CN103289891B - Fast bovine oocyte collector - Google Patents
Fast bovine oocyte collector Download PDFInfo
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- CN103289891B CN103289891B CN201310168939.4A CN201310168939A CN103289891B CN 103289891 B CN103289891 B CN 103289891B CN 201310168939 A CN201310168939 A CN 201310168939A CN 103289891 B CN103289891 B CN 103289891B
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- ovocyte
- guide groove
- acupuncture treatment
- oocyte
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Abstract
The invention discloses a fast bovine oocyte collector which comprises a base, wherein a support frame is fixed at the center of the base; the upper part of the support frame is provided with a fixing bracket component in sliding cup-joint fit therewith; a cross rod is fixed at the other end of the support frame; a motor is fixed below the other end of the cross rod; an oocyte collection cup is fixed at the other end of the fixing bracket; a transmission shaft of the motor is fixedly connected with a transmission rod; a fixing claw is fixed at the other end of the transmission rod; the fixing claw is located in the oocyte collection cup; and the lower end of the oocyte collection cup is placed in a water bath kettle. The fast bovine oocyte collector disclosed by the invention has a simple structure, is convenient to use, and can greatly improve the bovine oocyte collection efficiency, improve the quality of the collected oocyte and reduce the working intensity of oocyte collection.
Description
Technical field
The invention belongs to embryo's biological study apparatus field, be specifically related to a kind of bovine oocyte Quick Acquisition device.
Background technology
Develop rapidly along with current biotechnology; the field of the biological research of embryo and application is also in continuous expansion; and start to be applied to the livestock industry such as ox is produced and research in, in the research and production such as in vitro fertilization, transgenic animal, nuclear transplantation, external embryo's large-scale production, the demand of ovocyte is increased.The source of bovine oocyte mainly contains two, a kind of is to utilize modus operandi or B-ultrasound method directly from live body ox ovary, to extract, another kind of extensively, a large amount of and cheap source is collection to be butchered to the ovary of rear ox, take back laboratory or embryo workshop and concentrate the ovocyte gathering in ovarian follicle above.
From butchering, on the ovary of ox, acquisition method mainly contains three kinds at present:
1. ovarian follicle extraction method: draw one by one liquor folliculi and the ovocyte on ovary surface with syringe from ovary inside, the liquor folliculi of extraction is found out to ovocyte after staticly settling under stereomicroscope;
2. ovary sectility method: cut brokenly ovary surface ovarian follicle with blade, after washing fluid rinses, after rushing ovum liquid bottom settlings, extract bottom settlings ovocyte and find out ovocyte under stereomicroscope;
3. Follicle puncture method, the ovarian follicle with the punctures ovary surface of blunt, slowly flows into containing in the culture dish of basic culture solution liquor folliculi.Under stereomicroscope, collect ovarian cumulus-ovocyte complex body (COCs).
The acquisition method of above-mentioned all kinds of ovocytes result of use is in practice not quite similar, and obtain a fairly large number of oocyte number, also will consider the efficiency of collection and the integrity of cumulus cell simultaneously.
Through many experiments comparison, the Oocyte quality that the method for analysing obtains is better, and the time spending is short.Therefore, design a kind of postmortem method bovine oocyte that utilizes and gather apparatus, quality and the speed of for improving bovine oocyte, collecting, for the batch production of ox IVF Embryos, produce and provide useful apparatus to become the task of top priority.
Summary of the invention
The object of the invention aims to provide oocyte collection efficiency on a kind of ox ovary that can improve fast from butchering, and improve the bovine oocyte Quick Acquisition device of the quality of the ovocyte collect, industrialization, the batch production that it can effectively realize ox IVF Embryos produce external embryo simultaneously can also for academic research provide a kind of simple, convenient, efficiently, the collection apparatus that can ensure the quality of products again.
In order to solve existing problem in background technology, it comprises base, the circle centre position of this base is fixed with bracing frame, the top of this bracing frame is provided with it and is slidably socketed the m coordinating, the other end of support frame as described above is fixed with cross bar, the below of the other end of this cross bar is fixed with motor, described support bracket fastened other one end is fixed with ovocyte acquisition cup, the transmission shaft of described motor is fixedly connected with transmission rod, the other end of this transmission rod is fixed with fixed jaw, described fixed jaw is positioned at ovocyte acquisition cup, the lower end of described ovocyte acquisition cup is arranged in water-bath.
