CN205093317U - Plant tissue surface disinfection's simple and easy device - Google Patents

Abstract

The utility model discloses a plant tissue surface disinfection's simple and easy device, including upper and lower end equal open -ended glass pipe (1), the back suit is tied up in outer container (2) in two piece at least glass pipe (1), the upper end opening of arbitrary glass pipe (1) is all sealed through rubber stopper (5), the back cover is all tied up through copper mesh (3) to the lower extreme opening of all glass pipe (1).

Description

A Simple Device for Surface Disinfection of Plant Tissue

technical field

The utility model relates to a disinfection device, in particular to a simple device for disinfection of plant tissue surfaces.

Background technique

Surface disinfection of plant tissue samples is an indispensable link in the scientific research process of isolation and identification of plant pathogenic bacteria, plant tissue culture and plant symbiotic culture. The surface disinfection procedure that is considered to be more effective at present is to first soak the cleaned sample in 75% ethanol for 1min, then soak it in 1%-2% sodium hypochlorite solution for 3-10min (depending on the type of sample), and finally Soak in 75% or 95% ethanol for 30 seconds, wash with sterile water for 3-5 times and place on the plate ("EtOH-NaClO-EtOH" method). The current general implementation method of this disinfection program is to carry out the experimental operation of surface disinfection through four beakers or four glass petri dishes.

The thoroughness of surface disinfection directly affects the quality of follow-up research. However, the current general surface disinfection method has a small amount of samples to be processed, and there are not many types of samples to be processed, and the operation method is not simple enough and takes a lot of time. At the same time, the entire surface disinfection process requires strict aseptic operation, and the whole process must be completed in an ultra-clean bench, which urgently needs to improve the safety, space utilization, and speed and convenience of the surface disinfection device.

Utility model content

The technical problem to be solved by the utility model is to provide a simple device for disinfecting the surface of plant tissues with a simple structure.

In order to solve the above technical problems, the utility model provides a simple device for surface disinfection of plants, which includes glass tubes with open upper and lower ends; at least two glass tubes are bundled and set in the outer container; Sealed with a rubber stopper; the openings at the lower ends of all the glass tubes are sealed with copper mesh.

As an improvement to the simple device for surface disinfection of plants described in the utility model, the number of said glass tubes is seven.

As a further improvement to the simple device for disinfecting plant surfaces described in the present invention, the inner diameter of the glass tube is 2.5 cm, the outer diameter is 3 cm, and the height is 16 cm.

As a further improvement to the simple device for disinfecting plant surfaces described in the present invention, the glass tubes are bound and fixed by two rubber bands.

As a further improvement to the simple device for disinfecting plant surfaces described in the present invention, the outer container has an inner diameter of 13 cm, an outer diameter of 14 cm, a height of 18 cm, and a capacity of 1.85 L.

As a further improvement to the simple device for disinfecting the plant surface described in the utility model, the diameter of the copper mesh is 15cm.

Compared with the traditional plant surface disinfection device, the advantages of the utility model mainly include the following points:

1. Seven kinds of samples can be placed in seven glass tubes, which not only greatly improves the efficiency of processing multiple different kinds of samples, but also increases the utilization rate of drugs required for surface disinfection;

2. The back cover with copper mesh can ensure that the liquid used in the disinfection process can well enter the glass tube and immerse the sample. At the same time, the glass tube can be easily lifted up and down to fully rinse the sample in the liquid, making the surface disinfection more thorough;

3. The bottom cover with copper mesh can also avoid the loss of samples caused by changing the liquid in the outer container (the traditional method is to fix it with tweezers, and some samples will inevitably be lost during the flushing of the disinfectant liquid);

4. Compared with the traditional surface disinfection method, the use of this device can greatly improve the space utilization rate;

5. The outer container of this device can better avoid potential safety hazards such as open flames caused by ethanol splashes, and is safe to operate;

6. This device can be used for but not limited to healthy and susceptible plant samples, and can also be used for surface disinfection of samples of other tissues such as large fungal fruiting bodies, and has a wide range of applications;

7. The device is easy to operate, the constituent materials are easy to obtain, the cost is low, and the reproducibility is strong.

Description of drawings

Below in conjunction with accompanying drawing, the specific embodiment of the present utility model is described in further detail.

Fig. 1-1 is the main structural representation of the utility model;

Fig. 1-2 is the detailed structural representation of glass tube 1 in accompanying drawing 1-1;

Figure 1-3 is an enlarged schematic view of the mesh structure of the copper mesh 3 in the accompanying drawing 1-1;

Figure 2 is a schematic top view of Figure 1-1;

Figure 3-1 is a schematic diagram of actual use;

Figure 3-2 is another schematic diagram in actual use;

Figure 3-3 is another schematic diagram in actual use;

Fig. 4 is a schematic diagram of the steps of the existing traditional method.

detailed description

Embodiment 1, Fig. 1-1, 1-2, 1-3, 2～Fig. 3-3 have provided a kind of simple device of plant surface disinfection.

The simple device for disinfection of the plant surface comprises seven glass tubes 1, which are glass tubes whose upper and lower ports are not sealed, and the seven glass tubes 1 are bundled and fixed by two rubber bands 4; and any glass tube 1 The upper openings of the glass tubes are sealed correspondingly by rubber stoppers 5, and the lower openings of all glass tubes 1 bundled by rubber bands 4 are uniformly bundled and backed by a piece of copper mesh 3. After the seven glass tubes 1 are bound and fixed into a bundle, they are put into the outer container 2 .

Each glass tube 1 adopts an inner diameter of 2.5cm, an outer diameter of 3cm, and a height of 16cm; and the outer container 2 adopts an inner diameter of 13cm, an outer diameter of 14cm, a height of 18cm, and a capacity of 1.85L; and the copper mesh 3 is circular, and its diameter is 20cm. The number of mesh is 20 mesh.

Because the outer diameter of glass tube 1 is 3cm, after all glass tubes 1 are bundled, its diameter maximum dimension as shown in Figure 2 is 9cm, and it can be accommodated in the outer container 2 of its inner diameter 13cm, and leaves slit; And The diameter of the copper mesh 3 is 15cm. After wrapping the glass tube bundle with a maximum diameter of 9cm from the bottom, a part of the curling can be reserved to bind the glass tube bundle.

What the size of above glass tube 1 adopts is the comparatively general size in the prior art, and utilization rate is higher, and it is easy to buy in the market; And because the size of glass tube 1, the outer diameter size of its each glass tube 1 has 3cm, then its After forming a bundle with seven arrays, in its structure, any angle is the size of three arrays, and the diameter of the three arrays is 9 cm, which is the most suitable size while keeping as many glass tubes 1 as possible. The size for one-handed grasping (in the actual use process, there are more frequent operations such as hand-carrying glass tube bundles); and the size of the outer container 2 forms a gap with the size formed after the seven glass tubes 1 are assembled. It is convenient to put a solution (ethanol, sodium hypochlorite, sterile water, etc.) in the outer container 2 . The large-sized copper mesh 3 can bind the bottoms of the seven glass tubes 1 at one time and set the back cover, which can reduce the operation of the copper mesh 3 for back sealing. operate alone.

The actual operation mode of the utility model device has the following three kinds:

1. Prepare the operation process (as shown in Figure 3-1) of four outer containers 2 of the utility model device:

(1) Clean the samples with sterile water, sort them and trim them, and put the samples into the glass tube 1 with tweezers (a set of devices can put one or less than seven kinds of plant samples), and the rubber tube corresponding to the glass tube 1 Affix a label with the sample name or serial number on the plug 5, and fix the seven glass tubes 1 with rubber bands 4;

(2) Add 75% ethanol, 2% sodium hypochlorite, 90% ethanol and sterile water to the four outer containers 2 respectively (the amount added is based on the sample that has not passed through the glass tube 1);

(3) Hold the glass tube bundle (seven glass tubes 1), and immerse it in 75% ethanol for 30-60 seconds, during which time, lift and put down the glass tube bundle 2-3 times by hand;

(4) Transfer the glass tube bundle to the outer container 2 filled with 2% sodium hypochlorite, soak for 6-10 minutes, during which the glass tube bundle is lifted and put down several times by hand;

(5) Transfer the glass tube bundle to the outer container 2 filled with 90% ethanol, soak for 30-60 seconds, during which time, lift and put down the glass tube bundle 2-3 times by hand;

⑹Transfer the glass tube bundle to the outer container 2 filled with sterile water, replace the sterile water three times, take out the glass tube bundle and place the glass tube bundle on sterile filter paper to control the excess water.

