CN105269615B - Automatic umbilical cord Huatong glue dicer - Google Patents

Automatic umbilical cord Huatong glue dicer Download PDF

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Publication number
CN105269615B
CN105269615B CN201510521993.1A CN201510521993A CN105269615B CN 105269615 B CN105269615 B CN 105269615B CN 201510521993 A CN201510521993 A CN 201510521993A CN 105269615 B CN105269615 B CN 105269615B
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groove plate
cutting groove
longitudinal
transverse
positioning cutting
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CN105269615A (en
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蓝庆
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Avic Ningxia Biology Co ltd
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Avic Ningxia Biology Co ltd
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Abstract

An automatic dicer of umbilical cord hua tong gum, comprising: the device comprises a frame, a cutter mechanism, a transverse positioning cutting groove plate and a longitudinal positioning cutting groove plate; the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are used for clamping umbilical cord glue, the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are locked through the locking mechanism, the locking mechanism provides force for mutual clamping of the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate, a group of transverse feed holes are distributed on the transverse positioning cutting groove plate, a group of longitudinal feed holes are distributed on the longitudinal positioning cutting groove plate, the feed holes are strip-shaped through holes, the cutter mechanism is used for automatically completing feeding, cutting and retracting actions, and a row of blades which are arranged at equal intervals are arranged on the blade supporting rods of the cutter mechanism. The automatic cutting machine for the umbilical cord Huatong glue can overcome the influence of low efficiency, uneven hand cutting size and unevenness caused by artificial fatigue, and enables the cutting of the umbilical cord Huatong glue to be smooth, regular and efficient.

Description

Automatic umbilical cord Huatong glue dicer
Technical field:
the invention relates to the technical field of biological sample extraction, in particular to an automatic umbilical cord hua tong gum dicer.
The background technology is as follows:
modern cell biology research shows that stem cells with self-renewal and multipotency can be applied to the treatment and rehabilitation of various diseases. However, with the development of intensive research and clinical application, stem cells are found to have problems, for example, ethical, legal, immune rejection, tumorigenicity and the like in the application of embryonic stem cells; the autologous bone marrow mononuclear cells have the problems of complex components, low stem cell content with multidirectional differentiation potential, limited differentiation potential and the like; autologous bone marrow MSCs are limited in source, cell proliferation and differentiation potential is obviously reduced along with the increase of age, and in-vitro long-term expansion reduces in-vivo differentiation homing potential. The existing extraction means of autologous bone marrow often brings great physiological pain to patients, and the problems seriously restrict the research of stem cells, especially mesenchymal stem cells in clinical application.
In response to the above problems, in recent years, with the increasing reports of related studies, researchers have shifted the field of view of Mesenchymal Stem Cell (MSC) sources to umbilical cord blood, umbilical cord and placenta. They have the particular advantage: the source is sufficient, the probability of virus pollution is low, the immunogenicity is weak, the cells are more original, the disputes in ethical law are avoided, and the operation is simple and convenient. Obtaining umbilical cord mesenchymal stem cells is a very efficient method. Today, there are two methods for obtaining umbilical cord mesenchymal stem cells: 1. tissue block adherence method; 2. enzymatic digestion
The tissue block adhering process includes the steps of obtaining neonate umbilical cord, washing away residual umbilical cord blood with isotonic buffer salt solution or physiological saline solution, eliminating umbilical artery, umbilical vein and the outer amniotic membrane of umbilical cord in a sterile and clean biosafety cabinet, extracting to obtain Huatong glue, cutting the Huatong glue into small blocks, and subsequent inoculation onto the bottom plate of culture dish for culture to obtain cell.
The enzyme digestion method is to digest the Huatong glue by the active enzyme of single component or composite component and obtain the cell from the digestion liquid.
The two methods have advantages and disadvantages:
1. tissue block adherence method: the method has the advantages of simple operation, low cost, relatively fixed operation standard and relatively uniform cells obtained by culture. The disadvantage is the long primary culture time (7-10 days visible primary cell climbing out).
