CN111004710B - Plant tissue sterile extractor based on transgenic technology - Google Patents
Plant tissue sterile extractor based on transgenic technology Download PDFInfo
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- CN111004710B CN111004710B CN201911361246.0A CN201911361246A CN111004710B CN 111004710 B CN111004710 B CN 111004710B CN 201911361246 A CN201911361246 A CN 201911361246A CN 111004710 B CN111004710 B CN 111004710B
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- tissue
- seat
- extraction kettle
- fluid extraction
- plant tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
Abstract
The invention discloses a plant tissue sterile extractor based on a transgenic technology, which is characterized in that a supporting and moving mechanism is arranged, so that the supporting and moving mechanism can drive a tissue fluid extraction kettle to move between a tissue taking mechanism and a distilled water supply tank so as to respectively butt joint the tissue taking mechanism and the distilled water supply tank, the running water type operation of extraction can be effectively realized, the simplicity is improved, the procedure can be simplified, and the influence possibility of external bacteria is reduced.
Description
Technical Field
The invention relates to a sterile extractor, in particular to a plant tissue sterile extractor based on a transgenic technology.
Background
Currently, in order to achieve analysis and research of plants of transgenic technology, it is necessary to extract and analyze their plant tissues aseptically. The current aseptic extraction generally uses an extraction knife to cut plant tissues, then is placed in an aseptic bottle, is crushed by stirring crushing equipment and the like, and is extracted by using an extracting solution, so that the mode is more troublesome, moreover, during extraction, the extraction knife is in contact with the outside, and the problems of errors such as manual operation and the like are solved, the sterility is difficult to truly guarantee, and meanwhile, when the tissues are resected, the size of the tissue blocks is difficult to grasp, the operation is difficult, and the rapid running water type operation cannot be realized, so that the efficiency of experiments and extraction is influenced.
Accordingly, the present invention provides a plant tissue sterile extractor based on transgenic technology to solve the problems set forth in the background art.
Disclosure of Invention
The invention aims to provide a plant tissue sterile extractor based on a transgenic technology, so as to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the plant tissue sterile extractor based on the transgenic technology comprises a tissue fluid extraction kettle, a supporting and moving mechanism, a tissue taking mechanism and a distilled water supply tank, and is characterized in that the tissue fluid extraction kettle is transversely and movably arranged on the supporting and moving mechanism, the tissue taking mechanism and the distilled water supply tank which are transversely arranged on a transverse frame at intervals are arranged above the tissue fluid extraction kettle, and the supporting and moving mechanism can drive the tissue fluid extraction kettle to move between the tissue taking mechanism and the distilled water supply tank so as to respectively butt joint the tissue taking mechanism and the distilled water supply tank; the tissue taking mechanism can automatically take plant tissues into blocks and directly put the plant tissues into the tissue liquid extraction kettle, and the distilled water supply tank can provide sterile distilled water for the tissue liquid extraction kettle.
Further, as the preference, the tissue taking mechanism includes and gets material sword subassembly, plant tissue and hold seat and lift actuating mechanism, wherein, plant tissue holds the seat and fixes on horizontal frame, just be provided with on the horizontal frame with plant tissue holds the through-hole that the chamber communicates that the seat was held to plant tissue, plant tissue holds to be provided with on the seat and stretches into the material sword subassembly that plant tissue held the intracavity, material sword subassembly by lift actuating mechanism drive goes up and down, just plant tissue holds to be located on the seat the plant tissue that gets material sword subassembly is provided with the passageway that supplies the plant tissue of getting material to fall into in the tissue liquid extraction cauldron under.
Further, as the preference, plant tissue holds and is provided with the big lower extreme of vertical upper end little taper sleeve on the seat, get material sword subassembly and include blade holder and preceding tool bit, the upper end fixed connection of blade holder to lift actuating mechanism, the bottom fixed connection of blade holder preceding tool bit, blade holder and preceding tool bit are located in the taper sleeve, just the lower extreme of preceding tool bit is provided with the slotted hole of indent.
