CN107455260A - Xylophyta explant stem section sterilizer - Google Patents
Xylophyta explant stem section sterilizer Download PDFInfo
- Publication number
- CN107455260A CN107455260A CN201710819868.8A CN201710819868A CN107455260A CN 107455260 A CN107455260 A CN 107455260A CN 201710819868 A CN201710819868 A CN 201710819868A CN 107455260 A CN107455260 A CN 107455260A
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- China
- Prior art keywords
- bottle
- xylophyta
- explant
- connector
- stem section
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of xylophyta explant stem section sterilizer, including bottle, the filtering rack for being used for containing explant is provided with the bottle, leaves gap between the bottom surface and the bottle bottom surface of the filtering rack, the side wall of the bottle is provided with osculum；The top of the bottle is provided with connector, the upper end of the connector is provided with the connector for being used for being sleeved on tap, array is provided with least two fasteners for being used to be fixed on the connector on tap in the side wall of the connector, and the lower end of the connector is connected with the bottle.The xylophyta explant stem section sterilizer of the present invention, can effectively remove most of germ reduces pollution rate, and can uniformly rinse explant by running water, is produced with reducing quinones substance caused by explant incision and then preventing and treating browning.
Description
Technical field
The present invention relates to a kind of sterilizer, is specifically a kind of xylophyta explant stem section sterilizer.
Background technology
In plant tissue culture course, explant is generally collected in outdoor plant as tissue cultures major experimental material
Thing live body.Because long-time is exposed in natural environment, plant surface carries substantial amounts of bacterium and fungi（Influence tissue cultures
Two big mushrooms of success or failure）If being cleared up without surface contamination, various microorganisms will quickly breed after inoculation, have a strong impact on group
Knit the success rate of culture.
Xylophyta is a kind of well differentiated perennial plant, and the miscellaneous bacteria adhered to thereon is numerous, if only chemically
Processing, is extremely difficult to preferable Disinfection Effect.Therefore, before chemical disinfection, explant irrigation and disinfection can be effectively reduced
The remaining of bacterium.If by the use of young leaves, bud and spray as explant, due to its in atmosphere open-assembly time it is short, the dirt of material surface
Microbiological contamination also has little time to invade inside explant, now explant surface washing sterilization is easy to thoroughly remove miscellaneous bacteria, reached
To expected Disinfection Effect.Furthermore in the xylophyta of normal growth, phenolic compound, polyphenol oxidase in plant with
And the separation of the material such as tannin is present in maintenance cell physiological ecology in each cell, after explant is cut, the cell of incision
Stimulated by wound, cell micro-environment is interfered, substantial amounts of aldehydes matter be discharged into it is extracellular, then through air oxidation into quinones
Material, if in time the aldehydes matter for being discharged into the external world is washed by running water, can be effectively reduced browning.
Current explant surface sterilization device, as Publication No. CN204191321U, CN204762668U,
CN203814333U, CN203043041U and CN202232459U etc., although disclosure satisfy that requirement to a certain extent,
Exist complicated, part it is excessive it is fragile, manufacturing cost is high, complex operation or current are not running water, be not easy to actually make
With the defects of, and can not fully exclude to clean explant in time and contain miscellaneous bacteria and quinones substance rinse water, not reach expection
Sterilization and cleaning performance.
The content of the invention
In view of this, can be effectively clear it is an object of the invention to provide a kind of xylophyta explant stem section sterilizer
Except most of germ reduces pollution rate, and explant can uniformly be rinsed by running water, to reduce caused by explant incision
Quinones substance and then preventing and treating browning generation.
To reach above-mentioned purpose, the present invention provides following technical scheme：
A kind of xylophyta explant stem section sterilizer, including bottle, the bottle is interior to be provided with the filtering for being used for containing explant
Rack, gap is left between the bottom surface and the bottle bottom surface of the filtering rack, the side wall of the bottle is provided with osculum；
The top of the bottle is provided with connector, and the upper end of the connector is provided with the connector for being used for being sleeved on tap,
Array is provided with least two fasteners for being used to be fixed on the connector on tap in the side wall of the connector, described
The lower end of connector is connected with the bottle.
Further, mouth hanging is provided with the bottle upper end open, the filtering rack is provided with what is coordinated with the mouth hanging
Rack is linked up with.
