CN106242341A - Ternary microorganism renovation agent, preparation method and applications for repairing concrete crack - Google Patents
Ternary microorganism renovation agent, preparation method and applications for repairing concrete crack Download PDFInfo
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- CN106242341A CN106242341A CN201610552775.9A CN201610552775A CN106242341A CN 106242341 A CN106242341 A CN 106242341A CN 201610552775 A CN201610552775 A CN 201610552775A CN 106242341 A CN106242341 A CN 106242341A
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- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B40/00—Processes, in general, for influencing or modifying the properties of mortars, concrete or artificial stone compositions, e.g. their setting or hardening ability
- C04B40/0028—Aspects relating to the mixing step of the mortar preparation
- C04B40/0039—Premixtures of ingredients
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- C—CHEMISTRY; METALLURGY
- C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
- C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
- C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
- C04B2111/72—Repairing or restoring existing buildings or building materials
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Abstract
Present invention provide for the ternary microorganism renovation agent of repairing concrete crack, utilize oxygen release agent to meet the characteristic of water reaction releasing oxygen, slowly supplement the dissolved oxygen content in distress in concrete water environment, promote the aerobic respiration of microorganism, and then the synthesis of acceleration calcium carbonate, improve remediation efficiency.In addition, a kind of ternary microorganism renovation agent preparation method for repairing concrete crack that the present invention provides, carry out pulverizing, grinding by certain mass ratio by oxygen release agent, spore powder and nutrient substance, will grind after material mix homogeneously and carry out tabletting, be fabricated to microgranule, obtain ternary microorganism renovation agent, above-mentioned preparation method technique is simple, raw material sources are extensive, with low cost, and environmentally safe.
Description
Technical field
The present invention relates to concrete self-repair technology field, particularly relate to a kind of ternary for repairing concrete crack micro-
Bioremediation agents, preparation method and applications.
Background technology
Reinforced concrete structure is due to by physics, the comprehensive function of the factor such as chemical and biological, nearly all concrete
Building microcrack all can inevitably occur so that the harmful substances such as water, oxygen, carbon dioxide and chloride be easier to into
Enter, ultimately result in the corrosion of the bar construction of inside concrete so that overall structure is often not up to life expectancy and destroys, by
This causes the problems such as repairing, maintenance also to become increasingly conspicuous.The method of concrete reparation is broadly divided into chemical method, Physical and bioanalysis
Three major types.Traditional physics and chemical repair method can effectively repair microcrack to a certain extent, but still suffer from repair time
Length, poor effect, repair process are difficult to the problems such as regulation and control.
In recent years, distress in concrete is repaired and is increasingly becoming new study hotspot by the mineralization activity utilizing microorganism.
By resistance microorganism dormancy body (such as spore), fix or be coated on such as kieselguhr, leca, silica gel and microcapsule etc. and carry
Mixing concrete, once cracking initiation in body and together with nutrient substance, water and air enters crack-induced spore and sprouts formation
Trophocyte, active cellular metabolism utilizes nutrient substance produce carbon dioxide and form carbonate further, and carbonate is carefully
Calcium carbonate is formed with calcium ion under cellular surface or Extracellular polymers inducing action.
Crack repair function microorganism needs dissolved oxygen supply to meet Repiration, forms carbon dioxide or carbonate,
Form the material calcium carbonate in healing crack further.Owing in distress in concrete, dissolved oxygen content is the lowest, split at microorganism remediation
During seam, dissolved oxygen is consumed to very low level further.Belie etc. find the fracture faces that dissolved oxygen content is of a relatively high
When healing completely, the most not healing inside crack, the calcium carbonate content that crack is internally formed is far below fracture faces.So,
The supplementary of dissolved oxygen is to maintain bacterial remediation activity and the key promoting distress in concrete to repair.
Inventor is found by literature survey, up to now, there is no and utilizes oxygen release agent to promote the formation of bacteria-induction calcium carbonate
Report.
Summary of the invention
In consideration of it, be necessary to provide a kind of can the ternary microorganism renovation agent of effective repairing concrete crack.
For achieving the above object, the present invention uses following technical proposals:
The ternary microorganism renovation agent of a kind of distress in concrete, including following quality component: oxygen release agent 70%-95%, spore
Sub-powder 1%-10%, nutrient substance 5%-15%, acid-base modifier 1%-10%.
In certain embodiments, described oxygen release agent is at least one in peracid salt or metal peroxides.
In certain embodiments, described peracid salt is at least one in sodium peroxydisulfate or sodium carbonate peroxide, described
Metal peroxides is at least one in calper calcium peroxide or Magnesium dioxide.
In certain embodiments, described spore powder is the resting spore of Alkaliphilic bacillus.
In certain embodiments, the resting spore of described Alkaliphilic bacillus is Coriolis bacillus cereus, false strong spore bar
In bacterium, Bacillus sphaericus, Bacillus pasteurii, Bacillus sp.H4CGMCC 9629 at least one.
In certain embodiments, described nutrient substance be antibacterial may utilize and be translated into the various organic of calcium carbonate or
In inorganic carbon source at least one.
In certain embodiments, described organic or inorganic carbon source is yeast powder, peptone, sodium lactate, sodium citrate, paddy ammonia
Acid sodium, Sodium L-alaninate, Sodium Pyruvate, sodium propionate, sodium acetate, sodium formate, starch at least one.
In certain embodiments, the one of which during described acid-base modifier is lactic acid powder, citric acid powder.
It addition, present invention also offers the preparation side of a kind of ternary microorganism renovation agent for repairing concrete crack
Method, comprises the steps:
By oxygen release agent, spore powder, nutrient substance and acid-base modifier it is in mass ratio (70%-95%): (1%-10%):
(5%-15%): (1%-10%) pulverize, it is standby to grind；
Material mix homogeneously after grinding also carries out tabletting, and every time punching press adds the mixed-powder of 20-40mg, makes
Become microgranule, obtain described ternary microorganism renovation agent.
In certain embodiments, the particle diameter of grinding is 100 mesh, a diameter of 3-10mm of described microgranule, a height of 2-10mm.
Additionally, present invention also offers the application in repairing concrete crack of a kind of above-mentioned ternary microorganism renovation agent.
The present invention uses technique scheme to possess following beneficial effect:
The ternary microorganism renovation agent for repairing concrete crack that the present invention provides, utilizes oxygen release agent to meet water reaction and releases
Put the characteristic of oxygen, slowly supplement the dissolved oxygen content in distress in concrete water environment, promote the aerobic respiration of microorganism,
And then the synthesis of acceleration calcium carbonate, improve remediation efficiency.
The ternary microorganism renovation agent preparation method for repairing concrete crack that the present invention provides, by oxygen release agent, spore
Sub-powder and nutrient substance are carried out pulverizing, grinding by certain mass ratio, will grind after material mix homogeneously and carry out tabletting, make
Becoming microgranule, obtain ternary microorganism renovation agent, above-mentioned preparation method technique is simple, and raw material sources are extensive, with low cost, and to ring
Border is pollution-free.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage become apparent from, below in conjunction with embodiment, the present invention is carried out
Further describe.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to limit
The present invention.
A kind of ternary microorganism renovation agent for repairing concrete crack, including following quality component: oxygen release agent 70%-
95%, spore powder 1%-10%, nutrient substance 5%-15%, acid-base modifier 1%-10%.
Wherein, described oxygen release agent is at least one in peracid salt or metal peroxides.Preferably, described peracid salt
For in sodium peroxydisulfate or sodium carbonate peroxide at least one, described metal peroxides is in calper calcium peroxide or Magnesium dioxide
At least one.
Wherein, described spore powder is the resting spore of Alkaliphilic bacillus.Preferably, the dormancy of described Alkaliphilic bacillus
Spore is Coriolis bacillus cereus, false bacillus firmus, Bacillus sphaericus, Bacillus pasteurii, Bacillus
In sp.H4CGMCC 9629 at least one.
Wherein, during described nutrient substance is the various organic or inorganic carbon sources that antibacterial may utilize and be translated into calcium carbonate
At least one.Preferably, described organic or inorganic carbon source be yeast powder, peptone, sodium lactate, sodium citrate, sodium glutamate, third
At least one in propylhomoserin sodium, Sodium Pyruvate, sodium propionate, sodium acetate, sodium formate, starch.
Wherein, described acid-base modifier is acid organic or inorganic thing.Preferably, described organic or inorganic soda acid regulator
For at least one in lactic acid powder, citric acid powder.