Further preferably, the slot is of an inverted U-shaped structure.
Further, as the preference, lift actuating mechanism includes drive seat, interior piston, push rod and guide bar, wherein, the drive seat is fixed on the horizontal frame, sealed slip is provided with interior piston in the drive seat, the lower extreme of interior piston is connected the push rod, the upper end of interior piston is provided with stretches out the guide bar outside the drive seat.
Further, preferably, a smashing and stirring mechanism is arranged in the tissue fluid extraction kettle, the smashing and stirring mechanism comprises smashing leaves and a circulating flow injection mechanism, the smashing leaves are rotatably fixed at the center of the tissue fluid extraction kettle, the circulating flow injection mechanism is arranged at the bottom of the tissue fluid extraction kettle, and the circulating flow injection mechanism can enable liquid in the tissue fluid extraction kettle to be sprayed to the smashing leaves.
Further, as the preference, the top of tissue liquid extraction cauldron is provided with the receiving mouth, smash and rabbling mechanism includes circulating pump, drain pipe, injection cover and a plurality of injection head, wherein, the circulating pump is located on the support of tissue liquid extraction cauldron bottom, the circulating pump input is connected to through the drain pipe in the tissue liquid extraction cauldron, the circulating pump output is connected to the injection cover through the injection pipe, the injection cover is fixed the interior diapire of tissue liquid extraction cauldron, the injection is sheathe in a plurality of injection heads of intercommunication, and each injection head all is towards smashing the leaf.
Further, as the preference, support moving mechanism includes base, lead screw, sliding seat, driving motor, support fixed connection through the bearing frame on the base the lead screw, the lead screw by driving motor drive rotates, slidable is provided with the sliding seat on the lead screw, the tissue fluid draws the cauldron and fixes through the support of bottom on the sliding seat.
Further, preferably, a sliding groove is provided in the middle of the base, and the sliding seat slides along the sliding groove.
Further, preferably, a water outlet control telescopic pipe is connected to the distilled water supply tank, and a disinfection heater for heating, disinfecting and cleaning is further arranged at the bottom of the tissue fluid extraction kettle.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the supporting and moving mechanism is arranged, so that the supporting and moving mechanism can drive the tissue liquid extraction kettle to move between the tissue material taking mechanism and the distilled water supply tank so as to be respectively in butt joint with the tissue material taking mechanism and the distilled water supply tank, the running water type operation of extraction can be effectively realized, the simplicity is improved, the program can be simplified, so that the influence possibility of external bacteria is reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a plant tissue sterile extractor based on transgenic technology;
FIG. 2 is a schematic structural view of a tissue extraction mechanism in a plant tissue sterile extractor based on transgenic technology.
FIG. 3 is a schematic diagram of the internal structure of a tissue fluid extraction kettle of a plant tissue sterile extractor based on transgenic technology;
description of the embodiments
Referring to fig. 1 to 3, in an embodiment of the present invention, a plant tissue sterile extractor based on a transgenic technology includes a tissue fluid extraction kettle 8, a supporting and moving mechanism, a tissue taking mechanism and a distilled water supply tank 16, wherein the tissue fluid extraction kettle 8 is movably disposed on the supporting and moving mechanism, the tissue taking mechanism and the distilled water supply tank 16 which are laterally spaced on a lateral frame 15 are disposed above the tissue fluid extraction kettle, and the supporting and moving mechanism can drive the tissue fluid extraction kettle 8 to move between the tissue taking mechanism and the distilled water supply tank 16 so as to respectively dock the tissue taking mechanism and the distilled water supply tank 16; the tissue taking mechanism can automatically take plant tissues into blocks and directly put the plant tissues into the tissue liquid extraction kettle 8, and the distilled water supply tank 16 can supply sterile distilled water to the tissue liquid extraction kettle 8.
