movable up and down (8) and cutting blade (11), cutting blade (11) cover is connected on the blade sliding axle (13) that the right and left is provided with blade spring (14), by spring tension, cutting blade (11) is expressed to the upper end of blade sliding axle (13), the top of cutting blade (11) extends to the outer blade handle (12) that connects of sterilizer (2), compression column with fixed (8) cover is connected on the depression bar sliding axle (9) that the right and left is provided with Pressure-bar spring (10), by spring tension, compression column with fixed (8) is expressed to the lower end of depression bar sliding axle (9), the bottom of compression column with fixed (8) is dentation, sample slot (16) corresponding to each sterilizer (2) and bottom all has independent fungi-proofing plate (4) to cover, and isolate with adjacent overlay area.
Before the present invention uses, adopt autoclaving to carry out thorough sterilizing to this device, follow-up all operations all carries out in aseptic superclean bench.First unscrew adjusting nut and by sterilizer horizontal slip to away from one end of sample slot, with tweezers, lightly plant tissue sample is placed in sample slot in order one by one, according to the size of plant sample, determine the sample size of placing in each sample slot.Subsequently sterilizer is moved to vertical rail down sliding, plant sample is gripped in grooved bottom board and fixedly between compressing tablet, after compression, screw adjusting nut, prevent the loosening plant sample loss that causes, and cover fungi-proofing plate, mark sample number on fungi-proofing plate, then draw a certain amount of thimerosal and injected by liquid injection hole, guaranteeing has enough thimerosals, the completely all plant samples of submergence in each sample slot.Sterilization time is determined according to the difference of plant sample and sterilisation purpose, as the tip of a root surface sterilization of separated Applying Ectomycorrhizal Fungi from the tip of a root, the tip of a root need be soaked in thimerosal to 3-4 minute, finally rapidly by thimerosal by liquid injection hole sucking-off, and use the same method and inject sterile water by plant sample repeated washing 3-4 time, to remove the thimerosal of its remained on surface.After having sterilized, as far as possible by the whole sucking-offs of cleaning solution, and raise one by one fungi-proofing plate, depress cutting blade plant sample is cut off, with tweezers one by one gripping plant sample carry out follow-up separation and cultivate operation.After EO, this device is cleaned up in order to reusing, later all must sterilizing before each use.
Claims (3)
1. a plant tissue surface sterile chlorination equipment, it is characterized in that technical scheme of the present invention is as follows: on the vertical facade at grooved bottom board (1) two ends, be respectively equipped with sterilizer track (3), sterilizer axle (6) is hinged between the sterilizer track (3) at two ends, sterilizer axle (6) two ends are respectively provided with adjusting nut (7), several sterilizers (2) are connected on sterilizer axle (6), fungi-proofing plate hinge (5) is also hinged between the vertical facade in two ends, upper several the fungi-proofing plates (4) that are hinged of fungi-proofing plate hinge (5), position corresponding with sterilizer (2) on grooved bottom board (1) bottom surface is respectively equipped with sample slot (16), in it, be marked with thimerosal (17), liquid injection hole (15) is positioned at the position of grooved bottom board (1) edge and close sample slot (16), between each sample slot (16) and between sample slot (16) and liquid injection hole (15), mutually connect, in sterilizer (2), be provided with compression column with fixed movable up and down (8) and cutting blade (11), cutting blade (11) cover is connected on the blade sliding axle (13) that the right and left is provided with blade spring (14), by spring tension, cutting blade (11) is expressed to the upper end of blade sliding axle (13), the top of cutting blade (11) extends to the outer blade handle (12) that connects of sterilizer (2), compression column with fixed (8) cover is connected on the depression bar sliding axle (9) that the right and left is provided with Pressure-bar spring (10), by spring tension, compression column with fixed (8) is expressed to the lower end of depression bar sliding axle (9), sample slot (16) corresponding to each sterilizer (2) and bottom all has independent fungi-proofing plate (4) to cover, and isolate with adjacent overlay area.
2. a kind of plant tissue surface sterile chlorination equipment according to claim 1, is characterized in that: three sterilizers (2) that are connected on sterilizer axle (6), upper three the fungi-proofing plates (4) that are hinged of fungi-proofing plate hinge (5).