2. Enzymatic digestion: the advantage is that the procedure is rapid and the primary culture time is very short (a large number of primary cells are visible for 3 d). The disadvantages are that the cost is high, the cells obtained by culture are not uniform, the enzyme use method in the digestion process is not uniform, and the enzyme liquid can damage the cells when the digestion time is too long.
Therefore, the method for obtaining umbilical cord Mesenchymal Stem Cells (MSC) is widely used at present, and is mostly carried out by a tissue mass adherence method.
At present, the umbilical cord Huatong glue is manually cut to obtain umbilical cord Huatong glue cut blocks for storage, but the umbilical cord Huatong glue is limited by the high toughness and the smooth surface of the Huatong glue and the narrow space for operation, the efficiency of cutting the umbilical cord Huatong glue is extremely low, and the difficulty of obtaining MSC by a tissue block adherence method is great occupied manpower and time. In order to make the cutting of the umbilical cord gum quick and accurate and obtain better utilization rate or economic benefit of the umbilical cord gum, a special gum cutting tool needs to be designed.
The invention comprises the following steps:
in view of this, it is necessary to design an automatic umbilical cord gum dicer capable of efficiently cutting umbilical cord gum according to the size and shape requirements.
An automatic dicer of umbilical cord hua tong gum, comprising: the device comprises a frame, a cutter mechanism, a transverse positioning cutting groove plate and a longitudinal positioning cutting groove plate; the machine frame is used for fixedly arranging a cutter mechanism, a transverse positioning cutting groove plate and a longitudinal positioning cutting groove plate, the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are used for clamping umbilical cord glue, the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are locked through a locking mechanism, the locking mechanism provides force for mutually clamping the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate, a group of transverse feed holes are distributed on the transverse positioning cutting groove plate, a group of longitudinal feed holes are distributed on the longitudinal positioning cutting groove plate, the feed holes are strip-shaped through holes, grooves are respectively formed in clamping surfaces of the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate, the grooves of the longitudinal positioning cutting groove plate correspond to the longitudinal feed holes, the cutter mechanism is used for automatically completing feeding, cutting and retracting actions, and a row of blades which are arranged at equal intervals are arranged on a blade supporting rod of the cutter mechanism and extend/retract intention blades into/retract from the feed holes.
Preferably, the frame comprises a base plate and a pair of cutting groove plate brackets fixed on the base plate, grooves are formed on opposite surfaces of the two cutting groove plate brackets, and the grooves are used for placing the transverse positioning cutting groove plates and the longitudinal positioning cutting groove plates.
Preferably, the locking mechanism comprises a clamping block, the clamping block is slidably fixed in the groove, the clamping block slides through the driving of an electromagnet arranged in the cutting groove plate bracket, and the sliding direction of the clamping block is perpendicular to two side walls of the groove, so that the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are clamped or loosened.
Preferably, the clamping surface of the longitudinal positioning cutting groove plate is provided with an outwards protruding boss, and the clamping surface of the transverse positioning cutting groove plate is provided with a positioning groove at the position corresponding to the boss, wherein the size and the shape of the positioning groove are matched with those of the boss.
Preferably, the blade of the cutter mechanism is a circular cutter.
Preferably, the two pairs of cutter mechanisms comprise a longitudinal cutter mechanism and a transverse cutter mechanism, the longitudinal cutter mechanism matched with the longitudinal positioning cutting groove plate is used for longitudinally cutting the umbilical cord glue, and the transverse cutter mechanism matched with the transverse positioning cutting groove plate is used for transversely cutting the umbilical cord glue.
Preferably, the longitudinal cutter mechanism comprises: the device comprises a longitudinal circular knife, a longitudinal knife rest, a driving motor, a feeding mechanism and a lifting mechanism; the longitudinal tool holder comprises: a rotating shaft, a connecting arm and a connecting rod; the two ends of the rotating shaft are respectively connected with the two connecting arms in a rotating way through bearings, the other ends of the connecting arms are respectively fixed with the connecting rods, longitudinal circular cutters are fixed on the rotating shaft at intervals, the distance and the number of the longitudinal circular cutters are consistent with those of the longitudinal cutter feeding holes, the connecting rods are fixed on a moving pair of the feeding mechanism, the feeding mechanism drives the longitudinal cutter holder to be inserted into or separated from the longitudinal cutter feeding holes, the feeding mechanism is fixed on the moving pair of the lifting mechanism, the feeding mechanism is driven by the lifting mechanism to drive the longitudinal cutter holder to longitudinally cut, and an output shaft of the driving motor is connected with the rotating shaft.
Preferably, the transverse cutting tool mechanism comprises: the device comprises a transverse circular knife, a transverse knife rest, a driving motor, a feeding mechanism and a translation mechanism; the transverse blade holder comprises: a rotating shaft, a connecting arm and a connecting rod; the two ends of the rotating shaft are respectively connected with the two connecting arms in a rotating way through bearings, the other ends of the connecting arms are fixed with connecting rods, transverse circular knives are fixed on the rotating shaft at intervals, the distance and the quantity of the transverse circular knives are consistent with those of the transverse feed holes, the connecting rods are fixed on a moving pair of the feeding mechanism, the feeding mechanism drives the transverse knife rest to be inserted into or separated from the transverse feed holes, the feeding mechanism is arranged on the moving pair of the translation mechanism, the feeding mechanism is driven by the translation mechanism to drive the transverse knife rest to transversely cut, and an output shaft of the driving motor is connected with the rotating shaft.
Preferably, the feeding mechanism, the lifting mechanism and the translation mechanism are all screw transmission mechanisms; the feeding mechanism, the lifting mechanism and the translation mechanism respectively comprise: the device comprises a stepping motor, a screw rod, a sliding rail, a frame and a moving pair; wherein, lead screw and slide rail parallel arrangement, the lead screw is located between two slide rails, and the both ends of slide rail are fixed through the frame respectively, and the lead screw both ends are fixed on the frame through the bearing, and step motor's output shaft is connected with lead screw one end, and the sliding pair is fixed on the slide rail and with lead screw threaded connection.
The automatic umbilical cord Huatong glue dicer can overcome the influence of low efficiency, uneven manual cutting size and unevenness caused by manual fatigue, so that the umbilical cord Huatong glue is cut smoothly, regularly and efficiently; because the umbilical cord Huatong glue is cut by the group of blades, the side length of the cut sheet body is effectively controlled, and the working efficiency is greatly improved; in addition, the invention replaces manual operation by the power device and the transmission device, thereby not only overcoming the problem of high cutting strength caused by extremely strong toughness of the umbilical cord Huatong rubber, but also improving the cutting efficiency.
Description of the drawings:
fig. 1 is a schematic perspective view of an umbilical cord hua tong gum automatic dicer according to a preferred embodiment.
Fig. 2 is a schematic top view of the umbilical cord hua tong automatic dicer according to the preferred embodiment.
Fig. 3 is a schematic perspective view of a longitudinal cutter mechanism according to a preferred embodiment.
Fig. 4 is a schematic perspective view of a preferred embodiment of the transverse cutter mechanism.
Fig. 5 is a schematic perspective view of a transverse positioning cutting slot plate according to a preferred embodiment.
Fig. 6 is another angular perspective view of a preferred embodiment of a transversely oriented cutting slot plate.
Fig. 7 is a schematic perspective view of a longitudinally oriented cutting slot board according to a preferred embodiment.
Fig. 8 is another angular perspective view of a longitudinally oriented cutting slot plate of a preferred embodiment.
In the figure: the machine frame 1, the cutter mechanism 2, the transverse positioning cutting groove plate 4, the longitudinal positioning cutting groove plate 5, the locking mechanism 7, the feed hole 41, the feed hole 51, the clamping surface 42, the clamping surface 52, the groove 43, the groove 53, the bottom plate 11, the cutting groove plate bracket 12, the groove 13, the clamping block 71, the boss 54, the positioning groove 44, the longitudinal cutting cutter mechanism 30, the transverse cutting cutter mechanism 20, the longitudinal circular cutter 31, the longitudinal cutter frame 32, the driving motor 33, the feeding mechanism 34, the lifting mechanism 35, the rotating shaft 321, the connecting arm 322, the connecting rod 323, the transverse circular cutter 21, the transverse cutter frame 22, the driving motor 23, the feeding mechanism 24, the translation mechanism 25, the rotating shaft 221, the connecting arm 222, the connecting rod 223, the stepping motor 351, the lead screw 352, the slide rail 353, the frame 354, and the moving pair 355.