Described transmission rod is divided into uploads lever and underdrive bar, described lower end of uploading lever inwardly mills out mortise, the upper end of described underdrive bar mills out the tenon of pegging graft and coordinating with mortise, between described mortise and tenon, mill out concentric screw, in this screw, be inserted with bearing pin, described uploading between lever and underdrive bar is rotatably assorted, described in upload lever outer surface cover have cutting ferrule, on described underdrive bar, be fixed with a circle spacing ring.
Described m comprises tooth bar and the adjusting gear being meshed with it and the sleeve pipe that is welded on bracing frame inner side, described casing pipe sleeve is on bracing frame, described adjusting gear is arranged in sleeve pipe, on its gear hole, adjusting knob is installed, one end of described sleeve pipe is provided with fixed knob, its the other end is fixed with glass holder, and the middle part of this glass holder is provided with the hole that fits tightly ovocyte acquisition cup external diameter.
The bottom of described ovocyte acquisition cup is conical structure, and its top one side offers step.
Described fixed jaw integral body is ellipsoidal structure, the mid-way of its bottom is fixed with acupuncture treatment, the both sides mirror image of this acupuncture treatment is provided with guide groove, in this guide groove, be provided with the mobile acupuncture treatment being slidably matched with it, the inner side of described guide groove is provided with adjusting chamber one, in this adjusting chamber, on the sidewall relative with guide groove, evenly offer the fixed orifices of pegging graft and coordinating with mobile acupuncture treatment tail end, the tail end of described mobile acupuncture treatment extend into and regulates in chamber and near its tail end, be provided with a circle back-up ring, between this back-up ring and guide groove, be provided with the spring being enclosed within mobile acupuncture treatment, one end of this spring withstands on back-up ring, its the other end withstands on the inner side of guide groove.
An ovarian follicle scribe knife, it comprises handle of a knife, and one end of this handle of a knife is provided with tool rest, and other one end of this tool rest is arranged with slot, is inserted with and draws a cutter in this slot, and the other end of described stroke of cutter is inwardly curved goes out hook formation.
The described distance of drawing between cutter is 1-1.5mm.
Owing to having adopted above technical scheme, the present invention has following beneficial effect: simple in structure, easy to use, can greatly improve the collecting efficiency of bovine oocyte, and improve the quality of collected ovocyte, alleviate the working strength of oocyte collection.
Accompanying drawing explanation
In order to be illustrated more clearly in the present invention, below in conjunction with accompanying drawing, embodiment is briefly described.
Fig. 1 is one-piece construction schematic diagram of the present invention;
Fig. 2 is the one-piece construction schematic diagram of ovarian follicle scribe knife in the present invention;
Fig. 3 is the structural representation of transmission rod in the present invention;
Fig. 4 is the structural representation of fixed jaw in the present invention.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described.
Referring to Fig. 1, this embodiment is to be achieved by the following technical solutions, it comprises base 1, the circle centre position of this base 1 is fixed with bracing frame 2, the top of this bracing frame 2 is provided with it and is slidably socketed the m 3 coordinating, the other end of support frame as described above 2 is fixed with cross bar 4, the below of the other end of this cross bar 4 is fixed with motor 5, other one end of described fixed support 3 is fixed with ovocyte acquisition cup 6, the transmission shaft of described motor 5 is fixedly connected with transmission rod 7, the other end of this transmission rod 7 is fixed with fixed jaw 8, described fixed jaw 8 is positioned at ovocyte acquisition cup 6, the lower end of described ovocyte acquisition cup 6 is arranged in water-bath 9.
Referring to Fig. 3, described transmission rod 7 is divided into uploads lever 71 and underdrive bar 72, described lower end of uploading lever 71 inwardly mills out mortise, the upper end of described underdrive bar 72 mills out the tenon of pegging graft and coordinating with mortise, between described mortise and tenon, mill out concentric screw, in this screw, be inserted with bearing pin, described uploading between lever 71 and underdrive bar 72 is rotatably assorted, the described outer surface cover of uploading lever 71 has cutting ferrule 73, is fixed with a circle spacing ring 74 on described underdrive bar 72.
Referring to Fig. 1, described m 3 comprises and is welded on the tooth bar 31 of bracing frame 2 inner sides and the adjusting gear being meshed with it and sleeve pipe 32, described sleeve pipe 32 is enclosed within on bracing frame 2, described adjusting gear is arranged in sleeve pipe 32, adjusting knob 33 is installed on its gear hole, one end of described sleeve pipe 32 is provided with fixed knob 34, and its other end is fixed with glass holder 35, and the middle part of this glass holder 35 is provided with the hole that fits tightly ovocyte acquisition cup 6 external diameters.
Referring to Fig. 1, the bottom of described ovocyte acquisition cup 6 is conical structure, and its top one side offers step 11.
Referring to Fig. 4, described fixed jaw 8 integral body are ellipsoidal structure, the mid-way of its bottom is fixed with acupuncture treatment 81, the both sides mirror image of this acupuncture treatment 81 is provided with guide groove, in this guide groove, be provided with the mobile acupuncture treatment 82 being slidably matched with it, the inner side of described guide groove is provided with adjusting chamber 83 one, on this interior sidewall relative with guide groove in adjusting chamber 83, evenly offer the fixed orifices 84 of pegging graft and coordinating with mobile acupuncture treatment 82 tail ends, the tail end of described mobile acupuncture treatment 82 extend into and regulates in chamber 83 and near its tail end, be provided with a circle back-up ring 85, between this back-up ring 85 and guide groove, be provided with the spring 86 being enclosed within mobile acupuncture treatment 82, one end of this spring 86 withstands on back-up ring 85, its the other end withstands on the inner side of guide groove.
Referring to Fig. 2, a kind of ovarian follicle scribe knife, it comprises handle of a knife 10, and one end of this handle of a knife 10 is provided with tool rest 101, and other one end of this tool rest 101 is arranged with slot, is inserted with and draws a cutter 102 in this slot, and the other end of described stroke of cutter 102 is inwardly curved goes out hook formation.
The described distance of drawing between cutter 102 is 1-1.5mm.
During use, first in water-bath 9, pour water into, and make the water temperature in water-bath 9 keep constant temperature at 37-38 ℃, in ovocyte acquisition cup 6, pour again egg-cleaning liquid into step 11 places, and the temperature of the egg-cleaning liquid in ovocyte acquisition cup 6 and the water temperature in water-bath 9 are consistent, then mention cutting ferrule 73, rotate underdrive bar 72 to suitable position, then according to two mobile acupuncture treatments 82 of large young pathbreaker of ovary, be adjusted to appropriate position with acupuncture treatment 81, during adjusting, pull out mobile acupuncture treatment 82, and unclamp after bringing it into suitable position, the tail end of mobile acupuncture treatment 82 is inserted in fixed orifices 84, then ovary is firmly pricked on three pins, underdrive bar 72 is vertically put down, and it is fixing to put down cutting ferrule 73, then by regulating bracket component 3 that ovary is adjusted to the egg-cleaning liquid top with ovocyte acquisition cup 6 step 11 places, hand-held female bubble scribe knife, utilize the crotch portion of this cutter that ovary surface is all scratched, then ovary surface all being scratched is immersed in and in egg-cleaning liquid, starts motor, the liquor folliculi and the ovocyte that slowly rotate flushing ovary surface enter into egg-cleaning liquid, after punching first, regulate bracket component 3 to rise near the top of ovocyte acquisition cup 6, cutting ferrule 73 is mentioned, underdrive bar 72 is gone to appropriate position, the ovary of excision after cleaning, after finally grade precipitates completely, can from throw out, detect by microscope ovocyte, in washing ovum process, when occurring that the interior settling of ovocyte acquisition cup 6 is more, interchangeable another ovocyte acquisition cup 6 continues operation, and the ovocyte acquisition cup 6 of changing, after precipitation is also centrifugal, can be detected by microscope to ovocyte from throw out.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art should be appreciated that its technical scheme that still can record aforementioned each embodiment modifies, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (5)
1. bovine oocyte Quick Acquisition device, it is characterized in that it comprises base, the circle centre position of this base is fixed with bracing frame, the top of this bracing frame is provided with it and is slidably socketed the m coordinating, the other end of support frame as described above is fixed with cross bar, the below of the other end of this cross bar is fixed with motor, described support bracket fastened other one end is fixed with ovocyte acquisition cup, the transmission shaft of described motor is fixedly connected with transmission rod, the other end of this transmission rod is fixed with fixed jaw, described fixed jaw is positioned at ovocyte acquisition cup, the lower end of described ovocyte acquisition cup is arranged in water-bath.