It is appreciated that the ternary microorganism renovation agent for repairing concrete crack that the present invention provides, utilizes peroxy acid
The characteristic of water reaction releasing oxygen met by salt and peroxide, slowly supplements the dissolved oxygen content in distress in concrete water environment, promotees
Enter the aerobic respiration of microorganism, and then the synthesis of acceleration calcium carbonate, improve remediation efficiency.
It addition, present invention also offers the preparation side of a kind of ternary microorganism renovation agent for repairing concrete crack
Method, it comprises the steps:
By oxygen release agent, spore powder, nutrient substance and acid-base modifier it is in mass ratio (70%-95%): (1%-10%):
(5%-15%): (1%-10%) pulverize, it is standby to grind；Preferably, the particle diameter of grinding is 100 mesh.
Material mix homogeneously after grinding also carries out tabletting, and every time punching press adds the mixed-powder of 20-40mg, makes
Become microgranule, obtain described ternary microorganism renovation agent, it is preferable that a diameter of 3-10mm of described microgranule, a height of 2-10mm.
The ternary microorganism renovation agent preparation method for repairing concrete crack that the present invention provides, by oxygen release agent, spore
Sub-powder and nutrient substance are carried out pulverizing, grinding by certain mass ratio, will grind after material mix homogeneously and carry out tabletting, make
Becoming microgranule, obtain ternary microorganism renovation agent, above-mentioned preparation method technique is simple, and raw material sources are extensive, with low cost, and to ring
Border is pollution-free.
The ternary microorganism renovation agent that the present invention provides can be used for repairing concrete crack.
It is specific embodiment part below.
Prepared by embodiment 1-calper calcium peroxide-spore-sodium lactate ternary microorganism renovation agent
1) preparation of spore.Use the bacterial strain Bacillus disclosed in Shenzhen University patent application CN201410478149.0
Sp.H4, preserving number is CGMCC NO.9629.Bacterial strain H4 is rule with inoculating loop on alkalescence LB solid medium flat board, 30 DEG C
It is inverted and cultivates 16-20 hour.Choose planar surface colony inoculation with the inoculating loop of calcination sterilizing to contain in alkalescence LB culture fluid, culture fluid
In the triangular flask that volume is 250ml, every bottle of culture fluid volume is 50ml, inoculated triangular flask is placed in isothermal vibration and cultivates
In case, 30 DEG C, 150rpm cultivates 16-20 hour, draws this sending out containing a large amount of antibacterials of 8ml with 10ml micropipettor afterwards
Ferment liquid, adds MMN-1 and promotees sporulation culture fluid, and this MMN-1 culture fluid is contained in the triangular flask that volume is 500ml, every bottle of training
Nutrient solution volume is 100ml, is placed in isothermal vibration incubator by inoculated triangular flask, 30 DEG C, and 150rpm cultivates 5-7 days, it
After this fermentation liquid containing a large amount of spore is centrifuged, centrifugal rotational speed is 6000 g, and centrifugation time is 10 minutes, centrifugal
After topple over and give up supernatant, and in spore precipitate, add deionized water shake resuspended spore, recentrifuge, centrifugal rotational speed is 6000
G, centrifugation time is 10 minutes, repeats above operated wash spore 8-10 time.Last spore precipitation use is cleaned
Spoon takes out, and is laid in the glass culture dish of diameter 15cm, and the culture dish filling spore precipitation is put into-80 DEG C of refrigerator 2-4
Hour, until spore precipitation is frozen as solid, then this culture dish is put into freezer dryer carries out lyophilizing, temperature-55 DEG C, time
Between 48-96 hour, until spore precipitation become powdered.Spore dry powder is contained in blue lid wide-mouth screw socket bottle, this bottle is placed in
In the PC-3 plastic vacuum desiccator of Shanghai Yue Ci company, extraction valve is opened, is connected with vacuum pump, evacuation, until very
Empty meter reading reaches-0.1Mpa, closes extraction valve, this vacuum desiccator is placed in room temperature preservation standby.