The specific embodiment is as follows:
as shown in fig. 1 to 8, an umbilical cord hua tong automatic dicer comprises: the cutting machine comprises a frame 1, a cutter mechanism 2, a transverse positioning cutting groove plate 4 and a longitudinal positioning cutting groove plate 5; the machine frame 1 is used for fixedly arranging a cutter mechanism 2, a transverse positioning cutting groove plate 4 and a longitudinal positioning cutting groove plate 5, the transverse positioning cutting groove plate 4 and the longitudinal positioning cutting groove plate 5 are used for clamping umbilical cord glue, the transverse positioning cutting groove plate 4 and the longitudinal positioning cutting groove plate 5 are locked through a locking mechanism 7, the locking mechanism 7 provides a force for mutually clamping the transverse positioning cutting groove plate 4 and the longitudinal positioning cutting groove plate 5, a group of transverse feed holes 41 are distributed on the transverse positioning cutting groove plate 4, a group of longitudinal feed holes 51 are distributed on the longitudinal positioning cutting groove plate, the feed holes 41 and 51 are strip-shaped through holes, clamping faces 42 and 52 of the transverse positioning cutting groove plate 4 and the longitudinal positioning cutting groove plate 5 are respectively provided with grooves 43 and 53, the grooves 43 of the longitudinal positioning cutting groove plate 5 correspond to the transverse feed holes 41, the grooves and the feed holes on the groove plate form a longitudinal and transverse feed holes 41, the cutter mechanism 2 is used for automatically completing cutting and backing actions, the blades on the cutter mechanism 2 are provided with a row of blades which are arranged at equal intervals and are inserted and backing.
In the present embodiment, the frame 1 includes a base plate 11 and a pair of cutting board holders 12 fixed to the base plate 11, and grooves 13 are formed in opposite sides of the two cutting board holders 12, and the grooves 13 are used for placing the transverse positioning cutting board 4 and the longitudinal positioning cutting board 5.
In the present embodiment, the locking mechanism 7 includes the clamp block 71, the clamp block 71 is slidably fixed in the groove 13, the clamp block 71 is slid by the driving of the electromagnet provided in the cutting groove plate holder 12, and the sliding direction of the clamp block 71 is perpendicular to both side walls of the groove 13 to clamp or unclamp the lateral positioning cutting groove plate 4 and the longitudinal positioning cutting groove plate 5.
In this embodiment, the clamping surface 53 of the longitudinal positioning cutting groove plate 5 is provided with a boss 54 protruding outwards, the clamping surface 43 of the transverse positioning cutting groove plate 4 is provided with a positioning groove 44 corresponding to the boss 54, and the size and shape of the positioning groove 44 are matched with those of the boss 54.
In the present embodiment, the blade of the cutter mechanism 2 is a circular cutter.
In this embodiment, the two pairs of cutter mechanisms 2 include a longitudinal cutter mechanism 30 and a transverse cutter mechanism 20, and the longitudinal cutter mechanism 30 is matched with the longitudinal positioning cutting groove plate 5 to longitudinally cut the umbilical cord glue, and the transverse cutter mechanism 20 is matched with the transverse positioning cutting groove plate 4 to transversely cut the umbilical cord glue.
In the present embodiment, the longitudinal cutter mechanism 30 includes: a longitudinal circular knife 31, a longitudinal knife rest 32, a driving motor 33, a feeding mechanism 34 and a lifting mechanism 35; the longitudinal tool holder 32 includes: a rotation shaft 321, a connection arm 322, and a connection rod 323; wherein, two ends of the rotating shaft 321 are respectively connected with two connecting arms 322 in a rotating way through bearings, the other ends of the connecting arms 322 are respectively fixed with connecting rods 323, longitudinal circular knives 31 are fixed on the rotating shaft 321 at intervals, the distance and the number of the longitudinal circular knives 31 are consistent with those of the longitudinal feed holes 51, the connecting rods 323 are fixed on a moving pair of the feeding mechanism 34, the feeding mechanism 34 drives the longitudinal knife rest 32 to be inserted into or separated from the longitudinal feed holes 51, the feeding mechanism 34 is fixed on the moving pair of the lifting mechanism 35, and the lifting mechanism 35 drives the feeding mechanism 34 to drive the longitudinal knife rest 32 to longitudinally cut, and an output shaft of the driving motor 33 is connected with the rotating shaft 321.
