CN104904604A - Disinfection and dewatering device for tissue culture of explants - Google Patents
Disinfection and dewatering device for tissue culture of explants Download PDFInfo
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- CN104904604A CN104904604A CN201510404316.1A CN201510404316A CN104904604A CN 104904604 A CN104904604 A CN 104904604A CN 201510404316 A CN201510404316 A CN 201510404316A CN 104904604 A CN104904604 A CN 104904604A
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- screen cloth
- stack shell
- explant
- sterilization
- holding rod
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Abstract
The invention discloses a disinfection and dewatering device for tissue culture of explants. The disinfection and dewatering device comprises a permeable screen mesh cylinder body, a grip rod and a screen mesh cover with the center with a hole; the permeable screen mesh cylinder body is used for accommodating the explants; the grip rod is connected with the permeable screen mesh cylinder body; the screen mesh cover is in threaded connection with the upper portion of the permeable screen mesh cylinder body; on end of the grip rod penetrates the screen mesh cover to be in threaded connection with the bottom of the permeable screen mesh cylinder body. According to the disinfection and dewatering device for the tissue culture of the explants, the structure is simple, the operation is convenient, the loss of the explants in the disinfection process can be effectively prevented, and the timely dewatering can be achieved so as to prevent browning.
Description
Technical field
The present invention relates to agricultural class field of plant tissue culture technique, specifically a kind of group training explant sterilization and dewater unit.
Background technology
Explant is the organ or tissue's fragment as cultured in vitro material in Plant Tissue Breeding, usually outdoor plant live body is taken from, a large amount of microorganism can be adhered in surface, in incubation, outer planting body surface miscellaneous bacteria is killed if do not sterilize, various miscellaneous bacteria Fast-propagation, not only seizes explant nutrient and also can produce exotoxin, affect explant growth.Therefore, before being inoculated on medium, strict sterilization must be carried out.
Explant sterilization is usually first through running water such as running water, soak with 75% alcohol short time afterwards, soak with 0.1% mercuric chloride or 10% clorox long period again after sterile water fully rinses, in immersion process, frequently rock stirring, and again use aseptic water washing 3 times.Said process is loaded down with trivial details, need repeatedly to use tweezers and prevent explant from flowing away with thimerosal or sterile water and produce loss, but effect is unsatisfactory, has loss to cause scientific research waste of material unavoidably.
Need with tweezers gripping explant (being often plant leaf blade) in aseptic water washing process, explant is often caused to stab, easily cause Brown, be unfavorable for that the later stage cultivates, and after aseptic water washing, usually explant is transferred in a container, inoculate, but due to the action need time, explant can be immersed in for a long time in the sterile water fully do not inclined before inoculation in batches, cause external body water logging undue, cause late stage of culture brownization.
Although have some about the announcement of explant surface sterilization device, if Chinese patent Authorization Notice No. is CN204191321U, CN203814333U, CN203043041U, CN203040347U, CN202232459U, CN102018969B, CN201453700U etc., but most of complex structure, parts too much fragile, manufacturing cost is high or complicated operation, be unfavorable for actual use, and all can not fully remove explant surface moisture in time, prevent brownization in explant later stage incubation.
Summary of the invention
The object of the present invention is to provide that a kind of structure is simple, the group of handled easily training explant sterilization and dewater unit.
For achieving the above object, the invention provides following technical scheme:
A kind of group training explant sterilization and dewater unit, comprise for the splendid attire explant screen cloth lid with holes with permeable screen cloth stack shell, the holding rod be connected with screen cloth stack shell and center; Described screen cloth lid is threadedly connected to the top of screen cloth stack shell; Described holding rod one end, through screen cloth lid, is threadedly connected to the bottom of screen cloth stack shell.
As the further scheme of the present invention: the bottom of described screen cloth stack shell is cone shape, and bottom apex place is provided with the connecting thread corresponding with holding rod screw thread.
As the further scheme of the present invention: the length of described holding rod is 28-40cm, one end is provided with the connecting thread corresponding with screen cloth stack shell bottom apex place screw thread.
As the further scheme of the present invention: the length of described holding rod is 30cm.
As the further scheme of the present invention: the middle part of described screen cloth lid has through hole.
As the further scheme of the present invention: described device and diameter be greater than screen cloth stack shell diameter 3-6cm, highly doubly add a cover rear height to screen cloth stack shell for 3-6 beaker with the use of.
As the further scheme of the present invention: described device and diameter be greater than screen cloth stack shell diameter 4cm, be highly 4 times of beakers of adding a cover rear height to screen cloth stack shell with the use of.
Compared with prior art, the invention has the beneficial effects as follows: structure of the present invention is simple, easy to operate, effectively can prevent the loss of explant in disinfecting process, and can dewater in time to prevent brownization.
Accompanying drawing explanation
Fig. 1 is the structural representation of group training explant sterilization and dewater unit;
Fig. 2 is the fractionation structural representation of group training explant sterilization and dewater unit;
In figure: 1-holding rod, 2-screen cloth lid, 3-screen cloth stack shell, 4-bottom apex.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Refer to Fig. 1 ~ 2, in the embodiment of the present invention, a kind of group training explant sterilization and dewater unit, comprise for the splendid attire explant screen cloth lid 2 with holes with permeable screen cloth stack shell 3, the holding rod 1 be connected with screen cloth stack shell and center; Screen cloth lid 2 is threadedly connected to the top of screen cloth stack shell 3; One end of holding rod 1, through screen cloth lid 2, is threadedly connected to the bottom of screen cloth stack shell 3; The bottom of screen cloth stack shell 3 is cone shape, and is provided with the connecting thread corresponding with screw thread on holding rod 1 at bottom apex 4 place; The length of holding rod 1 is 28-40cm, preferred 30cm, and one end of holding rod 1 is provided with the connecting thread corresponding with the bottom apex 4 place screw thread of screen cloth stack shell 3; The middle part of screen cloth lid has through hole, and its diameter, slightly larger than the external diameter of holding rod 1, makes holding rod 1 can pass from centre.
The diameter 3-6cm that the present invention need be greater than screen cloth stack shell 3 with diameter, highly doubly add a cover rear height to screen cloth stack shell 3 for 3-6 beaker with the use of.Preferably be greater than with diameter the diameter 4cm of screen cloth stack shell 3, be highly 4 times of beakers of adding a cover rear height to screen cloth stack shell 3 with the use of.
The present invention is when carrying out explant sterilization, and its course of work is as follows:
(1) first getting explant loads in screen cloth stack shell 3, threaded for holding rod 1 one end is screwed in bottom apex 4 place of screen cloth stack shell 3, be connected with screen cloth stack shell 3, then the through hole of screen cloth lid 2 through holding rod 1, be screwed on screen cloth stack shell 3, can prepare after installation to start sterilization.
(2) conveniently explant disinfection process, under holding rod 1 is tethered in water tap, running water is after 30 minutes, and whole device is placed in superclean bench.
(3) device is put into the empty beaker of 1000mL, be kept upright, the alcohol adding 75% in beaker floods to screen cloth stack shell 3, rinses after washing 30 seconds, withdrawing device, alcohol is outwelled, the sterile water of beaker volume 1/3rd is added again in beaker, after embedding device is repeatedly rinsed and is washed, withdrawing device, outwell sterile water to waste liquid cylinder, sterile water is rinsed and is washed repetition 3 times.
(4) device is put into the empty beaker of 1000mL, pouring 0.1% mercuric chloride solution into flooding screen cloth stack shell 3, soaking 8 minutes, washing and sieving net stack shell 3 rinsed by the every 2 minutes needs in midway, ensures that solution fully contacts explant; After immersion completes, withdrawing device, outwells mercuric chloride solution to waste liquid cylinder, then in beaker, adds sterile water to three/mono-volume place, embedding device, repeatedly rinses after washing, withdrawing device, outwells sterile water to waste liquid cylinder, and sterile water is rinsed and washed repetition 3 times.
(5) device is put into empty 1000mL beaker, two palms clamp the top of holding rod 1, left-hand thread direction rubbing holding rod 1, drive screen cloth stack shell 3 quick rotation, utilize centrifugal force to be thrown away by the liquid in cylinder, to dewater.
(6) after dehydration, screen cloth stack shell 3 is put in and is covered with in the culture dish of filter paper, then the filter paper getting one piece of dry sterilization encases screen barrel body 3, rotate and extract holding rod 1 out, open screen cloth lid 2, take out explant for subsequent use.
Structure of the present invention is simple, easy to operate, effectively can prevent the loss of explant in disinfecting process, and can dewater in time to prevent brownization.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
In addition, be to be understood that, although this specification is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification is only for clarity sake, those skilled in the art should by specification integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (7)
1. the sterilization of group training explant and a dewater unit, is characterized in that, comprise for the splendid attire explant screen cloth lid with holes with permeable screen cloth stack shell, the holding rod be connected with screen cloth stack shell and center; Described screen cloth lid is threadedly connected to the top of screen cloth stack shell; Described holding rod one end, through screen cloth lid, is threadedly connected to the bottom of screen cloth stack shell.
