CN108935102A - A kind of Plant Tissue Breeding easily floats Explant sterilizing device and its sterilization method - Google Patents
A kind of Plant Tissue Breeding easily floats Explant sterilizing device and its sterilization method Download PDFInfo
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- CN108935102A CN108935102A CN201810927735.7A CN201810927735A CN108935102A CN 108935102 A CN108935102 A CN 108935102A CN 201810927735 A CN201810927735 A CN 201810927735A CN 108935102 A CN108935102 A CN 108935102A
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- beaker
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
Easily float Explant sterilizing device and its sterilization method, including screen barrel, seal closure the present invention relates to a kind of Plant Tissue Breeding, screen barrel is equipped with several sieve pores, screen barrel it is open at one end, seal closure is placed on the opening of screen barrel；The opening side of the screen barrel is equipped with connection card, and seal closure is hingedly coupled in connection card, and adjustable handle is fixed in connection card, and adjustable handle is vertical with the opening of screen barrel；The adjustable handle is equipped with sliding slot, is equipped with adjustable button in sliding slot, and adjustable button slides up and down along the chute；It is additionally provided with connection handle, one end of connection handle is fixedly connected with adjustable button, and the other end is placed in seal closure top, and one end for being fixedly connected with adjustable button of connection handle is higher than the other end of connection handle.Through the invention, guarantee that explant completely attaches to thimerosal, can be sufficiently dehydrated again after disinfection, small explant dehydration difficult problem is avoided, to guarantee the integrality of explant and improve explant disinfection efficiency.
Description
Technical field
The present invention relates to a kind of Plant Tissue Breeding easily to float Explant sterilizing device and its sterilization method, belongs to plant
Object field of tissue culture, disinfection and dehydration suitable for explant disinfection dehydration, microminiature and easily floating explant.
Background technique
Plant Tissue Breeding is a special kind of skill developed based on cellular omnipotency, and primary operational process is exactly will
After the explants such as plant organ tissue or cell are separated from donor plant, by disinfection, it is inoculated on suitable culture medium,
Pass through dedifferentiation under the aseptic condition of artificial creation and breaking up, develops into intact plant.Made in its explant disinfecting process
Explant mainly includes root, stem, leaf, flower, bud and fruit and seed of plant etc..
Either from nature or the explant of indoor acquisition, micro- lifes such as all kinds of fungi bacteriums and virus are all had
Object, if will result in the pollution of culture materials during tissue culture without disinfection treatment and influence the normal growth of group training material,
It therefore is the successful basic premise of tissue cultures and guarantee to the disinfection treatment of explant.It is needed in disinfecting process thorough in disinfection
Under the premise of guarantee explant intact degree and avoid polluting again.Many explants easily float in disinfecting process, hold
The halfway situation of disinfection is easily caused, microminiature explant is sterilized, easily occur that dehydration is inconvenient to cause to pollute again can
Energy.
Summary of the invention
The purpose of the present invention is to above-mentioned problem, a kind of Plant Tissue Breeding is provided easily floats explant and disappear
Malicious device and its sterilization method, are applied to explant disinfecting process, and simple and convenient operating method can be opened with improving disinfection efficiency
Screen barrel is closed to reduce damage of the explant in disinfecting process.
The object of the present invention is achieved like this, and a kind of Plant Tissue Breeding easily floats Explant sterilizing device, feature
Be, including screen barrel, seal closure, screen barrel be equipped with several sieve pores, screen barrel it is open at one end, seal closure is placed on screen barrel
Opening；The opening side of the screen barrel is equipped with connection card, and seal closure is hingedly coupled in connection card, and fixed in connection card
There is adjustable handle, adjustable handle is vertical with the opening of screen barrel；
The adjustable handle is equipped with sliding slot, is equipped with adjustable button in sliding slot, and adjustable button slides up and down along the chute；
It is additionally provided with connection handle, one end of connection handle is fixedly connected with adjustable button, and the other end is placed in seal closure top, and connection handle
The one end being fixedly connected with adjustable button is higher than the other end of connection handle；Adjustable button slides up and down along the chute drives connection handle not
Extruding of one end extruding sealing lid or release being fixedly connected with adjustable button to seal closure；When connection handle is solid not with adjustable button
When one end extruding sealing lid connected surely, seal closure is tightly placed on the opening of screen barrel；When connection handle is not fixed with adjustable button
When one end release of connection squeezes seal closure, seal closure can be opened from the opening of screen barrel.
The seal closure is equipped with annular recess towards the one side that screen barrel is open, and seal closure is placed on the opening of screen barrel
When, barrel is placed in annular recess at the top of the screen barrel of screen barrel opening so that seal closure formed with screen barrel mouth it is closed,
Prevent explant from flowing out.
Several permeable holes are distributed on the seal closure, the size of permeable hole is 40 mesh, to keep water flow clear and coherent and prevent
Only explant flows out.
The sliding slot runs through the adjustable handle other side from adjustable handle side.
Nut is screwed on the adjustable button, and nut is placed in outside the sliding slot on adjustable handle, nut is along adjustable
When the direction of button towards adjustable handle screws, nut is close to adjustable handle, and adjustable button is fixed on the cunning on adjustable handle
In slot；When nut is screwed along the direction that button is adjusted towards adjustable handle backwards, nut is not bonded with adjustable handle, adjustable
Section button can be slided up and down along the sliding slot on adjustable handle.
Sieve pore is less than the size of explant on the screen barrel.
A kind of Plant Tissue Breeding easily floats Explant sterilizing device and carries out explant sterilization method, which is characterized in that packet
Include following steps:
Step 1) will need the explant sterilized to be fitted into chlorination equipment, first by the adjustable button on adjustable handle adjustable
The highest point for being adjusted to sliding slot in the sliding slot of handle is saved, seal closure is opened, the explant sterilized will be needed to put from the opening of screen barrel
Enter in screen barrel；It is then shut off seal closure, adjustable knob is adjusted to the sliding slot lowest point of adjustable handle, and adjustable knob is fixed
In the sliding slot lowest point of adjustable handle, one end extruding sealing lid that connection handle is not fixedly connected with adjustable button, sealing is covered tightly
It is tightly placed on the opening of screen barrel, so that seal closure is combined closely with screen barrel, prevents the explant in screen barrel from flowing out；It completes to need
The explant of disinfection is fitted into chlorination equipment；
Step 2 places the first beaker, the second beaker, third beaker, the 4th beaker, the 5th beaker, on superclean bench
Six beakers, the alcohol that 70-75% is poured into the first beaker is stand-by, and it is stand-by to pour into sterile water in the second beaker, in third beaker
It is stand-by inside to pour into 1% liquor natrii hypochloritis, it is stand-by to pour into sterile water in the 4th beaker, the 5th beaker, the 6th beaker；It will be through
After the chlorination equipment that step 1) places disinfection explant in need is placed under flowing water and rinses 1-2 hours, chlorination equipment is placed in super
It is stand-by on net workbench；
Chlorination equipment is placed into superclean bench by step 3), and chlorination equipment is put in the first beaker on superclean bench,
In the alcohol that chlorination equipment is submerged in 70-75% in the first beaker, stirring is taken out after impregnating 10-20s, is stirred in chlorination equipment
In soaking process, alcohol enters in screen barrel from several sieve pores on screen barrel, so that the explant in screen barrel is in alcohol
Stirring is impregnated；
Chlorination equipment is transferred in the second beaker again, stirs and impregnates after being flooded in the sterile water in the second beaker, is filled in disinfection
It sets in stirring soaking process, the sterile water in the second beaker enters in screen barrel from several sieve pores on screen barrel, so that sieve
Explant in cylinder stirs immersion in the sterile water in the second beaker；
Then, chlorination equipment is transferred in third beaker, leaching is stirred after flooding in 1% liquor natrii hypochloritis in third beaker
It is taken out after bubble 10-20min, in chlorination equipment stirring soaking process, 1% liquor natrii hypochloritis in third beaker is from screen barrel
On several sieve pores enter in screen barrel so that the explant in screen barrel stirs in 1% liquor natrii hypochloritis in third beaker
Mix immersion；
The sterile water logging impregnated, in the 5th beaker is stirred after chlorination equipment to be successively transferred to the sterile water submerged in the 4th beaker again
Stirring impregnates, stirs and impregnate after the sterile water submerged in the 6th beaker after not, and chlorination equipment is in the 4th beaker, the 5th beaker, the
When in six beakers, the 4th beaker, the 5th beaker, the sterile water in the 6th beaker enter sieve from several sieve pores on screen barrel
In cylinder, so that the explant successively stirring immersion, the 5th beaker after the sterile water submerged in the 4th beaker respectively in screen barrel
Stirring impregnates, stirs and impregnate after the sterile water submerged in the 6th beaker after interior sterile water submerged, so that chlorination equipment uses nothing
Bacterium water washing is clean；
Step 4), will through step 5), treated dries chlorination equipment surface with aseptic filter paper after chlorination equipment drains away the water, so
Adjustable button is adjusted to the highest point of sliding slot in the sliding slot of adjustable handle afterwards, seal closure is opened, is taken explant with tweezers
Out into the culture dish for filling aseptic filter paper；
Step 5), the explant in the culture dish of aseptic filter paper after suck dry moisture, after being sterilized.
Structure of the invention is reasonable, it is easy, easy to use to manufacture, and through the invention, specific technical solution of the present invention is:
A kind of Plant Tissue Breeding easily floats Explant sterilizing device, including screen barrel, seal closure, if being provided with dry screen on screen barrel
Hole, screen barrel it is open at one end, seal closure is placed on the opening of screen barrel；In the opening side of screen barrel, connection card, sealing are set
Cover hinge is connected in connection card, and adjustable handle is fixed in connection card, and adjustable handle is vertical with the opening of screen barrel；
Sliding slot is set on adjustable handle, adjustable button is installed in sliding slot, adjustable button slides up and down along the chute；The one of connection handle
End is fixedly connected with adjustable button, and the other end is placed in seal closure top, and one end that connection handle is fixedly connected with adjustable button is high
In the other end of connection handle.
When in use, adjustable button slides up and down the one end for driving connection handle not to be fixedly connected with adjustable button along the chute and squeezes
The extruding of press seal lid or release to seal closure；When one end extruding sealing lid that connection handle is not fixedly connected with adjustable button,
Seal closure is tightly placed on the opening of screen barrel；When one end release that connection handle is not fixedly connected with adjustable button squeezes seal closure
When, seal closure can be opened from the opening of screen barrel.
The material of whole device of the present invention is stainless steel, is hardly damaged in disinfecting process.Further, the sealing
It is equipped with groove below lid, can combine closely with screen barrel.Further, the sieve screen apertures size of screen barrel is big according to explant
The screen barrel of small optional different meshes carries out explant disinfection, to guarantee highest disinfection efficiency.Seal closure, adjustable handle
And screen barrel is connected by connection card.
Compared with prior art, a kind of Plant Tissue Breeding of the present invention easily float Explant sterilizing device have with
Lower advantage:
Structure is simple, easy to operate, when one end extruding sealing lid not being fixedly connected with adjustable button by connection handle, seal closure
It is tightly placed on the opening of screen barrel, screen barrel can be made to form confined space, the explant outflow for preventing microminiature and easily floating is protected
It demonstrate,proves explant and completely attaches to thimerosal, can be sufficiently dehydrated again after disinfection, avoid small explant dehydration difficult problem, thus
Guarantee the integrality of explant and improves explant disinfection efficiency.
Detailed description of the invention
Fig. 1 is overall structure diagram of the invention.
Fig. 2 is the overlook direction structural schematic diagram of screen barrel in the present invention.
Fig. 3 is that seal closure looks up direction structure schematic diagram in the present invention.
Fig. 4 is the structural schematic diagram of adjustable handle in the present invention.
Fig. 5 is screen barrel structural schematic diagram in the present invention.
Fig. 6 is seal closure overlook direction structural schematic diagram in the present invention.
In figure: 1 adjustable button, 2 adjustable handles, 3 connection cards, 4 connection handles, 5 seal closures, 6 screen barrels, 7 sieve pores, 8 are thoroughly
Water hole.
Specific embodiment
Below in conjunction with attached drawing and Detailed description of the invention, the present invention is further illustrated.
A kind of Plant Tissue Breeding easily floats Explant sterilizing device, including screen barrel 6, seal closure 5, on screen barrel 6
Several sieve pores 7 are set, screen barrel 6 it is open at one end, seal closure 5 is placed on the opening of screen barrel 6；The opening side of screen barrel 6 is set
Connection card 3 is set, seal closure 5 is hingedly coupled in connection card 3, and adjustable handle 2 is fixed in connection card 3, adjustable handle 2
It is vertical with the opening of screen barrel 6.
Sliding slot is set on adjustable handle 2, adjustable button 1 is installed in sliding slot, the upper downslide along the chute of button 1 is adjusted
It is dynamic；It is additionally provided with connection handle 4, one end of connection handle 4 is fixedly connected with adjustable button 1, and the other end is placed in 5 top of seal closure, and
One end that connection handle 4 is fixedly connected with adjustable button 1 is higher than the other end of connection handle 4；Adjustable button 1 slides up and down band along the chute
Extruding of one end extruding sealing lid 5 or release that dynamic connection handle 4 is not fixedly connected with adjustable button 1 to seal closure 5；Work as connection handle
4 be not fixedly connected with adjustable button 1 one end extruding sealing lid 5 when, seal closure 5 is tightly placed on the opening of screen barrel 6；Work as connection
When one end release that handle 4 is not fixedly connected with adjustable button 1 squeezes seal closure 5, seal closure 5 can be beaten from the opening of screen barrel 6
It opens.
Annular recess is set in the one side that seal closure 5 is open towards screen barrel 6, and seal closure 5 is placed on the opening of screen barrel 6
When, the screen barrel top barrel of 6 opening of screen barrel is placed in annular recess, so that seal closure 5 and 6 mouthfuls of formation of screen barrel are close
It closes, prevents explant from flowing out.Several permeable holes 8 are distributed on seal closure 5, the size of permeable hole is 40 mesh, to keep water
It circulates suitable and prevents explant from flowing out.Sliding slot runs through 2 other side of adjustable handle from 2 side of adjustable handle.
Nut is screwed on adjustable button 1, and nut is placed in outside the sliding slot on adjustable handle 2, nut is along adjustable
When the direction of button 1 towards adjustable handle 2 screws, nut is close to adjustable handle 2, and adjustable button 1 is fixed on adjustable handle 2
Sliding slot in；When nut is screwed along the direction that button 1 is adjusted towards adjustable handle 2 backwards, nut is not pasted with adjustable handle 2
It closes, adjustable button 1 can be slided up and down along the sliding slot on adjustable handle 2.
When easily floating Explant sterilizing device progress explant disinfection using a kind of Plant Tissue Breeding, including following step
It is rapid:
Step 1) will need the explant that sterilizes to be fitted into chlorination equipment, first by the adjustable button 1 on adjustable handle 2 can
It is adjusted to the highest point of sliding slot in the sliding slot of regulation handle 2, opens seal closure 5, explant the opening from screen barrel 6 that needs are sterilized
Mouth is put into screen barrel 6；It is then shut off seal closure 5, adjustable knob 1 is adjusted to the sliding slot lowest point of adjustable handle 2, and adjustable
Section knob 1 is fixed on the sliding slot lowest point of adjustable handle 2, one end extruding sealing that connection handle 4 is not fixedly connected with adjustable button 1
Lid 5, seal closure 5 are tightly placed on the opening of screen barrel 6, and seal closure 5 is made to combine closely with screen barrel 6, and sieve pore 7 is small on screen barrel 6
In the size of explant, prevent the explant in screen barrel 6 from flowing out；It completes that the explant sterilized is needed to be fitted into chlorination equipment；
Step 2 places the first beaker, the second beaker, third beaker, the 4th beaker, the 5th beaker, on superclean bench
Six beakers, the alcohol that 70-75% is poured into the first beaker is stand-by, and it is stand-by to pour into sterile water in the second beaker, in third beaker
It is stand-by inside to pour into 1% liquor natrii hypochloritis, it is stand-by to pour into sterile water in the 4th beaker, the 5th beaker, the 6th beaker；It will be through
After the chlorination equipment that step 1) places disinfection explant in need is placed under flowing water and rinses 1-2 hours, chlorination equipment is placed in super
It is stand-by on net workbench；
Chlorination equipment is placed into superclean bench by step 3), and chlorination equipment is put in the first beaker on superclean bench,
In the alcohol that chlorination equipment is submerged in 70-75% in the first beaker, stirring is taken out after impregnating 10-20s, is stirred in chlorination equipment
In soaking process, alcohol enters in screen barrel 6 from several sieve pores 7 on screen barrel 6, so that the explant in screen barrel 6 is in wine
It stirs and impregnates in essence；
Chlorination equipment is transferred in the second beaker again, stirs and impregnates after being flooded in the sterile water in the second beaker, is filled in disinfection
It sets in stirring soaking process, the sterile water in the second beaker enters in screen barrel 6 from several sieve pores 7 on screen barrel 6, so that sieve
Explant in net cylinder 6 stirs immersion in the sterile water in the second beaker；
Then, chlorination equipment is transferred in third beaker, leaching is stirred after flooding in 1% liquor natrii hypochloritis in third beaker
It is taken out after bubble 10-20min, in chlorination equipment stirring soaking process, 1% liquor natrii hypochloritis in third beaker is from screen barrel 6
On several sieve pores 7 enter screen barrel 6 in so that 1% liquor natrii hypochloritis of the explant in screen barrel 6 in third beaker
Middle stirring is impregnated；
The sterile water logging impregnated, in the 5th beaker is stirred after chlorination equipment to be successively transferred to the sterile water submerged in the 4th beaker again
Stirring impregnates, stirs and impregnate after the sterile water submerged in the 6th beaker after not, and chlorination equipment is in the 4th beaker, the 5th beaker, the
When in six beakers, the 4th beaker, the 5th beaker, the sterile water in the 6th beaker enter sieve from several sieve pores 7 on screen barrel 6
In net cylinder 6, so that the explant successively stirring immersion, the 5th after the sterile water submerged in the 4th beaker respectively in screen barrel 6
Stirring impregnates, stirs and impregnate after the sterile water submerged in the 6th beaker after sterile water submerged in beaker, so that chlorination equipment makes
With sterile water washes clean；
Step 4), will through step 5), treated dries chlorination equipment surface with aseptic filter paper after chlorination equipment drains away the water, so
Adjustable button 1 is adjusted to the highest point of sliding slot in the sliding slot of adjustable handle 2 afterwards, opens seal closure 5, with tweezers by explant
It takes out into the culture dish for filling aseptic filter paper；
Step 5), the explant in the culture dish of aseptic filter paper after suck dry moisture, after being sterilized.Due to chlorination equipment volume
It is small, it can reach the multiple effect of once sterilizing, in the beaker of 1000ml to improve disinfection efficiency.
Further, since chlorination equipment is substantially carried out the disinfection of microminiature explant, chlorination equipment size is also smaller, when disinfection
Multiple chlorination equipments equipped with explant can be put into simultaneously in large beaker to carry out disinfection, disinfection efficiency is improved, to reach one
The multiple effect of secondary disinfection.
The configuration of the present invention is simple, it is easy to operate, the loss of explant in disinfecting process can be prevented, and overcome explant de-
The problems such as water is difficult.
Claims (7)
1. a kind of Plant Tissue Breeding easily floats Explant sterilizing device, which is characterized in that including screen barrel (6), seal closure
(5), screen barrel (6) be equipped with several sieve pores (7), screen barrel (6) it is open at one end, seal closure (5) is placed on opening for screen barrel (6)
Mouthful；The opening side of the screen barrel (6) is equipped with connection card (3), and seal closure (5) is hingedly coupled on connection card (3), and connects
It is fixed with adjustable handle (2) on card (3), adjustable handle (2) is vertical with the opening of screen barrel (6)；
The adjustable handle (2) is equipped with sliding slot, is equipped with adjustable button (1) in sliding slot, and button (1) is adjusted along the chute up and down
Sliding；
It is additionally provided with connection handle (4), one end of connection handle (4) is fixedly connected with adjustable button (1), and the other end is placed in seal closure (5)
Top, and one end for being fixedly connected with adjustable button (1) of connection handle (4) is higher than the other end of connection handle (4)；Adjustable button (1)
One end extruding sealing lid (5) for driving connection handle (4) not to be fixedly connected with adjustable button (1) or release pair are slided up and down along the chute
The extruding of seal closure (5)；When one end extruding sealing lid (5) that connection handle (4) is not fixedly connected with adjustable button (1), sealing
Lid (5) is tightly placed on the opening of screen barrel (6)；When one end that connection handle (4) is not fixedly connected with adjustable button (1) is discharged to envelope
When lid (5) squeezes, seal closure (5) can be opened from the opening of screen barrel (6).
2. a kind of Plant Tissue Breeding according to claim 1 easily floats Explant sterilizing device, which is characterized in that described
Seal closure (5) is equipped with annular recess towards the one side that screen barrel (6) is open, and seal closure (5) is placed on the opening of screen barrel (6)
When, the screen barrel top barrel of screen barrel (6) opening is placed in annular recess, so that seal closure (5) and screen barrel (6) mouth
It is formed closed, prevents explant from flowing out.
3. a kind of Plant Tissue Breeding according to claim 1 easily floats Explant sterilizing device, which is characterized in that described
It is distributed on seal closure (5) several permeable holes (8), the size of permeable hole is 40 mesh, to keep water flow clear and coherent and prevent explant
Body outflow.
4. a kind of Plant Tissue Breeding according to claim 1 easily floats Explant sterilizing device, which is characterized in that described
Sliding slot runs through adjustable handle (2) other side from adjustable handle (2) side.
5. a kind of Plant Tissue Breeding according to claim 1 easily floats Explant sterilizing device, which is characterized in that described
It is screwed with nut on adjustable button (1), and nut is placed in outside the sliding slot on adjustable handle (2), nut is along adjustable button (1) court
When the direction of adjustable handle (2) screws, nut is close to adjustable handle (2), and adjustable button (1) is fixed on adjustable handle
(2) in the sliding slot on；When nut is along being adjusted button (1) towards when screwing backwards to the direction of adjustable handle (2), nut not with it is adjustable
Handle (2) fitting is saved, adjustable button (1) can be slided up and down along the sliding slot on adjustable handle (2).
6. a kind of Plant Tissue Breeding according to claim 1 easily floats Explant sterilizing device, which is characterized in that described
Sieve pore (7) is less than the size of explant on screen barrel (6).
7. a kind of Plant Tissue Breeding described in -6 any one claims easily floats explant disinfection dress according to claim 1
It sets and carries out explant sterilization method, which comprises the following steps:
Step 1) will need the explant sterilized to be fitted into chlorination equipment, first by the adjustable button (1) on adjustable handle (2)
It is adjusted to the highest point of sliding slot in the sliding slot of adjustable handle (2), opens seal closure (5), by the explant for needing to sterilize from sieve
The opening of net cylinder (6) is put into screen barrel (6)；Seal closure (5) are then shut off, adjustable knob (1) is adjusted to adjustable handle (2)
Sliding slot lowest point, and adjustable knob (1) is fixed on the sliding slot lowest point of adjustable handle (2), connection handle (4) not with it is adjustable
One end extruding sealing lid (5) that button (1) is fixedly connected, seal closure (5) are tightly placed on the opening of screen barrel (6), make seal closure (5)
It combines closely with screen barrel (6), prevents the explant in screen barrel (6) from flowing out；It completes the explant for needing to sterilize and is packed into disinfection
In device；
Step 2 places the first beaker, the second beaker, third beaker, the 4th beaker, the 5th beaker, on superclean bench
Six beakers, the alcohol that 70-75% is poured into the first beaker is stand-by, and it is stand-by to pour into sterile water in the second beaker, in third beaker
It is stand-by inside to pour into 1% liquor natrii hypochloritis, it is stand-by to pour into sterile water in the 4th beaker, the 5th beaker, the 6th beaker；It will be through
After the chlorination equipment that step 1) places disinfection explant in need is placed under flowing water and rinses 1-2 hours, chlorination equipment is placed in super
It is stand-by on net workbench；
Chlorination equipment is put in the first beaker by step 3) on superclean bench, and chlorination equipment is submerged in the first beaker
In the alcohol of 70-75%, stirring is taken out after impregnating 10-20s, and in chlorination equipment stirring soaking process, alcohol is from screen barrel (6)
On several sieve pores (7) enter screen barrel (6) in so that the explant in screen barrel (6) stirs immersion in alcohol；
Chlorination equipment is transferred in the second beaker again, stirs and impregnates after being flooded in the sterile water in the second beaker, is filled in disinfection
It sets in stirring soaking process, the sterile water in the second beaker enters in screen barrel (6) from several sieve pores (7) on screen barrel (6),
So that the explant in screen barrel (6) stirs immersion in the sterile water in the second beaker；
Then, chlorination equipment is transferred in third beaker, leaching is stirred after flooding in 1% liquor natrii hypochloritis in third beaker
It is taken out after bubble 10-20min, in chlorination equipment stirring soaking process, 1% liquor natrii hypochloritis in third beaker is from screen barrel
(6) several sieve pores (7) on enter in screen barrel (6), so that 1% chlorine of the explant in screen barrel (6) in third beaker
It stirs and impregnates in acid sodium solution；
The sterile water logging impregnated, in the 5th beaker is stirred after chlorination equipment to be successively transferred to the sterile water submerged in the 4th beaker again
Stirring impregnates, stirs and impregnate after the sterile water submerged in the 6th beaker after not, and chlorination equipment is in the 4th beaker, the 5th beaker, the
When in six beakers, the 4th beaker, the 5th beaker, the sterile water in the 6th beaker from several sieve pores (7) on screen barrel (6) into
Enter in screen barrel (6), so that the explant in screen barrel (6) successively stirs leaching after the sterile water submerged in the 4th beaker respectively
Stirring impregnates, stirs and impregnate after the sterile water submerged in the 6th beaker after sterile water submerged in bubble, the 5th beaker, so that disinfection
Device uses sterile water washes clean；
Step 4), will through step 5), treated dries chlorination equipment surface with aseptic filter paper after chlorination equipment drains away the water, so
Adjustable button (1) is adjusted to the highest point of sliding slot in the sliding slot of adjustable handle (2) afterwards, is opened seal closure (5), it will with tweezers
Explant takes out into the culture dish for filling aseptic filter paper；
Step 5), the explant in the culture dish of aseptic filter paper after suck dry moisture, after being sterilized.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810927735.7A CN108935102B (en) | 2018-08-15 | 2018-08-15 | Plant tissue culture and easy-floating explant disinfection device and disinfection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810927735.7A CN108935102B (en) | 2018-08-15 | 2018-08-15 | Plant tissue culture and easy-floating explant disinfection device and disinfection method thereof |
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| CN108935102A true CN108935102A (en) | 2018-12-07 |
| CN108935102B CN108935102B (en) | 2022-03-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109349112A (en) * | 2018-11-29 | 2019-02-19 | 山东省果树研究所 | A kind of explant locellus disinfection lost circulation prevention device and digital display time control speed regulation cyclotron oscillation device and its application |
| CN111226795A (en) * | 2020-03-06 | 2020-06-05 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
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| CN111226795B (en) * | 2020-03-06 | 2022-12-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
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