Further, the mouth hanging annular is uniformly arranged as at least three, and the rack hook corresponds with the mouth hanging
Set.
Further, the bottom of the connector is provided with suspension member, and the suspension member is provided with coupling hook, the top of the bottle
Provided with the hanging hole coordinated with the coupling hook.
Further, the bottom surface of the bottle is to the raised convex bottom of bottle in bottleneck direction.
Further, the osculum is positioned at the lower section of the filtering rack bottom surface.
Further, axial direction of the osculum along the bottle is set to multigroup, and osculum described in each group includes
The multiple osculums uniform along the circumferential direction annular of the bottle.
Further, the height of the bottle is 15cm, internal diameter 3cm, and the spacing between osculum described in two adjacent groups is
1/8cm, the spacing between same group of the two neighboring osculum is 1/8cm.
Further, the height of the filtering rack is 9cm, internal diameter 2.8mm, and the upper array of the filtering rack is provided with
Internal diameter is 1mm sieve aperture.
Further, the fastener uses trip bolt, and described radially inner one end of trip bolt is provided with rubber suction cups.
The beneficial effects of the present invention are：
The xylophyta explant stem section sterilizer of the present invention, by setting filtering rack in bottle, and explant is contained
In filtering rack, tap is connected using connector, opening tap can uniformly rinse to explant progress running water, work as water
When stream reaches certain speed, vortex can be formed in bottle, vortex can form ascending current, under the flushing of upper and lower current, more can
Operatively rinse explant；Moreover, explant can also be reduced using the xylophyta explant stem section sterilizer of the present invention
Body is lost in when running water rinses, mostly important at the same time it can also study influence of the flow velocity of water to explant incision microenvironment
Be that can effectively remove most of germ, and take away quinones substance caused by incision, and then prevent and treat Brown.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below and carried out
Explanation：
Fig. 1 is the structural representation of xylophyta explant stem section sterilizer embodiment of the present invention；
Fig. 2 is the structural representation of bottle；
Fig. 3 is the structural representation of connector；
Fig. 4 is the structural representation of filtering rack；
Fig. 5 is that the use state being arranged on the xylophyta explant stem section sterilizer of the present embodiment when on tap refers to
Figure；
Fig. 6 is the stem section browning prevention effect analysis chart that the sterilizer is used under different water flows；
Fig. 7 is the stem section prevention and cure of pollution effect analysis figure that the sterilizer is used under different water flows；
Fig. 8 is the stem section browning prevention effect analysis chart that the sterilizer is used under different water flows；
Fig. 9 is the stem section prevention and cure of pollution effect analysis figure that the sterilizer is used under different water flows.
Embodiment
The invention will be further described with specific embodiment below in conjunction with the accompanying drawings, so that those skilled in the art can be with
It is better understood from the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
As shown in figure 1, the structural representation for xylophyta explant stem section sterilizer embodiment of the present invention.The present embodiment
Xylophyta explant stem section sterilizer, including bottle 1, be provided with the filtering rack 2 for being used for containing explant, mistake in bottle 1
Gap is left between the bottom surface and the bottom surface of bottle 1 of screen holder 2, the side wall of bottle 1 is provided with osculum 3.The top of bottle 1 is provided with
Connector 4, the upper end of connector 4 are provided with the connector 5 for being used for being sleeved on tap, and array is provided with the side wall of connector 5
At least two fasteners 6 for being fixed on connector on tap, the lower end of connector 4 is connected with bottle 1.The present embodiment
Connector 5 side wall on array be provided with four and be used to for connector to be fixed on fasteners 6 on tap, the fastening of the present embodiment
Part uses trip bolt, and the radially inner one end of trip bolt is provided with rubber suction cups 7.
Further, mouth hanging is provided with the upper end open of bottle 1, filtering rack 2 is provided with the rack hook 8 coordinated with mouth hanging,
Filtering rack 2 can be hung on bottle 1, be easy to dismount.Mouth hanging annular is uniformly arranged as at least three, rack hook 8 and mouth hanging
Correspond and set.The annular of mouth hanging and rack hook 8 of the present embodiment is uniformly set to four.
Further, the bottom of connector 4 is provided with suspension member 8, and suspension member 8 is provided with coupling hook 10,1 top of bottle be provided with
The hanging hole that coupling hook 10 coordinates.Bottle 1 can be mounted on connector 4, be easy to dismount.The bottom of the bottle 1 of the present embodiment
Face is to the raised convex bottom of bottle 13 in bottleneck direction, is easy to water stream and forms vortex.
