CN110249926A - A kind of method of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling - Google Patents

A kind of method of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling Download PDF

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Publication number
CN110249926A
CN110249926A CN201910708519.8A CN201910708519A CN110249926A CN 110249926 A CN110249926 A CN 110249926A CN 201910708519 A CN201910708519 A CN 201910708519A CN 110249926 A CN110249926 A CN 110249926A
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China
Prior art keywords
baoshan
madder
sphagna
seed
seedling
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CN201910708519.8A
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Chinese (zh)
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CN110249926B (en
Inventor
耿云芬
李静
余丽芝
杨蔷
张劲峰
杨昌吉
耿绍东
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Longyang Branch Of Mountain Preservation And Conservation Bureau Gaoligongshan National Nature Reserve Yunnan Province
Yunnan Academy of Forestry
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Longyang Branch Of Mountain Preservation And Conservation Bureau Gaoligongshan National Nature Reserve Yunnan Province
Yunnan Academy of Forestry
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Priority to CN201910708519.8A priority Critical patent/CN110249926B/en
Publication of CN110249926A publication Critical patent/CN110249926A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • A01G9/0299Handling or transporting of soil blocks or seedlings

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The present invention provides a kind of methods of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling, comprising the following steps: (1) acquisition of fruit and seed: acquisition exocarp is the Baoshan madder fruit of celadon;Exocarp is removed;Taking out kind of skin is light brown or dark brown leopard grain pattern film quality, endosperm slightly sclerotin or sclerotin Baoshan madder seed;(2) seed disinfection;(3) sphagna vernalization: fresh seeds are layered on sphagna, repave one layer of sphagna by one layer of sphagna of paving;(4) preparation of transplanting medium;(5) it transplants: directly supporting root in sphagna, when radicle is long to have root hair, radicle axis green to 3 ± 0.3cm, radicle, in transplanting to container for plant growth, the root with root hair is planted in seedling medium, the thick stock cream of radicle axis and package cotyledon exposes outside seedling medium, pours 800 times of liquid of carbendazim or Bravo;(6) it manages.The germination rate and planting percent of Baoshan madder seed is greatly improved in the present invention, shortens seedling raise period, nursery stock germination is neat, manageability.

Description

A kind of method of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling
Technical field
The present invention relates to field of plant reproduction, and in particular to a kind of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling Method.
Background technique
Mountain madder (Fosbergia shweliensis) is madder University of Science and Technology fruit madder platymiscium, the endemic species of gaoligong mountains. It is rediscovered again after disappearing 85 years, population distribution in field is few, and population is small, and existing plant quantity is few, belongs to minimum kind Group's species.For minimum population species because of its population quantity rareness, distribution is narrow, is in endangered state, is compromised object The monoid of protection is most needed in kind.
The research discovery Baoshan madder result time needs for many years by inquiry, and fruit is harbored, hung on the tree when 5-6 Between, new fruit and ripening fruits differentiate difficulty;Fruit exocarp thickness, lignifying, not perishable and opening;Seed is by thick and bone Endosperm coating of change etc., therefore Baoshan madder result time, the harboring of fruit, the judgement of fruit maturation feature, fruit and seed The reasons such as structure are to cause Baoshan madder seminal propagation difficulty, without one of natural regeneration, species reason in imminent danger.The Baoshan is alizarin at present Seed seedling-raising technology have not been reported.
Summary of the invention
The invention proposes a kind of method of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling, the artificial breeding sides The germination rate and planting percent of Baoshan madder seed is greatly improved in method, shortens seedling raise period, is that the rare or endangered species Baoshan is alizarin Quick breeding, conservation biology restore ecological and resource development and utilization etc. the effective technological means of offer.
In order to achieve the above object, the present invention is achieved by the following technical programs:
A kind of method of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling, comprising the following steps:
(1) acquisition of fruit and seed: acquisition exocarp is the Baoshan madder fruit of celadon;With sharp knife by exocarp Removing;Taking out kind of skin is light brown or dark brown leopard grain pattern film quality, endosperm slightly sclerotin or sclerotin Baoshan madder seed;
(2) seed disinfection;
(3) sphagna vernalization: solid carbon dioxide tongue fur being placed in water and is impregnated with, spare after can not holding moisture with hand after pulling out;
One sealable container of preparation or sealable polybag, the layer overlay sphagna in container or polybag, then The fresh Baoshan madder seed for sterilizing wash clean is layered on sphagna, then then at one layer of the seed upper berth completed sphagna, then is sealed Container or polybag;
(4) preparation of transplanting medium: in being put into seedling medium in container for plant growth, seedling medium is the mixed of loam and humus Matrix is closed, transplanting the previous day sprinkles profoundly water seedling medium;
(5) it transplants: directly supporting root in sphagna and moved when radicle is long to have root hair, radicle axis green to 3 ± 0.3cm, radicle It plants to container for plant growth, wherein the root with root hair is planted in ventilative seedling medium, the thick stock cream dew of radicle axis and package cotyledon Out outside seedling medium, 800 times of liquid of 50% carbendazim, 800 times of liquid or 75% Bravo are then poured;
(6) it manages: keeping ground moistening, 800 times of liquid of 50% carbendazim or 75% Bravo 800 were sprayed every 7 days Times liquid, emerges after 20 days.
Preferably, in step (2), seed disinfection specifically: the fresh seeds of taking-up are cleaned up with clear water, then use matter The potassium permanganate that amount concentration is 0.1% rinses spare after impregnating 30 minutes.
It preferably,, can be to container or plastics to avoid seed bored bad when environment temperature is at 30 DEG C or more in step (3) Bag is without that will seal;Suitable quantity of water is sprayed when sphagna is slightly dry, sphagna of being subject to is held with hand not to be discharged.
Preferably, in step (3), in sphagna vernalization operating process, ponding is not contained in container and polybag, is otherwise easy Lead to seed decay.
Preferably, in step (3), the container includes box, bottle, jar, chest.
Preferably, in step (4), the mass ratio of matrix medium loam and humus is 1:1.
The beneficial effects of the present invention are:
1, pass through dissection, observation, it is determined that fruit and seed mature characteristic provide foundation for the acquisition of ripening fruits.Its Middle mellow fruit is the fruit that exocarp is celadon, and the kind skin of seed is light brown or dark brown leopard grain pattern film quality, endosperm Slightly sclerotin or sclerotin.
2, Baoshan madder needs fresh seeds to sow, and germination is difficult after seed drying water content reduces, and germination percentage is low.
3, sphagna is natural prodcuts, and completely, no germ can be reduced the generation of pest and disease damage;Water-retaining property, drainage, permeability It is good, promote germination.
4, root is directly supported in sphagna, when radicle is long to have root hair, radicle axis green to 3 ± 0.3cm, radicle, transplanting is to educating In seedling container, wherein the root with root hair is planted in ventilative seedling medium, the thick stock cream of radicle axis and package cotyledon exposes nursery Outside matrix, without the cotyledon talent arch soil time, cotyledon and true leaf time of origin are shortened, shortens growing-seedling period, reduce seed in soil In residence time, avoid because soil moisture it is improper caused by rot, substantially increase emergence rate, nursery stock germination is neat, easily Management.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with It obtains other drawings based on these drawings.
Fig. 1 is the comparison photo of Baoshan madder fruit and seed mature characteristic in the present invention;
Fig. 2 is the photo of Baoshan madder seed sphagna vernalization of the present invention;
Fig. 3 is the photo after Baoshan madder of the present invention transplanting.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention, Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention one Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of creative work.
Embodiment 1:
A kind of method of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling, comprising the following steps:
(1) acquisition of fruit and seed: acquisition exocarp is the mature Baoshan madder fruit of celadon;It will be outer with sharp knife Pericarp removing;Taking out kind of skin is light brown or dark brown leopard grain pattern film quality, endosperm slightly sclerotin or sclerotin Baoshan madder seed.
Wherein the comparison of Baoshan madder mellow fruit and seed and jejune fruit and seed is as shown in Figure 1.
(2) seed disinfection: the fresh seeds of taking-up are cleaned up with clear water, then the Gao Meng for being 0.1% with mass concentration Sour potassium rinses spare after impregnating 30 minutes.
(3) sphagna vernalization: solid carbon dioxide tongue fur being placed in water and is impregnated with, spare after can not holding moisture with hand after pulling out;
Prepare a sealable container or sealable polybag.
When using container, the layer overlay sphagna in container, then the fresh Baoshan that wash clean will be sterilized through step (2) are alizarin Seed is layered on sphagna, then then at one layer of the seed upper berth completed sphagna, then sealing container.Wherein container includes box, bottle Son, jar, chest etc..
When using polybag, the layer overlay sphagna in polybag, then the fresh guarantor of wash clean will be sterilized through step (2) Mountain madder seed is layered on sphagna, then then at one layer of the seed upper berth completed sphagna, then sealed plastic bag.
It, can be to container or polybag without to seal to avoid seed bored bad when environment temperature is at 30 DEG C or more;Water Suitable quantity of water is sprayed when tongue fur is slightly dry, sphagna of being subject to is held with hand not to be discharged.In sphagna vernalization operating process, in container and polybag not Containing ponding, it is otherwise easy to cause seed decay.
(4) preparation of transplanting medium: in being put into seedling medium in container for plant growth, seedling medium is the mixed of loam and humus Matrix (mass ratio of loam and humus is 1:1) is closed, transplanting the previous day sprinkles profoundly water seedling medium.
(5) it transplants: directly supporting root in sphagna and moved when radicle is long to have root hair, radicle axis green to 3 ± 0.3cm, radicle It plants to container for plant growth, wherein the root with root hair is planted in ventilative seedling medium, the thick stock cream dew of radicle axis and package cotyledon Out outside seedling medium, 800 times of liquid of 50% carbendazim, 800 times of liquid or 75% Bravo are then poured.
(6) it manages: keeping ground moistening, 800 times of liquid of 50% carbendazim or 75% Bravo 800 were sprayed every 7 days Times liquid, emerges after 20 days.
Seedling growth test:
Test example 1:
Carrying out the influence of Baoshan madder fresh seeds, the seed that dries in the shade to germination percentage in April, 2019, (i.e. seed moisture content is to germination The influence of rate).Wherein fresh seeds water content 40%, dry in the shade seed moisture content 3.9%, each 100, is put in 25 DEG C of temperature setting Incubator in.
Acquire the fruit of exocarp celadon;Exocarp is removed with sharp knife, it is light brown or deep brown for taking out kind of skin The seed of the leopard grain pattern film quality of color, endosperm slightly sclerotin or sclerotin.Wash seeds are clean, 30 are impregnated with 0.1% potassium permanganate It is rinsed after minute spare.A part is directly used sphagna vernalization (fresh seeds water content 40%) using the method in the present invention, and one Part uses the method in the present invention with sphagna vernalization after drying in the shade (dry in the shade seed moisture content 3.9%).Different Baoshan madder seeds contain Water germination is shown in Table 1.
1 Baoshan madder seed moisture content germination of table
As shown in Table 1, on the basis of using identical sphagna germination accelerating method, using fresh Baoshan madder fresh seeds vernalization When, germination percentage will be apparently higher than the seed after drying in the shade, up to 95%, and the corresponding germination percentage of the seed that dries in the shade only has 28%, together Its germinating time seed that relatively dries in the shade significantly reduces when the fresh Baoshan madder fresh seeds vernalization of Shi Caiyong.Therefore Baoshan madder needs fresh Seed sowing, germination is difficult after seed drying water content reduces, and germination percentage is low.
Test example 2:
Carry out different Baoshan madder wet sand vernalization program requests, influence of the sphagna vernalization transplanting method to nursery in April, 2019.
Acquire the fruit of exocarp celadon;Exocarp is removed with sharp knife, it is light brown or deep brown for taking out kind of skin The seed of the leopard grain pattern film quality of color, endosperm slightly sclerotin or sclerotin;Wash seeds are clean, 30 are impregnated with 0.1% potassium permanganate It is rinsed after minute spare.It each 100, seed, is carried out in greenhouse.
Wet sand vernalization point broadcast mode: the seed of washing and sterilizing is stored in flowerpot in the mixing of the ratio of river sand and seed 3:1. I.e. agglomerating, the i.e. scattered middle humidity of light pressure is pinched in sand requirement with hand.
Sphagna vernalization transplanting method: using the method for the rapidly and efficiently Germination And Seedling of Baoshan madder in the embodiment of the present invention 1, sphagna Vernalization transplanting method are as follows: solid carbon dioxide tongue fur is placed in water and is impregnated with, it is spare after can not holding moisture with hand after pulling out;It is spread in polybag One layer of sphagna, then the fresh seeds for sterilizing wash clean are layered on sphagna, then at one layer of the seed upper berth completed sphagna, sealing modeling Material bag, sphagna vernalization are as shown in Figure 2.In being put into seedling medium in container for plant growth, seedling medium is the mixing of loam and humus Matrix (mass ratio of loam and humus is 1:1), transplanting the previous day sprinkles profoundly water seedling medium.Root is directly supported in sphagna, When radicle is long to have root hair, radicle axis green to 3 ± 0.3cm, radicle, in transplanting to container for plant growth, wherein the root with root hair plant into In ventilative seedling medium, the thick stock cream of radicle axis and package cotyledon exposes outside seedling medium, then pours 800 times of 50% carbendazim 800 times of liquid of liquid or 75% Bravo.It is alizarin as shown in Figure 3 using the Baoshan after sphagna vernalization transplanting method transplanting.
The test result of different vernalization and transplanting method is shown in Table 2.
The test result of the different vernalization of table 2 and transplanting method
As shown in Table 2, sphagna vernalization transplanting method of the invention is compared with conventional wet sand vernalization point broadcast mode, germination percentage 25% is improved, and germinating time is appreciably shorter, while cotyledon goes out the time and obviously shifts to an earlier date, it is seen that sphagna vernalization transplanting of the invention Mode is substantially better than wet sand vernalization point broadcast mode.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although with reference to the foregoing embodiments Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation Technical solution documented by example is modified or equivalent replacement of some of the technical features;And these modification or Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.

Claims (6)

1. a kind of method of rare or endangered species Baoshan madder rapidly and efficiently Germination And Seedling, which comprises the following steps:
(1) acquisition of fruit and seed: acquisition exocarp is the Baoshan madder fruit of celadon;Exocarp is shelled with sharp knife From;Taking out kind of skin is light brown or dark brown leopard grain pattern film quality, endosperm slightly sclerotin or sclerotin Baoshan madder seed;
(2) seed disinfection;
(3) sphagna vernalization: solid carbon dioxide tongue fur being placed in water and is impregnated with, spare after can not holding moisture with hand after pulling out;
Prepare a sealable container or sealable polybag, the layer overlay sphagna in container or polybag, then will disappear The fresh Baoshan madder seed of malicious wash clean is layered on sphagna, then then at one layer of the seed upper berth completed sphagna, then sealing container Or polybag;
(4) preparation of transplanting medium: in being put into seedling medium in container for plant growth, seedling medium is the mixed base of loam and humus Matter, transplanting the previous day sprinkle profoundly water seedling medium;
(5) it transplants: directly supporting root in sphagna, when radicle is long to have root hair, radicle axis green to 3 ± 0.3cm, radicle, transplanting is extremely In container for plant growth, wherein the root with root hair is planted in ventilative seedling medium, the thick stock cream exposing of radicle axis and package cotyledon is educated Outside seedling matrix, 800 times of liquid of 50% carbendazim, 800 times of liquid or 75% Bravo are then poured;
(6) it manages: keeping ground moistening, 800 times of liquid of 800 times of liquid of 50% carbendazim or 75% Bravo were sprayed every 7 days, It emerges after 20 days.
2. the method for the rare or endangered species Baoshan according to claim 1 madder rapidly and efficiently Germination And Seedling, which is characterized in that In step (2), seed disinfection specifically: the fresh seeds of taking-up are cleaned up with clear water, then with mass concentration are 0.1% Potassium permanganate rinses spare after impregnating 30 minutes.
3. the method for the rare or endangered species Baoshan according to claim 1 madder rapidly and efficiently Germination And Seedling, which is characterized in that It,, can be to container or polybag without to seal to avoid seed bored bad when environment temperature is at 30 DEG C or more in step (3); Suitable quantity of water is sprayed when sphagna is slightly dry, sphagna of being subject to is held with hand not to be discharged.
4. the method for the rare or endangered species Baoshan according to claim 1 madder rapidly and efficiently Germination And Seedling, which is characterized in that In step (3), in sphagna vernalization operating process, ponding is not contained in container and polybag, is otherwise easy to cause seed decay.
5. the method for the rare or endangered species Baoshan according to claim 1 madder rapidly and efficiently Germination And Seedling, which is characterized in that In step (3), the container includes box, bottle, jar, chest.
6. the method for the rare or endangered species Baoshan according to claim 1 madder rapidly and efficiently Germination And Seedling, which is characterized in that In step (4), the mass ratio of matrix medium loam and humus is 1:1.
CN201910708519.8A 2019-08-01 2019-08-01 Method for rapid and efficient germination and seedling formation of rare or endangered plant Rubia Baoshanensis Expired - Fee Related CN110249926B (en)

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Inventor after: Geng Yunfen

Inventor after: Li Jing

Inventor after: Yu Lizhi

Inventor after: Yang Qiang

Inventor after: Zhang Jinfeng

Inventor after: Yang Changji

Inventor after: Geng Shaodong

Inventor before: Geng Yunfen

Inventor before: Li Jing

Inventor before: Yu Lizhi

Inventor before: Yang Qiang

Inventor before: Zhang Jinfeng

Inventor before: Yang Changji

Inventor before: Geng Shaodong

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Granted publication date: 20210917