In this embodiment, the tissue taking mechanism comprises a taking knife assembly 12, a plant tissue accommodating seat 10 and a lifting driving mechanism, wherein the plant tissue accommodating seat 10 is fixed on a transverse frame 15, a through hole 11 communicated with a plant tissue accommodating cavity 14 of the plant tissue accommodating seat 10 is arranged on the transverse frame, a taking knife assembly 12 extending into the plant tissue accommodating cavity 14 is arranged on the plant tissue accommodating seat 10, the taking knife assembly 12 is driven to lift by the lifting driving mechanism, and a channel for taking plant tissue falling into a tissue liquid extraction kettle 8 is arranged on the plant tissue accommodating seat 10 under the taking knife assembly 12.
The plant tissue accommodating seat 10 is provided with a vertical conical sleeve 21 with a large upper end and a small lower end, the material taking knife assembly 12 comprises a knife seat 22 and a front knife head 24, the upper end of the knife seat 22 is fixedly connected with the lifting driving mechanism, the bottom of the knife seat is fixedly connected with the front knife head 24, the knife seat 22 and the front knife head 24 are positioned in the conical sleeve 21, the lower end of the front knife head 24 is provided with a concave groove hole 23, and the groove hole 23 is of an inverted U-shaped structure.
The lifting driving mechanism comprises a driving seat 17, an inner piston 19, a push rod 20 and a guide rod 18, wherein the driving seat is fixed on the transverse frame, the inner piston is arranged in the driving seat in a sealing sliding manner, the lower end of the inner piston is connected with the push rod, and the upper end of the inner piston is provided with the guide rod extending out of the driving seat.
The tissue fluid extraction kettle 8 is internally provided with a crushing and stirring mechanism, the crushing and stirring mechanism comprises crushing leaves 7 and a circulating flow injection mechanism, the crushing leaves are rotatably fixed at the center of the tissue fluid extraction kettle 8, the bottom of the tissue fluid extraction kettle 8 is provided with the circulating flow injection mechanism, and the circulating flow injection mechanism can enable liquid in the tissue fluid extraction kettle 8 to be sprayed to the crushing leaves 7.
The top of tissue liquid extraction cauldron 8 is provided with the receiving mouth, smash and rabbling mechanism includes circulating pump 6, drain pipe 27, injection pipe 26, injection cover 25 and a plurality of injection head 9, wherein, the circulating pump is located on the support 4 of tissue liquid extraction cauldron 8 bottom, the circulating pump input is connected to through the drain pipe in the tissue liquid extraction cauldron 8, the circulating pump output is connected to injection cover 25 through injection pipe 26, the injection cover is fixed the interior bottom wall of tissue liquid extraction cauldron 8, the injection is sheathe in and is linked together a plurality of injection heads 9, and each injection head 9 all is towards smashing leaf 7.
The supporting and moving mechanism comprises a base 1, a screw rod 3, a sliding seat 2 and a driving motor 5, wherein the base is fixedly connected with the screw rod through a bearing seat, the screw rod is driven by the driving motor to rotate, the sliding seat is slidably arranged on the screw rod, and the tissue fluid extraction kettle 8 is fixed on the sliding seat through a bracket 4 at the bottom. The middle part of base is provided with the sliding tray, the sliding seat is followed the sliding tray slides.
In the invention, a distilled water supply tank 16 is connected with a water outlet control telescopic pipe, and the bottom of the tissue fluid extraction kettle 8 is also provided with a disinfection heater for heating, disinfecting and cleaning.
According to the invention, the supporting and moving mechanism is arranged, so that the supporting and moving mechanism can drive the tissue liquid extraction kettle to move between the tissue material taking mechanism and the distilled water supply tank so as to be respectively in butt joint with the tissue material taking mechanism and the distilled water supply tank, the running water type operation of extraction can be effectively realized, the simplicity is improved, the program can be simplified, so that the influence possibility of external bacteria is reduced.