In the present embodiment, the lateral cutter mechanism 20 includes: a transverse circular knife 21, a transverse knife rest 22, a driving motor 23, a feeding mechanism 24 and a translation mechanism 25; the lateral blade holder 22 includes: a rotation shaft 221, a connection arm 222, and a connection rod 223; wherein, the two ends of the rotation shaft 221 are respectively connected with two connection arms 222 in a rotating way through bearings, the other ends of the connection arms 222 are fixed with a connecting rod 223, transverse circular knives 21 are fixed on the rotation shaft 221 at intervals, the distance and the number of the transverse circular knives 21 are consistent with those of the transverse feed holes 41, the connecting rod 223 is fixed on a moving pair of the feeding mechanism 24, the feeding mechanism 24 drives the transverse knife rest 22 to be inserted into or separated from the transverse feed holes 41 through the feeding mechanism 24, the feeding mechanism 24 drives the feeding mechanism 24 through the translation mechanism 25 to drive the transverse knife rest 22 to transversely cut, and an output shaft of the driving motor 23 is connected with the rotation shaft 221.
In the present embodiment, the feeding mechanisms 34, 24, the lifting mechanism 35 and the translation mechanism 25 are all screw drive mechanisms; the feeding mechanisms 34, 24, the lifting mechanism 35, and the translation mechanism 25 each include (only the lifting mechanism 35 is illustrated here): a stepping motor 351, a screw 352, a slide rail 353, a frame 354 and a moving pair 355; wherein, lead screw 352 and slide rail 353 parallel arrangement, lead screw 352 are located between two slide rails 353, and the both ends of slide rail 353 are fixed through frame 354 respectively, and the lead screw 352 both ends are fixed on frame 354 through the bearing, and step motor 351's output shaft is connected with lead screw 352 one end, and the sliding pair 355 slide-fix on slide rail 353 and with lead screw 352 threaded connection.

Claims (9)

1. An automatic slabbing machine of navel cord hua tong jiao, its characterized in that: comprising the following steps: the device comprises a frame, a cutter mechanism, a transverse positioning cutting groove plate and a longitudinal positioning cutting groove plate; the machine frame is used for fixedly arranging a cutter mechanism, a transverse positioning cutting groove plate and a longitudinal positioning cutting groove plate, the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are used for clamping umbilical cord glue, the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are locked through a locking mechanism, the locking mechanism provides force for mutually clamping the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate, a group of transverse feed holes are distributed on the transverse positioning cutting groove plate, a group of longitudinal feed holes are distributed on the longitudinal positioning cutting groove plate, the feed holes are strip-shaped through holes, grooves are respectively formed in clamping surfaces of the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate, the grooves of the longitudinal positioning cutting groove plate correspond to the longitudinal feed holes, the cutter mechanism is used for automatically completing feeding, cutting and retracting actions, and a row of blades which are arranged at equal intervals are arranged on a blade supporting rod of the cutter mechanism and extend/retract intention blades into/retract from the feed holes.
2. The automatic umbilical cord hua tong gum dicer as claimed in claim 1, wherein: the frame includes the bottom plate and fixes the cutting frid support on the bottom plate, and the cutting frid support is a pair of, opens to have the groove on the face that two cutting frid supports are relative, and this groove is used for placing horizontal location cutting frid and vertical location cutting frid.
3. The automatic umbilical cord hua tong gum dicer as claimed in claim 2, wherein: the locking mechanism comprises a clamping block, the clamping block is slidably fixed in the groove, the clamping block slides through the driving of an electromagnet arranged in the cutting groove plate bracket, and the sliding direction of the clamping block is perpendicular to the two side walls of the groove, so that the transverse positioning cutting groove plate and the longitudinal positioning cutting groove plate are clamped or loosened.