Further, osculum 3 is positioned at the lower section of filtering rack 2 bottom surface.Axle of the osculum 3 of the present embodiment along bottle 1
It is set to multigroup to direction, each group of osculum 3 is included along the uniform multiple osculums of the circumferential direction annular of bottle 1.This reality
The height for applying the bottle 1 of example is 15cm, internal diameter 3cm, and the spacing between two adjacent groups osculum 3 is 1/8cm, same group of phase
Spacing between adjacent two osculums 3 is 1/8cm.
Further, the height of the filtering rack 2 of the present embodiment is 9cm, internal diameter 2.8mm, and filters the upper array of rack 2
Provided with the sieve aperture 12 that internal diameter is 1mm.
At least three connecting rods that the connector of the present embodiment is uniformly arranged using annular surround, the top of all connecting rods
The connector 5 coordinated with tap 11 is surrounded, lower end bending expansion coordinates with bottle 1, and connects firmware 8.
The xylophyta explant stem section sterilizer of the present embodiment, by setting filtering rack in bottle, and by explant
Body is contained in filtering rack, and tap is connected using connector, and opening tap can uniformly rush to explant progress running water
Wash, when current reach certain speed, vortex can be formed in bottle, vortex can form ascending current, in the flushing of upper and lower current
Under, it more can operatively rinse explant；Moreover, can also be subtracted using the xylophyta explant stem section sterilizer of the present invention
Few explant is lost in when running water rinses, at the same time it can also study influence of the flow velocity of water to explant incision microenvironment, most
For it is important that most of germ can effectively be removed, and quinones substance caused by incision is taken away, and then it is brown to prevent and treat explant
Change.
Specifically, the data analysis structure of explant is cleaned using the xylophyta explant stem section sterilizer of the present embodiment
As shown in Fig. 6-Fig. 9, can effectively remove most of germ reduces pollution rate, and can uniformly rinse explant by running water,
Produced with reducing quinones substance caused by explant incision and then preventing and treating browning.
Embodiment described above is only to absolutely prove preferred embodiment that is of the invention and being lifted, protection model of the invention
Enclose not limited to this.The equivalent substitute or conversion that those skilled in the art are made on the basis of the present invention, in the present invention
Protection domain within.Protection scope of the present invention is defined by claims.
Claims (10)
- A kind of 1. xylophyta explant stem section sterilizer, it is characterised in that：It is provided with including bottle, in the bottle and is used to contain The filtering rack of explant, gap, the side wall of the bottle are left between the bottom surface and the bottle bottom surface of the filtering rack It is provided with osculum；The top of the bottle is provided with connector, and the upper end of the connector is provided with the connector for being used for being sleeved on tap, Array is provided with least two fasteners for being used to be fixed on the connector on tap in the side wall of the connector, described The lower end of connector is connected with the bottle.
- 2. xylophyta explant stem section sterilizer according to claim 1, it is characterised in that：The bottle upper end open Place is provided with mouth hanging, and the filtering rack is provided with the rack coordinated with the mouth hanging and linked up with.
- 3. xylophyta explant stem section sterilizer according to claim 2, it is characterised in that：The mouth hanging annular is uniform At least three are arranged to, the rack hook corresponds with the mouth hanging to be set.
- 4. xylophyta explant stem section sterilizer according to claim 1, it is characterised in that：The bottom of the connector Provided with suspension member, the suspension member is provided with coupling hook, and the top of the bottle is provided with the hanging hole coordinated with the coupling hook.
- 5. xylophyta explant stem section sterilizer according to claim 1, it is characterised in that：The bottom surface of the bottle is The convex bottom of bottle raised to bottleneck direction.
- 6. xylophyta explant stem section sterilizer according to claim 1, it is characterised in that：The osculum is located at institute State the lower section of filtering rack bottom surface.
- 7. xylophyta explant stem section sterilizer according to claim 6, it is characterised in that：The osculum is along institute The axial direction for stating bottle is set to multigroup, and osculum described in each group includes uniform along the circumferential direction annular of the bottle Multiple osculums.