The foregoing description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical solution of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (7)
1. The plant tissue sterile extractor based on the transgenic technology comprises a tissue fluid extraction kettle (8), a supporting and moving mechanism, a tissue taking mechanism and a distilled water supply tank (16), and is characterized in that the tissue fluid extraction kettle (8) is transversely movably arranged on the supporting and moving mechanism, the tissue taking mechanism and the distilled water supply tank (16) which are transversely arranged on a transverse frame (15) at intervals are arranged above the tissue fluid extraction kettle, and the supporting and moving mechanism can drive the tissue fluid extraction kettle (8) to move between the tissue taking mechanism and the distilled water supply tank (16) so as to respectively butt joint the tissue taking mechanism and the distilled water supply tank (16); the tissue taking mechanism can automatically take plant tissues into blocks and directly put the plant tissues into the tissue liquid extraction kettle (8), and the distilled water supply tank (16) can provide sterile distilled water for the tissue liquid extraction kettle (8);
the tissue taking mechanism comprises a taking knife assembly (12), a plant tissue accommodating seat (10) and a lifting driving mechanism, wherein the plant tissue accommodating seat (10) is fixed on a transverse frame (15), a through hole (11) communicated with a plant tissue accommodating cavity (14) of the plant tissue accommodating seat (10) is formed in the transverse frame, the plant tissue accommodating seat (10) is provided with the taking knife assembly (12) extending into the plant tissue accommodating cavity (14), the taking knife assembly (12) is driven to lift by the lifting driving mechanism, and a channel for taking plant tissue falling into a tissue liquid extraction kettle (8) is formed on the plant tissue accommodating seat (10) and located under the taking knife assembly (12);
the plant tissue accommodating seat (10) is provided with a vertical conical sleeve (21) with a large upper end and a small lower end, the material taking cutter assembly (12) comprises a cutter seat (22) and a front cutter head (24), the upper end of the cutter seat (22) is fixedly connected to the lifting driving mechanism, the bottom of the cutter seat is fixedly connected with the front cutter head (24), the cutter seat (22) and the front cutter head (24) are positioned in the conical sleeve (21), and the lower end of the front cutter head (24) is provided with a concave slot hole (23);
the tissue fluid extraction kettle (8) is internally provided with a crushing and stirring mechanism, the crushing and stirring mechanism comprises crushing leaves (7) and a circulating flow injection mechanism, the crushing leaves are rotatably fixed at the center of the tissue fluid extraction kettle (8), the bottom of the tissue fluid extraction kettle (8) is provided with the circulating flow injection mechanism, and the circulating flow injection mechanism can enable liquid in the tissue fluid extraction kettle (8) to be sprayed to the crushing leaves (7).
2. The plant tissue sterile extractor based on the transgenic technology according to claim 1, characterized in that the slot (23) is of inverted U-shaped structure.
3. The plant tissue sterile extractor based on the transgenic technology according to claim 1, characterized in that the lifting driving mechanism comprises a driving seat (17), an inner piston (19), a push rod (20) and a guide rod (18), wherein the driving seat (17) is fixed on the transverse frame, the inner piston (19) is arranged in the driving seat (17) in a sealing sliding manner, the lower end of the inner piston (19) is connected with the push rod (20), and the upper end of the inner piston (19) is provided with the guide rod (18) extending out of the driving seat (17).
4. The plant tissue sterile extractor based on the transgenic technology according to claim 1, characterized in that the top of the tissue fluid extraction kettle (8) is provided with a material receiving port, the crushing and stirring mechanism comprises a circulating pump (6), a liquid outlet pipe (27), a spray pipe (26), a spray sleeve (25) and a plurality of spray heads (9), wherein the circulating pump is positioned on a bracket (4) at the bottom of the tissue fluid extraction kettle (8), the input end of the circulating pump is connected into the tissue fluid extraction kettle (8) through the liquid outlet pipe, the output end of the circulating pump is connected to the spray sleeve (25) through the spray pipe (26), the spray sleeve is fixed on the inner bottom wall of the tissue fluid extraction kettle (8), the spray sleeve is communicated with the plurality of spray heads (9), and each spray head (9) faces a crushing blade (7).