2. Prepare a set of operation process of the utility model device (as shown in Figure 3-2):

(1) Clean the samples with sterile water, sort them and trim them, and put the samples into the glass tube 1 with tweezers (a set of devices can put one or less than seven kinds of plant samples), and the rubber tube corresponding to the glass tube 1 Affix a label with the sample name or serial number on the plug 5, and fix the seven glass tubes 1 with rubber bands 4;

(2) Add 75% ethanol to the outer container 2 (the amount added is subject to the sample that has not passed through the glass tube 1), and immerse the glass tube bundle in 75% ethanol for 30-60 seconds, during which time the glass tube bundle is lifted and put down by hand for 2 ~3 times;

(3) Replace the liquid in the outer container 2 with 2% sodium hypochlorite (the amount to be added is based on the sample submerged in the glass tube 1), and soak the glass tube bundle in the outer container 2 for 6-10 minutes. Second-rate;

(4) Replace the liquid in the outer container 2 with 90% ethanol (the amount added is subject to the sample that has not passed through the glass tube 1), and soak the glass tube bundle in the outer container 2 for 6-10 minutes. Second-rate;

(5) Replace the liquid in the outer container 2 with sterile water (the amount added is based on the sample that has not passed through the glass tube 1), replace the sterile water three times, take out the glass tube bundle, and place the glass tube bundle on sterile filter paper to control dryness. Excess moisture.

3. Prepare four sets of operating procedures of the utility model device (as shown in Figure 3-3):

(1) Clean the samples with sterile water, sort them and trim them, and put the samples into the glass tube 1 with tweezers (a set of devices can put one or less than seven kinds of plant samples), and the rubber tube corresponding to the glass tube 1 Affix a label with the name or serial number of the sample on the plug 5, and fix the glass tubes 1 in groups of seven with rubber bands 4;

(2) Add 75% ethanol to the four outer containers 2 (the amount added is subject to the sample that has not passed through the glass tube 1), and immerse the four groups of glass tube bundles in 75% ethanol for 30-60 seconds. Lift and lower each group of glass tube bundles 2 to 3 times;

(3) Replace the liquid in the four outer containers 2 with 2% sodium hypochlorite (the amount added is based on the sample that has not passed through the glass tube 1), and soak the glass tube bundles in the outer container 2 for 6-10 minutes. Lift and lower the glass tube bundle several times;

(4) Replace the liquid in the four outer containers 2 with 90% ethanol (the amount added is based on the sample that has not passed through the glass tube 1), and immerse the four groups of glass tube bundles in 90% ethanol for 30-60 seconds. Lift and lower each group of glass tube bundles 2 to 3 times by hand;

(5) Replace the liquid in the four outer containers 2 with sterile water (the amount added is based on the sample that has not passed through the glass tube 1), replace the sterile water three times, take out the glass tube bundle and put the glass tube bundle on sterile filter paper Control dry excess moisture.

In order to further illustrate the effect of this device, list the traditional method (see Figure 4) and use this device to carry out the comparison table of the time used for surface disinfection as follows:

Comparison table of time taken to disinfect surfaces using conventional methods and using the device

* Note: 1. The amount of each sample is calculated according to the maximum capacity that the utility model device can hold;

2. The operation mode three is based on the saturation capacity of four sets of the utility model device, that is, the processed sample volume is four times that of other modes.

Finally, it should also be noted that what is listed above is only a specific embodiment of the present invention. Apparently, the utility model is not limited to the above embodiments, and many variations are possible. All deformations that a person of ordinary skill in the art can derive or associate directly from the content disclosed in the utility model shall be considered as the protection scope of the utility model.

Claims (6)

1. A simple device for disinfecting the surface of plant tissue, comprising a glass tube (1) with upper and lower ends open; it is characterized in that: at least two glass tubes (1) are bundled and set in the outer container (2); The upper opening of any glass tube (1) is sealed by a rubber stopper (5); The openings at the lower ends of all the glass tubes (1) are bound and sealed with copper mesh (3). 2. The simple device for surface disinfection of plant tissue according to claim 1, characterized in that: the number of said glass tubes (1) is seven. 3. The simple device for surface disinfection of plant tissue according to claim 2, characterized in that: the inner diameter of the glass tube (1) is 2.5 cm, the outer diameter is 3 cm, and the height is 16 cm. 4. The simple device for surface disinfection of plant tissue according to claim 3, characterized in that: said glass tubes (1) are bound and fixed by two rubber bands (4). 5. The simple device for surface disinfection of plant tissue according to claim 1 or 4, characterized in that: the outer container (2) has an inner diameter of 13 cm, an outer diameter of 14 cm, a height of 18 cm, and a capacity of 1.85 L. 6. The simple device for surface disinfection of plant tissue according to claim 5, characterized in that: the copper mesh (3) has a diameter of 15 cm.