CN203378377U - Novel potato tissue culture container - Google Patents

Novel potato tissue culture container Download PDF

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Publication number
CN203378377U
CN203378377U CN201320034829.4U CN201320034829U CN203378377U CN 203378377 U CN203378377 U CN 203378377U CN 201320034829 U CN201320034829 U CN 201320034829U CN 203378377 U CN203378377 U CN 203378377U
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CN
China
Prior art keywords
lid
potato
tissue culture
culture vessel
culture container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201320034829.4U
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Chinese (zh)
Inventor
陈亮
柳俊
朱洲
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HUBEI BIO-GREAT AGRICULTURAL TECHNOLOGY Co Ltd
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HUBEI BIO-GREAT AGRICULTURAL TECHNOLOGY Co Ltd
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Priority to CN201320034829.4U priority Critical patent/CN203378377U/en
Application granted granted Critical
Publication of CN203378377U publication Critical patent/CN203378377U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses a novel potato tissue culture container and in particular relates to a novel potato tissue culture container which is made of plastic and is suitable for potato test-tube plantlet and microtuber industrial production. The culture container is made into a square which has a large upper opening and a small lower bottom as well as 1/6 circular corners according to a production requirement. According to the culture container, the operation procedure of potato test-tube plantlet and microtuber production is simplified, repeated use, machine washing, storage, culture and transportation are facilitated, the cost is low, and the pollution rate is low. The culture container has a fixed shape, is suitable for solid and liquid culture and has positive significance for reduction of the industrial production cost of potato test-tube plantlets and microtubers.