2. the sterilization of according to claim 1 group of training explant and dewater unit, it is characterized in that, the bottom of described screen cloth stack shell is cone shape, and bottom apex place is provided with the connecting thread corresponding with holding rod screw thread.
3. the sterilization of according to claim 2 group of training explant and dewater unit, it is characterized in that, the length of described holding rod is 28-40cm, and one end is provided with the connecting thread corresponding with screen cloth stack shell bottom apex place screw thread.
4. the sterilization of according to claim 3 group of training explant and dewater unit, it is characterized in that, the length of described holding rod is 30cm.
5. the sterilization of according to claim 1 group of training explant and dewater unit, it is characterized in that, the middle part of described screen cloth lid has through hole.
6. the sterilization of according to claim 1 group of training explant and dewater unit, is characterized in that, described device and diameter be greater than screen cloth stack shell diameter 3-6cm, highly be the 3-6 times beaker of adding a cover rear height to screen cloth stack shell with the use of.
7. the sterilization of according to claim 6 group of training explant and dewater unit, is characterized in that, described device and diameter be greater than screen cloth stack shell diameter 4cm, be highly 4 times of beakers of adding a cover rear height to screen cloth stack shell with the use of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510404316.1A CN104904604B (en) | 2015-07-10 | 2015-07-10 | A kind of tissue culture explant sterilization and dehydration device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510404316.1A CN104904604B (en) | 2015-07-10 | 2015-07-10 | A kind of tissue culture explant sterilization and dehydration device |
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| CN104904604A true CN104904604A (en) | 2015-09-16 |
| CN104904604B CN104904604B (en) | 2018-03-06 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935102A (en) * | 2018-08-15 | 2018-12-07 | 扬州大学 | A kind of Plant Tissue Breeding easily floats Explant sterilizing device and its sterilization method |
| CN109349112A (en) * | 2018-11-29 | 2019-02-19 | 山东省果树研究所 | A kind of explant locellus disinfection lost circulation prevention device and digital display time control speed regulation cyclotron oscillation device and its application |
| CN111226795A (en) * | 2020-03-06 | 2020-06-05 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201453700U (en) * | 2009-06-05 | 2010-05-12 | 重庆大学 | Explant surface sterilizing device |
| CN203043041U (en) * | 2013-02-07 | 2013-07-10 | 浙江诚邦园林股份有限公司 | Explant sterilizing device |
| CN203538050U (en) * | 2013-09-16 | 2014-04-16 | 上海市农业科学院 | Cleaning sterilizer for tissue culture explant of aquatic plant |
| CN203814333U (en) * | 2014-03-22 | 2014-09-10 | 福建省亚热带植物研究所 | Plant tissue culture explant immersion disinfection device |
| CN204762668U (en) * | 2015-07-10 | 2015-11-18 | 四川农业大学 | Group banks up explant disinfection and dewatering device with earth |
-
2015
- 2015-07-10 CN CN201510404316.1A patent/CN104904604B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201453700U (en) * | 2009-06-05 | 2010-05-12 | 重庆大学 | Explant surface sterilizing device |
| CN203043041U (en) * | 2013-02-07 | 2013-07-10 | 浙江诚邦园林股份有限公司 | Explant sterilizing device |
| CN203538050U (en) * | 2013-09-16 | 2014-04-16 | 上海市农业科学院 | Cleaning sterilizer for tissue culture explant of aquatic plant |
| CN203814333U (en) * | 2014-03-22 | 2014-09-10 | 福建省亚热带植物研究所 | Plant tissue culture explant immersion disinfection device |
| CN204762668U (en) * | 2015-07-10 | 2015-11-18 | 四川农业大学 | Group banks up explant disinfection and dewatering device with earth |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935102A (en) * | 2018-08-15 | 2018-12-07 | 扬州大学 | A kind of Plant Tissue Breeding easily floats Explant sterilizing device and its sterilization method |
| CN109349112A (en) * | 2018-11-29 | 2019-02-19 | 山东省果树研究所 | A kind of explant locellus disinfection lost circulation prevention device and digital display time control speed regulation cyclotron oscillation device and its application |
| CN111226795A (en) * | 2020-03-06 | 2020-06-05 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
| CN111226795B (en) * | 2020-03-06 | 2022-12-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
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| CN104904604B (en) | 2018-03-06 |
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