CN102018969B - Device for disinfecting surfaces of explants of plants - Google Patents
Device for disinfecting surfaces of explants of plants Download PDFInfo
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- CN102018969B CN102018969B CN 201010564702 CN201010564702A CN102018969B CN 102018969 B CN102018969 B CN 102018969B CN 201010564702 CN201010564702 CN 201010564702 CN 201010564702 A CN201010564702 A CN 201010564702A CN 102018969 B CN102018969 B CN 102018969B
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Abstract
The invention discloses a device for disinfecting the surfaces of explants of plants. The device comprises liquid storage bottles, a chlorine generator, a hydrochloric acid dripping pipe, a gas storage bottle and a disinfection bottle, wherein the first liquid storage bottle is connected with the chlorine generator, the output end of the hydrochloric acid dripping pipe is connected with the chlorine generator, the output end of the chlorine generator is connected with the disinfection bottle, the bottom of the disinfection bottle is connected with the input end of the gas storage bottle, the output end of the gas storage bottle is connected with the second liquid storage bottle, and the bottom of the first liquid storage bottle and the bottom of the second liquid storage bottle are communicated. The device provided by the invention is airtight so as to ensure the chlorine gas not to escape from the device; the device can be used repeatedly after the chlorine gas is generated once, thereby saving the cost; and the amount of the chlorine gas in the device remains unchanged, and the proper time for disinfecting different plant materials can be determined through tests, thereby facilitating accurately quantifying the disinfection conditions. The device provided by the invention is safe, environment-friendly, simple and effective, and is convenient to operate, low in construction cost, and suitable for the surface disinfection on the explants of most of plants, therefore, the device provided by the invention has great application value.
Description
Technical field
What the present invention relates to is a kind of device of biological technical field, specifically is a kind of plant explants surface sterilization device.
Background technology
Plant tissue culture technique is the important basic fundamental of plant cell engineering, hereditism, plant physiology, Biochemistry and Molecular Biology research.Plant tissue culture technique not only is used for breeding fast, at aspects such as haploid breeding, germplasm preservation, Physiologic Studies and gene transformation purposes is widely arranged also.All the time running through an aseptic concept in plant tissue culture, a plant explants must could obtain sterilizable material by surface sterilization earlier then before inoculation.
Traditional approach that is used for sterilization before the plant explants inoculation mainly is with one or more antiseptic solutions immersions, and then with the residual poisonous ion of sterilized water flush away explant.The shortcoming of this method is: 1. complex operation; 2. after toxic component is penetrated into plant tissue inside, follow-up cultivation is caused adverse effect; 3. used poisonous waste liquor contamination environment.
Disinfection by chlorine is a kind of effective explant sterilization method of inventing in recent years, and Disinfection Effect is good, and is easy to operate.Find through the retrieval to prior art, bang is female, bright 2002 of Wei Zhi is in " a kind of sterilization method of Semen sojae atricolor mature seed " literary composition that " Plant Physiology Communications " delivered, adopt chlorination plant as shown in Figure 1, Disinfection Effect is more satisfactory, but this device is revealed chlorine around in the environment easily, and life-time service can cause chronic poisoning, and weight person is disabled.
Summary of the invention
The present invention is directed to the prior art above shortcomings, a kind of plant explants surface sterilization device is provided, by liquid pump chlorine is come and gone in the container of gas bomb and Sheng sterile material and shift, thereby reach the purpose of surface sterilization.
The present invention is achieved by the following technical solutions, the present invention includes: several liquid containing bottles, chlorine still, hydrochloric acid splash into pipe, gas bomb and sterilization bottle, wherein: first liquid containing bottle is connected with chlorine still, the outfan that hydrochloric acid splashes into pipe links to each other with chlorine still, the outfan of chlorine still links to each other with the sterilization bottle, the bottom of sterilization bottle links to each other with the input of gas bomb, and the outfan of gas bomb is connected with second liquid containing bottle, and the bottom of first liquid containing bottle and second liquid containing bottle is connected.
Described chlorine still is provided with the sodium hypochlorite entrance, and described hydrochloric acid splashes into pipe and is provided with the fast switch of control and volume markings, and the bottom that this hydrochloric acid splashes into pipe contacted with the bottle end of chlorine still.
The volume of described first liquid containing bottle and second liquid containing bottle equates that the volume of described sterilization bottle and gas bomb equates that the volume of described liquid containing bottle is the twice of gas bomb volume.
Be provided with liquid pump between described first liquid containing bottle and second liquid containing bottle, this liquid pump is the common liq pump that has two-way switch.
Between described chlorine still and the sterilization bottle, be equipped with the switch folder between sterilization bottle and the gas bomb.
When the present invention works, this device is connected place on the superclean bench earlier, material subject to sterilization is placed in the sterile material storing bottle, and the lid of switch folder and second liquid containing bottle is taken off, and fills second liquid containing bottle with saturated nacl aqueous solution.The rapid sodium hypochlorite of adding certain volume in the chlorine still adds the concentrated hydrochloric acid of certain volume then in proportion.Order adds concentrated hydrochloric acid again for adding sodium hypochlorite earlier.Add and seal rapidly after good, open liquid pump simultaneously to Peak Flow Rate, the liquid of half volume in second liquid containing bottle was transferred in the liquid containing bottle within 1 minute, thereby made the chlorine that generates transfer in the sterile material storing bottle.Close liquid pump at last, the switch folder picks up counting on the folder.
After sterilization finishes, open the switch folder.Open liquid pump, a remaining semiliquid in second liquid containing bottle all is extracted in first liquid containing bottle, thereby chlorine is all transferred in the gas bomb.The switch folder takes off material bottle subject to sterilization on the folder, and sterilization is finished.
Do not need regeneration chlorine when using next time, as long as liquid pump is reached opposite direction, makes that the chlorine in the gas bomb is transferred to material bottle subject to sterilization, chlorine transferred in the chlorine still after sterilization finishes to get final product again.
Compare with existing device, apparatus of the present invention are airtight, guarantee that chlorine does not leak; Can reuse after once generating chlorine, save cost; Because the amount of chlorine remains unchanged in the device, different vegetable materials can be determined suitable disinfecting time by test, is convenient to conditions for sterilization is accurately quantized.
Description of drawings
Fig. 1 is the prior art structural representation.
Fig. 2 is structural representation of the present invention.
The specific embodiment
Below embodiments of the invention are elaborated, present embodiment is being to implement under the prerequisite with the technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
As shown in Figure 2, present embodiment comprises: first liquid containing bottle 1, chlorine still 2, hydrochloric acid splash into pipe 3, gas bomb 4, sterilization bottle 5 and second liquid containing bottle 6, wherein: first liquid containing bottle 1 is connected with chlorine still 2, the outfan that hydrochloric acid splashes into pipe 3 links to each other with chlorine still 2, the outfan of chlorine still 2 links to each other with sterilization bottle 5, the bottom of sterilization bottle 5 links to each other with the input of gas bomb 4, the outfan of gas bomb 4 is connected with second liquid containing bottle 6, and the bottom of first liquid containing bottle 1 and second liquid containing bottle 6 is connected.
Described chlorine still 2 is provided with sodium hypochlorite entrance 7, and described hydrochloric acid splashes into pipe 3 and is provided with the fast switch 8 of control and volume markings, and the bottom that this hydrochloric acid splashes into pipe 3 contacted with the bottle end of chlorine still 2.
The volume of described first liquid containing bottle 1 and second liquid containing bottle 6 equates that the volume of described sterilization bottle 5 and gas bomb 4 equates that the volume of described liquid containing bottle is the twice of gas bomb 4 volumes.
Be provided with liquid pump 9 between described first liquid containing bottle 1 and second liquid containing bottle 6, this liquid pump 9 is for having the common liq pump of two-way switch.
Between described chlorine still 2 and the sterilization bottle 5, be equipped with switch between sterilization bottle 5 and the gas bomb 4 and press from both sides.
Present embodiment is inoculated the front surface sterilization with chlorination plant to soybean seed, and the volume that first liquid containing bottle 1 and second liquid containing bottle 6 are set is 300 milliliters, and chlorine still 2 is 150 milliliters with the volume of gas bomb 4.100 of Semen sojae atricolor subject to sterilization.Institute adds 75 milliliters of new uncork liquor natrii hypochloritises, 25 milliliters of concentrated hydrochloric acid.Sterilization time is 1 hour.Sterilization is all inoculated the MS solid medium after finishing, and none example is polluted, and germination percentage reaches 100%.
The device that uses in the present embodiment is applicable to the explant inoculation front surface sterilization of most of plant, has very big using value.
Claims (7)
1. plant explants surface sterilization device, it is characterized in that, comprise: liquid containing bottle, chlorine still, hydrochloric acid splash into pipe, gas bomb and sterilization bottle, wherein: first liquid containing bottle is connected with chlorine still, the outfan that hydrochloric acid splashes into pipe links to each other with chlorine still, and the outfan of chlorine still links to each other with the sterilization bottle, and the bottom of sterilization bottle links to each other with the input of gas bomb, the outfan of gas bomb is connected with second liquid containing bottle, and the bottom of first liquid containing bottle and second liquid containing bottle is connected; Be provided with liquid pump between described first liquid containing bottle and second liquid containing bottle, this liquid pump is the common liq pump that has two-way switch.
2. plant explants surface sterilization device according to claim 1 is characterized in that described chlorine still is provided with the sodium hypochlorite entrance.
3. plant explants surface sterilization device according to claim 1 is characterized in that, described hydrochloric acid splashes into pipe and is provided with the fast switch of control and volume markings, and the bottom that this hydrochloric acid splashes into pipe contacted with the bottle end of chlorine still.
4. plant explants surface sterilization device according to claim 1 is characterized in that, the volume of described first liquid containing bottle and second liquid containing bottle equates.
5. plant explants surface sterilization device according to claim 1 is characterized in that, the volume of described sterilization bottle and gas bomb equates.
6. plant explants surface sterilization device according to claim 1 is characterized in that, the volume of described liquid containing bottle is the twice of gas bomb volume.
7. plant explants surface sterilization device according to claim 1 is characterized in that, between described chlorine still and the sterilization bottle, be equipped with the switch folder between sterilization bottle and the gas bomb.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010564702 CN102018969B (en) | 2010-11-30 | 2010-11-30 | Device for disinfecting surfaces of explants of plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010564702 CN102018969B (en) | 2010-11-30 | 2010-11-30 | Device for disinfecting surfaces of explants of plants |
Publications (2)
| Publication Number | Publication Date |
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| CN102018969A CN102018969A (en) | 2011-04-20 |
| CN102018969B true CN102018969B (en) | 2013-07-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010564702 Expired - Fee Related CN102018969B (en) | 2010-11-30 | 2010-11-30 | Device for disinfecting surfaces of explants of plants |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293155B (en) * | 2011-07-20 | 2013-10-30 | 天津大学 | Convenient explant sterilization method by cup method |
| CN104026013B (en) * | 2014-06-17 | 2015-07-08 | 西北农林科技大学 | Sterile disinfection device for surfaces of plant tissues |
| CN104737910A (en) * | 2015-03-06 | 2015-07-01 | 中南民族大学 | Easy seed sterilization method and device |
| CN106119076A (en) * | 2016-06-29 | 2016-11-16 | 无锡南理工科技发展有限公司 | A kind of crops training spore suspension preparation facilities and method |
| CN108552060A (en) * | 2018-07-19 | 2018-09-21 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | The sterilizing methods of sweet potato tissue cultures explant |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201131939Y (en) * | 2007-12-19 | 2008-10-15 | 吕延利 | Environmental protection micro epoxyethane sterilizing chambers for medical purpose |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5993864A (en) * | 1997-07-11 | 1999-11-30 | Kross; Robert D. | Chlorine dioxide chelate compositions and method of use |
| JP2004215930A (en) * | 2003-01-15 | 2004-08-05 | Kitano Takahiro | Method and device for sterilizing and cleaning endoscope |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201131939Y (en) * | 2007-12-19 | 2008-10-15 | 吕延利 | Environmental protection micro epoxyethane sterilizing chambers for medical purpose |
Non-Patent Citations (4)
| Title |
|---|
| JP特开2004-215930A 2004.08.05 |
| 刘海坤，卫志明.一种大豆成熟种子的消毒方法.《植物生理学通讯》.2002,第38卷(第3期),第260-261页. * |
| 循环概念在有毒气体实验中的运用;黄无违;《实验教学与仪器》;20030630;第20卷(第6期);第25页左栏第1-20行、图1-2 * |
| 黄无违.循环概念在有毒气体实验中的运用.《实验教学与仪器》.2003,第20卷(第6期), |
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