4. An automatic umbilical cord hua tong gum dicer as claimed in any one of claims 1 to 3 wherein: the clamping surface of the longitudinal positioning cutting groove plate is provided with an outwards protruding boss, and the position of the clamping surface of the transverse positioning cutting groove plate corresponding to the boss is provided with a positioning groove, and the size and the shape of the positioning groove are matched with those of the boss.
5. The automatic umbilical cord hua tong gum dicer as claimed in claim 1, wherein: the blade of the cutter mechanism is a circular cutter.
6. The automatic umbilical cord hua tong gum dicer as claimed in claim 1, wherein: the two pairs of cutter mechanisms comprise a longitudinal cutter mechanism and a transverse cutter mechanism, the longitudinal cutter mechanism matched with the longitudinal positioning cutting groove plate is used for longitudinally cutting the umbilical cord glue, and the transverse cutter mechanism matched with the transverse positioning cutting groove plate is used for transversely cutting the umbilical cord glue.
7. The automatic umbilical cord hua tong gum dicer as claimed in claim 6, wherein: the longitudinal cutting tool mechanism comprises: the device comprises a longitudinal circular knife, a longitudinal knife rest, a driving motor, a feeding mechanism and a lifting mechanism; the longitudinal tool holder comprises: a rotating shaft, a connecting arm and a connecting rod; the two ends of the rotating shaft are respectively connected with the two connecting arms in a rotating way through bearings, the other ends of the connecting arms are respectively fixed with connecting rods, longitudinal circular knives are fixed on the rotating shaft at intervals, the distance and the number of the longitudinal circular knives are consistent with those of longitudinal feed holes, the connecting rods are fixed on a moving pair of the feeding mechanism, the feeding mechanism drives the longitudinal knife rest to be inserted into or separated from the longitudinal feed holes, the feeding mechanism is fixed on the moving pair of the lifting mechanism, the feeding mechanism is driven by the lifting mechanism to drive the longitudinal knife rest to longitudinally cut, and an output shaft of the driving motor is connected with the rotating shaft.
8. The automatic umbilical cord hua tong gum dicer as claimed in claim 6, wherein: the transverse cutting tool mechanism includes: the device comprises a transverse circular knife, a transverse knife rest, a driving motor, a feeding mechanism and a translation mechanism; the transverse blade holder comprises: a rotating shaft, a connecting arm and a connecting rod; the two ends of the rotating shaft are respectively connected with the two connecting arms in a rotating way through bearings, the other ends of the connecting arms are fixed with connecting rods, transverse circular knives are fixed on the rotating shaft at intervals, the distance and the quantity of the transverse circular knives are consistent with those of the transverse feed holes, the connecting rods are fixed on a moving pair of the feeding mechanism, the feeding mechanism drives the transverse knife rest to be inserted into or separated from the transverse feed holes, the feeding mechanism is arranged on the moving pair of the translation mechanism, the feeding mechanism is driven by the translation mechanism to drive the transverse knife rest to transversely cut, and an output shaft of the driving motor is connected with the rotating shaft.
9. The automatic umbilical cord hua tong gum dicer as claimed in claim 7 or claim 8, wherein: the feeding mechanism, the lifting mechanism and the translation mechanism are all screw transmission mechanisms; the feeding mechanism, the lifting mechanism and the translation mechanism respectively comprise: the device comprises a stepping motor, a screw rod, a sliding rail, a frame and a moving pair; wherein, lead screw and slide rail parallel arrangement, the lead screw is located between two slide rails, and the both ends of slide rail are fixed through the frame respectively, and the lead screw both ends are fixed on the frame through the bearing, and step motor's output shaft is connected with lead screw one end, and the sliding pair is fixed on the slide rail and with lead screw threaded connection.
CN201510521993.1A 2015-08-24 2015-08-24 Automatic umbilical cord Huatong glue dicer Active CN105269615B (en)

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CN101870116A (en) * 2010-05-17 2010-10-27 北京西贝利德科贸有限公司 Medicinal cutter
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