3. a kind of plant tissue surface sterile chlorination equipment according to claim 1, is characterized in that: the bottom of compression column with fixed (8) is dentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410269159.3A CN104026013B (en) | 2014-06-17 | 2014-06-17 | Sterile disinfection device for surfaces of plant tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410269159.3A CN104026013B (en) | 2014-06-17 | 2014-06-17 | Sterile disinfection device for surfaces of plant tissues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104026013A true CN104026013A (en) | 2014-09-10 |
| CN104026013B CN104026013B (en) | 2015-07-08 |
Family
ID=51457181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410269159.3A Expired - Fee Related CN104026013B (en) | 2014-06-17 | 2014-06-17 | Sterile disinfection device for surfaces of plant tissues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104026013B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111226795A (en) * | 2020-03-06 | 2020-06-05 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
| CN113287523A (en) * | 2021-06-08 | 2021-08-24 | 陈嘉裔 | Intermittent fast-immersion succulent plant propagation and disinfection device and implementation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01158957A (en) * | 1987-12-11 | 1989-06-22 | Komatsu Ltd | Sterilizing apparatus |
| WO2004114378A1 (en) * | 2003-06-19 | 2004-12-29 | Hokkaido Technology Licensing Office Co., Ltd. | Clean unit, clean unit system, functional unit, functional unit system, material treatment method, element manufacturing method, cell line culturing method, and plant body culturing method |
| CN201453700U (en) * | 2009-06-05 | 2010-05-12 | 重庆大学 | Explant surface sterilizing device |
| CN102018969A (en) * | 2010-11-30 | 2011-04-20 | 上海交通大学 | Device for disinfecting surfaces of explants of plants |
| CN102166367A (en) * | 2010-12-30 | 2011-08-31 | 西北农林科技大学 | Method for AMF spore surface disinfection |
| CN202459434U (en) * | 2011-12-02 | 2012-10-03 | 刘家迅 | Plant tissue explant sterilizing device |
| CN203538050U (en) * | 2013-09-16 | 2014-04-16 | 上海市农业科学院 | Cleaning sterilizer for tissue culture explant of aquatic plant |
-
2014
- 2014-06-17 CN CN201410269159.3A patent/CN104026013B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01158957A (en) * | 1987-12-11 | 1989-06-22 | Komatsu Ltd | Sterilizing apparatus |
| WO2004114378A1 (en) * | 2003-06-19 | 2004-12-29 | Hokkaido Technology Licensing Office Co., Ltd. | Clean unit, clean unit system, functional unit, functional unit system, material treatment method, element manufacturing method, cell line culturing method, and plant body culturing method |
| CN201453700U (en) * | 2009-06-05 | 2010-05-12 | 重庆大学 | Explant surface sterilizing device |
| CN102018969A (en) * | 2010-11-30 | 2011-04-20 | 上海交通大学 | Device for disinfecting surfaces of explants of plants |
| CN102166367A (en) * | 2010-12-30 | 2011-08-31 | 西北农林科技大学 | Method for AMF spore surface disinfection |
| CN202459434U (en) * | 2011-12-02 | 2012-10-03 | 刘家迅 | Plant tissue explant sterilizing device |
| CN203538050U (en) * | 2013-09-16 | 2014-04-16 | 上海市农业科学院 | Cleaning sterilizer for tissue culture explant of aquatic plant |
Non-Patent Citations (1)
| Title |
|---|
| 吴坤林等: "番木瓜外植体消毒方法研究", 《福建林业科技》, vol. 35, no. 3, 30 September 2008 (2008-09-30), pages 76 - 80 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111226795A (en) * | 2020-03-06 | 2020-06-05 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
| CN111226795B (en) * | 2020-03-06 | 2022-12-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
| CN113287523A (en) * | 2021-06-08 | 2021-08-24 | 陈嘉裔 | Intermittent fast-immersion succulent plant propagation and disinfection device and implementation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104026013B (en) | 2015-07-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104250616A (en) | Separation method for plant endophyte | |
| CN106668891A (en) | Medical appliance cleaning circulation sterilization drying device | |
| CN103667080B (en) | A kind of method of separating plant pathogenic fungi | |
| CN205649664U (en) | Scalpel degassing unit is used in medical treatment | |
| CN104026013B (en) | Sterile disinfection device for surfaces of plant tissues | |
| EP3505194A3 (en) | Process and apparatus for cleaning, disinfection, sterilization, or combinations thereof | |
| CN104819865A (en) | Sterile sampling packet and assembly sampling method thereof | |
| CN102934611B (en) | Method for collecting embryo from paeonia suffruticosa seed and for inoculation | |
| CN104862215B (en) | Microbial sample processing and scribing system and method | |
| CN204972359U (en) | Double -deck medicinal disinfection groove | |
| CN205307386U (en) | Acupuncture box with from disinfection function | |
| CN205268665U (en) | Three -dimensional disinfection cleaning device of international goods storage rack | |
| CN106171997B (en) | A kind of while explant surface and endophyte when removing Plant Tissue Breeding method | |
| CN204191321U (en) | A kind of fountain Explant sterilizing device | |
| WO2008097884A3 (en) | Processing skin from living donors | |
| CN204169371U (en) | Aseptic material storage cabinet | |
| CN201203539Y (en) | Vibration type microtome for tissue culture | |
| CN204219432U (en) | A kind of decontaminating apparatus for surgical medical small appliance | |
| CN102823446A (en) | Efficient method for sugarcane infection by smut spores | |
| CN205884283U (en) | Tissue culture seed disinfection oscillator | |
| CN106613951A (en) | Method for disinfection of explant in tissue culture | |
| CN208641342U (en) | A kind of medical automatic disinfection device | |
| CN107258539B (en) | A method of control taipei fritillary bulb tissue cultures endophytic bacterial contamination | |
| CN209802778U (en) | Disposable sterile pyrogen-free multifunctional tissue disruptor | |
| CN205250040U (en) | Tissue culture in -process seed rataria degassing unit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150708 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |