CN203814333U - Plant tissue culture explant immersion disinfection device - Google Patents
Plant tissue culture explant immersion disinfection device Download PDFInfo
- Publication number
- CN203814333U CN203814333U CN201420161146.XU CN201420161146U CN203814333U CN 203814333 U CN203814333 U CN 203814333U CN 201420161146 U CN201420161146 U CN 201420161146U CN 203814333 U CN203814333 U CN 203814333U
- Authority
- CN
- China
- Prior art keywords
- explant
- top cover
- cup
- tissue culture
- plant tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The utility model discloses a plant tissue culture explant immersion disinfection device and belongs to a plant tissue culture technology in the field of agriculture and forestry. The plant tissue culture explant immersion disinfection device comprises a disinfection cup containing disinfectants, an exhaust top cover with an exhaust cylinder, and a drainage top cover with a drainage screen. The disinfection cup is filled with an explant and a certain quantity of disinfectants, air in the cup is exhausted, air in an intercellular space of the explant escapes, an air hole in the exhaust top cover is opened, normal pressure in the disinfection cup is restored, the disinfectants permeate into the intercellular space of the explant, microbes existing in tissues are killed, the drainage top cover is mounted after the estimated immersion time reaches, and the explant can be prevented from flowing out along with liquid when the disinfectants are poured. The plant tissue culture explant immersion disinfection device is simple and convenient to operate, safe to plant materials and convenient to popularize and apply, and the sterilization efficiency of the disinfectants is improved.
Description
Technical field
The invention belongs to the plant tissue culture technique in agricultural field, be specifically related to explant soaking disinfection device.
Background technology
Conventional Plant Tissue Breeding explant sterilization method, that explant first rinses dozens of minutes to a few hours through flowing water, with 70% alcohol-pickled 10-30s, with aseptic water washing 2-3 time, 0.1% mercury chloride soaks 8-12min or soaks 15-20min with 2% clorox (saturated calcium hypochlorite solution).Due to the space between cells of plant tissue inside and cell interior life microorganism (endophyte), and disinfectant is difficult to infiltrate through organization internal and carries out sterilization, and endophyte is still survived, breed, causes medium to pollute, and trains to group and brings loss.Conventional solution is to add size at thimerosal, as tween-20, and Tween-80 etc., although can make disinfectant be easy to infiltrate into explantation tissue, size has compared with major injury effect plant cell equally.The difficult point that disinfectant uses is, in order effectively to kill explant attached microbial, must have higher decontaminant concentration and longer disinfecting time, and this will cause vegetable material to have compared with major injury.Although having some utility models about Explant sterilizing device announces, as application number 200920127575,201120400202,201120494069,20120107671,201220427308,20130069204,20132007042 etc., but there is not the process patent that promotes disinfectant infiltration explantation tissue.
Summary of the invention
What the utility model solved is in current Plant Tissue Breeding, because explant somatocyte gap exists air, causes thimerosal cannot infiltrate tissue endophyte is carried out the problem of sterilizing.
The purpose of this utility model is achieved through the following technical solutions:
The utility model comprises and holds the disinfectant cup of thimerosal, bleed top cover and the draining top cover with draining screen cloth with pump bowl.
Disinfectant cup is made up of high pressure resistant sterile material, and rim of a cup place establishes internal thread.
The top cover of bleeding is solid, has certain thickness, is cylinder, the external screw thread coordinating with disinfectant cup is established in bottom, side, and capping central authorities adapter sleeve plug hand pump bowl, vertically runs through the top cover of bleeding, pump bowl top is provided with handle rod, and pressure-raising can be extracted the air in disinfectant cup out repeatedly.The capping of top cover of bleeding has a ventilation hole, vertically runs through bowl cover, and ventilation hole diameter is up big and down small, and inverted round stage shape is joined inverted round stage shape rubber stopper, fits tightly, with air tight with ventilation hole.
Draining top cover lip is along fastening with sterilization rim of a cup, and there is draining screen cloth at capping edge, and dewatering screen mesh-shaped is surrounded by two opposite direction camber lines, and wherein one is the camber line at capping edge.While abandoning thimerosal, explant is all kept off in disinfectant cup by draining screen cloth.Draining top cover is made up of high pressure resistant sterile material.
Operation principle of the present utility model: explant and a certain amount of thimerosal pack disinfectant cup into, pressure-raising pump bowl handle rod pumps air in disinfectant cup repeatedly, disinfectant cup internal gas pressure is very low, and the air dissipation in explant somatocyte gap out, takes out the rubber stopper of the top cover of bleeding, allow and recover normal pressure in disinfectant cup, compressing thimerosal infiltrates explant somatocyte gap, kills the microorganism that is present in organization internal, arrives and estimates after soak time, change draining top cover, abandon thimerosal and can stop that explant flows out with liquid.
The beneficial effects of the utility model are: thimerosal more easily infiltrates space between cells, kill the microorganism that is present in explantation tissue inside, reach compared with the object of thorough disinfection explant reduction group training explant pollution rate.Because thimerosal can infiltrate space between cells soon, can reduce the medicining liquid dipping time, thereby reduce the injury of thimerosal to explantation tissue, improve explant and cultivate survival rate.
Brief description of the drawings
Fig. 1 disinfectant cup and the supporting profile of top cover of bleeding.
Fig. 2 disinfectant cup and the supporting profile of draining top cover.
Fig. 3 draining capping top view.
11 of Fig. 1---disinfectant cup, 12---the top cover of bleeding, 13---pump bowl, 14---ventilation hole, 15---rubber stopper.11 of Fig. 2---disinfectant cup, 21---draining top cover, 22---draining screen cloth.21 of Fig. 3---draining top cover, 22---draining screen cloth
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in further detail.
A kind of plant tissue culture explant soaking disinfection device, comprises disinfectant cup (11) and the top cover of bleeding (12) and the draining top cover (21) that coordinate with it respectively.
Disinfectant cup (11) and draining top cover (21) autoclaving in advance.
Explant and a certain amount of thimerosal pack disinfectant cup (11) into, disinfectant cup leaves certain space in (11), the screw-in top cover (12) of bleeding, closely cooperate with disinfectant cup (11), rubber stopper (15) is filled in ventilation hole (14), pressure-raising pump bowl (13) top handle bar repeatedly, take out the interior air of disinfectant cup (11), the rubber stopper (15) that takes out the top cover of bleeding (12) after standing a period of time makes recovery normal pressure in disinfectant cup (11).
Arrive and estimate after soak time, the back-out top cover (12) of bleeding, draining top cover (21) clinching on disinfectant cup (11), inclination disinfectant cup (11) is poured out thimerosal, the draining screen cloth (22) of draining top cover (21) can stop that explant flows out with thimerosal, after turned letter thimerosal, unplug draining top cover (21), take out explant.
Claims (2)
1. a plant tissue culture explant soaking disinfection device, is characterized in that: comprise and hold the disinfectant cup of thimerosal, bleed top cover and the draining top cover with draining screen cloth with pump bowl, disinfectant cup rim of a cup place establishes internal thread;
The top cover of bleeding is solid, is cylinder, and the external screw thread coordinating with sterilizing liquid cup is established in bottom, side, and capping central authorities adapter sleeve plug hand pump bowl, vertically runs through the bowl cover of bleeding, and can extract the air in disinfectant cup out; Draining top cover lip is along fastening with sterilization rim of a cup, and there is draining screen cloth at capping edge, abandons thimerosal and can stop that explant flows out with liquid.
2. explant soaking disinfection device as claimed in claim 1, is characterized in that: described in the top cover of bleeding have a ventilation hole, vertically run through bowl cover, ventilation hole is joined rubber stopper, with air tight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420161146.XU CN203814333U (en) | 2014-03-22 | 2014-03-22 | Plant tissue culture explant immersion disinfection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420161146.XU CN203814333U (en) | 2014-03-22 | 2014-03-22 | Plant tissue culture explant immersion disinfection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203814333U true CN203814333U (en) | 2014-09-10 |
Family
ID=51469893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420161146.XU Expired - Fee Related CN203814333U (en) | 2014-03-22 | 2014-03-22 | Plant tissue culture explant immersion disinfection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203814333U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904604A (en) * | 2015-07-10 | 2015-09-16 | 四川农业大学 | Disinfection and dewatering device for tissue culture of explants |
| CN107347640A (en) * | 2017-07-10 | 2017-11-17 | 扬州大学 | The chlorination equipment and method of a kind of plant tissue culture course |
| CN109349112A (en) * | 2018-11-29 | 2019-02-19 | 山东省果树研究所 | A kind of explant locellus disinfection lost circulation prevention device and digital display time control speed regulation cyclotron oscillation device and its application |
-
2014
- 2014-03-22 CN CN201420161146.XU patent/CN203814333U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904604A (en) * | 2015-07-10 | 2015-09-16 | 四川农业大学 | Disinfection and dewatering device for tissue culture of explants |
| CN107347640A (en) * | 2017-07-10 | 2017-11-17 | 扬州大学 | The chlorination equipment and method of a kind of plant tissue culture course |
| CN109349112A (en) * | 2018-11-29 | 2019-02-19 | 山东省果树研究所 | A kind of explant locellus disinfection lost circulation prevention device and digital display time control speed regulation cyclotron oscillation device and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| GB2515406A (en) | Apparatus and method for reducing populations of mosquitoes and the like | |
| CN203814333U (en) | Plant tissue culture explant immersion disinfection device | |
| CN204888280U (en) | Plant tissue culture explant degassing unit | |
| CN104904604B (en) | A kind of tissue culture explant sterilization and dehydration device | |
| CN210432380U (en) | Sterilization device is used in processing of pumpkin seed | |
| CN102940300A (en) | Fresh-keeping method for penaeus vannamei | |
| CN204860223U (en) | Kiwi fruit shoot root system disinfection groove | |
| CN204191321U (en) | A kind of fountain Explant sterilizing device | |
| CN204362540U (en) | Paddy rice interval is soaked seed box | |
| CN204394383U (en) | A kind of Cleaning tool for mouth bactericidal cabinet | |
| CN206165518U (en) | Automatic moisturizing fish bowl | |
| CN205850568U (en) | After utilizing atomization, high-concentration ozone water carries out cultivating the device of industry space taste removal | |
| CN207313201U (en) | A kind of preparation facilities of nanometer of bubble functional ionized water | |
| CN201643016U (en) | Double-cover sterilization drum | |
| CN203922821U (en) | A kind of osmosis type chlorine dioxide adding device | |
| CN212138303U (en) | Ecological agriculture seed environmental protection sterilization drying device | |
| CN203618312U (en) | Special disinfecting and sterilizing screen box for sterile culture of orchid seeds | |
| CN202490209U (en) | Novel thermometer sterilizer case | |
| CN204814952U (en) | Plant's vehicle gets into disinfection device | |
| CN202207690U (en) | Cleaning basket for oxygen humidifying bottle | |
| CN203896956U (en) | Device for cleaning and disinfecting seeds | |
| CN208545152U (en) | A kind of sterilizing unit of portable reverse osmosis fungicide | |
| CN103622137A (en) | Ozone sterilization device for fermenting tank | |
| CN105558754A (en) | Green purifying and residue removing system for nuts and application of system | |
| CN202774028U (en) | Ozone sterilization device for fermenting tank |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140910 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |