CN203538050U - Cleaning sterilizer for tissue culture explant of aquatic plant - Google Patents
Cleaning sterilizer for tissue culture explant of aquatic plant Download PDFInfo
- Publication number
- CN203538050U CN203538050U CN201320574431.XU CN201320574431U CN203538050U CN 203538050 U CN203538050 U CN 203538050U CN 201320574431 U CN201320574431 U CN 201320574431U CN 203538050 U CN203538050 U CN 203538050U
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- support
- collet
- ladle body
- upper cover
- explant
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Abstract
The utility model provides a cleaning sterilizer for a tissue culture explant of an aquatic plant. The cleaning sterilizer comprises a cleaning device and a sterilizing device, wherein the cleaning device comprises an upper cover, a barrel body and a support, and the sterilizing device comprises a barrel body, a collet and a support. The cleaning sterilizer is very simple and convenient to operate and capable of saving time and labor and avoiding unnecessary waste and pollution problems in the sterilizing process.
Description
Technical field:
The utility model relates to Plant Tissue Breeding vessel field, is specifically related to a kind of pasture and water group training explant washer-disinfector.
Background technology:
Plant Tissue Breeding due to its have take up room little, be not subject to the features such as area, season limit cultivation cycle be short, be applied to plant toxic, plant breeding, germ plasm resource is preserved and the aspect such as secondary metabolites extraction.Day by day perfect along with tissue culture technology, the application of its technology is also more and more wider.Pollution is recurrent problem in plant tissue culture course, because microorganism invasion makes medium and culture materials grow miscellaneous bacteria, finally causes cultivating unsuccessfully, causes unnecessary waste.From to a certain degree, can effectively solve the success or not that pollution problem has determined Plant Tissue Breeding.Prevent that microbial contamination from being the place that obtains the key of germ-free plant explant.Therefore operating personnel just seem particularly important to the cleaning of explant and disinfection.
Conventionally after using the containers such as beaker or gauze bag to hold material, with running water, rinse, easily cause flushing not thorough.The sterilization of explant material is used and be take beaker as container, add after the disinfectants such as mercuric chloride, with sterile water, repeatedly rinse, with in aseptic water washing process, the explant material that density is light swims on the aseptic water surface, tends to run off and cause unnecessary waste with toppling over waste liquid, the outside raffinate of beaker mouth refluxes, or because toppling over, dynamics is improper causes waste liquid in waste liquid cylinder to be splashed in beaker, all easily causes the pollution of material, for tissue, cultivates unsuccessfully and hides some dangers for.
Summary of the invention:
The purpose of this utility model is to provide a kind of pasture and water group training explant washer-disinfector.
Explant washer-disinfector provided by the utility model comprises cleaning device and chlorination equipment; Wherein cleaning device comprises upper cover, ladle body and support; Chlorination equipment comprises ladle body, collet and support;
Wherein the capping of upper cover is that screen cloth, the distribution of ladle body bottom even are porose, the attached one deck rubber washer of collet inwall, and a cock and long tube are arranged at collet bottom; Support has 3-4 support bar;
When wherein cleaning device uses, be that upper cover is covered on ladle body, then by ladle body frame on support; Chlorination equipment is that ladle body is placed on collet while using, and then collet is placed on support.
Wherein upper cover and ladle body form one and only hold the cylinder that flowing water passes through, and by stent support, are applicable to the flowing water rinsing step in explant cleaning process; Ladle body and collet combination, by stent support, be applicable to explant sterilization overall process, adds thimerosal or sterile water in container, after vibration sterilization or aseptic water washing, do not need to topple over, and only opens on collet and open cock, allows waste liquid flow out from collet.
Accompanying drawing explanation:
The schematic diagram of Fig. 1 upper cover
The schematic diagram of Fig. 2 ladle body
The schematic diagram of Fig. 3 collet
The schematic diagram of Fig. 4 support
The schematic diagram of Fig. 5 cleaning device
The schematic diagram of Fig. 6 chlorination equipment
Wherein: 1. upper cover; 2. ladle body; 3. collet; 4. support; 5. capping; 6. hole; 7. cock, 8 rubber washers; 9. support bar; 10. long tube
Embodiment
Below in conjunction with accompanying drawing, the utility model is done to a detailed description, but the utility model is not limited to this.
Explant washer-disinfector provided by the utility model comprises cleaning device and chlorination equipment; Wherein cleaning device comprises upper cover 1, ladle body 2 and support 4; Chlorination equipment comprises ladle body 2, collet 3 and support 4;
Wherein the capping 5 of upper cover 1 is porose 6 for screen cloth, ladle body 2 bottom evens distribute, the attached one deck rubber washer 8 of collet 3 inwall, and a cock 7 is arranged at collet 3 bottoms, and long tube 10; Support 3 has 3-4 support bar 9;
When wherein cleaning device uses, be that upper cover 1 is covered on ladle body 2, then by 2 of ladle bodies on support 4; Chlorination equipment is that the ladle body after sterilizing 2 is placed on collet 3 while using, and then collet 3 is placed on support 4.
Be preferably upper coarse and lower fine truncated cone-shaped, wherein upper surface round diameter is 8cm, and lower surface round diameter is 6cm, shaft bottom surface Circularhole diameter 1mm, the high 8-10cm of bucket, bucket wall thickness 1mm.
The diameter 6cm of support 4, thickness 5mm, below three support bars 9, the length 12cm of every support bar 9, diameter 5mm.
Explant cleaning and sterilizing container upper cover 1 of the present utility model and the optional plastics of support 4 material therefor, ladle body 2 and collet 3 are because need be through high-temperature sterilization, so select high-temperature resistance plastice or glassware.
The explant cleaning and sterilizing container that the utility model provides, upper cover 1 is screen cloth, it is porose 6 that ladle body 2 bottoms distribute, and upper cover 1 is buckled on ladle body 2, with support 4, supports, and is suitable for explant and cleans.Current flow into ladle body 2 from upper cover 1, from the hole 6 of ladle body 2 bottom surfaces, flow out.When water velocity is larger, the water in container can not flow out completely fast, can in ladle body, assemble, and the vegetable material in container is fully immersed in water, and the flowing water of realizing truly rinses; Ladle body 2 and collet 3 are combined up and down, with support 4, support, be suitable for the sterilization of explant.Close collet cock 7, then in ladle body 2, add thimerosal or sterile water, after sterilization, flushing, rotate collet cock 7, vegetable material is subject to stopping of ladle body 2 small hole at bottom parts and cannot flows out with waste liquid, waste liquid after sterilization flows out from the long tube 10 of collet 3, long tube 10 pipelines are elongated, the waste liquid having flowed out is difficult for reflecting back in container material is polluted, compare with conventional method, use this container operation very easy, time saving and energy saving, avoided waste and pollution problem unnecessary in disinfecting process simultaneously.
Claims (2)
1. a pasture and water group training explant washer-disinfector, comprises cleaning device and chlorination equipment; Wherein cleaning device comprises upper cover, ladle body and support; Chlorination equipment comprises ladle body, collet and support;
Wherein the capping of upper cover is that screen cloth, the distribution of ladle body bottom even are porose, the attached one deck rubber washer of collet inwall, and a cock and long tube are arranged at collet bottom, and support has 3-4 support bar.
2. pasture and water group training explant washer-disinfector according to claim 1, is characterized in that
When wherein cleaning device uses, be that upper cover is covered on ladle body, then by ladle body frame on support; Chlorination equipment is that ladle body is placed on collet while using, and then collet is placed on support.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201320574431.XU CN203538050U (en) | 2013-09-16 | 2013-09-16 | Cleaning sterilizer for tissue culture explant of aquatic plant |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201320574431.XU CN203538050U (en) | 2013-09-16 | 2013-09-16 | Cleaning sterilizer for tissue culture explant of aquatic plant |
Publications (1)
Publication Number | Publication Date |
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CN203538050U true CN203538050U (en) | 2014-04-16 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201320574431.XU Expired - Fee Related CN203538050U (en) | 2013-09-16 | 2013-09-16 | Cleaning sterilizer for tissue culture explant of aquatic plant |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103477986A (en) * | 2013-09-16 | 2014-01-01 | 上海市农业科学院 | Water plant tissue-culture explant cleaning and sterilizing device |
CN104026013A (en) * | 2014-06-17 | 2014-09-10 | 西北农林科技大学 | Sterile disinfection device for surfaces of plant tissues |
CN104904604A (en) * | 2015-07-10 | 2015-09-16 | 四川农业大学 | Disinfection and dewatering device for tissue culture of explants |
CN107455260A (en) * | 2017-09-13 | 2017-12-12 | 西南大学 | Xylophyta explant stem section sterilizer |
CN108668895A (en) * | 2018-04-24 | 2018-10-19 | 贵州大学 | A kind of tissue cultures explant flusher and its application method |
-
2013
- 2013-09-16 CN CN201320574431.XU patent/CN203538050U/en not_active Expired - Fee Related
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103477986A (en) * | 2013-09-16 | 2014-01-01 | 上海市农业科学院 | Water plant tissue-culture explant cleaning and sterilizing device |
CN104026013A (en) * | 2014-06-17 | 2014-09-10 | 西北农林科技大学 | Sterile disinfection device for surfaces of plant tissues |
CN104904604A (en) * | 2015-07-10 | 2015-09-16 | 四川农业大学 | Disinfection and dewatering device for tissue culture of explants |
CN107455260A (en) * | 2017-09-13 | 2017-12-12 | 西南大学 | Xylophyta explant stem section sterilizer |
CN108668895A (en) * | 2018-04-24 | 2018-10-19 | 贵州大学 | A kind of tissue cultures explant flusher and its application method |
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Legal Events
Date | Code | Title | Description |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140416 Termination date: 20150916 |
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EXPY | Termination of patent right or utility model |