CN201203539Y - Vibration type microtome for tissue culture - Google Patents

Vibration type microtome for tissue culture Download PDF

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Publication number
CN201203539Y
CN201203539Y CNU2008200812907U CN200820081290U CN201203539Y CN 201203539 Y CN201203539 Y CN 201203539Y CN U2008200812907 U CNU2008200812907 U CN U2008200812907U CN 200820081290 U CN200820081290 U CN 200820081290U CN 201203539 Y CN201203539 Y CN 201203539Y
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CN
China
Prior art keywords
vibration
slicer
tissue culture
shaker arm
knife rest
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CNU2008200812907U
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Chinese (zh)
Inventor
兰峰
沈丽
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Individual
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Individual
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Priority to CNU2008200812907U priority Critical patent/CN201203539Y/en
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Publication of CN201203539Y publication Critical patent/CN201203539Y/en
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Expired - Fee Related legal-status Critical Current

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Abstract

A vibration type slicer applied to tissue culture relates to a medical mechanical device, especially a slicer for biomedical tissue culture, which is composed of a base, a vibration arm, a knife rack, a knife blade, a sample platform, a vibration system and a step-by-step system, the vibration arm is mounted on the base by the vibration system, the knife rack is mounted on the vibration arm, the knife blade is mounted on the knife rack, the sample platform is mounted on the step-by-step system and is positioned rightly under the vibration arm; the vibration system is composed of an electric machine and drive gears, the step-by-step system is composed of an electric control translational platform and a step-by-step electric machine controller, a damping spring is mounted between the vibration arm and the knife rack. The slicer overcomes the problem of mechanical cutting generation amplification, can be applied to amplification cultivation of tissue slice, the damping spring can enable the slicing force to be more even and can protect the knife blade, the slicer volume is small, the slicer is suitable for ultraviolet ray disinfecting and slicing in the superclean workbench.

Description

A kind of oscillatory type microtome that is applied to tissue culture
Technical field
The utility model relates to a kind of medical machinery equipment, especially a kind of microtome that is used for biomedical tissue cultivation usefulness.
Background technology
Histotomy is widely used in biological and basis and clinical medicine domain, it mainly contains two class histotomies and uses, the one, fixedly the paraffin of embedding and freezing ultra-thin section are that the slice of fresh biological tissue is used for tissue culture to carry out morphology research in addition.Tissue culture is the experimental technique that often is applied in the biological and medical field research, has cellular incubation and piece of tissue to cultivate two kinds.Wherein, the histotome that animal tissue cultivates mainly is that the brain sheet that is applied in the Neuroscience Research is cultivated, though this microtome is powerful, volume is big, complex structure, price are high, and it is designed primarily to the service of cultivating of brain sheet, so range of application is very limited.At present, the human embryo neural stem cells that is used for the treatment of multiple sacred disease only can be with the machine cuts amplification of going down to posterity, and traditional histotome is difficult to use in this technology.Therefore need to improve existing microtome technology, can be applied to the amplification cultivation of histotomy.
Summary of the invention
Technical problem to be solved in the utility model is that present microtome does not fit into and organizes amplification cultivation, and a kind of cheap oscillatory type tissue culture microtome is provided.
The utility model is made of support, shaker arm, knife rest, blade, sample stage, vibrational system and step-by-step system, shaker arm is installed on the support by vibrational system, and knife rest is installed on the shaker arm, and blade is installed on the knife rest, sample stage is installed on the step-by-step system, is positioned under the shaker arm; Vibrational system is made of motor and transmission gear, and step-by-step system is made of electronic control translation stage and controllor for step-by-step motor, it is characterized in that having installed between shaker arm and the knife rest damping spring.
Wherein cutter frame structure is to pass plastic tube by an iron wire, and iron wire one end is provided with snap ring and is stuck on the plastic tube, and the other end is connected with nut clip, the middle damping spring of installing.
Because iron wire can slide in plastic tube, sliding scale is subjected to the restriction of damping spring and the terminal snap ring of iron wire, can be because of blade distortion or slide are broken in the process of cutting.
This microtome has overcome the drawback that machine cuts goes down to posterity and increases; can be applied to the amplification cultivation of histotomy; with respect to traditional vibratome, shaker arm contains a damping spring can make the dynamics of section more even, and can play the effect of protection blade.Blade fastens with two nut clip, and platform is adjusted its tangent plane per sample.Microtome volume of the present utility model is little, is applicable in superclean bench to help guaranteeing the aseptic condition of tissue culture with disinfection by ultraviolet light and section.
Description of drawings
Fig. 1 is a structural representation of the present utility model
Wherein, support 1, shaker arm 2, knife rest 3, blade 4, sample stage 5, vibrational system 6, step-by-step system 7, damping spring 8, iron wire 13, snap ring 14, nut clip 15, plastic tube 16, motor 9, transmission gear 1G, electronic control translation stage 11, controllor for step-by-step motor 12
Embodiment
Below in conjunction with accompanying drawing the utility model is done progressive explanation:
A kind of oscillatory type microtome that is applied to tissue culture, constitute by support 1, shaker arm 2, knife rest 3, blade 4, sample stage 5, vibrational system 6 and step-by-step system 7, shaker arm 2 is installed on the support 1 by vibrational system 6, knife rest 3 is to pass plastic tube 16 by an iron wire, iron wire one end is provided with snap ring 14 and is stuck on the plastic tube 16, the other end is connected with nut clip 15, the middle damping spring 8 of installing, blade 4 is anchored on the knife rest 3 by nut clip, sample stage 5 is installed on the step-by-step system 7, is positioned under the shaker arm 2; Vibrational system 6 is made of motor 9 and transmission gear 10, and motor can drive shaker arm by transmission gear, under the voltage stabilizing direct current supply condition of 3v, is in vertical reciprocating motion with the frequency of 2.5hz.Step-by-step system 7 is made of electronic control translation stage 11 and controllor for step-by-step motor 12, and the double dish of sample is fixed on the electronic control translation stage 11, and as required speed is made level and all around moved under the control of controllor for step-by-step motor.