2. bovine oocyte Quick Acquisition device according to claim 1, transmission rod described in it is characterized in that is divided into uploads lever and underdrive bar, described lower end of uploading lever inwardly mills out mortise, the upper end of described underdrive bar mills out the tenon of pegging graft and coordinating with mortise, between described mortise and tenon, mill out concentric screw, in this screw, be inserted with bearing pin, described uploading between lever and underdrive bar is rotatably assorted, the described outer surface cover of uploading lever has cutting ferrule, is fixed with a circle spacing ring on described underdrive bar.
3. bovine oocyte Quick Acquisition device according to claim 1, it is characterized in that described m comprises tooth bar and the adjusting gear being meshed with it and the sleeve pipe that is welded on bracing frame inner side, described casing pipe sleeve is on bracing frame, described adjusting gear is arranged in sleeve pipe, on its gear hole, adjusting knob is installed, one end of described sleeve pipe is provided with fixed knob, and its other end is fixed with glass holder, and the middle part of this glass holder is provided with the hole that fits tightly ovocyte acquisition cup external diameter.
4. bovine oocyte Quick Acquisition device according to claim 1, the bottom that it is characterized in that described ovocyte acquisition cup is conical structure, its top one side offers step.
5. bovine oocyte Quick Acquisition device according to claim 1, it is characterized in that described fixed jaw integral body is ellipsoidal structure, the mid-way of its bottom is fixed with acupuncture treatment, the both sides mirror image of this acupuncture treatment is provided with guide groove, in this guide groove, be provided with the mobile acupuncture treatment being slidably matched with it, the inner side of described guide groove is provided with adjusting chamber one, in this adjusting chamber, on the sidewall relative with guide groove, evenly offer the fixed orifices of pegging graft and coordinating with mobile acupuncture treatment tail end, the tail end of described mobile acupuncture treatment extend into and regulates in chamber and near its tail end, be provided with a circle back-up ring, between this back-up ring and guide groove, be provided with the spring being enclosed within mobile acupuncture treatment, one end of this spring withstands on back-up ring, its the other end withstands on the inner side of guide groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310168939.4A CN103289891B (en) | 2013-04-23 | 2013-04-23 | Fast bovine oocyte collector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310168939.4A CN103289891B (en) | 2013-04-23 | 2013-04-23 | Fast bovine oocyte collector |
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| CN103289891A CN103289891A (en) | 2013-09-11 |
| CN103289891B true CN103289891B (en) | 2014-11-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310168939.4A Expired - Fee Related CN103289891B (en) | 2013-04-23 | 2013-04-23 | Fast bovine oocyte collector |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105838581B (en) * | 2016-05-11 | 2017-11-10 | 安徽农业大学 | A kind of animal ovary egg mother cell separates and collects device |
| CN106085829B (en) * | 2016-07-06 | 2018-02-16 | 上海海洋大学 | A kind of egg mother cell accumulator |
| CN109182104B (en) * | 2018-11-08 | 2023-11-03 | 上海交通大学医学院附属瑞金医院 | Full-automatic follicular needling machine |
| CN109929759B (en) * | 2019-03-22 | 2021-07-20 | 山东农业大学 | Collection and early-stage forming culture device for oocyte |
| CN109852533B (en) * | 2019-04-02 | 2022-02-25 | 泰山医学院 | High-efficiency oocyte separation and collection device for animal ovary |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110004120A1 (en) * | 2009-05-28 | 2011-01-06 | Angiotech Pharmaceuticals Inc. | Biopsy device needle set |
| CN201411459Y (en) * | 2009-06-07 | 2010-02-24 | 赛海芳 | Adjustable air-deposited bacterium sampling frame |
| CN101709290B (en) * | 2009-12-03 | 2012-10-03 | 安徽农业大学 | Simple, economic and efficient method for in-vitro maturity of porcine oocytes |
| CN102670284A (en) * | 2011-03-15 | 2012-09-19 | 浙江海洋学院 | Forked puncture trocar |
| CN202155164U (en) * | 2011-06-30 | 2012-03-07 | 浙江大之医药胶囊有限公司 | Release agent preparing device |
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