Composition and the preparation method of alkalescence LB solid medium used by the present embodiment are as follows: yeast powder 5g/L, tryptone
10g/L, sodium carbonate 5.3g/L, sodium bicarbonate 4.2g/L, agar powder 15g/L；Yeast powder, tryptone is weighed by concentrations above
It is dissolved in 270ml water with agar powder；Weigh sodium carbonate and sodium bicarbonate is dissolved in 30ml water；In nothing after two kinds of solution sterilizations
Mixing while hot in bacterium superclean bench, and pour in the disposable sterilized plastic culture dish of diameter 9cm, each culture dish 20ml is left
The right side, cools down 10-20 minute, treats culture medium solidifying.
Composition and the preparation method of alkalescence LB culture fluid used by the present embodiment are as follows: yeast powder 5g/L, tryptone 10g/
L, sodium carbonate 5.3g/L, sodium bicarbonate 4.2g/L；Weigh yeast powder by concentrations above, tryptone is dissolved in 900ml water；Claim
Amount sodium carbonate is dissolved in 100ml water；Mix in aseptic superclean bench after two kinds of solution sterilizations, be sub-packed in sterilizing
In 250ml triangular flask, every bottle of 50ml.
Used by the present embodiment, MMN nutrient media components and preparation method are as follows, starch 1g/L, yeast powder 3g/L, potassium nitrate
3.75mM, ammonium chloride 3.7mM, potassium dihydrogen phosphate 0.15mM, calcium chloride 1.53mM, potassium chloride 2.68mM, magnesium chloride 1mM, lactic acid
Sodium 10mM, sodium carbonate 50mM, sodium bicarbonate 50mM.By concentrations above weigh sodium carbonate and sodium bicarbonate be dissolved in 100ml go from
In sub-water, it is sub-packed in 15ml centrifuge tube, often pipe 10ml；Weigh other composition to be dissolved in 900ml deionized water, be sub-packed in
In 500ml triangular flask, every bottle of 90ml；After sterilizing, fetching has the centrifuge tube of the soda-sodium bicarbonate solution of 10ml, by solution
Pour in the above-mentioned triangular flask filling 90ml culture fluid, each triangular flask add 1 centrifuge tube soda-sodium bicarbonate solution,
Mix.
2) preparation of ternary microorganism preparation for repairing.Take peroxidating calcium powder, lactic acid powder, sodium lactate powder and spore dry powder respectively
With mortar grinder, sieving with the screen cloth of 100 mesh, the powder that collection leaks through mesh is standby.The peroxide that will sieve with 9:1 mass ratio
Change calcium powder and lactic acid powder mixing and stirring, referred to as oxygen release agent powder.Die is placed on pad mould on, then weigh oxygen release agent powder 20mg,
Sodium lactate powder 15mg and spore dry powder 2mg is sequentially added in the hole of tablet machine die, the die and pad mould that fill powder is placed on
On tablet machine die shoe, firmly pressing tablet machine lever makes drift enter in the hole of die, is compacted by powder and in blocks in a mold,
Taking off die, be positioned over upward on die shoe by pad mould upset reverse side, die upward, then is put in the hole of pad mould by the hole at the pad mould back side
On, firmly pressing tablet machine lever makes drift enter in the hole of die again, is released by sheet, and sheet falls in the hole of pad mould, by sheet
Being put in dry brown centrifuge tube, be put in 4 DEG C of Refrigerator stores standby, this sheet is referred to as " calper calcium peroxide-spore-sodium lactate " sheet.
According to above operation, the sheet prepared under the conditions of being not added with calcium peroxide powder is referred to as " spore-sodium lactate " sheet.
3) cleaning of teat glass.Selection internal diameter is 7mm, and external diameter is 8.5mm, and the teat glass of a length of 12cm is as inspection
Survey the culture vessel of ternary microorganism preparation for repairing, for detecting the ability of said preparation synthetic calcium carbonate.Teat glass is placed in
Soaked overnight in 2% dilute hydrochloric acid, rinses respectively 4 times and 2 times with tap water, deionized water the most successively, is placed in by teat glass
Blowing-type drying baker is dried；Will dry after teat glass be immersed in soaked overnight in 95% ethanol, after rush with deionized water
Wash 4 times；Teat glass is immersed in soaked overnight in hydrogen peroxide/concentrated nitric acid washing liquid, this washing liquid be water, 30% hydrogen peroxide, 65%
Concentrated nitric acid is with volume ratio 1:1:1 mix homogeneously；By teat glass take out, rinse respectively 4 times with tap water, deionized water successively and
2 times.With high-pressure steam sterilizing pan in 121 DEG C of sterilizing 20min, standby.
4) detection of insoluble calcium conversion capability.Taking 60 above-mentioned teat glasses, often pipe adds 2ml sterilized CPM training
Nutrient solution；Take wherein 30 by all means, add 1 " calper calcium peroxide-spore-sodium lactate " sheet the most by all means；Other 30 by all means, add the most by all means
1 " spore-sodium lactate " sheet；In above 60 teat glasses, add sterilizing paraffin oil 200 microlitre, teat glass is placed in
In the hole of 96 deep-well plates, keep vertically placing and putting in constant incubator, cultivate 30 days for 30 DEG C.
Wherein, take the most respectively and add the teat glass 3 of " calper calcium peroxide-spore-sodium lactate " sheet and add " bud
Spore-sodium lactate " teat glass 3 of sheet, then take one with the 2ml syringe of the supporting syringe needle of standard, insert the needle into glass
In test tube below paraffin oil liquid level, pull syringe piston exhaustion paraffin oil, then the culture fluid in teat glass is poured out.With
The syringe needle of 20cm length inserts bottom teat glass, is smashed to pieces by the calper calcium peroxide sticking to bottom, then rushes with deionized water
Wash teat glass inwall 4-6 time, removed the calper calcium peroxide and the CPM culture fluid composition of residual smashed to pieces by washing.Try to glass
Adding 2ml concentration in pipe is the dilute hydrochloric acid of 1M, and dilute hydrochloric acid forms solubility calcium with the calcium carbonate reaction of formation on glass tubing inwall
Ion.Seal to glass tubing with preservative film, stand 1-2 hour, then pour the dilute hydrochloric acid solution in glass tubing into 2ml centrifuge tube
In, cover tightly lid, repeatedly overturn centrifuge tube, mix standby.
Dilute hydrochloric acid solution in above-mentioned 2ml centrifuge tube is diluted 2-20 times, inhales with the multichannel liquid-transfering gun that range is 10 μ L
Take 3.75 μ L of supernatant liquid and add in another new clear microplate, need to adding with the multichannel liquid-transfering gun that range is 300 μ L
The hole of test sample product adds 300 μ L calcium ion colour developing application liquid, is blank, lucifuge reaction 5-with 1M hydrochloric acid solution
15min.After reaction terminates, microplate reader is used to measure the absorbance at 575nm of chromogenic reaction liquid, according to standard curve and dilution
Multiple calculates the content of insoluble calcium in different glass pipe.
Experiment finds, after cultivating 30 days, adds shape on the teat glass inwall of 1 " calper calcium peroxide-spore-sodium lactate " sheet
Become 2.03mg insoluble calcium, and added that only to form 0.39mg on the teat glass inwall of 1 " spore-sodium lactate " sheet insoluble
Calcium.The oxygen that calper calcium peroxide and water slow reaction discharge promotes the mineralization of antibacterial, defines more insoluble calcium.
The preparation of above-mentioned colour developing application liquid: before use, how many according to institute's expense, by o-cresol phthalein complexon (OCPC)
Developer mixes with 2-amino-2-methyl-1-propanol (AMP) buffer equal-volume.After mixing, draw 300 μ L colour developing application liquid
Add in clear microplate hole, detect its absorbance value to wavelength 575nm light with microplate reader detection, if OD575 0.3 with
Under, then it represents that this solution is qualified, it is possible to use.
The preparation of above-mentioned o-cresol phthalein complexon (OCPC) developer: weigh 8-hydroxyquinoline 500mg and put in beaker, add
Concentrated hydrochloric acid 5000 μ L so that it is dissolve and proceed in 500ml volumetric flask, adds o-cresol phthalein complexon 25mg, waits to be completely dissolved
After, add 1mL Triton X-100 (TritonX-100) mixing, then add deionized water to 500ml, put polyethylene bottle
Interior 4 DEG C of preservations.
The preparation of above-mentioned 2-amino-2-methyl-1-propanol (AMP) alkaline buffer: put deionized water in 1L volumetric flask
500mL, adds AMP 89.14g, adds water to 1L until completely dissolved, put 4 DEG C of preservations in polyethylene bottle.
The making of standard curve: concentrate in 100mL volumetric flask constant volume dilution calcium atom spectrum analysis standard with 1M dilute hydrochloric acid
Liquid (calcium atom spectrum analysis standard concentrated solution 1.00g Ca, sigma Products) to after 10g/L (250mM) subpackage in-20 DEG C
Save backup.With 1M hydrochloric acid solution prepare gradient concentration ionic calcium soln: 0.25mM, 0.5mM, 1mM, 2mM, 3mM, 4mM, 5mM.
Drawing 3.75 μ L each concentration ionic calcium soln with the multichannel liquid-transfering gun of 10 μ L to add in clear microplate, each concentration adds 3
Hole, adds 300 μ L calcium ion colour developing application with the multichannel liquid-transfering gun of 300 μ L in the hole added with gradient concentration ionic calcium soln
Liquid mixes, and is blank, lucifuge reaction 5-15min with 1M hydrochloric acid solution.After reaction terminates, microplate reader is used to measure colour developing
The absorbance at 575nm of reactant liquor, with calcium ion concentration as abscissa, OD575 value is vertical coordinate, draws standard curve, mark
The binary fit equation of directrix curve is: y=0.1009x-0.0006, R2=0.9992.
Embodiment 2 Magnesium dioxides-spore-sodium lactate ternary microorganism renovation agent has the mineralization ability of excellence
Operational approach, only need to be by with the peroxidating calcium powder in peroxidating magnesium powder alternate embodiment 1 with embodiment 1.Experiment is sent out
Existing, after cultivating 30 days, add that to define 1.28mg on the teat glass inwall of 1 " Magnesium dioxide-spore-sodium lactate " sheet insoluble
Property calcium, and add and only form 0.37mg insoluble calcium on the teat glass inwall of 1 " spore-sodium lactate " sheet.Magnesium dioxide with
The oxygen of water slow reaction release promotes the mineralization of antibacterial, defines more insoluble calcium.
Embodiment 3 SODIUM PERCARBONATE-spore-sodium lactate ternary microorganism renovation agent has the mineralization ability of excellence
Operational approach, only need to be by with the peroxidating calcium powder in SODIUM PERCARBONATE powder alternate embodiment 1 with embodiment 1.Experiment is sent out
Existing, after cultivating 30 days, add that to define 1.21mg on the teat glass inwall of 1 " SODIUM PERCARBONATE-spore-sodium lactate " sheet insoluble
Property calcium, and add and only form 0.39mg insoluble calcium on the teat glass inwall of 1 " spore-sodium lactate " sheet.SODIUM PERCARBONATE with
The oxygen of water slow reaction release promotes the mineralization of antibacterial, defines more insoluble calcium.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (11)
1. the ternary microorganism renovation agent for repairing concrete crack, it is characterised in that include following quality component: release
Oxygen agent 70%-95%, spore powder 1%-10%, nutrient substance 5%-15%, acid-base modifier 1%-10%.
2. the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 1, it is characterised in that described in release
Oxygen agent is at least one in peracid salt or metal peroxides.
3. the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 2, it is characterised in that described mistake
Oxygen hydrochlorate is at least one in sodium peroxydisulfate or sodium carbonate peroxide, and described metal peroxides is calper calcium peroxide or peroxidating
At least one in magnesium.
4. the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 1, it is characterised in that described spore
Sub-powder is the resting spore of Alkaliphilic bacillus.
5. the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 4, it is characterised in that described addicted to
The resting spore of alkali bacillus cereus be Coriolis bacillus cereus, false bacillus firmus, Bacillus sphaericus, Bacillus pasteurii,
In Bacillus sp.H4CGMCC 9629 at least one.
6. the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 1, it is characterised in that described battalion
Foster material is that antibacterial may utilize and be translated at least one in the various organic or inorganic carbon sources of calcium carbonate.
7. the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 6, it is characterised in that described in have
Machine or inorganic carbon source are yeast powder, peptone, sodium lactate, sodium citrate, sodium glutamate, Sodium L-alaninate, Sodium Pyruvate, propanoic acid
At least one in sodium, sodium acetate, sodium formate, starch.
8. the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 1, it is characterised in that described acid
Alkali regulator is at least one in lactic acid powder or citric acid powder.
9. the preparation method for the ternary microorganism renovation agent of repairing concrete crack, it is characterised in that include following
Step:
By oxygen release agent, spore powder, nutrient substance and acid-base modifier it is in mass ratio (70%-95%): (1%-10%):
(5%-15%): (1-10%) pulverize, it is standby to grind；
Material mix homogeneously after grinding also carries out tabletting, and every time punching press adds the mixed-powder of 20-40mg, is fabricated to micro-
Grain, obtains described ternary microorganism renovation agent.
10. the preparation method of the ternary microorganism renovation agent for repairing concrete crack as claimed in claim 8, its feature
Being, the particle diameter of grinding is 100 mesh, a diameter of 3-10mm of described microgranule, a height of 2-10mm.
The application in repairing concrete crack of 11. 1 kinds of ternary microorganism renovation agents as described in claim 1 or 9.
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Cited By (6)
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| CN108102947A (en) * | 2017-12-13 | 2018-06-01 | 深圳大学 | One plant of aerobic-type efficient calcium mineralising bacillus and the application in concrete reparation |
| JP2018172234A (en) * | 2017-03-31 | 2018-11-08 | 国立大学法人愛媛大学 | Microbe-encapsulated microcapsule, cement admixture including microbe-encapsulated microcapsule, and repair material of cement-based structure |
| CN109574527A (en) * | 2018-12-15 | 2019-04-05 | 华南理工大学 | A kind of functionality cement selfreparing manufactured aggregate and preparation method thereof |
| CN110272249A (en) * | 2019-07-31 | 2019-09-24 | 海南华盛混凝土有限公司 | A kind of Machine-made Sand C35 pump concrete and preparation method thereof |
| CN111825422A (en) * | 2020-06-11 | 2020-10-27 | 天津新滨工程技术检测有限公司 | Concrete crack repairing technology based on microorganisms |
| CN112225480A (en) * | 2020-11-09 | 2021-01-15 | 深圳信息职业技术学院 | Reinforced regeneration surface porous material, preparation method and application thereof, and device for preparing reinforced regeneration surface porous material |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104403959A (en) * | 2014-09-18 | 2015-03-11 | 深圳大学 | Efficient calcium mineralized alkalophilic bacillus strain and application thereof |
| CN205088015U (en) * | 2015-11-05 | 2016-03-16 | 北京博汇特环保科技有限公司 | Bionical pasture and water to purification of black and odorous watercourse quality of water |
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| CN104403959A (en) * | 2014-09-18 | 2015-03-11 | 深圳大学 | Efficient calcium mineralized alkalophilic bacillus strain and application thereof |
| CN205088015U (en) * | 2015-11-05 | 2016-03-16 | 北京博汇特环保科技有限公司 | Bionical pasture and water to purification of black and odorous watercourse quality of water |
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| JP2018172234A (en) * | 2017-03-31 | 2018-11-08 | 国立大学法人愛媛大学 | Microbe-encapsulated microcapsule, cement admixture including microbe-encapsulated microcapsule, and repair material of cement-based structure |
| CN108102947A (en) * | 2017-12-13 | 2018-06-01 | 深圳大学 | One plant of aerobic-type efficient calcium mineralising bacillus and the application in concrete reparation |
| CN108102947B (en) * | 2017-12-13 | 2021-04-02 | 深圳大学 | Aerobic high-efficiency calcium-mineralized bacillus and application thereof in concrete repair |
| CN109574527A (en) * | 2018-12-15 | 2019-04-05 | 华南理工大学 | A kind of functionality cement selfreparing manufactured aggregate and preparation method thereof |
| CN109574527B (en) * | 2018-12-15 | 2021-09-21 | 华南理工大学 | Functional cement self-repairing artificial aggregate and preparation method thereof |
| CN110272249A (en) * | 2019-07-31 | 2019-09-24 | 海南华盛混凝土有限公司 | A kind of Machine-made Sand C35 pump concrete and preparation method thereof |
| CN111825422A (en) * | 2020-06-11 | 2020-10-27 | 天津新滨工程技术检测有限公司 | Concrete crack repairing technology based on microorganisms |
| CN112225480A (en) * | 2020-11-09 | 2021-01-15 | 深圳信息职业技术学院 | Reinforced regeneration surface porous material, preparation method and application thereof, and device for preparing reinforced regeneration surface porous material |
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