CN103477986A - Water plant tissue-culture explant cleaning and sterilizing device - Google Patents

Abstract

The invention provides a water plant tissue-culture explant cleaning and sterilizing device which comprises a cleaning device and a sterilizing device. The cleaning device comprises an upper cover, a bucket body and a support. The sterilizing device comprises a bucket body, a bottom bracket and a support. The cleaning and sterilizing device is easy and convenient to operate, saves time and labor and meanwhile avoids unnecessary waste and pollution problems in the process of sterilization.

Description

A kind of pasture and water group training explant washer-disinfector

Technical field:

The present invention relates to Plant Tissue Breeding vessel field, be specifically related to a kind of pasture and water group training explant washer-disinfector.

Background technology:

Plant Tissue Breeding due to its have take up room little, be not subject to the characteristics such as area, season limit cultivation cycle be short, be applied to plant toxic, plant breeding, germ plasm resource is preserved and the aspect such as secondary metabolites extraction.Day by day perfect along with tissue culture technology, the application of its technology is also more and more wider.Pollution is recurrent problem in plant tissue culture course, because the microorganism invasion makes medium and culture materials grow miscellaneous bacteria, finally causes cultivating unsuccessfully, causes unnecessary waste.From to a certain degree, can effectively solve the success or not that pollution problem has determined Plant Tissue Breeding.Prevent that microbial contamination from being the place that obtains the key of germ-free plant explant.Therefore operating personnel just seem particularly important to cleaning and the disinfection of explant.

Usually rinse with running water after using the containers such as beaker or gauze bag to hold material, easily cause flushing not thorough.The sterilization of explant material is used and be take beaker as container, after adding the disinfectants such as mercuric chloride, with sterile water, repeatedly rinse, with in the aseptic water washing process, the explant material that density is light swims on the aseptic water surface, tends to run off and cause unnecessary waste with toppling over waste liquid, the outside raffinate of beaker mouth refluxes, or because toppling over, dynamics is improper causes waste liquid in the waste liquid cylinder to be splashed in beaker, all easily causes the pollution of material, for tissue, cultivates unsuccessfully and hides some dangers for.

Summary of the invention:

The object of the present invention is to provide a kind of pasture and water group training explant washer-disinfector.

Explant washer-disinfector provided by the present invention comprises cleaning device and chlorination equipment; Wherein cleaning device comprises upper cover, ladle body and support; Chlorination equipment comprises ladle body, collet and support;

Wherein the capping of upper cover is that screen cloth, the distribution of ladle body bottom even are porose, the attached one deck rubber washer of collet inwall, and a cock and long tube are arranged at the collet bottom; Support has 3-4 support bar;

When wherein cleaning device uses, be that upper cover is covered on ladle body, then by the ladle body frame on support; Chlorination equipment is that ladle body is placed on collet while using, and then collet is placed on support.

Wherein upper cover and ladle body form one and only hold the cylinder that flowing water passes through, and use stent support, are applicable to the flowing water rinsing step in the explant cleaning process; Ladle body and collet combination, use stent support, is applicable to explant sterilization overall process, adds thimerosal or sterile water in container, after vibration sterilization or aseptic water washing, do not need to topple over, and only opens on collet and open cock, allows waste liquid flow out and to get final product from collet.

The accompanying drawing explanation:

The schematic diagram of Fig. 1 upper cover

The schematic diagram of Fig. 2 ladle body

The schematic diagram of Fig. 3 collet

The schematic diagram of Fig. 4 support

The schematic diagram of Fig. 5 cleaning device

The schematic diagram of Fig. 6 chlorination equipment

Wherein: 1. upper cover; 2. ladle body; 3. collet; 4. support; 5. capping; 6. hole; 7. cock, 8 rubber washers; 9. support bar; 10. long tube

Embodiment

Below in conjunction with accompanying drawing, the present invention is done to a detailed description, but the present invention is not limited to this.

Explant washer-disinfector provided by the present invention comprises cleaning device and chlorination equipment; Wherein cleaning device comprises upper cover 1, ladle body 2 and support 4; Chlorination equipment comprises ladle body 2, collet 3 and support 4;

Wherein the capping 5 of upper cover 1 is porose 6 for screen cloth, ladle body 2 bottom evens distribute, the attached one deck rubber washer 8 of collet 3 inwall, and a cock 7 is arranged at collet 3 bottoms, and long tube 10; Support 3 has 3-4 support bar 9;

When wherein cleaning device uses, be that upper cover 1 is covered on ladle body 2, then by 2 of ladle bodies on support 4; Chlorination equipment is that the ladle body 2 after sterilizing is placed on collet 3 while using, and then collet 3 is placed on support 4.

Upper cover 1 is circular, and diameter is 8.5cm, and the screen cloth of capping 5 is 100 orders, the high 1cm of upper cover 1, wall thickness 1mm.

Ladle body 2 can be cylinder or upper coarse and lower fine truncated cone-shaped;

Be preferably upper coarse and lower fine truncated cone-shaped, wherein the upper surface round diameter is 8cm, and the lower surface round diameter is 6cm, shaft bottom surface Circularhole diameter 1mm, the high 8-10cm of bucket, bucket wall thickness 1mm.

Collet 3 is infundibulate, the attached one deck rubber washer 8 of inwall, and the surface circular diameter is 6.8cm, high 4cm, long tube length 6cm, diameter 1cm, thickness 1mm.

The diameter 6cm of support 4, thickness 5mm, below three support bars 9, the length 12cm of every support bar 9, diameter 5mm.

Explant cleaning and sterilizing container upper cover 1 of the present invention and the optional plastics of support 4 material therefor, ladle body 2 and collet 3 are because need be through high-temperature sterilization, so select high-temperature resistance plastice or glassware.

Explant cleaning and sterilizing container provided by the invention, upper cover 1 is screen cloth, it is porose 6 that ladle body 2 bottoms distribute, and upper cover 1 is buckled on ladle body 2, with support 4, supports, and is suitable for explant and cleans.Current flow into ladle body 2 from upper cover 1, from the hole 6 of ladle body 2 bottom surfaces, flow out.When water velocity is larger, the water in container can not flow out fully fast, can in ladle body, assemble, and the vegetable material in container is fully immersed in water, realizes that flowing water truly rinses; Ladle body 2 and collet 3 are combined up and down, support with support 4, be suitable for the sterilization of explant.Close collet cock 7, then add thimerosal or sterile water in ladle body 2, after sterilization, flushing, rotate collet cock 7, vegetable material is subject to stopping of ladle body 2 small hole at bottom parts and can't flows out with waste liquid, waste liquid after sterilization flows out from the long tube 10 of collet 3, long tube 10 pipelines are elongated, the waste liquid flowed out is difficult for reflecting back in container material is polluted, with conventional method, compare, use this container operation very easy, time saving and energy saving, avoided waste and pollution problem unnecessary in the disinfecting process simultaneously.

Claims (2)

1. a pasture and water group training explant washer-disinfector, comprise cleaning device and chlorination equipment; Wherein cleaning device comprises upper cover, ladle body and support; Chlorination equipment comprises ladle body, collet and support;

Wherein the capping of upper cover is that screen cloth, the distribution of ladle body bottom even are porose, the attached one deck rubber washer of collet inwall, and a cock and long tube are arranged at the collet bottom, and support has 3-4 support bar.

2. pasture and water group training explant washer-disinfector according to claim 1, is characterized in that

When wherein cleaning device uses, be that upper cover is covered on ladle body, then by the ladle body frame on support; Chlorination equipment is that ladle body is placed on collet while using, and then collet is placed on support.

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