CN204550560U - Device is searched outside a kind of embryoid body - Google Patents
Device is searched outside a kind of embryoid body Download PDFInfo
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- CN204550560U CN204550560U CN201520159508.6U CN201520159508U CN204550560U CN 204550560 U CN204550560 U CN 204550560U CN 201520159508 U CN201520159508 U CN 201520159508U CN 204550560 U CN204550560 U CN 204550560U
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- embryo
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- filter
- embryoid body
- suction nozzle
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Abstract
The utility model relates to experiment auxiliary device field, is specifically related to search device outside a kind of embryoid body, comprises filter and glass needle, described filter comprises the first termination and two terminations, described glass needle is connected with described second termination, also comprises suction nozzle, and described suction nozzle is connected with described first termination.This device is small and exquisite flexibly, it is simple and easy to operate, easy to carry, cheap, be adapted at vast laboratory and promote the use of, greatly improve the external working efficiency searched of mammal embryo, solve body early embryo or ovocyte is searched or easily lose in moving process or the problem such as not easy to operate.
Description
Technical field
The utility model belongs to embryo biotechnology field, is specifically related to search device outside a kind of embryoid body.
Background technology
Embryo biotechnology is the important directions that Mammals breeds scientific research.In embryo biotechnology research, the operation of embryo operation, ovocyte is its important technological operation link, and how effectively control embryo or ovocyte are important steps in embryo biotechnology research.Except effectively controlling except embryo or ovocyte with plant and instrument such as micrurgy instrument, pipettors, people also realize the control of embryo with manual type inspection fetus device.But manual type embryo search the number of the wayward operation liquid measure of device or embryo, ovocyte search quantity, suction is uneven, easily causes personal errors, causes the loss of embryo or ovocyte.
Along with the development of science and technology, the new and high technology developments such as embryo transfer, embryo IVC, nuclear transplantation, and mammal ovocyte, embryo early time treatment more and more frequent.No matter in embryo biotechnology scientific research, or in mammal embryo production practice, an effective service platform must be possessed and process or control embryo or ovocyte quantity and quality.Therefore, design a kind of cost low, be convenient to control, easy handling, do not pollute, and the external device of searching of the mammal embryo that can enhance productivity seems particularly important.
Utility model content
For solving in prior art the above-mentioned technical problem existed, the utility model provides outside a kind of mouth suction embryoid body and searches device, is intended to solve body early embryo or ovocyte is searched or easily lose in moving process or the problem such as not easy to operate.The purpose of this utility model is achieved through the following technical solutions:
Search device outside a kind of embryoid body, comprise filter and glass needle, described filter comprises the first termination and two terminations, and described glass needle is connected with described second termination, also comprises suction nozzle, and described suction nozzle is connected with described first termination.
Concrete, between described first termination and described suction nozzle, be provided with the first flexible pipe.
Concrete, between described second termination and described glass needle, be provided with the second flexible pipe.
Concrete, described filter is syringe-driven filter.
Concrete, described flexible pipe is rubber hose.
Concrete, described glass needle port arranges inspection embryo mouth.
Further, described suction nozzle is made up of 1 200 μ l sterile pipette tip, directly inserts in the first flexible pipe, and it is that mouth inhales position that suction nozzle exposes 1.5 ~ 2.0cm, and suction nozzle insertion portion and the first hose connection place seal naturally.
Further, described first flexible pipe is little flexible pipe, is made up of, has elasticity elastomeric material, internal diameter 0.3mm, thickness 0.1mm, and length 10 ~ 15 cm, the other end is connected with filter.
Further, described filter is the syringe-driven filter that tetrafluoroethylene is formed, filter diameter 15 ~ 25mm, filter sizes 0.20 ~ 0.22 μm, filter membrane area 1.7 ~ 4.8cm
2.
Further, the described filter the other end is connected with the second flexible pipe, and described second flexible pipe is large flexible pipe, is made up of, has elasticity elastomeric material, internal diameter 0.5mm, thickness 0.1mm, length 10 ~ 20cm, and the other end is connected with inspection embryo glass needle.
Further, described inspection embryo glass needle is drawn by Glass tubing calcination on spirit lamp and forms, internal diameter 0.6 ~ 0.7mm, thickness 0.1mm.Described glass needle does not draw one end and is connected with large flexible pipe, naturally seals, length 8 ~ 10cm; The other end is tapered at pulling process, length 2 ~ 4cm, blocks at diameter 150 ~ 250 μm of place's emery wheels, and truncated position is inspection embryo mouth.
Search device outside a kind of embryoid body that the utility model relates to, in enforcement embryo or Oocyte quality evaluation with election process, be convenient to embryo or the ovocyte operation control of liquid measure and the determination of embryo's quantity.With mouth by suction nozzle controlled suction size, realize the release choosing or operate liquid controlling embryo.First before searching embryo, utilize the wicking action of inspection embryo glass needle, automatic feeding fluid in operation liquid, then utilizes mouth suction search or pipette embryo under the microscope.This external inspection fetus device is not only applicable to searching of cattle and sheep embryo or ovocyte or mobile, the liquid that changes when being also suitable for grade estimation and the vitro culture of cattle and sheep embryo or ovocyte operates, also be suitable for the embryo transfer operation of the meiofaunas such as mouse, rabbit, sheep, pig simultaneously, be easy in the research and development of mammal embryo biotechnology or use in producing.