Claims (2)

1, a kind of oscillatory type microtome that is applied to tissue culture is made of support (1), shaker arm (2), knife rest (3), blade (4), sample stage (5), vibrational system (6) and step-by-step system (7), shaker arm (2) is installed on the support (1) by vibrational system (6), knife rest (3) is installed on the shaker arm (2), blade (4) is installed on the knife rest (3), sample stage (5) is installed on the step-by-step system (7), is positioned under the shaker arm (2); Vibrational system (6) is made of motor (9) and transmission gear (10), and step-by-step system (7) is made of electronic control translation stage (11) and controllor for step-by-step motor (12), it is characterized in that between shaker arm (2) and the knife rest (3) damping spring (8) being installed.
2, a kind of oscillatory type microtome that is applied to tissue culture according to claim 1, it is characterized in that knife rest (3) structure is to pass plastic tube (16) by an iron wire (13), iron wire (13) one ends are provided with snap ring (14) and are stuck on the plastic tube (16), the other end is connected with nut clip (15), the middle damping spring (8) of installing.
CNU2008200812907U 2008-06-02 2008-06-02 Vibration type microtome for tissue culture Expired - Fee Related CN201203539Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNU2008200812907U CN201203539Y (en) 2008-06-02 2008-06-02 Vibration type microtome for tissue culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNU2008200812907U CN201203539Y (en) 2008-06-02 2008-06-02 Vibration type microtome for tissue culture

Publications (1)

Publication Number Publication Date
CN201203539Y true CN201203539Y (en) 2009-03-04

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Family Applications (1)

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CNU2008200812907U Expired - Fee Related CN201203539Y (en) 2008-06-02 2008-06-02 Vibration type microtome for tissue culture

Country Status (1)

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CN (1) CN201203539Y (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102445359A (en) * 2011-12-20 2012-05-09 深圳市华测检测技术股份有限公司 Sample surface coating sampling equipment
CN104634609A (en) * 2015-02-06 2015-05-20 大连理工大学 Metal wire tool for minimally invasive cutting of biological soft tissues
CN108486043A (en) * 2018-02-02 2018-09-04 中南大学湘雅医院 Cartilage tissue cutting and enzymolysis digestion integrated device and cartilage tissue treatment method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102445359A (en) * 2011-12-20 2012-05-09 深圳市华测检测技术股份有限公司 Sample surface coating sampling equipment
CN104634609A (en) * 2015-02-06 2015-05-20 大连理工大学 Metal wire tool for minimally invasive cutting of biological soft tissues
CN108486043A (en) * 2018-02-02 2018-09-04 中南大学湘雅医院 Cartilage tissue cutting and enzymolysis digestion integrated device and cartilage tissue treatment method
CN108486043B (en) * 2018-02-02 2019-03-19 中南大学湘雅医院 Cartilage tissue cutting and enzymolysis digestion integrated device and cartilage tissue treatment method

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Granted publication date: 20090304

Termination date: 20110602