Description

A kind of Novel potato tissue culture vessel
Technical field
The utility model potato tissue culture vessel relates generally to a kind of plant tissue culture vessel's body and cover body structure of plastics, relevant with test tube potato Fast-propagation with potato test-tube plantlet.
Background technology
About the existing a lot of reports of plant tissue culture vessel, but produce certain defect is arranged for the batch production of potato test-tube plantlet and test tube potato: early stage plant tissue culture bottle is generally made Bottle structure with glass material, for example: " a kind of blake bottle structure " (patent No.: ZL 95244625.1) glass bottle is frangible, heavy, is not suitable for tissue and cultivates operation and logistics transportation.The plastic material bottle, firmly, light, replace glass with plastic material gradually, blake bottle afterwards is to make with the plastics macromolecular material mostly, for example: " plant tissue culture bottle " (patent No.: ZL 99252172.6), but bottle and bottle cap are for being threaded, be unfavorable for batch production large-scale production, add in addition ventail; " plant tissue cultivating pot " (ZL 200320111149.4), shortcoming is easy pollution, structure is unfavorable for sterile working, cleans difficulty; " a kind of method of High-efficient Production test tube potato and culture vessel thereof " (patent No.: ZL 02131125.0), at the bottom of the right angle box, be not easy to clean, and is unfavorable for factory's large-scale machines cleaning, and the lid structure, still easily pollute." improved structure of combined cap of plant tissue culture bottle " (patent No.: ZL 200520002788.6) shortcoming can not be reused, and cost is high, and cost is high; (number of patent application: CN 201120275514.X), the complicated cost of Bottle cap structure is high, is unfavorable for cleaning, and blow vent, at top, can only be spread in a single layer, and can not take full advantage of culture space, and easily pollutes " a kind of plant tissue culture bottle ".
Summary of the invention
The purpose of this utility model is to develop a kind of applicable potato test-tube plantlet and needed tissue culture vessel is produced in the batch production of test tube potato, and this culture vessel can reach aseptic culture, can be beneficial to again the ventilation of culture, can greatly improve the space that utilizes of culturing room.
The utility model is achieved through the following technical solutions: a kind of Novel potato tissue culture vessel, described potato tissue comprises potato test-tube plantlet or test tube potato, and described container comprises a plastic cylinder, lid, and described plastic cylinder is transparent, up big and down small, lid is transparent; This plastic cylinder is square, and the inwall turning is 1/6 circular arc; On plastic cylinder, the lower edge of edge, lid all has the gomphosis groove cooperatively interacted, thereby seals by gomphosis groove the zigzag path that docking forms gas circuit between the lower edge of edge, lid on plastic cylinder.
What described lid was used is non-screw type, and Dual-layer groove fit-type is tightly connected.
Be provided with the reinforcement of fixing and ventilative post effect in the inside and outside groove of described lid.
This culture vessel is comprised of cylindrical shell and lid, and macromolecular material is made, and box body is transparent, and light transmission is good, but autoclaving can reuse repeatedly.
The cylindrical shell shape is combined as Fig. 2 by square and circular arc type, and the designed arc-shaped appearance at turning does not have dead angle when culture vessel is cleaned, and is conducive to machine and cleans, and saves artificial.Square is conducive to carrying, shifts.
The design of this culture vessel, improved the structure of original similar products, simple in structure, both increased gas permeability, strengthened again resistant to pollution ability in incubation, also saved artificial.This culture vessel also is applicable to the tissue of other plant and cultivates.This culture vessel cylindrical shell, lid are simple in structure, and cost is low, both be beneficial to batch production produce in the culture vessel machine clean, greatly shorten again and know clearly the medium preparation and turn operating time of seedling, saved artificial.
The accompanying drawing explanation
Fig. 1 is the decomposing schematic representation of the utility model new embodiment potato culture vessel;
Fig. 2 is the vertical view of lid in Fig. 1;
Fig. 3 is lid and cylindrical shell connected mode schematic diagram in Fig. 1;
In figure, embeded slot in the 1-lid; Chimera under 2-; The lower outside chimera of 3-: 4-water jacket reinforcement; The 5-plastic cylinder; The 6-lid; The upper outside eaves piece of 7-; Eaves piece in 8-is upper; 9-inside groove reinforcement; The upper chimera of 10-; The outer embeded slot of 11-lid.
Embodiment
Further illustrate the utility model embodiment below in conjunction with accompanying drawing.
Referring to Fig. 1-Fig. 3, a kind of Novel potato tissue culture vessel of the utility model specific embodiment, described potato tissue comprises potato test-tube plantlet or test tube potato, described container comprises a plastic cylinder 5, lid 6, described plastic cylinder is transparent, up big and down small, and lid is transparent; This plastic cylinder is square, and the inwall turning is 1/6 circular arc; On plastic cylinder 5, the lower edge of edge, lid 6 all has the gomphosis groove cooperatively interacted, thereby seals by gomphosis groove the zigzag path that docking forms gas circuit between the lower edge of edge, lid 6 on plastic cylinder 5.
Further, what described lid was used is non-screw type, and Dual-layer groove fit-type is tightly connected.
Further, be provided with the reinforcement of fixing and ventilative post effect in the inside and outside groove of described lid.
The cylindrical shell of the potato test-tube plantlet culture vessel of the utility model embodiment adopts the macromolecular material of screening, is shaped as cylindrical shell square and the combination of 1/6 arc, both can close-packed arrays when placing, can also guarantee bright and clear, save space, can severally take together during transfer, the convenient transfer.
The utility model embodiment cylindrical shell is connected by double-deck caulking groove design with lid, and the lower interior chimera 2 of cylindrical shell is chimeric with the interior embeded slot 1 of lid, and the lower outside chimera 3 of cylindrical shell is chimeric with the outer embeded slot 11 of lid.In embeded slot and outer embeded slot, reinforcing rib design is all arranged in lid, play ventilative post simultaneously.The particular design that this lid is connected with cylindrical shell is as Fig. 3, and the gas circuit of lid is " bow " font, has both guaranteed germ-free condition, has guaranteed again the gas permeability requirements of plant growth.Simultaneously, easily open and close, facilitating the preparation of medium and turn the sterile working in seedling.
When the utility model embodiment cultivates potato test-tube plantlet and test tube potato, the concrete operations of culture vessel are as follows:
At first medium is prepared, use knockout, packing 30 ~ 40ml medium in each culture vessel, cover lid and compress, and two embeded slot lids have guaranteed in sterilization process, and lid is not easy to be flicked by steam.After sterilizing, in desinfection chamber, set level, cooling, parked 2-3 days turns seedling on aseptic operating platform, then in transferring between illumination cultivation, on culturing rack, cultivates until test-tube plantlet meets the requirements or ties the potato results.Then foreign material in box are poured out, be placed on the rack for cleaning of cleaning robot, automatically clean.Finally, the back-off that staggers, dry, and enters next production cycle.
Embodiment 1:
1. the structure of culture vessel is with reference to Fig. 1, and culture vessel is square circular, cylindrical shell polycarbonate plastic tank, lid polypropylene material, the long 4cm of straight flange on cylindrical shell four limits, high 10cm, turning 1/6 circular arc;
2. use knockout, in each culture vessel, packing 30 ~ 40ml medium after sterilizing, is set level in desinfection chamber, cooling, parked 2-3 days.Then, on aseptic operating platform, open the lid, by sterile scissors, cut virus-free seedling stem section (being with a leaf), transfer in culture vessel, every box 25-30 stem section, neatly be inserted on medium, covers lid.In transferring to again between illumination cultivation, on culturing rack, cultivate until test-tube plantlet meets the requirements or ties the potato results;
3. the placement of culture vessel: on tissue culture frame, the neat itemize of culture vessel is put, spacing 4 ~ 8cm between bar and bar, can 2 layers even 3 layers put, greatly improved unit area utilization ratio.Approximately 2 months production cycle of the present utility model, can produce 5-6 time every year, greatly improved single box knot potato number and the ratio that is greater than the test tube potato of 50mg.Produce the test tube potato the utility model unit are culturing room's year and can reach 100,000-250,000, than prior art, improve 2-3 doubly.
Above content is in conjunction with concrete embodiment further detailed description of the utility model, can not assert that concrete enforcement of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field, without departing from the concept of the premise utility, can also make some simple deduction or replace, all should be considered as belonging to protection domain of the present utility model.