The beneficial effects of the utility model are:
This device is small and exquisite flexibly, it is simple and easy to operate, easy to carry, cheap, be adapted at vast laboratory and promote the use of, greatly improve the external working efficiency searched of animal embryo, solve body early embryo or ovocyte is searched or easily lose in moving process or the problem such as not easy to operate.
Accompanying drawing explanation
Fig. 1 searches apparatus structure schematic diagram outside a kind of embryoid body of the utility model.
Embodiment
Below in conjunction with accompanying drawing, the technical solution of the utility model is described in further detail.
Search device outside a kind of mouth suction cattle and sheep embryoid body, described in search device for connection mode linear structure successively, there is suction nozzle 1, little flexible pipe 2, filter 3, large flexible pipe 4, inspection embryo glass needle 5; Described little flexible pipe 2 one end is connected with suction nozzle 1, and the other end is connected with filter 3 first port 6; Described large flexible pipe 4 one end is connected with filter 3 large port 7, and the other end is connected with inspection embryo glass needle 5; Described glass needle 5 is drawn by Glass tubing calcination on spirit lamp and forms.
Described suction nozzle 1 is made up of 1 200 μ l sterile pipette tip, directly inserts in little flexible pipe 2, and it is that mouth inhales position that suction nozzle 1 exposes 1.5cm, and suction nozzle 1 insertion portion and little flexible pipe 2 junction seal naturally.Described little flexible pipe 2 is made up of elastomeric material, has elasticity, internal diameter 0.3mm, thickness 0.1mm, length 13cm, and the other end is connected with filter 3.The syringe-driven filter that described filter 3 is formed for tetrafluoroethylene, filter 3 diameter 20mm, filter sizes 0.20 μm, filter membrane area 3.1cm
2.Described filter 3 the other end is connected with large flexible pipe 4, and described large flexible pipe 4 is made up of elastomeric material, has elasticity, internal diameter 0.5mm, thickness 0.1mm, length 20cm, and the other end is connected with inspection embryo glass needle 5.Described inspection embryo glass needle 5 is drawn by Glass tubing calcination on spirit lamp and forms, internal diameter 0.6mm, thickness 0.1mm.Described glass needle 5 does not draw one end and is connected with large flexible pipe 4, naturally seals, length 10cm; The other end is tapered at pulling process, length 3cm, blocks at diameter 200 μm of place's emery wheels, and truncated position is inspection embryo mouth 8.
Search device outside a kind of embryoid body that the utility model relates to, in enforcement embryo or Oocyte quality evaluation with election process, be convenient to embryo or the ovocyte operation control of liquid measure and the determination of embryo's quantity.With mouth by suction nozzle 1 controlled suction size, realize the release choosing or operate liquid controlling embryo.First, before searching embryo, utilize the wicking action of inspection embryo glass needle 5, automatic feeding fluid in operation liquid, then utilizes mouth suction search or pipette embryo under the microscope.This external inspection fetus device is not only applicable to searching of cattle and sheep embryo or ovocyte or mobile, the liquid that changes when being also suitable for grade estimation and the vitro culture of cattle and sheep embryo or ovocyte operates, also be suitable for the embryo transfer operation of the meiofaunas such as mouse, rabbit, sheep, pig simultaneously, be easy in the research and development of mammal embryo biotechnology or use in producing.
Finally it should be noted that, the foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, although be described in detail the utility model with reference to previous embodiment, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.All within spirit of the present utility model and principle, any amendment done, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (6)
1. search a device outside embryoid body, comprise filter and glass needle, described filter comprises the first termination and two terminations, and described glass needle is connected with described second termination, it is characterized in that: also comprise suction nozzle, and described suction nozzle is connected with described first termination.
2. search device outside a kind of embryoid body according to claim 1, it is characterized in that: between described first termination and described suction nozzle, be provided with the first flexible pipe.
3. search device outside a kind of embryoid body according to claim 1, it is characterized in that: between described second termination and described glass needle, be provided with the second flexible pipe.
4. search device outside a kind of embryoid body according to claim 1, it is characterized in that: described filter is syringe-driven filter.
5. search device outside a kind of embryoid body according to Claims 2 or 3, it is characterized in that: described flexible pipe is rubber hose.
6. search device outside a kind of embryoid body according to claim 1, it is characterized in that: described glass needle port arranges inspection embryo mouth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520159508.6U CN204550560U (en) | 2015-03-20 | 2015-03-20 | Device is searched outside a kind of embryoid body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520159508.6U CN204550560U (en) | 2015-03-20 | 2015-03-20 | Device is searched outside a kind of embryoid body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204550560U true CN204550560U (en) | 2015-08-12 |
Family
ID=53825696
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520159508.6U Expired - Fee Related CN204550560U (en) | 2015-03-20 | 2015-03-20 | Device is searched outside a kind of embryoid body |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204550560U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257229A (en) * | 2019-07-08 | 2019-09-20 | 河南省农业科学院畜牧兽医研究所 | It is a kind of simply to inhale fetus device and preparation method thereof |
-
2015
- 2015-03-20 CN CN201520159508.6U patent/CN204550560U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257229A (en) * | 2019-07-08 | 2019-09-20 | 河南省农业科学院畜牧兽医研究所 | It is a kind of simply to inhale fetus device and preparation method thereof |
| CN110257229B (en) * | 2019-07-08 | 2023-11-07 | 河南省农业科学院畜牧兽医研究所 | Simple embryo suction device and manufacturing method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150812 Termination date: 20190320 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |