CN204762668U - Group banks up explant disinfection and dewatering device with earth - Google Patents
Group banks up explant disinfection and dewatering device with earth Download PDFInfo
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- CN204762668U CN204762668U CN201520500814.1U CN201520500814U CN204762668U CN 204762668 U CN204762668 U CN 204762668U CN 201520500814 U CN201520500814 U CN 201520500814U CN 204762668 U CN204762668 U CN 204762668U
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- screen cloth
- stack shell
- explant
- holding rod
- cloth stack
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Abstract
The utility model discloses a group banks up explant disinfection and dewatering device with earth, including the holding rod that is used for splendid attire explant and permeable to water screen cloth stack shell, is connected with the screen cloth stack shell and central foraminiferous screen cloth approximately, screen cloth lid threaded connection in the upper portion of screen cloth stack shell, screen cloth lid, threaded connection in the bottom of screen cloth stack shell are passed to holding rod one end. The utility model discloses simple structure, convenient operation can effectively prevent the loss of explant at the disinfection in -process to accessible time the dehydration is in order to prevent brownization.
Description
Technical field
The utility model relates to agricultural class field of plant tissue culture technique, specifically a kind of group training explant sterilization and dewater unit.
Background technology
Explant is the organ or tissue's fragment as cultured in vitro material in Plant Tissue Breeding, usually outdoor plant live body is taken from, a large amount of microorganism can be adhered in surface, in incubation, outer planting body surface miscellaneous bacteria is killed if do not sterilize, various miscellaneous bacteria Fast-propagation, not only seizes explant nutrient and also can produce exotoxin, affect explant growth.Therefore, before being inoculated on medium, strict sterilization must be carried out.
Explant sterilization is usually first through running water such as running water, soak with 75% alcohol short time afterwards, soak with 0.1% mercuric chloride or 10% clorox long period again after sterile water fully rinses, in immersion process, frequently rock stirring, and again use aseptic water washing 3 times.Said process is loaded down with trivial details, need repeatedly to use tweezers and prevent explant from flowing away with thimerosal or sterile water and produce loss, but effect is unsatisfactory, has loss to cause scientific research waste of material unavoidably.
Need with tweezers gripping explant (being often plant leaf blade) in aseptic water washing process, explant is often caused to stab, easily cause Brown, be unfavorable for that the later stage cultivates, and after aseptic water washing, usually explant is transferred in a container, inoculate, but due to the action need time, explant can be immersed in for a long time in the sterile water fully do not inclined before inoculation in batches, cause external body water logging undue, cause late stage of culture brownization.
Although have some about the announcement of explant surface sterilization device, if Chinese patent Authorization Notice No. is CN204191321U, CN203814333U, CN203043041U, CN203040347U, CN202232459U, CN102018969B, CN201453700U etc., but most of complex structure, parts too much fragile, manufacturing cost is high or complicated operation, be unfavorable for actual use, and all can not fully remove explant surface moisture in time, prevent brownization in explant later stage incubation.
Utility model content
The purpose of this utility model is to provide that a kind of structure is simple, the group of handled easily training explant sterilization and dewater unit.
For achieving the above object, the utility model provides following technical scheme:
A kind of group training explant sterilization and dewater unit, comprise for the splendid attire explant screen cloth lid with holes with permeable screen cloth stack shell, the holding rod be connected with screen cloth stack shell and center; Described screen cloth lid is threadedly connected to the top of screen cloth stack shell; Described holding rod one end, through screen cloth lid, is threadedly connected to the bottom of screen cloth stack shell.
As further program of the utility model: the bottom of described screen cloth stack shell is cone shape, and bottom apex place is provided with the connecting thread corresponding with holding rod screw thread.
As further program of the utility model: the length of described holding rod is 28-40cm, one end is provided with the connecting thread corresponding with screen cloth stack shell bottom apex place screw thread.
As further program of the utility model: the length of described holding rod is 30cm.
As further program of the utility model: the middle part of described screen cloth lid has through hole.
As further program of the utility model: described device and diameter be greater than screen cloth stack shell diameter 3-6cm, highly doubly add a cover rear height to screen cloth stack shell for 3-6 beaker with the use of.
As further program of the utility model: described device and diameter be greater than screen cloth stack shell diameter 4cm, be highly 4 times of beakers of adding a cover rear height to screen cloth stack shell with the use of.
Compared with prior art, the beneficial effects of the utility model are: the utility model structure is simple, easy to operate, effectively can prevent the loss of explant in disinfecting process, and can dewater in time to prevent brownization.
Accompanying drawing explanation
Fig. 1 is the structural representation of group training explant sterilization and dewater unit;
Fig. 2 is the fractionation structural representation of group training explant sterilization and dewater unit;
In figure: 1-holding rod, 2-screen cloth lid, 3-screen cloth stack shell, 4-bottom apex.
Embodiment
Below in conjunction with the utility model embodiment, be clearly and completely described the technical scheme in the utility model embodiment, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
Refer to Fig. 1 ~ 2, in the utility model embodiment, a kind of group training explant sterilization and dewater unit, comprise for the splendid attire explant screen cloth lid 2 with holes with permeable screen cloth stack shell 3, the holding rod 1 be connected with screen cloth stack shell and center; Screen cloth lid 2 is threadedly connected to the top of screen cloth stack shell 3; One end of holding rod 1, through screen cloth lid 2, is threadedly connected to the bottom of screen cloth stack shell 3; The bottom of screen cloth stack shell 3 is cone shape, and is provided with the connecting thread corresponding with screw thread on holding rod 1 at bottom apex 4 place; The length of holding rod 1 is 28-40cm, preferred 30cm, and one end of holding rod 1 is provided with the connecting thread corresponding with the bottom apex 4 place screw thread of screen cloth stack shell 3; The middle part of screen cloth lid has through hole, and its diameter, slightly larger than the external diameter of holding rod 1, makes holding rod 1 can pass from centre.
The diameter 3-6cm that the utility model need be greater than screen cloth stack shell 3 with diameter, highly doubly add a cover rear height to screen cloth stack shell 3 for 3-6 beaker with the use of.Preferably be greater than with diameter the diameter 4cm of screen cloth stack shell 3, be highly 4 times of beakers of adding a cover rear height to screen cloth stack shell 3 with the use of.
The utility model is when carrying out explant sterilization, and its course of work is as follows:
(1) first getting explant loads in screen cloth stack shell 3, threaded for holding rod 1 one end is screwed in bottom apex 4 place of screen cloth stack shell 3, be connected with screen cloth stack shell 3, then the through hole of screen cloth lid 2 through holding rod 1, be screwed on screen cloth stack shell 3, can prepare after installation to start sterilization.
(2) conveniently explant disinfection process, under holding rod 1 is tethered in water tap, running water is after 30 minutes, and whole device is placed in superclean bench.
(3) device is put into the empty beaker of 1000mL, be kept upright, the alcohol adding 75% in beaker floods to screen cloth stack shell 3, rinses after washing 30 seconds, withdrawing device, alcohol is outwelled, the sterile water of beaker volume 1/3rd is added again in beaker, after embedding device is repeatedly rinsed and is washed, withdrawing device, outwell sterile water to waste liquid cylinder, sterile water is rinsed and is washed repetition 3 times.
(4) device is put into the empty beaker of 1000mL, pouring 0.1% mercuric chloride solution into flooding screen cloth stack shell 3, soaking 8 minutes, washing and sieving net stack shell 3 rinsed by the every 2 minutes needs in midway, ensures that solution fully contacts explant; After immersion completes, withdrawing device, outwells mercuric chloride solution to waste liquid cylinder, then in beaker, adds sterile water to three/mono-volume place, embedding device, repeatedly rinses after washing, withdrawing device, outwells sterile water to waste liquid cylinder, and sterile water is rinsed and washed repetition 3 times.
(5) device is put into empty 1000mL beaker, two palms clamp the top of holding rod 1, left-hand thread direction rubbing holding rod 1, drive screen cloth stack shell 3 quick rotation, utilize centrifugal force to be thrown away by the liquid in cylinder, to dewater.
(6) after dehydration, screen cloth stack shell 3 is put in and is covered with in the culture dish of filter paper, then the filter paper getting one piece of dry sterilization encases screen barrel body 3, rotate and extract holding rod 1 out, open screen cloth lid 2, take out explant for subsequent use.
The utility model structure is simple, easy to operate, effectively can prevent the loss of explant in disinfecting process, and can dewater in time to prevent brownization.
To those skilled in the art, obvious the utility model is not limited to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present utility model or essential characteristic, can realize the utility model in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present utility model is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the utility model.
In addition, be to be understood that, although this specification is described according to embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of specification is only for clarity sake, those skilled in the art should by specification integrally, and the technical scheme in each embodiment also through appropriately combined, can form other embodiments that it will be appreciated by those skilled in the art that.
Claims (7)
1. the sterilization of group training explant and a dewater unit, is characterized in that, comprise for the splendid attire explant screen cloth lid with holes with permeable screen cloth stack shell, the holding rod be connected with screen cloth stack shell and center; Described screen cloth lid is threadedly connected to the top of screen cloth stack shell; Described holding rod one end, through screen cloth lid, is threadedly connected to the bottom of screen cloth stack shell.
2. the sterilization of according to claim 1 group of training explant and dewater unit, it is characterized in that, the bottom of described screen cloth stack shell is cone shape, and bottom apex place is provided with the connecting thread corresponding with holding rod screw thread.
3. the sterilization of according to claim 2 group of training explant and dewater unit, it is characterized in that, the length of described holding rod is 28-40cm, and one end is provided with the connecting thread corresponding with screen cloth stack shell bottom apex place screw thread.
4. the sterilization of according to claim 3 group of training explant and dewater unit, it is characterized in that, the length of described holding rod is 30cm.
5. the sterilization of according to claim 1 group of training explant and dewater unit, it is characterized in that, the middle part of described screen cloth lid has through hole.
6. the sterilization of according to claim 1 group of training explant and dewater unit, is characterized in that, described device and diameter be greater than screen cloth stack shell diameter 3-6cm, highly be the 3-6 times beaker of adding a cover rear height to screen cloth stack shell with the use of.
7. the sterilization of according to claim 6 group of training explant and dewater unit, is characterized in that, described device and diameter be greater than screen cloth stack shell diameter 4cm, be highly 4 times of beakers of adding a cover rear height to screen cloth stack shell with the use of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520500814.1U CN204762668U (en) | 2015-07-10 | 2015-07-10 | Group banks up explant disinfection and dewatering device with earth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520500814.1U CN204762668U (en) | 2015-07-10 | 2015-07-10 | Group banks up explant disinfection and dewatering device with earth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204762668U true CN204762668U (en) | 2015-11-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520500814.1U Withdrawn - After Issue CN204762668U (en) | 2015-07-10 | 2015-07-10 | Group banks up explant disinfection and dewatering device with earth |
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| CN (1) | CN204762668U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904604A (en) * | 2015-07-10 | 2015-09-16 | 四川农业大学 | Disinfection and dewatering device for tissue culture of explants |
| CN107455260A (en) * | 2017-09-13 | 2017-12-12 | 西南大学 | Xylophyta explant stem section sterilizer |
-
2015
- 2015-07-10 CN CN201520500814.1U patent/CN204762668U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904604A (en) * | 2015-07-10 | 2015-09-16 | 四川农业大学 | Disinfection and dewatering device for tissue culture of explants |
| CN104904604B (en) * | 2015-07-10 | 2018-03-06 | 四川农业大学 | A kind of tissue culture explant sterilization and dehydration device |
| CN107455260A (en) * | 2017-09-13 | 2017-12-12 | 西南大学 | Xylophyta explant stem section sterilizer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20151118 Effective date of abandoning: 20180306 |
|
| AV01 | Patent right actively abandoned |