Claims (3)

1. a Novel potato tissue culture vessel, described potato tissue comprises potato test-tube plantlet or test tube potato, it is characterized in that: described container comprises a plastic cylinder (5), lid (6), and described plastic cylinder is transparent, up big and down small, and lid is transparent; This plastic cylinder is square, and the inwall turning is 1/6 circular arc; The upper lower edge along, lid (6) of plastic cylinder (5) all has the gomphosis groove cooperatively interacted, plastic cylinder (5) upper along, lid (6) thus lower edge between seal the zigzag path of docking formation gas circuit by gomphosis groove.
2. Novel potato tissue culture vessel as claimed in claim 1 is characterized in that: what described lid was used is non-screw type, and Dual-layer groove fit-type is tightly connected.
3. Novel potato tissue culture vessel as claimed in claim 1, is characterized in that: the reinforcement that has been provided with fixing and ventilative post effect in the inside and outside groove of described lid.
CN201320034829.4U 2013-01-23 2013-01-23 Novel potato tissue culture container Expired - Fee Related CN203378377U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201320034829.4U CN203378377U (en) 2013-01-23 2013-01-23 Novel potato tissue culture container

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201320034829.4U CN203378377U (en) 2013-01-23 2013-01-23 Novel potato tissue culture container

Publications (1)

Publication Number Publication Date
CN203378377U true CN203378377U (en) 2014-01-08

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105532474A (en) * 2016-01-28 2016-05-04 遵义医学院 Tissue culture device convenient to observe and measure plant characters
CN108834907A (en) * 2018-08-30 2018-11-20 黑龙江省农业科学院植物脱毒苗木研究所 A kind of appearance potato device of toxicity-removing white potato microtuber culture

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105532474A (en) * 2016-01-28 2016-05-04 遵义医学院 Tissue culture device convenient to observe and measure plant characters
CN105532474B (en) * 2016-01-28 2018-01-19 重庆强大巴郡知识产权服务有限公司 A kind of tissue culture device be easy to observe and measure tree characteristics
CN108834907A (en) * 2018-08-30 2018-11-20 黑龙江省农业科学院植物脱毒苗木研究所 A kind of appearance potato device of toxicity-removing white potato microtuber culture

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140108

Termination date: 20220123

CF01 Termination of patent right due to non-payment of annual fee