5. The plant tissue sterile extractor based on the transgenic technology according to claim 1, wherein the supporting and moving mechanism comprises a base (1), a screw rod (3), a sliding seat (2) and a driving motor (5), the screw rod (3) is fixedly connected to the base (1) through a bearing seat, the screw rod (3) is driven to rotate by the driving motor (5), the sliding seat (2) is slidably arranged on the screw rod (3), and the tissue fluid extraction kettle (8) is fixed to the sliding seat (2) through a bracket (4) at the bottom.
6. A plant tissue sterile extractor based on transgenic technology according to claim 5, characterized in that the middle part of the base (1) is provided with a sliding groove along which the sliding seat (2) slides.
7. The plant tissue sterile extractor based on the transgenic technology according to claim 1, characterized in that a distilled water supply tank (16) is connected with a water outlet control telescopic pipe, and the bottom of the tissue fluid extraction kettle (8) is also provided with a disinfection heater for heating, disinfecting and cleaning.
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CN201911361246.0A CN111004710B (en) | 2019-12-25 | 2019-12-25 | Plant tissue sterile extractor based on transgenic technology |
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CN201911361246.0A CN111004710B (en) | 2019-12-25 | 2019-12-25 | Plant tissue sterile extractor based on transgenic technology |
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CN111004710B true CN111004710B (en) | 2023-09-12 |
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Citations (8)
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TW200708329A (en) * | 2005-08-26 | 2007-03-01 | Yen Chen Machinery Co Ltd | Extracting, concentrating, and manufacturing method of medicine solution |
AU2012207053A1 (en) * | 2006-08-31 | 2012-08-16 | Monsanto Technology Llc | Methods for producing transgenic plants |
CN102847340A (en) * | 2012-09-25 | 2013-01-02 | 湖北民族学院 | Full-automatic traditional Chinese medicinal material ingredient extracting device |
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CN207175918U (en) * | 2017-07-17 | 2018-04-03 | 天津信者生物技术有限公司 | One kind rubs centrifugal plant tissue liquid extraction element |
CN207300608U (en) * | 2017-10-12 | 2018-05-01 | 山东三岳化工有限公司 | A kind of chemical reaction kettle material sampler easy to use |
CN209549665U (en) * | 2019-01-17 | 2019-10-29 | 曾来珍 | A kind of biotechnology research tissue liquid separating apparatus |
Family Cites Families (1)
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EP3981868A1 (en) * | 2012-11-26 | 2022-04-13 | Fraunhofer USA, Inc. | System and method for automatic plant tissue sampling |
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2019
- 2019-12-25 CN CN201911361246.0A patent/CN111004710B/en active Active
Patent Citations (8)
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TW200708329A (en) * | 2005-08-26 | 2007-03-01 | Yen Chen Machinery Co Ltd | Extracting, concentrating, and manufacturing method of medicine solution |
AU2012207053A1 (en) * | 2006-08-31 | 2012-08-16 | Monsanto Technology Llc | Methods for producing transgenic plants |
CN102847340A (en) * | 2012-09-25 | 2013-01-02 | 湖北民族学院 | Full-automatic traditional Chinese medicinal material ingredient extracting device |
CN206828435U (en) * | 2017-03-16 | 2018-01-02 | 马健 | Sargassum protein extraction system |
CN206881716U (en) * | 2017-07-13 | 2018-01-16 | 黑龙江工业学院 | Chemical reaction kettle |
CN207175918U (en) * | 2017-07-17 | 2018-04-03 | 天津信者生物技术有限公司 | One kind rubs centrifugal plant tissue liquid extraction element |
CN207300608U (en) * | 2017-10-12 | 2018-05-01 | 山东三岳化工有限公司 | A kind of chemical reaction kettle material sampler easy to use |
CN209549665U (en) * | 2019-01-17 | 2019-10-29 | 曾来珍 | A kind of biotechnology research tissue liquid separating apparatus |
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