- 8. xylophyta explant stem section sterilizer according to claim 7, it is characterised in that：The height of the bottle is 15cm, internal diameter 3cm, the spacing between osculum described in two adjacent groups are 1/8cm, same group of the two neighboring osculum Between spacing be 1/8cm.
- 9. xylophyta explant stem section sterilizer according to claim 8, it is characterised in that：The height of the filtering rack Spend for 9cm, internal diameter 2.8mm, and the upper array of the filtering rack is provided with the sieve aperture that internal diameter is 1mm.
- 10. xylophyta explant stem section sterilizer according to claim 1, it is characterised in that：The fastener uses Trip bolt, described radially inner one end of trip bolt are provided with rubber suction cups.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710819868.8A CN107455260A (en) | 2017-09-13 | 2017-09-13 | Xylophyta explant stem section sterilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710819868.8A CN107455260A (en) | 2017-09-13 | 2017-09-13 | Xylophyta explant stem section sterilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107455260A true CN107455260A (en) | 2017-12-12 |
Family
ID=60552197
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710819868.8A Pending CN107455260A (en) | 2017-09-13 | 2017-09-13 | Xylophyta explant stem section sterilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107455260A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108668895A (en) * | 2018-04-24 | 2018-10-19 | 贵州大学 | A kind of tissue cultures explant flusher and its application method |
| CN112616678A (en) * | 2021-01-20 | 2021-04-09 | 南通大学 | Plant stem segment explant inoculator |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202232459U (en) * | 2011-10-14 | 2012-05-30 | 平顶山学院 | Explant sterilizing device applicable to tissue culture |
| CN203043041U (en) * | 2013-02-07 | 2013-07-10 | 浙江诚邦园林股份有限公司 | Explant sterilizing device |
| CN203538050U (en) * | 2013-09-16 | 2014-04-16 | 上海市农业科学院 | Cleaning sterilizer for tissue culture explant of aquatic plant |
| CN204191321U (en) * | 2014-09-25 | 2015-03-11 | 武汉沃田生态科技有限公司 | A kind of fountain Explant sterilizing device |
| CN204762668U (en) * | 2015-07-10 | 2015-11-18 | 四川农业大学 | Group banks up explant disinfection and dewatering device with earth |
| CN204811398U (en) * | 2015-08-03 | 2015-12-02 | 四川省植物工程研究院 | Wash cup |
| CN204888280U (en) * | 2015-08-13 | 2015-12-23 | 中国热带农业科学院南亚热带作物研究所 | Plant tissue culture explant degassing unit |
| CN205830696U (en) * | 2016-07-27 | 2016-12-28 | 四川农业大学 | A kind of group trains outer implant sterilization bottle |
-
2017
- 2017-09-13 CN CN201710819868.8A patent/CN107455260A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202232459U (en) * | 2011-10-14 | 2012-05-30 | 平顶山学院 | Explant sterilizing device applicable to tissue culture |
| CN203043041U (en) * | 2013-02-07 | 2013-07-10 | 浙江诚邦园林股份有限公司 | Explant sterilizing device |
| CN203538050U (en) * | 2013-09-16 | 2014-04-16 | 上海市农业科学院 | Cleaning sterilizer for tissue culture explant of aquatic plant |
| CN204191321U (en) * | 2014-09-25 | 2015-03-11 | 武汉沃田生态科技有限公司 | A kind of fountain Explant sterilizing device |
| CN204762668U (en) * | 2015-07-10 | 2015-11-18 | 四川农业大学 | Group banks up explant disinfection and dewatering device with earth |
| CN204811398U (en) * | 2015-08-03 | 2015-12-02 | 四川省植物工程研究院 | Wash cup |
| CN204888280U (en) * | 2015-08-13 | 2015-12-23 | 中国热带农业科学院南亚热带作物研究所 | Plant tissue culture explant degassing unit |
| CN205830696U (en) * | 2016-07-27 | 2016-12-28 | 四川农业大学 | A kind of group trains outer implant sterilization bottle |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108668895A (en) * | 2018-04-24 | 2018-10-19 | 贵州大学 | A kind of tissue cultures explant flusher and its application method |
| CN112616678A (en) * | 2021-01-20 | 2021-04-09 | 南通大学 | Plant stem segment explant inoculator |
| CN112616678B (en) * | 2021-01-20 | 2021-10-08 | 南通大学 | Plant stem segment explant inoculator |
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| PB01 | Publication | ||
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Application publication date: 20171212 |